Hospital sewage disinfection process for intelligent targeted virus disinfection

Abstract

The invention relates to the field of sewage treatment, and discloses an intelligent targeted virus disinfection hospital sewage disinfection process which comprises the following steps: S1, preparing ozone micro-nano bubbles through a micro-nano bubble generator, and injecting ozone gas into sewage to form micro-nano bubbles; s2, adding an oxidizing agent into the sewage containing the ozone micro-nano bubbles to destroy lipid envelopes of the viruses; s3, degrading a protein shell of the virus through a synergistic effect of hydroxyl free radicals and a chemical modifier; s4, destroying viral nucleic acid by combining ultraviolet irradiation and a catalyst; s5, controlling generation of by-products in the reaction process; and S6, after water outlet detection is carried out, water drainage is completed. Through multi-layer structure staged treatment of intelligent targeting viruses, lipid envelope destruction, protein shell degradation and nucleic acid thorough destruction are combined with synergistically optimized oxidation, modification and degradation mechanisms, so that the virus killing efficiency is remarkably improved, and the treatment comprehensiveness and accuracy are ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to a hospital sewage disinfection process for intelligent targeted virus elimination. Background Art

[0002] Virus contamination is one of the most important safety hazards in hospital sewage. Viruses have complex structures and strong environmental adaptability, usually consisting of an outer lipid envelope, a protein shell, and an internal nucleic acid. Traditional sewage disinfection technologies such as single ozone disinfection, ultraviolet sterilization, or chemical oxidant treatment are usually unable to completely destroy the multi-layer structure of the virus, and are prone to incomplete disinfection. In addition, when the virus is not completely inactivated, it may form highly resistant infectious residues, increasing the risk of secondary transmission in the water.

[0003] Existing technologies mainly focus on the application of a single disinfection method. For example, ozone disinfection can effectively destroy the viral envelope, but its solubility in water is poor and it is easy to decompose, resulting in limited sterilization efficiency; ultraviolet disinfection can directly act on viral nucleic acids, but the treatment effect on protein shells and envelopes is limited; chemical oxidants such as hypochlorite have good performance in virus disinfection, but may generate toxic byproducts such as bromate and chlorate, affecting water quality safety. These methods lack specificity in actual use, cannot be graded according to the different structures of the virus, and are difficult to achieve intelligent dynamic regulation.

[0004] In addition, existing technologies often fail to fully inactivate the residual nucleic acid and protein shell of the virus in the subsequent stages of sewage treatment, resulting in some inactivated virus fragments still being infectious, increasing environmental safety risks. At the same time, many traditional methods lack dynamic control mechanisms and cannot adjust process parameters in real time according to the virus load in sewage or changes in water quality, resulting in waste of resources and large fluctuations in treatment effects. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a hospital sewage disinfection process with intelligent targeted virus disinfection, which solves the problems in the prior art that the virus disinfection process cannot efficiently target the multi-layer structure of the virus, lacks dynamic optimization control, and the generation of by-products is difficult to effectively inhibit.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a hospital sewage disinfection process with intelligent targeted virus disinfecting, comprising the following steps:

[0007] S1: Prepare ozone micro-nano bubbles through a micro-nano bubble generator, and inject ozone gas into sewage to form micro-nano bubbles;

[0008] S2: Adding oxidants to wastewater containing ozone micro-nano bubbles to destroy the lipid envelope of the virus;

[0009] S3: Adjust the pH value of sewage and reaction time to degrade the protein shell of the virus through the synergistic effect of hydroxyl radicals and chemical modifiers;

[0010] S4: Combining UV irradiation with a catalyst to destroy viral nucleic acids;

[0011] S5: Control the generation of by-products during the reaction and inactivate residual viral components by adding enzymes;

[0012] S6: After the water outlet detection is performed, the drainage is completed.

[0013] Preferably, the S1 step comprises:

[0014] S11: Generate ozone gas using an ozone generator, the concentration of which is controlled at 50-100 mg / L, and introduce it into a gas-liquid mixing system through a gas pipeline;

[0015] S12: mixing the ozone gas with the sewage, and utilizing the turbulence effect to disperse the ozone gas into micro-nano bubbles with a diameter of 50-200 nm during the mixing process;

[0016] S13: Distribute micro-nano bubbles in the sewage to control the concentration of ozone dissolved in the bubbles to 10-20ppm.

[0017] Preferably, the S2 step comprises:

[0018] S21: preparing an oxidant solution, wherein the oxidant is a natural polyphenol compound;

[0019] S22: slowly adding the prepared oxidant solution into the sewage in batches according to the viral load;

[0020] S23: Oxidation of fatty acid molecules in the viral lipid envelope through the interfacial effect of ozone micro-nanobubbles;

[0021] S24: The oxidation reaction is maintained under the condition that the sewage temperature is controlled at 25-30°C for a duration of 10-15 minutes.

[0022] Preferably, the S3 step comprises:

[0023] S31: adjusting the pH value of the sewage to 6.5-7.5 by adding an acid or alkaline agent;

[0024] S32: maintaining the reaction temperature at 25-30°C and controlling the reaction time to 10-20 min;

[0025] S33: introducing a chemical modifier into the sewage, the chemical modifier including but not limited to guanidinoacetic acid or urea, with a concentration of 5-10 mg / L;

[0026] S34: Ozonolysis and oxidant action synergistically generate hydroxyl radicals, which react with key amino acids in the viral protein coat.

[0027] Preferably, the S4 step comprises:

[0028] S41: Adjust the pH value of sewage to maintain at 6.5-7.5;

[0029] S42: UV irradiation of wastewater to directly destroy the phosphodiester bonds of viral nucleic acids;

[0030] S43: adding a catalyst to the sewage while irradiating with ultraviolet light;

[0031] S44: Generates hydroxyl radicals, superoxide ions and elemental oxygen through catalytic reactions.

[0032] Preferably, the step S5 comprises:

[0033] S51: adding one or more of nitrate and nitrite to the sewage as competitive oxidants, with the dosage controlled at 1-3 mg / L;

[0034] S52: Adjust the pH value of sewage to 6.5-7.5 to inhibit chlorate formation;

[0035] S53: After the reaction is completed, RNase is added to inactivate the residual viral nucleic acid and viral protein shell in the sewage. The enzyme addition concentration is controlled at 5-10 mg / L;

[0036] S54: The temperature of the enzyme inactivation reaction is maintained at 25-30°C, and the reaction time is 10 minutes.

[0037] Preferably, the step S6 comprises:

[0038] S61: Detect the concentration of viral RNA or DNA in sewage by real-time fluorescence quantitative PCR;

[0039] S62: Testing the pH value, dissolved oxygen concentration and turbidity of the effluent;

[0040] S63: When the detection index does not meet the standard, the steps S2-S5 are cycled;

[0041] S64: When the test indicators meet the standards, the sewage will be discharged into the municipal drainage system or reused in non-drinking water treatment scenarios.

[0042] Preferably, the concentration of the oxidant solution in S21 is 10-30 mg / L, and the oxidant solution includes but is not limited to catechol or gallic acid.

[0043] Preferably, in step S42, the wavelength of ultraviolet light is set to 180-254 nm, and the irradiation intensity is controlled at 20-40 mJ / cm 2 The ultraviolet action time is 5-10 minutes.

[0044] Preferably, the catalyst in step S43 includes but is not limited to Fe 2+ , Mn 2+ Or TiO2, ZnO, the concentration of the catalyst is controlled at 1-3 mg / L.

[0045] The present invention provides a hospital sewage disinfection process with intelligent targeted virus elimination, which has the following beneficial effects:

[0046] 1. The present invention uses intelligent targeted multi-layer structure hierarchical treatment of viruses, from lipid envelope destruction to protein shell degradation to complete nucleic acid destruction, combined with synergistically optimized oxidation, modification and degradation mechanisms, to significantly improve the virus killing efficiency and ensure the comprehensiveness and accuracy of the treatment.

[0047] 2. The present invention utilizes competitive oxidants to precisely inhibit the generation of by-products, and combines enzyme substances to target and inactivate residual viral nucleic acids and protein components, thereby ensuring that viruses in sewage are completely inactivated and achieving efficient and environmentally friendly discharge.

[0048] 3. The present invention combines an intelligent dynamic optimization control mechanism to adjust reaction conditions such as pH value, temperature and agent concentration in real time, and optimizes multi-target treatment efficiency based on viral load to ensure that the entire disinfection process is efficient and stable and adaptable to complex sewage treatment scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is a schematic diagram of the overall process steps of the present invention;

[0050] Figure 2 This is a detailed flow chart of step S1 of the present invention;

[0051] Figure 3 This is a detailed flow diagram of step S2 of the present invention;

[0052] Figure 4 It is a detailed flow diagram of step S3 of the present invention;

[0053] Figure 5 This is a detailed flow diagram of step S4 of the present invention;

[0054] Figure 6 It is a detailed flow diagram of step S5 of the present invention;

[0055] Figure 7 Detailed flow diagram of step S6 of the present invention. DETAILED DESCRIPTION

[0056] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0057] Example:

[0058] Please see attached Figure 1 The embodiment of the present invention provides a hospital sewage disinfection process for intelligent targeted virus disinfecting, comprising the following steps:

[0059] Please see attached Figure 2 , S1: preparing ozone micro-nano bubbles through a micro-nano bubble generator, and injecting ozone gas into sewage to form micro-nano bubbles;

[0060] S11: Generate ozone gas using an ozone generator, the concentration of which is controlled at 50-100 mg / L, and introduce it into a gas-liquid mixing system through a gas pipeline;

[0061] S12: mixing the ozone gas with the sewage, and utilizing the turbulence effect to disperse the ozone gas into micro-nano bubbles with a diameter of 50-200 nm during the mixing process;

[0062] S13: Distribute micro-nano bubbles in the sewage to control the concentration of ozone dissolved in the bubbles to 10-20ppm.

[0063] Specifically, the generation of ozone gas is the basic link of this step. Ozone gas is generated by high-voltage discharge or electrolysis using oxygen or air as a gas source through an ozone generator. In actual operation, in order to ensure the generation efficiency and concentration stability of ozone, it is preferred to use a high-voltage discharge method and use oxygen as a raw gas source to avoid the influence of impurities in the air on the ozone concentration. The ozone generator needs to be set in an area close to the gas-liquid mixing system, and the ozone generation concentration is controlled within the range of 50-100 mg / L to meet the needs of the subsequent gas-liquid mixing process. Ozone gas is introduced into the gas-liquid mixing system through a gas pipeline, and a check valve and a gas flow meter are set in the pipeline to prevent liquid backflow and ensure the flow stability of ozone gas.

[0064] The mixing process of ozone gas and sewage is the core link in the preparation of micro-nano bubbles, which directly affects the mass transfer efficiency and bubble stability of ozone. When injecting ozone gas into sewage, a venturi mixer or a high-efficiency turbulent gas-liquid mixing device can be used to fully mix the gas and liquid through the turbulent effect. The venturi tube uses the negative pressure generated by the high-speed flowing liquid in the pipeline to suck the ozone gas into the mixing system, and disperses the gas into micro-nano bubbles with a diameter of 50-200nm through turbulent shearing. If a turbulent gas-liquid mixing device is used, it is necessary to increase the liquid flow rate or introduce high shear force to achieve rapid breakage and microbubble formation of the gas-liquid interface. In order to improve the uniformity of the bubbles, a fine-pore diffuser can be installed at the outlet of the mixer to further disperse the mixed gas into smaller bubbles.

[0065] The micro-nano bubbles generated after mixing need to be evenly distributed in the sewage treatment system to ensure sufficient contact between ozone and viruses in sewage. By optimizing the design of the gas-liquid mixing system, the risk of bubble aggregation and overflow can be reduced, and the solubility of ozone and bubble stability can be improved. In specific operations, the uniform distribution of bubbles can be ensured by adjusting the gas-liquid ratio (such as 1:5) and controlling the sewage flow rate, while avoiding premature escape of bubbles. The dissolved concentration of ozone in the bubbles is monitored in real time by a dissolved ozone concentration measuring device to ensure that the dissolved ozone concentration in the sewage is maintained within the range of 10-20ppm. Within this range, the oxidation effect of ozone and the virus killing efficiency can achieve the best results. In order to further improve the mass transfer efficiency, a multi-stage gas-liquid mixing device can be selected or the residence time of bubbles in sewage can be extended to make full use of the oxidation capacity of ozone.

[0066] This step is based on the high interfacial effect and high dissolution efficiency of ozone micro-nano bubbles, which enables ozone to quickly dissolve and disperse in sewage and directly contact and react with viruses. Micro-nano bubbles can significantly improve the gas mass transfer efficiency and enhance the decomposition and oxidation capacity of ozone in the liquid phase due to their high surface area to volume ratio. In addition, micro-nano bubbles will release local high energy and active oxygen species (such as hydroxyl radicals) when they burst, further enhancing the virus disinfecting effect.

[0067] Please see attached Figure 3 , S2: adding oxidants to wastewater containing ozone micro-nano bubbles to destroy the lipid envelope of the virus;

[0068] S21: preparing an oxidant solution, wherein the oxidant is a natural polyphenol compound;

[0069] S22: slowly adding the prepared oxidant solution into the sewage in batches according to the viral load;

[0070] S23: Oxidation of fatty acid molecules in the viral lipid envelope through the interfacial effect of ozone micro-nanobubbles;

[0071] S24: The oxidation reaction is maintained under the condition that the sewage temperature is controlled at 25-30°C for a duration of 10-15 minutes.

[0072] Specifically, when implementing the present invention, the preferred oxidant is a natural polyphenol compound, such as catechol or gallic acid, which has good biodegradability and efficient oxidation ability. First, the natural polyphenol compound is dissolved in pure water or treated sewage according to the set concentration (10-30 mg / L) to prepare a uniform oxidant solution. The natural polyphenol compound can form an oxidation reaction with the fatty acid in the viral lipid envelope through the phenolic hydroxyl group in its molecule, effectively weakening the structural stability of the viral membrane. In addition, the selection of natural polyphenol oxidants can also reduce the risk of environmental pollution and avoid secondary harm to the sewage ecosystem. The oxidant solution should be used immediately after preparation to prevent the solution from being exposed for a long time and causing the oxidant activity to decrease.

[0073] The addition process of the oxidant solution is crucial to the reaction effect. In actual operation, the addition rate and number of batches of the oxidant solution are adjusted according to the virus load in the sewage and water quality conditions (such as turbidity and organic matter concentration). In order to ensure the uniform distribution of the oxidant, a slow addition method is adopted in batches, and the amount of each addition is controlled at 10-20% of the total amount, and all additions are completed at intervals of several minutes. The slow addition of the oxidant can avoid the situation where the local concentration is too high or too low, and ensure that the oxidant molecules can fully contact the viral lipid envelope. At the same time, the slow addition method forms a synergy with the continuous action of ozone micro-nano bubbles to further enhance the oxidation efficiency.

[0074] When the oxidant solution interacts with the ozone micro-nano bubbles in the sewage, a strong oxidizing environment can be formed on the surface of the viral lipid envelope by utilizing the high surface area effect and interface effect of the micro-nano bubbles. The high concentration of ozone released by the micro-nano bubbles at the interface works together with the oxidant to generate reactive oxygen species with strong oxidizing properties (such as hydroxyl radicals), which can quickly attack the fatty acid molecules in the viral lipid envelope, causing them to undergo oxidative degradation. Fatty acid oxidation destroys the fluidity and integrity of the lipid bilayer, weakens the outer membrane structure of the virus, or even completely cleaves it, thereby losing its function of protecting nucleic acids. The interface effect can also increase the rate of the oxidation reaction, significantly improving the degradation efficiency of the viral lipid envelope.

[0075] In order to ensure the efficient oxidation reaction, the reaction temperature of the sewage needs to be controlled within the range of 25-30°C. Within this temperature range, the oxidation activity and ozone decomposition rate of natural polyphenol compounds are at a high level, which can produce sufficient reactive oxygen species while avoiding the adverse effects of high temperature on the activity of the oxidant. Under the joint action of the oxidant and ozone micro-nano bubbles, the degradation of the viral lipid envelope can usually be completed within 10-15 minutes. The reaction time needs to be adjusted appropriately according to the viral load and the concentration of other organic matter in the sewage to ensure that the viral lipid envelope is fully destroyed. By regularly sampling and detecting the degree of lipid envelope degradation, it is possible to determine whether the reaction is complete and terminate the oxidation treatment in a timely manner according to the reaction situation.

[0076] The principle of this step is the synergistic effect of ozone micro-nano bubbles and natural polyphenol oxidants, which destroy the stability of the viral lipid envelope through efficient oxidation reactions. Ozone micro-nano bubbles can provide a large area of ​​gas-liquid interface, while releasing reactive oxygen species, strengthening the attack of oxidants on the viral membrane; natural polyphenol compounds react with fatty acids through phenolic hydroxyl groups in the molecules, further weakening the fluidity and stability of the membrane.

[0077] Please refer to the attached Figure 4 , S3: Adjust the pH value of sewage and reaction time to degrade the protein shell of the virus through the synergistic effect of hydroxyl radicals and chemical modifiers;

[0078] S31: adjusting the pH value of the sewage to 6.5-7.5 by adding an acid or alkaline agent;

[0079] S32: maintaining the reaction temperature at 25-30°C and controlling the reaction time to 10-20 min;

[0080] S33: introducing a chemical modifier into the sewage, the chemical modifier including but not limited to guanidinoacetic acid or urea, with a concentration of 5-10 mg / L;

[0081] S34: Ozonolysis and oxidant action synergistically generate hydroxyl radicals, which react with key amino acids in the viral protein coat.

[0082] Specifically, first of all, the pH value of the sewage needs to be adjusted to ensure the best reaction environment for the generation of hydroxyl radicals. The specific operation is to control the pH value of the sewage within the range of 6.5-7.5 by slowly adding an appropriate amount of acid (such as dilute sulfuric acid) or alkali (such as sodium hydroxide solution). Within this range, the decomposition rate of ozone in water is relatively fast, and hydroxyl radicals can be generated efficiently while maintaining the activity of chemical modifiers. The pH value of the reaction system is detected in real time by an online pH monitoring device, and fine-tuned when necessary to ensure that the entire oxidation process is always carried out under the optimal pH conditions, thereby providing the best chemical environment for the degradation reaction of the viral protein coat.

[0083] In order to enhance the activity of hydroxyl radicals and avoid damage to the reaction system by excessively high temperatures, the reaction temperature needs to be maintained at 25-30°C. Within this temperature range, hydroxyl radicals have a high oxidizing ability and can quickly react with amino acids in the viral protein shell to oxidize, while avoiding the rapid consumption of hydroxyl radicals under high temperature conditions. The reaction time needs to be dynamically adjusted according to the viral load and sewage water quality conditions, and is generally maintained at 10-20 minutes. Within this time, the full degradation of the viral shell protein can be guaranteed, and excessive oxidation can be avoided to reduce the treatment efficiency or increase the generation of by-products.

[0084] The addition of chemical modifiers is a key step in enhancing the degradation efficiency of viral protein shells. The chemical modifier is preferably guanidinoacetic acid or urea, and the concentration is controlled at 5-10 mg / L. Guanidinoacetic acid can form a chemical bond with polar amino acids (such as cysteine ​​and tyrosine) in the viral protein shell, exposing more reaction sites to the protein molecular structure, which is convenient for further attack by hydroxyl radicals; urea weakens the hydrogen bonds and hydrophobic effects of the viral shell protein, loosens and denatures its spatial structure, and significantly reduces the stability of the protein shell. When adding chemical modifiers, ensure that they are fully mixed with sewage, usually by uniform and slow addition, to prevent excessive local concentration from affecting the reaction balance.

[0085] Under the above conditions, highly active hydroxyl radicals are generated through the synergistic effect of ozone decomposition reaction and oxidant. Ozone generates hydroxyl radicals in water through the following reactions:

[0086] O3+H2O→2·OH+O2

[0087] These free radicals can react strongly with key amino acid residues in the viral protein shell (such as the sulfhydryl group of cysteine ​​and the phenolic hydroxyl group of tyrosine), destroying the side chain functional groups of the amino acids, leading to the breakage of the protein molecular chain and the instability of the shell structure. In addition, hydroxyl free radicals can further destroy the main chain of the viral shell protein by cutting peptide bonds, causing it to completely lose its functionality and structural stability.

[0088] Through the synergistic effect of ozone and oxidants, the generation efficiency of hydroxyl radicals is significantly improved. Combined with the effect of chemical modifiers, the range of reaction sites of the viral protein shell can be effectively expanded, accelerating its degradation process. In the experiment, the concentration changes of protein degradation products (such as peptides or amino acid residues) can be dynamically detected to determine the degree of completion of the reaction, and the generation rate of hydroxyl radicals can be adjusted to optimize the degradation effect when necessary.

[0089] The principle of this step is to promote the generation of hydroxyl radicals by adjusting the chemical environment of sewage, and to target and destroy the key structure of the virus shell protein through the synergistic effect of chemical modifiers. Hydroxyl radicals are extremely oxidizing and can effectively oxidize the functional amino acid residues in the virus protein shell, causing it to lose its normal chemical properties; chemical modifiers further accelerate the degradation process of the shell by exposing more reaction sites and weakening the interaction between protein molecules.

[0090] Please refer to the attached Figure 5 , S4: Combining UV irradiation with catalysts to destroy viral nucleic acids;

[0091] S41: Adjust the pH value of sewage to maintain at 6.5-7.5;

[0092] S42: UV irradiation of wastewater to directly destroy the phosphodiester bonds of viral nucleic acids;

[0093] S43: adding a catalyst to the sewage while irradiating with ultraviolet light;

[0094] S44: Generates hydroxyl radicals, superoxide ions and elemental oxygen through catalytic reactions.

[0095] Specifically, the goal of this step is to completely inactivate the virus by using reactive oxygen species (such as hydroxyl radicals, superoxide ions, and elemental oxygen) to target and destroy the phosphodiester bonds and base structures of viral nucleic acids through the synergistic effect of ultraviolet irradiation and catalysts. The specific operation method is as follows:

[0096] The pH value of sewage has an important influence on the activity of ultraviolet irradiation and catalyst reaction. In this step, the pH value of sewage is adjusted to between 6.5 and 7.5 by adding an acid (such as dilute sulfuric acid) or an alkali (such as sodium hydroxide solution). Within this pH range, the activity of the catalyst is in the best state, and the effect of ultraviolet rays and the generation efficiency of hydroxyl radicals are high. pH adjustment needs to be completed before the start of ultraviolet treatment, and the stability of pH value during the reaction process is ensured by a real-time monitoring system to avoid deviation from the set range and affect the treatment effect.

[0097] Ultraviolet irradiation is one of the core operations of this step. Its main function is to directly destroy the phosphodiester bonds in the viral nucleic acid, thereby causing the nucleic acid chain to break and lose genetic function. In the specific operation, the ultraviolet wavelength is set to 254nm, which is the maximum absorption wavelength of DNA and RNA and can effectively destroy the chemical bonds of nucleic acids. The intensity of ultraviolet irradiation is controlled at 20-40mJ / cm 2The irradiation time is 5-10 minutes, and the time can be adjusted appropriately according to the viral load in the sewage. During the irradiation process, the energy of ultraviolet rays will directly act on the bases in the nucleic acid chain (such as adenine and thymine), triggering the formation or cleavage of covalent bonds between bases, and inactivating the viral nucleic acid function.

[0098] During the ultraviolet irradiation process, a catalyst is added to the wastewater to enhance the generation efficiency of active oxygen species. The catalyst is preferably a transition metal ion (such as Fe 2+ or Mn 2+ ) or photocatalytic nanomaterials (such as TiO2 or ZnO), the concentration of which is controlled at 1-3 mg / L. When adding the catalyst, ensure that it is fully mixed with the sewage so that the catalyst is evenly distributed in the entire reaction system to maximize the synergy of ultraviolet excitation and oxidation reaction. The catalyst is excited under ultraviolet irradiation to generate electron-hole pairs (e - / h + ), electrons react with dissolved oxygen to generate superoxide ions, and holes react with water molecules to generate hydroxyl radicals, thus forming a highly active oxidative environment in the sewage.

[0099] Under the action of ultraviolet light and catalyst, reactive oxygen species are generated through the following chemical reactions:

[0100] Hydroxyl radical (·OH):

[0101] H2O+h + → OH+H +

[0102] Superoxide ion (O2-):

[0103] O2+e - →O2 -

[0104] Elemental oxygen (O):

[0105] O3→O+O2

[0106] These reactive oxygen species can rapidly react with viral nucleic acids to oxidize them, targeting and destroying the phosphodiester bonds and base structures of nucleic acids. Hydroxyl radicals break the backbone of nucleic acid chains with extremely strong oxidizing properties, superoxide ions further oxidize bases through electron transfer, and elemental oxygen further destroys the chemical stability of nucleic acids by oxidizing sugar groups in nucleic acids. These synergistic effects ultimately lead to the complete inactivation of viral nucleic acids.

[0107] To ensure the efficient progress of the catalytic reaction, the concentration of active oxygen species in the reaction system can be monitored, and the ultraviolet irradiation intensity and catalyst concentration can be adjusted in time according to the viral load or the degree of nucleic acid degradation in the sewage.

[0108] Please see attached Figure 6 , S5: Control the generation of by-products during the reaction and inactivate the residual virus components by adding enzyme substances;

[0109] S51: adding one or more of nitrate and nitrite to the sewage as competitive oxidants, with the dosage controlled at 1-3 mg / L;

[0110] S52: Adjust the pH value of sewage to 6.5-7.5 to inhibit chlorate formation;

[0111] S53: After the reaction is completed, RNase is added to inactivate the residual viral nucleic acid and viral protein shell in the sewage. The enzyme addition concentration is controlled at 5-10 mg / L;

[0112] S54: The temperature of the enzyme inactivation reaction is maintained at 25-30°C, and the reaction time is 10 minutes.

[0113] Specifically, the goal of this step is to control the byproducts that may be generated during the reaction by using competitive oxidants, and at the same time use enzymes to completely passivate the residual viral nucleic acids and viral protein shells in the sewage to achieve safe treatment and discharge of sewage. The specific operation method is as follows:

[0114] During ozone disinfection and oxidation reactions, bromide ions (Br - ) easily reacts with ozone to form bromate (BrO3 - ), and bromate is a byproduct that is harmful to the environment and human body. In order to inhibit the formation of bromate, nitrate (NO3 - ) or nitrite (NO2 - ) as a competitive oxidant. Nitrates and nitrites can react with ozone preferentially, reducing the contact opportunities between ozone and bromide ions, thereby effectively reducing the amount of bromate generated. In specific operations, nitrates or nitrites are prepared into a solution of appropriate concentration, and slowly added to the sewage at a dosage of 1-3 mg / L. At the same time, the concentration of bromate is detected in real time through an online monitoring system to ensure that the concentration of by-products generated is lower than the international emission standards (for example, bromate is lower than 10 μg / L). The implementation of this step not only improves the safety of the reaction, but also enhances the environmental performance of sewage treatment.

[0115] During the ozone oxidation process, chloride ions (Cl - ) may react with ozone to form chlorate (ClO3 -), this by-product also has potential hazards to water bodies and the ecological environment. Studies have shown that the formation of chlorate is significantly affected by the pH value. By adding an acid or alkaline agent and adjusting the pH value of the sewage to the range of 6.5-7.5, the formation of chlorate can be significantly inhibited, while ensuring the optimal reaction efficiency of ozone and oxidants. In specific operations, dilute sulfuric acid or sodium hydroxide solution can be used for adjustment. During the adjustment process, the pH value of the sewage needs to be monitored in real time and its stability maintained. Within this range, not only can the formation of chlorate be controlled, but also the structure of the by-products of the oxidation reaction can be optimized to avoid secondary pollution.

[0116] Although ozone and oxidant treatment can significantly degrade viral nucleic acids and proteins, some incompletely degraded viral nucleic acid fragments or protein shells may still remain in the sewage. In order to completely inactivate the virus, further enzymes are needed for passivation treatment. After the reaction is completed, RNAse is added to the sewage at a concentration of 5-10 mg / L. RNAse can efficiently hydrolyze the phosphodiester bonds in the residual nucleic acids of the virus, causing it to completely lose its genetic function; in addition, by acting on the viral shell protein, its structural integrity is further weakened. When adding RNAse, it is necessary to ensure the uniform distribution of the enzyme in the sewage, which can be achieved by stirring or other mixing methods. The sewage system after addition must be kept uniform to ensure that the activity of the enzyme is fully exerted.

[0117] In order to ensure the catalytic activity of RNA enzymes and promote their efficient degradation of viral nucleic acids and protein shells, the temperature of sewage needs to be controlled in the range of 25-30°C. This temperature range can effectively avoid the inactivation of enzymes while ensuring that the degradation rate of viral components reaches the best. The passivation reaction time is generally controlled to be 10 minutes. During this time, RNA enzymes can fully play their role and achieve the expected degradation effect of viral residues. In order to improve the efficiency of the passivation reaction, the changes in nucleic acid concentration in sewage can be detected through an online monitoring system to ensure that the treatment effect meets the discharge standards.

[0118] This step effectively inhibits the formation of byproducts such as bromate and chlorate through the preferential reaction mechanism of competitive oxidants, and uses RNA enzymes to target and degrade residual viral nucleic acids and protein shells in sewage. Nitrates and nitrites, as competitive oxidants, can react preferentially with ozone, reducing the chance of ozone reacting with bromide and chloride ions; and precise control of pH further reduces the risk of chlorate formation. The efficient catalytic action of RNA enzymes ensures complete inactivation of the virus by cutting the phosphodiester bonds of viral nucleic acids and destroying the structure of the protein shell.

[0119] Please refer to the attached Figure 7 , S6: After the water outlet detection is performed, the drainage is completed.

[0120] S61: Detect the concentration of viral RNA or DNA in sewage by real-time fluorescence quantitative PCR;

[0121] S62: Testing the pH value, dissolved oxygen concentration and turbidity of the effluent;

[0122] S63: When the detection index does not meet the standard, the steps S2-S5 are cycled;

[0123] S64: When the test indicators meet the standards, the sewage will be discharged into the municipal drainage system or reused in non-drinking water treatment scenarios.

[0124] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A hospital sewage disinfection process with intelligent targeted virus disinfecting, characterized in that: The following steps are involved: S1: Prepare ozone micro-nano bubbles through a micro-nano bubble generator, and inject ozone gas into sewage to form micro-nano bubbles; S2: Adding oxidants to wastewater containing ozone micro-nano bubbles to destroy the lipid envelope of the virus; S3: Adjust the pH value of sewage and reaction time to degrade the protein shell of the virus through the synergistic effect of hydroxyl radicals and chemical modifiers; S4: Combining UV irradiation with a catalyst to destroy viral nucleic acids; S5: Control the generation of by-products during the reaction and inactivate residual viral components by adding enzymes; S6: After the water outlet detection is performed, the drainage is completed.

2. According to claim 1, a hospital sewage disinfection process for intelligent targeted virus disinfecting is characterized in that: The S1 step includes: S11: Generate ozone gas using an ozone generator, the concentration of which is controlled at 50-100 mg / L, and introduce it into a gas-liquid mixing system through a gas pipeline; S12: mixing the ozone gas with the sewage, and utilizing the turbulence effect to disperse the ozone gas into micro-nano bubbles with a diameter of 50-200 nm during the mixing process; S13: Distribute micro-nano bubbles in the sewage to control the concentration of ozone dissolved in the bubbles to 10-20ppm.

3. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 1 is characterized in that: The S2 step includes: S21: preparing an oxidant solution, wherein the oxidant is a natural polyphenol compound; S22: slowly adding the prepared oxidant solution into the sewage in batches according to the viral load; S23: Oxidation of fatty acid molecules in the viral lipid envelope through the interfacial effect of ozone micro-nanobubbles; S24: The oxidation reaction is maintained under the condition that the sewage temperature is controlled at 25-30°C for a duration of 10-15 minutes.

4. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 1 is characterized in that: The S3 step includes: S31: adjusting the pH value of the sewage to 6.5-7.5 by adding an acid or alkaline agent; S32: maintaining the reaction temperature at 25-30°C and controlling the reaction time to 10-20 min; S33: introducing a chemical modifier into the sewage, the chemical modifier including but not limited to guanidinoacetic acid or urea, with a concentration of 5-10 mg / L; S34: Ozonolysis and oxidant action synergistically generate hydroxyl radicals, which react with key amino acids in the viral protein coat.

5. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 1 is characterized in that: The S4 step includes: S41: Adjust the pH value of sewage to maintain at 6.5-7.5; S42: UV irradiation of wastewater to directly destroy the phosphodiester bonds of viral nucleic acids; S43: adding a catalyst to the sewage while irradiating with ultraviolet light; S44: Generates hydroxyl radicals, superoxide ions and elemental oxygen through catalytic reactions.

6. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 1 is characterized in that: The S5 step includes: S51: adding one or more of nitrate and nitrite to the sewage as competitive oxidants, with the dosage controlled at 1-3 mg / L; S52: Adjust the pH value of sewage to 6.5-7.5 to inhibit chlorate formation; S53: After the reaction is completed, RNase is added to inactivate the residual viral nucleic acid and viral protein shell in the sewage. The enzyme addition concentration is controlled at 5-10 mg / L; S54: Maintain the temperature of the enzyme inactivation reaction at 25-30°C, and the reaction time is 10 minutes.

7. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 1 is characterized in that: The S6 step includes: S61: Detect the concentration of viral RNA or DNA in sewage by real-time fluorescence quantitative PCR; S62: Testing the pH value, dissolved oxygen concentration and turbidity of the effluent; S63: When the detection index does not meet the standard, the steps S2-S5 are cycled; S64: When the test indicators meet the standards, the sewage will be discharged into the municipal drainage system or reused in non-drinking water treatment scenarios.

8. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 2 is characterized in that: The concentration of the oxidant solution in S21 is 10-30 mg / L, and the oxidant solution includes but is not limited to catechol or gallic acid.

9. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 5 is characterized in that: In the step S42, the wavelength of ultraviolet light is set to 180-254 nm, and the irradiation intensity is controlled to 20-40 mJ / cm 2 The ultraviolet action time is 5-10min.

10. The hospital sewage disinfection process for intelligent targeted virus disinfecting according to claim 5 is characterized in that: The catalyst in step S43 is, but is not limited to, Fe 2+ , Mn 2+ Or TiO2, ZnO, the concentration of the catalyst is controlled at 1-3 mg / L.

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