Composition containing flumetralin and butralin and used for tobacco plant growth regulation and application thereof

Abstract

The invention relates to a pesticide composition containing flumetralin and butralin and application of the pesticide composition. The composition is used for inhibiting the growth of tobacco axillary buds and can effectively improve the tobacco yield and reduce the manual topping cost. The product has the advantages of high stability, simple preparation in the processing process, no dust generation in the production process, and effective increase of the utilization rate and reduction of the usage amount by mainly selecting the environment-friendly organic solvent, thereby reducing the residual amount in the environment. The composition has the advantages of low acute toxicity, acceptable dietary risk and safety to human and livestock, meets the actual requirements of current agricultural production, has the inhibition rate on tobacco axillary buds obviously higher than that of butralin and flumetralin, and through compounding, the dosage of a single tobacco plant is obviously reduced, and pesticide residues are reduced. And compared with similar products, the cost is lower, and the use cost of the pesticide by a user is reduced. Popularization and use of the method can enrich the pesticide market, and farmers have more choices.

Description

Technical Field

[0001] The invention relates to the field of agricultural crop growth control, and relates to a plant growth regulator composition containing butralin, fluazifop and related adjuvants, which is applied to chemical topping of tobacco. Background Art

[0002] Flumetralin is a contact and local systemic plant growth regulator that mainly affects the function of the enzyme system in the plant, increases the chlorophyll and protein content, and inhibits the growth of tobacco lateral buds.

[0003] Butyl, also known as sprout-stopping agent, is a contact and local systemic sprout inhibitor. Its mechanism of action is to inhibit the cell division of meristem, thereby inhibiting the growth of young shoots and roots of weeds.

[0004] After the tobacco plant is toppled, the dormant axillary buds germinate rapidly from top to bottom, consuming a large amount of water and nutrients from the leaves, thereby reducing the quality and yield of tobacco leaves. In tobacco production, manual topping and shaving are often performed after the tobacco plants have budded to promote the growth of tobacco leaves and improve the quality and characteristics of tobacco leaves. The correct and reasonable use of bud inhibitors can not only reduce labor consumption, but also reduce the spread and repeated infection of diseases caused by agricultural operations.

[0005] Both flumetralin and butralin are highly effective tobacco axillary bud inhibitors. Flumetralin is a contact and local systemic tobacco lateral bud inhibitor that can be absorbed through the stems and leaf surfaces of tobacco, inhibiting the division and growth of cells in the axillary buds, thereby inhibiting the germination of axillary buds. Applying the drug once when topping tobacco can inhibit the growth of axillary buds throughout the season, and at the same time significantly increase tobacco leaf yield and quality. Butralin is a contact plant growth regulator that mainly inhibits cell division, causing axillary buds within 2.5 leaves to stop growing and curl and wilt. Ungerminated axillary buds cannot grow out. Applying the drug once can inhibit the occurrence of tobacco axillary buds until the end of harvest, saving a lot of labor for removing axillary buds. Summary of the invention

[0006] The invention develops a composition for regulating the growth of tobacco plants, wherein the mass ratio of flumetralin to butralin in the composition is 12.5:18.0, and the dosage form of the composition is emulsifiable concentrate.

[0007] The composition for tobacco plant growth regulator of the present invention comprises 18.0% of butralin, 12.5% ​​of flumetralin, 10% of dimethyl sulfoxide, 7% of cyclohexanone, 8% of xylene, 5.5% of phenethylphenol polyoxyethylene ether, 4.5% of calcium dodecylbenzene sulfonate and 100% of 150# solvent oil.

[0008] Butyl is a contact plant growth regulator that mainly inhibits cell division, thereby causing the axillary buds to wilt. Flumetralin is a contact and local systemic tobacco lateral bud inhibitor that can be absorbed through the stem and leaf surface of tobacco, inhibiting the division and growth of cells in the axillary buds, thereby inhibiting the germination of axillary buds. The combination is used to inhibit the growth of tobacco axillary buds, which can effectively increase tobacco yields and reduce the cost of manual topping.

[0009] The present invention has an additive or synergistic effect on inhibiting the growth of tobacco axillary buds after the mixture of flumetralin and butamol has no antagonistic effect. It shows that the mixture of the two is reasonable for inhibiting the growth of tobacco axillary buds. According to the isobolite principle, when the mass ratio of flumetralin to butamol is 1: (1.3152-1.8346), the regulating effect on the number of tobacco axillary buds can theoretically reach the maximum synergistic effect; when the mass ratio of flumetralin to butamol is 1: (1.4267-1.6836), the regulating effect on the fresh weight of tobacco axillary buds can theoretically reach the maximum synergistic effect. Taking into account the synergistic effect on the regulating effect on the number of live buds and fresh weight of tobacco axillary buds, the study believes that when flumetralin and butamol are mixed at a mass ratio of 1: (1.4267-1.6836), the synergistic effect of inhibiting tobacco axillary buds is most obvious, which is the best ratio of the two agents mixed to inhibit the growth of tobacco axillary buds. A suitable ratio can be selected within this ratio range for production. The mass ratio of flumetralin to butralin in the present invention is 1:1.44, and the mixing is reasonable.

[0010] The results of the field efficacy test of the present invention show that the compound product has a significant inhibitory effect on the number and fresh weight of tobacco axillary buds, and the mixing can reduce the amount of the agent used, and reduce the impact on the environment and beneficial organisms. When the dosage of the active ingredient of the present invention is 20.33-22.18 mg / plant, the number and fresh weight of tobacco axillary buds are significantly inhibited, and the effect is not significantly different from the single-dose control or is significantly better than the single-dose control. The mixing of the two active ingredients can reduce the amount of the active ingredients used, reduce the pesticide residue, ensure the quality of agricultural products, and at the same time reduce the negative effects of pesticides on environmental pollution, and correspondingly reduce the harm to beneficial organisms. DETAILED DESCRIPTION

[0011] The butralin agent, flumetralin agent and the compound agent of butralin and flumetralin in the following examples were prepared by Zhangye Dagong Agrochemical Co., Ltd.

[0012] The relevant effect data in the embodiment are calculated according to the following formula.

[0013] Bud inhibition rate (%) = (number of axillary buds in the control area without bud removal - number of axillary buds in the area treated with the drug) / number of axillary buds in the control area without bud removal × 100.

[0014] Bud inhibition effect (%) = (fresh weight of axillary buds in the control area without bud removal - fresh weight of axillary buds in the area treated with the agent) / fresh weight of axillary buds in the control area without bud removal × 100.

[0015] Yield increase rate (%) = (yield of the area treated with chemical agents - yield of the area treated with artificial bud removal) / yield of the area treated with artificial bud removal × 100. Example

[0016] Experimental location: Xiajiangkou Village, Longquan Town, Yimen County, Yuxi City, Yunnan Province. Experimental crop: tobacco (variety: Liaoyan No. 20). The soil of the experimental field is yellow loam, pH value 6.0, organic matter content 2.52%. Before planting, deep plowing 20cm, trenching and ridges are about 1m wide and 25cm high. Transplanting row spacing is 120c, plant spacing is 55cm, and planting amount: about 1000 plants / mu. The experimental site is flat, with good drainage and irrigation conditions. The cultivation and water and fertilizer management conditions of all experimental plots are consistent.

[0017] Application period: after topping the tobacco plant at the beginning of flowering period.

[0018] Application method: cup spraying.

[0019] Accurately measure a certain amount of the test substance and control agent into a special bottle, mark it and bring it to the test site. Prepare the control agent solution by the secondary dilution method before applying the agent. Apply an equal amount of clean water to the clean water control. Cup the treatment area evenly from low concentration to high concentration one by one, without heavy or missing. Wash the application equipment with clean water before applying different agents.

[0020] In the artificial bud removal treatment area, axillary buds were removed once every 7 days. Three points were selected in each plot, and 10 tobacco plants were selected in each point for fixed-strain investigation. The number of axillary buds over 2 cm was investigated. In the last investigation, the axillary buds were taken and weighed for fresh weight.

[0021] 1) Influence on germination inhibition rate and germination inhibition effect The statistical results of the bud inhibition rate showed that 15 days after the application of the pesticide, no axillary buds grew after the artificial bud removal treatment. The agent of the present invention has a good effect on inhibiting the axillary buds of tobacco. When the preparation was diluted 130 times, 120 times, 110 times and 60 times, the bud inhibition rates of tobacco at 15 days were 83.2%, 91.5%, 91.7% and 94.3%, respectively; a good inhibition effect was still achieved at 29 days, with inhibition rates of 80.2%, 89.1%, 90.5% and 93.1%, respectively; the bud inhibition rates at 40 days were 75.2%, 87.4%, 87.5% and 90.6%, respectively, all of which were above 75%.

[0022]

[0023] The results of the investigation on the bud inhibition effect showed that the bud inhibition effect of the agent of the present invention on tobacco axillary buds was 80.9%, 87.4%, 88.1% and 94.3% respectively when the preparation was diluted 130 times, 120 times, 110 times and 60 times.

[0024]

[0025] 2) Impact on production The yield measurement results showed that the yield per mu of the agent of the present invention was 148.60, 160.50, 160.60 and 163.60 kg respectively when the preparation was diluted 130 times, 120 times, 110 times and 60 times, which were 23.0%, 32.9%, 32.9% and 35.4% higher than those of the blank control.

[0026]

[0027] 3) Security The present invention is safe for tobacco plants when the dosage of the preparation is 130 to 60 times the liquid, that is, the dosage of the active ingredient is 18.77 mg / plant to 40.66 mg / plant. Three days and seven days after the application of the pesticide, the tobacco in each plot within the test dosage range showed no phytotoxicity symptoms, the tobacco grew normally, and no adverse effects were observed on adjacent crops. It is safe for adjacent crops and other non-target organisms.

[0028] Example 2 Experimental location: Hetaoping Village, Qingtaiping Town, Badong County, Enshi Prefecture, Hubei Province. Test crop: tobacco, variety "Yunyan 87". The soil of the test site is sandy loam, pH value is 6.8, organic matter content is 1.6%, and soil fertility is medium. Single ridge and single row, ridge spacing is 1.2m, plant spacing is 0.55m. Planting density is 1040 plants / mu. The test site is flat, with good drainage and irrigation conditions. The cultivation and water and fertilizer management conditions of all test plots are consistent.

[0029] Application period: after topping the tobacco during the initial flowering period; Application method: cup spray; Accurately measure a certain amount of the test substance and control agent into a special bottle, mark it and bring it to the test site. Prepare the control agent solution by the secondary dilution method before applying the agent. Apply an equal amount of clean water to the clean water control. Cup the treatment area evenly from low concentration to high concentration one by one, without heavy or missing. Wash the application equipment with clean water before applying different agents.

[0030] In the artificial bud removal treatment area, axillary buds were removed once every 7 days. Three points were selected in each plot, and 10 tobacco plants were selected at each point for fixed-strain investigation. The number of axillary buds over 2 cm was investigated, and the axillary buds were taken and weighed for fresh weight at the last investigation.

[0031] 1) Influence on germination inhibition rate and germination inhibition effect The statistical results of bud inhibition rate showed that 15 days after application, no axillary buds grew after artificial bud removal treatment, and the agent of the present invention had a good effect on inhibiting tobacco axillary buds. At dosages of 18.77, 20.33, 22.18, and 40.66 mg / plant, the bud inhibition rates of tobacco at 15 days were 87.42%, 93.52%, 93.91%, and 94.1%, respectively; a good inhibition effect was still achieved at 29 days, with inhibition rates of 81.58%, 85.69%, 89.78%, and 89.78%, respectively; the bud inhibition rates at 40 days were 75.52%, 84.59%, 82.08%, and 86.72%, respectively, all of which were above 75%.

[0032]

[0033] The results of the investigation on the bud inhibition effect showed that the bud inhibition effect of the agent of the present invention on tobacco axillary buds was 82.72%, 87.20%, 88.27% and 93.07% respectively at dosages of 18.77, 20.33, 22.18 and 40.66 mg / plant.

[0034]

[0035] 2) Impact on production The yield measurement results showed that the per mu yield of the agent of the present invention at dosages of 18.77, 20.33, 22.18 and 40.66 mg / plant was 164.07, 176.6, 181.33 and 163.60 kg, respectively, which were 15.78%, 24.60%, 27.11% and 25.99% higher than those of the blank control.

[0036]

[0037] 3) Security During the entire test process of the present invention, within the test dosage range, all the tested agents had no adverse effects on the growth of tobacco leaves, and no adverse effects on the surrounding environment and other beneficial organisms in the test area were found.

[0038] Example 3 Experimental location: No. 600, Shenliao Road, Tiexi District, Shenyang, Shenyang Sinochem Pesticide Chemical Research and Development Co., Ltd. Pesticide Bioassay Center Experimental site. Experimental crop: Ruicaotang tobacco. Soil type is sandy loam with medium soil fertility. Plant spacing is 55 cm, row spacing is 85 cm. Planting quantity: about 1,000 plants / mu. The experimental site is flat with good drainage and irrigation conditions. The cultivation and water and fertilizer management conditions of all experimental plots are the same.

[0039] Application period: after topping the tobacco during the initial flowering period; Application method: cup spray; Accurately measure a certain amount of the test substance and control agent into a special bottle, mark it and bring it to the test site. Prepare the control agent solution by the secondary dilution method before applying the agent. Apply an equal amount of clean water to the clean water control. Cup the treatment area evenly from low concentration to high concentration one by one, without heavy or missing. Wash the application equipment with clean water before applying different agents.

[0040] In the artificial bud removal treatment area, axillary buds were removed once every 7 days. Three points were selected in each plot, and 10 tobacco plants were selected at each point for fixed-strain investigation. The number of axillary buds over 2 cm was investigated, and the axillary buds were taken and weighed for fresh weight at the last investigation.

[0041] 1) Influence on germination inhibition rate and germination inhibition effect The statistical results of bud inhibition rate showed that 15 days after application, no axillary buds grew after artificial bud removal treatment, and the agent of the present invention had a good effect on inhibiting tobacco axillary buds. When the preparation was diluted 130 times, 120 times, 110 times and 60 times, the bud inhibition rates of tobacco at 15 days were 85.3%, 89.4%, 94.2% and 97.4%, respectively; a good inhibition effect was still achieved at 29 days, with inhibition rates of 84.0%, 87.6%, 92.0% and 94.9%, respectively; the bud inhibition rates at 40 days were 80.4%, 84.7%, 90.5% and 92.3%, respectively, all of which were above 75%.

[0042]

[0043] The results of the investigation on the bud inhibition effect showed that the bud inhibition effect of the agent of the present invention on tobacco axillary buds was 83.1%, 86.1%, 92.0% and 94.1% respectively when the preparation was diluted 130 times, 120 times, 110 times and 60 times.

[0044]

[0045] 2) Impact on production The yield measurement results showed that the per mu yield of the agent of the present invention was 179.8, 184.5, 191.9 and 192.1 kg respectively when the preparation was diluted 130 times, 120 times, 110 times and 60 times, which were 14.4%, 17.5%, 22.1% and 22.9% higher than those of the blank control.

[0046]

[0047] 3) Security The present invention is safe for tobacco plants when the dosage of the preparation is 130 to 60 times the liquid, that is, the dosage of the active ingredient is 18.77 mg / plant to 40.66 mg / plant. The growth, plant height and leaf color of tobacco in each treatment area of ​​the test agent within the test dosage range were observed 3 days and 7 days after the application of the drug. No symptoms of drug damage were found, which was better than the blank control area.

[0048] The preferred embodiments of the present invention are described in detail above, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A composition containing flumetralin and butralin for regulating tobacco plant growth, wherein the mass ratio of flumetralin to butralin in the composition is 12.5:18.

0. The composition is in the form of emulsifiable concentrate.

2. The tobacco plant growth regulator composition according to claim 1, characterized in that The total weight of flumetralin and butralin accounts for 30.5% of the weight of the composition.

3. The tobacco plant growth regulator composition according to claim 1, wherein the composition comprises 18.0% of butralin, 12.5% ​​of flumetralin, 10% of dimethyl sulfoxide, 7% of cyclohexanone, 8% of xylene, 5.5% of phenethylphenol polyoxyethylene ether, 4.5% of calcium dodecylbenzene sulfonate, and 150# solvent oil (complementing 100%).

4. The composition according to claims 1-3 is characterized in that it is used to inhibit the growth of tobacco axillary buds, the dosage is 18.78-22.18 mg / plant, and the method of use is cup spraying.

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