Transparent protein electrolyte sports beverage and preparation method thereof

Abstract

The invention relates to the technical field of food, in particular to a transparent protein electrolyte sports beverage which comprises the following raw materials: nanofiltration filtrate, sugar, decolored and deacidified apple juice concentrate, whey protein, an acidity regulator, Acai berry extract, acerola cherry concentrated powder, natural lemon essence and water. The preparation method comprises the following steps: mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain feed liquid A; mixing the decolored and deacidified apple concentrated juice, an acidity regulator, an Acai berry extract, acerola cherry concentrated powder and water to obtain feed liquid B; cooling the feed liquid A, and mixing the cooled feed liquid A with the feed liquid B to obtain feed liquid C; and sterilizing, degassing, cooling and filling the feed liquid C to obtain the transparent protein electrolyte sports beverage. The prepared beverage is high in electrolyte content, attractive in color, fresh and cool in taste and free of acerbity, and the beverage is bright and transparent pink within the normal-temperature shelf life of 6 months.

Description

Technical Field

[0001] The invention relates to the technical field of food, in particular to a transparent protein electrolyte sports drink and a preparation method thereof. Background Art

[0002] In early 2023, sports drinks with electrolytes as a key ingredient saw a surge in demand due to their ability to help relieve dehydration caused by symptoms such as fever. The focus on sports scenes and an active lifestyle have jointly contributed to the growth of the sports drink market.

[0003] Physical activity is a catalyst for the human body's metabolic process. Once the human body enters a state of exercise, the body's energy metabolism and material metabolism will be enhanced. In order to maintain the body's energy supply and heat balance during exercise, a large amount of energy substances are consumed, and various electrolytes are lost as sweat dissipates heat. The consumption of energy substances and the loss of electrolytes are important reasons for the decline in human athletic performance. Studies have shown that supplementing various nutrients required for exercise during exercise is beneficial to delaying the occurrence of fatigue and accelerating fatigue recovery.

[0004] Sports drinks are formulated according to the characteristics of physiological consumption during exercise. They can specifically replenish some of the nutrients lost during exercise, maintain and improve athletic ability, and accelerate the elimination of fatigue after exercise. The substances usually added are carbohydrates, vitamins, proteins, electrolytes, etc. The human body needs sports drinks to quickly replenish water, energy and electrolytes, and help relieve and eliminate muscle fatigue after exercise. Therefore, sports drinks contain an appropriate amount of monosaccharides or disaccharides to provide energy; they need to contain an appropriate amount of electrolytes such as sodium, potassium and calcium to replenish the electrolyte loss caused by sweating during exercise; in addition, they need to supplement an appropriate amount of protein to repair muscle damage, relieve muscle fatigue, and improve athletic ability.

[0005] Sports drinks on the market are basically electrolyte drinks that simply add sugars, vitamins, and minerals. These drinks currently have the following problems: (i) they are not able to quench thirst and eliminate fatigue quickly enough; (ii) the electrolytes they supplement are not easily absorbed by the human body due to the mutual interference and repulsion between certain elements; and (iii) sports drinks have poor palatability, unpleasant flavors, and poor taste, and cannot meet the requirements of various sports individuals in improving their athletic ability and promoting their health.

[0006] Recently, more and more evidence has shown that whey protein plays an important role in improving athletic ability and relieving muscle fatigue after exercise, but whey protein sports drinks are not yet popular on the market. Whey protein is rich in branched-chain amino acids (BCAA) that promote muscle growth and can be used as a nutrient component of sports and fitness functional foods. It plays an important role in improving athletic ability and relieving muscle fatigue after exercise.

[0007] When whey protein is used in sports drinks, the heat resistance of whey protein is poor. It is prone to flocculation, stratification, precipitation, denaturation and other phenomena during the beverage production process and product storage, and has poor stability.

[0008] Therefore, there is an urgent need to provide an electrolyte sports drink containing whey protein with strong stability and good taste. Summary of the invention

[0009] In view of the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a transparent protein electrolyte sports drink and a preparation method thereof, so as to solve the problem of poor stability of whey protein in transparent electrolyte sports drinks in the prior art.

[0010] To achieve the above objectives and other related objectives, the present invention is achieved through the following technical solutions.

[0011] The first aspect of the present invention is to provide a transparent protein electrolyte sports drink, which comprises the following raw materials in weight percentage: 80-85% of nanofiltration filtrate, 4.5-5.5% of sugar, 2.0-3.0% of decolorized and deacidified apple concentrated juice, 1.2-3.2% of whey protein, 0.15-0.25% of acidity regulator, 0.20-0.30% of acai berry extract, 0.20-0.30% of acerola concentrated powder, 0.15-0.20% of natural lemon flavor, and water supplemented to 100%; the acidity regulator is selected from one or more of citric acid or malic acid.

[0012] The second aspect of the present invention is to provide a method for preparing a transparent protein electrolyte sports drink as described above, comprising the following steps:

[0013] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing rate is less than 350 rpm / min;

[0014] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder, and water to obtain a liquid B;

[0015] (3) cooling the liquid A obtained in step (1) and mixing it with the liquid B obtained in step (2) to obtain liquid C;

[0016] (4) The liquid C obtained in step (3) is subjected to steam immersion ultra-high temperature instantaneous sterilization, degassing, cooling, and filling to obtain a transparent protein electrolyte sports drink.

[0017] As described above, the transparent protein electrolyte sports drink and the preparation method thereof of the present invention have the following beneficial effects:

[0018] (1) The present invention provides a method for preparing a transparent protein electrolyte sports drink. By combining milk nanofiltration filtrate, whey protein and acai berry extract, the electrolyte content of the final product is increased by 2 to 3 times compared with traditional sports drinks. The product has the functions of replenishing water, replenishing electrolytes, repairing muscle damage and relieving fatigue.

[0019] (2) The raw materials of the transparent protein electrolyte sports drink of the present invention are all from natural food sources, which are safe and healthy, have little irritation to the human body, and are easily absorbed by the human body;

[0020] (3) The preparation method of the transparent protein electrolyte sports drink of the present invention, the sports drink prepared by ultra-high temperature sterilization is bright transparent pink in color within 6 months at room temperature, the color is attractive, the taste is refreshing without sourness, the flavor is good, and the stability is good, which solves the problems of the stability of whey protein in complex electrolyte solutions and the easy fading of acai berries when heated. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Shown is a product schematic diagram of Example 5 of the present invention.

[0022] Figure 2 Shown is a schematic diagram of the product of Comparative Example 5 of the present invention.

[0023] Figure 3 It is a schematic diagram of the product of comparative example 3 of the present invention which is kept warm for 30 days.

[0024] Figure 4 It is a schematic diagram of the product of comparative example 3 of the present invention which is kept warm for 40 days. DETAILED DESCRIPTION

[0025] In order to make the purpose of the invention, technical solutions and beneficial technical effects of the present invention clearer, the present invention is further described in detail below in conjunction with embodiments. People familiar with this technology can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0026] The first aspect of the present invention is to provide a transparent protein electrolyte sports drink, which comprises the following raw materials in weight percentage: 80-85% of nanofiltration filtrate, 4.5-5.5% of sugar, 2.0-3.0% of decolorized and deacidified apple concentrated juice, 1.2-3.2% of whey protein, 0.15-0.25% of acidity regulator, 0.20-0.30% of acai berry extract, 0.20-0.30% of acerola concentrated powder, 0.15-0.20% of natural lemon flavor, and water supplemented to 100%; the acidity regulator is selected from one or more of citric acid and malic acid.

[0027] The whey protein of the present invention is rich in branched-chain amino acids (BCAA) that have the function of promoting muscle growth, and can be used as a nutrient component of sports and fitness functional food, and plays an important role in improving athletic ability and relieving muscle fatigue after exercise. Whey protein beverage is a sports functional beverage containing whey protein, which is generally transparent or translucent, has high nutritional value, and is easy to absorb. Transparent whey protein beverages are increasingly favored by consumers because of their clear and transparent state;

[0028] The nanofiltration filtrate of the present invention contains a large amount of natural electrolytes such as sodium, potassium, calcium, magnesium, phosphorus and other nutrients required for sports drinks, which can meet the needs of rapid water and electrolyte replenishment;

[0029] During strenuous exercise, a large number of free radicals are generated in the body, leading to oxidative stress, which can damage cells and accelerate aging. The acai berry extract of the present invention is rich in potent antioxidants, which can effectively neutralize these free radicals and reduce oxidative stress, thereby protecting muscles and other cells from damage. However, the anthocyanins in the acai berry are easily affected by heat and light, resulting in the loss of the original bright pink color.

[0030] The present invention facilitates the preparation of a transparent protein electrolyte sports drink that can replenish water, supplement electrolytes, repair muscle damage and relieve fatigue by combining nanofiltration filtrate, whey protein and acai berry extract. The nanofiltration filtrate can replenish water and electrolytes, whey protein can repair muscle damage, and acai berry extract contains potent antioxidants that can eliminate inflammation caused by sports injuries and protect muscles. Therefore, the three main functional ingredients are combined to comprehensively solve the problems of replenishing water, supplementing electrolytes, repairing sports muscle injuries, protecting muscles, and relieving sports fatigue during or after exercise.

[0031] In some embodiments of the present invention, the sports drink comprises the following raw materials in weight percentage: 80-83% nanofiltration filtrate, 4.5-5% sugar, 2.0-2.5% decolorized and deacidified apple concentrated juice, 1.2-2.2% whey protein, 0.15-0.2% acidity regulator, 0.20-0.25% acai berry extract, 0.20-0.25% acerola concentrated powder, 0.15-0.17% natural lemon flavor, and water to 100%.

[0032] In some embodiments of the present invention, the sports drink comprises the following raw materials in weight percentage: 83-85% nanofiltration filtrate, 5-5.5% sugar, 2.5-3.0% decolorized and deacidified apple concentrated juice, 2.2-3.2% whey protein, 0.2-0.25% acidity regulator, 0.25-0.30% acai berry extract, 0.25-0.30% acerola concentrated powder, 0.17-0.20% natural lemon flavor, and water to 100%.

[0033] In some embodiments of the present invention, the sports drink comprises the following raw materials in weight percentage: 80% nanofiltration filtrate, 4.5% sugar, 2.0% decolorized and deacidified apple concentrated juice, 1.2% whey protein, 0.15% acidity regulator, 0.20% acai berry extract, 0.20% acerola concentrated powder, 0.15% natural lemon flavor, and water to 100%.

[0034] In some embodiments of the present invention, the sports drink comprises the following raw materials in weight percentage: 82% nanofiltration filtrate, 5.25% sugar, 2.5% decolorized and deacidified apple concentrated juice, 2.2% whey protein, 0.2% acidity regulator, 0.25% acai berry extract, 0.25% acerola concentrated powder, 0.18% natural lemon flavor, and water to 100%.

[0035] In some embodiments of the present invention, the sports drink comprises the following raw materials in weight percentage: 85% nanofiltration filtrate, 5.5% sugar, 3.0% decolorized and deacidified apple concentrated juice, 3.2% whey protein, 0.25% acidity regulator, 0.30% acai berry extract, 0.30% acerola concentrated powder, 0.20% natural lemon flavor, and water to 100%.

[0036] In some embodiments of the present invention, the nanofiltration filtrate is the filtrate after skimmed raw milk is filtered through a nanofiltration membrane. The present invention has no special requirements for the source of skimmed raw milk, and commercially available products of skimmed raw milk frequently used by those skilled in the art can be used. The pore size of the nanofiltration membrane is 200-400 Daltons, specifically, the pore size can be 200-300 Daltons, 300-400 Daltons; typically but not limiting, for example, 200 Daltons, 300 Daltons, 400 Daltons; the filtration temperature can be 2-6°C, specifically, the temperature can be 2-4°C, 4-6°C; typically but not limiting, for example, 2°C, 4°C, 6°C. The concentration ratio of the nanofiltration membrane is 1.5-2; the potassium content of the nanofiltration filtrate is ≥150mg / 100g, the sodium content is ≥40mg / 100g, the calcium content is ≥30mg / 100g, the magnesium content is ≥8mg / 100g, the phosphorus content is ≥40mg / 100g, and the chloride ion content is ≥100mg / 100g. The nanofiltration filtrate of the present invention is essentially the natural electrolyte component of milk after fat, protein and lactose are removed. Compared with directly drinking beverages with added sodium chloride and potassium chloride electrolytes, the human body has better hydration effect and fatigue relief effect, and the nanofiltration filtrate has richer electrolyte types and a more suitable ratio for the organism.

[0037] In some embodiments of the present invention, the sugar of the present invention is selected from one or more of premium carbonized white granulated sugar and crystallized fructose; the present invention has no particular limitation on the source of premium carbonized white granulated sugar, and a commercial product of premium carbonized white granulated sugar that is often used by a person skilled in the art can be used. The present invention has no particular limitation on the source of crystallized fructose, and a commercial product of crystallized fructose that is often used by a person skilled in the art can be used.

[0038] In some embodiments of the present invention, the decolorized and deacidified apple juice of the present invention can be decolorized and deacidified apple juice commonly used in the art. The present invention has no special limitation on the source of the decolorized and deacidified apple concentrated juice, and the commercially available product of decolorized and deacidified apple juice commonly used by those skilled in the art can be used. Specifically, the soluble solid content of the decolorized and deacidified apple concentrated juice is ≥70%.

[0039] In some embodiments of the present invention, the whey protein of the present invention can be the whey protein commonly used in the art. The present invention has no special limitation on the source of the whey protein, and the commercially available products of the whey protein commonly used by those skilled in the art can be used. Specifically, the whey protein is isolated whey protein, preferably, the protein content of the whey protein is ≥95%, and the pH value of the 1% aqueous solution is 3.5-3.8. Whey protein has astringent feeling. The whey protein used in the present invention is treated with an acidity regulator to maintain a transparent texture, but at the same time, its mouthfeel will also bring sour and astringent feeling. When the added amount reaches a certain level, other flavors (sour, sweet, etc.) are difficult to cover its astringent feeling. Therefore, the amount of whey protein added should be controlled to improve the taste of the beverage.

[0040] In some embodiments of the present invention, the acidity regulator is composed of citric acid and malic acid in a mass ratio of 2-4: 1. Specifically, the mass ratio of citric acid to malic acid can be 2-3: 1, 3-4: 1; typically but not limiting, for example, 2: 1, 3: 1, 4: 1.

[0041] In some embodiments of the present invention, the acai berry extract of the present invention can be the acai berry extract commonly used in the art. The present invention does not specifically limit the source of the acai berry extract, and the commercially available product of the acai berry extract commonly used by those skilled in the art can be used. Specifically, the acai berry extract is a pink instant type, with a total polyphenol content of ≥0.5g / 100g and a total anthocyanin content of 150mg / 100g to 300mg / 100g. When the acai berry extract is added to a sports drink, the color of the acai berry extract is basically unaffected during the preparation of the sports drink, thereby giving the sports drink a bright pink color. Among them, the acai berry extract itself is dark purple, which becomes purple-red when diluted into the solution, and becomes bright pink after ultra-high temperature instantaneous sterilization, and the color is more attractive.

[0042] In some embodiments of the present invention, the acerola cherry concentrated powder of the present invention can be the acerola cherry concentrated powder commonly used in the art. The present invention has no special limitation on the source of the acerola cherry concentrated powder, and the commercially available acerola cherry concentrated powder commonly used by those skilled in the art can be used. Specifically, the acerola cherry concentrated powder is instant, and the vitamin C content is ≥17%.

[0043] In some embodiments of the present invention, the natural lemon flavor can be commercially available or homemade. Specifically, the natural lemon flavor of the present invention is commercially available. The natural lemon flavor contains natural plant ingredients such as lemon oil that are helpful for defoaming, so that the natural lemon flavor has a certain defoaming effect when used, is easy to mix with whey protein, increases the solubility of whey protein, and improves the mixing uniformity between whey protein and other raw materials.

[0044] In some embodiments of the present invention, the water is softened water.

[0045] The second aspect of the present invention is to provide a method for preparing a transparent protein electrolyte sports drink as described above, comprising the following steps:

[0046] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing rate is less than 350 rpm / min;

[0047] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder, and water to obtain a liquid B;

[0048] (3) cooling the liquid A obtained in step (1) and mixing it with the liquid B obtained in step (2) to obtain liquid C;

[0049] (4) The liquid C obtained in step (3) is subjected to steam immersion ultra-high temperature instantaneous sterilization, degassing, cooling, and filling to obtain a transparent protein electrolyte sports drink.

[0050] In some embodiments of the present invention, the mixing method and conditions in step (1) are the mixing methods and conditions commonly used in the art. Specifically, the mixing temperature in step (1) is 40-50° C., the mixing time is 20-30 min, and the mixing stirring rate is 200-300 rpm / min.

[0051] In some embodiments of the present invention, the mixing method and conditions in step (2) are the mixing methods and conditions commonly used in the art. Specifically, the mixing temperature in step (2) is 20-30° C., and the mixing time is 10-20 min.

[0052] In some embodiments of the present invention, the mixing method and conditions in step (3) are the mixing methods and conditions commonly used in the art. Specifically, the mixing temperature in step (3) is 20-30°C; the mixing time is 20-30min; and the mixing stirring rate is 200-300rpm / min.

[0053] In some embodiments of the present invention, the cooling method and conditions in step (3) are the cooling methods and conditions commonly used in the art. Specifically, the cooling temperature in step (3) is 20-30°C.

[0054] In some embodiments of the present invention, the cooling method and conditions in step (4) are the cooling methods and conditions commonly used in the art. Specifically, the cooling temperature in step (4) is 20-30°C.

[0055] In some embodiments of the present invention, the sterilization temperature in step (4) is 140-145°C; the sterilization time is 1-2s. Specifically, the sterilization temperature can be 140-142°C, 142-145°C; typically but not limiting, for example, 140°C, 142°C, 145°C; the sterilization time can be 1-1.5s, 1.5-2s; typically but not limiting, for example, 1s, 1.5s, 2s.

[0056] In some embodiments of the present invention, the degassing in step (4) is performed under vacuum conditions; preferably, the vacuum degree of the degassing is -0.6 to -0.7 bar.

[0057] In some embodiments of the present invention, the filling in step (4) is conventional aseptic filling in the art.

[0058] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0059] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing the specific embodiments rather than for limiting the scope of protection of the present invention; in the present specification and claims, unless otherwise expressly stated herein, the singular forms "a", "an" and "the" include plural forms.

[0060] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those generally understood by those skilled in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to the grasp of the prior art by those skilled in the art and the record of the present invention, any methods, equipment, and materials of the prior art similar or equivalent to the methods, equipment, and materials described in the embodiments of the present invention can also be used to realize the present invention.

[0061] The experimental methods in the following examples without specifying specific conditions were carried out according to conventional methods and conditions, or selected according to the product instructions.

[0062] In the following examples, whey protein was purchased from Shanghai Golden Palm Food Co., Ltd., citric acid and malic acid were purchased from Shanghai Jiecong Trading Co., Ltd., decolorized and deacidified apple concentrate was purchased from Dele Food and Beverage Ingredients Co., Ltd., acai berry extract, acerola cherry concentrate powder and natural lemon flavor were purchased from Brazil Shuanglun Company, and crystalline fructose was purchased from Baolingbao Biological Co., Ltd. The skimmed raw milk was purchased from Bright Dairy Co., Ltd.

[0063] Example

[0064] Table 1 Ratio of raw material components of transparent protein electrolyte sports drink of Examples 1-6

[0065] Raw materials / addition amount (wt%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Nanofiltration filtrate 80 85 80 85 82 83 sugar 5 5.5 4.5 5 5.25 4.75 Decolorized and deacidified apple juice concentrate 2 3 2 3 2.5 2.5 Whey protein 1.2 3.2 3.2 1.2 2.2 2.2 Acidity regulator 0.15 0.25 0.25 0.15 0.2 0.2 Acai Berry Extract 0.2 0.3 0.2 0.3 0.25 0.25 Acerola Concentrate Powder 0.2 0.3 0.2 0.3 0.25 0.25 Natural lemon flavor 0.15 0.2 0.2 0.15 0.17 0.18 water 11.1 2.25 9.45 4.9 7.18 6.67

[0066] Table 2 Technical specifications of raw materials of transparent protein electrolyte sports drink of Examples 1-6

[0067]

[0068]

[0069] Example 1

[0070] The present embodiment provides a transparent protein electrolyte sports drink, the raw material composition ratio is shown in Table 1, the raw material composition performance is shown in Table 2, wherein the sugar is white sugar, and the acidity regulator is citric acid. The preparation method of the nanofiltration filtrate comprises the following steps: filtering the skimmed raw milk through a nanofiltration membrane to obtain a nanofiltration filtrate, wherein the nanofiltration filtrate has a potassium content of ≥150 mg / 100 g, a sodium content of ≥40 mg / 100 g, a calcium content of ≥30 mg / 100 g, a magnesium content of ≥8 mg / 100 g, a phosphorus content of ≥40 mg / 100 g, and a chloride ion content of ≥100 mg / 100 g; the filtration temperature is 5°C, the concentration ratio of the nanofiltration membrane is 1.7; the pore size of the nanofiltration membrane is 300 Daltons.

[0071] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0072] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 40° C., the mixing time is 20 min, and the mixing stirring rate is 200 rpm / min;

[0073] (2) mixing decolorized and deacidified apple juice concentrate, acidity regulator, acai berry extract, acerola cherry concentrate powder and water to obtain liquid B; the mixing temperature is 20° C., and the mixing time is 10 min;

[0074] (3) cooling the liquid A obtained in step (1) to 20° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 20° C., the mixing time is 20 min, and the mixing stirring rate is 200 rpm / min;

[0075] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 140°C, and the sterilization time is 1s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.6 bar; and the cooling temperature is 20°C.

[0076] Example 2

[0077] This embodiment provides a transparent protein electrolyte sports drink, the raw material composition ratio is shown in Table 1, the raw material component properties are shown in Table 2, and the difference from Example 1 is that the raw material composition ratio is different, the acidity regulator is malic acid, and the rest is exactly the same as Example 1.

[0078] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0079] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 50° C., the mixing time is 30 min, and the mixing stirring rate is 300 rpm / min;

[0080] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder and water to obtain a liquid B; the mixing temperature is 30° C., and the mixing time is 20 min;

[0081] (3) cooling the liquid A obtained in step (1) to 30° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 30° C., the mixing time is 30 min, and the mixing stirring rate is 300 rpm / min;

[0082] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 145°C, and the sterilization time is 2s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.7 bar; and the cooling temperature is 30°C.

[0083] Example 3

[0084] This embodiment provides a transparent protein electrolyte sports drink, the raw material component ratio is shown in Table 1, the raw material component properties are shown in Table 2, and the difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, and the acidity regulator is composed of citric acid and malic acid in a mass ratio of 4:1. The rest is exactly the same as Example 1.

[0085] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0086] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 50° C., the mixing time is 20 min, and the mixing stirring rate is 200 rpm / min;

[0087] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder and water to obtain a liquid B; the mixing temperature is 30° C., and the mixing time is 10 min;

[0088] (3) cooling the liquid A obtained in step (1) to 30° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 30° C., the mixing time is 20 min, and the mixing stirring rate is 200 rpm / min;

[0089] (4) sterilizing, degassing, cooling, and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 145°C, and the sterilization time is 1s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.7 bar; and the cooling temperature is 20°C.

[0090] Example 4

[0091] This embodiment provides a transparent protein electrolyte sports drink, the raw material component ratio is shown in Table 1, the raw material component properties are shown in Table 2, and the difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, and the acidity regulator is composed of citric acid and malic acid in a mass ratio of 2:1. The rest is exactly the same as Example 1.

[0092] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0093] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 40° C., the mixing time is 30 min, and the mixing stirring rate is 300 rpm / min;

[0094] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder and water to obtain a liquid B; the mixing temperature is 20° C., and the mixing time is 20 min;

[0095] (3) cooling the liquid A obtained in step (1) to 20° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 20° C., the mixing time is 30 min, and the mixing stirring rate is 300 rpm / min;

[0096] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 140°C, and the sterilization time is 2s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.6 bar; and the cooling temperature is 30°C.

[0097] Example 5

[0098] This embodiment provides a transparent protein electrolyte sports drink, the raw material composition ratio is shown in Table 1, the raw material composition properties are shown in Table 2, and the difference from Example 1 is that the raw material composition ratio is different, the acidity regulator is composed of citric acid and malic acid in a mass ratio of 3:1, and the rest is exactly the same as Example 1.

[0099] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0100] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 45° C., the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0101] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder and water to obtain a liquid B; the mixing temperature is 25° C., and the mixing time is 25 min;

[0102] (3) cooling the liquid A obtained in step (1) to 25° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 25° C., the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0103] (4) The liquid C obtained in step (3) is sterilized, degassed, cooled, and filled to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 142°C, and the sterilization time is 1.5s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.65bar; the cooling temperature is 25°C. The product picture is shown in Figure (1).

[0104] Example 6

[0105] This embodiment provides a transparent protein electrolyte sports drink, the raw material component ratio is shown in Table 1, the raw material component properties are shown in Table 2, and the difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, and the acidity regulator is composed of citric acid and malic acid in a mass ratio of 3:1. The rest is exactly the same as Example 1.

[0106] This embodiment provides a method for preparing a transparent protein electrolyte sports drink, comprising the following steps:

[0107] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing temperature is 45° C., the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0108] (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder and water to obtain a liquid B; the mixing temperature is 25° C., and the mixing time is 25 min;

[0109] (3) cooling the liquid A obtained in step (1) to 25° C. and mixing it with the liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 25° C., the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0110] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 143°C, and the sterilization time is 1.5s; the degassing is carried out under vacuum conditions, the vacuum degree is -0.65bar; and the cooling temperature is 25°C.

[0111] Comparative Example

[0112] Comparative Example 1

[0113] This comparative example provides a method for preparing a transparent protein electrolyte sports drink, which differs from Example 5 in that the natural lemon flavor in step (1) is added in step (2), specifically as follows:

[0114] (1) mixing the nanofiltration filtrate, whey protein and sugar to obtain a feed solution A; the mixing temperature is 45° C., the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0115] (2) mixing decolorized and deacidified apple juice concentrate, acidity regulator, acai berry extract, acerola cherry concentrate powder, natural lemon essence and water to obtain liquid B; the mixing temperature is 25° C., and the mixing time is 25 min;

[0116] Step (3) and step (4) are exactly the same as those in Example 5. The raw materials in step (1) generate a large amount of foam and overflow in the mixing process, thereby affecting the mixed dissolution of whey protein and preventing mass production.

[0117] Comparative Example 2

[0118] This comparative example provides a method for preparing a transparent protein electrolyte sports drink, which is different from Example 5 in that the stirring rate in step (1) is 350 rpm / min, and the rest is exactly the same as Example 5. The stirring rate in step (1) is too high, which causes a large amount of foam to be generated during the mixing process of the raw materials in step (1), resulting in a failure to mix and dissolve the raw materials and the inability to carry out mass production.

[0119] Comparative Example 3

[0120] This comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that no acerola cherry concentrate powder is added, and the rest is exactly the same as Example 5. The color of the product obtained in Comparative Example 3 gradually fades during the shelf life, turning dark yellow after about 3 months and light yellow after about 4 months, and it is difficult to obtain the technical effect of the present invention. The schematic diagram of the product is shown in FIG. Figure 3 and Figure 4 shown.

[0121] Comparative Example 4

[0122] This comparative example provides a method for preparing a transparent protein electrolyte sports drink, which is different from Example 5 in that: the step (4) is not subjected to degassing treatment. Specifically:

[0123] Steps (1) to (3) are exactly the same as in Example 5;

[0124] (4) sterilizing, cooling and filling the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink. The sterilization method is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 143°C, the sterilization time is 1.5s; the cooling temperature is 25°C.

[0125] The product obtained in Comparative Example 4 gradually turned dark yellow in color after 3 months at room temperature, and light yellow after about 5 months, which cannot realize the present invention.

[0126] Comparative Example 5

[0127] This comparative example provides a method for preparing a transparent protein electrolyte sports drink, which differs from Example 5 in that the sterilization method in step (4) is ordinary tubular ultra-high temperature sterilization, and the rest is exactly the same as Example 5. The color of the electrolyte sports drink prepared in this comparative example is dark red. The schematic diagram of the prepared product is as shown in FIG. Figure 2 shown.

[0128] Comparative Example 6

[0129] This comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that the acidity regulator is lactic acid, and the rest is exactly the same as Example 5.

[0130] Comparative Example 7

[0131] This comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that an equal amount of natural grapefruit flavor is used to replace the natural lemon flavor, and the rest is exactly the same as Example 5.

[0132] Comparative Example 8

[0133] This comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that the added amount of whey protein is 4.2%, and the other aspects are exactly the same as Example 5.

[0134] Comparative Example 9

[0135] This comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that no decolorized and deacidified apple concentrated juice is added, and the other aspects are exactly the same as Example 5.

[0136] Performance Testing

[0137] 1. Determination of protein, electrolyte and chlorine content

[0138] (1) Protein determination: The transparent protein electrolyte sports drinks prepared in Examples 1-6 were used to detect the protein content in the final products according to the detection method in GB5009.5-2010 "National Food Safety Standard - Determination of Protein in Foods". The detection results are shown in Table 3.

[0139] (2) Electrolyte determination: The transparent protein electrolyte sports drink prepared in Examples 1-6 was used to detect the electrolyte element content in the final product according to the detection method in GB5009.268-2016 "National Food Safety Standard Determination of Multiple Elements in Food". The detection results are shown in Table 3.

[0140] (3) Determination of chlorine content: The transparent protein electrolyte sports drink prepared in Examples 1-6 was used to detect the chlorine content in the final product according to the detection method in GB5009.44-2016 "National Food Safety Standard Determination of Chloride in Food". The test results are shown in Table 3.

[0141] Table 3 Detection of protein, potassium, sodium, calcium, magnesium, phosphorus and chlorine content in the final products of Examples 1-6

[0142] Item per 100ml Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 protein 1.14g 3.04g 3.04g 1.14g 2.09g 2.09g Potassium 120mg 135mg 122mg 130mg 126mg 125mg sodium 33mg 41mg 34mg 37mg 35mg 36mg calcium 20mg 28mg 23mg 27mg 25mg 26mg magnesium 6.0mg 7.0mg 6.2mg 6.8mg 6.5mg 6.6mg phosphorus 35mg 45mg 39mg 42mg 40mg 41mg chlorine 90mg 97mg 92mg 95mg 93mg 90mg

[0143] It can be seen from the data in Table 3 that the sports drinks prepared in Examples 1-6 have high protein and electrolyte contents per 100 ml, which are 2-3 times that of traditional sports drinks.

[0144] 2. Sensory Evaluation

[0145] The transparent protein electrolyte sports drinks prepared in Examples 1-6 and Comparative Examples 5-9 were subjected to sensory evaluation. Specifically, 30 female professional sensory testers aged 20 to 30 were selected to evaluate the four indicators of color and transparency, sweetness and sourness, taste, and flavor. The total score of the four indicators was used as the sample evaluation score, and the average score of the 30 people was used as the final evaluation score. The scoring rules are shown in Table 4. The experimental results are shown in Record 5.

[0146] Table 4 Sensory evaluation standards

[0147]

[0148] Table 5 Performance test results of transparent protein electrolyte sports drinks of Examples 1-6 and Comparative Examples 5-9

[0149]

[0150]

[0151] Combined with the data in Table 5, it can be seen that the products prepared in Examples 1-6 of the present invention have been well received in terms of color and transparency, sweetness and sourness, astringency, refreshing taste, and flavor. The products are clear and transparent, bright pink, and attractive in color; the taste is refreshing without sourness and astringency, and the sweetness and sourness are moderate; the fresh lemon flavor has no peculiar smell. Comparative Example 5 is dark red and cannot achieve the bright pink color required by the present invention. The sweetness and sourness score of Comparative Example 6, the flavor score of Comparative Example 7, and the taste score of Comparative Example 9 are all lower than the scores of the products prepared in Example 5, and there is also a large gap between the overall scores of Example 5 and Comparative Examples 5-7 and Comparative Example 9. Comparative Example 8 adds more whey protein, and the scores of the products prepared therefrom and the overall score are all poor.

[0152] 3. Stability evaluation

[0153] The transparent protein electrolyte sports drinks prepared in Examples 1-6 and Comparative Examples 3-4 were observed for accelerated shelf life stability. The color and transparency of the products were observed at 37°C. The accelerated 37°C test for 10 days, 20 days, 30 days, 40 days, 50 days and 60 days corresponded to the stability of 1 month, 2 months, 3 months, 4 months, 5 months and 6 months at room temperature (20-25°C). It can be determined that 6 months at room temperature is relatively stable. The results are shown in Table 6.

[0154] Table 6 Transparent protein electrolyte sports drinks prepared in Examples 1-6 and Comparative Examples 3-4

[0155]

[0156]

[0157] Combined with the data in Table 6, it can be seen that the products prepared in Examples 1-6 are clear and transparent and basically maintain a bright pink color after 60 days of the accelerated shelf life test at 37°C. The products prepared in Comparative Examples 3-4 are dark yellow and light yellow after 60 days of the accelerated shelf life test at 37°C.

[0158] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A transparent protein electrolyte sports drink, characterized in that: The sports drink comprises the following raw materials in percentage by weight: 80-85% of nanofiltration filtrate, 4.5-5.5% of sugar, 2.0-3.0% of decolorized and deacidified apple concentrated juice, 1.2-3.2% of whey protein, 0.15-0.25% of acidity regulator, 0.20-0.30% of acai berry extract, 0.20-0.30% of acerola concentrated powder, 0.15-0.20% of natural lemon flavor, and water supplemented to 100%; the acidity regulator is selected from one or more of citric acid or malic acid.

2. A transparent protein electrolyte sports drink according to claim 1, characterized in that: The nanofiltration filtrate is the filtrate obtained after the skimmed raw milk is filtered through a nanofiltration membrane.

3. A transparent protein electrolyte sports drink according to claim 2, characterized in that: Also includes one or more of the following characteristics: A1) the nanofiltration filtrate has a potassium content of ≥150 mg / 100 g, a sodium content of ≥40 mg / 100 g, a calcium content of ≥30 mg / 100 g, a magnesium content of ≥8 mg / 100 g, a phosphorus content of ≥40 mg / 100 g, and a chloride ion content of ≥100 mg / 100 g; A2) The temperature of the nanofiltration membrane filtration is 2-6°C; A3) The pore size of the nanofiltration membrane is 200-400 Daltons.

4. A transparent protein electrolyte sports drink according to claim 1, characterized in that: Also includes one or more of the following characteristics: B1) the sugar is selected from one or more of premium carbonized white sugar or crystalline fructose; B2) the soluble solids content of the decolorized and deacidified apple juice concentrate is ≥ 70%; B3) the whey protein is isolated whey protein, preferably, the protein content of the whey protein is ≥95%, and the pH value of a 1% aqueous solution is 3.5-3.8; B4) the acidity regulator comprises citric acid and malic acid; B5) the total polyphenol content of the acai berry extract is ≥ 0.5 g / 100 g, and the total anthocyanin content is 150 mg / 100 g to 300 mg / 100 g; B6) the vitamin C content of the acerola cherry concentrated powder is ≥ 17%; B7) The water is softened water.

5. A transparent protein electrolyte sports drink according to claim 1, characterized in that: The acidity regulator is composed of citric acid and malic acid in a mass ratio of 2-4:

1.

6. A method for preparing a transparent protein electrolyte sports drink according to any one of claims 1 to 5, characterized in that: The steps include: (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon flavor to obtain liquid A; the mixing rate is less than 350 rpm / min; (2) mixing decolorized and deacidified apple juice concentrate, an acidity regulator, an acai berry extract, acerola cherry concentrate powder, and water to obtain a liquid B; (3) cooling the liquid A obtained in step (1) and mixing it with the liquid B obtained in step (2) to obtain liquid C; (4) The liquid C obtained in step (3) is subjected to steam immersion ultra-high temperature instantaneous sterilization, degassing, cooling, and filling to obtain a transparent protein electrolyte sports drink.

7. The method for preparing a transparent protein electrolyte sports drink according to claim 6, characterized in that: In the step (1), the mixing temperature is 40-50° C., the mixing time is 20-30 min, and the mixing stirring rate is 200-300 rpm / min.

8. The method for preparing a transparent protein electrolyte sports drink according to claim 6, characterized in that: The sterilization temperature in step (4) is 140-145° C.; the sterilization time is 1-2 seconds.

9. The method for preparing a transparent protein electrolyte sports drink according to claim 6, characterized in that: It also includes one or more of the following technical features: 21) The mixing temperature in step (2) is 20-30°C and the mixing time is 10-20min; 31) The mixing temperature in step (3) is 20-30°C; the mixing time is 20-30min; the mixing stirring rate is 200-300rpm / min; 32) The cooling temperature in step (3) is 20-30°C; 41) The cooling temperature in step (4) is 20-30°C.

10. The method for preparing a transparent protein electrolyte sports drink according to claim 6, characterized in that: The degassing in step (4) is carried out under vacuum conditions; preferably, the vacuum degree of the degassing is -0.6 to -0.7 bar.

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