A strain of Bacillus coagulans that facilitates cell recovery and its application in lactic acid fermentation
By screening out the Bacillus coagulans strain LL08, which has natural sedimentation characteristics, and combining it with a flocculant, the problem of high cost of cell reuse after Bacillus coagulans fermentation was solved, achieving efficient cell recovery and high optical purity lactic acid production.
CN119662475BActive Publication Date: 2026-05-26JIANGNAN UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2024-12-20
- Publication Date
- 2026-05-26
AI Technical Summary
Technical Problem
The current method of reusing Bacillus coagulans fermentation cells requires centrifugation, which leads to high production costs and affects the production of high optical purity lactic acid.
Method used
A Bacillus coagulans strain, LL08, was screened out. It has natural sedimentation characteristics and, when combined with a flocculant, enables the natural sedimentation and recovery of the bacteria, reducing the amount of organic nitrogen source used.
Benefits of technology
Through natural sedimentation and flocculation recovery, production costs are significantly reduced, and the optical purity and production efficiency of lactic acid fermentation are improved.
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Figure CN119662475B_ABST
Abstract
This invention discloses a strain of *Bacillus coagulans* that facilitates cell recovery and its application in lactic acid fermentation, belonging to the fields of microbiology and fermentation engineering technology. This invention isolates a batch of thermoresistant bacteria from spoiled milk. After small-scale cell culture, a flocculant is added to observe sedimentation. The strain with the fastest sedimentation rate is selected, and *Bacillus coagulans* is identified among them. Further screening yields a strain, XQ20249, with rapid cell sedimentation and high optical purity of L-lactic acid produced during fermentation. Further mutagenesis using nitrosoguanidine and screening yields mutant strains with even faster cell sedimentation. Among them, mutant strain LL08 shows a significant increase in flocculation and exhibits faster growth and lactic acid fermentation rates than other mutant strains under lactic acid fermentation conditions. When strain LL08 is used for lactic acid fermentation, the cells can be collected by sedimentation after fermentation for secondary fermentation, significantly reducing the amount of organic nitrogen source used.
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