A precise sampling device for detection of tumor cell pleuroperitoneal metastasis

Abstract

The application provides a sampling device for tumor cell thoracoabdominal membrane metastasis detection, which comprises a liquid storage container, a sampling assembly, a sampling assembly and an electromagnetic stirring assembly. The electromagnetic stirring assembly comprises an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container and a magnetic core arranged on the inner wall of the bottom of the liquid storage container. When the electromagnetic stirring assembly works, the magnetic field force generated thereby can drive the sample liquid in the liquid storage container to be uniformly stirred. The sampling assembly and the sampling assembly are each provided with a control system (i.e. a flow controller and an electromagnetic valve). When the preset capacity is reached, the flow controller can control the electromagnetic valve to be automatically closed. The sample liquid is fully and uniformly stirred by the electromagnetic stirrer, and is accurately divided into multiple sampling tubes, so that the accuracy of the tumor cell thoracoabdominal membrane metastasis detection result and the processing efficiency are improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medical devices, and particularly relates to a sampling device for detecting tumor cell thoraco-peritoneal metastasis. BACKGROUND

[0002] Tumor cell peritoneal metastasis is a common tumor metastasis route at present, especially in abdominal organ malignant tumors such as gastric cancer, colorectal cancer, etc. The incidence of tumor cell peritoneal metastasis is relatively high, which seriously affects the survival prognosis of patients. Most of the tumor cell peritoneal metastasis is through direct spread and lymphatic metastasis. Early tumor cell peritoneal metastasis is difficult to be screened out through imaging detection and ctDNA (circulating tumor DNA) blood detection.

[0003] At present, cytological examination of peritoneal lavage fluid can be used as the gold standard for diagnosing free tumor cells in the abdominal cavity. The main operation is to flush the abdominal cavity and peritoneum through lavage fluid. Tumor cells existing in the abdominal cavity will fall off into the lavage fluid under the flushing of the lavage fluid, and then the lavage fluid is extracted. The thoraco-abdominal lavage fluid is used as a detection sample for cytological examination or ctDNA (circulating tumor DNA) detection based on the lavage fluid. In order to screen tumor cell metastasis in early stage and take relevant medical measures in time.

[0004] For the obtained thoraco-abdominal lavage fluid, there is no mature thoraco-peritoneal tumor cell collection device at present. The lavage fluid is mainly introduced into a storage device by manual operation for storage, or introduced into a suction ball by pressing a suction ball to form negative pressure for storage, and then manually divided into multiple sampling tubes for tumor cell metastasis detection. However, in actual application, it is found that many factors will affect the accuracy of tumor cell thoraco-peritoneal metastasis detection results, mainly including: 1) the storage container is placed for too long time, which will cause cell precipitation in the lavage fluid, uneven distribution of tumor cells in the sample liquid, and thus affect the content of tumor cells in the final sampling, resulting in deviation of the detection result; 2) human error, different operation time, operation method and operation force will cause inconsistent sample amount, and excessive or insufficient situation. These factors will reduce the detection accuracy, cause the possibility of false negative rate of the detection result, delay the valuable treatment time of the patient. At the same time, the inconsistent amount of tumor cells in different sampling tubes will cause inconsistent detection results, affect the reliability of the detection result, and cause diagnostic doubts. SUMMARY

[0005] The present application aims at the problems in the prior art, and provides a sampling device for detecting tumor cell thoraco-peritoneal metastasis, so as to improve the detection result accuracy and reliability.

[0006] In order to achieve the above technical purpose, the present application adopts the following technical scheme:

[0007] A sampling device for detecting tumor cell pleuroperitoneal metastasis, comprising: a liquid storage container, a sampling assembly, a sampling assembly, an electromagnetic stirring assembly, a negative pressure suction device, a sample suction device;

[0008] The liquid storage container is used for storing sample liquid, and is provided with a liquid storage container liquid inlet, a liquid storage container negative pressure suction port at the upper portion, and a liquid storage container liquid outlet at the bottom portion.

[0009] The sampling assembly comprises a sampling liquid inlet and a first connecting pipe, one end of the first connecting pipe is connected with the sampling liquid inlet, and the other end is connected with the liquid inlet of the liquid storage container.

[0010] The sampling assembly comprises a sampling liquid inlet and a first connecting pipe, one end of the first connecting pipe is connected with the sampling liquid inlet, and the other end is connected with the liquid inlet of the liquid storage container.

[0011] The electromagnetic stirring assembly comprises an electromagnetic stirrer arranged on the outer wall of the bottom portion of the liquid storage container and a magnetic core arranged on the inner wall of the bottom portion of the liquid storage container.

[0012] The sampling assembly and the sampling assembly are respectively provided with a sampling control switch and a sampling control switch.

[0013] Preferably, a first flow controller and a first electromagnetic valve are arranged on the first connecting pipe of the sampling assembly in sequence according to the liquid flow direction.

[0014] The first flow controller is provided with a control system, the input end of the control system is connected with the signal output end of the first flow controller, and the output end of the control system is connected with the first electromagnetic valve.

[0015] Preferably, the first flow controller and the first electromagnetic valve are connected with a power supply device.

[0016] Preferably, the first flow controller and the first electromagnetic valve are connected with a power supply device.

[0017] Preferably, a second flow controller and a second electromagnetic valve are arranged on the second connecting pipe of the sampling assembly in sequence according to the liquid flow direction.

[0018] The second flow controller is provided with a control system, the input end of the control system is connected with the signal output end of the second flow controller, and the output end of the control system is connected with the second electromagnetic valve.

[0019] Preferably, the second flow controller and the second electromagnetic valve are connected with a power supply device.

[0020] Preferably, the second flow controller and the second electromagnetic valve are connected with a power supply device.

[0021] Preferably, the electromagnetic stirring assembly is further provided with a stirring control switch, and the electromagnetic stirrer is connected with a power supply device.

[0022] Preferably, the liquid storage container is made of any one of glass and medical polymer materials.

[0023] Preferably, the first connecting pipe and the second connecting pipe are made of medical polymer materials.

[0024] Compared with the prior art, the present application has the following beneficial effects:

[0025] 1) By introducing the electromagnetic stirring technology, the rapid and thorough mixing of various substances in the peritoneal lavage fluid in the liquid storage container is realized, the uniformity of the metastatic cells possibly existing in the ascites is ensured, and the uniformity and consistency of the sample are ensured. Compared with the traditional stirring method, the electromagnetic stirring force is uniform and moderate, and the integrity of the cells can be better protected, the cell rupture and damage caused by excessive or uneven stirring during the sample processing process are reduced, and the risk of deviation of the tumor cell and ctDNA detection is caused.

[0026] 2) By using the two groups of control components (flow controllers and electromagnetic valves) for sampling and sampling, the sampling amount of the sample into the liquid storage container and the sampling amount of the sample into the sampling test tube are accurately controlled, the influence of the sampling amount on the detection result is avoided, and the consistency of the sampling amount is finally ensured.

[0027] 3) The automatic control is used to replace the manual operation, the storage and stirring process of the lavage fluid are automatically controlled, the processing efficiency of the sample is greatly improved, the sampling, storage and sampling operation process of the lavage fluid are standardized, the operation time and human error in the detection are minimized, and a reliable, efficient and standardized sampling device is provided.

[0028] In summary, the present application can accurately divide and pack the lavage fluid aspirated from the abdominal cavity into a plurality of sampling tubes after sufficient and uniform stirring, avoid the influence of sample precipitation and human operation on the detection accuracy, ensure the uniformity and consistency of the sample, and improve the accuracy and reliability of the detection of the abdominal cavity metastasis of tumor cells. Meanwhile, the device is simple to operate, the process is standardized, and the sample processing efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 A longitudinal sectional view of an embodiment of the present application Figure 1 ;

[0030] Figure 2 A longitudinal sectional view of an embodiment of the present application Figure 2 ;

[0031] Figure 3 A transverse sectional view of A in the present application Figure 2 ;

[0032] Figure 4 for Figure 2 schematic view of a transverse section at B;

[0033] Figure 5 for Figure 2 schematic view of a transverse section at C;

[0034] Figure 6 for Figure 2 schematic view of a transverse section at D;

[0035] In the drawings: 1, sampling assembly; 2, liquid storage container; 3, electromagnetic stirring assembly; 4, sampling assembly; 5, electromagnetic stirrer; 6, magnetic core; 7, liquid inlet of liquid storage container; 8, negative pressure suction port of liquid storage container; 9, liquid outlet of liquid storage container; 10, sampling liquid inlet; 11, first flow controller; 12, first electromagnetic valve 1; 13, first connecting pipe; 14, second flow controller; 15, second electromagnetic valve; 16, second connecting pipe; 17, sampling liquid outlet; 18, stirring control switch; 19, sampling control switch; 20, sampling control switch. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present application will be described below with reference to the accompanying drawings of the embodiments of the present application. Figures 1-6 It should be clear that the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0037] In the description of the present application, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inner", "front", "central", "both ends" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the present application and simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. Figures 1-5 The orientation or positional relationship shown is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0038] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "arrangement", "connection", "fixing", "screw connection" and the like should be understood in a broad sense, for example, can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship of two elements, unless otherwise explicitly limited, the above-mentioned terms in the present application can be understood according to the specific meaning of the specific circumstances for the ordinary skilled in the art.

[0039] Embodiment 1

[0040] The present application provides a sampling device for detecting tumor cell pleuroperitoneal metastasis, which comprises a liquid storage container, a sampling assembly, a sampling assembly, an electromagnetic stirring assembly, a negative pressure suction device, a sample suction device;

[0041] The liquid storage container is used for storing sample liquid, and the upper part is provided with a liquid storage container liquid inlet and a liquid storage container negative pressure suction port, and the bottom is provided with a liquid storage container liquid outlet, and the negative pressure suction device is connected to the liquid storage container negative pressure suction port;

[0042] The sampling assembly comprises a sampling liquid inlet and a first connecting pipe, one end of the first connecting pipe is connected to the sampling liquid inlet, and the other end is connected to the liquid inlet of the liquid storage container, and the sample suction device is connected to the sampling liquid inlet;

[0043] The sampling assembly comprises a sampling liquid inlet and a first connecting pipe, one end of the first connecting pipe is connected to the sampling liquid inlet, and the other end is connected to the liquid inlet of the liquid storage container, and the sample suction device is connected to the sampling liquid inlet;

[0044] The electromagnetic stirring assembly comprises an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container and a magnetic core arranged on the inner wall of the bottom of the liquid storage container;

[0045] The sampling assembly and the sampling assembly are respectively provided with a sampling control switch and a sampling control switch. The valve type, medical clamp type control sampling and sampling start and stop can be used.

[0046] During sampling, the sample liquid drawn by the sample suction device enters from the sampling liquid inlet, flows through the first connecting pipe into the liquid inlet of the liquid storage container, and is stored in the liquid storage container; during sampling, the sample liquid flows through the second connecting pipe from the sampling liquid outlet, and finally flows into the external sampling test tube. During stirring, the electromagnetic stirring assembly works to generate a magnetic field force to drive the liquid in the liquid storage container to be uniformly stirred.

[0047] Embodiment 2

[0048] As Figures 1-6As shown, the embodiment of the present application provides a sampling device for detecting tumor cell pleuroperitoneal metastasis. The device needs to be connected with an external negative pressure suction device and a sample liquid suction device for use. The sampling device as a whole comprises a liquid storage container 2, a sampling assembly 1, a sampling assembly 4, an electromagnetic stirring assembly 3, a negative pressure suction device, and a sample liquid suction device.

[0049] The liquid storage container 2 in the middle position is used for storing sample liquid, and is provided with a liquid storage container liquid inlet 7 and a liquid storage container negative pressure suction port 8 at the upper portion and a liquid storage container liquid outlet 9 at the bottom. The negative pressure suction device is connected to the liquid storage container negative pressure suction port 8.

[0050] The sampling assembly 1 above comprises a sampling liquid inlet 10 and a first connecting pipe 13. One end of the first connecting pipe 13 is connected to the sampling liquid inlet 10, and the other end is connected to the liquid storage container liquid inlet 7. The sample liquid suction device is connected to the sampling liquid inlet 10.

[0051] The sampling assembly 4 below comprises a sampling liquid outlet 17 and a second connecting pipe 16. One end of the second connecting pipe 16 is connected to the liquid storage container liquid outlet 9, and the other end is connected to the sampling liquid outlet 17.

[0052] The electromagnetic stirring assembly 3 in the middle comprises an electromagnetic stirrer 5 arranged on the outer wall of the bottom of the liquid storage container and a magnetic core 6 arranged on the inner wall of the bottom of the liquid storage container.

[0053] The sampling assembly and the sampling assembly are respectively provided with a sampling control switch and a sampling control switch.

[0054] A first flow controller 11 and a first electromagnetic valve 12 are arranged in sequence on the first connecting pipe 13 of the sampling assembly 1 in the liquid flow direction.

[0055] The first flow controller 11 is provided with a control system. The input end of the control system is connected to the first flow controller 11, and the output end of the control system is connected to the first electromagnetic valve 12.

[0056] When the liquid storage container 2 is injected with sample liquid to reach a preset capacity, the first flow controller 11 will control the first electromagnetic valve 12 to close, stopping the injection of sample liquid.

[0057] A second flow controller 14 and a second electromagnetic valve 15 are arranged in sequence on the second connecting pipe 16 of the sampling assembly 4 in the liquid flow direction.

[0058] The second flow controller 14 is provided with a control system. The input end of the control system is connected to the second flow controller 14, and the output end of the control system is connected to the second electromagnetic valve 15.

[0059] When the external sampling test tube is injected with sample liquid to reach a preset capacity, the second flow controller 14 will control the second electromagnetic valve 15 to close, stopping the injection of sample liquid.

[0060] The electromagnetic stirring assembly 3 is further provided with a stirring control switch 18, which can manually start or stop the electromagnetic stirrer 5.

[0061] The magnetic core 6 can be in different shapes, including disc, bar, cross, etc.

[0062] The liquid storage container 2 can be made of different materials, preferably good magnetic field penetration materials, which can be any of glass and medical polymer materials.

[0063] The sampling assembly 1 is provided with a sampling control switch 20, which in this embodiment specifically controls the opening or closing of the first flow controller 11. The first flow controller 11 synchronously controls the opening and closing of the first electromagnetic valve 12, thereby controlling the start or stop of sampling.

[0064] The sampling assembly 4 is provided with a sampling control switch 19, which in this embodiment specifically controls the opening or closing of the second flow controller 14. The second flow controller 14 synchronously controls the opening and closing of the second electromagnetic valve 15, thereby controlling the start or stop of sampling.

[0065] The electromagnetic stirrer 5, the first flow controller 11, the first electromagnetic valve 12, the second flow controller 14, and the second electromagnetic valve 15 are connected with a power supply device. The power supply device can be a battery pack or an external power source.

[0066] The first flow controller 11 and the first connecting pipe 13, the first electromagnetic valve 12 and the first connecting pipe 13, the second flow controller 14 and the second connecting pipe 16, and the second electromagnetic valve 15 and the second connecting pipe 16 are all sealedly connected.

[0067] The first connecting pipe 13 and the second connecting pipe 16 are made of medical polymer materials.

[0068] The sampling device can be connected to various types of negative pressure suction devices, preferably a central negative pressure suction system, which is convenient to use and can provide stable suction force.

[0069] The sampling device can be connected to various types of sample liquid suction devices, which can include various flushing functions in addition to the sample liquid suction function.

[0070] The maximum capacity of the liquid storage container 2 is 500ml, and the pre-set capacity of the liquid storage container 2 is generally 350ml.

[0071] The pre-set capacity of the sampling test tube is generally 50ml, and generally 5 tubes need to be collected.

[0072] The use process of the sampling device for detecting tumor cell pleuroperitoneal metastasis is as follows:

[0073] 1) Preparation operation: the negative pressure suction device is connected to the negative pressure suction port 8 of the liquid storage container. One end of the sample suction device is connected to the sampling assembly 1 device, and the other end is fully lavaged in the patient's chest and abdominal cavity.

[0074] 2) Sampling process: open the sampling control switch 20. When the negative pressure suction device draws air in the liquid storage container 2 through the vacuum pump, negative pressure is generated in the closed liquid storage container 2, which promotes the sample liquid to flow from the sample suction device through the sampling assembly 1, and finally into the liquid storage container 2 from the liquid inlet 7 of the liquid storage container. When the capacity of the liquid storage container 2 reaches the preset capacity, the first flow controller 11 controls the first electromagnetic valve 12 to automatically close, and the sample liquid is no longer injected into the liquid storage container 2; at the same time, the negative pressure suction device and the sample suction device are closed. At this time, the sampling control switch 20 will be automatically closed.

[0075] 3) Stirring process: after 30-60 seconds of stagnation, open the stirring control switch 18. Because the magnetic core 6 can be subjected to a relatively uniform magnetic field force at each position in the liquid storage container 2, it performs regular circular motion, ensuring that the liquid is uniformly stirred throughout the container. Generally, after stirring for 15-30 seconds, the electric stirring control switch 18 is closed.

[0076] 4) Sampling process: at the same time, open the sampling control switch 19. The sample liquid flows from the liquid outlet 9 at the bottom of the liquid storage container 2 through the sampling assembly 4, and finally into the sampling test tube from the sampling outlet 17. When the sampling test tube reaches the preset capacity, the second flow controller 14 controls the second electromagnetic valve 15 to automatically close, and the sample liquid is no longer injected into the sampling test tube. At this time, the sampling control switch 19 will be automatically closed.

[0077] 5) Subsequent multiple repeated sampling operations will obtain multiple tubes of sample liquid. In addition, because a small amount of sample liquid remains in the connecting pipe and cannot be fully stirred, in order to ensure the uniformity and consistency of the sample, the selected sample for submission will start from the second tube.

[0078] The collection device of this embodiment can accurately divide the lavage fluid sucked from the abdominal cavity into multiple sampling tubes after sufficient and uniform stirring, avoiding the influence of sample liquid precipitation and human operation on the detection accuracy, ensuring the uniformity and consistency of the sample, and improving the accuracy of the detection of tumor cell abdominal metastasis. At the same time, the storage and access of the sample liquid are automatically controlled by the control components (flow controllers and electromagnetic valves), which is simple to operate and improves the sample processing efficiency.

[0079] The above only describes the embodiments of the present application and does not limit the present application. Any modification, equivalent replacement and improvement made within the scope of the application should be included in the protection scope of the present application.

Claims

1. A sampling device for detection of tumor cell pleuroperitoneal metastasis, characterized by: The sampling device comprises a liquid storage container, a sampling assembly, a sampling assembly, an electromagnetic stirring assembly, a negative pressure suction device, and a sample suction device. The liquid storage container is used for storing sample liquid, and is provided with a liquid inlet, a negative pressure suction port, and a liquid outlet. The sampling assembly comprises a sampling inlet and a first connecting pipe. The sampling assembly comprises a sampling outlet and a second connecting pipe. The electromagnetic stirring assembly comprises an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container and a magnetic core arranged on the inner wall of the bottom of the liquid storage container. The sampling assembly and the sampling assembly are respectively provided with a sampling control switch and a sampling control switch. The use process of the sampling device is as follows: 1) Preparation operation: the negative pressure suction device is connected to the negative pressure suction port of the liquid storage container, one end of the sample suction device is connected to the sampling assembly, and the other end is used for fully lavaging the chest and abdominal cavity of the patient; 2) Sampling process: open the sampling control switch, when the negative pressure suction device extracts air in the liquid storage container, negative pressure is generated in the closed liquid storage container, which promotes the sample liquid to flow through the sampling assembly from the sample suction device and finally injects into the liquid storage container through the liquid inlet of the liquid storage container; When the liquid storage container reaches the preset capacity, stop injecting sample liquid; at the same time, the negative pressure suction device and the sample suction device are closed; 3) Stirring process: after 30-60 seconds of stagnation, the electromagnetic stirring assembly is started to stir, and the stirring process lasts for 15 to 30 seconds, and the stirring is completed; 4) Sampling; 5) Subsequent multiple repeated sampling operations, and multiple tubes of sample liquid are obtained; in addition, a small amount of sample liquid is stored in the connecting pipe and cannot be fully stirred, in order to ensure the uniformity and consistency of the sample, the selected sample for inspection starts from the second tube.

2. The sampling device for detecting tumor cell pleuroperitoneal metastasis according to claim 1, characterized in that: A first flow controller and a first electromagnetic valve are arranged on the first connecting pipe of the sampling assembly in sequence according to the liquid flow direction. The first flow controller is provided with a control system, the input end of the control system is connected to the signal output end of the first flow controller, and the output end of the control system is connected to the first electromagnetic valve.

3. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 2, characterized in that: The first flow controller and the first electromagnetic valve are connected to a power supply device.

4. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 2, characterized in that: The first flow controller and the first electromagnetic valve are connected to the first connecting pipe in a sealed manner.

5. The sampling device for detecting tumor cell pleuroperitoneal metastasis according to claim 1, characterized in that: A second flow controller and a second electromagnetic valve are arranged on the second connecting pipe of the sampling assembly in sequence according to the liquid flow direction. The second flow controller is provided with a control system, an input end of the control system is connected with a signal output end of the second flow controller, and an output end of the control system is connected with the second electromagnetic valve.

6. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 5, characterized in that: The second flow controller and the second electromagnetic valve are connected with a power supply device.

7. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 5, characterized in that: The second flow controller and the second connecting pipe, and the second electromagnetic valve and the second connecting pipe are in sealed connection.

8. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 1, characterized in that: The electromagnetic stirring assembly is further provided with a stirring control switch, and the electromagnetic stirrer is connected with a power supply device.

9. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 1, characterized in that: The material of the liquid storage container is any one of glass and medical polymer materials.

10. The sampling device for detection of tumor cell pleuroperitoneal metastasis according to claim 1, characterized in that: The first connecting pipe and the second connecting pipe are made of medical polymer materials.

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