Oral care solution and preparation method thereof

Abstract

The invention discloses an oral care solution and a preparation method thereof. Comprising the following raw materials in parts by weight: 10-20 parts of a traditional Chinese medicine extract, 5-10 parts of organic acid, 1-3 parts of essence, 3-5 parts of a preservative, 1-3 parts of a sweetening agent, 3-5 parts of glycerol and 50-100 parts of water. The oral care solution disclosed by the invention contains the traditional Chinese medicine extract components, has a good sterilizing effect and also has an effect of treating oral ulcer. In the components, the herba eupatorii extract and the junci medulla extract are combined for use, the lophatherum gracile extract and the crithmum maritimum extract are combined for use, the antibacterial performance is improved, the chlorogenic acid and the phenylalanine are combined for use, the effect of treating oral ulcer is improved, and the chlorogenic acid and the phenylalanine have a synergistic effect.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care, and in particular relates to an oral care liquid and a preparation method thereof. Background Art

[0002] Oral care liquid is a liquid product used to clean and care for the oral cavity, usually containing antibacterial ingredients, which can help reduce oral bacteria, prevent oral diseases, and freshen breath. According to the QB / T 2945-2012 standard, oral care liquid includes mouthwash, oral spray, etc., which are suitable for intraoral application. The main ingredients of these products include flavors, surfactants, fluoride, chloride, alcohol and water, which have a refreshing effect when entering the mouth.

[0003] In clinical applications, oral care solutions are widely used in the prevention and treatment of various oral diseases. For example, Chinese medicine oral care solutions can improve gum health, prevent gum bleeding, and have a significant hemostatic effect on patients taking oral warfarin. In addition, Western medicine preparations such as saline, hydrogen peroxide, sodium bicarbonate, and chlorhexidine are also used in clinical oral care operations to achieve the purpose of cleaning and antibacterial.

[0004] The market prospects for oral care solutions are broad, and they have developed rapidly in recent years, with new formulas and new dosage forms emerging. Global market analysis shows that the demand and production capacity of oral care solutions are growing steadily, especially in North America, Europe and China. This growth trend reflects people's increasing attention to oral health and the increasing demand for convenient and effective oral care products. Oral care solutions are not only widely used in daily life, but also play an important role in the medical field. Its diverse ingredients and functions make it an important tool for maintaining oral health. The problem with current oral care solutions is that the antibacterial and antimicrobial efficacy is relatively low, especially the inhibitory effect on oral pathogens Streptococcus mutans and Porphyromonas gingivalis is poor, and the treatment effect on oral ulcers is poor. Summary of the invention

[0005] The object of the present invention is to provide an oral care liquid and a preparation method thereof.

[0006] An oral care liquid comprises the following raw materials in parts by weight: 10-20 parts of a traditional Chinese medicine extract, 5-10 parts of an organic acid, 1-3 parts of an essence, 3-5 parts of a preservative, 1-3 parts of a sweetener, 3-5 parts of glycerol, and 50-100 parts of water.

[0007] The Chinese medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:1.

[0008] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 7-15 times the weight of water and soaking for 30-60 minutes, then reflux extraction for 1-3 times, each time for 30-60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0009] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 8-12 times the weight of water and soaking for 30-60 minutes, then reflux extraction for 1-3 times, each time for 30-60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0010] The Chinese medicine extract is a mixture of a bamboo leaf extract and a sea fennel extract in a mass ratio of 3:1.

[0011] The preparation method of the Bambusa damicornis leaf extract comprises the following steps: taking Bambusa damicornis leaf, adding 10-15 times the weight of water and soaking for 30-50 minutes, then reflux extracting for 1-3 times, each time for 30-50 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0012] The preparation method of the sea fennel extract comprises the following steps: taking sea fennel, adding 1-3 times the weight of water to pulp, then adding 5-10 times the weight of water, and then reflux extraction for 1-3 times, each time for 30-50 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0013] The organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0014] The flavor is one or more of mint flavor, violet flavor, rose flavor, jasmine flavor, tuberose flavor, and milk flavor.

[0015] The preservative is one or more of methyl parahydroxybenzoate, propyl parahydroxybenzoate, sodium benzoate and sorbic acid.

[0016] The sweetener is one or more of sucrose, glucose, fructose and xylitol.

[0017] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: adding organic acid, flavor, preservative, sweetener and glycerin into water, heating to 50-60°C, stirring at 500-1000rpm for 10-30min, cooling to room temperature, adding Chinese medicine extract and continuing stirring for 10-20min.

[0018] Beneficial effects of the present invention: The oral care solution of the present invention contains Chinese medicine extract components, has good bactericidal effect, and also has the effect of treating oral ulcers. The components include the combination of Perilla frutescens extract and Juncus effusus extract, the combination of Bamboo leaf extract and Crimson foenum-leaf extract, which improves the antibacterial performance, and the combination of chlorogenic acid and phenylalanine, which improves the effect of treating oral ulcers, and all of them are synergistic. DETAILED DESCRIPTION

[0019] In order to facilitate the understanding of the present invention, the present invention will be described more fully below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0020] Example 1

[0021] An oral care liquid comprises the following raw materials in parts by weight: 15 parts of Chinese herbal medicine extract, 8 parts of organic acid, 2 parts of mint essence, 4 parts of methyl parahydroxybenzoate, 2 parts of sucrose, 4 parts of glycerol, and 80 parts of water; the Chinese herbal medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:1; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0022] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 12 times the weight of water and soaking for 40 minutes, then reflux extraction for 2 times, each time for 40 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

[0023] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 10 times the weight of water and soaking for 40 minutes, then reflux extraction for 2 times, each time for 40 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

[0024] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, mint essence, methyl paraben, sucrose and glycerin are added to water, the temperature is raised to 55°C, stirred at 800rpm for 20min, cooled to room temperature (25°C), and the Chinese medicine extract is added and stirred for 15min.

[0025] Example 2

[0026] An oral care liquid comprises the following raw materials in parts by weight: 12 parts of Chinese herbal medicine extract, 6 parts of organic acid, 1 part of rose essence, 3 parts of sodium benzoate, 1 part of xylitol, 3 parts of glycerol, and 60 parts of water; the Chinese herbal medicine extract is a mixture of a bamboo leaf extract and a sea fennel extract in a mass ratio of 3:1; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0027] The preparation method of the bamboo leaf extract comprises the following steps: taking bamboo leaves, adding 10 times the weight of water and soaking for 45 minutes, then reflux extraction for 3 times, each time for 30 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

[0028] The preparation method of the sea fennel extract comprises the following steps: taking sea fennel, adding 1 times the weight of water to pulp, then adding 8 times the weight of water, and then reflux extraction for 3 times, each time for 30 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0029] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, flavor, preservative, sweetener, and glycerin are added into water, the temperature is raised to 50°C, stirred at 500rpm for 30min, cooled to room temperature (25°C), and the Chinese medicine extract is added and stirred for 12min.

[0030] Example 3

[0031] An oral care liquid comprises the following raw materials in parts by weight: 20 parts of Chinese herbal medicine extract, 10 parts of organic acid, 3 parts of jasmine essence, 5 parts of propyl paraben, 3 parts of fructose, 5 parts of glycerol, and 100 parts of water; the Chinese herbal medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:1; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0032] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 13 times the weight of water, soaking for 50 minutes, then reflux extraction once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0033] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 12 times the weight of water and soaking for 60 minutes, then refluxing and extracting once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0034] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, jasmine essence, propyl paraben, fructose and glycerin are added to water, the temperature is raised to 60°C, stirred at 1000rpm for 10 minutes, cooled to room temperature (25°C), and the Chinese medicine extract is added and stirred for 20 minutes.

[0035] Comparative Example 1

[0036] An oral care liquid comprises the following raw materials in parts by weight: 15 parts of perilla extract, 8 parts of organic acid, 2 parts of mint essence, 4 parts of methyl parahydroxybenzoate, 2 parts of sucrose, 4 parts of glycerol, and 80 parts of water; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0037] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 12 times the weight of water and soaking for 40 minutes, then reflux extraction for 2 times, each time for 40 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

[0038] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: add organic acid, mint essence, methyl paraben, sucrose and glycerin into water, heat it to 55°C, stir it at 800rpm for 20min, cool it to room temperature (25°C), add perilla extract and continue stirring for 15min.

[0039] Comparative Example 2

[0040] An oral care liquid comprises the following raw materials in parts by weight: 15 parts of rush extract, 8 parts of organic acid, 2 parts of mint essence, 4 parts of methyl parahydroxybenzoate, 2 parts of sucrose, 4 parts of glycerol, and 80 parts of water; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0041] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 10 times the weight of water and soaking for 40 minutes, then reflux extracting for 2 times, each time for 40 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0042] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, mint essence, methyl paraben, sucrose and glycerin are added to water, the temperature is raised to 55°C, stirred at 800rpm for 20min, cooled to room temperature (25°C), and rush extract is added and stirred for 15min.

[0043] Comparative Example 3

[0044] An oral care liquid comprises the following raw materials in parts by weight: 12 parts of loblolly leaf extract, 6 parts of organic acid, 1 part of rose essence, 3 parts of sodium benzoate, 1 part of xylitol, 3 parts of glycerol, and 60 parts of water; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0045] The preparation method of the Bambusa chinensis leaf extract comprises the following steps: taking Bambusa chinensis leaves, adding 10 times the weight of water and soaking for 45 minutes, then reflux extracting for 3 times, each time for 30 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

[0046] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, flavor, preservative, sweetener, and glycerin are added into water, the temperature is raised to 50°C, stirred at 500rpm for 30min, cooled to room temperature (25°C), and light bamboo leaf extract is added and stirred for 12min.

[0047] Comparative Example 4

[0048] An oral care liquid comprises the following raw materials in parts by weight: 12 parts of sea fennel extract, 6 parts of organic acid, 1 part of rose essence, 3 parts of sodium benzoate, 1 part of xylitol, 3 parts of glycerol, and 60 parts of water; the organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:1.

[0049] The preparation method of the sea fennel extract comprises the following steps: taking sea fennel, adding 1 times the weight of water to pulp, then adding 8 times the weight of water, and then reflux extraction for 3 times, each time for 30 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0050] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: organic acid, flavor, preservative, sweetener and glycerin are added into water, the temperature is raised to 50°C, stirred at 500 rpm for 30 minutes, cooled to room temperature (25°C), and sea fennel extract is added and stirred for 12 minutes.

[0051] Comparative Example 5

[0052] An oral care liquid comprises the following raw materials in parts by weight: 20 parts of Chinese herbal medicine extract, 10 parts of chlorogenic acid, 3 parts of jasmine essence, 5 parts of propylparaben, 3 parts of fructose, 5 parts of glycerol, and 100 parts of water; the Chinese herbal medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:1.

[0053] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 13 times the weight of water, soaking for 50 minutes, then reflux extraction once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0054] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 12 times the weight of water and soaking for 60 minutes, then refluxing and extracting once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0055] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: chlorogenic acid, jasmine essence, propyl paraben, fructose and glycerol are added to water, the temperature is raised to 60°C, stirred at 1000rpm for 10min, cooled to room temperature (25°C), and the Chinese medicine extract is added and stirred for 20min.

[0056] Comparative Example 6

[0057] An oral care liquid comprises the following raw materials in parts by weight: 20 parts of Chinese herbal medicine extract, 10 parts of phenylalanine, 3 parts of jasmine essence, 5 parts of propylparaben, 3 parts of fructose, 5 parts of glycerol, and 100 parts of water; the Chinese herbal medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:1.

[0058] The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 13 times the weight of water, soaking for 50 minutes, then reflux extraction once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0059] The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 12 times the weight of water and soaking for 60 minutes, then refluxing and extracting once, each time for 60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

[0060] The preparation method of the above-mentioned oral care liquid is carried out according to the following steps: phenylalanine, jasmine essence, propyl paraben, fructose and glycerol are added to water, the temperature is raised to 60°C, stirred at 1000rpm for 10min, cooled to room temperature (25°C), and the Chinese medicine extract is added and stirred for 20min.

[0061] Experimental example:

[0062] Bacterial inhibition zone determination: 200 μL of the prepared Streptococcus mutans and Porphyromonas gingivalis bacterial liquid were respectively drawn onto the surface of the BHI culture medium, and evenly coated with a sterile cotton swab, and placed at room temperature for 4 minutes. After the bacterial liquid on the surface of the plate was absorbed, a sterile Oxford cup with a diameter of 6 mm was fixed on the culture medium, and the oral care liquid prepared in each embodiment and comparative example was marked, and 3 Oxford cups were placed in each culture medium. Then, 50 μL of each oral care liquid was added to each Oxford cup with a pipette, and each strain was repeated 3 times. After culturing for 4 days under anaerobic conditions at 37°C, the presence and diameter of the antibacterial zone were observed and recorded.

[0063] The experimental results were statistically analyzed using SPSS 24.0 software, and the quantitative data were analyzed using (mean ± standard deviation), the Kolmogorov-Smirnov test was used for data normality test, and for data that met the normal distribution, the t-test was used to compare the mean differences between the two groups, with P < 0.05 as the difference being statistically significant. The test results are shown in Table 1-2:

[0064] Table 1

[0065]

[0066] Note: * represents P < 0.05 compared with the group in Example 1.

[0067] Table 2

[0068]

[0069] Note: * represents P < 0.05 compared with the group in Example 2.

[0070] Oral ulcer experiment: After the rats were anesthetized with ether, a piece of rat oral mucosal skin was broken with tweezers, and Staphylococcus aureus liquid was injected subcutaneously into the broken mucosa. The ulcer surface surrounded by yellow exudate was formed every other day. The rats with successful modeling were taken, half male and half female, weighing about 200g, and randomly divided into 5 groups after weighing, with 10 rats in each group. They were divided into a blank group, a positive control group and a drug-treated group (oral care solution prepared in Example 3 and Comparative Examples 5-6). Each group was given medicine twice a day. The blank group was directly given a medical gauze piece without medicine to apply on the ulcer surface; the positive control group was given a medical gauze piece dipped in vitamin B12 for application; the drug-treated group was given a medical gauze piece dipped in oral care solution for application. Before the first day of administration, the diameter of the ulcer surface of each rat was measured with a vernier caliper. The drug was administered continuously and observed for 5 days. The average healing diameter was used as an indicator to evaluate the efficacy. The results are shown in Table 3:

[0071] Table 3

[0072]

[0073] Note: * represents P < 0.05 compared with the group in Example 3.

[0074] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An oral care solution, characterized in that: The invention comprises the following raw materials in parts by weight: 10-20 parts of Chinese herbal medicine extract, 5-10 parts of organic acid, 1-3 parts of flavor, 3-5 parts of preservative, 1-3 parts of sweetener, 3-5 parts of glycerol and 50-100 parts of water.

2. The oral care solution according to claim 1, characterized in that: The Chinese medicine extract is a mixture of perilla extract and rush extract in a mass ratio of 1:

1.

3. The oral care solution according to claim 2, characterized in that: The preparation method of the perilla extract comprises the following steps: taking perilla stems and leaves, adding 7-15 times the weight of water and soaking for 30-60 minutes, then reflux extraction for 1-3 times, each time for 30-60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

4. The oral care solution according to claim 2, characterized in that: The preparation method of the rush extract comprises the following steps: taking the whole rush, adding 8-12 times the weight of water and soaking for 30-60 minutes, then reflux extraction for 1-3 times, each time for 30-60 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

5. The oral care solution according to claim 1, characterized in that: The Chinese medicine extract is a mixture of a bamboo leaf extract and a sea fennel extract in a mass ratio of 3:

1.

6. The oral care solution according to claim 5, characterized in that: The preparation method of the Bambusa dahliae extract comprises the following steps: taking Bambusa dahliae, adding 10-15 times the weight of water and soaking for 30-50 minutes, then reflux extracting for 1-3 times, each time for 30-50 minutes, combining the filtrates, filtering with activated carbon, and evaporating to dryness.

7. The oral care solution according to claim 5, characterized in that: The preparation method of the sea fennel extract comprises the following steps: taking sea fennel, adding 1-3 times the weight of water to pulp, then adding 5-10 times the weight of water, and then reflux extraction for 1-3 times, each time for 30-50 minutes, combining the filtrate, filtering with activated carbon, and evaporating to dryness.

8. The oral care solution according to claim 1, characterized in that: The organic acid is a mixture of chlorogenic acid and phenylalanine in a mass ratio of 2:

1.

9. The oral care solution according to claim 1, characterized in that: The flavor is one or more of mint flavor, violet flavor, rose flavor, jasmine flavor, tuberose flavor, and milk flavor; the preservative is one or more of methyl parahydroxybenzoate, propyl parahydroxybenzoate, sodium benzoate, and sorbic acid; the sweetener is one or more of sucrose, glucose, fructose, and xylitol.

10. The method for preparing the oral care solution according to claim 1, characterized in that: The process is as follows: add organic acid, flavor, preservative, sweetener and glycerin into water, heat to 50-60°C, stir at 500-1000rpm for 10-30min, cool to room temperature, add Chinese herbal extract and continue stirring for 10-20min.

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