Seed preservation method of mangrove plant bruguiera gymnorrhiza

Abstract

The invention discloses a seed preservation method of mangrove plant bruguiera gymnorrhiza. The method comprises three steps of seed pretreatment, seed preservation and seed germination. The method specifically comprises the following steps: cleaning and disinfecting bruguiear gymnorrhiza seeds, wiping water, adjusting the water content of the bruguiear gymnorrhiza seeds to 90%, smearing vaseline on the surfaces of the seeds, putting the seeds in sand with the humidity of about 60%, putting the bruguiear gymnorrhiza seeds and the sand into a double-layer plastic package bag together, sealing, and preserving in a dark place at 17 DEG C. The germination rate of the bruguiear gymnorrhiza seeds preserved by the method can still reach 100% after 6 months, and timely and rapid reseeding of the bruguiear gymnorrhiza seeds can be realized.

Description

Technical Field

[0001] The invention belongs to the technical field of plant seed preservation, and in particular relates to a method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza. Background Art

[0002] Bruguiera gymnorrhiza Bruguiera gymnorrhiza ) is a viviparous mangrove plant of the genus Bruguiera in the family Rhizophoraceae. It is widely distributed in my country and is one of the dominant species of mangroves. Mangroves can not only serve as "coastal guards" to break wind and waves and purify seawater, but also provide habitats for many organisms and are "sea forests" with carbon fixation capabilities. However, over the past half century, coastal development, drainage, reclamation, aquaculture, and environmental pollution in mangrove areas have continued to emerge. Due to the strong interference of human activities, the area of ​​mangroves has sharply decreased worldwide. Therefore, it is urgent to carry out mangrove afforestation.

[0003] Bruguiera gymnorrhiza seeds are spindle-shaped, dark green, 14-20cm long, 1.4-1.8cm wide, and each fresh weight is 15-30g. After harvesting, Bruguiera gymnorrhiza seeds are prone to insect rot and natural water loss, which makes them lose vitality. Afforestation with Bruguiera gymnorrhiza seeds is often affected by weather and tides and cannot be planted in time. After planting, the survival rate is very low due to poor habitat conditions, and replanting is often required. Since Bruguiera gymnorrhiza seeds are not easy to preserve, the afforestation work of Bruguiera gymnorrhiza is restricted. Therefore, finding a long-term preservation method for Bruguiera gymnorrhiza seeds is the key to the ecological restoration of Bruguiera gymnorrhiza mangroves.

[0004] There is no relevant preservation method for Bruguiera gymnorrhiza seeds at present. This patent can prolong the survival time of Bruguiera gymnorrhiza seeds by controlling the moisture content of seeds, applying preservatives, sealing them with sand, and finally storing the seeds at low temperature and away from light, so as to achieve timely and rapid replanting of Bruguiera gymnorrhiza seeds. Summary of the invention

[0005] The object of the present invention is to provide a method for preserving seeds of the mangrove plant Bruguiera gymnorrhiza, which can get rid of the dependence of Bruguiera gymnorrhiza seed germination on the seed maturation period by controlling the seed water content and the water loss during the preservation process, and realize the planting of Bruguiera gymnorrhiza at different time periods.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: A method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza comprises the following steps: (1) Seed pretreatment: After cleaning the Bruguiera gymnorrhiza seeds, disinfect them with carbendazim solution for 15 min, wipe the surface moisture of the Bruguiera gymnorrhiza seeds dry, and select the Bruguiera gymnorrhiza seeds with uniform shape, no obvious diseases and insect pests, thick and long, and dark green color; (2) Seed preservation: The moisture content of the Bruguiera gymnorrhiza seeds obtained in step (1) is adjusted to 90%, a layer of vaseline is applied on the surface, and the seeds are placed in sand, sealed in a double-layer plastic bag, and stored away from light; (3) Seed germination: Take out the Bruguiera gymnorrhiza seeds prepared in step (2) and plant them in a seedling bag filled with a sand-mud mixture. Insert the root of the Bruguiera gymnorrhiza seed downward into the seedling bag to a depth of half the length of the Bruguiera gymnorrhiza seed. Leave a 2 cm space between the bottom of the seed and the bottom of the seed. After watering, move the seedling bag to the germination area and wait for the seeds to germinate.

[0007] The carbendazim solution in step (1) is a carbendazim powder containing 50% of the active ingredient, which is dissolved in water at a ratio of 1 g: 500 mL, and the water temperature is 25°C.

[0008] The method for adjusting the moisture content in step (2) is to place the Bruguiera gymnorrhiza seeds in an oven for 12 hours at a temperature of 40°C.

[0009] The humidity of the sand in step (2) is 60%.

[0010] The light-proof storage temperature in step (2) is 17°C.

[0011] The content of sand in the sand-mud mixture in step (3) is 50%.

[0012] The light intensity in the germination zone in step (3) is 120 μmol·m -2 ·s -1 , the light duration is 12 hours per day; the ambient temperature is 26℃, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist. The substrate used is a sand-mud mixture, in which the sand content is 50%.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The present invention gets rid of the dependence of Bruguiera gymnorrhiza seed germination on the seed maturation period by setting different temperatures, preservatives and water contents. The germination rate of Bruguiera gymnorrhiza seeds preserved by the method can still reach 100% after 6 months, and the planting of Bruguiera gymnorrhiza at different time periods can be realized. DETAILED DESCRIPTION

[0014] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] The carbendazim powder in the following embodiment is Kunning Wangpai carbendazim produced by Liaoning Yingkou Leike Pesticide Co., Ltd.; the mud comes from the beach in Jinjiang City, Quanzhou City, Fujian Province. The seed pretreatment step is to wash the Bruguiera gymnorrhiza seeds and disinfect them with a carbendazim solution for 15 minutes. The carbendazim solution is a carbendazim powder with an effective ingredient of 50% dissolved in water at a ratio of 1g: 500mL, and the water temperature is 25°C. After that, the surface moisture of the Bruguiera gymnorrhiza seeds is wiped dry, and the Bruguiera gymnorrhiza seeds with uniform morphology, no obvious pests and diseases, thick and long, and dark green color are screened out.

[0016] Example 1 Effect of different water contents on the preservation of Bruguiera gymnorrhiza seeds The moisture content of Bruguiera gymnorrhiza seeds was set to 100% (fresh seeds), 90% and 80% respectively. The specific steps were as follows: first weigh the weight of fresh Bruguiera gymnorrhiza seeds, then place the weighed seeds in a 40°C oven for 12 hours, take out the seeds regularly and weigh them during this period. When the seeds lose 10% of their weight, their moisture content is 90%. Similarly, when the seeds are placed in a 40°C oven for 24 hours and lose 20% of their weight, their moisture content is 80%.

[0017] Other conditions are as follows: the storage method is sand burial, the sand humidity is 60%, the sand and the above-mentioned Bruguiera gymnorrhiza seeds are placed in a double-layer plastic bag and sealed, the storage temperature is 9°C, and it is protected from light.

[0018] The preserved seeds were taken out every other month for germination experiment. The specific steps of the germination experiment were as follows: the preserved Bruguiera gymnorrhiza seeds were planted in a seedling bag filled with a sand-mud mixture (sand content 50%). The Bruguiera gymnorrhiza seeds were inserted into the seedling bag with the roots down, with a depth of half the seeds. At the same time, there was a space of about 2 cm between the bottom of the seedling bag and the bottom of the seed. After watering, the seedling bag was moved to the germination area and the seeds were allowed to germinate. The light intensity during germination was 120 μmol·m -2 ·s -1 The light duration is 12 h / d, and the lamp is the ZK-TB18-VE02 / A lamp produced by Zhongke Sanan. The ambient temperature is 26℃, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0019] Table 1 Effects of different water contents on the germination rate of the mangrove plant Bruguiera gymnorrhiza As shown in Table 1, when the moisture content is 90%, the storage time of Bruguiera gymnorrhiza increases. When the moisture content is 90%, the germination rate is still 20% after being stored for one month.

[0020] Example 2 Effects of different preservatives on the preservation of Bruguiera gymnorrhiza seeds The preservatives used for coating were shellac and vaseline, and the water content of the seeds was 100%. Other conditions were as follows: the storage method was sand burial, the humidity of the sand was 60%, the water content of the seeds was 100%, and the sand and the above-mentioned Bruguiera gymnorrhiza seeds were placed in a double-layer plastic bag and sealed, and the storage temperature was 9°C and protected from light. The stored seeds were taken out every other month for germination experiments, and the experimental steps were the same as in Example 1. The light intensity during germination was 120 μmol·m -2 ·s -1 The light duration is 12h / d, and the lamp is the ZK-TB18-VE02 / A lamp produced by Zhongke Sanan. The ambient temperature is 26℃, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0021] Table 2 Effects of applying different preservatives on the germination rate of the mangrove plant Bruguiera gymnorrhiza As shown in Table 2, when the preservatives applied were vaseline and shellac, the storage time of Bruguiera gymnorrhiza increased significantly. The germination rate of vaseline was still 20% after 3 months of storage, and the germination rate of shellac was 40% after 2 months of storage.

[0022] Example 3 Effect of different temperatures on the preservation of Bruguiera gymnorrhiza seeds The storage temperature was set to 4°C, 9°C, 14°C, and 17°C. Other conditions were as follows: the seeds were buried in sand with a humidity of 60%. The sand and the seeds were sealed in a double-layer plastic bag and kept away from light. The seeds were taken out every other month for germination experiments. The experimental steps were the same as in Example 1. The light intensity during germination was 120 μmol·m -2 ·s -1 The light duration is 12h / d, and the lamp is the ZK-TB18-VE02 / A lamp produced by Zhongke Sanan. The ambient temperature is 26℃, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0023] Table 3 Effects of storage temperature on germination rate of mangrove plant Bruguiera gymnorrhiza As shown in Table 3, when the storage temperature is 17℃, the storage time of Bruguiera gymnorrhiza increases significantly. When the storage time reaches 4 months, the germination rate is still 80%, but when the storage time reaches 5 months and 6 months, the germination rate is 0%.

[0024] Example 4 Effect of Optimal Conditions on the Preservation of Bruguiera gymnorrhiza Seeds After setting the moisture content of Bruguiera gymnorrhiza seeds to 90%, vaseline was applied to the surface of the seeds and stored in a dark environment at 17°C. Other conditions: storage was carried out by burying in sand, the humidity of the sand was 60%, and the sand and the above Bruguiera gymnorrhiza seeds were placed in a double-layer plastic bag and sealed, and stored in a dark environment. The stored seeds were taken out every other month for germination experiments, and the experimental steps were the same as in Example 1. The light intensity during germination was 120 μmol·m -2 ·s -1 The light duration is 12h / d, and the lamp is ZK-TB18-VE02 / A produced by Zhongke Sanan. The ambient temperature is 26℃, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0025] Table 4 Effects of optimal conditions on germination rate of mangrove plant Bruguiera gymnorrhiza As shown in Table 4, when the optimal conditions were adopted, the storage time of Bruguiera gymnorrhiza increased significantly, and the germination rate was still 100% when the storage time reached 6 months.

[0026] In summary, when the moisture content of Bruguiera gymnorrhiza seeds is controlled at 90%, 20% of the seeds can still germinate after being stored for 1 month (Table 1). Bruguiera gymnorrhiza seeds still have a 20% germination rate after being coated with vaseline and stored for 3 months (Table 2). Bruguiera gymnorrhiza seeds still have a 100% germination rate after being stored at 17°C for 6 months (Table 3). When the optimal conditions are adopted, the germination rate is still 100% after being stored for 6 months (Table 4). It can be seen that after the moisture content of Bruguiera gymnorrhiza seeds is adjusted to 90%, vaseline is applied, and they are placed in sand with a humidity of about 60%. The Bruguiera gymnorrhiza seeds and sand are placed in a double-layer plastic bag and sealed, and kept at 17°C away from light, which is a suitable storage condition for Bruguiera gymnorrhiza seeds.

[0027] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A method for preserving seeds of a mangrove plant, Bruguiera gymnorrhiza, characterized in that: The following steps are involved: (1) Seed pretreatment: After cleaning the Bruguiera gymnorrhiza seeds, disinfect them with carbendazim solution for 15 min, wipe the surface moisture of the Bruguiera gymnorrhiza seeds dry, and select the Bruguiera gymnorrhiza seeds with uniform shape, no obvious diseases and insect pests, thick and long, and dark green color; (2) Seed preservation: The moisture content of the Bruguiera gymnorrhiza seeds obtained in step (1) is adjusted to 90%, a layer of vaseline is applied on the surface, and the seeds are placed in sand, sealed in a double-layer plastic bag, and stored away from light; Seed germination: Take out the Bruguiera gymnorrhiza seeds in step (2) and plant them in a seedling bag filled with a sand-mud mixture. Insert the root of the Bruguiera gymnorrhiza seed downward into the seedling bag to a depth of half the length of the Bruguiera gymnorrhiza seed. Leave a 2 cm space between the bottom of the seed and the bottom of the seed. After watering, move the seedling bag to the germination area and wait for the seeds to germinate.

2. The method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The carbendazim solution in step (1) is a carbendazim powder containing 50% of the active ingredient, which is dissolved in water at a ratio of 1 g: 500 mL, and the water temperature is 25°C.

3. The method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The method for adjusting the moisture content in step (2) is to place the Bruguiera gymnorrhiza seeds in an oven for 12 hours at a temperature of 40°C.

4. The method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The humidity of the sand in step (2) is 60%.

5. The method for preserving seeds of a mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The light-proof storage temperature in step (2) is 17°C.

6. The method for preserving seeds of the mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The content of sand in the sand-mud mixture in step (3) is 50%.

7. The method for preserving seeds of the mangrove plant Bruguiera gymnorrhiza according to claim 1, characterized in that: The light intensity in the germination zone in step (3) is 120 μmol·m -2 ·s -1 The daily lighting duration is 12 hours; the ambient temperature is 26°C, the relative humidity is 80%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist; the substrate used is a sand-mud mixture, in which the sand content is 50%.

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