Inonotuso bliquus (Fr) Pilat water-soluble micro powder as well as preparation method

Abstract

The invention relates to the technical field of health care product processing, in particular to inonotuso bliquus (Fr) Pilat water-soluble micro powder as well as a preparation method and application thereof. The invention relates to a preparation method of water-soluble inonotuso bliquus micro powder, which comprises the following steps: preparing an inonotuso bliquus extract by using water as an extracting agent; the preparation method comprises the following steps: mixing an inonotuso bliquus extract with oat beta-glucan, loading the inonotuso bliquus extract with the oat beta-glucan in the mixing process, and drying to obtain the inonotuso bliquus water-soluble micro powder. According to the inonotuso bliquus water-soluble micro powder obtained by the preparation method disclosed by the invention, the solubility of the inonotuso bliquus extract is remarkably improved, the problems that the content of the inonotuso bliquus extract in a terminal product is low and the pharmacological action is difficult to give full play are solved, the stability of the inonotuso bliquus extract is enhanced, and the application range of the inonotuso bliquus extract in the fields of food and health care is expanded.

Description

Technical Field

[0001] The invention relates to the technical field of health care product processing, and in particular to a chaga water-soluble micropowder and a preparation method and application thereof. Background Art

[0002] Chaga is a rare medicinal fungus that grows in cold regions, especially in Siberia, Russia and Northeast my country. It is not only a folk medicine with a long history, but also attracts much attention in the field of modern medicine and health products because of its rich pharmacologically active ingredients and wide range of health benefits. The main chemical components of Chaga are extremely complex and diverse, including but not limited to polysaccharides, betulin, betulinol, a series of oxidized triterpenoids, traemonic acid, a variety of lanosterol-type triterpenoids, folic acid derivatives, and aromatic vanillic acid, syringic acid and γ-hydroxybenzoic acid. These ingredients together give Chaga multiple pharmacological activities such as anti-tumor, enhancing immunity, regulating blood sugar levels, lowering blood pressure, anti-oxidation, inhibiting the replication of infectious viruses, and improving nervous system function, showing extremely high health and therapeutic value.

[0003] With the rapid development of modern science and technology, especially biotechnology and pharmaceutical technology, the research on Chaga has been deepened from traditional macroscopic observation to the molecular level, and its extraction and application technology has also made significant progress. Existing research has effectively improved the extraction efficiency and quality of effective ingredients in Chaga by optimizing the extraction process, such as using advanced technologies such as ultrasonic extraction, microwave-assisted extraction, and supercritical fluid extraction. However, Chaga extracts prepared by traditional methods often exist in the form of powders or oils with poor solubility. These forms not only have poor solubility, but also when prepared into end products, the effective ingredients are easily degraded or lost during the processing process, resulting in insufficient content of Chaga extract in the final product, and unable to fully exert its pharmacological effects. In addition, due to poor solubility, consumers often find it difficult to form a stable and easily absorbed solution when using these extracts, which affects the user experience, thereby limiting the wide application and in-depth development of Chaga extracts in the fields of food and health care. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention provides a Chaga water-soluble micropowder and a preparation method and application thereof. The present invention uses water as an extractant to prepare a Chaga extract; then the Chaga extract is mixed with oat β-glucan, during which the oat β-glucan loads the Chaga extract to form a stable complex, and then the Chaga water-soluble micropowder is obtained through drying. The Chaga water-soluble micropowder obtained by the preparation method of the present invention not only significantly improves the solubility of the Chaga extract, effectively reduces the degradation and loss of the Chaga extract during processing, but also enhances the stability of the Chaga extract, and expands its application range in the fields of food and health care.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] The first object of the present invention is to provide a method for preparing chaga water-soluble micropowder, comprising the following steps:

[0007] The Chaga extract is mixed with oat β-glucan. During the mixing process, the oat β-glucan loads the Chaga extract, and then the mixture is dried to obtain Chaga water-soluble micropowder. Among them, oat β-glucan is a high-viscosity soluble dietary fiber with excellent water solubility. It can significantly improve the solubility of the Chaga extract and enhance the bioabsorbability of the Chaga water-soluble micropowder through a synergistic effect.

[0008] Preferably, the mass ratio of Chaga extract to oat β-glucan is 1:0.15-0.25; if the ratio of oat β-glucan is low, the dissolution rate of Chaga water-soluble micropowder will be slowed down, affecting the use effect of Chaga water-soluble micropowder.

[0009] Preferably, the drying treatment adopts spray drying, the inlet air temperature of the spray drying is 160℃~180℃, the outlet air temperature is 90℃~110℃, and the feed pressure is 0.06MPa~0.12Mpa; wherein, during the spray drying process, the water in the complex formed by the Chaga extract and the oat β-glucan can evaporate rapidly, thereby realizing the direct conversion of the solution into the Chaga water-soluble micropowder, and this conversion process omits the intermediate steps of evaporation and crushing, further simplifies the process flow, and improves the production efficiency; and the core of the drying efficiency lies in the boiling point characteristics of the solvent, and since the solvent used in the present invention is water, its boiling point is about 100℃. Therefore, the outlet air temperature is set at 90℃~110℃, aiming to ensure the effective evaporation of water without causing overheating damage to the Chaga water-soluble micropowder; at the same time, in order to ensure the energy efficiency and stability of the drying process, the temperature difference between the inlet air temperature and the outlet air temperature cannot be too small, so as to avoid a surge in air demand caused by a small temperature difference, thereby causing an unnecessary increase in energy consumption and heat energy costs.

[0010] Preferably, a concentration treatment is performed before the drying treatment, and the concentration treatment is to reduce the drying time.

[0011] Preferably, the conditions for the concentration treatment are: reducing the pressure to -0.09 MPa to -0.1 MPa at 60° C. to 70° C., and concentrating for 1 h to 7 h until the solid content is 30% to 50%.

[0012] Preferably, when preparing the Chaga extract, Chaga and water are mixed and then extracted; wherein the mass ratio of Chaga to water is 1:6-10; wherein, if the water content is too low, the extraction will be incomplete and the effect will be affected; conversely, if the water content is too high, the subsequent spray drying process will be prolonged and the energy consumption will be increased.

[0013] Preferably, the Chaga is pulverized before being mixed with water, and the particle size is 10-30 meshes. Too large particles will lead to incomplete extraction, affecting the quality and efficacy of the Chaga water-soluble micropowder.

[0014] Preferably, the extraction conditions are: soaking for 25 min to 35 min at 110° C. to 130° C. and 0.1 MPa to 0.3 MPa.

[0015] The second object of the present invention is to provide the Chaga water-soluble fine powder prepared by the above preparation method.

[0016] The third object of the present invention is to provide the use of the above-mentioned Chaga water-soluble micropowder in the preparation of food or health care product additives.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The present invention provides a method for preparing Chaga water-soluble micropowder, wherein Chaga extract and oat β-glucan are mixed, during the mixing process, oat β-glucan loads Chaga extract to form a stable complex, and then the Chaga water-soluble micropowder is obtained by drying. The Chaga water-soluble micropowder obtained by the preparation method of the present invention not only significantly improves the solubility of Chaga extract, making its dispersibility in various application systems more superior, but also greatly enhances the bioabsorbability of Chaga water-soluble micropowder through the synergistic effect of oat β-glucan, so that its nutritional and health value can be more fully and quickly utilized by the human body.

[0019] Among them, β-glucan is a type of non-starch polysaccharide formed by connecting glucose structural units with β-glycosidic bonds. It is widely found in higher plants, large fungi, algae, yeast and bacterial organisms. Compared with α-glucan, oat β-glucan has good water solubility and biocompatibility, and can form a stable complex with Chaga extract, thereby improving the solubility and stability of the entire system. In addition, oat β-glucan particles present a unique hollow structure and a porous structure on the surface. This structure can carry large doses of Chaga extract, which not only significantly enhances the water solubility of Chaga extract, but also makes Chaga extract show a sustained release effect during the release process, prolonging its action time in the body, thereby further improving the utilization rate of its nutritional and health value.

[0020] 2. The present invention provides a Chaga water-soluble micropowder with health-care function. The Chaga water-soluble micropowder has a strong and lasting Chaga flavor, a pure taste, good water-soluble effect, and inherits the multiple biological active functions of Chaga itself, and has significant hypoglycemic, anti-inflammatory, antioxidant and anti-tumor effects.

[0021] Among them, Chaga polysaccharide, as one of the main active ingredients of Chaga, has been effectively retained and enhanced in Chaga water-soluble micropowder. Chaga polysaccharide can inhibit the growth of a variety of tumor cells, including melanoma, S180 sarcoma, glioma, liver cancer and ovarian cancer, thereby showing strong anti-tumor activity in vivo and in vitro. In addition, Chaga polysaccharide can also repair insulin β cells and promote insulin secretion, thereby achieving the effect of lowering blood sugar, and its effect is similar to that of the commonly used hypoglycemic drug metformin. At the same time, Chaga polysaccharide can also promote the transport and metabolism of cholesterol, inhibit the intestinal absorption of cholesterol, improve endothelial function, and reduce free radical damage, thereby exerting excellent antioxidant and cardiovascular protection effects.

[0022] In addition, the addition of β-glucan further enhances the health function of Chaga water-soluble micropowder. β-glucan not only has multiple biological functions of anti-oxidation, immunomodulation, anti-tumor, and hypoglycemic, but also can produce a synergistic effect with Chaga polysaccharides to enhance the biological activity of Chaga water-soluble micropowder. β-glucan can enhance the activity of intracellular antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, and catalase, thereby reducing the levels of oxygen free radicals and oxidative stress products and protecting cells from oxidative damage. Therefore, the Chaga water-soluble micropowder not only has a unique flavor and good water solubility, but also shows excellent health benefits in hypoglycemic, anti-inflammatory, anti-oxidation and anti-tumor, providing strong support for the development of new foods, health products and medicines. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a raw material diagram of Chaga in the present invention.

[0024] Figure 2 The present invention is a diagram of the Chaga water-soluble fine powder obtained in Example 2. DETAILED DESCRIPTION

[0025] The technical solution of the present invention will be clearly and completely described below in combination with the data in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] It should be noted that the professional terms used in the present invention are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention. Unless otherwise specifically stated, the various raw materials, reagents, instruments and equipment used in the following embodiments of the present invention can be purchased from the market or prepared by existing methods.

[0027] Among them, Chaga was purchased from Suifenhe Shida Import and Export Co., Ltd., and oat β-glucan was purchased from Zhejiang Shenyou Biotechnology Co., Ltd.

[0028] In the prior art, although a variety of advanced extraction technologies have been used to improve the extraction efficiency and quality of the effective ingredients in Chaga, how to effectively improve the solubility of Chaga extracts in applications and reduce the loss of effective ingredients during processing is still a technical problem that needs to be solved urgently. Traditional Chaga extracts are often in the form of powders or oils. These forms not only have poor solubility, but also when they are prepared into end products, the effective ingredients are easily degraded or lost during processing, resulting in insufficient Chaga extract content in the final product, and unable to fully exert its pharmacological effects. In addition, due to poor solubility, consumers often find it difficult to form a stable and easily absorbed solution when using these extracts, which affects the user experience, thereby limiting the wide application and in-depth development of Chaga extracts in the fields of food and health care.

[0029] In view of the problems existing in the prior art, the present invention provides a method for preparing a chaga mushroom water-soluble micropowder, comprising the following steps: using water as an extractant to prepare a chaga mushroom extract; mixing the chaga mushroom extract with oat β-glucan, during the mixing process, the oat β-glucan loads the chaga mushroom extract, and then drying to obtain the chaga mushroom water-soluble micropowder.

[0030] Among them, oat β-glucan has good water solubility and biocompatibility, and can form a stable complex with Chaga extract, thereby improving the solubility and stability of the entire system. In addition, oat β-glucan particles present a unique hollow structure and a porous structure on the surface. This structure can carry a large dose of Chaga extract, which not only significantly enhances the water solubility of Chaga extract, but also makes Chaga extract show a sustained release effect during the release process, prolonging its action time in the body, thereby further improving the utilization rate of its nutritional and health value.

[0031] The technical solution of the present invention is further explained by using embodiments below, which are specifically as follows:

[0032] Example 1

[0033] A method for preparing chaga water-soluble micropowder comprises the following steps:

[0034] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 10-mesh sieve to obtain crushed Chaga.

[0035] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0036] S3. Add 1 kg of oat β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 7 hours at 60°C by reducing the pressure to -0.09 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 160°C and the outlet air temperature to 90°C, and ensure that the inlet air pressure is maintained at 0.06 MPa. Spray drying is carried out under this condition to obtain Chaga water-soluble micropowder.

[0037] Example 2

[0038] A method for preparing chaga water-soluble micropowder is the same as the method of Example 1, except that the amount of oat β-glucan added in S3 is replaced by 2 kg from 1 kg, comprising the following steps:

[0039] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 10-mesh sieve to obtain crushed Chaga.

[0040] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0041] S3. Add 2 kg of oat β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 7 hours at 60°C by reducing the pressure to -0.09 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 160°C and the outlet air temperature to 90°C, and ensure that the inlet air pressure is maintained at 0.06 MPa. Spray drying is carried out under these conditions to obtain Chaga water-soluble micropowder.

[0042] Example 3

[0043] A method for preparing chaga water-soluble micropowder is the same as the method of Example 1, except that the amount of oat β-glucan added in S3 is replaced by 3 kg from 1 kg, comprising the following steps:

[0044] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 10-mesh sieve to obtain crushed Chaga.

[0045] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0046] S3. Add 3 kg of oat β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 1 hour at 70°C by reducing the pressure to -0.1 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 180°C and the outlet air temperature to 110°C, and ensure that the inlet air pressure is maintained at 0.12 MPa. Spray drying is carried out under these conditions to obtain Chaga water-soluble micropowder.

[0047] Example 4

[0048] A method for preparing water-soluble Chaga powder is the same as the method in Example 1, except that the 10-mesh sieve of crushed Chaga in S1 is replaced by a 30-mesh sieve, comprising the following steps:

[0049] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 30-mesh sieve to obtain crushed Chaga.

[0050] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0051] S3. Add 1 kg of oat β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 7 hours at 60°C by reducing the pressure to -0.09 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 160°C and the outlet air temperature to 90°C, and ensure that the inlet air pressure is maintained at 0.06 MPa. Spray drying is carried out under this condition to obtain Chaga water-soluble micropowder.

[0052] Example 5

[0053] A method for preparing water-soluble Chaga powder is the same as the method in Example 1, except that the 10-mesh sieve of crushed Chaga in S3 is replaced by a 30-mesh sieve, comprising the following steps:

[0054] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 30-mesh sieve to obtain crushed Chaga.

[0055] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0056] S3. Add 1 kg of oat β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 7 hours at 60°C by reducing the pressure to -0.09 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 160°C and the outlet air temperature to 90°C, and ensure that the inlet air pressure is maintained at 0.06 MPa. Spray drying is carried out under this condition to obtain Chaga water-soluble micropowder.

[0057] Comparative Example 1

[0058] A method for preparing Chaga agaricus powder is the same as the method in Example 1, except that oat β-glucan is not added in S3, and comprises the following steps:

[0059] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 10-mesh sieve to obtain crushed Chaga.

[0060] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0061] S3. Spray-dry the Chaga extract at 130° C. to obtain Chaga fine powder.

[0062] Comparative Example 2

[0063] A method for preparing chaga water-soluble micropowder is the same as the method of Example 1, except that oat β-glucan is replaced by yeast β-glucan, comprising the following steps:

[0064] S1. Select Chaga, remove impurities, select the undamaged and undeteriorated parts, crush them and pass them through a 10-mesh sieve to obtain crushed Chaga.

[0065] S2. Weigh 1 kg of crushed Chaga and 10 L of water respectively, mix the crushed Chaga and water, put them into an autoclave, and sterilize them at 121° C. and 0.3 MPa for 30 min to obtain a Chaga extract.

[0066] S3. Add 2 kg of yeast β-glucan to the Chaga extract and mix them thoroughly. Then, use a rotary evaporator to concentrate the mixture for 7 hours at 60°C by reducing the pressure to -0.09 MPa. After concentration, adjust the spray drying parameters, set the inlet air temperature to 160°C and the outlet air temperature to 90°C, and ensure that the inlet air pressure is maintained at 0.06 MPa. Spray drying is carried out under these conditions to obtain Chaga water-soluble micropowder.

[0067] Among them, yeast β-glucan has poor water solubility. After being added to the Chaga extract, some precipitation occurs, which affects the appearance and taste of the Chaga water-soluble micropowder.

[0068] Performance Test:

[0069] Taking the Chaga water-soluble powder prepared in Example 2 of the present invention and the Chaga powder prepared in Comparative Example 1 as examples, the total flavonoids were measured using the Al(NO3)3-NaNO2-NaOH colorimetric method. The specific method and results are as follows:

[0070] 0.5 g of the Chaga water-soluble powder prepared in Example 2 and 0.5 g of the Chaga powder prepared in Comparative Example 1 were mixed with 0.4 mL of a 5% NaNO2 solution and transferred to a 10 mL stoppered test tube; after being fully mixed and allowed to stand for 6 min, 0.4 mL of a 10% Al(NO3)3 solution was added, mixed again and allowed to stand for 6 min; then 4.0 mL of a 1 mol / L NaOH solution was added to each test tube, and the volume was adjusted with distilled water, mixed evenly and allowed to stand for 15 min; finally, the absorbance value was detected at a wavelength of 514 nm. The results showed that the dissolution rate of flavonoids in the Chaga agaric powder prepared in Comparative Example 1 was 75.26%, while the dissolution rate of flavonoids in the Chaga agaric water-soluble powder prepared in Example 2 was 89.15%. Compared with the Chaga agaric powder without the addition of oat β-glucan in Comparative Example 1, the dissolution rate of flavonoids in the Chaga agaric water-soluble powder prepared in Example 2 was increased by 18.45%.

[0071] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0072] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing chaga water-soluble micropowder, characterized in that: The following steps are involved: Using water as an extractant to prepare Chaga extract; The Chaga extract and oat beta-glucan are mixed. During the mixing process, the oat beta-glucan loads the Chaga extract. The mixture is then dried to obtain Chaga water-soluble micropowder.

2. The method for preparing a water-soluble Chaga micropowder according to claim 1, characterized in that: The mass ratio of the Chaga extract to oat beta-glucan is 1:0.15-0.

25.

3. The method for preparing a water-soluble Chaga micropowder according to claim 1, characterized in that: The drying process is spray drying.

4. The method for preparing a water-soluble chaga powder according to claim 3, wherein the inlet air temperature of the spray drying is 160°C to 180°C, the outlet air temperature is 90°C to 110°C, and the feed pressure is 0.06MPa to 0.12MPa.

5. The method for preparing a water-soluble Chaga micropowder according to claim 1, characterized in that: A concentration process was also performed before the drying process.

6. The method for preparing a water-soluble Chaga micropowder according to claim 1, characterized in that: When preparing the Chaga extract, Chaga and water are mixed and then extracted; wherein the mass ratio of Chaga to water is 1:6-10.

7. The method for preparing a water-soluble Chaga micropowder according to claim 6, characterized in that: The extraction conditions are: soaking for 25min to 35min at 110°C to 130°C and 0.1MPa to 0.3MPa.

8. Chaga water-soluble micropowder obtained by the preparation method according to any one of claims 1 to 7.

9. Use of the Chaga water-soluble micropowder according to claim 8 in preparing food or health product additives.

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