Bactericide composition for preventing and treating grape powdery mildew

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating grape powdery mildew. The invention relates to a bactericide composition for preventing and treating grape powdery mildew. The effective components of the bactericide composition are compounded by penflufen and mandipropamid or cnidium lactone. The penflufen is compounded with mandipropamid or cnidium lactone, and a pot experiment shows that the penflufen and mandipropamid are compounded in a compounding mass ratio range of (1-7): (25-1), so that the penflufen and mandipropamid have a synergistic effect on grape powdery mildew; the penflufen and the cnidium lactone are compounded in a compounding mass ratio range of (1-19): (19-1), so that the penflufen and the cnidium lactone have a synergistic effect on grape powdery mildew. Therefore, when the penflufen is compounded with mandipropamid or cnidium lactone, the prevention and treatment effect on the grape powdery mildew can be improved, the dosage of the medicament can be reduced, meanwhile, the generation of drug resistance can be delayed, and a support can be provided for developing a novel medicament for preventing and treating the grape powdery mildew.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a fungicide composition for preventing and controlling grape powdery mildew. Background Art

[0002] Grape powdery mildew is a fungal disease caused by the obligate parasite Uncinula necater, which can infect green tissues such as grape leaves, new shoots and bunches. When leaves are affected, chlorotic spots appear on the front, the veins on the back turn black, and a powdery mildew layer forms on both sides of the spots. In severe cases, the powdery mildew layer extends to the entire leaf surface, causing the leaves to dry and curl. When the bunches are affected, chlorotic irregular gray-white spots appear on the surface, which later develop into yellow-brown spots covered with white powder. When young fruits are affected, chlorotic radial lines appear on the surface, the epidermal cells necrotize, and white powder appears. The young fruits grow slowly and are deformed, and have no edible value.

[0003] In the production process, the application of fungicides such as thiophanate-methyl, tebuconazole, hexaconazole, thiophanate-methyl and methyl thiophanate can prevent and control grape powdery mildew. However, when using fungicides to prevent and control diseases, the problem of drug resistance cannot be ignored. Long-term use of a single fungicide is likely to cause the rapid accumulation and development of drug resistance in pathogens, resulting in reduced prevention effectiveness. According to relevant reports, grape powdery mildew fungi have developed varying degrees of resistance to a variety of existing fungicides, so it is necessary to study new agents for the prevention and control of grape powdery mildew. Summary of the invention

[0004] The object of the present invention is to provide a fungicide composition for preventing and controlling grape powdery mildew, so as to solve the problem that grape powdery mildew fungi have developed different degrees of resistance to various existing fungicides.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A fungicide composition for preventing and controlling grape powdery mildew, wherein the active ingredient is a compound of pentopyralid and dimethomorph or osthole.

[0007] More specifically, the mass ratio of penpyraclostrobin to mandipyridam is 1-7:25-1.

[0008] More specifically, the mass ratio of pentozolin to osthole is 1-19:19-1.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] The present invention compounds pentocyclan with dimethomorph or osthole, and through potted plant experiments, it is found that within the compounding mass ratio range of 1-7:25-1, the compounding of pentocyclan and dimethomorph shows a synergistic effect on grape powdery mildew; within the compounding mass ratio range of 1-19:19-1, the compounding of pentocyclan and osthole shows a synergistic effect on grape powdery mildew. It is explained that when pentocyclan is compounded with dimethomorph or osthole, the control effect on grape powdery mildew can be improved, which is helpful to reduce the dosage of the agent, and at the same time, the generation of drug resistance can be delayed, which can provide support for the development of new agents for controlling grape powdery mildew. DETAILED DESCRIPTION

[0011] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0012] Example: Indoor toxicity assay

[0013] 1. Test crops

[0014] One-year-old Kyoho grape seedlings, one plant per pot, numbered and kept for later use.

[0015] 2. Test spore suspension

[0016] Grape powdery mildew fungus Uncinula necater was inoculated on the test crop. After fresh spores were produced, the powdery mildew spores on the back of the leaves were washed with sterile water and filtered through double gauze to adjust the spore concentration to 5.0×10 6 spores / mL for later use.

[0017] 3. Test reagents

[0018] 96% trifloxystrobin technical (Inner Mongolia Zhonggao Chemical Co., Ltd.), 93% dimethomorph technical (Syngenta Crop Protection Co., Ltd.), 10% osthole technical (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.)

[0019] The original drug was first dissolved in dimethyl sulfoxide and then diluted with 0.05% Tween-80 aqueous solution to form a single-dose mother solution; the cnidium monnieri mother drug was directly diluted with 0.05% Tween-80 aqueous solution to form a single-dose mother solution. Multiple groups of proportions were set, and each single dose and proportioned mixture was set with 5 mass concentration gradients according to the equal ratio method.

[0020] 4. Test methods

[0021] The spore suspension was sprayed evenly on the leaves of the test crop. After 24 hours, the prepared liquid was sprayed evenly. Each mass concentration of liquid was repeated 5 times, and 3 pots of test crops were sprayed in each repetition. The treatment of spraying an equal amount of 0.05% Tween-80 aqueous solution was used as a blank control. After the treatment, the potted plants were transferred to 25°C, 90% relative humidity, and 12L / 12D light conditions for cultivation. When spraying spores and spraying liquid, the front and back of the leaves were wet and the liquid did not drip.

[0022] After the blank control is fully infected, the disease condition is graded and investigated. The disease grading method is shown in Table 1. The disease index is calculated and the prevention effect is calculated using the disease index.

[0023] Table 1 Disease classification method

[0024]

[0025] The disease index was calculated according to the following formula:

[0026]

[0027] The disease prevention effect is calculated according to the following formula:

[0028]

[0029] 5. Data Analysis

[0030] The logarithmic value of the pesticide concentration (x) was used as the independent variable, and the probability value of the control effect (y) was used as the dependent variable to calculate the toxicity regression equation, EC 50 The synergistic effect of the mixed agent was evaluated based on the synergistic coefficient SR and the correlation coefficient. The results are shown in Table 2-3.

[0031] Table 2 The combined effect of fluopicolide and dimethomorph on grape powdery mildew

[0032]

[0033] Table 3 The combined effect of fluopicolide and osthole on grape powdery mildew

[0034]

[0035] It can be seen from the test results in Table 2 and Table 3 that within the range of the compound mass ratio of 1-7:25-1, the combination of pentocyclam and dimethomorph has a synergistic effect on grape powdery mildew; within the range of the compound mass ratio of 1-19:19-1, the combination of pentocyclam and osthole has a synergistic effect on grape powdery mildew.

[0036] In summary, when the pentafluoroacetic acid anilide of the present invention is compounded with dimethomorph or osthole, the control effect on grape powdery mildew can be improved, which helps to reduce the dosage of the agent and delays the development of drug resistance, thereby providing support for the development of new agents for controlling grape powdery mildew.

[0037] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A fungicide composition for preventing and controlling grape powdery mildew, characterized in that: The active ingredient is a combination of pentocyclanil and dimethomorph or osthole.

2. The fungicide composition for preventing and controlling grape powdery mildew according to claim 1, characterized in that: The mass ratio of penpyraclostrobin to mandipyridam is 1-7:25-1.

3. The fungicide composition for preventing and controlling grape powdery mildew according to claim 1, characterized in that: The mass ratio of fluopicolide to osthole is 1-19:19-1.

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