Combined bactericide and application thereof in preventing and treating rice bacterial streak

Abstract

The invention relates to a combined bactericide and application thereof in preventing and treating rice bacterial streak, and belongs to the technical field of pesticide compounding. The combined bactericide comprises nano zinc and copper oxychloride in a mass ratio of (3-15): (15-35), and further comprises a pesticide adjuvant and a solvent, the nano zinc is zinc oxide powder with the particle size of less than 100nm; the copper oxychloride is powder with the fineness of 2-5 microns. The combined bactericide can be used for preventing and treating rice bacterial diseases, and the rice bacterial diseases are rice bacterial streak. The nano zinc and the copper oxychloride are broad-spectrum fungicides and are wide in application range, the compounded composition has a synergistic effect, the nano zinc has protection and treatment effects on plants, the disease resistance of the plants can be enhanced, the tolerance of the plants can be improved, the control effect can be further improved through the characteristic, and the effect is obviously higher than that of a single agent of the copper oxychloride, so that the composition is worthy of popularization and application. And the composition can be well mixed with other chemical pesticides.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and particularly relates to a combined fungicide and application thereof in preventing and controlling rice bacterial leaf streak disease. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Crop pest control is one of the important links in agricultural production and has always been a key topic of concern for agricultural science and technology workers. In the process of chemical control of harmful organisms, due to the increase in the use of pesticides and the influence of factors such as unscientific use of pesticides, farmers' pesticide costs have increased, and pesticide residues in agricultural products are prone to exceed the standard, which in turn causes a series of problems such as environmental pollution.

[0004] Bacterial leaf streak disease of rice is caused by the pathogenic variety of Xanthomonas oryzae, which can lead to a yield reduction of 10% to 20%. In severe cases, the reduction can reach more than 40%, or even cause a total rice crop failure.

[0005] Nano zinc is an inorganic metal nanoparticle. Its active ingredient is nano-scale zinc oxide naturally generated on the surface of nanoparticles. It exerts its antibacterial mechanism by releasing zinc ions in the microenvironment.

[0006] Copper oxychloride, also known as copper oxychloride and basic copper chloride, is a low-toxic inorganic copper protective fungicide. Its mechanism of action is that copper oxychloride forms a film on the surface of crops, which releases copper ions under a certain humidity. Copper ions can destroy the cell membrane of pathogens, making them inactive, thereby preventing pathogens from invading plants. However, the upper limit of the protective effect of copper oxychloride alone on rice bacterial leaf streak is about 80%, which cannot achieve a good control effect. Summary of the invention

[0007] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a combined fungicide and its application in preventing and controlling rice bacterial leaf streak disease. The combination of nano zinc and copper oxychloride effectively reduces the dosage and cost of the drug, achieves better prevention and control effects, and reduces the adverse impact on the environment.

[0008] In order to achieve the above object, the technical solution of the present invention is:

[0009] In a first aspect, a combined fungicide comprises: nano zinc and copper oxychloride in a mass ratio of (3-15):(15-35), and also comprises a pesticide adjuvant and a solvent;

[0010] The nano zinc is zinc oxide powder with a particle size less than 100 nm; the copper oxychloride is powder with a fineness of 2 to 5 μm.

[0011] Optionally, the pesticide adjuvant includes one or more of alkylnaphthalene formaldehyde condensate sulfonate, nonylphenol polyoxyethylene ether, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether, EO-PO block polyether, sodium dodecyl sulfonate, triphenyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether, polyoxyethylene ether sulfate, xanthan gum, methyl ethoxy silicone oil defoamer, magnesium aluminum silicate and urea, and the solvent includes one or more of water, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, capric amide, methyl oleate and rosin-based vegetable oil.

[0012] Optionally, the dosage form of the combined fungicide is one of a solution, an aqueous emulsion, a microemulsion and a suspension.

[0013] Optionally, the mass percentage of the nano zinc and copper oxychloride in the combined fungicide is (3-15) %:

[0014] (15~35)%.

[0015] In a second aspect, a use of the above-mentioned combined fungicide in preventing and controlling rice bacterial leaf streak disease.

[0016] Optionally, in the application, the fungicide combination is diluted 500 to 800 times and then sprayed on the rice leaves at a dosage of 100 to 150 mL / mu.

[0017] Optionally, in the application, the medicine is applied once every 6 to 10 days, for a total of 2 to 4 applications.

[0018] The beneficial effects of the present invention are:

[0019] 1. The nano zinc and copper oxychloride used in the present invention are both broad-spectrum fungicides with a wide range of applications, and the compounded composition has a synergistic effect. Nano zinc has a protective and therapeutic effect on plants, can enhance the disease resistance of plants, and improve the tolerance of plants. This feature can further improve the prevention effect, which is significantly higher than the effect of copper oxychloride alone. In addition, it has good compatibility with other chemical pesticides, greatly delays the generation of drug resistance of pathogens, and has high economic and social benefits.

[0020] 2. The principle of the present invention is that the combined bactericide has the function of destroying the protease of pathogens and causing abnormalities in enzymes and other protein mechanisms in bacterial cells, thereby destroying their biofilms and metabolic basis, and ultimately achieving the effect of inhibiting and killing bacteria, with a more efficient bactericidal effect. The present invention expands the bactericidal spectrum of the product, can effectively reduce the dosage and cost of medication, and reduce adverse effects on the environment. DETAILED DESCRIPTION

[0021] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0022] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0023] A combined fungicide comprises: nano zinc and copper oxychloride in a mass ratio of (3-15):(15-35); a pesticide adjuvant and a solvent; the nano zinc is a metal zinc powder with a particle size of less than 100 nm; the copper oxychloride is a powder with a fineness of 2-5 μm.

[0024] Nano zinc is a nano-scale particle of elemental zinc, which is a raw material added to pesticides. The nano-scale zinc oxide it contains has strong bactericidal and antibacterial properties against both Gram-negative bacteria (G-) and Gram-positive bacteria (G+). Gram-negative bacteria are more susceptible to the influence of nano-metal particles due to their relatively thin cell walls, so it has a better antibacterial effect on Gram-negative bacteria. Its antibacterial principle is: by releasing zinc ions and interacting with the negative ions of bacterial cells, it causes abnormal enzymes and other protein mechanisms in bacterial cells, thereby destroying their biofilms and metabolic bases. In addition, zinc ions can affect the DNA replication and transcription of bacteria, hindering their growth and reproduction, and ultimately achieving the effect of inhibiting and killing bacteria. In addition, through the compounding of two broad-spectrum fungicides, nano zinc not only has a bactericidal effect, but also has the effect of improving plant stress resistance, so that a proper reduction in the dosage of copper oxychloride can also achieve a higher prevention and control effect, thereby improving crop quality and yield.

[0025] Optionally, the pesticide adjuvant includes one or more of alkylnaphthalene formaldehyde condensate sulfonate, nonylphenol polyoxyethylene ether (also known as alkylphenol polyoxyethylene ether, TX-10), sodium lignin sulfonate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, sodium dodecyl sulfate, triphenyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether, polyoxyethylene ether sulfate, xanthan gum, methyl ethoxy silicone oil defoamer, magnesium aluminum silicate and urea; including surfactants, defoamers, emulsion stabilizers and other ingredients, which are used to make nano zinc and cuprous oxychloride evenly and stably dispersed in the combined fungicide, and able to form a uniform film on the surface of plant leaves.

[0026] The solvent comprises one or more of water, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, capric amide, methyl oleate and rosin-based vegetable oil; and is used for preparing fungicides of different dosage forms.

[0027] Optionally, the combined fungicide comprises a mass ratio of (2-5): (3-6): (0.1-1):

[0028] (0.1~1) 2-naphthalenesulfonic acid formaldehyde polymer sodium salt, polyoxyethylene ether sulfate, xanthan gum and methyl ethoxy silicone oil defoaming agent, the solvent is water.

[0029] Optionally, the combined fungicide comprises capric amide, nonylphenol polyoxyethylene ether emulsifier (TX-10), fatty alcohol polyoxyethylene ether (AEO-5) and urea in a mass ratio of (10-15): (5-10): (10-15): (3-5), and the solvent is water.

[0030] Optionally, the formulation of the combined fungicide is a common formulation in the pesticide field, including: a soluble agent, an aqueous emulsion, a microemulsion and a suspension.

[0031] Optionally, the mass percentage of the nano zinc and copper oxychloride in the combined fungicide is (3-5) %:

[0032] (30~35)%; the best effect can be achieved within this range.

[0033] An application of the above-mentioned combined fungicide in preventing and controlling rice bacterial leaf streak disease.

[0034] Optionally, the fungicide combination is diluted 500 to 800 times and then sprayed on the rice leaves at a dosage of 100 to 150 mL / mu.

[0035] Optionally, apply the pesticide once every 6 to 10 days, for a total of 2 to 4 applications.

[0036] Example 1

[0037] A combined fungicide comprises the following components in percentage by mass: 3% nano zinc, 30% cuprochlore, 2% 2-naphthalenesulfonic acid formaldehyde polymer sodium salt, 3% polyoxyethylene ether sulfate, 0.3% xanthan gum, 0.3% methylethoxy silicone oil defoamer, and the balance is water.

[0038] Among nano zinc, particles with a diameter between 10-100 nm account for 80.7wt%.

[0039] The preparation method of the combined fungicide of this embodiment includes: mixing all raw materials, stirring them evenly, and then adding them into a sand mill for sand grinding until the material reaches a fineness of 2 to 5 μm. At this time, the fineness of nano zinc remains unchanged while the fineness of copper oxychloride reaches 2 to 5 μm.

[0040] Example 2

[0041] A combined fungicide comprises the following components in percentage by mass: 10% nano zinc, 15% cuprochloric acid, 10% capric amide, 5% nonylphenol polyoxyethylene ether emulsifier (TX-10), 10% fatty alcohol polyoxyethylene ether (AEO-5), 3% urea, and the balance is water.

[0042] The requirements for nano zinc and copper oxychloride are the same as those in Example 1, and the preparation method is the same as that in Example 1.

[0043] Example 3

[0044] A combined fungicide comprises the following components in percentage by mass: 15% nano zinc, 35% cuprochlore, 10% capric amide, 5% nonylphenol polyoxyethylene ether emulsifier (TX-10), 10% fatty alcohol polyoxyethylene ether (AEO-5), 3% urea, and the balance is water.

[0045] The requirements for nano zinc and copper oxychloride are the same as those in Example 1, and the preparation method is the same as that in Example 1.

[0046] Comparative Example 1

[0047] A nano zinc suspension comprises the following components in percentage by mass: 3% nano zinc, 2-naphthalenesulfonic acid formaldehyde polymer sodium salt 2%, 3% polyoxyethylene ether sulfate ester salt, 0.3% xanthan gum, 0.3% methylethoxy silicone oil defoamer, and the balance is water.

[0048] The difference from Example 1 is that copper oxychloride is not added.

[0049] Comparative Example 2

[0050] A cuproximate suspension comprises the following components in percentage by mass: 30% cuproximate, 2-naphthalenesulfonic acid formaldehyde polymer sodium salt 2%, 3% polyoxyethylene ether sulfate ester salt, 0.3% xanthan gum, 0.3% methylethoxy silicone oil defoamer, and the balance is water.

[0051] The difference from Example 1 is that no nano zinc is added.

[0052] Comparative Example 3

[0053] A cuproximate suspension comprises the following components in percentage by mass: 33% cuproximate, 2% 2-naphthalenesulfonic acid formaldehyde polymer sodium salt, 3% polyoxyethylene ether sulfate, 0.3% xanthan gum, 0.3% methylethoxy silicone oil defoamer, and the balance is water.

[0054] The difference from Example 1 is that 3% of the nano zinc is replaced by copper oxychloride.

[0055] Comparative Example 4

[0056] This comparative example uses clean water.

[0057] Example 4

[0058] The efficacy test of the combined fungicide against rice bacterial leaf streak disease was conducted in a test field located in Suqian City, Jiangsu Province, with an area of ​​about 5 hectares. The rice variety was Tsuen-you 532.

[0059] Before applying the pesticide, the disease index was investigated using a five-point investigation method, that is, 5 points were collected in each area, and any 3 points were not concentrated. Ten clusters were investigated at each point, and two leaves on the upper part of the rice plant were investigated in each cluster. A total of 10 leaves were investigated in each area, and the disease level of each leaf was recorded.

[0060] The classification standard refers to "Guidelines for Field Efficacy Tests of Pesticides II" GB / T17980,112-2004:

[0061] Level 0: no lesions on leaves;

[0062] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0063] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0064] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0065] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0066] Level 9: The lesion area accounts for more than 51% of the total leaf area.

[0067] The grading standard is based on the severity of the disease. Each level is expressed in simple numerical values ​​in order of severity, and then calculated using the following formula based on the recorded incidence:

[0068] Disease index = Σ(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9) × 100%.

[0069] The control effect (abbreviated as "control effect") = ((disease index of blank control area - disease index of treated area) / disease index of blank control area) × 100%.

[0070] A randomized block design was carried out in the experimental field, with a plot area of ​​30m 2 , using a backpack sprayer, dilute the set multiples, apply the pesticide twice in the set area and conduct a disease survey 7 days after application. The three disease surveys were conducted on August 1, August 9 and August 17 respectively.

[0071] The control effects are shown in Table 1.

[0072] Table 1

[0073]

[0074]

[0075] It can be seen from the test results in Table 1 that in the first and second groups, the control effect of the nano zinc and copper oxychloride combination on rice bacterial leaf streak disease is both above 75%. The control effect of the first group using Example 1 is 79.86% 7 days after the first application, and the control effect is 87.08% 7 days after the second application; the control effect of the second group using Example 1 is better, that is, within a certain range, the higher the application multiple, the better the control effect, which is significantly higher than comparative examples 1 to 4, especially higher than comparative example 3, indicating that the compound has a synergistic effect compared with the single-dose control agent and the positive control drug, and the combined fungicide can break through the control upper limit of the copper oxychloride single agent with a smaller dose and no adverse reactions such as phytotoxicity occur.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A combined fungicide, characterized in that: include: Nano zinc and copper oxychloride in a mass ratio of (3-15):(15-35), and also including pesticide adjuvant and solvent; The nano zinc is zinc oxide powder with a particle size less than 100 nm; the copper oxychloride is powder with a fineness of 2 to 5 μm.

2. The combined fungicide according to claim 1, characterized in that The pesticide adjuvant includes one or more of alkylnaphthalene formaldehyde condensate sulfonate, nonylphenol polyoxyethylene ether, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether, EO-PO block polyether, sodium dodecyl sulfonate, triphenyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether, polyoxyethylene ether sulfate, xanthan gum, methyl ethoxy silicone oil defoamer, magnesium aluminum silicate and urea, and the solvent includes one or more of water, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, capric amide, methyl oleate and rosin-based vegetable oil.

3. The combined fungicide according to claim 2, characterized in that: The combined bactericide comprises 2-naphthalenesulfonic acid formaldehyde polymer sodium salt, polyoxyethylene ether sulfate, xanthan gum and methylethoxy silicone oil defoamer in a mass ratio of (2-5): (3-6): (0.1-1): (0.1-1), and the solvent is water.

4. The combined fungicide according to claim 2, characterized in that: The combined fungicide comprises capric amide, nonylphenol polyoxyethylene ether emulsifier, fatty alcohol polyoxyethylene ether and urea in a mass ratio of (10-15): (5-10): (10-15): (3-5), and the solvent is water.

5. The combined fungicide according to claim 1, characterized in that: The solvent includes one or more of water, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, capric amide, methyl oleate and rosin-based vegetable oil.

6. The combined fungicide according to claim 1, characterized in that The dosage form of the combined fungicide is one of a solution, an aqueous emulsion, a microemulsion and a suspension.

7. The combined fungicide according to claim 1, characterized in that The mass percentage of the nano zinc and copper oxychloride in the combined fungicide is (3-15)%: (15-35)%.

8. Use of the combined fungicide according to any one of claims 1 to 7 in controlling rice bacterial leaf streak disease.

9. The use according to claim 8, characterized in that After diluting the combined fungicide by 500 to 800 times, the combined fungicide is sprayed on the rice leaves at a dosage of 100 to 150 mL / mu.

10. The use according to claim 8, characterized in that Apply the pesticide once every 6 to 10 days, for a total of 2 to 4 times.

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