Cutting seedling raising method for lygodium japonicum

Abstract

The invention discloses a cutting seedling raising method for lygodium japonicum, and relates to the technical field of lygodium japonicum cultivation. Comprising the following steps: 1) a pretreatment stage; 2) cutting material preparation; (3) preparing a cutting medium and a container; 4) cutting operation; 5) controlling illumination and humidity; and 6) observation and management. By means of artificial environment control and specific hormone treatment, efficient and rapid propagation of the excellent variety of the frozen green in the artificial low-light environment is achieved, and the requirement for annual commercial-scale production is met.

Description

Technical Field

[0001] The invention relates to the technical field of freeze-green cultivation, and more particularly to a freeze-green cutting seedling cultivation method. Background Art

[0002] Frozen green (Rhamnus utilis Decne.) is a shrub or small tree of the genus Rhamnus in the Rhamnaceae family, which can reach a height of 4 meters. It likes light, tolerates shade, has strong adaptability, likes to grow on fertile and well-drained soil, is also tolerant to barrenness, and has strong cold resistance. It is produced in Guandi Mountain, Qinyuan County, Yuanqu County, Yicheng County, Qinshui County and other places in our province, and grows under mountain shrubs or sparse forests at an altitude of 700-2100 meters. The leaves have the effects of clearing heat and dampness, eliminating accumulation and relieving constipation. Wild frozen green leaf tea enjoys the reputation of "King of Medicinal Tea". According to tests, the content of total flavonoids in Lvliang frozen green leaves is as high as 4.2%. Studies have shown that the flavonoid extraction amount of frozen green fruit can reach 62.36mg / g by ultrasonic assisted ethanol extraction method, and the flavonoid extraction amount of frozen green leaves can reach 35mg / g. Flavonoids have obvious effects on preventing cell aging, improving blood circulation, and lowering cholesterol. The seeds contain a large amount of fatty oil and protein, which can be pressed to make lubricating oil and soap. As a plant with wide application value, frozen green has important applications in medicine, food and industry.

[0003] However, the large-scale production of frozen green is restricted by the propagation technology of excellent varieties. The existing cutting technology has not been effectively applied to frozen green, and this problem has become a key bottleneck restricting its industrial development. Traditional full-light cuttings need to be carried out outdoors and sprayed with water regularly, which has the disadvantages of seasonal restrictions and waste of water resources.

[0004] Therefore, providing a low-light-intensity mist frozen green cutting technology is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention provides a freeze-green cutting seedling method.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A frozen green cutting seedling raising method comprises the following steps:

[0008] 1) Preprocessing stage:

[0009] Select the frozen green plants collected outdoors and transfer them indoors for adaptive cultivation; the plants are adapted for 5 days in an artificial environment;

[0010] 2) Preparation of cutting materials:

[0011] Select healthy, pest-free, non-lignified frozen green stem segments with a length of 10 to 15 cm; cut the stem segments into an oblique surface, leaving 2 to 3 leaves on the top; soak the stem segments in solution A for 1 hour;

[0012] 3) Cutting medium and container configuration:

[0013] Use a mixture of vermiculite and perlite in a container with an open top and drainage holes at the bottom.

[0014] 4) Cutting operation:

[0015] Insert the treated stem segment into the substrate to a depth of 1 / 3 of the stem segment length;

[0016] 5) Light and humidity control:

[0017] Add solution B to the ultrasonic humidifier and use atomization technology to maintain the humidity in the container; set up an LED full-spectrum plant light 10 cm above the plant to provide a lighting environment;

[0018] 6) Observation and management:

[0019] The environmental conditions were maintained and the growth of the plants was observed.

[0020] Furthermore, in step 1), the artificial environment has a light intensity of 200 μmol / m 2 ·s -1 , relative humidity 70%, day temperature 25℃, night temperature 15℃.

[0021] Furthermore, the formula of solution A in step 2) is: 12000 ppm of indolebutyric acid (IBA); 1000 ppm of naphthylacetic acid (NAA); 10000 ppm of sucrose; 25-125 ppm of nano zinc (ZnO NPs); 10-60 ppm of nano silver (AgNPs); 15-70 ppm of nano iron oxide (Fe3O4 NPs); and the rest is water.

[0022] Furthermore, the formula of solution A is: 12000 ppm indolebutyric acid (IBA); 1000 ppm naphthylacetic acid (NAA); 10000 ppm sucrose; 75 ppm nano zinc (ZnO NPs); 35 ppm nano silver (AgNPs); 45 ppm nano iron oxide (Fe3O4 NPs); and the rest is water.

[0023] Furthermore, in step 3), the volume ratio of vermiculite to perlite is 1:1.

[0024] Furthermore, in step 5), the illumination intensity in the illumination environment is 200 μmol / m 2 ·s -1 ;

[0025] The formula of the B solution is polyethylene glycol (PEG) 5000-10000ppm; copper sulfate 500ppm; Tween 100ppm, methyl jasmonate 10-30ppm, and the rest is water.

[0026] Furthermore, the formula of the B solution is 8000ppm of polyethylene glycol (PEG); 500ppm of copper sulfate; 100ppm of Tween, 20ppm of methyl jasmonate, and the rest is water.

[0027] It can be seen from the above technical solution that, compared with the prior art, the beneficial effects of the present invention are:

[0028] The present invention realizes efficient and rapid propagation of frozen green fine varieties in an artificial low-light environment through artificial environmental control and specific hormone treatment, so as to meet the needs of commercial-scale production throughout the year.

[0029] The invention solves the technical difficulties of frozen green cutting seedling cultivation, improves the survival rate of frozen green cutting seedling cultivation, shortens the seedling cultivation period compared with traditional grafting seedling cultivation, and has important practical and popularization significance in the reproduction and production of frozen green seedlings.

[0030] The present invention is expected to be able to reproduce a large number of high-quality frozen green seedlings in a short period of time, providing strong technical support for the commercial planting of frozen green. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0032] Figure 1 The accompanying drawings show the growth conditions of Examples 1-3 of the present invention after one week, Example 1 (left), Example 2 (middle), Example 3 (right);

[0033] Figure 2 The accompanying drawings show the growth conditions of Examples 1-3 of the present invention after two weeks, Example 1 (left), Example 2 (middle), Example 3 (right);

[0034] Figure 3 The accompanying drawing shows the growth of comparative example 1 of the present invention after one week;

[0035] Figure 4 The accompanying figure shows the growth of comparative example 2 of the present invention after one week;

[0036] Figure 5The accompanying figure shows the growth of comparative example 3 of the present invention after one week;

[0037] Figure 6 The accompanying figure shows the growth condition of comparative example 4 of the present invention after 25 days of full light treatment. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Example 1

[0040] 1. A frozen green cutting seedling method, comprising the following steps:

[0041] 1) Preprocessing stage:

[0042] The frozen green plants were selected from the outdoor collection and transferred indoors for adaptive cultivation; in an artificial environment (light intensity 200 μmol / m 2 ·s -1 , relative humidity 70%, day temperature 25°C, night temperature 15°C) to carry out 5 days of adaptation cultivation of the plants;

[0043] 2) Preparation of cutting materials:

[0044] Select healthy, pest-free, non-lignified frozen green stem segments with a length of 10 cm to 15 cm. Cut the stem segments into an inclined surface, retaining 2-3 leaves on the top; soak the stem segments in solution A for 1 hour; the solution A formula is indolebutyric acid (IBA) 12000ppm; naphthaleneacetic acid (NAA) 1000ppm; sucrose 10000ppm; nano zinc (ZnO NPs) 75ppm; nano silver (AgNPs) 35ppm; nano iron oxide (Fe3O4NPs) 45ppm; the rest is water;

[0045] 3) Cutting medium and container configuration:

[0046] Use a substrate made by mixing vermiculite and perlite in a volume ratio of 1:1; put the substrate into a container with an opening at the top; leave drainage holes at the bottom of the container.

[0047] 4) Cutting operation:

[0048] Insert the treated stem segment into the substrate to a depth of 1 / 3 of the stem segment length.

[0049] 5) Light and humidity control:

[0050] Add solution B to the ultrasonic humidifier and use atomization technology to maintain the humidity in the container. Set up a full-spectrum LED plant light 10 cm above the plant to provide sufficient light, with a light intensity of 200 μmol / m 2 ·s -1 Solution B is polyethylene glycol (PEG) 8000ppm; copper sulfate 500ppm; Tween 100ppm, methyl jasmonate: 20ppm, and the rest is water;

[0051] 6) Observation and management:

[0052] The environmental conditions were maintained and the growth of the plants was observed. After about 1 week, the stem segments successfully took root.

[0053] Example 2

[0054] The formula of solution A is 12000ppm of indolebutyric acid (IBA); 1000ppm of naphthaleneacetic acid (NAA); 10000ppm of sucrose; 25ppm of nano zinc (ZnO NPs); 10ppm of nano silver (AgNPs); 15ppm of nano iron oxide (Fe3O4NPs); and the rest is water.

[0055] Solution B is polyethylene glycol (PEG) 5000ppm; copper sulfate 500ppm; Tween 100ppm, methyl jasmonate: 10ppm, and the rest is water;

[0056] The remaining steps are the same as in Example 1.

[0057] Example 3

[0058] The formula of solution A is 12000ppm of indolebutyric acid (IBA); 1000ppm of naphthaleneacetic acid (NAA); 10000ppm of sucrose; 125ppm of nano zinc (ZnO NPs); 60ppm of nano silver (AgNPs); 70ppm of nano iron oxide (Fe3O4NPs); and the rest is water.

[0059] Solution B is polyethylene glycol (PEG) 10000ppm; copper sulfate 500ppm; Tween 100ppm, methyl jasmonate: 30ppm, and the rest is water;

[0060] The remaining steps are the same as in Example 1.

[0061] Comparative Example 1

[0062] The formula of solution A is 12000ppm of indolebutyric acid (IBA); 1000ppm of naphthaleneacetic acid (NAA); 10000ppm of sucrose; and the rest is water;

[0063] The remaining steps are the same as in Example 1.

[0064] Comparative Example 2

[0065] The formula of solution A is 12000ppm of indolebutyric acid (IBA); 1000ppm of naphthaleneacetic acid (NAA); the rest is water;

[0066] The remaining steps are the same as in Example 1.

[0067] Comparative Example 3

[0068] A solution formula is water;

[0069] The remaining steps are the same as in Example 1.

[0070] Table 1 Effects of different rooting agent formulas on the growth of frozen green cuttings

[0071] Average rooting rate / % Mean root length (mm) Average number of roots Example 1 98.3±6.2 10.6±0.53 10 Example 2 95.3±8.9. 8.2±0.9 3 Example 3 94.5±7.8 7.6±0.32 7 Comparative Example 1 82.3±5.7 5±0.45 4 Comparative Example 2 78.6±3.4 3.1±0.26 3 Comparative Example 3 0 0 0

[0072] From Table 1, Figure 1-Figure 5 It can be seen that different rooting agent formulas can make frozen green cuttings take root, among which Example 1 has the best effect, with an average rooting rate of 98.3%, which is 1.2 times that of Comparative Example 1 and 1.25 times that of Comparative Example 2, and no rooting in Comparative Example 3. Example 1 has the highest average root length, which is 10.6 mm, which is 2.12 times that of Comparative Example 1 and 3.23 times that of Comparative Example 2. Example 1 also has the highest average number of roots, which is 10, which is 2.5 times that of Comparative Example 1 and 3.3 times that of Comparative Example 2.

[0073] Comparative Example 4

[0074] Conventional cuttings, natural environment, full light, rooting agent formula and treatment are the same as those in Example 1.

[0075] Depend on Figure 6 It can be seen that the rooting time of Comparative Example 4 is about 25 days, and no rooting will occur if no rooting agent is used.

[0076] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A frozen green cutting seedling method, characterized in that: The following steps are involved: 1) Preprocessing stage: Select the frozen green plants collected outdoors and transfer them indoors for adaptive cultivation; the plants are adapted for 5 days in an artificial environment; 2) Preparation of cutting materials: Select healthy, pest-free, non-lignified frozen green stem segments with a length of 10 to 15 cm; cut the stem segments into an oblique surface, leaving 2 to 3 leaves on the top; soak the stem segments in solution A for 1 hour; 3) Cutting medium and container configuration: Use a mixture of vermiculite and perlite in a container with an open top and drainage holes at the bottom. 4) Cutting operation: Insert the treated stem segment into the substrate to a depth of 1 / 3 of the stem segment length; 5) Light and humidity control: Add solution B to the ultrasonic humidifier and use atomization technology to maintain the humidity in the container; set up an LED full-spectrum plant light 10 cm above the plant to provide a lighting environment; 6) Observation and management: The environmental conditions were maintained and the growth of the plants was observed.

2. The method according to claim 1, characterized in that Step 1) The artificial environment has a light intensity of 200 μmol / m 2 ·s -1 , relative humidity 70%, day temperature 25℃, night temperature 15℃.

3. The method according to claim 1, characterized in that Step 2) The formula of solution A is: indolebutyric acid 12000ppm; naphthylacetic acid 1000ppm; sucrose 10000ppm; nano zinc 25-125ppm; nano silver 10-60ppm; nano iron oxide 15-70ppm; the rest is water.

4. The method according to claim 3, characterized in that The formula of solution A is: 12000 ppm of indolebutyric acid; 1000 ppm of naphthylacetic acid; 10000 ppm of sucrose; 75 ppm of nano zinc; 35 ppm of nano silver; 45 ppm of nano iron oxide; and the rest is water.

5. The method according to claim 1, characterized in that Step 3) The volume ratio of vermiculite to perlite is 1:

1.

6. The method according to claim 1, characterized in that Step 5) The illumination intensity in the illumination environment is 200 μmol / m 2 ·s -1 ; The formula of the B solution is 5000-10000ppm of polyethylene glycol, 500ppm of copper sulfate, 100ppm of Tween, 10-30ppm of methyl jasmonate, and the rest is water.

7. The method according to claim 6, characterized in that The formula of the B solution is 8000ppm of polyethylene glycol, 500ppm of copper sulfate, 100ppm of Tween, 20ppm of methyl jasmonate, and the rest is water.

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