Penetration enhancing composition of anti-wrinkle firming active and anti-wrinkle firming cosmetic

Abstract

This application provides a penetration-enhancing composition for anti-wrinkle and firming active ingredients and an anti-wrinkle and firming cosmetic. The penetration-enhancing composition for anti-wrinkle and firming active ingredients comprises: an anti-wrinkle and firming active ingredient, including one or more of arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract; and a penetration-enhancing composition, including 1,2-pentanediol, inositol, and sodium subtilisin. This application can promote the penetration of anti-wrinkle and firming active ingredients, reduce the amount of anti-wrinkle and firming active ingredients added to cosmetics, and help reduce production costs. Furthermore, the resulting anti-wrinkle and firming cosmetic can better inhibit the expression of MMP-9, promote the expression of type I collagen, and reduce the area of ​​wrinkles.

Description

Technical Field

[0001] This application belongs to the field of cosmetic technology, specifically relating to a penetration-enhancing composition of anti-wrinkle and firming active substances and an anti-wrinkle and firming cosmetic. Background Technology

[0002] As people's living standards improve, their attention to skin is increasing daily. Cosmetics are substances applied to the skin to maintain its health. There are many types of cosmetics, which can be categorized by function, including moisturizing, acne-fighting, redness-reducing, anti-aging, whitening, and sun protection products, among others. Anti-aging products are particularly popular among middle-aged people.

[0003] Anti-wrinkle and firming are key functions of anti-aging products. Existing lifting and firming cosmetics sometimes use high proportions of macromolecular film-forming agents, fillers, or are used in conjunction with instruments or lifting and firming masks to give consumers a firming and lifting effect, but their lasting effect is poor. Others add high proportions of peptide active ingredients, but these are costly to produce and have low cost-effectiveness. Summary of the Invention

[0004] This application provides a penetration-enhancing composition for anti-wrinkle and firming active substances and an anti-wrinkle and firming cosmetic, which can promote the penetration of anti-wrinkle and firming active substances, reduce the amount of anti-wrinkle and firming active substances added to cosmetics, and help reduce production costs.

[0005] The first aspect of this application provides a penetration-enhancing composition of an anti-wrinkle and firming active substance, comprising:

[0006] Anti-wrinkle and firming active ingredients, including one or more of arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract;

[0007] The permeation-enhancing composition includes 1,2-pentanediol, inositol, and sodium subtilisin.

[0008] In a feasible embodiment of the first aspect of this application, the penetration-enhancing composition comprises 1,2-pentanediol, inositol, and sodium subtilisin in a mass ratio of (1-5):(0.2-1):1.

[0009] In a feasible embodiment of the first aspect of this application, the anti-wrinkle and firming active substances include an arginine / lysine polypeptide, tripeptide-1 copper, and sturgeon caviar extract in a mass ratio of (1-5):(0.05-1):5.

[0010] In a feasible embodiment of the first aspect of this application, the anti-wrinkle and firming active substance further includes one or more of acetyl hexapeptide-8, potassium cocoyl hydrolyzed collagen, and dipeptide diaminobutyryl benzylamide diacetate.

[0011] The second aspect of this application provides an anti-wrinkle and firming cosmetic, comprising a penetration-enhancing composition of the anti-wrinkle and firming active substances provided in the first aspect of this application.

[0012] In a feasible embodiment of the second aspect of this application, based on a total mass content of 100% for the anti-wrinkle and firming cosmetics, the anti-wrinkle and firming cosmetics include the following components in mass content:

[0013] The penetration-enhancing composition comprises 2% to 10% 1,2-pentanediol, inositol, and sodium subtilisin;

[0014] Anti-wrinkle and firming active ingredients: 5%–30%, including arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract;

[0015] Additives 5%–10% and water 65%–85%.

[0016] In a feasible embodiment of the second aspect of this application, based on a total mass content of 100% for the anti-wrinkle and firming cosmetics, the penetration-enhancing composition comprises: 1%–9% 1,2-pentanediol, 0.1%–5% inositol, and 0.1%–5% sodium subtilisin.

[0017] Optional ingredients include 1,2-pentanediol (1%–5%), inositol (0.1%–0.5%), and subtilisin sodium (0.2%–1%).

[0018] In a feasible embodiment of the second aspect of this application, based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming active ingredients include: 1% to 5% arginine / lysine polypeptide, 0.05% to 1% tripeptide-1 copper, 3% to 6% sturgeon caviar extract, 0.2% to 2% acetyl hexapeptide-8, 1% to 4% potassium cocoyl hydrolyzed collagen, and 0.2% to 2% dipeptide diaminobutyryl benzylamide diacetate.

[0019] In a feasible embodiment of the second aspect of this application, the additives include one or more of thickeners, humectants, preservatives, and pH adjusters;

[0020] Optionally, the thickener may include one or more of carbomer, xanthan gum, sodium alginate and gum arabic;

[0021] Optionally, the moisturizer includes one or more of 1,3-propanediol, glycerin, butylene glycol and dipropylene glycol;

[0022] Optionally, preservatives include one or more of p-hydroxyacetophenone, 1,2-hexanediol, phenoxyethanol, and benzyl alcohol;

[0023] Optionally, pH adjusters may include one or more of arginine, serine, and glutamic acid.

[0024] In a feasible embodiment of the second aspect of this application, based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming cosmetic comprises the following components in the following mass contents: carbomer 0.05%–1%, xanthan gum 0.05%–1%, 1,3-propanediol 2%–8%, p-hydroxyacetophenone 0.5%–2.0%, 1,2-hexanediol 0.5%–2.0%, arginine / lysine polypeptide 1%–5%, tripeptide-1 copper 0.05%–1%, sturgeon caviar extract 3%–6%, acetyl hexapeptide-8 0.2%–2%, cocoyl hydrolyzed collagen potassium 1%–4%, dipeptide diaminobutyryl benzylamide diacetate 0.2%–2%, 1,2-pentanediol 1%–5%, inositol 0.1%–0.5%, subtilisin sodium 0.2%–1%, arginine 1%–5%, and water 65%–85%. Detailed Implementation

[0025] To make the inventive objectives, technical solutions, and beneficial technical effects of this application clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the embodiments described in this specification are merely illustrative and not intended to limit the scope of this application.

[0026] For simplicity, this paper only explicitly discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form an undefined range; and any lower limit can be combined with other lower limits to form an undefined range, just as any upper limit can be combined with any other upper limit to form an undefined range. Furthermore, although not explicitly stated, every point or individual value between the endpoints of a range is included within that range. Therefore, each point or individual value can serve as its own lower or upper limit and be combined with any other point or individual value, or with other lower or upper limits, to form an undefined range.

[0027] In the description herein, when a composition is described as containing, comprising, or including a specific component, or when a process is described as containing, comprising, or including a specific process step, it is anticipated that the composition of this application is also primarily composed of or consisting of the said component, and that the process of this application is also primarily composed of or consisting of the said process step.

[0028] Unless otherwise expressly stated, the use of the terms “including,” “contains,” “comprising,” “containing,” and “having” should generally be interpreted as open-ended and non-restrictive.

[0029] In this description, it should be noted that, unless otherwise stated, "above" and "below" include the stated number, and "multiple" in "one or more" means two or more.

[0030] The foregoing description of this invention is not intended to describe every disclosed embodiment or implementation. Instead, the following description provides more specific examples of exemplary embodiments. Throughout the application, guidance is provided through a series of embodiments that can be used in various combinations. The examples listed are merely representative and should not be construed as exhaustive.

[0031] This application provides a penetration-enhancing composition for anti-wrinkle and firming active substances and an anti-wrinkle and firming cosmetic, which can promote the penetration of anti-wrinkle and firming active substances, improve their utilization rate, thereby reducing the amount of anti-wrinkle and firming active substances added to cosmetics and helping to reduce production costs.

[0032] The first aspect of this application provides a penetration-enhancing composition of an anti-wrinkle and firming active substance, comprising:

[0033] Anti-wrinkle and firming active ingredients, including one or more of arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract;

[0034] The permeation-enhancing composition includes 1,2-pentanediol, inositol, and sodium subtilisin.

[0035] The anti-wrinkle and firming active ingredients in this application include arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract, which have good water solubility. The penetration-enhancing composition of this application can soften the skin surface, reduce the resistance of the stratum corneum to the anti-wrinkle and firming active ingredients, reduce the surface tension of water, enhance the skin's hydrophilicity, and improve the osmotic pressure environment, thus facilitating the penetration of the anti-wrinkle and firming active ingredients into the skin and increasing their utilization rate. This reduces the amount of anti-wrinkle and firming active ingredients added to cosmetics, thereby lowering production costs. When applied to anti-wrinkle and firming cosmetics, it can inhibit the expression of MMP-9, promote the expression of type I collagen, and reduce the area of ​​wrinkles.

[0036] In some embodiments, the penetration-enhancing composition comprises 1,2-pentanediol, inositol, and sodium subtilisin in a mass ratio of (1-5):(0.2-1):1.

[0037] When applied to cosmetics, penetration-enhancing compounds within the above-mentioned mass ratio range can better promote the penetration of anti-wrinkle and firming active ingredients. When applied to cosmetics, they can inhibit the expression of MMP-9, promote the expression of type I collagen, and reduce the area of ​​wrinkles.

[0038] In some embodiments, the anti-wrinkle and firming active ingredients include an arginine / lysine polypeptide, tripeptide-1 copper, and sturgeon caviar extract in a mass ratio of (1–5):(0.05–1):5.

[0039] The anti-wrinkle and firming active ingredients combined with the penetration-enhancing composition within the above-mentioned mass ratio range have better effects.

[0040] In some embodiments, the anti-wrinkle and firming active ingredients also include one or more of acetyl hexapeptide-8, potassium cocoyl hydrolyzed collagen, and dipeptide diaminobutyryl benzylamide diacetate.

[0041] The second aspect of this application provides a penetration-enhancing composition comprising the anti-wrinkle and firming active substances provided in the first aspect of this application as described above.

[0042] In some embodiments, based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming cosmetic comprises the following components by mass content:

[0043] The penetration-enhancing composition comprises 2% to 10% 1,2-pentanediol, inositol, and sodium subtilisin;

[0044] Anti-wrinkle and firming active ingredients: 5%–30%, including arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract;

[0045] Additives 5%–10% and water 65%–85%.

[0046] In a feasible embodiment of the second aspect of this application, based on a total mass content of 100% for the anti-wrinkle and firming cosmetics, the penetration-enhancing composition comprises: 1%–9% 1,2-pentanediol, 0.1%–5% inositol, and 0.1%–5% sodium subtilisin.

[0047] Optional ingredients include 1,2-pentanediol (1%–5%), inositol (0.1%–0.5%), and subtilisin sodium (0.2%–1%).

[0048] In some embodiments, based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming active ingredients include: 1%–5% arginine / lysine polypeptide, 0.05%–1% tripeptide-1 copper, 3%–6% sturgeon caviar extract, 0.2%–2% acetyl hexapeptide-8, 1%–4% potassium cocoyl hydrolyzed collagen, and 0.2%–2% dipeptide diaminobutyryl benzylamide diacetate.

[0049] The anti-wrinkle and firming cosmetic product of this application can inhibit the expression of MMP-9, promote the expression of type I collagen, reduce the area of ​​wrinkles, and has low production cost and high cost performance.

[0050] In this application, arginine / lysine polypeptide, acetyl hexapeptide-8, cocoyl hydrolyzed collagen potassium, and dipeptide diaminobutyrylbenzylamide diacetate refer to the INCI names of the raw materials, which may be aqueous solutions containing these substances. The mass content of the above-mentioned substances in this application may refer to the content of the solution containing the above-mentioned substances.

[0051] For example, the components of this application, such as 1%–5% arginine / lysine polypeptide, 0.2%–2% acetyl hexapeptide-8, 1%–4% potassium cocoyl hydrolyzed collagen, and 0.2%–2% dipeptide diaminobutyryl benzylamide diacetate, can be 1%–5% arginine / lysine polypeptide solution (active substance concentration of 50 ppm), 0.2%–2% acetyl hexapeptide-8 solution (active substance concentration of 500 ppm), 1%–4% potassium cocoyl hydrolyzed collagen solution (active substance concentration of 1000 ppm), and 0.2%–2% dipeptide diaminobutyryl benzylamide diacetate solution (active substance concentration of 100 ppm).

[0052] In some embodiments, the additives include one or more of thickeners, humectants, preservatives, and pH adjusters;

[0053] Optionally, the thickener may include one or more of carbomer, xanthan gum, sodium alginate and gum arabic;

[0054] Optionally, the moisturizer includes one or more of 1,3-propanediol, glycerin, butylene glycol and dipropylene glycol;

[0055] Optionally, preservatives include one or more of p-hydroxyacetophenone, 1,2-hexanediol, phenoxyethanol, and benzyl alcohol;

[0056] Optionally, pH adjusters may include one or more of arginine, serine, and glutamic acid.

[0057] In some embodiments, based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming cosmetic comprises the following components in the following mass contents: carbomer 0.05%–1%, xanthan gum 0.05%–1%, 1,3-propanediol 2%–8%, p-hydroxyacetophenone 0.5%–2.0%, 1,2-hexanediol 0.5%–2.0%, arginine / lysine polypeptide 1%–5%, tripeptide-1 copper 0.05%–1%, sturgeon caviar extract 3%–6%, acetyl hexapeptide-8 0.2%–2%, cocoyl hydrolyzed collagen potassium 1%–4%, dipeptide diaminobutyryl benzylamide diacetate 0.2%–2%, 1,2-pentanediol 1%–5%, inositol 0.1%–0.5%, subtilisin sodium 0.2%–1%, arginine 1%–5%, and water 65%–85%.

[0058] In some embodiments, a method for preparing an anti-wrinkle and firming cosmetic includes:

[0059] A solution comprising water, carbomer, xanthan gum, 1,3-propanediol, p-hydroxyacetophenone and 1,2-hexanediol is prepared to obtain phase A.

[0060] Acetyl hexapeptide-8 (500 ppm), arginine / lysine peptide (500 ppm), cocoyl hydrolyzed collagen potassium (1000 ppm), tripeptide-1 copper, dipeptide diaminobutyryl benzylamide diacetate (100 ppm), and sturgeon caviar extract were mixed to obtain phase B solution.

[0061] 1,2-Pentanediol, inositol, subtilisin sodium, and arginine were mixed to obtain phase C solution;

[0062] The A-phase solution, B-phase solution, and C-phase solution are mixed and stirred to obtain an anti-wrinkle and firming cosmetic.

[0063] Example

[0064] The following embodiments describe the disclosure of this application in more detail. These embodiments are merely illustrative, as various modifications and variations will be apparent to those skilled in the art within the scope of the disclosure of this application. Unless otherwise stated, all parts, percentages, and ratios reported in the following embodiments are based on weight, and all reagents used in the embodiments are commercially available or synthesized by conventional methods and can be used directly without further processing, and the instruments used in the embodiments are commercially available.

[0065] Example 1

[0066] A wrinkle-reducing and firming cosmetic comprises the following components in weight percentages: Phase A: Water 78.9%, Carbomer 0.1%, Xanthan Gum 0.1%, 1,3-Propanediol 5%, p-Hydroxyacetophenone 0.5%, 1,2-Hexanediol 0.5%; Phase B: Acetyl Hexapeptide-8 (500ppm) 1%, Arginine / Lysine Peptide (500ppm) 2%, Cocoyl Hydrolyzed Collagen Potassium (1000ppm) 3%, Tripeptide-1 Copper 0.1%, Dipeptide Diaminobutyrylbenzylamide Diacetate (100ppm) 1%, Sturgeon Caviar Extract 5%; Phase C: 1,2-Pentanediol 2%, Inositol 0.2%, Subtilisin Sodium 0.5%, Arginine 0.1%.

[0067] A method for preparing an anti-wrinkle and firming cosmetic, comprising:

[0068] A solution comprising water, carbomer, xanthan gum, 1,3-propanediol, p-hydroxyacetophenone and 1,2-hexanediol is prepared to obtain phase A.

[0069] Acetyl hexapeptide-8 (500 ppm), arginine / lysine peptide (500 ppm), cocoyl hydrolyzed collagen potassium (1000 ppm), tripeptide-1 copper, dipeptide diaminobutyryl benzylamide diacetate (100 ppm) and sturgeon caviar extract were mixed to obtain phase B solution;

[0070] 1,2-Pentanediol, inositol, subtilisin sodium, and arginine were mixed to obtain phase C solution;

[0071] The A-phase solution, B-phase solution, and C-phase solution are mixed and stirred to obtain an anti-wrinkle and firming cosmetic.

[0072] Examples 2-16

[0073] Compared with Example 1, Examples 2-7 differ in that the penetration-enhancing composition has been changed, and the specific parameters of the change are shown in Table 1.

[0074] Compared with Example 1, Examples 8-16 differ in that the anti-wrinkle and firming active ingredients have changed, and the specific parameters of the changes are shown in Table 1.

[0075] Comparative Examples 1-6

[0076] Comparative Examples 1-3 differ from Example 1 in that only two of the two components were added to the permeation-enhancing composition. The specific parameters of the changes are shown in Table 1.

[0077] Comparative Examples 4-6 differ from Example 1 in that the types of penetration-enhancing compositions were substituted, as shown in Table 1.

[0078] Test section

[0079] Thirty volunteers were recruited, and the product was applied to their skin. After data processing, the MMP-9 inhibition rate, type I collagen expression level, and wrinkle area were tested.

[0080] Matrix metalloproteinase (MMP-9) activity assay

[0081] A 3D skin model was constructed to simulate skin structure. HDF-α cells were stimulated with UVA, and then equal amounts of the example and comparative samples were added to different culture dishes. One dish was left untreated as a blank control group. After the same amount of time, the expression level of MMP-9 (matrix metalloproteinase) mRNA was detected. The results are shown in Table 2, with some plotted as bar charts.

[0082] Dermal collagen expression test

[0083] A 3D skin model was constructed to simulate skin structure. HDF-α cells were stimulated with UVA. Equal amounts of the examples and comparative samples were then added to different culture dishes. A blank control group was not irradiated with UVA and no sample was added. After the same incubation period, the expression level of COL1 (type I collagen) mRNA in dermal fibroblasts damaged by UVA was detected in the culture dishes. The results are shown in Table 2, with some plotted as bar charts.

[0084] Wrinkle area change test

[0085] One hundred volunteers were recruited, and the various examples and comparative samples were applied to the skin of the facial wrinkle area to test the area of ​​wrinkles at three time points: before use (D0), 14 days after use (D14), and 28 days after use (D28).

[0086] Table 1:

[0087]

[0088]

[0089] Table 2:

[0090]

[0091] As can be seen from the table, the embodiments of this application can significantly inhibit the expression level of MMP-9 (matrix metalloproteinase) mRNA in dermal fibroblasts damaged by UVA, and promote the expression level of COL1 (type I collagen) mRNA in dermal fibroblasts damaged by UVA, thereby reducing the area of ​​wrinkles. This demonstrates that the composition of this application can promote the penetration of anti-wrinkle and firming active substances, improving the anti-wrinkle effect of anti-wrinkle and firming cosmetics. In particular, Example 1, with its rationally configured penetration-enhancing composition and anti-wrinkle and firming composition and ratio, shows even better improvement and a superior cost-performance ratio.

[0092] The above embodiments of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the implementation of the present invention. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively describe all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A penetration-enhancing composition of an anti-wrinkle and firming active ingredient, characterized in that, The anti-wrinkle and firming active ingredient penetration-enhancing composition includes: Anti-wrinkle and firming active ingredients include arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract in a mass ratio of (1~5):(0.05~1):5; The penetration-enhancing composition comprises 1,2-pentanediol, inositol, and sodium subtilisin in a mass ratio of (1-5):(0.2-1):

1.

2. The penetration-enhancing composition of the anti-wrinkle and firming active substance according to claim 1, characterized in that, The anti-wrinkle and firming active ingredients also include one or more of acetyl hexapeptide-8, potassium cocoyl hydrolyzed collagen, and dipeptide diaminobutyryl benzylamide diacetate.

3. A cosmetic product for anti-wrinkle and firming, characterized in that, A penetration-enhancing composition comprising the anti-wrinkle and firming active substance as described in any one of claims 1 to 2.

4. The anti-wrinkle and firming cosmetic according to claim 3, characterized in that, Based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming cosmetic comprises the following components by mass content: The permeation-enhancing composition comprises 2% to 10% of 1,2-pentanediol, inositol, and sodium subtilisin. The anti-wrinkle and firming active ingredients, comprising 5% to 30% of arginine / lysine peptides, tripeptide-1 copper, and sturgeon caviar extract; Additives 5%~10% and water 65%~85%.

5. The anti-wrinkle and firming cosmetic according to claim 3, characterized in that, Based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the penetration-enhancing composition comprises: 1% to 9% 1,2-pentanediol, 0.1% to 5% inositol, and 0.1% to 5% sodium subtilisin.

6. The anti-wrinkle and firming cosmetic according to claim 5, characterized in that, The composition includes 1%~5% 1,2-pentanediol, 0.1%~0.5% inositol, and 0.2%~1% sodium subtilisin.

7. The anti-wrinkle and firming cosmetic according to claim 3, characterized in that, Based on a total mass content of 100% for the anti-wrinkle and firming cosmetics, the anti-wrinkle and firming active ingredients include: 1%~5% arginine / lysine polypeptide, 0.05%~1% tripeptide-1 copper, 3%~6% sturgeon caviar extract, 0.2%~2% acetyl hexapeptide-8, 1%~4% potassium cocoyl hydrolyzed collagen, and 0.2%~2% dipeptide diaminobutyryl benzylamide diacetate.

8. The anti-wrinkle and firming cosmetic according to claim 4, characterized in that, The additives include one or more of the following: thickeners, humectants, preservatives, and pH adjusters.

9. The anti-wrinkle and firming cosmetic according to claim 8, characterized in that, The thickener includes one or more of carbomer, xanthan gum, sodium alginate, and gum arabic.

10. The anti-wrinkle and firming cosmetic according to claim 8, characterized in that, The moisturizer includes one or more of 1,3-propanediol, glycerin, butylene glycol, and dipropylene glycol.

11. The anti-wrinkle and firming cosmetic according to claim 8, characterized in that, The preservatives include one or more of p-hydroxyacetophenone, 1,2-hexanediol, phenoxyethanol, and benzyl alcohol.

12. The anti-wrinkle and firming cosmetic according to claim 8, characterized in that, The pH adjuster includes one or more of arginine, serine, and glutamic acid.

13. The anti-wrinkle and firming cosmetic according to claim 3, characterized in that, Based on a total mass content of 100% for the anti-wrinkle and firming cosmetic, the anti-wrinkle and firming cosmetic comprises the following components in the following mass contents: carbomer 0.05%~1%, xanthan gum 0.05%~1%, 1,3-propanediol 2%~8%, p-hydroxyacetophenone 0.5%~2.0%, 1,2-hexanediol 0.5%~2.0%, arginine / lysine peptide 1%~5%, tripeptide-1 copper 0.05%~1%, sturgeon caviar extract 3%~6%, acetyl hexapeptide-8 0.2%~2%, cocoyl hydrolyzed collagen potassium 1%~4%, dipeptide diaminobutyryl benzylamide diacetate 0.2%~2%, 1,2-pentanediol 1%~5%, inositol 0.1%~0.5%, subtilisin sodium 0.2%~1%, arginine 1%~5%, and water 65%~85%.

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