Lily carotenoid metabolism regulation gene LnCCD4a, protein, VIGS system and application
By introducing proteins encoded by the lily LnCCD4a gene and using the VIGS system to silencing the LnCCD4a gene, the color traits of lily flowers was regulated, and the problem of unstable transformation period of lily white flowers to yellow flowers was solved, and the transformation from white flowers to yellow flowers was achieved, and gene resources and technical basis were provided for lily color molecular breeding.
CN119685353APending Publication Date: 2025-03-25SHENYANG UNIV
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Patent Information
- Application Number
- CN202411600360.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-03-25
AI Technical Summary
Technical Problem
The prior art is difficult to effectively regulate the transformation of lily white flowers to yellow flowers, resulting in unstable cycles and limiting the molecular breeding of different lily colors.
Method used
By introducing proteins encoded by lily LnCCD4a gene, the LnCCD4a gene is silenced by VIGS system, and the color traits of lily flowers are regulated, so as to increase the carotenoid content in the flower quilt, thus achieving the transformation from white flowers to yellow flowers.
Benefits of technology
The carotenoid content in lily quilts has been successfully increased, the transformation from white flowers to yellow flowers has been achieved, and the genetic resources and technical basis for lily chromatic molecular breeding has been provided.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a lilium carotenoid metabolism regulation gene LnCCD4a, a protein, a VIGS system and application, and belongs to the technical field of gene engineering. VIGS experiments prove that the LnCCD4a gene has the function of regulating and controlling the flower color of the lily. A specific fragment is selected based on a lily endogenous LnCCD4a gene sequence, a silent expression vector is constructed, and a target gene is silent in a lily film by using a VIGS technology. Results show that after the LnCCD4a gene is silenced, the content of carotenoid in the flower tablets is increased, and the flower color is changed from white to yellow. The invention provides a gene resource and a technical basis for regulating the lily color through a molecular breeding technology.
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