Method and device for classifying and counting white blood cells based on morphology

Abstract

The invention belongs to the technical field of cell counting, and particularly relates to a method and device for classifying and counting white blood cells based on morphology, the device comprises a table body, the top of the table body is fixedly provided with an image microscopic collection mechanism, and the table body is slidably provided with a lifting frame along the vertical direction; the storage mechanism is arranged on the outer side of the table body; a loading / unloading mechanism; the cleaning mechanism is used for cleaning the image microscopic collection mechanism; and a lighting mechanism. According to the invention, the counting glass plate in the loading box can be moved to the unloading box after passing through the upper part of the image microscopic collection mechanism through the loading and unloading mechanism, so that the counting glass plate can be automatically loaded and unloaded, and the condition that the counting glass plate needs to be manually and repeatedly loaded and unloaded on equipment is reduced; and the top surface of the image microscopic collection mechanism can be cleaned through the cleaning mechanism along with loading and unloading of the loading and unloading mechanism, so that the situation that dust is attached to the image microscopic collection mechanism to affect cell image collection is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of cell counting, and in particular relates to a method and a device for counting white blood cells based on morphological classification. Background Art

[0002] Different types of white blood cells play different physiological functions, and different factors can cause changes in the number and morphology of white blood cells. Through morphological classification and counting, we can understand the number and proportion of each type of white blood cells, thereby evaluating the body's immune function and inflammatory status, which is of great significance for understanding the patient's physiological and pathological status, assisting clinical diagnosis, and formulating treatment plans.

[0003] Chinese patent publication number CN220603262U discloses a leukocyte classification counting device, which relates to the field of cell counting technology, including a stage, an image microscope collection device is fixedly connected to the inside of the stage, a lighting lamp is fixedly connected to one side of the upper end of the stage, a first frame is fixedly connected to the side of the lighting lamp on the stage, two first frames are provided, and a positioning assembly is fixedly installed on the two first frames. The utility model adopts the above structure, and by opening a trapezoidal counting chamber on the counting glass plate, the cells flow along the slope of the trapezoidal counting chamber until they gather at the bottom plane of the trapezoidal counting chamber, so that the cells are concentrated for convenient cell detection, and it is not easy to hide dirt. Moreover, since a light-transmitting glass plate is installed on the clamping plate, and the light-transmitting glass plate and the counting chamber are movably arranged, only the counting chamber needs to be cleaned, which reduces the subsequent cleaning workload, facilitates the cleaning of the counting chamber, and is cleaner after cleaning.

[0004] However, when the above-mentioned leukocyte classification and counting device is in use, the counting glass plate needs to be replaced manually. When counting the leukocytes in multiple counting glass plates, the counting glass plates need to be manually loaded and unloaded on the device repeatedly, which is not convenient for continuously counting the leukocytes in multiple counting glass plates. The convenience is insufficient, and the surface of the image microscopy collection device is easy to absorb dust, resulting in dust affecting the image microscopy collection device to collect cell images. Summary of the invention

[0005] The purpose of the present invention is to provide a method and device for counting white blood cells based on morphological classification, which can move the counting glass plate in the loading box to the unloading box after passing over the image microscope collection mechanism through the loading and unloading mechanism, so as to automatically load and unload the counting glass plate, thereby reducing the need to manually load and unload the counting glass plate on the equipment repeatedly, and can clean the top surface of the image microscope collection mechanism through the cleaning mechanism as the loading and unloading mechanism is loaded and unloaded, thereby reducing the situation where dust adheres to the image microscope collection mechanism and affects the collection of cell images.

[0006] The technical solution adopted by the present invention is as follows:

[0007] A device for counting white blood cells based on morphological classification, comprising:

[0008] A platform, the top of which is fixedly mounted with an image microscope collection mechanism, and a lifting frame is slidably mounted on the platform in a vertical direction;

[0009] A storage mechanism, the storage mechanism is arranged on the outside of the platform, the storage mechanism includes a loading box and an unloading box, the loading box and the unloading box are respectively fixedly connected to the two sides of the platform, a loading plate is slidably installed in the loading box along the vertical direction, and an unloading plate is slidably installed in the unloading box along the vertical direction, and a plurality of counting glass plates are arranged above the loading plate along the vertical direction, and the loading box and the unloading box are both slidably matched with the counting glass plates along the vertical direction;

[0010] A loading and unloading mechanism, the loading and unloading mechanism is arranged above the image microscopic collection mechanism, the loading and unloading mechanism comprises a slider slidably mounted on a lifting frame in a horizontal direction, a fixed clamping plate is fixedly connected to the slider, a lifting clamping plate is arranged on one side of the fixed clamping plate, the lifting clamping plate is slidably mounted on the slider in a vertical direction, and the fixed clamping plate and the lifting clamping plate are used to clamp the counting glass plate;

[0011] A cleaning mechanism, the cleaning mechanism is arranged on the top of the image microscopic collection mechanism and is used to clean the image microscopic collection mechanism. The cleaning mechanism includes an inclined slide fixedly connected to the platform body, the end of the inclined slide close to the loading box is higher than the end close to the unloading box, a cleaning roller is arranged above the image microscopic collection mechanism, the end of the cleaning roller is slidably connected to the inclined slide, a metal block is fixedly connected to the end of the cleaning roller close to the slider, an electromagnet is slidably connected to the bottom of the slider in a vertical direction, and the lower surface of the electromagnet fits with the upper surface of the metal block;

[0012] An illumination mechanism, the illumination mechanism being arranged above the loading and unloading mechanism, the illumination mechanism comprising a mounting plate slidably mounted on the lifting frame along a vertical direction, and an illumination component being fixedly mounted on a side of the mounting plate close to the image microscopic collection mechanism;

[0013] The slider drives the fixed clamp and the lifting clamp to clamp the counting glass plate and move it from the loading box to the top of the image microscope collection mechanism to collect cell images, and then moves to the unloading box to unload the counting glass plate. During the process of the slider returning from the unloading box to the loading box, it drives the cleaning roller to clean the top of the image microscope collection mechanism.

[0014] As a preferred embodiment of the device for counting white blood cells based on morphological classification described in the present invention, the counting glass plate includes a cover plate and a slide fitted together, and the cover plate and the slide are slidably connected to the outside with a ring sleeve, and the ring sleeve is used to limit the cover plate and the slide and to increase the vertical spacing between the cover plate and the slide between adjacent counting glass plates.

[0015] As a preferred solution of the device for counting white blood cells based on morphological classification described in the present invention, a first slide groove is provided on the inner wall of the loading box, and a second slide groove is provided on the inner wall of the unloading box. Both the first slide groove and the second slide groove are slidably matched with the ring sleeve in the vertical direction, and both the first slide groove and the second slide groove are slidably matched with the ring sleeve in the horizontal direction.

[0016] As a preferred embodiment of the device for counting white blood cells based on morphological classification described in the present invention, the loading plate is slidably connected to the loading box in the vertical direction, a first telescopic cylinder is fixedly connected to the inner side of the bottom of the loading box, and an output end of the first telescopic cylinder is fixedly connected to the bottom of the loading plate; the unloading plate is slidably connected to the unloading box in the vertical direction, a second telescopic cylinder is fixedly connected to the inner side of the bottom of the unloading box, and an output end of the second telescopic cylinder is fixedly connected to the bottom of the unloading plate.

[0017] As a preferred solution of the device for counting white blood cells based on morphological classification described in the present invention, the bottom of the slider is slidably connected to a lifting rod in the vertical direction, the lower end of the lifting rod is slidably matched with the top of the platform, and the electromagnet is fixedly connected to the lifting rod.

[0018] As a preferred solution of the device for counting white blood cells based on morphological classification described in the present invention, the upper end of the lifting rod is fixedly connected with a plurality of sliding bars along the circumferential direction, and the sliding bars slide and cooperate with the bottom of the slider along the vertical direction.

[0019] As a preferred embodiment of the device for counting white blood cells based on morphological classification described in the present invention, the lighting assembly includes a plurality of annular lighting lamps fixedly connected to the bottom of a mounting plate, the outer diameters of the plurality of annular lighting lamps are different and are concentrically arranged, and the plurality of annular lighting lamps are distributed on the mounting plate in a manner that the outer diameters increase from the inside to the outside.

[0020] As a preferred solution of the device for counting white blood cells based on morphological classification described in the present invention, wherein: the inner sides of the fixed splint and the lifting splint are fixedly connected with elastic blocks, and the surfaces of the elastic blocks are roughened.

[0021] As a preferred embodiment of the device for counting white blood cells based on morphological classification described in the present invention, the mounting plate is slidably connected to the lifting frame in the vertical direction, a first motor is fixedly connected to the lifting frame, a first screw is fixedly connected to the output end of the first motor, and the first screw is threadedly connected to the mounting plate.

[0022] The method for counting white blood cells based on morphological classification is applicable to the above-mentioned device for counting white blood cells based on morphological classification, and the specific method is as follows:

[0023] S1: First, a counting glass plate containing leukocytes is placed in a loading box, and after the loading and unloading mechanism is operated, the slider moves horizontally between the loading box and the unloading box;

[0024] S2: When the slider moves to the loading box, the fixed clamping plate and the lifting clamping plate clamp the counting glass plate for loading;

[0025] S3: When the slider moves to the top of the image microscopic collection mechanism, the image microscopic collection mechanism collects cell images from the counting glass plate, thereby counting white blood cells;

[0026] S4: When the slider moves to the unloading box, the fixed clamping plate and the lifting clamping plate no longer clamp the counting glass plate and unloading is carried out;

[0027] S5: The slider returns from the unloading box to the loading box for reloading. During this process, the slider drives the cleaning roller to move along the top of the image microscopic collection mechanism through the electromagnet to clean the image microscopic collection mechanism.

[0028] The technical effects achieved by the present invention are:

[0029] The present invention adopts the design of storage mechanism and loading and unloading mechanism. The loading and unloading mechanism can move the counting glass plate in the loading box to the unloading box after passing above the image microscopic collection mechanism, so as to automatically load and unload the counting glass plate. After the slider slides along the lifting frame, it drives the fixed clamping plate and the lifting clamping plate to move between the loading box and the unloading box. When moving to the loading box, the fixed clamping plate and the lifting clamping plate clamp the counting glass plate. When moving to the image microscopic collection mechanism, the image microscopic collection mechanism collects cell images of the counting glass plate. When moving to the unloading box, the fixed clamping plate and the lifting clamping plate release the counting glass plate, and then return to the loading box to be reloaded, so as to realize automatic loading and unloading, reduce the situation that the counting glass plate needs to be manually loaded and unloaded repeatedly on the equipment, and facilitate continuous counting of multiple counting glass plates.

[0030] The present invention adopts the design of a cleaning mechanism, and the cleaning mechanism can clean the top surface of the image microscopic collection mechanism as the loading and unloading mechanism is loaded and unloaded. When the slider returns from the unloading box to the loading box, the slider drives the electromagnet to move to the side close to the loading box. After the electromagnet is energized, it adsorbs the metal block, so that the slider drives the cleaning roller to move to the side close to the loading box. The cleaning roller moves along the inclined slide groove on the top surface of the image microscopic collection mechanism to clean the image microscopic collection mechanism, thereby reducing the situation where dust adheres to the image microscopic collection mechanism and affects the collection of cell images. After the cleaning roller cleans the image microscopic collection mechanism and moves to the side close to the loading box, the electromagnet is powered off and no longer adsorbs the metal block. The cleaning roller moves along the inclined slide groove to the side close to the unloading box under the action of its own gravity, thereby resetting the cleaning roller. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the structure of the present invention from an overall angle;

[0032] Figure 2 It is a schematic diagram of the structure of the present invention from another angle;

[0033] Figure 3 It is an exploded schematic diagram of the platform body and the loading and unloading mechanism in the present invention;

[0034] Figure 4 It is an exploded schematic diagram of the loading and unloading mechanism and the counting glass plate in the present invention;

[0035] Figure 5 It is an exploded schematic diagram of the unloading box and the counting glass plate in the present invention;

[0036] Figure 6 It is a structural schematic diagram of the platform and the cleaning mechanism in the present invention;

[0037] Figure 7 The present invention Figure 6 The enlarged schematic diagram of point A in the middle;

[0038] Figure 8 The present invention Figure 6 The enlarged schematic diagram of point B in the middle;

[0039] Fig. 9 It is a structural schematic diagram of the lighting assembly and the mounting plate in the present invention.

[0040] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0041] 10. platform; 11. image microscopic collection mechanism; 12. lifting frame; 20. storage mechanism; 21. loading box; 211. loading plate; 212. first slide; 213. first telescopic cylinder; 22. unloading box; 221. unloading plate; 222. second slide; 223. second telescopic cylinder; 23. counting glass plate; 231. cover plate; 232. carrier; 233. ring sleeve; 30. loading and unloading mechanism; 31. slider; 32. fixed splint; 33. lifting splint; 34. lifting rod; 341. slide bar; 35. elastic block; 40. cleaning mechanism; 41. inclined slide; 42. cleaning roller; 43. metal block; 44. electromagnet; 50. lighting mechanism; 51. mounting plate; 52. lighting assembly; 521. ring lighting lamp; 53. first motor; 54. first screw. DETAILED DESCRIPTION

[0042] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is specifically described below in conjunction with embodiments. It should be understood that the following text is only used to describe one or several specific embodiments of the present invention, and does not strictly limit the scope of protection of the specific claims of the present invention.

[0043] Example 1

[0044] like Figures 1 to 9As shown in the figure, it is the first embodiment of the present invention, which provides a device for counting white blood cells based on morphological classification, including a platform 10, a top of which is fixedly installed with an image microscopic collection mechanism 11, and a lifting frame 12 is slidably installed on the platform 10 in a vertical direction; a storage mechanism 20, which is arranged on the outside of the platform 10, and includes a loading box 21 and an unloading box 22, which are respectively fixedly connected to the two sides of the platform 10, and a loading box 21 is slidably installed in the loading box 21 along the vertical direction. The unloading box 22 is provided with a unloading plate 221 which is slidably mounted in the vertical direction. A plurality of counting glass plates 23 are arranged above the loading plate 211 in the vertical direction. The loading box 21 and the unloading box 22 are both slidably matched with the counting glass plates 23 in the vertical direction. The loading and unloading mechanism 30 is arranged above the image microscopic collection mechanism 11. The loading and unloading mechanism 30 includes a slider 31 which is slidably mounted on the lifting frame 12 in the horizontal direction. A fixed clamping plate 32 is fixedly connected to the slider 31. A lifting plate 32 is arranged on one side of the fixed clamping plate 32. The lowering clamping plate 33 is slidably installed on the slider 31 in the vertical direction, and the fixed clamping plate 32 and the lifting clamping plate 33 are used to clamp the counting glass plate 23; the cleaning mechanism 40 is arranged on the top of the image microscopic collection mechanism 11, and is used to clean the image microscopic collection mechanism 11. The cleaning mechanism 40 includes an inclined slide 41 fixedly connected to the platform 10, and the end of the inclined slide 41 close to the loading box 21 is higher than the end close to the unloading box 22. A cleaning roller 42 is arranged above the image microscopic collection mechanism 11, and the cleaning roller The end of 42 is slidably connected to the inclined slide groove 41, the end of the cleaning roller 42 close to the slider 31 is fixedly connected to the metal block 43, the bottom of the slider 31 is slidably connected to the electromagnet 44 along the vertical direction, and the lower surface of the electromagnet 44 fits with the upper surface of the metal block 43; the lighting mechanism 50, the lighting mechanism 50 is arranged above the loading and unloading mechanism 30, the lighting mechanism 50 includes a mounting plate 51 slidably installed on the lifting frame 12 along the vertical direction, and a lighting component 52 is fixedly installed on the side of the mounting plate 51 close to the image microscopy collection mechanism 11.

[0045] It should be noted that a counting information transmission module and an image classification processing module are also provided on the platform 10. The counting information transmission module and the image classification processing module are both existing technologies, and are used to process and transmit the cell images collected by the image microscopy collection mechanism 11 to classify and count white blood cells. They will not be repeated here. The lifting frame 12 is slidably connected to the platform 10 in the vertical direction. A third telescopic cylinder is fixedly connected to a side of the platform 10 close to the lifting frame 12. The output end of the third telescopic cylinder is fixedly connected to the lifting frame 12. A second screw is rotatably connected to a side of the lifting frame 12 close to the slider 31. The second screw is threadedly connected to the slider 31. A second motor is provided at one end of the second screw. The output end of the second motor is fixedly connected to the second screw. The second motor is fixedly connected to the lifting frame 12. The lifting and lowering splint 33 is fixedly connected, one end of the lifting and lowering splint 33 close to the slider 31 is slidably connected to the slider 31 along the vertical direction, the fixed splint 32 is fixedly connected to the fourth telescopic cylinder, the output end of the fourth telescopic cylinder is fixedly connected to the lifting and lowering splint 33, the mounting plate 51 is slidably connected to the lifting frame 12 along the vertical direction, the lifting frame 12 is fixedly connected to the third motor, the output end of the third motor is fixedly connected to the third screw, the third screw is threadedly connected to the mounting plate 51, and one side corner of the loading box 21 and the unloading box 22 close to the slider 31 is opened for the fixed splint 32 and the lifting and lowering splint 33 to pass through the opening to enter and exit the loading box 21 and the unloading box 22 to take and place the counting glass plate 23, the cleaning roller 42 is made of elastic material, and the end of the cleaning roller 42 is rotatably matched with the inclined slide groove 41.

[0046] When the present invention is in use, the slider 31 slides horizontally on the lifting frame 12. When the slider 31 drives the fixed clamping plate 32 and the lifting clamping plate 33 to move to the object box 21, the lifting clamping plate 33 slides along the slider 31 and approaches the fixed clamping plate 32, so that the fixed clamping plate 32 and the lifting clamping plate 33 clamp the counting glass plate 23. The slider 31 drives the counting glass plate 23 to be taken out of the object box 21 and moved to the top of the image microscopic collection mechanism 11 through the fixed clamping plate 32 and the lifting clamping plate 33. The lighting assembly 52 in the lighting mechanism 50 is The image microscopic collection mechanism 11 provides illumination, and the image microscopic collection mechanism 11 collects cell images of the counting glass plate 23, so as to facilitate the counting of white blood cells. When the slider 31 drives the counting glass plate 23 to move to the unloading box 22 through the fixed clamping plate 32 and the lifting clamping plate 33, the lifting clamping plate 33 slides along the slider 31 and moves away from the fixed clamping plate 32, so that the fixed clamping plate 32 and the lifting clamping plate 33 no longer clamp the counting glass plate 23, so that the counting glass plate 23 is unloaded from the unloading box 22, and the slider 31 drives the fixed clamping plate 32 and the lifting clamping plate 33 to move to the unloading box 22. The clamping plate 33 returns to the loading box 21 to take out another counting glass plate 23, thereby realizing automatic loading and unloading of the counting glass plate 23, reducing the need to manually repeatedly load and unload the counting glass plate 23 on the equipment, facilitating continuous counting of multiple counting glass plates 23, and improving convenience. When the slider 31 returns, it drives the electromagnet 44 to pass through the metal block 43. After the electromagnet 44 is powered on, it absorbs the metal block 43, so that the slider 31 and the cleaning roller 42 move together to the side close to the loading box 21, so that the cleaning roller 42 moves along the image display The micro-collection mechanism 11 moves and cleans the top surface of the image microscopic collection mechanism 11 to reduce the dust adhering to the image microscopic collection mechanism 11 and affecting the collection of cell images. When the slider 31 drives the cleaning roller 42 to move to the side of the image microscopic collection mechanism 11 close to the loading box 21, the electromagnet 44 is powered off and no longer absorbs the metal block 43. Since the inclined slide 41 is inclined, the cleaning roller 42 moves along the inclined slide 41 to the side close to the unloading box 22 under the action of its own gravity, thereby resetting the cleaning roller 42.

[0047] Among them, when the slider 31 drives the fixed splint 32 and the lifting splint 33 to take out the counting glass plate 23 in the loading box 21, the loading plate 211 moves upward along the loading box 21 and pushes the uppermost counting glass plate 23 in the loading box 21 to move upward, so that the counting glass plate 23 extends out of the top of the loading box 21, which is convenient for the fixed splint 32 and the lifting splint 33 to clamp the counting glass plate 23. After the fixed splint 32 and the lifting splint 33 unload the counting glass plate 23 at the unloading box 22, the unloading plate 221 moves downward along the unloading box 22, so that the unloading plate 221 drives the counting glass plate 23 above the unloading plate 221 to move downward along the unloading box 22 and be stored in the inside of the unloading box 22, leaving space for the subsequent fixed splint 32 and the lifting splint 33 to unload another counting glass plate 23.

[0048] Example 2

[0049] Reference Figures 1 to 9 , which is the second embodiment of the present invention, and this embodiment is based on the previous embodiment.

[0050] like Figure 4 and Figure 5 As shown, the counting glass plate 23 includes a cover plate 231 and a carrier plate 232 which are attached to each other from top to bottom. The cover plate 231 and the carrier plate 232 are externally slidably connected with a ring sleeve 233, which is used to limit the cover plate 231 and the carrier plate 232 and to increase the vertical spacing between the cover plate 231 and the carrier plate 232 between adjacent counting glass plates 23.

[0051] It should be noted that when the fixed clamping plate 32 and the lifting clamping plate 33 clamp the counting glass plate 23, the ends of the fixed clamping plate 32 and the lifting clamping plate 33 are both in contact with the ring sleeve 233 to reduce the rotation of the counting glass plate 23 during the clamping process.

[0052] According to the above structure, when the counting glass plates 23 are stacked in the loading box 21 and the unloading box 22 in the vertical direction, the ring sleeves 233 between the two adjacent counting glass plates 23 fit together, so that the cover plate 231 and the carrier plate 232 between the two adjacent counting glass plates 23 are separated and a gap is formed, which is convenient for the fixing clamp 32 and the lifting clamp 33 to be inserted into the gap to clamp the cover plate 231 and the carrier plate 232, thereby clamping the counting glass plate 23.

[0053] like Figure 5 As shown, a first slide groove 212 is opened on the inner wall of the loading box 21, and a second slide groove 222 is opened on the inner wall of the unloading box 22. The first slide groove 212 and the second slide groove 222 are both slidably matched with the ring sleeve 233 in the vertical direction, and the first slide groove 212 and the second slide groove 222 are both slidably matched with the ring sleeve 233 in the horizontal direction.

[0054] It should be noted that a first pad is fixedly connected to the top of the loading plate 211, and the first pad slides in cooperation with the first slide groove 212 in the vertical direction, so as to separate the loading plate 211 and the carrier 232 and form a gap, so as to facilitate the insertion of the lifting clamp 33 into the gap; a second pad is fixedly connected to the top of the unloading plate 221, and the second pad slides in cooperation with the second slide groove 222 in the vertical direction, so as to separate the loading plate 211 and the carrier 232 and form a gap, so as to facilitate the insertion of the lifting clamp 33 into the gap.

[0055] According to the above structure, the first slide groove 212 and the second slide groove 222 are both slidably cooperated with the ring sleeve 233 in the vertical direction, which is convenient for the counting glass plate 23 to slide in the vertical direction in the loading box 21 and the unloading box 22, so that the loading board 211 drives the counting glass plate 23 in the loading box 21 to move up and take it out, and the unloading board 221 drives the counting glass plate 23 in the unloading box 22 to move down and store it. The first slide groove 212 and the second slide groove 222 are both slidably cooperated with the ring sleeve 23 in the horizontal direction, which is convenient for the fixing splint 32 and the lifting splint 33 to clamp the counting glass plate 23 from the loading box 21 and make the counting glass plate 23 slide out along the loading box 21, and the fixing splint 32 and the lifting splint 33 slide the counting glass plate 23 to be placed in the unloading box 22.

[0056] like Figure 5 As shown, the loading plate 211 is slidably connected to the loading box 21 in the vertical direction, a first telescopic cylinder 213 is fixedly connected to the inner side of the bottom of the loading box 21, and an output end of the first telescopic cylinder 213 is fixedly connected to the bottom of the loading plate 211, and the unloading plate 221 is slidably connected to the unloading box 22 in the vertical direction, a second telescopic cylinder 223 is fixedly connected to the inner side of the bottom of the unloading box 22, and an output end of the second telescopic cylinder 223 is fixedly connected to the bottom of the unloading plate 221.

[0057] According to the above structure, after the first telescopic cylinder 213 works, the output end drives the loading plate 211 to slide up and down in the loading box 21 along the vertical direction, so as to adjust the height position of the uppermost counting glass plate 23 in the loading box 21, and facilitate the fixing clamping plate 32 and the lifting clamping plate 33 to clamp the counting glass plate 23 from the loading box 21. After the second telescopic cylinder 223 works, the output end drives the unloading plate 221 to slide up and down in the unloading box 22 along the vertical direction, so as to adjust the height position of the uppermost counting glass plate 23 in the unloading box 22, and facilitate to reserve space for the fixing clamping plate 32 and the lifting clamping plate 33 to place the counting glass plate 23 in the unloading box 22.

[0058] like Figure 7 As shown, the bottom of the slider 31 is slidably connected to a lifting rod 34 in the vertical direction, the lower end of the lifting rod 34 is slidably matched with the top of the platform 10, and the electromagnet 44 is fixedly connected to the lifting rod 34.

[0059] It should be noted that the electromagnet 44 is a prior art, which converts electrical energy into magnetic energy by using the principle that electric current generates a magnetic field through a coil, thereby generating a magnetic force to attract metal or other magnetic materials, which is used to attract the metal block 43. It will not be repeated here. The lower end of the lifting rod 34 is made into an arc to facilitate the lower end of the lifting rod 34 to slide on the top of the platform 10. When the metal block 43 is located at one end of the inclined slide 41 close to the unloading box 22, the lower end of the lifting rod 34 is in contact with the top surface of the platform 10, and the upper surface of the metal block 43 and the lower surface of the electromagnet 44 are located on the same horizontal plane.

[0060] According to the above structure, in the process that the slider 31 drives the electromagnet 44 to move from the side close to the unloading box 22 to the side close to the loading box 21 through the lifting rod 34, the electromagnet 44 attracts the metal block 43 after being energized and drives the metal block 43 and the cleaning roller 42 to move together. Since the inclined slide groove 41 is inclined, the height position of the electromagnet 44 and the metal block 43 rises during the movement, so that the lifting rod 34 moves upward along the slider 31. When the cleaning roller 42 moves to the side close to the loading box 21 and needs to be reset, the electromagnet 44 no longer attracts the metal block 43 after being powered off. The metal block 43 is separated from the electromagnet 44. The cleaning roller 42 moves along the inclined slide groove 41 to the side close to the unloading box 22 under the action of its own gravity, so that the height position of the metal block 43 decreases. The electromagnet 44 and the lifting rod 34 move downward along the slider 31 under the action of their own gravity and the height position decreases, thereby achieving reset.

[0061] like Figure 7 As shown, the upper end of the lifting rod 34 is fixedly connected with a plurality of slide bars 341 along the circumferential direction, and the slide bars 341 are slidably matched with the bottom of the sliding block 31 along the vertical direction.

[0062] According to the above structure, the slide bar 341 cooperates with the slider 31 to reduce the rotation of the lifting rod 34 relative to the slider 31, so that the lifting rod 34 and the electromagnet 44 move up and down along the slider 31 more stably.

[0063] like Fig. 9 As shown, the lighting assembly 52 includes a plurality of annular lighting lamps 521 fixedly connected to the bottom of the mounting plate 51. The outer diameters of the plurality of annular lighting lamps 521 are different and are concentrically arranged. The plurality of annular lighting lamps 521 are distributed on the mounting plate 51 in a manner that the outer diameters increase from the inside to the outside.

[0064] It should be noted that the switches of the plurality of annular lighting lamps 521 are independent of each other and are used to independently switch on and off each annular lighting lamp 521 . This is an existing mature technology and will not be described in detail here.

[0065] According to the above structure, by adjusting the opening and closing of several ring-shaped lighting lamps 521, it is convenient to adjust the overall illumination area and illumination intensity of the illumination assembly 52, thereby facilitating the adjustment of the illumination of the counting glass plate 23 by the illumination assembly 52, and further facilitating the image microscope collection mechanism 11 to collect cell images of the counting glass plate 23.

[0066] like Figure 4 As shown, the inner sides of the fixed clamping plate 32 and the lifting clamping plate 33 are fixedly connected with elastic blocks 35, and the surfaces of the elastic blocks 35 are roughened.

[0067] It should be noted that the elastic block 35 is made of elastic material and is used to reduce the damage to the counting glass plate 23 when the fixing clamping plate 32 and the lifting clamping plate 33 clamp the counting glass plate 23 .

[0068] According to the above structure, when the fixed clamp 32 and the lifting clamp 33 clamp the counting glass plate 23, the elastic block 35 with a rough surface increases the friction between the counting glass plate 23 and the elastic block 35, thereby reducing the loosening of the counting glass plate 23 when clamping the counting glass plate 23.

[0069] like Figure 2 As shown, the mounting plate 51 is slidably connected to the lifting frame 12 along the vertical direction, a first motor 53 is fixedly connected to the lifting frame 12, a first screw 54 is fixedly connected to the output end of the first motor 53, and the first screw 54 is threadedly connected to the mounting plate 51.

[0070] According to the above structure, after the first motor 53 works, the output end drives the first screw 54 to rotate, and the first screw 54 drives the mounting plate 51 to move up and down in the vertical direction on the lifting frame 12, so as to adjust the distance between the lighting component 52 and the counting glass plate 23, so as to adjust the illumination of the counting glass plate 23 by the lighting component 52, and then facilitate the adjustment of the illumination of the counting glass plate 23 by the image microscopy collection mechanism 11 to collect cell images, so as to facilitate the image microscopy collection mechanism 11 to collect cell images.

[0071] In order to enable those skilled in the art to have a further understanding of the device for counting white blood cells based on morphological classification of the present invention, the present invention also provides a method for counting white blood cells based on morphological classification, which is applicable to the above-mentioned device for counting white blood cells based on morphological classification, and the specific method is:

[0072] S1: First, the counting glass plate 23 containing white blood cells is placed in the loading box 21, and the loading and unloading mechanism 30 is operated to make the slider 31 move horizontally between the loading box 21 and the unloading box 22;

[0073] S2: When the slider 31 moves to the loading box 21, the fixed clamping plate 32 and the lifting clamping plate 33 clamp the counting glass plate 23 for loading;

[0074] S3: When the slider 31 moves to the top of the image microscopic collection mechanism 11, the image microscopic collection mechanism 11 collects cell images from the counting glass plate 23, thereby counting white blood cells;

[0075] S4: When the slider 31 moves to the unloading box 22, the fixed clamping plate 32 and the lifting clamping plate 33 no longer clamp the counting glass plate 23, and unloading is performed;

[0076] S5: The slider 31 returns from the unloading box 22 to the loading box 21 for reloading. During this process, the slider 31 drives the cleaning roller 42 to move along the top of the image microscopic collection mechanism 11 through the electromagnet 44 to clean the image microscopic collection mechanism 11.

[0077] The working principle of the present invention is as follows: when the counting glass plate 23 needs to be installed, the slider 31 drives the fixed clamping plate 32 and the lifting clamping plate 33 to move to the loading box 21 and clamp the counting glass plate 23, the slider 31 drives the counting glass plate 23 to move to the top of the image microscopic collection mechanism 11 through the fixed clamping plate 32 and the lifting clamping plate 33, and the image microscopic collection mechanism 11 collects cell images of the counting glass plate 23, thereby realizing the installation of the counting glass plate 23, which is convenient for counting white blood cells, and when the counting glass plate 23 needs to be unloaded, the slider 31 drives the counting glass plate 23 to move to the unloading box 22 through the fixed clamping plate 32 and the lifting clamping plate 33, the fixed clamping plate 32 and the lifting clamping plate 33 no longer clamp the counting glass plate 23, and the counting glass plate 23 is stored in the unloading box 2 2, the counting glass plate 23 is removed, and the slider 31 drives the fixed clamping plate 32 and the lifting clamping plate 33 to return to the loading box 21 to reinstall the counting glass plate 23, thereby realizing automatic loading and unloading of the counting glass plate 23, reducing the need to manually load and unload the counting glass plate 23 on the equipment repeatedly, and facilitating continuous counting of multiple counting glass plates 23. In the process of the slider 31 returning to the loading box 21, the electromagnet 44 drives the metal block 43 and the cleaning roller 42 to move, so that the cleaning roller 42 moves along the top surface of the image microscopic collection mechanism 11 and cleans the image microscopic collection mechanism 11, reducing the situation where dust adheres to the image microscopic collection mechanism 11 and affects the collection of cell images, and facilitating the image microscopic collection mechanism 11 to collect cell images.

[0078] The above is only a preferred embodiment of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principles of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the art unless otherwise specified and limited.

Claims

1. A device for counting white blood cells based on morphological classification, characterized in that: include: A platform (10), wherein an image microscopic collection mechanism (11) is fixedly mounted on the top of the platform (10), and a lifting frame (12) is slidably mounted on the platform (10) in a vertical direction; A storage mechanism (20), the storage mechanism (20) being arranged on the outside of the platform (10), the storage mechanism (20) comprising a loading box (21) and an unloading box (22), the loading box (21) and the unloading box (22) being respectively fixedly connected to two sides of the platform (10), a loading plate (211) being slidably mounted in the loading box (21) along the vertical direction, and an unloading plate (221) being slidably mounted in the unloading box (22) along the vertical direction, a plurality of counting glass plates (23) being arranged above the loading plate (211) along the vertical direction, and the loading box (21) and the unloading box (22) both being slidably matched with the counting glass plates (23) along the vertical direction; A loading and unloading mechanism (30), the loading and unloading mechanism (30) is arranged above the image microscopic collection mechanism (11), the loading and unloading mechanism (30) comprises a slider (31) slidably mounted on a lifting frame (12) in a horizontal direction, a fixed clamping plate (32) is fixedly connected to the slider (31), a lifting clamping plate (33) is arranged on one side of the fixed clamping plate (32), the lifting clamping plate (33) is slidably mounted on the slider (31) in a vertical direction, and the fixed clamping plate (32) and the lifting clamping plate (33) are used to clamp the counting glass plate (23); A cleaning mechanism (40), the cleaning mechanism (40) being arranged on the top of the image microscopic collection mechanism (11) and being used for cleaning the image microscopic collection mechanism (11), the cleaning mechanism (40) comprising an inclined slide groove (41) fixedly connected to the platform (10), the end of the inclined slide groove (41) close to the loading box (21) being higher than the end close to the unloading box (22), a cleaning roller (42) being arranged above the image microscopic collection mechanism (11), the end of the cleaning roller (42) being slidably connected to the inclined slide groove (41), the end of the cleaning roller (42) close to the slider (31) being fixedly connected to a metal block (43), the bottom of the slider (31) being slidably connected to an electromagnet (44), the lower surface of the electromagnet (44) being fitted with the upper surface of the metal block (43); an illumination mechanism (50), the illumination mechanism (50) being arranged above the loading and unloading mechanism (30), the illumination mechanism (50) comprising a mounting plate (51) slidably mounted on the lifting frame (12) in a vertical direction, a lighting assembly (52) being fixedly mounted on a side of the mounting plate (51) close to the image microscopic collection mechanism (11); The slider (31) drives the fixed clamp (32) and the lifting clamp (33) to clamp the counting glass plate (23) and move it from the loading box (21) to the top of the image microscope collection mechanism (11) to collect cell images, and then moves to the unloading box (22) to unload the counting glass plate (23). In the process of the slider (31) returning from the unloading box (22) to the loading box (21), it drives the cleaning roller (42) to clean the top of the image microscope collection mechanism (11).

2. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The counting glass plate (23) comprises a cover plate (231) and a carrier plate (232) which are bonded together. The cover plate (231) and the carrier plate (232) are slidably connected to the outside with a ring sleeve (233). The ring sleeve (233) is used to limit the cover plate (231) and the carrier plate (232) and to increase the vertical spacing between the cover plate (231) and the carrier plate (232) between adjacent counting glass plates (23).

3. The device for counting white blood cells based on morphological classification according to claim 2, characterized in that: A first slide groove (212) is provided on the inner wall of the loading box (21), and a second slide groove (222) is provided on the inner wall of the unloading box (22). The first slide groove (212) and the second slide groove (222) are both slidably matched with the ring sleeve (233) in the vertical direction, and the first slide groove (212) and the second slide groove (222) are both slidably matched with the ring sleeve (233) in the horizontal direction.

4. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The loading plate (211) is slidably connected to the loading box (21) in a vertical direction; a first telescopic cylinder (213) is fixedly connected to the inner side of the bottom of the loading box (21); an output end of the first telescopic cylinder (213) is fixedly connected to the bottom of the loading plate (211); the unloading plate (221) is slidably connected to the unloading box (22) in a vertical direction; a second telescopic cylinder (223) is fixedly connected to the inner side of the bottom of the unloading box (22); an output end of the second telescopic cylinder (223) is fixedly connected to the bottom of the unloading plate (221).

5. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The bottom of the slider (31) is slidably connected to a lifting rod (34) in a vertical direction, the lower end of the lifting rod (34) is slidably matched with the top of the platform (10), and the electromagnet (44) is fixedly connected to the lifting rod (34).

6. The device for counting white blood cells based on morphological classification according to claim 5, characterized in that: The upper end of the lifting rod (34) is fixedly connected to a plurality of sliding bars (341) along the circumferential direction, and the sliding bars (341) are slidably matched with the bottom of the sliding block (31) along the vertical direction.

7. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The lighting assembly (52) comprises a plurality of annular lighting lamps (521) fixedly connected to the bottom of the mounting plate (51); the outer diameters of the plurality of annular lighting lamps (521) are different and are arranged concentrically; the plurality of annular lighting lamps (521) are distributed on the mounting plate (51) in a manner such that the outer diameters increase in sequence from the inside to the outside.

8. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The inner sides of the fixed clamping plate (32) and the lifting clamping plate (33) are both fixedly connected with elastic blocks (35), and the surfaces of the elastic blocks (35) are roughened.

9. The device for counting white blood cells based on morphological classification according to claim 1, characterized in that: The mounting plate (51) is slidably connected to the lifting frame (12) in a vertical direction; a first motor (53) is fixedly connected to the lifting frame (12); an output end of the first motor (53) is fixedly connected to a first screw rod (54); and the first screw rod (54) is threadedly connected to the mounting plate (51).

10. A method for counting white blood cells based on morphological classification, characterized in that: The method is applicable to the device for counting white blood cells based on morphological classification as described in any one of claims 1 to 9, and the specific method is: S1: First, a counting glass plate (23) containing white blood cells is placed in a loading box (21), and after the loading and unloading mechanism (30) is operated, the slider (31) moves horizontally between the loading box (21) and the unloading box (22); S2: When the slider (31) moves to the loading box (21), the fixed clamping plate (32) and the lifting clamping plate (33) clamp the counting glass plate (23) to load the material; S3: When the slider (31) moves to the top of the image microscopic collection mechanism (11), the image microscopic collection mechanism (11) collects cell images from the counting glass plate (23), thereby counting white blood cells; S4: When the slider (31) moves to the unloading box (22), the fixed clamping plate (32) and the lifting clamping plate (33) no longer clamp the counting glass plate (23) and unloading is performed; S5: The slider (31) returns from the unloading box (22) to the loading box (21) to be reloaded. During this process, the slider (31) drives the cleaning roller (42) to move along the top of the image microscopic collection mechanism (11) through the electromagnet (44) to clean the image microscopic collection mechanism (11).

Images