Bionic sebum composition, skin care product containing bionic sebum composition and application of bionic sebum composition

Abstract

The invention discloses a bionic sebum composition, a skin care product containing the bionic sebum composition and application of the bionic sebum composition. The bionic fetal sebum composition is prepared from the following components in parts by mass: 0.01 to 10 parts of macadamia nut oil, 0.01 to 10 parts of egg yolk oil and 0.01 to 10 parts of HPID. The skin care product containing the bionic fetal sebum composition can have excellent moisturizing performance and skin barrier stability at the same time.

Description

Technical Field

[0001] The invention relates to a bionic fetal skin fat composition, a skin care product containing the bionic fetal skin fat composition and application thereof. Background Art

[0002] With regard to human skin, it is generally accepted that the water barrier of the skin actually improves with age, as measured by transepidermal water loss or permeability to tritium water. However, after injury, the recovery of barrier function is impaired. In aged skin, the lipid content of the stratum corneum decreases compared to young skin. Sebum is composed of squalene, wax esters, cholesterol esters, and triglycerides. On the surface of the skin, some triglycerides are hydrolyzed to release fatty acids, some of which have antimicrobial properties. Sebaceous glands are highly active in utero under the influence of maternal hormones, and sebum contributes significantly to fetal sebum, with the secretion rate of sebum peaking in the teenage years and declining thereafter. People over about sixty-five years of age have very low sebum secretion rates. The reduction in sebum secretion and the simultaneous reduction in antimicrobial fatty acids on the skin surface contribute to an increased incidence of skin infections in the elderly.

[0003] The skin of infants and children is delicate, with abundant subcutaneous blood vessels. The stratum corneum on the skin surface has not yet fully formed, the dermis is thin, with less fibrous tissue, and a well-developed capillary network. After the fetus is born from the mother's body, it is immersed in amniotic fluid and comes to a dry environment. Some slight stimuli, such as clothing and medicines, often cause the baby's skin to become congested, resulting in multiform erythema of varying sizes and unclear edges.

[0004] The sebum membrane is a protective film formed on the skin surface by the low-temperature emulsification of oil secreted from the sebaceous glands, lipids produced by keratinocytes, sweat secreted from the sweat glands, and shed keratinocytes. The total amount of sebum in infants and children is low, which means that infant skin is more fragile than that of adults, has lower resistance to infection, poorer moisturizing function, and more easily damaged skin barrier. When choosing infant skin care products, you should choose skin care products that are more moisturizing and can stabilize the skin barrier.

[0005] Therefore, based on the characteristics of infant skin, it is urgent to design a streamlined natural composition to effectively and specifically address the problems of sensitive and fragile skin caused by the thin stratum corneum, fragile skin barrier, and poor water retention and moisture locking ability of infants and children. Summary of the invention

[0006] In order to overcome the above defects of the prior art, the present invention provides a bionic fetal skin fat composition, a skin care product containing the bionic fetal skin fat composition and its application. The skin care product containing the bionic fetal skin fat composition has excellent moisturizing properties and can also stabilize the skin barrier.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0008] The first aspect of the present invention provides a bionic fetal skin fat composition, which comprises the following components in parts by mass:

[0009] 0.01-10 parts macadamia nut oil, 0.01-10 parts egg yolk oil and 0.01-10 parts HPID (phytosteryl isostearyl dimer dimer linoleate).

[0010] The present invention proposes for the first time to use a specific combination of macadamia oil, egg yolk oil and HPID to prepare bionic fetal skin fat with excellent performance. Among them, the applicant's research found that the two components of macadamia oil and egg yolk oil have extremely high sebum similarity, but when the two are only compounded to make subsequent skin care products, the effect on moisturizing and stabilizing the skin barrier is still lacking; and after a large number of attempts, it was found that even if other components with sebum similarity were further added, there was still no obvious improvement in the effect. However, in this process, the applicant unexpectedly discovered that the HPID component, from the perspective of physical and chemical parameters, is similar to the chemical structure of intercellular lipids and has a similar composition ratio; from the perspective of performance parameters, this component has excellent oxidative stability and is not easy to rancid; after introducing it into the combination of macadamia oil and egg yolk oil, it combines the sebum similarity and intercellular lipid similarity, and achieves a significantly better synergistic effect than the combination of the two, while promoting the effects of moisturizing and stabilizing the skin barrier.

[0011] In some embodiments, in the biomimetic fetal skin fat composition, the mass fraction of the macadamia nut oil is 0.03-10 parts, such as 1 or 5 parts.

[0012] In some embodiments, in the bionic fetal skin fat composition, the mass fraction of the egg yolk oil is 0.03-5 parts, such as 0.16 parts or 1 part.

[0013] In some embodiments, in the biomimetic fetal skin fat composition, the mass fraction of the HPID is 0.03-10 parts, such as 0.16 parts, 1 part or 5 parts.

[0014] In some preferred embodiments, the biomimetic fetal skin fat composition includes: 0.03-10 parts of macadamia nut oil, 0.03-5 parts of egg yolk oil and 0.03-10 parts of HPID.

[0015] In some specific embodiments, the biomimetic fetal skin fat composition includes: 0.03 parts of macadamia nut oil, 0.03 parts of egg yolk oil and 0.03 parts of HPID.

[0016] In some specific embodiments, the biomimetic fetal skin fat composition includes: 1 part of macadamia nut oil, 1 part of egg yolk oil and 5 parts of HPID.

[0017] In some specific embodiments, the biomimetic fetal skin fat composition includes: 1 part of macadamia nut oil, 1 part of egg yolk oil and 10 parts of HPID.

[0018] In some specific embodiments, the biomimetic fetal skin fat composition includes: 0.16 parts of macadamia nut oil, 0.16 parts of egg yolk oil and 0.16 parts of HPID.

[0019] In some specific embodiments, the biomimetic fetal skin fat composition includes: 5 parts of macadamia nut oil, 1 part of egg yolk oil and 1 part of HPID.

[0020] In some specific embodiments, the biomimetic fetal skin fat composition includes: 10 parts of macadamia nut oil, 5 parts of egg yolk oil and 1 part of HPID.

[0021] In some embodiments, the macadamia oil includes 12 fatty acids, of which unsaturated fatty acids (UFAs) account for more than 83% of the total fatty acid content, including palmitoleic acid (C16:1), oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), arachidic acid (C20:1) and docosenoic acid (C22:1); among the unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs) account for 96.67%-98.26%, mainly composed of C18:1 (accounting for 61.74%-66.47% of the total fatty acids) and C16:1 (accounting for 13.22%-17.63% of the total fatty acids).

[0022] In some embodiments, the macadamia oil includes one or more of free fatty acids, glyceride compounds, ceramide compounds, and phospholipid compounds.

[0023] The glyceride compounds preferably include one or more of monosaccharide diglyceride, disaccharide diglyceride, diglyceride, triglyceride, cholesterol ester and hemi-monophosphate glyceride.

[0024] Wherein, the ceramide compound preferably includes one or more of ceramide, dihydroceramide and hexosylceramide.

[0025] The phospholipid compounds preferably include one or more of sphingomyelin, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine.

[0026] In some preferred embodiments, the macadamia nut oil includes one or more of free fatty acids, monosaccharide diglycerides, disaccharide diglycerides, diglycerides, triglycerides, cholesterol esters, hemimonophosphoglycerides, ceramides, dihydroceramides, hexosylceramides, sphingomyelins, lysophosphatidylethanolamines, lysophosphatidylglycerols, lysophosphatidylserines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols, and phosphatidylserines.

[0027] In some more preferred embodiments, the macadamia oil includes the following components by mass percentage: 0-8% free fatty acids, 0-5% monosaccharide diglycerides, 0-5% disaccharide diglycerides, 0-5% diglycerides, 90-100% triglycerides, 0-5% cholesterol esters, 0-5% ceramides, 0-5% dihydroceramides, 0-5% hexosylceramides, 0-5% sphingomyelins, 0-5% lysophosphatidylethanolamines, 0-5% lysophosphatidylglycerols, 0-5% lysophosphatidylserines, 0-5% phosphatidylcholine, 0-5% phosphatidylethanolamines, 0-5% phosphatidylglycerols, 0-5% phosphatidylinositols, 0-5% phosphatidylserines, and 0-5% hemi-monophosphoglycerides.

[0028] In some specific embodiments, the macadamia oil comprises: 27.001 μg / mL free fatty acids, 0.692 μg / mL monosaccharide diglycerides, 0.0004 μg / mL disaccharide diglycerides, 3.220 μg / mL diglycerides, 739.742 μg / mL triglycerides, 2.382 μg / mL cholesterol esters, 0.001 μg / mL ceramides, 0.006 μg / mL dihydroceramides, 0.002 μg / mL hexosylceramides, 0.176 μg / mL sphingomyelin, 0.002 μg / mL lysophosphatidylglycerol, 0.011 μg / mL lysophosphatidylserine, 2.321 μg / mL phosphatidylcholine, 1.414 μg / mL phosphatidylethanolamine, 0.196 μg / mL phosphatidylglycerol, 0.490 μg / mL phosphatidylinositol, 0.344 μg / mL phosphatidylserine, and 0.206 μg / mL hemi-monophosphoglyceride.

[0029] In some embodiments, the macadamia nut oil can be obtained by conventional preparation methods in the art, such as pressing method, solvent method or aqueous method; preferably, it is prepared by aqueous method.

[0030] In some embodiments, the macadamia oil has an acid value of 0.40-1.10 mg KOH / g, such as 0.43 mg KOH / g, 0.68 mg KOH / g or 1.07 mg KOH / g.

[0031] In some embodiments, the macadamia nut oil has a hydrogen peroxide value of 0.10-0.50 mg KOH / g, such as 0.2 mg KOH / g, 0.32 mg KOH / g or 0.44 mg KOH / g.

[0032] In some embodiments, the water and volatile matter content of the macadamia nut oil is 0.01-0.70%, for example 0.45% or 0.62%.

[0033] In some embodiments, the refractive index of the macadamia oil is 1.40-1.60, for example 1.50, 1.56 or 1.57.

[0034] In some embodiments, the iodine value of the macadamia nut oil is 68.00-72.00 gI 2 / 100g, for example 69.19g 2 / 100g、70.21gI 2 / 100g or 71.31 gI 2 / 100g.

[0035] In some embodiments, the macadamia nut oil has a saponification value of 185.00-192.00 mg KOH / g, for example 189.01 mg KOH / g, 189.36 mg KOH / g or 190.13 mg KOH / g.

[0036] In some preferred embodiments, the macadamia nut oil has an acid value of 1.07 mg KOH / g, a hydrogen peroxide value of 0.2 mg KOH / g, a moisture and volatile matter content of 0.01%, a refractive index of 1.56, and an iodine value of 71.31 gI 2 / 100g, saponification value is 190.13 mg KOH / g.

[0037] In some preferred embodiments, the macadamia nut oil has an acid value of 0.68 mg KOH / g, a hydrogen peroxide value of 0.32 mg KOH / g, a moisture and volatile matter content of 0.62%, a refractive index of 1.57, and an iodine value of 70.21 gI 2 / 100g, saponification value is 189.01 mg KOH / g.

[0038] In some more preferred embodiments, the macadamia nut oil has an acid value of 0.43 mg KOH / g, a hydrogen peroxide value of 0.44 mg KOH / g, a moisture and volatile matter content of 0.45%, a refractive index of 1.50, and an iodine value of 69.19 gI 2 / 100g, saponification value is 189.36 mg KOH / g.

[0039] In some embodiments, the egg yolk oil includes triglycerides, and the fatty acid composition of the triglycerides is 42.86% oleic acid, 22.95% linoleic acid, 19.91% palmitic acid, 7.89% stearic acid, 2.25% palmitoleic acid and 0.94% linolenic acid.

[0040] In some embodiments, the egg yolk oil includes: one or more of free fatty acids, glyceride compounds, ceramide compounds and phospholipid compounds.

[0041] The glyceride compounds preferably include one or more of monosaccharide diglycerides, disaccharide diglycerides, diglycerides, triglycerides and cholesterol esters.

[0042] Wherein, the ceramide compound preferably includes one or more of ceramide, dihydroceramide and hexosylceramide.

[0043] The phospholipid compounds preferably include one or more of sphingomyelin, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine.

[0044] In some preferred embodiments, the egg yolk oil includes one or more of free fatty acids, monosaccharide diglycerides, disaccharide diglycerides, diglycerides, triglycerides, cholesterol esters, ceramides, dihydroceramides, hexosylceramides, sphingomyelins, lysophosphatidylethanolamines, lysophosphatidylglycerols, lysophosphatidylserines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols, and phosphatidylserines.

[0045] In some more preferred embodiments, the egg yolk oil includes the following components in mass percentage: 0-7% free fatty acids, 0-5% monosaccharide diglycerides, 0-5% disaccharide diglycerides, 34-54% diglycerides, 46-56% triglycerides, 3-13% cholesterol esters, 0-5% ceramides, 0-5% dihydroceramides, 0-5% hexosylceramides, 0-5% lysophosphatidylethanolamine, 0-5% lysophosphatidylserine, 0-5% phosphatidylcholine, 0-5% phosphatidylethanolamine, 0-5% phosphatidylglycerol, 0-5% phosphatidylinositol and 0-5% phosphatidylserine.

[0046] In some specific embodiments, the egg yolk oil comprises: 50.111 μg / mL free fatty acids, 0.692 μg / mL monosaccharide diglyceride, 0.182 μg / mL disaccharide diglyceride, 1074.697 μg / mL diglyceride, 1392.533 μg / mL triglyceride, 205.084 cholesterol ester, 0.0619 μg / mL ceramide, 0.001 μg / mL dihydroceramide, 0.003 hexosylceramide, 0.02 μg / mL lysophosphatidylethanolamine, 0.011 μg / mL lysophosphatidylserine, 4.946 μg / mL phosphatidylcholine, 1.707 μg / mL phosphatidylethanolamine, 1.132 μg / mL phosphatidylglycerol, 0.403 μg / mL phosphatidylinositol, 0.267 μg / mL phosphatidylserine and 1.136 μg / mL hemi-monophosphoglyceride.

[0047] In some embodiments, the egg yolk oil can be obtained by conventional preparation methods in the art, such as high temperature cooking method, solvent method or supercritical method; preferably, it is prepared by supercritical method.

[0048] In some embodiments, the egg yolk oil has an acid value of 0.10-3.70 mg KOH / g, such as 0.13 mg KOH / g, 1.69 mg KOH / g, or 3.65 mg KOH / g.

[0049] In some embodiments, the egg yolk oil has a hydrogen peroxide value of 0.40-2.20 mg KOH / g, such as 0.44 mg KOH / g, 1.42 mg KOH / g, or 2.19 mg KOH / g.

[0050] In some embodiments, the egg yolk oil has a moisture and volatile matter content of 0.40-1.10%, such as 0.45%, 0.70% or 1.09%.

[0051] In some embodiments, the egg yolk oil has a refractive index of 1.40-1.60, such as 1.50, 1.56 or 1.57.

[0052] In some embodiments, the iodine value of the egg yolk oil is 69.00-71.00 gI 2 / 100g, for example 69.19 g 2 / 100g、70.01gI 2 / 100g or 70.31 gI 2 / 100g.

[0053] In some embodiments, the egg yolk oil has a saponification value of 188.00-193.00 mg KOH / g, such as 189.31 mg KOH / g, 189.36 mg KOH / g, or 192.13 mg KOH / g.

[0054] In some preferred embodiments, the egg yolk oil has an acid value of 3.65 mg KOH / g, a hydrogen peroxide value of 2.19 mg KOH / g, a moisture and volatile matter content of 1.09%, a refractive index of 1.56, and an iodine value of 70.31 gI 2 / 100g, and the saponification value is 192.13 mg KOH / g.

[0055] In some preferred embodiments, the egg yolk oil has an acid value of 1.69 mg KOH / g, a hydrogen peroxide value of 1.42 mg KOH / g, a moisture and volatile matter content of 0.70%, a refractive index of 1.57, and an iodine value of 70.01 gI 2 / 100g, and the saponification value is 189.31 mg KOH / g.

[0056] In some more preferred embodiments, the egg yolk oil has an acid value of 0.13 mg KOH / g, a hydrogen peroxide value of 0.44 mg KOH / g, a moisture and volatile matter content of 0.45%, a refractive index of 1.50, and an iodine value of 69.19 gI 2 / 100g, saponification value is 189.36 mg KOH / g.

[0057] In some embodiments, the HPID can be obtained by conventional preparation methods in the art, for example, by esterification reaction of hydroxyl groups (OH groups) of isostearyl alcohol and phytosterol and carboxyl groups (COOH groups) of dimerized linoleic acid.

[0058] The second aspect of the present invention provides a skin care product, which includes the bionic fetal skin fat composition as described above and auxiliary materials.

[0059] In the present invention, the skin care products are not particularly limited, and generally can include one or more of face cream, essence milk, makeup remover cream and makeup remover oil.

[0060] In some embodiments, based on the total mass of the skin care product, the mass content of the macadamia nut oil is 0.01%-10%, preferably 0.03%-10%, for example 1% or 5%.

[0061] In some embodiments, based on the total mass of the skin care product, the mass content of the egg yolk oil is 0.01%-10%, preferably 0.03%-5%, for example 0.16% or 1%.

[0062] In some embodiments, based on the total mass of the skin care product, the mass content of the HPID is 0.01%-10%, preferably 0.03%-10%, for example 0.16%, 1% or 5%.

[0063] In some preferred embodiments, the skin care product comprises: 0.03-10% macadamia nut oil, 0.03-5% egg yolk oil and 0.03-10% HPID.

[0064] In some specific embodiments, the skin care product comprises: 0.03% macadamia oil, 0.03% egg yolk oil and 0.03% HPID.

[0065] In some specific embodiments, the skin care product comprises: 1% macadamia oil, 1% egg yolk oil and 5% HPID.

[0066] In some specific embodiments, the skin care product comprises: 1% macadamia oil, 1% egg yolk oil and 10% HPID.

[0067] In some specific embodiments, the skin care product comprises: 0.16% macadamia oil, 0.16% egg yolk oil and 0.16% HPID.

[0068] In some specific embodiments, the skin care product comprises: 5% macadamia oil, 1% egg yolk oil and 1% HPID.

[0069] In some specific embodiments, the skin care product comprises: 10% macadamia oil, 5% egg yolk oil and 1% HPID.

[0070] In the present invention, the auxiliary materials may be conventional auxiliary materials in the field of skin care products, and those skilled in the art may select them accordingly according to the specific product form of the skin care products.

[0071] In some embodiments, the skin care product is an essence lotion, and the auxiliary materials include one or more of a solvent, a preservative, a thickener, and an emulsifier.

[0072] The solvent may be conventional in the art, such as water.

[0073] Wherein, based on the total mass of the skin care product, the mass content of the solvent in the essence milk is preferably 80%-97%.

[0074] The preservative may be conventional in the art, such as p-hydroxyacetophenone and / or 1,2-hexanediol, preferably p-hydroxyacetophenone and 1,2-hexanediol.

[0075] Wherein, based on the total mass of the skin care product, the mass content of the preservative in the essence milk is preferably 1%.

[0076] Wherein, the thickener can be conventional in the art, such as xanthan gum.

[0077] Wherein, based on the total mass of the skin care product, the mass content of the thickener in the essence milk is preferably 0.2%.

[0078] The emulsifier may be conventional in the art, such as AVC (a mixture of ammonium acryloyldimethyltaurate and CP copolymer).

[0079] Wherein, based on the total mass of the skin care product, the mass content of the emulsifier in the essence milk is preferably 2%.

[0080] The third aspect of the present invention also provides a use of the bionic fetal skin fat composition as described above in the preparation of skin care products.

[0081] In some embodiments, the skin care product has moisturizing and / or skin barrier stabilizing effects.

[0082] On the basis of being in accordance with the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0083] The reagents and raw materials used in the present invention are commercially available.

[0084] The positive and progressive effects of the present invention are:

[0085] The bionic fetal skin fat composition of the present invention comprises three specific active ingredients (macadamia nut oil, egg yolk oil and HPID); among them, macadamia nut oil and egg yolk oil have extremely high sebum similarity, and HPID has extremely high intercellular lipid similarity; the skin care product prepared by the synergistic compounding of the three has excellent moisturizing performance and can also stabilize the skin barrier, and is particularly suitable for the skin of infants and children. BRIEF DESCRIPTION OF THE DRAWINGS

[0086] Figure 1 It is the water content of the stratum corneum of the skin 4 hours after applying the essence lotions of Examples 1-6 and Comparative Examples 1-11 in Effect Example 2 of the present invention.

[0087] Figure 2 It is the water content of the stratum corneum of the skin after applying the essence lotions of Examples 1-6 and Comparative Examples 1-11 for 8 hours in Effect Example 2 of the present invention.

[0088] Figure 3 The results of transepidermal water loss after applying the essence lotions of Examples 1-6 and Comparative Examples 1-11 for 4 hours in Effect Example 2 of the present invention are shown.

[0089] Figure 4The results of transepidermal water loss after 8 hours of using the essence lotions of Examples 1-6 and Comparative Examples 1-11 in Effect Example 2 of the present invention.

[0090] Figure 5 This is the improvement result of the skin barrier after applying the essence lotions of Examples 1-6 and Comparative Examples 1-11 in Effect Example 3 of the present invention. DETAILED DESCRIPTION

[0091] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. The experimental methods in the following examples without specifying specific conditions are carried out according to conventional methods and conditions, or selected according to the product specifications.

[0092] In the present invention, the preparation of each raw material is specifically as follows:

[0093] 1. Egg yolk oil

[0094] The following three methods were used for preparation:

[0095] (1) High temperature frying method: Bake the egg yolk at 285℃ for 45 minutes.

[0096] (2) Solvent method: Place 500 g of egg yolk and 1000 mL of ethanol in a four-necked flask, stir (120 r / min), place in a 75°C constant temperature water bath, and extract for 1 h.

[0097] (3) Supercritical method: egg yolk powder is subjected to CO 2 Supercritical extraction, extraction time is 5h, extraction temperature is 55℃.

[0098] 2. Macadamia Oil

[0099] The following three methods were used for preparation:

[0100] (1) Pressing method: Grind the macadamia nut oil into a uniform slurry, then press it (pressing speed is 30r / min, pressing temperature is room temperature), and filter the oil.

[0101] (2) Solvent method: Grind 500g of macadamia nut oil into a uniform slurry, add 1500mL of ethanol solvent, extract (50℃, 4h, set the speed to 500rpm), and finally remove the solvent using a rotary evaporator at 50℃, and filter the oil to obtain the product.

[0102] (3) Aqueous preparation method: Grind macadamia nut oil into a uniform slurry, add distilled water, extract under magnetic stirring (600 rpm) at room temperature for 4 hours, then freeze-thaw the extract overnight, thaw in a 50°C water bath, centrifuge at 4800 rpm for 30 minutes after thawing, and separate the upper oil phase. Repeat the extraction of the residue 2-3 times under the same conditions, and combine the oil phases to obtain the product.

[0103] 3. HPID (phytosteryl isostearyl dimer linoleate)

[0104] The preparation is carried out by esterification reaction of the hydroxyl group (OH group) of isostearyl alcohol and phytosterol and the carboxyl group (COOH group) of dimerized linoleic acid, specifically referring to the conditions of Example 2 of patent CN114533583A:

[0105] Hydrogenated dimer acid: phytosterol: behenyl alcohol: stearyl alcohol: cetyl alcohol: isostearyl alcohol was added in a molar ratio of 100:45:90:45:15:5. The actual amount of hydrogenated dimer acid 563g, phytosterol 186.6g, behenyl alcohol 294g, stearyl alcohol 121.7g, cetyl alcohol 36.4g, isostearyl alcohol 13.5g was added to a 3L flask, 1g p-toluenesulfonic acid was added, 500g n-octane was used as solvent, and 130°C was refluxed for 20 hours (water was discharged through a liquid separator). After the reaction was completed, n-octane was recovered under 90°C vacuum conditions, washed twice with 500g 50% propanol solution, and then 300g water vapor was introduced under vacuum conditions for deodorization, and finally 80°C was kept under vacuum for 2h to remove water to obtain 1182g of product.

[0106] Accordingly, the physicochemical properties of egg yolk oil and macadamia nut oil obtained by each preparation method were determined by the method recommended by AOAC (2000), as shown in Tables 1 and 2:

[0107] Table 1 Egg yolk oil yield and basic physical and chemical properties parameters

[0108]

[0109] Table 2 Macadamia nut oil yield and basic physical and chemical properties performance parameters

[0110]

[0111] As can be seen from Table 1, the acid value (AV) and peroxide value (POV) of egg yolk oil prepared by different processing methods were 0.13-3.65 mg KOH / g and 0.44-2.19 mg KOH / g, respectively, and there were differences between different processing methods.

[0112] As can be seen from Table 2, the acid value (AV) and peroxide value (POV) of macadamia oil prepared by different processing methods are 0.43-1.07 mg KOH / g and 0.2-0.44 mg KOH / g, respectively, and there are differences between different processing methods.

[0113] Based on the comprehensive oil yield and physicochemical indicators, egg yolk oil and macadamia oil were prepared by supercritical method and aqueous method respectively, and the bionic fetal skin fat composition and skin care products containing the same of each embodiment and comparative example were prepared according to the corresponding ratio.

[0114] Examples 1-6 and Comparative Examples 1-11

[0115] The raw materials were mixed evenly in the proportions shown in Table 3 to prepare essence milks containing different bionic fetal skin fat compositions:

[0116] Table 3 Formulas of essence lotions of Examples 1-6 and Comparative Examples 1-11

[0117]

[0118] Among them, p-hydroxyacetophenone was provided by Thor, 1,2-hexanediol was provided by Thor, xanthan gum was provided by DSM, and AVC was provided by Klein.

[0119] Effect Example 1 Correlation test between components of bionic fetal skin fat composition and components of infant skin fat:

[0120] The test is performed in the following steps:

[0121] (1) Volunteer screening: Volunteer database screening, subjects aged 0-6 years with transdermal water loss ≥15g / (cm²·h).

[0122] (2) Sebum membrane sampling: collect infant sebum, record the weight with sebum tape, and store in 5 mL EP tube at -80℃ for inspection.

[0123] (3) Sample processing: Cut the sample into pieces.

[0124] (4) Sample testing:

[0125] ① Take 100 μL of sample, add 400 μL of MTBE, vortex mix, and ultrasonicate in an ice-water bath to obtain a mixed solution.

[0126] ②Take 50 μL of the mixed solution, add 150 μL of water, and then add 480 μL of the extract (MTBE: MeOH = 10: 1, vortex to mix, sonicate in an ice water bath, centrifuge at 4 ℃, 3000 rpm for 15 min, and take 250 μL of the supernatant. Then add 250 μL of MTBE, vortex, sonicate, centrifuge at 4 ℃, 3000 rpm for 15 min, and take 250 μL of the supernatant. Then add 250 μL of MTBE, vortex, sonicate, and centrifuge at 4 ℃, 3000 rpm for 15 min, and take 250 μL of the supernatant.

[0127] ③ Combine the supernatants from the above three times and dry them under vacuum at 37 °C; add 100 μL of a solution (DCM: MeOH: H 2 O = 60: 30: 4.5) for reconstitution, vortex, sonicate in an ice-water bath, and centrifuge at 4 °C, 12000 rpm for 15 min; take 35 μL of the supernatant into a sample injection bottle for later use.

[0128] (5) On-machine testing

[0129] This project used a Nexera LC-40 ultra-high performance liquid chromatograph to perform chromatographic separation of the target compounds using liquid chromatography columns, and a SCIEX Triple Quad™ 7500 mass spectrometer for analysis and data acquisition.

[0130] ①Nexera LC-40 liquid phase instrument parameter settings:

[0131] Liquid chromatography phase A is 40% water and 60% acetonitrile solution, containing 10 mmol / L ammonium acetate; phase B is 10% acetonitrile and 90% isopropanol solution, containing 10 mmol / L ammonium acetate. Mobile phase flow rate: 0.3 mL / min, column temperature: 45 ℃, sample plate temperature: 10 ℃, injection volume: 2 μL.

[0132] ② During data acquisition, mass spectrometry analysis was performed in multiple reaction monitoring (MRM) mode. The ion source parameters were as follows: IonSpray Voltage: +3500 / -3000 V, Curtain Gas: 50 psi, Temperature: 400℃, IonSource Gas 1: 50 psi, Ion Source Gas 2: 50 psi.

[0133] ③ Use RT peak time of 2.5min-15min as quality control for sebum type detection.

[0134] (6) Data processing

[0135] All target compound analysis and quantification work was completed using BioBud (v2.0.3). The quantitative results of each lipid in the sample are shown in Table 4. The concentration of each lipid under test, Conc[Std] (μg / mL), was calculated based on the peak area and actual concentration relationship of the internal standard (IS) of the same lipid. The concentration of the lipid to be tested in the sample, Conc[Spl], is equal to the concentration of the lipid to be tested, Conc[Std], multiplied by the volume of the extract, Vol[Extractor] (mL), divided by the sample mass, Amount[Spl] (mg), in μg / mg.

[0136] The concentration of the lipid to be tested in the sample is calculated according to the following formula:

[0137]

[0138] Where Conc[Spl] is the concentration of the analyte in the sample (μg / mg); Conc[Std] is the concentration of the analyte at the extraction site (μg / mL); Vol[Extractor] is the volume of the extract (mL); and Amount[Spl] is the sample mass (mg).

[0139] (7) Test results

[0140] As shown in Table 4, measured by the method in this effect example, the baby sebum components include 19 categories, specifically:

[0141] ① Free fatty acids; ② Glycerides: monosaccharide diglyceride, disaccharide diglyceride, diglyceride, triglyceride, cholesterol ester, hemimonophosphoglyceride; ③ Ceramides: ceramide, dihydroceramide, hexosylceramide; ④ Phospholipids: sphingomyelin, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine.

[0142] Furthermore, for the egg yolk oil and macadamia oil used in each embodiment of the present invention, it can be seen from the results in Table 4 that, from the perspective of sebum detection components, the components of egg yolk oil and macadamia oil are basically similar to those of baby sebum, that is, egg yolk oil and macadamia oil have sebum similarity.

[0143] Table 4 Test results of baby sebum components and sebum-like components of egg yolk oil and macadamia nut oil

[0144]

[0145] Effect Example 2 Skin moisturizing effect test:

[0146] 1. Test conditions

[0147] (1) Eligible subjects: 10 volunteers aged between 18 and 35 years old, who have not used any medication that may affect skin pigment metabolism within 3 natural months. The skin test site must have no obvious pigmentation, no allergies, and no history of heavy sun exposure.

[0148] (2) Environmental conditions: The test should be carried out continuously in a constant temperature and humidity environment indoors. The subjects should sit and rest for at least 30 minutes in advance and the tested area should be evenly exposed within 10 minutes before the start of the measurement. Each test parameter must be measured repeatedly at least 3 times and the average value should be taken.

[0149] Specifically, 10 healthy Chinese subjects were selected, and the subjects used the test samples (i.e., the essence lotions of Examples 1-2 and Comparative Examples 1-11) for 4 h and 8 h, respectively. The water content of the stratum corneum of the dry parts of the subjects' calves and the transepidermal water loss were compared before and after use, and the moisturizing effect of the test samples was evaluated.

[0150] 2. Test results

[0151] (1) Moisture content (unit: au) test results

[0152] like Figure 1 As shown, after 4 hours of use, the moisture content of the blank group was 13.33, and the moisture content of the matrix milk group (refer to Table 3, without bionic fetal skin fat composition) was 19.00; after 4 hours of use of the essence milk of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, Example 6, the moisture contents were 21.33, 23.67, 25.67, 27.00, 25.00, 27.67, 41.33, 37.67, 43.33, 33.00, 31.33, 35.00, 34.33, 34.00, 46.00, 42.33, and 42.67, respectively.

[0153] like Figure 2 As shown, after 8 hours of use, the moisture content of the blank group was 13.33, and the moisture content of the matrix milk group (refer to Table 3, without bionic fetal skin fat composition) was 19.00; after 8 hours of use of the essence milk of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, and Example 6, the moisture contents were 14.67, 15.00, 17.33, 16.33, 17.33, 18.33, 27.00, 28.00, 28.33, 15.00, 24.67, 21.67, 24.33, 26.00, 36.33, 37.00, and 36.67, respectively.

[0154] (2) Transepidermal water loss (unit: g·h -1 ·m -2 ) Test Results

[0155] like Figure 3 As shown, after 4 hours of use, the transepidermal water loss of the blank group was 25.67, the transepidermal water loss of the matrix milk group (refer to Table 3, without the bionic fetal skin fat composition) was 26.00, and after 4 hours of use of the essence milk of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, and Example 6, the transepidermal water loss was 24.00, 23.00, 24.33, 22.67, 23.33, 23.33, 20.67, 20.00, 19.67, 18.33, 21.00, 22.00, 21.33, 19.67, 16.67, 17.00, and 17.33, respectively.

[0156] like Figure 4 As shown, after 8 hours of use, the transepidermal water loss content of the blank group was 23.33, the transepidermal water loss of the matrix milk group (refer to Table 3, without bionic fetal skin fat composition) was 23.67, and after 8 hours of use of the essence milk of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, and Example 6, the transepidermal water loss was 23.33, 23.33, 24.33, 22.67, 23.33, 23.33, 20.67, 20.33, 21.00, 21.00, 21.00, 21.00, 21.00, 20.00, 18.67, 19.00, and 18.67, respectively.

[0157] It can be seen that when the total content of the bionic fetal skin fat composition is consistent, especially at a low content (such as the mass content of the bionic fetal skin fat composition is 0.5%), the three components can achieve better effects than a single component or any two components.

[0158] Effect Example 3 Skin barrier protection effect test

[0159] 1. Test steps:

[0160] (1) 3D epidermal model culture:

[0161] The 3D epidermal skin model (Epskin) (Shanghai Si'anfuno Biotechnology Co., Ltd.) was removed from the agar medium, the adhered agar was wiped off with a sterile filter paper strip, and transferred to a 12-well plate with 2 mL of maintenance medium added to each well, and cultured for 24 h.

[0162] (2) Sample settings:

[0163] The 3D epidermal skin model obtained in step (1) was tested in the following groups, with 25 μL of culture medium in each well:

[0164] BC group: blank group, containing only 3D epidermal skin model, without test substance application and SDS induction;

[0165] NC group: positive group, containing 3D epidermal skin model, induced only with SDS, without application of test substance;

[0166] Sample group: 12 groups were set up in total, including 3D epidermal skin models, all of which were induced with SDS and smeared with the essence lotions of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, and Example 6, respectively, with 25 μL of the test substance applied to each group.

[0167] After culturing for 24 hours, the obtained 3D epidermal skin model was subjected to HE staining.

[0168] (3) Testing indicators:

[0169] The 3D epidermal skin model was fixed with 4% paraformaldehyde for 48 hours, then dehydrated with gradient ethanol, transparentized with xylene, embedded in paraffin, and sliced ​​after freezing with a slice thickness of 3 μm. The following operations were performed in turn for the following test indicators:

[0170] ① Hematoxylin staining of cell nuclei: Stain the sections with hematoxylin for 3-8 minutes and rinse with tap water; differentiate with 1% hydrochloric acid alcohol for a few seconds and rinse with tap water; turn blue with 0.6% ammonia water and rinse with running water.

[0171] ②Eosin staining of cytoplasm: Slice into eosin staining solution and stain for 1-3 minutes.

[0172] Finally, dehydrate and seal the sections: place the sections in 95% alcohol I for 5 min, 95% alcohol II for 5 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, xylene I for 5 min, and xylene II for 5 min to dehydrate and make them transparent. Take the sections out of xylene and dry them slightly, seal them with neutral gum, and take pictures under a microscope.

[0173] 2. Test results

[0174] like Figure 5 As shown, through the 3D epidermal skin model, compared with the blank group, the stratum corneum skin barrier was significantly damaged after applying the SDS group (i.e., the NC group), and the stratum corneum was loosely connected, while the 3D epidermal skin model samples to be tested after adding the essence lotions of Comparative Examples 1-6, Example 1, Example 2, Example 3, Comparative Examples 7-11 and Example 4, Example 5, and Example 6 achieved different degrees of increase in stratum corneum integrity and tissue vitality.

[0175] Specifically, the essence emulsion containing a single bionic fetal skin fat composition component can increase the integrity of the stratum corneum and maintain tissue vitality in Examples 1-3, and the effect is better than that in Comparative Examples 1-6; Examples 4-6 can increase the integrity of the stratum corneum and maintain tissue vitality, and the effect is better than that in Comparative Examples 7-11. Furthermore, when the total content of the bionic fetal skin fat composition is the same, the effect of the three components is significantly better, indicating that there is a significant synergistic effect when the three components are compounded.

Claims

1. A bionic fetal skin fat composition, characterized in that: The bionic fetal skin fat composition comprises the following components in parts by mass: 0.01-10 parts macadamia oil, 0.01-10 parts egg yolk oil and 0.01-10 parts HPID.

2. The bionic fetal skin fat composition according to claim 1, characterized in that In the bionic fetal skin fat composition, the mass fraction of the macadamia nut oil is 0.03-10 parts, for example, 1 or 5 parts; And / or, in the bionic fetal skin fat composition, the egg yolk oil has a mass fraction of 0.03-5 parts, such as 0.16 parts or 1 part; And / or, in the bionic fetal skin fat composition, the mass fraction of the HPID is 0.03-10 parts, such as 0.16 parts, 1 parts or 5 parts; Preferably, the bionic fetal skin fat composition comprises: 0.03-10 parts of macadamia nut oil, 0.03-5 parts of egg yolk oil and 0.03-10 parts of HPID; More preferably, the bionic fetal skin fat composition comprises: 0.03 parts of macadamia nut oil, 0.03 parts of egg yolk oil and 0.03 parts of HPID; More preferably, the bionic fetal skin fat composition comprises: 1 part of macadamia nut oil, 1 part of egg yolk oil and 5 parts of HPID; More preferably, the bionic fetal skin fat composition comprises: 1 part of macadamia nut oil, 1 part of egg yolk oil and 10 parts of HPID; More preferably, the bionic fetal skin fat composition comprises: 0.16 parts of macadamia nut oil, 0.16 parts of egg yolk oil and 0.16 parts of HPID; More preferably, the bionic fetal skin fat composition comprises: 5 parts of macadamia nut oil, 1 part of egg yolk oil and 1 part of HPID; More preferably, the bionic fetal skin fat composition comprises: 10 parts of macadamia nut oil, 5 parts of egg yolk oil and 1 part of HPID.

3. The bionic fetal skin fat composition according to claim 1, characterized in that The macadamia nut oil comprises: one or more of free fatty acids, glyceride compounds, ceramide compounds and phospholipid compounds; Wherein, the glyceride compound preferably includes one or more of monosaccharide diglyceride, disaccharide diglyceride, diglyceride, triglyceride, cholesterol ester and hemimonophosphate glyceride; Wherein, the ceramide compound preferably includes one or more of ceramide, dihydroceramide and hexosylceramide; Wherein, the phospholipid compound preferably includes one or more of sphingomyelin, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine; Preferably, the macadamia nut oil comprises one or more of free fatty acids, monosaccharide diglycerides, disaccharide diglycerides, diglycerides, triglycerides, cholesterol esters, hemimonophosphoglycerides, ceramides, dihydroceramides, hexosylceramides, sphingomyelins, lysophosphatidylethanolamines, lysophosphatidylglycerols, lysophosphatidylserines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines; More preferably, the macadamia nut oil comprises the following components by mass percentage: 0-8% free fatty acids, 0-5% monosaccharide diglycerides, 0-5% disaccharide diglycerides, 0-5% diglycerides, 90-100% triglycerides, 0-5% cholesterol esters, 0-5% ceramides, 0-5% dihydroceramides, 0-5% hexosylceramides, 0-5% sphingomyelins, 0-5% lysophosphatidylethanolamines, 0-5% lysophosphatidylglycerols, 0-5% lysophosphatidylserines, 0-5% phosphatidylcholine, 0-5% phosphatidylethanolamines, 0-5% phosphatidylglycerols, 0-5% phosphatidylinositols, 0-5% phosphatidylserines and 0-5% hemimonophosphoglycerides; And / or, the macadamia nut oil is produced by a pressing method, a solvent method or an aqueous method, preferably by an aqueous method.

4. The bionic fetal skin fat composition according to claim 1, characterized in that The macadamia nut oil has an acid value of 0.40-1.10 mg KOH / g, such as 0.43 mg KOH / g, 0.68 mg KOH / g or 1.07 mg KOH / g; and / or, the macadamia nut oil has a hydrogen peroxide value of 0.10-0.50 mg KOH / g, such as 0.2 mg KOH / g, 0.32 mg KOH / g or 0.44 mg KOH / g; and / or, the water and volatile matter content of the macadamia nut oil is 0.01-0.70%, such as 0.45% or 0.62%; And / or, the refractive index of the macadamia oil is 1.40-1.60, such as 1.50, 1.56 or 1.57; and / or, the iodine value of the macadamia nut oil is 68.00-72.00 gI2 / 100g, such as 69.19 gI2 / 100g, 70.21 gI2 / 100g or 71.31 gI2 / 100g; and / or, the macadamia nut oil has a saponification value of 185.00-192.00 mg KOH / g, such as 189.01 mg KOH / g, 189.36 mg KOH / g or 190.13 mg KOH / g; Preferably, the macadamia nut oil has an acid value of 1.07 mg KOH / g, a hydrogen peroxide value of 0.2 mg KOH / g, a moisture and volatile matter content of 0.01%, a refractive index of 1.56, an iodine value of 71.31 gI2 / 100g, and a saponification value of 190.13 mgKOH / g; Preferably, the macadamia nut oil has an acid value of 0.68 mg KOH / g, a hydrogen peroxide value of 0.32 mg KOH / g, a moisture and volatile matter content of 0.62%, a refractive index of 1.57, an iodine value of 70.21 gI2 / 100g, and a saponification value of 189.01 mgKOH / g; More preferably, the macadamia nut oil has an acid value of 0.43 mg KOH / g, a hydrogen peroxide value of 0.44 mg KOH / g, a moisture and volatile matter content of 0.45%, a refractive index of 1.50, an iodine value of 69.19 gI2 / 100g, and a saponification value of 189.36 mgKOH / g.

5. The bionic fetal skin fat composition according to claim 1, characterized in that The egg yolk oil comprises: one or more of free fatty acids, glyceride compounds, ceramide compounds and phospholipid compounds; Wherein, the glyceride compound preferably includes one or more of monosaccharide diglyceride, disaccharide diglyceride, diglyceride, triglyceride and cholesterol ester; Wherein, the ceramide compound preferably includes one or more of ceramide, dihydroceramide and hexosylceramide; Wherein, the phospholipid compound preferably includes one or more of sphingomyelin, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine; Preferably, the egg yolk oil comprises one or more of free fatty acids, monosaccharide diglycerides, disaccharide diglycerides, diglycerides, triglycerides, cholesterol esters, ceramides, dihydroceramides, hexosylceramides, sphingomyelins, lysophosphatidylethanolamines, lysophosphatidylglycerols, lysophosphatidylserines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines; Preferably, the egg yolk oil comprises the following components by mass percentage: 0-7% free fatty acids, 0-5% monosaccharide diglycerides, 0-5% disaccharide diglycerides, 34-54% diglycerides, 46-56% triglycerides, 3-13% cholesterol esters, 0-5% ceramides, 0-5% dihydroceramides, 0-5% hexosylceramides, 0-5% lysophosphatidylethanolamines, 0-5% lysophosphatidylserines, 0-5% phosphatidylcholine, 0-5% phosphatidylethanolamines, 0-5% phosphatidylglycerols, 0-5% phosphatidylinositols and 0-5% phosphatidylserines; And / or, the egg yolk oil is prepared by high temperature frying method, solvent method or supercritical method; preferably, it is prepared by supercritical method.

6. The bionic fetal skin fat composition according to claim 1, characterized in that The acid value of the egg yolk oil is 0.10-3.70 mg KOH / g, such as 0.13 mg KOH / g, 1.69 mg KOH / g or 3.65 mg KOH / g; and / or, the egg yolk oil has a hydrogen peroxide value of 0.40-2.20 mg KOH / g, such as 0.44 mg KOH / g, 1.42 mg KOH / g or 2.19 mg KOH / g; and / or, the egg yolk oil has a moisture and volatile matter content of 0.40-1.10%, such as 0.45%, 0.70% or 1.09%; and / or, the refractive index of the egg yolk oil is 1.40-1.60, such as 1.50, 1.56 or 1.57; and / or, the iodine value of the egg yolk oil is 69.00-71.00 gI2 / 100g, such as 69.19 gI2 / 100g, 70.01 gI2 / 100g or 70.31 gI2 / 100g; and / or, the egg yolk oil has a saponification value of 188.00-193.00 mg KOH / g, such as 189.31 mg KOH / g, 189.36 mg KOH / g or 192.13 mg KOH / g; Preferably, the egg yolk oil has an acid value of 3.65 mg KOH / g, a hydrogen peroxide value of 2.19 mg KOH / g, a moisture and volatile matter content of 1.09%, a refractive index of 1.56, an iodine value of 70.31 gI2 / 100g, and a saponification value of 192.13 mg KOH / g; Preferably, the egg yolk oil has an acid value of 1.69 mg KOH / g, a hydrogen peroxide value of 1.42 mg KOH / g, a moisture and volatile matter content of 0.70%, a refractive index of 1.57, an iodine value of 70.01 gI2 / 100g, and a saponification value of 189.31 mg KOH / g; More preferably, the egg yolk oil has an acid value of 0.13 mg KOH / g, a hydrogen peroxide value of 0.44 mg KOH / g, a moisture and volatile matter content of 0.45%, a refractive index of 1.50, an iodine value of 69.19 gI2 / 100g, and a saponification value of 189.36 mg KOH / g.

7. A skin care product, characterized in that: The skin care product comprises the bionic fetal skin fat composition as described in any one of claims 1 to 6 and auxiliary materials.

8. The skin care product according to claim 7, characterized in that The skin care products include one or more of face cream, essence milk, makeup remover cream and makeup remover oil; And / or, based on the total mass of the skin care product, the mass content of the macadamia nut oil is 0.01%-10%, preferably 0.03%-10%, for example 1% or 5%; And / or, based on the total mass of the skin care product, the mass content of the egg yolk oil is 0.01%-10%, preferably 0.03%-5%, for example 0.16% or 1%; And / or, based on the total mass of the skin care product, the mass content of the HPID is 0.01%-10%, preferably 0.03%-10%, for example 0.16%, 1% or 5%; Preferably, the skin care product comprises: 0.03-10% macadamia nut oil, 0.03-5% egg yolk oil and 0.03-10% HPID; More preferably, the skin care product comprises: 0.03% macadamia nut oil, 0.03% egg yolk oil and 0.03% HPID; More preferably, the skin care product comprises: 1% macadamia nut oil, 1% egg yolk oil and 5% HPID; More preferably, the skin care product comprises: 1% macadamia nut oil, 1% egg yolk oil and 10% HPID; More preferably, the skin care product comprises: 0.16% macadamia nut oil, 0.16% egg yolk oil and 0.16% HPID; More preferably, the skin care product comprises: 5% macadamia nut oil, 1% egg yolk oil and 1% HPID; More preferably, the skin care product comprises: 10% macadamia nut oil, 5% egg yolk oil and 1% HPID.

9. Use of the bionic fetal skin fat composition according to any one of claims 1 to 6 in the preparation of skin care products.

10. The use according to claim 9, characterized in that The skin care product has moisturizing and / or skin barrier stabilizing effects.

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