Selective enrichment culture medium for shiga toxin-producing Escherichia coli and application of selective enrichment culture medium

By optimizing the selective medium formula, combining ingredients such as streptidine, cefixime and magnesium sulfate heptahydrate, the problem of poor STEC inhibition and heterobacterial inhibition in the prior art is solved, and faster culture time and higher culture efficiency are achieved.

CN119685218APending Publication Date: 2025-03-25BEIJING YITAI BIOTECH CO LTD
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Patent Information

Application Number
CN202411902644.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-23
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

In the prior art, the inhibitory effect of selective culture medium on Shiga toxin Escherichia coli (STEC) is not obvious, and it is difficult to effectively inhibit the growth of miscellaneous bacteria, resulting in long culture time and low efficiency.

Method used

Optimize the selective culture medium formula, adopt components such as pancreatic casein peptone, succinic acid, sodium chloride, dipotassium hydrogen phosphate, glucose, etc., and reduce the inhibition of STEC and at the same time improve the inhibition of miscellaneous bacteria through the combination of selective antibacterial components such as lactis peptide, cefixime and magnesium sulfate heptahydrate.

Benefits of technology

The culture time of STEC is shortened, the culture efficiency is improved, the effective proliferation of STEC is ensured, and the growth of mixed bacteria is effectively inhibited.

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Abstract

The invention discloses a selective enrichment culture medium for shiga toxin-producing Escherichia coli and application of the selective enrichment culture medium. The culture medium comprises the following components in parts by mass: 18-19 parts of casein tryptone, 3-4 parts of succinic acid, 4.5-5.5 parts of sodium chloride, 3.5-4.5 parts of dipotassium phosphate, 1-2 parts of monopotassium phosphate, 3-4 parts of glucose, 0.000045-16 parts of a selective antibacterial component and 1000 parts of distilled water, the selective antibacterial component is one or a combination of more of magnesium sulfate heptahydrate, nisin and cefixime, and the pH value of the culture medium is 7.0-7.4. The culture medium provided by the invention can reduce the inhibition of selective components on shiga toxin-producing Escherichia coli, improve the inhibition on infectious microbes, shorten the culture time and improve the culture efficiency.
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