Infant and child formula useful for enhancing and / or improving individual social behavior

Abstract

The present invention provides infant and child formulas useful for enhancing and / or improving the social behavior of an individual. The invention firstly provides the infant formula food, the infant formula food is infant formula food, infant formula food or child formula food, the infant formula food comprises DHA, ARA and phospholipid, the phospholipid is sphingomyelin and / or lecithin, the mass ratio of DHA to phospholipid is (0.5-13): 1, and the mass ratio of ARA to phospholipid is (0.5-17): 1. The formulae of the present invention contribute to enhancing and / or improving the social behavior of an individual.

Description

Technical Field

[0001] The present invention belongs to the technical field of infant and children's formula food, and specifically relates to an infant and children's formula food containing DHA, ARA and phospholipids, which helps to enhance and / or improve individual social behavior and its application. Background Art

[0002] In the field of infant and children's formula, a core technical issue is how to design and prepare formula foods that can simulate the nutritional components and functions of breast milk to promote the healthy growth of infants and young children. Breast milk not only provides essential nutrients, but also contains a variety of bioactive ingredients that have an important impact on the neurodevelopment and behavioral performance of infants and young children. However, when breast milk is lacking or insufficient, existing infant and children's formula foods often find it difficult to fully replicate these complex components and functions in breast milk, especially in promoting the development of social behavior.

[0003] Social behavior refers to an individual's ability to interact with people around him or her and to take care of himself or herself. It is one of the attributes for assessing the developmental behavior level of children aged 0 to 6 years old (under 7 years old).

[0004] DHA, the full name of which is docosahexaenoic acid, is an important Omega-3 polyunsaturated fatty acid that plays a significant role in improving intelligence. It plays a key role in brain development and function, especially in infancy. DHA is a key component of the gray matter of the brain and is essential for the growth and connection of nerve cells, thereby promoting brain development. In addition, DHA is also involved in the transmission of neurotransmitters, neural development, myelination, and membrane receptor function, and has an important impact on the development of cognitive function. Studies have also shown that DHA supplementation can improve learning ability and memory, especially in infancy, where the effect of DHA supplementation on brain development is more significant. Therefore, DHA is known as "brain gold" and plays an important role in promoting intellectual development and maintaining cognitive function. DHA exists naturally in breast milk, especially in colostrum, where the content of DHA is the richest. DHA in breast milk mainly exists in the form of SN-2, which is easier to be absorbed and utilized by infants.

[0005] ARA, or arachidonic acid, is a long-chain polyunsaturated fatty acid of the Omega-6 family. It is found in breast milk and is essential for the development of the infant's brain and nervous system. In infant formula, ARA works together with DHA to help enhance and improve an individual's social behavior. Studies have shown that infants who consume milk powder containing ARA and DHA score higher in problem-solving tests, have improved visual acuity, and have a fatty acid composition in their blood that is closer to that of breastfed babies. ARA has a positive effect on the neurodevelopment, cognitive function, and behavioral development of infants.

[0006] Sphingomyelin is an important sphingolipid substance and a component of nerve cell membranes. It plays an important role in transmitting information to nerve cells and is also an important nutrient for promoting brain nerve development. It is also an important component of the myelin sheath, which has the function of protecting nerve cells and promoting their development. It has a positive effect on the development of brain behavior and attention awareness. Supplementing sphingomyelin is very helpful for brain development. In addition, sphingomyelin also supports the formation of brain myelin, which is a process closely related to cognitive maturation. Studies have shown that sphingomyelin may affect the cognitive development of healthy children by regulating the formation of oligodendrocytes and axon myelin.

[0007] Lecithin is a yellow-brown oily substance found in animal and plant tissues and egg yolks. Its main components include phosphoric acid, choline, fatty acids, glycerol, etc. It is known as the "third nutrient" alongside protein and vitamins, and has a significant effect on intelligence. It can provide the choline needed by the brain and help synthesize acetylcholine, an important neurotransmitter that is essential for improving the activation of brain cells, memory and intelligence. Lecithin also helps to enhance memory, concentration, and improve the brain's thinking ability. In addition, lecithin is also present in breast milk. It is one of the main components of the milk fat globule membrane structure and plays an important role in the growth and development of infants, especially the development of brain nerves.

[0008] After searching, there are few reports in the prior art on whether diet affects individual social behavior and what kind of impact it has on individual social behavior. Summary of the invention

[0009] An object of the present invention is to provide an infant and children's formula that helps to greatly enhance and / or improve the social behavior of an individual.

[0010] Another object of the present invention is to provide related applications of the infant and children's formula food.

[0011] The present invention has found that polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and arachidonic acid (ARA), and a composition further comprising sphingomyelin (SM) and / or lecithin (i.e., a composition comprising DHA, ARA, sphingomyelin and / or lecithin), can affect the social behavior of an individual. In particular, when DHA, ARA and sphingomyelin, especially milk phospholipids (milk SM), are combined in a certain proportion, they have a synergistic effect on enhancing and / or improving the social behavior of an individual.

[0012] On the one hand, the present invention provides a pediatric formula food, which is an infant formula food, a toddler formula food or a children's formula food, wherein the total protein content of the pediatric formula food is 10-30g / 100g, the fat content is 10-35g / 100g, the dietary fiber content is 0-6.5g / 100g, and the carbohydrate content is 45-60g / 100g; and the pediatric formula food contains DHA, ARA and phospholipids, wherein the phospholipids are sphingomyelin and / or lecithin, wherein the mass ratio of DHA to phospholipids is 0.5-13:1, and the mass ratio of ARA to phospholipids is 0.5-17:1.

[0013] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to ARA is 0.2-5:1.

[0014] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to ARA is 0.3-3:1.

[0015] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to ARA is preferably 0.5-2.5:1.

[0016] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to ARA is preferably 0.5-2:1.

[0017] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to ARA is preferably 0.5-1.5:1, for example 0.5:1, 0.6:1, 0.67:1, 0.8:1, 1:1, 1.1:1, 1.2:1, 1.3:1 or 1.4:1.

[0018] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the mass ratio of DHA to phospholipids is 0.5-8:1, for example 0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1 or 8:1.

[0019] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the mass ratio of ARA to phospholipids is 0.5-10:1, for example 0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1 or 10:1.

[0020] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, when the phospholipids are sphingomyelin and lecithin, the amount of the phospholipids is the total amount of sphingomyelin and lecithin, wherein sphingomyelin and lecithin can be combined in any proportion.

[0021] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the phospholipid is sphingomyelin.

[0022] According to some specific embodiments of the present invention, in the pediatric formula food of the present invention, the sphingomyelin is milk phospholipids.

[0023] According to some specific embodiments of the present invention, the pediatric formula includes DHA, ARA and milk lecithin.

[0024] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the raw material for providing the DHA can be a commercially available DHA raw material with higher purity, or it can be a DHA oil and / or powder derived from one or a combination of two or more of algae, tuna oil, fish oil, milk-derived DHA from feed nutritional regulation and biotransformation, egg yolk extract and krill oil; wherein the algae can be one or a combination of two or more of Schizochytrium, Okenichytrium and Crypthecodinium. Unless otherwise specified, the ratio of the DHA to other functional components in the pediatric formula food of the present invention is based on the content of DHA in the raw material.

[0025] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the raw material for providing the ARA can be a commercially available ARA raw material with higher purity, or can be one or more raw materials derived from algae and other oils containing ARA. Unless otherwise specified, the ratio of the ARA to other functional components in the pediatric formula food of the present invention is based on the content of ARA in the raw material.

[0026] According to a specific embodiment of the present invention, in the children's formula food of the present invention, the raw material for providing the sphingomyelin can be a commercially available sphingomyelin raw material with higher purity. The sphingomyelin and / or lecithin can come from cow's milk, goat's milk and their products, including but not limited to whole milk powder, skim milk powder, casein and whey protein powder, preferably whey protein powder. Unless otherwise specified, the ratio of the sphingomyelin to other functional components in the children's formula food of the present invention is based on the content of sphingomyelin in the raw material. When the sphingomyelin described in the children's formula food of the present invention is milk phospholipids, its ratio to other functional components is based on the content of milk phospholipids in the raw material.

[0027] According to a specific embodiment of the present invention, the amount of sphingomyelin contained in the pediatric formula food of the present invention is 20-138 mg / 100 g of food dry matter.

[0028] According to a specific embodiment of the present invention, the content of DHA and ARA in the pediatric formula of the present invention can be determined according to the ratio with sphingomyelin.

[0029] According to a specific embodiment of the present invention, the pediatric formula food of the present invention preferably contains a total amount of DHA and ARA of 100-180 mg / 100 g of food dry matter.

[0030] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the dietary fiber includes one or more of galacto-oligosaccharides and fructo-oligosaccharides.

[0031] In addition, the pediatric formula of the present invention may be in the form of a solid composition or a liquid composition.

[0032] In a specific embodiment, the pediatric formula food of the present invention is a dairy product, which can be a solid dairy product or a liquid dairy product. Specifically, it can be in the form of cheese, liquid dairy product, milk flakes or milk powder.

[0033] In a more specific embodiment, the pediatric formula food of the present invention is milk powder, and is prepared by adding DHA, ARA and sphingomyelin and / or lecithin to a basic formula powder. And, more specifically, the basic formula powder is an infant formula powder.

[0034] It is understandable that for different types of pediatric formula foods, the content of each functional substance in the final product should be appropriately adjusted within the range permitted by relevant standards and specifications.

[0035] In another aspect of the present invention, a method for preparing the pediatric formula food of the present invention is provided, which comprises: adding DHA, ARA and sphingomyelin and / or lecithin to food raw materials, and adjusting the ratio of DHA, ARA and sphingomyelin and / or lecithin so that, in terms of weight ratio, the mass ratio of DHA to phospholipid is 0.5-13:1, the mass ratio of ARA to phospholipid is 0.5-17:1, and the mass ratio of DHA to ARA is 0.2-5:1.

[0036] In the present invention, the sphingomyelin and / or lecithin are derived from cow's milk, goat's milk and their products, including but not limited to whole milk powder, skim milk powder, casein and whey protein powder, preferably whey protein powder.

[0037] According to a specific embodiment of the present invention, the pediatric formula food of the present invention, in addition to the above-mentioned components, may also include conventional components of formula food, for example, may also include a compound nutrient package.

[0038] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the raw materials providing total protein include: raw milk (cow / sheep), whole milk powder (cow / sheep), skimmed milk powder (cow / sheep), whey protein powder (cow / sheep), desalted whey powder (cow / sheep), β-casein, etc. One or more.

[0039] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the raw materials providing fat include vegetable oil or OPO structured fat in addition to the basic raw materials containing milk fat.

[0040] According to a specific embodiment of the present invention, in the pediatric formula food of the present invention, the raw materials providing carbohydrates include: 0 to 350 parts of lactose, and / or the above-mentioned protein raw materials containing lactose.

[0041] According to some specific embodiments of the present invention, in the children's formula food of the present invention, a part of the carbohydrates come from basic raw materials containing lactose such as milk, whole milk powder and / or skim milk powder, etc., and lactose raw materials should be added to provide carbohydrates. That is, in the children's formula food of the present invention, the raw materials for providing carbohydrates include raw material lactose in addition to the basic raw materials containing lactose. Preferably, based on 1000 parts by weight of the children's formula food, its raw materials include: 125 to 325 parts by weight of lactose. The specific amount of lactose added can be adjusted within the range so that the carbohydrate content of the children's formula food of the present invention is 45 to 60g / 100g.

[0042] According to a specific embodiment of the present invention, the raw materials of the pediatric formula food of the present invention may also include one or a combination of two or more of appropriate nucleotides, lactoferrin, lutein, etc. Preferably, based on 1000 parts by weight of the pediatric formula food of the present invention, the raw materials include: 0 to 1 part by weight of nucleotides and 0 to 8.5 parts by weight of lactoferrin.

[0043] According to a specific embodiment of the present invention, the raw materials of the pediatric formula food of the present invention may also include compound nutrients including calcium powder, vitamins and minerals. The compound nutrients are a combination of nutrients that meet national standards, and different addition amounts are used according to different formulas. Preferably, based on 1000 parts by weight of the formula food, the raw materials include: 7 to 25 parts by weight of compound nutrients including calcium powder, vitamins and minerals. More preferably, the compound nutrients are added in the form of at least a compound vitamin package, calcium powder, and a mineral nutrient package. Specifically, the compound nutrients are preferably added in the form of the following nutrient packages:

[0044] 1) Compound vitamin nutrient pack, the content of each component in each gram of compound vitamin nutrient pack is as follows:

[0045] Taurine: 0-340 mg

[0046] Vitamin A: 1200~7000μgRE

[0047] Vitamin D: 20-112 μg

[0048] Vitamin B1: 1500-14000 μg

[0049] Vitamin B2: 3500-14000 μg

[0050] Vitamin B6: 1000-7000 μg

[0051] Vitamin B 12 :8~30μg

[0052] Vitamin K1: 200-750 μg

[0053] Vitamin C: 0-800 mg

[0054] Vitamin E: 10-70 mg α-TE

[0055] Nicotinamide: 10,000-47,000 μg

[0056] Folic acid: 420-3000 μg

[0057] Biotin: 38-245 μg

[0058] Pantothenic acid: 6000-60000 μg

[0059] Inositol: 0-250 mg

[0060] L-Carnitine: 0-150 mg

[0061] 2) Mineral 2 Nutrition Pack, the content of each component in each gram of Mineral 2 Nutrition Pack is as follows:

[0062] Calcium: 120-6000 mg

[0063] Phosphorus: 0-150 mg

[0064] 3) Mineral nutrition pack, the content of each component in each gram of mineral nutrition pack is as follows:

[0065] Iron: 25-135 mg

[0066] Zinc: 23-175 mg

[0067] Copper: 2600-12000 μg

[0068] Iodine: 0-995 μg

[0069] Selenium: 0-200 μg

[0070] Manganese: 0~15000μg

[0071] 4) Compound magnesium chloride nutrient pack, each gram of compound magnesium chloride nutrient pack contains:

[0072] Magnesium: 80-2800 mg

[0073] 5) Compound potassium chloride nutrient pack, each gram of compound potassium chloride nutrient pack contains:

[0074] Potassium: 0-580 mg;

[0075] 6) Choline chloride nutrient pack, each gram of choline chloride nutrient pack contains:

[0076] Choline: 300-1500 mg;

[0077] Preferably, based on 1000 parts by weight of the pediatric formula food, the added amount of the compound vitamin nutrient package is 2 to 4 parts by weight, the added amount of the mineral 2 nutrient package is 2 to 18 parts by weight, the added amount of the mineral 1 nutrient package is 0.5 to 3 parts by weight, the added amount of the compound magnesium chloride nutrient package is 0 to 2 parts by weight, the added amount of the compound potassium chloride nutrient package is 0 to 4.5 parts by weight, and the added amount of choline chloride nutrient is 0 to 2 parts by weight, and the base material of each nutrient package is preferably lactose or sodium L-ascorbate.

[0078] In the pediatric formula food of the present invention, the raw materials lecithin and / or anhydrous butter are mainly used for forming powder particles in the spray drying process, and the lecithin can be soybean lecithin and / or egg lecithin. The amount of lecithin and anhydrous butter is small, but they also contribute to the fat content in the formula food product.

[0079] According to a preferred embodiment of the present invention, the infant formula food of the present invention comprises:

[0080]

[0081]

[0082] It is understood that the specific amount of each raw material in the pediatric formula food of the present invention should be adjusted and determined under the premise of meeting the requirements of the formula food product index. In the pediatric formula food of the present invention, the product performance indicators not described or listed in detail should be implemented in accordance with the national standards for infant or children's formula food and relevant standards and regulations.

[0083] In the pediatric formula food of the present invention, each raw material can be commercially available, and the selection of each raw material should comply with the requirements of relevant standards, wherein the composition should also meet the requirements of the present invention. In addition, the compounded nutrients can also be compounded by themselves. The present invention only uses "compounding" for the convenience of expression, and does not mean that the components in the compound must be mixed together before use. Each raw material should be added and used under the premise of meeting relevant laws and regulations.

[0084] On the other hand, the present invention also provides a method for preparing the pediatric formula food, such as pediatric formula milk powder. The preparation process mainly includes: batching, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product. Specifically, the method for preparing the pediatric formula milk powder containing DHA, ARA, sphingomyelin and / or lecithin composition of the present invention includes:

[0085] 1) Milk coarse filtration: After the milk is coarsely filtered and degassed in the balance cylinder, it is preheated by a plate heat exchanger and then separated from impurities by a separator.

[0086] 2) Milk homogenization and sterilization: After removing impurities, part of the raw milk enters the homogenizer for homogenization, and the other part is not homogenized. After homogenization, the two are mixed and enter the sterilization system for sterilization.

[0087] 3) Powder addition: Various powder raw materials are measured according to the formula and added into the powder mixing tank through the air delivery system for storage.

[0088] 4) Vacuum powder suction: Various powder raw materials in the powder mixing tank are sucked into the vacuum mixing tank through the vacuum system.

[0089] 5) Melting and oil preparation: Put the grease specified in the formula into the carburettor room according to the formula requirements. The temperature in the carburettor room should be maintained at 50-90°C. After the oil is dissolved, it is pumped into the mixed oil storage tank through the oil pump and flow meter according to the formula ratio requirements.

[0090] 6) Storage of mixed oil: The mixed oil is stored in an oil storage tank at a temperature of 40-50°C and the storage time is less than 12 hours to prevent fat oxidation.

[0091] 7) Weighing: Pump the mixed oil into the mixing tank through the oil pump according to the formula requirements.

[0092] 8) Dissolved and added nutrients: Calcium powder, minerals, vitamins, etc. are added separately with 100-200 kg of pure water. After dissolving them separately, they are pumped into the wet mixing tank. After each one is added, rinse the addition tank and pipeline with 100 kg of pure water.

[0093] 9) Filtration: The mixed liquid is filtered through a filter to remove physical impurities that may be introduced into the raw materials.

[0094] 10) Homogenization: The mixed liquid is homogenized by a homogenizer. The first-stage homogenization pressure is 105±5 bar and the second-stage homogenization pressure is 32±3 bar. The fat globules are mechanically treated and dispersed into uniform fat globules.

[0095] 11) Cooling and storage: The homogenized liquid enters the plate heat exchanger for cooling: it is cooled to below 20°C and temporarily stored in the pre-storage tank. It enters the next process within 6 hours, and the agitator is turned on according to the set requirements.

[0096] 12) Concentration and sterilization: Double-effect concentration is used during production, the sterilization temperature is ≥83°C, and the sterilization time is 25 seconds. The discharge concentration is 48% to 52% dry matter.

[0097] 13) Concentrated milk storage, preheating filtration, spray drying: The concentrated milk is temporarily stored in the concentrated milk balance tank. It is preheated to 60-70℃ by the scraper preheater. After preheating, the material is filtered through a filter with a pore size of 1mm, and then pumped into the drying tower for spray drying by a high-pressure pump. The fine powder is agglomerated at the top of the tower or in the fluidized bed as required. Inlet air temperature: 165-180℃, exhaust air temperature 75-90℃, high-pressure pump pressure 160-210bar, tower negative pressure -4--2mbar.

[0098] 14) Fluidized bed drying and cooling: The powder coming out of the drying tower is dried again in a fluidized bed (primary) and then cooled to 25-30°C in a fluidized bed (secondary). At the same time, the lecithin is mixed with the carrier and heated to 60-65°C. Under the action of compressed air, it is evenly dispersed on the surface of the powder, so that the powder particles agglomerate to increase its particle size and solubility.

[0099] 15) Packaging: The flour-making workshop staff weighs DHA, ARA lactoferrin and bifidobacteria and packs them in bags according to the formula requirements.

[0100] 16) Dry mixing: Mix the weighed DHA, ARA, lactoferrin and milk powder in a dry mixer.

[0101] 17) Powder screening: Use a vibrating screen to make the particle size of the milk powder uniform and discard the powder residue.

[0102] 18) Powder output: Collect the powder in a sterilized powder box and transport it from the powder output room to the powder loading room.

[0103] 19) Powdering: Pour the milk powder into the powder storage tank on the large and small packaging machines according to the packaging requirements.

[0104] 20) Packaging: 800g automatic packaging machine nitrogen filling packaging. The oxygen content during nitrogen filling is less than 1%. 900g iron cans automatically filled with nitrogen packaging oxygen content less than 5%.

[0105] 21) Packing: Put the packaged sachets into cartons and add the powder spoon, then seal with a carton sealing machine.

[0106] 22) Finished product inspection: Sampling inspection is carried out on the packaged products according to the inspection plan.

[0107] 23) Storage: Products that have passed the inspection shall be stored in warehouses at room temperature with humidity ≤ 65%.

[0108] On the other hand, the present invention also provides the use of the pediatric formula food in preparing a product that helps to enhance and / or improve the social behavior of an individual. The product can be the pediatric formula food of any of the aforementioned schemes of the present invention, or can be further prepared from other products based on the pediatric formula food of the present invention.

[0109] According to some specific embodiments of the present invention, the functional components in the pediatric formula food are composed of DHA, ARA and milk lecithin, and the proportions of the components can be any of the above proportions of the present invention. The efficacy refers to enhancing and / or improving the social behavior of an individual.

[0110] According to a specific embodiment of the present invention, in the present invention, the enhancing and / or improving the social behavior of an individual includes: enhancing the social willingness and / or social ability of an individual.

[0111] According to a specific embodiment of the present invention, in the present invention, the social ability includes social adaptability (the ability of an individual to respond to and adapt to the social environment and / or social needs around him / her).

[0112] According to a specific embodiment of the present invention, the pediatric formula food of the present invention is used in the preparation of a product for improving and / or improving the social behavior of an individual, wherein the individual is an infant, a toddler or a child. The infant and children's formula food of the present invention can improve the low social behavior of an individual.

[0113] In the present invention, unless otherwise specified, the infant refers to an infant of 0-12 months, the toddler refers to an infant of 1-3 years old, and the child refers to a minor, particularly a child of 0 to 6 years old (under 7 years old).

[0114] The pediatric formula food provided by the present invention comprises polyunsaturated fatty acids DHA, ARA and phospholipids, which are helpful to enhance and / or improve the social behavior of an individual, and these functional components have a certain synergistic effect in enhancing and / or improving the social behavior of an individual. BRIEF DESCRIPTION OF THE DRAWINGS

[0115] Figure 1 The results of learning rate detection of each experimental group of PUFAs are shown.

[0116] Figure 2 Shows the test results of memory rate of each experimental group of PUFAs.

[0117] Figure 3 The results of the detection of the convergent frequencies of each experimental group of PUFAs are shown.

[0118] Figure 4 The results of the detection of the approaching cumulative time of each experimental group of PUFAs are shown.

[0119] Figure 5 Results showing the body contact frequency of each experimental group of PUFAs.

[0120] Figure 6 The test results showing the cumulative time of physical contact for each experimental group of PUFAs.

[0121] Figure 7 The results of the relative movement frequency test of each experimental group of PUFAs are shown.

[0122] Figure 8 The results of the relative migration accumulation time of each experimental group of PUFAs are shown.

[0123] Fig. 9 The test results showed that PUFAs and SM synergistically affected the approach frequency of individuals.

[0124] Fig.10 The test results showed that PUFAs and SM synergistically affected the individual's approach accumulation time.

[0125] Fig.11 The results showed that PUFAs and SM synergistically affected the frequency of physical contact of individuals.

[0126] Fig.12 The results showed that PUFAs and SM synergistically affected the cumulative duration of physical exposure of individuals.

[0127] Fig.13 Results showing that PUFAs and SM synergistically affect the relative movement frequency of individuals.

[0128] Fig.14 The test results showed that PUFAs and SM synergistically affected the cumulative relative movement time of individuals. DETAILED DESCRIPTION

[0129] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing the specific embodiments rather than for limiting the scope of protection of the present invention.

[0130] When numerical ranges are given in the embodiments, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected unless otherwise specified in the present invention.

[0131] Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.

[0132] In addition to the specific methods, equipment, and materials used in the embodiments, based on the understanding of the prior art by those skilled in the art and the description of the present invention, any methods, equipment, and materials of the prior art that are similar or equivalent to the methods, equipment, and materials described in the embodiments of the present invention may also be used to implement the present invention.

[0133] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in the present invention all adopt conventional techniques in the technical field.

[0134] In each embodiment and experiment, the DHA, ARA and phospholipids used are all commercially available food grade raw materials. Unless otherwise specified, the DHA, ARA and phospholipids mentioned in the present invention are all calculated based on the effective content of the functional components DHA, ARA and phospholipids in the raw materials.

[0135] Example 1

[0136] The present embodiment provides a powdered formula food for infants and children, wherein the total protein content of the powdered formula food is 10.5 g / 100 g powder, the fat content is 27.9 g / 100 g powder, the carbohydrate content is 55.4 g / 100 g powder, the DHA content is 98 mg / 100 g, the sphingomyelin content is 38 mg / 100 g, and the mass ratio of DHA to phospholipids (i.e., sphingomyelin and / or lecithin) is 2.6:1.

[0137] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0138] 1000 parts of raw cow's milk, 310 parts of desalted whey powder (D90), 225 parts of edible vegetable blending oil, 245 parts of lactose, 24 parts of galacto-oligosaccharide, 27 parts of α-lactalbumin powder, 8 parts of oligofructose, 13 parts of casein, 5 parts of whey protein powder (rich in milk fat globule membrane), 3 parts of phospholipids, 28 parts of compound nutrients, wherein the compound nutrients include about 4 parts of compound vitamin nutrient package, about 2 parts of choline chloride nutrient package, about 19 parts of mineral 2 nutrient package, about 1 part of mineral 1 nutrient package, about 2 parts of magnesium chloride nutrient package, the base material of each nutrient package is lactose, 11 parts of DHA, 12 parts of ARA, and about 0.7 parts of nucleotides.

[0139] The specific preparation process of the infant formula milk powder of this embodiment is as follows:

[0140] 1) Milk coarse filtration: After the milk is coarsely filtered and degassed in the balance cylinder, it is preheated by a plate heat exchanger and then separated from impurities by a separator.

[0141] 2) Milk homogenization and sterilization: After removing impurities, part of the raw milk enters the homogenizer for homogenization, and the other part is not homogenized. After homogenization, the two parts are mixed and enter the sterilization system for sterilization.

[0142] 3) Powder addition: various powder raw materials are added to the powder mixing tank through the air delivery system after being measured according to the formula, and then sucked into the vacuum mixing tank through the vacuum system;

[0143] 4) Melting and preparing oil: put the oil specified in the formula into the oiling room according to the formula requirements. The temperature in the oiling room is maintained at 50-90°C. After the oil is dissolved, pump it into the mixed oil storage tank, and then pump the mixed oil into the mixing tank through the oil pump according to the formula requirements;

[0144] 5) Dissolving and adding nutrients: Dissolve calcium powder, vitamins, minerals and other nutrient packages separately with purified water, and add them to the mixing tank in sequence to obtain a mixed liquid.

[0145] 6) Filtration: The mixed liquid is filtered through a filter to remove physical impurities that may be introduced into the raw materials.

[0146] 7) Homogenization: The mixed liquid is homogenized by a homogenizer to mechanically treat the fat globules and disperse them into uniform fat globules.

[0147] 8) Cooling and storage: The homogenized liquid enters the plate heat exchanger for cooling: it is cooled to below 20°C and temporarily stored in the pre-storage tank. It enters the next process within 6 hours, and the agitator is turned on according to the set requirements.

[0148] 9) Concentration and sterilization: Double-effect concentration is used during production, the sterilization temperature is ≥83°C, and the sterilization time is 25 seconds. The discharge concentration is 50% dry matter.

[0149] 10) Concentrated milk storage, preheating filtration, spray drying: The concentrated milk is temporarily stored in the concentrated milk balance tank. It is preheated to 60°C by a scraper preheater. After preheating, the material is filtered through a filter with a pore size of 1mm, and then pumped into the drying tower for spray drying by a high-pressure pump. The fine powder is agglomerated at the top of the tower or in the fluidized bed as required. Inlet air temperature: 180°C, exhaust air temperature 86°C, high-pressure pump pressure 200bar, tower negative pressure about -4mba.

[0150] 11) Fluidized bed drying and cooling: The powder coming out of the drying tower is dried again in a fluidized bed (primary stage) and then cooled to 30°C in a fluidized bed (secondary stage) to obtain the main ingredient of milk powder.

[0151] 12) Packaging: According to the formula requirements, weigh the DHA, ARA or bifidobacteria and seal the bags for packaging.

[0152] 13) Dry mixing: Mix the weighed DHA, ARA or bifidobacteria with the main milk powder in a dry mixer.

[0153] 14) Powder screening: Use a vibrating screen to make the particle size of the milk powder uniform and discard the powder residue.

[0154] 15) Powder output: Collect the powder in a sterilized powder box and transport it from the powder output room to the powder loading room.

[0155] 16) Powdering: Pour the milk powder into the powder storage tank on the large and small packaging machines according to the packaging requirements.

[0156] 17) Packaging: 400g automatic packaging machine nitrogen filling packaging. The oxygen content during nitrogen filling is less than 1%. 900g iron cans automatically filled with nitrogen packaging oxygen content is less than 5%.

[0157] 18) Packing: Put the packaged sachets into cartons and add the powder spoon, then seal with a carton sealing machine.

[0158] 19) Finished product inspection: Sampling inspection is carried out on the packaged products according to the inspection plan.

[0159] 20) Storage: Products that have passed the inspection shall be stored in warehouses at room temperature with humidity ≤ 65%.

[0160] Example 2

[0161] The present embodiment provides a powdered formula food for infants and children, wherein the total protein content of the powdered formula food is 12.0 g / 100 g powder, the fat content is 23.0 g / 100 g powder, the carbohydrate content is 57.3 g / 100 g powder, the ARA content is 110 mg / 100 g powder, and the sphingomyelin content is 32 mg / 100 g, and the mass ratio of the ARA to the phospholipids (i.e., sphingomyelin and / or lecithin) is 3.5:1.

[0162] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0163] 2000 parts of raw cow's milk, 240 parts of desalted whey powder (D90), 190 parts of edible vegetable blending oil, 285 parts of lactose, 25 parts of galactooligosaccharide, 10 parts of whey protein powder, 5 parts of oligofructose, 2 parts of casein, 5 parts of whey protein powder (rich in milk fat globule membrane), 4 parts of phospholipids, 24 parts of compound nutrients, wherein the compound nutrients include about 3 parts of compound vitamin nutrition package, about 2 parts of choline chloride nutrition package, about 17 parts of mineral two nutrition package, about 1 part of mineral one nutrition package, about 1 part of magnesium chloride nutrition package, and the base materials of each nutrition package are lactose, 10 parts of DHA, 13 parts of ARA, and 0.6 parts of lactoferrin.

[0164] The specific preparation process of the infant and children's formula food of this embodiment is consistent with that of embodiment 1.

[0165] Example 3

[0166] The present embodiment provides a powdered formula food for infants and children, wherein the total protein content of the powdered formula food is 13.0 g / 100 g powder, the fat content is 23.3 g / 100 g powder, the carbohydrate content is 53.7 g / 100 g powder, the DHA content is 96 mg / 100 g powder, the ARA content is 120 mg / 100 g powder, and the ratio of DHA:ARA is 0.8:1.

[0167] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0168] 2200 parts of raw goat milk, 500 parts of desalted goat whey powder (D90), 150 parts of edible vegetable blending oil, 50 parts of galacto-oligosaccharide, 20 parts of fructo-oligosaccharide, 3 parts of phospholipids, 22 parts of compound nutrients, wherein the compound nutrients include about 4 parts of compound vitamin nutrient package, about 2 parts of choline chloride nutrient package, about 13 parts of mineral 2 nutrient package, about 1 part of mineral 1 nutrient package, about 1 part of magnesium chloride nutrient package and about 1 part of potassium chloride nutrient package, the base material of each nutrient package is lactose, 17 parts of DHA, 15 parts of ARA, 0.2 parts of bifidobacterium and 1 part of nucleotide.

[0169] The specific preparation process of the infant and children's formula food of this embodiment is consistent with that of embodiment 1.

[0170] Example 4

[0171] The present embodiment provides a powdered formula food for infants and children, wherein the total protein content of the powdered formula food is 13.0 g / 100 g powder, the fat content is 24.0 g / 100 g powder, the carbohydrate content is 56.4 g / 100 g powder, the DHA content is 150 mg / 100 g powder, the ARA content is 180 mg / 100 g powder, the phospholipid (i.e., sphingomyelin and / or lecithin) content is 10 mg / 100 g, the mass ratio of DHA to phospholipid (i.e., sphingomyelin and / or lecithin) is 15:1, and the mass ratio of ARA to phospholipid (i.e., sphingomyelin and / or lecithin) is 18:1.

[0172] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0173] 3200 parts of raw milk, 225 parts of skimmed milk powder, 140 parts of desalted whey powder (D90), 180 parts of edible vegetable blending oil, 295 parts of lactose, 14 parts of galacto-oligosaccharide, 6 parts of fructo-oligosaccharide, 4 parts of phospholipids, 21 parts of compound nutrients, wherein the compound nutrients include about 3 parts of compound vitamin nutrient package, about 2 parts of choline chloride nutrient package, about 10 parts of mineral 2 nutrient package, about 1 part of mineral 1 nutrient package, about 2 parts of magnesium chloride nutrient package and about 3 parts of potassium chloride nutrient package, the base material of each nutrient package is lactose, 17 parts of DHA, 20 parts of ARA and 0.8 parts of nucleotide.

[0174] The specific preparation process of the infant and children's formula food of this embodiment is consistent with that of embodiment 1.

[0175] Experimental study on the efficacy of nutritional composition in improving individual social ability

[0176] 1. Bee species and grouping

[0177] One-day-old Italian honey bees (Apis mellifera) were used, and the intervention methods of different experimental groups were shown in Table 1 based on the diet of feed honey bees.

[0178] Table 1

[0179]

[0180] 2. Basic honey bee training

[0181] 2.1 Diet preparation

[0182] Preparation of conventional diet: weigh 500 g sucrose and 10 g casein hydrolysate, dilute to 1 L with ultrapure water, stir to dissolve, filter and sterilize with a 0.22 μm filter membrane to obtain the 50% (w / v) sucrose solution containing 1% (w / v) casein hydrolysate required for the experiment.

[0183] Diet of treatment group: treatment reagent was added to the regular diet; the single-fed group was fed with a daily diet of 40 μL sugar water per bee, and the reagent was concentrated to 5 μL sterile sugar water at one time.

[0184] 2.2 Bee picking

[0185] Select honeycombs of appropriate age from the apiary, quickly transport them back to the laboratory, and place them in an incubator at 35°C and 60-80% humidity. Observe the status of the fledged bees every day from the second day (if they have not fledged, continue to culture), gently remove the fledged bees with tweezers and pack them into disposable transparent culture cups, 30 per cup, and pack enough cups according to the experimental needs.

[0186] 2.3 Bee pretreatment

[0187] The fledged bees were taken directly from the apiary, and the intestines were extracted. After adding 100ul PBS to each intestine and grinding, 50% glycerol was added in a 1:1 ratio and stored at -80°C.

[0188] Take a sterile 2mL centrifuge tube, add 990μL sterile PBS, add 10μL worker bee intestinal fluid, mix well, and finally use 50% (w / v) sterile sucrose solution to make up to 2mL mark, shake and mix well. Feed each cup of newly emerged bees with 1 tube of the above mixed solution and continue to culture in a constant temperature incubator for 24h.

[0189] 2.4 Treatment and feeding

[0190] After the colonization of intestinal fluid was completed, treatment feeding began. The CV group was fed with the CV diet, and the treatment group was fed with the treatment diet. Treatment feeding continued for 7 days. During this period, the growth status of the bees was observed daily and the diet was changed in time to ensure that the diet was sufficient. The culture conditions were: dark, 35℃, 60%-80% humidity.

[0191] Note: If the animal is gavage-fed individually, the diet placed in the feeding cup is CV diet, and only different treatment reagents are added to the gavage-fed diet.

[0192] 3. Experimental testing

[0193] Learning and memory test:

[0194] (1) Preparation of the behavioral observation room: Turn on the air conditioner and the exhaust fan of the test bench 1-2 hours before the experiment to keep the temperature of the observation room (26°C) constant and to ensure that there is no odor in the test bench.

[0195] (2) Bee starvation treatment: Each cup of test bees (no less than 40 bees) was starved for 2 hours in the morning.

[0196] (3) Bee tying: The starved bees are tied to a specific stand.

[0197] (4) PER (beak extension after starvation) reaction test: Use 50% sugar water to test whether the bees have PER (beak extension after starvation) reaction. If there is PER reaction, then odor learning can begin; if most of them do not, then continue to starve, and the cumulative starvation time should not exceed 3 hours. Bees without PER reaction are eliminated.

[0198] (5) Let the bees rest: The selected bees with PER response were placed in a behavior observation room (outside the test bench) for 15 minutes.

[0199] (6) Learning rate test: Place a single bee on the operating table, facing the tester, and let it stand for 10 seconds. At the 11th second, aim a 10mL empty syringe containing nonanol in front of the bee's antennae and give air at a constant speed for 6 seconds. At the 5th second of giving air, feed the bee with a cotton swab dipped in 50% sugar water. Feed for 2-3 seconds to ensure that the bee eats a small amount of sugar water. Record the bee's beak extension during the learning rate test to determine the bee's learning rate. Carry out 5 rounds of learning rate test according to the above method, with an interval of 10 minutes between each round.

[0200] (7) Memory rate test: The timer starts from the end of the fifth round of learning rate test, and the memory rate test begins 45 minutes later. The test operation is the same as the learning rate test, but each bee is tested twice, once with the odor of nonanol and once with n-hexanal, and the sugar water cotton swab is replaced with a clean cotton swab. The bee's beak extension during the memory rate test is recorded to determine the bee's memory rate for the odor.

[0201] (8) After the memory rate test is completed, the bees are tested for PER response, and the bees without PER response are regarded as invalid experimental bees.

[0202] Social behavior testing:

[0203] (1) Preparation of the behavioral observation room: Turn on the air conditioner and the exhaust fan of the test bench 1-2 hours before the experiment to keep the temperature of the observation room (26°C) constant and to ensure that there is no odor in the test bench.

[0204] (2) Place the experimental bees in groups of three in a transparent, breathable open field that has been prepared in advance.

[0205] (3) The open field was acclimated on the experimental table for 15 minutes, and the open field was filmed from above for 45 minutes.

[0206] (4) The above video was analyzed using the Etho Vision XT 17 animal behavior analysis system to obtain the social behavior ability data of honey bees.

[0207] 4. Experimental Results

[0208] (1) Results of the learning and memory experiment

[0209] Establish an animal model to simulate the individual development and neurodevelopment of infants and young children, and measure the indicators related to cognitive function. Learning and memory experiments are the most intuitive manifestation of cognitive function. Figure 1 As shown, the memory rate is Figure 2As shown. The learning rate and memory rate of Example Group 1 are significantly higher than those of the control group. As the proportion of ARA in PUFAs increases, the learning rate and memory rate of Example Group 2 and Example Group 3 decrease, but there is no significant difference from the control group. As the proportion of ARA in PUFAs decreases, the learning rate and memory rate of Example Group 4 are significantly higher than those of the control group, and there is no significant difference from Example Group 1. The results show that the addition of PUFAs can significantly improve learning and memory abilities, improve intellectual development, and effectively promote the development of the nervous system.

[0210] (2) Results of social behavior experiments

[0211] Social behavior includes social willingness and / or social ability evaluation, more specifically, tendency towards social ability and social ability evaluation. Approach frequency, physical contact frequency, cumulative physical contact time, relative movement frequency, cumulative relative movement time, and cumulative approach time can directly reflect changes in an individual's social behavior ability.

[0212] The results of the effects of PUFAs on social willingness and / or social ability are as follows Figures 2 to 8 As shown. Compared with comparative example 1, the approach frequency, approach cumulative time, body contact frequency, body contact cumulative time, relative movement frequency, and relative movement cumulative time of Example Group 1 all increased; the approach frequency, approach cumulative time, body contact frequency, and relative movement frequency of Example Group 2 increased relatively; the approach frequency, body contact frequency, and relative movement frequency of Example Group 3 increased relatively; and the approach frequency of Example Group 4 showed an increasing trend. The above results show that the addition of PUFAs can improve social willingness and / or social ability.

[0213] The effects of PUFAs and SM on social behavior Figures 9 to 14 As shown. Compared with comparative example 1, example group 5 and example group 1, the approach frequency, approach cumulative time, body contact frequency, body contact cumulative time, relative movement frequency and relative movement cumulative time of example group 6 were significantly increased, indicating that social behavior ability was significantly improved. The above results show that the nutritional composition of PUFAs and milk SM can significantly improve social behavior ability and can synergistically promote individual development and neural development.

[0214] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A pediatric formula food, wherein the pediatric formula food is an infant formula food, a toddler formula food or a children's formula food, wherein the total protein content of the pediatric formula food is 10-30 g / 100 g, the fat content is 10-35 g / 100 g, the dietary fiber content is 0-6.5 g / 100 g, and the carbohydrate content is 45-60 g / 100 g; and the pediatric formula food contains DHA, ARA and phospholipids, wherein the phospholipids are sphingomyelin and / or lecithin, wherein: The mass ratio of DHA to phospholipids is 0.5-13:1, and the mass ratio of ARA to phospholipids is 0.5-17:

1.

2. The pediatric formula food according to claim 1, wherein: The mass ratio of DHA to ARA is 0.2-5:1, preferably 0.3-3:1, and more preferably 0.5-2.5:

1.

3. The pediatric formula food according to claim 1 or 2, wherein: The mass ratio of DHA to phospholipids is 0.5-8:1, and the mass ratio of ARA to phospholipids is 0.5-10:

1.

4. The pediatric formula food according to any one of claims 1 to 3, wherein: The amount of phospholipids in food is 20-138 mg / 100 g of food dry matter.

5. The pediatric formula food according to any one of claims 1 to 4, wherein: The total amount of DHA and ARA contained in food is 100-180 mg / 100 g of food dry matter.

6. The pediatric formula food according to any one of claims 1 to 5, wherein: The dietary fiber includes one or more of galacto-oligosaccharide and fructo-oligosaccharide.

7. The pediatric formula food according to claim 1, wherein the raw materials thereof comprise:

8. Use of the pediatric formula food according to any one of claims 1 to 7 in the preparation of a product that helps to enhance and / or improve the social behavior of an individual.

9. The use according to claim 8, wherein: The said enhancing and / or improving individual social behavior includes: enhancing individual social willingness and / or social ability; Preferably, the social ability includes social interaction ability and / or social adaptability.

10. The use according to claim 8 or 9, wherein: The individual is an infant, toddler or child.

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