Low-endotoxin puffed sterile rat food as well as preparation method and production device thereof

Abstract

The invention belongs to the technical field of puffed food processing, and discloses a low-endotoxin puffed sterile rat food, a preparation method and a production device thereof. The preparation method comprises the following steps: (1) pretreating auxiliary materials to prepare filling slurry; (2) pretreating the main material to prepare puffed main slurry; (3) extruding, puffing and forming to obtain a hollow tubular puffed product; (4) filling slurry is injected, and the filling slurry is gradually injected into the puffed product through an injection mechanism; (5) extruding, cutting and forming: feeding the strip-shaped puffed product into a segmented cutting mechanism, and cutting into puffed particles with equal length; (6) oxidation and detoxification treatment to realize thorough oxidation of endotoxin on the puffed granules; and (7) drying and packaging. According to the preparation method disclosed by the invention, raw material premixing, puffing extrusion molding, continuous grouting and cutting and oxidation detoxification treatment of puffed particles can be sequentially realized, efficient, automatic and continuous production is realized, nutrition balance of the puffed feed is guaranteed, and efficient growth of mice is guaranteed.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and relates to a method for processing puffed food for experimental mice, in particular to a low-endotoxin puffed sterile mouse food, a preparation method and a production device thereof. Background Art

[0002] In recent years, with the rapid development of life sciences, it has become increasingly common to use high-standard animals such as sterile animals, gnotobiotic animals, and SPF animals for scientific experiments. Among them, sterile animals refer to animals that do not have any detectable microorganisms and parasites in their bodies. The breeding of sterile mice mainly adopts the biological purification method, that is, the whole uterus is removed by cesarean section, and the pups are obtained by artificial feeding. One of the keys to successful breeding is whether there is food suitable for the growth and development of sterile mice, including dairy products required for growth and development during lactation and feed required for growth and reproduction. However, there are currently few studies on the nutritional needs of sterile mice, and there are no feed standards for sterile mice during lactation and growth and breeding stages at home and abroad. In addition, the requirements for the breeding and breeding system of sterile mice are extremely strict, and a little mistake will cause the entire sterile system to be wiped out. The highest risk of contamination may be improperly sterilized feed, bedding, and drinking water. Therefore, whether it is the drinking water, bedding, feed or contact items of sterile animals, they must be absolutely sterile. In addition to the living intestinal microbiota, the endotoxins contained in sterile mouse feed can also stimulate the development, expansion and function of the immune system, which may significantly change the experimental results.

[0003] In order to meet the nutritional needs and microbial level requirements of germ-free mice, special feed for sterile mice is generally used. Experimental animal feed can be divided into grain-based compound feed and purified compound feed. The endotoxin contained in grain feed is 100 to 300 times that of purified feed. The ordinary feed (daily diet) fed to sterile mice on a daily basis is specially designed for the metabolic characteristics of sterile mice. The purified feed used for sterile mice is different from the feed fed under general conditions. It requires special extruded feed designed according to the metabolic needs of sterile mice, and the raw materials must be selected with low bacterial load, which makes it easier for sterile mouse feed to be completely sterile.

[0004] In order to meet the needs of sterility, traditional puffed feed for mouse feeding generally adopts steam sterilization, which destroys a large number of nutrients and causes nutritional deficiencies in sterile mice; and because the metabolism of sterile mice changes drastically, the mouse feed used under normal circumstances cannot meet the needs of sterile mice. Moreover, in order to meet the nutritional needs of mouse feeding, the existing puffed feed often needs to add a variety of nutrients, such as protein, fat, carbohydrates, vitamins, minerals, etc. In addition, puffed feed needs to be heated during production and subsequent sterilization and disinfection. Various vitamins, biological enzymes and other nutrient elements added to the raw materials will deteriorate due to the increase in temperature, resulting in reduced enzyme activity and nutrient loss. When designing the formula, the formulator needs to comprehensively consider the stability of the vitamins themselves, the loss during processing and storage, and the additional demand for vitamins by animals under various stress conditions, and reasonably evaluate the amount of vitamins added to the sterile mouse feed to ensure that the sterile mouse feed can meet the nutritional needs of sterile mice after sterilization. Summary of the invention

[0005] The present invention aims to solve the above technical defects of the existing puffed feed for mouse feeding, and provides a low-endotoxin puffed sterile mouse feed, a preparation method and a production device thereof.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] The first aspect of the present invention is to provide a low endotoxin puffed sterile mouse diet, which is prepared by 92-98% of puffed main slurry and 2-8% of filling slurry by mass percentage; wherein the components and dosage ratios of the puffed main slurry and the filling slurry are as follows:

[0008] The puffed main slurry comprises 20-25% casein, 35-40% corn starch, 15-20% wheat starch, 3-8% microcrystalline cellulose, 3-8% sucrose and 2-6% soybean oil, accounting for 20-25% of the total mass of the feed;

[0009] The filling slurry comprises 2-5% of minerals, 0.5-1.5% of vitamins, 0.2-0.4% of methionine, 0.2-0.4% of choline chloride, 0.3-0.5% of lecithin and 0.3-0.8% of oligofructose, accounting for 2-5% of the total mass of the feed.

[0010] Preferably, the expanded main slurry is composed of 93-95% of expanded main slurry and 5-7% of filling slurry by mass percentage, wherein the components and dosage ratios of the expanded main slurry and the filling slurry are as follows:

[0011] The puffed main slurry comprises 22-24% casein, 37-39% corn starch, 17-19% wheat starch, 4-6% microcrystalline cellulose, 4-6% sucrose and 3-5% soybean oil, accounting for 22-24% of the total mass of the feed;

[0012] The filling slurry comprises 3-4% of minerals, 0.8-1.2% of vitamins, 0.25-0.35% of methionine, 0.2-0.3% of choline chloride, 0.35-0.45% of lecithin and 0.4-0.6% of oligofructose, accounting for 3-4% of the total mass of the feed.

[0013] More preferably, the puffed main slurry is prepared by 94.05% of the puffed main slurry and 5.95% of the filling slurry by mass percentage, wherein the components and dosage ratios of the puffed main slurry and the filling slurry are as follows:

[0014] The puffed main slurry comprises 23% casein, 38.85% corn starch, 18.2% wheat starch, 5% microcrystalline cellulose, 5% sucrose and 4% soybean oil, accounting for the total mass of the feed;

[0015] The filling slurry comprises 3.5% of minerals, 1% of vitamins, 0.3% of methionine, 0.25% of choline chloride, 0.4% of lecithin and 0.5% of oligofructose, accounting for the total weight of the feed.

[0016] Preferably, the minerals are composed of ferrous sulfate, potassium iodide and sodium selenite in a mass ratio of 5:2:2.

[0017] Preferably, the vitamins are composed of vitamin C, vitamin E, vitamin B2, vitamin B6, and vitamin D, and the mass ratio thereof is 20:8:5:3:2.

[0018] The second aspect of the present invention is to provide a method for preparing the low-endotoxin expanded sterile mouse feed, comprising the following steps:

[0019] (1) Pretreatment of auxiliary materials: Mix minerals, vitamins, methionine, choline chloride, lecithin and oligofructose to form a nutrient material to prepare a filling slurry for later use;

[0020] (2) Pretreatment of main ingredients: add crushed casein, corn starch, wheat starch, microcrystalline cellulose, sucrose and soybean oil into a mixing mechanism and mix well, add water to perform conditioning at 30-35° C. to obtain a puffed main slurry for later use;

[0021] (3) Extrusion and puffing: Open the solenoid valve at the bottom of the mixing mechanism, add the puffed main slurry after conditioning in step (2) into the puffing machine for extrusion and puffing, and obtain a continuously extruded hollow tubular puffed product;

[0022] (4) injecting filling slurry: feeding the expanded product formed in step (3) into the slurry injection mechanism while it is still hot, and using a plurality of injection mechanisms with annular intervals to inject the filling slurry prepared in step (1) into the expanded product one by one;

[0023] (5) Extrusion and cutting: the strip-shaped expanded product obtained by grouting in step (4) is sent to a segmented cutting mechanism and cut into expanded particles of equal length, and the openings at both ends of the expanded particles are extruded and bonded into a sealed shape, and then cooled to form the expanded particles;

[0024] (6) Oxidation and detoxification treatment: The expanded particles cut and segmented in step (5) are fed into an ultraviolet disinfection device one by one, and the endotoxins on the expanded particles are completely oxidized under the irradiation of a UV-C lamp array;

[0025] (7) Drying and packaging: The expanded particles after oxidation treatment in step (6) are placed in an oven at 45-50°C for drying and packaging.

[0026] Preferably, in step (1), the filling slurry includes 2 to 5 parts of minerals, 0.5 to 1.5 parts of vitamins, 0.2 to 0.5 parts of lecithin, 0.1 to 0.4 parts of choline chloride, 0.3 to 0.8 parts of oligofructose, and 0.1 to 0.3 parts of methionine.

[0027] Preferably, in step (2), the puffed main slurry comprises 15-25 parts of casein, 35-45 parts of corn starch, 5-1 part of wheat starch, 3-8 parts of microcrystalline cellulose, and 5-10 parts of soybean oil.

[0028] Preferably, in step (3), the feeding speed of the front feed port of the extruder is 35-40 Hz, the cutting speed is 20-25 Hz, the screw speed is 120-150 rpm, and the extrusion chamber temperature is 110-115°C.

[0029] The third aspect of the present invention is to provide a low-endotoxin expanded sterile mouse feed production device for the above-mentioned method, comprising a support frame, a mixing mechanism, an expander, a cabinet, a grouting mechanism, a segmented cutting mechanism and an ultraviolet disinfection mechanism, wherein:

[0030] The extruder is horizontally mounted on the support frame, the mixing mechanism is fixedly mounted at the front end of the extruder, and the discharge port at the bottom of the mixing mechanism is directly opposite to the feed port at the front end of the extruder;

[0031] The cabinet is arranged at the downstream position of the support frame, and the grouting mechanism, the segmented cutting mechanism and the ultraviolet disinfection mechanism are installed thereon; and the feeding end of the grouting mechanism is connected to the extruder through a feeding channel, and the discharging end is connected to the ultraviolet disinfection mechanism through a discharging channel via the segmented cutting mechanism.

[0032] Preferably, the mixing mechanism is arranged at the input end of the extruder, a stirring paddle is arranged at the axial position, a discharge port is arranged at the eccentric position of the bottom, and a heating jacket is arranged on the peripheral body, wherein:

[0033] The lower end of the stirring paddle is connected to the stirring motor; the discharge port is a square circular hole, facing the feed port of the extruder; and the upper and lower ends of the heating jacket are respectively provided with a diagonally arranged water inlet and a water outlet.

[0034] Preferably, the puffing machine adopts a twin-screw puffing extruder, which is fixedly mounted on a support frame, and the input end of the extrusion screw inside the extruder is connected to the puffing motor through a coupling.

[0035] Preferably, the cabinet is a rectangular box structure, which is divided into a left box and a right box with an open top by a partition, which are used to install the grouting mechanism and the ultraviolet disinfection mechanism respectively, and support feet are respectively provided at the four corners of the bottom.

[0036] Preferably, the grouting mechanism comprises an annular trough body, an annular guide assembly, a rotating disk, a hollow rotating shaft, a grouting motor and a plurality of grouting needles, wherein:

[0037] The top of the annular trough is open, a supporting column is vertically arranged at the middle axis position, and an arc-shaped grouting channel is arranged at the bottom near the outer periphery, and the front and rear ends of the grouting channel are respectively connected to the feed channel and the discharge channel;

[0038] The annular guide assembly comprises an inner annular guide rail and an outer annular guide rail arranged coaxially, and the inner annular guide rail and the outer annular guide rail are respectively provided with a high-position guide groove and a low-position guide groove of different heights and connected end to end along their circumferential direction;

[0039] The rotating disk is a disc-shaped structure, with a welding hole in the middle and a plurality of needle mounting holes arranged in an annular shape and at equal intervals on the outer periphery, and the welding hole in the middle is fixedly connected to the top end of the hollow rotating shaft;

[0040] The upper and lower ends of the hollow rotating shaft are respectively coaxially rotatably sleeved on the supporting column through bearings, and the bottom end of the hollow rotating shaft is located in the left box body and meshedly connected with the driving gear on the output shaft of the grouting motor;

[0041] A plurality of the grouting needles are limitedly installed in the corresponding needle installation holes, the guide blocks in the middle thereof are movably embedded in the corresponding high-position guide groove or low-position guide groove, and a transversely arranged slurry output pipeline is provided at the top end.

[0042] Preferably, a cylindrical slurry distribution member is fixedly provided at the top of the support column, and a plurality of first distribution holes communicating with the inner cavity of the slurry distribution member are opened at the position of the outer wall of the slurry distribution member corresponding to the low-position guide groove;

[0043] The top of the slurry distribution member is provided with a slurry input pipeline which is sealed and connected to its inner cavity, and a rotatable and sealed slurry distribution ring is coaxially provided on the outer side, and a plurality of second distribution holes connected to the slurry output pipeline are equidistantly provided on the slurry distribution ring body;

[0044] The low-position guide groove is located in the middle position of the grouting channel, and its arc length is at least four lengths of the grouting needle stations. Only the grouting needle located at the position of the low-position guide groove is connected with the inner cavity of the slurry distribution component through the slurry output pipe and the slurry distribution ring to form a grouting passage.

[0045] Preferably, the ring grouting mechanism further comprises a first conveying roller assembly and a second conveying roller assembly respectively arranged at the positions of the feed channel and the discharge channel, wherein:

[0046] The first conveying roller assembly and the second conveying roller assembly are both active conveying rollers driven by a servo motor; and a conveying belt extending to the ultraviolet disinfection mechanism is provided on the discharge channel downstream of the segmented cutting mechanism.

[0047] Preferably, the ultraviolet disinfection mechanism comprises a barrel, a central sleeve, a first UV-C ultraviolet lamp array, a spiral conveying channel and a second UV-C ultraviolet lamp array, wherein:

[0048] The upper end of the barrel body is provided with a feed slideway connected with the discharge channel, the lower end is provided with a discharge slideway, and the top thereof is detachably provided with a barrel cover;

[0049] The central sleeve and the spiral conveying channel are both made of transparent glass, are coaxially arranged at the center of the barrel, and are provided with the first UV-C ultraviolet lamp array;

[0050] The spiral conveying channel is spirally arranged on the inner wall of the barrel body, and its upper and lower ends are respectively connected to the feeding chute and the discharging chute, and a second UV-C ultraviolet lamp array is arranged at its bottom along its length direction.

[0051] The present invention adopts the above technical solution, which has the following technical effects compared with the prior art:

[0052] The method for preparing low-endotoxin puffed sterile mouse feed provided by the present invention ensures balanced nutrition of puffed feed, increases calcium content and ensures efficient growth of mice by adding casein, corn starch, wheat starch, microcrystalline cellulose, sucrose and soybean oil; adopts a grouting mechanism to sequentially fill the extruded puffed feed with a nutrient filling slurry prepared by minerals, vitamins, methionine, choline chloride, lecithin and oligofructose, and adopts a segmented cutting mechanism to cut and press the strip-shaped puffed product to prevent the loss of nutrients; and the puffed particles after cutting and segmenting are sent to an ultraviolet disinfection mechanism one by one, and under the irradiation of a UV-C ultraviolet lamp array, the endotoxin on the puffed particles is completely oxidized to complete the sterilization and disinfection treatment; the production of low-endotoxin puffed sterile mouse feed by adopting the integrated production device can sequentially realize raw material premixing, puffed extrusion molding, continuous grouting, and puffed particle cutting and oxidative detoxification treatment, so as to realize efficient and automatic continuous production. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 This is a schematic diagram of a process for preparing a low-endotoxin expanded sterile mouse feed according to the present invention;

[0054] Figure 2 This is a schematic diagram of the overall structure of a low-endotoxin expanded sterile mouse feed production device of the present invention;

[0055] Figure 3 It is a schematic diagram of the installation structure of a mixing mechanism and an expander in a low-endotoxin expanded sterile mouse feed production device of the present invention;

[0056] Figure 4 It is a schematic diagram of the main structure of a low-endotoxin expanded sterile mouse food production device of the present invention, in which a cabinet, a grouting mechanism, a segmented cutting mechanism and an ultraviolet disinfection mechanism are assembled as one;

[0057] Figure 5 It is a cross-sectional structural schematic diagram of a cabinet, a grouting mechanism, a segmented cutting mechanism and an ultraviolet disinfection mechanism in a low-endotoxin expanded sterile mouse food production device of the present invention, which are assembled as one;

[0058] Figure 6 It is a schematic diagram of a top view of a low-endotoxin expanded sterile mouse food production device of the present invention, in which a cabinet, a grouting mechanism, a segmented cutting mechanism and an ultraviolet disinfection mechanism are assembled as one;

[0059] Figure 7 The three-dimensional structure diagram of the cabinet, grouting mechanism, segmented cutting mechanism and ultraviolet disinfection mechanism in the low-endotoxin expanded sterile mouse food production device of the present invention is integrated into one body. Figure 1 ;

[0060] Figure 8The three-dimensional structure diagram of the cabinet, grouting mechanism, segmented cutting mechanism and ultraviolet disinfection mechanism in the low-endotoxin expanded sterile mouse food production device of the present invention is integrated into one body. Figure 2 ;

[0061] Fig. 9 It is a schematic diagram of a three-dimensional structure in which a cabinet, a grouting mechanism and a segmented cutting mechanism are assembled as one in a low-endotoxin expanded sterile mouse feed production device of the present invention;

[0062] Fig.10 The schematic diagram of the structure of the ultraviolet disinfection mechanism in the low endotoxin expanded sterile mouse food production device of the present invention Figure 1 ;

[0063] Fig.11 The schematic diagram of the structure of the ultraviolet disinfection mechanism in the low endotoxin expanded sterile mouse food production device of the present invention Figure 2 ;

[0064] Fig.12 The schematic diagram of the structure of the ultraviolet disinfection mechanism in the low endotoxin expanded sterile mouse food production device of the present invention Figure 3

[0065] Fig.13 The structure diagram of the annular guide assembly and the rotating disk on the grouting mechanism in the low endotoxin expanded sterile mouse feed production device of the present invention is shown in FIG. Figure 1 ;

[0066] Fig.14 The structure diagram of the annular guide assembly and the rotating disk on the grouting mechanism in the low endotoxin expanded sterile mouse feed production device of the present invention is shown in FIG. Figure 2 ;

[0067] Fig.15 The structure diagram of the annular guide assembly and the rotating disk on the grouting mechanism in the low endotoxin expanded sterile mouse feed production device of the present invention is shown in FIG. Figure 3 ;

[0068] Fig.16 The present invention is a schematic diagram of the structure of a grouting needle on a grouting mechanism in a low-endotoxin expanded sterile mouse feed production device. DETAILED DESCRIPTION

[0069] The present invention is described in detail and specifically by specific examples below to provide a better understanding of the present invention, but the following examples do not limit the scope of the present invention.

[0070] In some embodiments, Figure 1 As shown, a method for preparing a low-endotoxin expanded sterile mouse diet is provided, which specifically comprises the following steps:

[0071] (1) Pretreatment of auxiliary materials: 3.5 parts of minerals, 1 part of vitamins, 0.3 parts of methionine, 0.25 parts of choline chloride, 0.4 parts of lecithin and 0.5 parts of oligofructose are mixed to form a nutrient material to prepare a filling slurry for standby use; the minerals are composed of ferrous sulfate, potassium iodide and sodium selenite, and the mass ratio thereof is 5:2:2; the vitamins are composed of vitamin C, vitamin E, vitamin B2, vitamin B6 and vitamin D, and the mass ratio thereof is 20:8:5:3:2;

[0072] (2) Pretreatment of main ingredients: add 23 parts of crushed casein, 38.85 parts of corn starch, 18.2 parts of wheat starch, 5 parts of microcrystalline cellulose, 5 parts of sucrose and 4 parts of soybean oil to a mixing mechanism 200 and mix well, add water to perform conditioning at 30-35° C. to obtain a puffed main slurry for standby use;

[0073] (3) Extrusion and puffing: Open the solenoid valve at the bottom of the mixing mechanism 200, add the puffed main slurry after conditioning in step (2) into the puffing machine 300 for extrusion and puffing, and obtain a continuously extruded hollow tubular puffed product;

[0074] (4) Injecting filling slurry: The puffed product formed in step (3) is fed into the slurry injection mechanism 500 while hot, and the filling slurry prepared in step (1) is injected into the puffed product one by one using a plurality of injection mechanisms 560 with annular intervals;

[0075] (5) Extrusion and cutting: The strip-shaped puffed product after grouting in step (4) is sent to the segmented cutting mechanism 600 to be cut into puffed particles of equal length, and the openings at both ends of the puffed particles are extruded and bonded into a sealed shape, and then cooled to form the puffed particles;

[0076] (6) Oxidation and detoxification treatment: The expanded particles cut and segmented in step (5) are fed into the ultraviolet disinfection mechanism 700 one by one, and the endotoxins on the expanded particles are completely oxidized under the irradiation of the UV-C ultraviolet lamp array;

[0077] (7) Drying and packaging: The expanded particles after oxidation treatment in step (6) are placed in an oven at 45-50°C for drying and packaging.

[0078] In step (3), the feeding speed of the front feed port of the extruder 300 is 35-40 Hz, the cutting speed is 20-25 Hz, the screw speed is 120-150 rpm, and the extrusion chamber temperature is 110-115°C.

[0079] The preparation method ensures the nutritional balance of the puffed feed, increases the calcium content and ensures the efficient growth of mice by adding casein, corn starch, wheat starch, microcrystalline cellulose, sucrose and soybean oil; a grouting mechanism is used to sequentially fill the extruded puffed feed with a nutrient filling slurry prepared with minerals, vitamins, methionine, choline chloride, lecithin and oligofructose, and a segmented cutting mechanism is used to cut and press the strip-shaped puffed product to prevent the loss of nutrients; and the puffed particles after cutting and segmenting are sent one by one into an ultraviolet disinfection mechanism, and under the irradiation of a UV-C ultraviolet lamp array, the endotoxin on the puffed particles is completely oxidized to complete the sterilization and disinfection treatment.

[0080] In order to realize the continuous and automatic production of the above-mentioned low-endotoxin expanded sterile mouse diet, Figure 2 As shown, the low-endotoxin expanded sterile mouse feed production device used in the preparation method mainly includes a support frame 100, a mixing mechanism 200, an expander 300, a cabinet 400, a grouting mechanism 500, a segmented cutting mechanism 600 and an ultraviolet disinfection mechanism 700.

[0081] like Figure 3 As shown, the extruder 300 is horizontally mounted on the support frame 100, the mixing mechanism 200 is fixedly mounted at the front end of the extruder 300, and the discharge port 203 at the bottom of the mixing mechanism 200 is directly opposite to the feed port at the front end of the extruder 300. After the crushed casein, corn starch, wheat starch, microcrystalline cellulose, sucrose and soybean oil are added to the mixing mechanism 200, the stirring motor 202 is started to stir and mix them thoroughly, and water is added to be tempered at 30-35°C to obtain an expanded main slurry. The obtained expanded main slurry is sent to the extruder 300 below through the discharge port 203 at the bottom of the mixing mechanism 200 for expansion and extrusion.

[0082] like Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 As shown, the cabinet 400 is arranged at the downstream position of the support frame 100. The cabinet 400 and the support frame 100 can be arranged as an integrated structure to form an integrated device as needed. The grouting mechanism 500, the segmented cutting mechanism 600 and the ultraviolet disinfection mechanism 700 are installed at corresponding positions on the cabinet 400 according to the process flow.

[0083] Specifically, the feed end of the grouting mechanism 500 is connected to the extruder 300 through the feed channel 501, and the discharge end is connected to the ultraviolet disinfection mechanism 700 through the discharge channel 503 via the segmented cutting mechanism 600. The extruded product formed by the extruder 300 is sent to the grouting mechanism 500 while hot, and the filling slurry prepared in step 1 is injected into the extruded product one by one by using the injection mechanism 560 with several annular interval steps, and then sent to the segmented cutting mechanism 600 through the discharge channel 503 to be cut into extruded particles of equal length, and the two ends of the extruded particles are opened and squeezed and bonded into a sealed state, and naturally cooled and solidified.

[0084] In some of the embodiments, Figure 2 and Figure 3 As shown, the mixing mechanism 200 is arranged at the input end of the extruder 300, and is used for heating and mixing the main materials. A stirring paddle 201 is arranged at the axis position, and a discharge port 203 is arranged at the eccentric position of the bottom.

[0085] Specifically, the lower end of the stirring paddle 201 is mounted on the barrel body with a bearing seal, and is connected to the stirring motor 202 with a coupling, and the stirring paddle 201 is driven to rotate by the stirring motor 202. In order to improve the feeding efficiency of the extruder 300 and avoid the problem of blockage, the discharge port 203 adopts a square circular hole, which is directly opposite to the feeding port of the extruder 300 and arranged along the length direction of the extruder 300.

[0086] In addition, as needed, a heating jacket 204 is provided on the outer periphery of the mixing mechanism 200, and a temperature probe is provided on the heating jacket 204 for real-time monitoring of the temperature of the heating jacket 204. A diagonally arranged water inlet 205 and a water outlet 206 are provided at the upper and lower ends of the heating jacket 204, respectively, and solenoid valves are provided at the water inlet 205 and the water outlet 206, and circulating water is connected to the outside through hoses respectively.

[0087] In some of the embodiments, Figure 2 and Figure 3 As shown, the puffing machine 300 is a twin-screw puffing extruder, which is fixedly mounted on the support frame 100 , and the input end of the extrusion screw 301 therein is connected to the puffing motor 302 through a coupling 303 .

[0088] In some of the embodiments, Fig. 9As shown, the cabinet 400 is a rectangular box structure, which is welded by steel structure, has a certain supporting strength, and is safe and stable. In order to facilitate the reasonable arrangement of the grouting mechanism 500 and the ultraviolet disinfection mechanism 700, the cabinet 400 is divided into two parts, the left box 401 and the right box 401, which are opened at the top, by a partition, respectively used to install the grouting mechanism 500 and the ultraviolet disinfection mechanism 700, and the four corners of the bottom are respectively provided with support feet 403. The support feet 403 are adjustable support feet, and the horizontality of the cabinet 400 can be adjusted according to the installation ground to ensure smooth installation.

[0089] In some of the embodiments, Figure 5 , Figure 6 , Figure 7 and Figure 8 As shown, the grouting mechanism 500 mainly includes an annular trough body 510, an annular guide assembly 520, a rotating disk 530, a hollow rotating shaft 540, a grouting motor 550 and a plurality of grouting needles 560, and the annular guide assembly 520, the rotating disk 530, the hollow rotating shaft 540 and the plurality of grouting needles 560 are installed in the annular trough body 510.

[0090] Specifically, Fig. 9 As shown, the annular tank body 510 is a circular annular tank body structure with an opening at the top, and a supporting column 511 is vertically arranged at the middle axis position thereof, for installing the rotating disk 530 and the hollow rotating shaft 540. A circular arc-shaped grouting channel 502 is arranged at the bottom of the annular tank body 510 near the outer periphery, and the front and rear ends of the grouting channel 502 are respectively connected to the feed channel 501 and the discharge channel 503. The feed channel 501, the grouting channel 502 and the discharge channel 503 constitute an expanded product conveying passage connecting the extruder 300 and the ultraviolet disinfection mechanism 700 according to the production process.

[0091] like Fig.10 , Fig.11 and Fig.12 As shown, the annular guide assembly 520 includes an inner annular guide rail 522 and an outer annular guide rail 523 arranged coaxially, and a high-position guide groove 524 and a low-position guide groove 524 of different heights and connected end to end are respectively provided on the inner annular guide rail 522 and the outer annular guide rail 523 along their circumference. On the vertical plane, the horizontal height of the high-position guide groove 524 is greater than the horizontal height of the low-position guide groove 524, but on the horizontal plane, the high-position guide groove 524 and the low-position guide groove 524 are connected end to end to form a closed-loop circular guide groove for limiting the installation of the grouting needle 560.

[0092] When in use, the grouting needle 560 circulates along the circular guide groove, and when passing through the high guide groove 524 and the low guide groove 524, the grouting needle 560 rises and falls, and then the needle is lowered or pulled out of the hollow tubular expanded product.

[0093] like Fig.10 , Fig.11 and Fig.12 As shown, the rotating disk 530 is a disc-shaped structure, which is made of aluminum alloy or stainless steel. A welding hole 531 is arranged in the middle, and a plurality of needle mounting holes 532 are arranged in a circular shape and at equal intervals on the outer periphery, which are respectively used for welding and connecting the hollow rotating shaft 540 and installing each grouting needle 560.

[0094] The upper and lower ends of the hollow rotating shaft 540 are respectively coaxially rotatably sleeved on the supporting column 511 through bearings, and the driven gear 541 at the bottom end thereof is located in the left box 401 and meshes with the driving gear 551 on the output shaft of the grouting motor 550. Since the top end of the hollow rotating shaft 540 is fixedly connected to the welding hole 531 in the middle of the rotating disk 530 by welding, when the grouting motor 550 is started, the rotating disk 530 can be synchronously driven to rotate through the hollow rotating shaft 540.

[0095] like Figure 5 , Figure 6 , Figure 7 , Figure 8 and Fig.13 As shown, a plurality of the grouting needles 560 are limitedly installed in the corresponding needle installation holes 532, and the guide blocks 561 in the middle thereof are alternately rotated to the corresponding high guide grooves 524 or low guide grooves 524 when the rotating disk 530 rotates. A limit block 562 is provided at the upper end of each of the grouting needles 560, and there is a certain distance between the limit block 562 and the guide block 561 below, which can meet the lifting space of the grouting needle 560 for up and down movement.

[0096] The top of the grouting needle 560 is provided with a transversely arranged slurry output pipe 563. The inner cavity of the grouting needle 560 is connected with the external filling slurry discharge barrel through the slurry output pipe 563. The filling slurry is injected into the hollow tubular expanded product through a delivery pump to complete the grouting action.

[0097] It is worth noting that Figure 5 and Fig. 9As shown, a cylindrical slurry distributor 512 is welded on the top of the support column 511, and the slurry distributor 512 is a hollow structure. A plurality of first distribution holes connected to the inner cavity are provided at the position of the outer wall of the slurry distributor 512 corresponding to the low-position guide groove 524. A slurry input pipeline 513 connected to the inner cavity is provided at the top of the slurry distributor 512, and a rotatable and sealed slurry distribution ring 514 is coaxially provided on the outside. A plurality of second distribution holes connected to the slurry output pipeline 563 are equidistantly provided on the circumference of the slurry distribution ring 514. The slurry input pipeline 513 is connected to the external filling slurry storage tank through a delivery pump, and the filling slurry storage tank is filled with a nutrient formed by mixing minerals, vitamins, methionine, choline chloride, lecithin and oligofructose.

[0098] The low-position guide groove 524 is located in the middle position of the grouting channel 502, and its arc length is at least four lengths of the grouting needle 560 stations. Only the grouting needle 560 located at the position of the low-position guide groove 524 is connected to the inner cavity of the slurry distribution member 512 through the slurry output pipe 563 and the slurry distribution ring 514 to form a grouting passage.

[0099] During use, when the corresponding grouting needle 560 rotates from the high-position guide groove 524 to the low-position guide groove 524, the needle at the lower end of the grouting needle 560 moves downward to penetrate into the inner cavity of the puffed product, and the second distribution hole at the other end of the grouting needle 560 is connected with the plurality of first distribution holes in sequence, and the slurry is injected into the hollow tubular puffed product through the grouting needle 560; and when the corresponding grouting needle 560 rotates from the high-position guide groove 524 to the low-position guide groove 524, the needle at the lower end of the grouting needle 560 rises and is pulled out from the inner cavity of the puffed product. At this time, the second distribution hole at the other end of the grouting needle 560 rotates to a certain angle and is disconnected from the first distribution hole, and grouting into the inner cavity of the puffed product stops.

[0100] Furthermore, in some of the embodiments, Figure 6 , Figure 7 , Figure 8 and Fig. 9 As shown, the ring grouting mechanism 500 further includes a first conveying roller assembly 504 and a second conveying roller assembly 505 respectively arranged at the positions of the feed channel 501 and the discharge channel 503. The first conveying roller assembly 504 and the second conveying roller assembly 505 both adopt a conventional double-roller conveying structure design, and their specific structure and working principle are known technologies and will not be repeated here.

[0101] As required, the first conveying roller assembly 504 and the second conveying roller assembly 505 are active conveying rollers driven by servo motors and used to pull and convey the strip-shaped puffed product extruded by the puffing machine 300. The strip-shaped puffed product passes between the upper and lower rollers, and the friction between the upper and lower rollers and the strip-shaped puffed product is used to pull and convey the strip-shaped puffed product.

[0102] The segmented cutting mechanism 600 adopts a conventional double-roller cutting device, which also adopts a double-roller conveying structure design. The upper and lower roller surfaces are provided with cutters along their circumferences. The specific structure and working principle are known technologies and will not be described here. The strip-shaped puffed product passes through the upper and lower rollers, and the cutters on the upper and lower rollers are used to cut the strip-shaped puffed product into equal lengths. The cutters and the upper and lower rollers are pressed together to squeeze and bond the two ends of the puffed particles into a sealed shape, and solidify and form after cooling.

[0103] A conveyor belt extending to the ultraviolet disinfection mechanism 700 is provided on the discharge channel 503 downstream of the segmented cutting mechanism 600. The conveyor belt is a transmission belt for conveying the expanded particles cut by the segmented cutting mechanism 600 into the ultraviolet disinfection mechanism 700 for oxidation and detoxification.

[0104] In some of the embodiments, Figure 5 , Fig.14 , Fig.15 and Fig.16 As shown, the ultraviolet disinfection mechanism 700 is used as an oxidative detoxification device for expanded particles, which mainly includes a barrel 701, a central sleeve 702, a first UV-C ultraviolet lamp array 703, a spiral conveying channel 704 and a second UV-C ultraviolet lamp array 705.

[0105] The upper end of the barrel body 701 is provided with a feed slide 706 connected with the discharge channel 503, the lower end is provided with a discharge slide 707, and the top thereof is detachably provided with a barrel cover 708. The barrel cover 708 is provided with a transparent observation window 709 and a handle 710.

[0106] In order to improve the ultraviolet disinfection effect of the expanded particles, the central sleeve 702 and the spiral conveying channel 704 are both made of transparent glass, which are coaxially arranged at the center of the barrel 701, and the first UV-C ultraviolet lamp array 703 is arranged therein. The ultraviolet light of the first UV-C ultraviolet lamp array 703 passes through the central sleeve 702 and irradiates the expanded particles from the side.

[0107] The spiral conveying channel 704 is spirally arranged on the inner wall of the barrel 701, and its upper and lower ends are respectively connected to the feeding chute 706 and the discharging chute 707, and the bottom thereof is provided with a second UV-C ultraviolet lamp array 705 along its length direction. By laying the second UV-C ultraviolet lamp array 705 on the bottom of the spiral conveying channel 704, it is convenient for the ultraviolet light of the second UV-C ultraviolet lamp array 705 to irradiate the expanded particles from above, thereby achieving sufficient oxidation sterilization and disinfection of the expanded particles.

[0108] The spiral conveying channel 704 in the ultraviolet disinfection mechanism 700 adopts a spiral structure design, and a first UV-C ultraviolet lamp array 703 is arranged at the inner center position, and a second UV-C ultraviolet lamp array 705 is laid at the bottom of the spiral conveying channel 704, so as to realize ultraviolet irradiation of the expanded feed in different directions and ensure that there is no dead angle for irradiation. At the same time, the oxidation disinfection time of the expanded particles in the ultraviolet disinfection mechanism 700 is prolonged to a certain extent, and the irradiation dose of the first UV-C ultraviolet lamp array 703 and the spiral conveying channel 704 can be appropriately reduced as needed to avoid damage to other nutrients.

[0109] The low-endotoxin puffed sterile mouse food production device is mainly composed of a support frame 100, a mixing mechanism 200, an extruder 300, a cabinet 400, a grouting mechanism 500, a segmented cutting mechanism 600 and an ultraviolet disinfection mechanism 700, forming an integrated production equipment. The low-endotoxin puffed sterile mouse food production device can sequentially realize raw material premixing, puffing extrusion molding, continuous grouting, and cutting and oxidative detoxification treatment of puffed particles, thereby realizing efficient, automatic and continuous production.

[0110] The specific embodiments of the present invention are described in detail above, but they are only examples, and the present invention is not limited to the specific embodiments described above. For those skilled in the art, any equivalent modifications and substitutions made to the present invention are also within the scope of the present invention. Therefore, the equalization changes and modifications made without departing from the spirit and scope of the present invention should be included in the scope of the present invention.

Claims

1. A low-endotoxin expanded sterile mouse diet, characterized in that: The expanded main slurry is prepared by 92-98% of the expanded main slurry and 2-8% of the filling slurry by mass percentage; wherein the components and dosage ratios of the expanded main slurry and the filling slurry are as follows: The puffed main slurry comprises 20-25% casein, 35-40% corn starch, 15-20% wheat starch, 3-8% microcrystalline cellulose, 3-8% sucrose and 2-6% soybean oil, accounting for 20-25% of the total mass of the feed; The filling slurry comprises 2-5% of minerals, 0.5-1.5% of vitamins, 0.2-0.4% of methionine, 0.2-0.4% of choline chloride, 0.3-0.5% of lecithin and 0.3-0.8% of oligofructose, accounting for 2-5% of the total mass of the feed.

2. The method for preparing the low-endotoxin expanded sterile mouse diet according to claim 1, characterized in that: In step (1), the expanded main slurry is composed of 94.05% of the expanded main slurry and 5.95% of the filling slurry by mass percentage, wherein the components and dosage ratios of the expanded main slurry and the filling slurry are as follows: The puffed main slurry comprises 23% casein, 38.85% corn starch, 18.2% wheat starch, 5% microcrystalline cellulose, 5% sucrose and 4% soybean oil, accounting for the total mass of the feed; The filling slurry comprises 3.5% of minerals, 1% of vitamins, 0.3% of methionine, 0.25% of choline chloride, 0.4% of lecithin and 0.5% of oligofructose, accounting for the total weight of the feed.

3. A method for preparing the low-endotoxin expanded sterile mouse feed as claimed in claim 1 or 2, characterized in that: The steps include: (1) Pretreatment of auxiliary materials: Mix minerals, vitamins, methionine, choline chloride, lecithin and oligofructose to form a nutrient material to prepare a filling slurry for later use; (2) Pretreatment of the main ingredients: adding the crushed casein, corn starch, wheat starch, microcrystalline cellulose, sucrose and soybean oil to the mixing mechanism (200) and mixing them evenly, adding water to perform conditioning at 30-35° C. to obtain a puffed main slurry for later use; (3) Extrusion and puffing: opening the electromagnetic valve at the bottom of the mixing mechanism (200), adding the puffed main slurry after conditioning in step (2) into the puffing machine (300), extruding and puffing, and obtaining a continuously extruded hollow tubular puffed product; (4) injecting filling slurry: feeding the puffed product formed in step (3) into the slurry injection mechanism (500) while hot, and using a plurality of injection mechanisms (560) with annular intervals to inject the filling slurry prepared in step (1) into the puffed product one by one; (5) Extrusion and cutting: the strip-shaped puffed product after grouting in step (4) is sent to the segmented cutting mechanism (600) to be cut into puffed particles of equal length, and the openings at both ends of the puffed particles are extruded and bonded into a sealed shape, and then cooled to form the puffed particles; (6) Oxidation and detoxification treatment: the expanded particles cut and segmented in step (5) are fed one by one into the ultraviolet disinfection mechanism (700) to achieve complete oxidation of the endotoxins on the expanded particles under the irradiation of the UV-C ultraviolet lamp array; (7) Drying and packaging: The expanded particles after oxidation treatment in step (6) are placed in an oven at 45-50°C for drying and packaging.

4. The method for preparing the low-endotoxin expanded sterile mouse diet according to claim 3, characterized in that: In step (3), the feeding speed of the front feed port of the extruder (300) is 35-40 Hz, the cutting speed is 20-25 Hz, the screw speed is 120-150 rpm, and the extrusion chamber temperature is 110-115°C.

5. A low-endotoxin expanded sterile mouse feed production device for the preparation method according to claim 3 or 4, characterized in that: The invention comprises a support frame (100), a mixing mechanism (200), an expander (300), a cabinet (400), a grouting mechanism (500), a segmented cutting mechanism (600) and an ultraviolet disinfection mechanism (700), wherein: The expansion machine (300) is transversely mounted on the support frame (100), the mixing mechanism (200) is fixedly mounted at the front end of the expansion machine (300), and the discharge port (203) at the bottom of the mixing mechanism (200) is directly opposite to the feed port at the front end of the expansion machine (300); The cabinet (400) is arranged at a downstream position of the support frame (100), and is provided with the grouting mechanism (500), the segmented cutting mechanism (600) and the ultraviolet disinfection mechanism (700); and the feed end of the grouting mechanism (500) is connected to the extruder (300) through a feed channel (501), and the discharge end is connected to the ultraviolet disinfection mechanism (700) through a discharge channel (503) via the segmented cutting mechanism (600).

6. The low-endotoxin expanded sterile mouse feed production device according to claim 5, characterized in that: The mixing mechanism (200) is arranged at the input end of the extruder (300), a stirring paddle (201) is arranged at the axis position, a discharge port (203) is arranged at the eccentric position of the bottom, and a heating jacket (204) is arranged on the outer periphery, wherein: The lower end of the stirring paddle (201) is connected to the stirring motor (202); the discharge port (203) is a square circular hole, facing the feed port of the expander (300); and the upper and lower ends of the heating jacket (204) are respectively provided with a diagonally arranged water inlet (205) and a water outlet (206).

7. The low-endotoxin expanded sterile mouse feed production device according to claim 5, characterized in that: The puffing machine (300) is a twin-screw puffing extruder, which is fixedly mounted on a support frame (100), and the input end of the extrusion screw (301) therein is connected to the puffing motor (302) via a coupling (303).

8. The low-endotoxin expanded sterile mouse feed production device according to claim 5, characterized in that: The cabinet (400) is a rectangular box structure, which is divided into a left box (401) and a right box (401) with an opening at the top by a partition, and is used to install the grouting mechanism (500) and the ultraviolet disinfection mechanism (700), respectively, and support feet (403) are respectively provided at the four corners of the bottom.

9. The low-endotoxin expanded sterile mouse feed production device according to claim 5, characterized in that: The grouting mechanism (500) comprises an annular groove body (510), an annular guide assembly (520), a rotating disk (530), a hollow rotating shaft (540), a grouting motor (550) and a plurality of grouting needles (560), wherein: The annular groove body (510) is open at the top, a supporting column (511) is vertically arranged at the middle axis position, and an arc-shaped grouting channel (502) is arranged at the bottom near the outer periphery, and the front and rear ends of the grouting channel (502) are respectively connected to the feed channel (501) and the discharge channel (503); The annular guide assembly (520) comprises an inner annular guide rail (522) and an outer annular guide rail (523) arranged coaxially, and the inner annular guide rail (522) and the outer annular guide rail (523) are respectively provided with a high-position guide groove (524) and a low-position guide groove (524) of different heights and connected end to end along their circumferential direction; The rotating disk (530) is a disc-shaped structure, with a welding hole (531) provided in the middle thereof, and a plurality of needle mounting holes (532) arranged in an annular shape and at equal intervals are provided on the outer periphery, wherein the welding hole (531) in the middle thereof is fixedly connected to the top end of the hollow rotating shaft (540); The upper and lower ends of the hollow rotating shaft (540) are respectively coaxially rotatably sleeved on the supporting column (511) through bearings, and the driven gear (541) at the bottom end thereof is located in the left box (401) and meshes with the driving gear (551) on the output shaft of the grouting motor (550); A plurality of the grouting needles (560) are limitedly installed in the corresponding needle installation holes (532), wherein the guide block (561) in the middle is movably embedded in the corresponding high-position guide groove (524) or low-position guide groove (524), and a slurry output pipe (563) arranged transversely is provided at the top.

10. The low-endotoxin expanded sterile mouse feed production device according to claim 9, characterized in that: A cylindrical slurry distribution piece (512) is fixedly provided at the top of the support column (511), and a plurality of first distribution holes communicating with the inner cavity of the slurry distribution piece (512) are provided on the outer wall of the slurry distribution piece (512) at positions corresponding to the low-position guide groove (524); The top of the slurry distribution member (512) is provided with a slurry input pipe (513) which is sealed and connected to its inner cavity, and a rotatable and sealed slurry distribution ring (514) is coaxially provided on the outside, and a plurality of second distribution holes connected to the slurry output pipe (563) are equidistantly provided on the circumference of the slurry distribution ring (514); The low-position guide groove (524) is located in the middle of the grouting channel (502), and its arc length is at least the length of four grouting needle heads (560) working stations. Only the grouting needle heads (560) located at the low-position guide groove (524) are connected to the inner cavity of the slurry distribution member (512) through the slurry output pipe (563) and the slurry distribution ring (514), thereby forming a grouting passage.

11. The low-endotoxin expanded sterile mouse feed production device according to claim 9, characterized in that: The grouting mechanism (500) further comprises a first conveying roller assembly (504) and a second conveying roller assembly (505) respectively arranged at the positions of the feeding channel (501) and the discharging channel (503), wherein: The first conveying roller assembly (504) and the second conveying roller assembly (505) are both active conveying rollers driven by a servo motor; and a conveying belt extending to the ultraviolet disinfection mechanism (700) is provided on the discharge channel (503) downstream of the segmented cutting mechanism (600).

12. The low-endotoxin expanded sterile mouse feed production device according to claim 5, characterized in that: The ultraviolet disinfection mechanism (700) comprises a barrel (701), a central sleeve (702), a first UV-C ultraviolet lamp array (703), a spiral conveying channel (704) and a second UV-C ultraviolet lamp array (705), wherein: The upper end of the barrel body (701) is provided with a feed slideway (706) connected to the discharge channel (503), the lower end is provided with a discharge slideway (707), and the top thereof is detachably provided with a barrel cover (708); The central sleeve (702) and the spiral conveying channel (704) are both made of transparent glass, are coaxially arranged at the center of the barrel (701), and the first UV-C ultraviolet lamp array (703) is arranged therein; The spiral conveying channel (704) is spirally arranged on the inner wall of the barrel (701), and its upper and lower ends are respectively connected to the feeding chute (706) and the discharging chute (707), and a second UV-C ultraviolet lamp array (705) is arranged at its bottom along its length direction.

Images