A western flower thrips attracting composition and use thereof

Abstract

The application belongs to the field of plant protection, and particularly relates to a kind of western flower thrips luring composition and application. The western flower thrips luring composition is composed of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-alpha-pinene and 2,6,6-trimethyl-bicyclohept-2-ene. The above-mentioned five kinds of compounds are obtained by enriching zucchini volatiles, separating and screening volatiles, and are mixed to prepare the western flower thrips luring composition according to the volume ratio. The field test results of the western flower thrips luring composition show that the western flower thrips luring composition has significant luring activity on western flower thrips, is simple to prepare, is friendly to the environment, is non-toxic to human and livestock, and has good popularization and application prospect.

Description

Technical Field

[0001] This invention belongs to the field of plant protection, and specifically relates to a western flower thrips attractant composition and its application. Background Technology

[0002] Western flower thrips (Frankliniella occidentalis), also known as alfalfa thrips, belongs to the order Thysanoptera, family Thripidae, and genus Frankliniella. It has over 500 host plants belonging to 66 families, primarily damaging vegetable crops such as peppers, eggplants, cucumbers, lettuce, and tomatoes. Western flower thrips damage the leaves, flowers, and fruits of plants, using their rasping-sucking mouthparts to extract nutrients from plant tissues and organs to meet their own growth and development needs. They cause significant damage to crops through feeding, egg-laying, and virus transmission. Currently, the control of western flower thrips in production still relies mainly on chemical control. However, due to the small size, easy concealment, and the fact that eggs are laid inside plant tissues, control is difficult. Furthermore, the frequent use of chemical agents not only increases the resistance of western flower thrips but also pollutes the environment. Therefore, exploring pollution-free, sustainable, and green control technologies is urgently needed.

[0003] Plant-derived pesticides have become a hot topic in the research and application of new pesticides due to their safety and harmlessness to human health, environmental friendliness, and low likelihood of inducing resistance. Currently, there are a few studies on novel pesticides for the control of western flower thrips; for example, CN112544627A discloses the use of an insecticidal composition in the control of cucumber yellow-breasted thrips, rose western flower thrips, or mango tea yellow thrips. The active components of this insecticidal composition include emamectin benzoate and spirotetramat. This composition significantly enhances the activity against cucumber yellow-breasted thrips, rose western flower thrips, or mango tea yellow thrips, but its inhibitory effect on the reproductive capacity of western flower thrips is poor, and it cannot effectively kill western flower thrips in hidden areas of crops, making the infestation prone to recurrence.

[0004] Because western flower thrips are small and highly concealed, often hiding in relatively hidden environments such as flower buds and under leaves, conventional chemical pesticides have difficulty reaching these hiding places, leading to frequent infestations. Attractants developed based on insects' preferred food sources or their volatile compounds play a crucial role in their food selection behavior, and attractants that attract insects have become an important means of green pest control. Attractants work on both female and male insects, exhibiting a wide range of effects and improving pest control efficacy. Currently, some screening and applications of thrips attractants have been reported. With increased understanding of plant volatile components, compounds with thrips-attracting effects are constantly being discovered, but this is still far from sufficient. Therefore, it is necessary to identify and screen new components that can be used as western flower thrips attractants to improve the trapping effect of western flower thrips. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a western flower thrips attractant composition and its application. This invention aims to collect volatiles from zucchini, further screen these volatiles to obtain substances with attractant activity against western flower thrips, and further conduct formulation studies on the obtained substances to obtain a western flower thrips attractant composition. This composition is then applied to the preparation of western flower thrips attractants, providing a new option for the control of western flower thrips.

[0006] On one hand, the present invention provides a western flower thrips attractant composition, which is composed of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene.

[0007] Furthermore, in the western flower thrips attractant composition, the volume ratio of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclohept-2-ene is 25:20:5:15:35.

[0008] Furthermore, in the western flower thrips attractant composition, the concentration of 1,3-dimethylbenzene is 1-600 mg / L.

[0009] Furthermore, in the western flower thrips attractant composition, the concentration of p-xylene is 10-500 mg / L.

[0010] Furthermore, in the western flower thrips attractant composition, the concentration of 1,7,7-trimethyltricycloheptane is 1-300 mg / L.

[0011] Furthermore, in the western flower thrips attractant composition, the concentration of 1R-α-pinene is 10-700 mg / L.

[0012] Furthermore, in the western flower thrips attractant composition, the concentration of 2,6,6-trimethyl-bicyclohepta-2-ene is 1-400 mg / L.

[0013] Furthermore, the components 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclohept-2-ene of the thrips attractant composition are obtained by enriching zucchini volatiles, and then separating and screening the enriched zucchini volatiles.

[0014] On the other hand, the present invention provides the use of the western flower thrips attractant composition in the preparation of western flower thrips attractants.

[0015] Furthermore, the western flower thrips attractant composition is applied to western flower thrips on cucumbers, peppers, bell peppers, tomatoes, strawberries, grapes, orchids, and chrysanthemums.

[0016] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0017] The western flower thrips attractant composition provided by this invention was prepared by enriching volatiles from zucchini and separating and screening five compounds with attractant activity against western flower thrips: 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclohept-2-ene. These five compounds were then mixed to obtain the western flower thrips attractant composition. Field trapping experiments showed that the western flower thrips attractant composition provided by this invention has significant attractant activity against western flower thrips. The western flower thrips attractant composition provided by this invention is derived from natural plant components, exhibits significant attractant activity against western flower thrips, and is characterized by its green, high-efficiency, and broad-spectrum nature, providing a new option for thrips control and showing promising application prospects. Detailed Implementation

[0018] The technical solution of the present invention will be described below with reference to the embodiments. However, the present invention is not limited to the following embodiments.

[0019] To enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below with reference to specific embodiments, but the embodiments are not intended to limit the present invention.

[0020] Unless otherwise specified, the experimental and detection methods described in the following embodiments are conventional methods; the reagents and materials described are commercially available unless otherwise specified. The 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane described in this embodiment is the same as the 1,7,7-trimethyltricycloheptane described in this invention.

[0021] Example 1

[0022] This embodiment provides a method for collecting, separating, and identifying volatile substances from zucchini.

[0023] Collection of volatiles from zucchini: Dynamic headspace adsorption (DSA) was used to collect volatiles from zucchini. The zucchini was ground and added to a 20 mL headspace vial. The vial was incubated in a 60 °C water bath for 5 min. An SPME needle was inserted, and headspace extraction was performed at 60 °C for 30 min. The extracted volatiles were then analyzed using an Agilent 7890A / 5975C gas chromatography-mass spectrometry (GC-MS) system. The column program for the analysis was 40 °C for 5 min, followed by 8 °C / min. -1 The temperature was rapidly increased to 250℃ and held constant for 10 min; the run time was 41.25 min; the inlet sample was high-purity He; the flow mode was split; the heater temperature was 250℃; the pressure was 48.75 kPa; and the total flow rate was 9 mL / min. -1 The split ratio was 5:1; the column was a DB-5ms, set at 325℃, with dimensions of 30m × 250μm × 0.25μm; the low mass number was 40.0, the high mass number was 400.0, the ion source was an EI source at 230℃, and the quadrupole temperature was 150℃. The experiment was repeated 6 times, and all tested zucchini samples were collected only once.

[0024] Separation and identification of volatiles from zucchini: The collected volatiles from zucchini were analyzed using an Agilent 7890A / 5975C gas chromatography-mass spectrometry system. Based on the peaks in the total ion chromatogram, standard mass spectra were retrieved using a mass spectrometry computer data system to determine the chemical components. The relative mass fraction of each chemical component was determined using the peak area normalization method, and the chemical components and structures of the volatiles from zucchini were identified.

[0025] A total of 124 volatile compounds from zucchini were isolated and identified. Based on extensive literature review and the laboratory's previous research experience in attracting and killing thrips from western flowers, five compounds with high abundance were selected from the isolated volatile compounds as candidates for further research. The five volatile compounds obtained are shown in Table 1.

[0026] Table 1. Volatile substances from zucchini

[0027] Serial Number Element CAS number 1 1,3-Dimethylbenzene 108-38-3 2 p-xylene 106-42-3 3 1,7,7-Trimethyltricyclo[2.2.1.0(2,6)]heptane 508-32-7 4 1R-α-pinene 7785-70-8 5 2,6,6-Trimethyl-bicyclo[3.1.1]hept-2-ene 80-56-8

[0028] Example 2

[0029] The volatile compounds from zucchini obtained in Example 1 were prepared into formulations with liquid paraffin at concentrations of 0.01 wt%, 0.1 wt%, and 1 wt%, respectively. These formulations were then used to conduct behavioral regulation tests on western flower thrips, and compounds with an inducing effect on western flower thrips behavior were preliminarily screened out.

[0030] Test insects: Western flower thrips were an experimental population raised for multiple generations in a climate chamber in the inventor's laboratory using fresh green bean pods. The rearing conditions were a photoperiod of 14L:10D, relative humidity of 65% ± 5%, and temperature of (26 ± 1)℃. Western flower thrips that had emerged 3 days prior were selected for testing.

[0031] Test Method: A Y-shaped olfactory instrument was used for testing. The Y-shaped olfactory instrument was made of transparent glass, with a base length of 16cm, two arms lengths of 15cm, an inner diameter of 2cm, and an angle of 60° between the arms. For behavioral testing of adult western flower thrips, one arm of the Y-shaped olfactory instrument was connected to a odor source bottle containing volatile substances from zucchini, and the other arm was connected to a odor source bottle containing liquid paraffin as a control. Aeration was carried out for 5 minutes to fill the tube with odor. The airflow rate of the two arms of the Y-shaped tube was controlled at 200mL / min, the indoor temperature was controlled at (25±1)℃, and the relative humidity was 60%~80%. Before the experiment, the tested adult western flower thrips were starved for 5 hours, and each insect was tested only once. One test insect was introduced into the base of the glass tube of a Y-shaped olfactory instrument. The test lasted 5 minutes. If the insect crawled beyond one-third of the length of a test tube arm and remained there for more than 30 seconds, it was considered that the insect had selected that arm, indicating that the compound had an attractive effect on western flower thrips; otherwise, it was recorded as no selection. Five insects were tested per treatment, repeated three times. After each treatment, the Y-shaped olfactory instrument and other test materials were rinsed with anhydrous ethanol. The glass parts were then placed in a 180°C electric thermostatic drying oven for 120 minutes, while the plastic and rubber parts were placed in an 80°C electric thermostatic drying oven for 60 minutes. Liquid paraffin was used as a control.

[0032] The results of the behavioral test of western flower thrips are shown in Table 2. The mass spectra of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene showed that five compounds had an attraction effect on western flower thrips.

[0033] Table 2 Results of behavioral tests on western flower thrips

[0034]

[0035] Example 3

[0036] This example demonstrates a behavioral test of five different concentrations of zucchini volatiles on western flower thrips.

[0037] The 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene used in this embodiment were all purchased from Beijing Bailingwei Technology Co., Ltd., and their purity was ≥95%.

[0038] A gradient solution of the above five volatile compounds was prepared using liquid paraffin as a solvent:

[0039] 1,3-Dimethylbenzene: (0.1, 1, 10, 100, 200, 400, 600, 800, 1000 mg / L);

[0040] p-Xylene: (1, 10, 100, 200, 300, 400, 500, 600, 700 mg / L);

[0041] 1,7,7-Trimethyltricyclo[2.2.1.0(2,6)]heptane: (0.1, 1, 10, 100, 200, 300, 400, 500 mg / L);

[0042] 1R-α-pinene: (1, 10, 100, 200, 400, 500, 600, 700, 800, 900 mg / L);

[0043] 2,6,6-Trimethyl-bicyclo[3.1.1]hept-2-ene: (0.1, 1, 10, 100, 200, 300, 400, 500, 600 mg / L).

[0044] Measurement Method: A Y-shaped olfactory instrument was used for testing. The Y-shaped olfactory instrument was made of transparent glass, with a base length of 16 cm, two arms lengths of 15 cm, an inner diameter of 2 cm, and an angle of 60° between the two arms. For behavioral testing of adult western flower thrips, one arm of the Y-shaped olfactory instrument was connected to a odorant bottle containing volatile substances from zucchini, and the other arm was connected to a odorant bottle containing liquid paraffin as a control. Aeration was carried out for 5 minutes to fill the tube with odor. The airflow rate of the two arms of the Y-shaped tube was controlled at 200 mL / min, the indoor temperature was controlled at (25±1)℃, and the relative humidity was 60%–80%. Before the experiment, the tested adult western flower thrips were starved for 5 hours, and each insect was tested only once. One test insect was introduced into the base of the glass tube of a Y-shaped olfactory instrument. The test lasted 5 minutes. If the insect crawled beyond one-third of the length of a test tube arm and remained there for more than 30 seconds, it was considered that the insect had selected that arm, indicating that the compound had an attractive effect on western flower thrips; otherwise, it was recorded as no selection. Five insects were tested per treatment, repeated three times. After each treatment, the Y-shaped olfactory instrument and other test materials were rinsed with anhydrous ethanol. The glass parts were then placed in a 180°C electric thermostatic drying oven for 120 minutes, while the plastic and rubber parts were placed in an 80°C electric thermostatic drying oven for 60 minutes. Liquid paraffin was used as a control.

[0045] The results of the Y-type olfactometer measurements are shown in Table 3. The results show that 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene have significant attraction activities on the behavior of western flower thrips. Among them, the concentrations of 1,3-dimethylbenzene (1-600 mg / L), p-xylene (10-500 mg / L), 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane (1-300 mg / L), 1R-α-pinene (10-700 mg / L), and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene (1-400 mg / L) have significant attraction activities on the behavior of western flower thrips.

[0046] Table 3. Behavioral test results of five different concentrations of zucchini volatiles on western flower thrips.

[0047]

[0048]

[0049] The test results are shown in Table 3. Treatments with 3 or fewer insects were considered to have no attraction effect on western flower thrips. Concentrations of 1,3-dimethylbenzene (1-600 mg / L), p-xylene (10-500 mg / L), 1,7,7-trimethyltricycloheptane (1-300 mg / L), 1R-α-pinene (10-700 mg / L), and 2,6,6-trimethyl-bicyclohept-2-ene (1-400 mg / L) all had an attraction effect on western flower thrips.

[0050] Example 4

[0051] This embodiment provides the formulation of a western flower thrips attractant composition.

[0052] The following components, which exhibited significant attraction activity against western flower thrips behavior in Example 3: 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene. Different concentrations of these components were prepared to create different western flower thrips attractant compositions. The composition was formulated according to a volume ratio of 1,3-dimethylbenzene: p-xylene: 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane: 1R-α-pinene: 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene = 25:20:5:15:35.

[0053] #1 Thrips Attractant Composition: 10 mg / L of 1,3-dimethylbenzene, 10 mg / L of p-xylene, 1 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 10 mg / L of 1R-α-pinene, and 1 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #1 thrips attractant composition for later use.

[0054] #2 Western Flower Thrips Attractant Composition: 100 mg / L of 1,3-dimethylbenzene, 50 mg / L of p-xylene, 10 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 50 mg / L of 1R-α-pinene, and 5 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #2 Western Flower Thrips Attractant Composition for later use.

[0055] #3 Thrips Attractant Composition for Western Flower: 600 mg / L of 1,3-dimethylbenzene, 100 mg / L of p-xylene, 100 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 100 mg / L of 1R-α-pinene, and 10 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #3 Thrips Attractant Composition for Western Flower.

[0056] #4 Western Flower Thrips Attractant Composition: 100 mg / L of 1,3-dimethylbenzene, 10 mg / L of p-xylene, 200 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 200 mg / L of 1R-α-pinene, and 100 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #4 Western Flower Thrips Attractant Composition for later use.

[0057] #5 Thrips Attractant Composition: 200 mg / L of 1,3-dimethylbenzene, 200 mg / L of p-xylene, 50 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 400 mg / L of 1R-α-pinene, and 200 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #5 Thrips Attractant Composition for use.

[0058] #6 Thrips Attractant Composition: 500 mg / L of 1,3-dimethylbenzene, 500 mg / L of p-xylene, 300 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 500 mg / L of 1R-α-pinene, and 300 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #6 Thrips Attractant Composition for use.

[0059] #7 Western Flower Thrips Attractant Composition: 600 mg / L of 1,3-dimethylbenzene, 100 mg / L of p-xylene, 300 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 700 mg / L of 1R-α-pinene, and 400 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #7 Western Flower Thrips Attractant Composition for later use.

[0060] #8 Thrips Attractant Composition: 600 mg / L of 1,3-dimethylbenzene, 300 mg / L of p-xylene, 100 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 400 mg / L of 1R-α-pinene, and 200 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene were mixed in a volume ratio of 25:20:5:15:35 to prepare the #8 Thrips Attractant Composition for use.

[0061] Example 5

[0062] This example is a field trapping experiment of western flower thrips.

[0063] Using a pipette, 200 μL of the western flower thrips attractant composition was added dropwise onto a septum of a rubber stopper that had been cleaned with n-hexane. After the n-hexane evaporated, 1 mL of dichloromethane was added dropwise in portions. After the dichloromethane evaporated, the western flower thrips attractant lure was obtained. Each lure contained the western flower thrips attractant composition. The prepared lures were placed in aluminum foil, sealed, and stored at -20°C.

[0064] In the field trapping experiment, the lure of the western flower thrips attractant composition was fixed to sticky traps. The control (CK) was the treatment without the western flower thrips attractant composition lure; groups #1 to #8 were treatments with the western flower thrips attractant composition lure. Each treatment had three replicates, randomly arranged, with adjacent sticky traps spaced 15–30 m apart. Starting from the second day after setup, the number of western flower thrips trapped was recorded every morning and promptly removed, with the observation continuing for 30 days.

[0065] Table 4 Results of field trapping trials using western flower thrips attractant combinations

[0066]

[0067] The experimental results are shown in Table 4. All #1-#8 thrips attractant compositions showed attraction activity to western flower thrips, and were superior to the control group, indicating that the thrips attractant compositions provided by this invention have attraction activity to western flower thrips. Sex identification of the captured insects revealed that the majority were female western flower thrips, with a minority being male, indicating that the thrips attractant compositions provided by this invention have attraction activity to both male and female western flower thrips. Among them, #6, #7, and #8 thrips attractant compositions showed even higher attraction activity to western flower thrips.

[0068] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A western flower thrips attractant composition, characterized in that, The western flower thrips attractant composition consists of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene.

2. The western flower thrips attractant composition according to claim 1, characterized in that, The western flower thrips attractant composition was prepared by mixing 500 mg / L of 1,3-dimethylbenzene, 500 mg / L of p-xylene, 300 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 500 mg / L of 1R-α-pinene and 300 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene in a volume ratio of 25:20:5:15:

35.

3. The western flower thrips attractant composition according to claim 1, characterized in that, The western flower thrips attractant composition was prepared by mixing 600 mg / L of 1,3-dimethylbenzene, 100 mg / L of p-xylene, 300 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 700 mg / L of 1R-α-pinene, and 400 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene in a volume ratio of 25:20:5:15:

35.

4. The western flower thrips attractant composition according to claim 1, characterized in that, The western flower thrips attractant composition was prepared by mixing 600 mg / L of 1,3-dimethylbenzene, 300 mg / L of p-xylene, 100 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 400 mg / L of 1R-α-pinene, and 200 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene in a volume ratio of 25:20:5:15:

35.

5. The western flower thrips attractant composition according to claim 1, characterized in that, The components of the thrips attractant composition, 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene, were obtained by enriching and separating volatiles from zucchini.

6. The use of the western flower thrips attractant composition according to any one of claims 1-5 in the preparation of western flower thrips attractants.

7. The application according to claim 6, characterized in that, The western flower thrips referred to are those found on cucumbers, peppers, bell peppers, tomatoes, strawberries, grapes, orchids, and chrysanthemums.

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