Lutein ester oil suspension, its preparation method and application

Abstract

The present application relates to the technical field of food additive, in particular to a lutein ester oil suspension, a preparation method and application thereof. The lutein ester oil suspension prepared by the preparation method has good stability and coloring effect, the product is colored and colored uniformly and stably, and the color and luster of products such as batter can be significantly improved. The method does not use organic solvents, is green and environmentally friendly, has the characteristics of simple process, convenient use, strong applicability, and is easy to mass industrial production and application.

Description

Technical Field

[0001] The present invention relates to the technical field of food additives, and in particular to a lutein ester oil suspension and a preparation method and application thereof. Background Art

[0002] Lutein is a natural carotenoid found in a wide variety of plants, particularly in dark-colored vegetables and fruits. Lutein possesses strong antioxidant properties, protecting the eyes from blue light damage and reducing the risk of cataracts and macular degeneration. Lutein also exhibits numerous biological activities, including anti-cancer, anti-inflammatory, and immunomodulatory activities. Therefore, lutein holds great promise for application in food, pharmaceuticals, and cosmetics. However, lutein's poor stability and susceptibility to oxidative degradation have limited its application. To address this issue, researchers have developed lutein derivatives—lutein esters—combined with one or two fatty acid molecules, which exhibit improved stability. Existing lutein esters are primarily extracted from plants and typically formulated into oils or aqueous suspensions by adding appropriate solvents and emulsifiers for ease of use. These lutein ester products are commonly used as food additives to improve product color and nutritional quality. However, the preparation of existing lutein ester oil suspensions is complex and requires the use of large amounts of organic solvents, which is both costly and environmentally harmful. In addition, lutein ester oil suspensions are prone to physical stratification, color change, and loss of active ingredients during storage and use, and their stability needs to be improved. These problems limit their widespread application.

[0003] CN201010602338.6 discloses a carotenoid oil suspension with high bioavailability and a preparation method thereof. The method comprises dissolving carotenoids in a mixed organic solvent and atomizing and concentrating the mixture to obtain the carotenoid oil suspension. The preparation process requires the use of a large amount of organic solvent, which is not only costly but also environmentally polluting.

[0004] CN202211328321.5 discloses a beta-carotene oil suspension and its preparation method and application, which prepares a beta-carotene oil suspension with a content of more than 30% by a grinding and pulverization method, with good fluidity, simple preparation process, and convenient large-scale production. However, unlike beta-carotene, lutein ester is unstable when heated after mixing with oils and fats due to its fatty acid structure, and is easily dissolved and separated, and the mechanical force impact force is large during grinding, and lutein ester crystals are also easily precipitated from the system. Therefore, the above method is more difficult to be applied to the preparation of lutein ester oil suspension.

[0005] In addition to the need to ensure product stability, lutein ester is used in the field of food and the like, color tone stability and coloring effect also need to be concerned. Although the prior art prepares lutein ester into a certain form of preparation to improve its oil solubility, it pays little attention to the stability and coloring effect of lutein ester preparation. Therefore, it is urgent to develop a lutein ester oil suspension which is stable, non-laminated and has stable and uniform coloring. SUMMARY

[0006] In order to overcome the problems of lutein ester oil suspension in the prior art, the present application provides a lutein ester oil suspension, a preparation method and application thereof.

[0007] In the research process, it is found that the product prepared by simply mixing and homogenizing lutein ester crystals and oil is unstable in color, and the color gradually changes from red to orange of different degrees during storage, affecting the coloring effect. In addition, the crystals precipitate and sink, and the oil floats up, which is easy to be laminated. After continuous attempts, it is found that by controlling the lutein ester content of lutein ester crystals and adding oil-soluble emulsifiers compounded by medium-chain triglycerides and glycerol fatty acid esters in the system, the stability of lutein ester crystals in the system can be significantly improved, and the possibility of precipitation and stratification under normal temperature conditions can be reduced. At the same time, it is also found that by using programmed cooling control and emulsification homogenization technology, the particle size and its distribution of lutein ester crystals in the system can be effectively controlled. Combined with the above-mentioned compounded emulsifiers, the color of the product can be controlled, and the stability of the product can be ensured, solving the problems of storage stratification and color change. In summary, the lutein ester oil suspension prepared by the preparation method provided by the present application has both stability and good coloring effect.

[0008] Specifically, the present application provides the technical solutions described below.

[0009] The present application provides a preparation method of lutein ester oil suspension, which comprises:

[0010] The lutein ester crystals are mixed with a first composition, and then heated to dissolve the crystals to obtain a second composition, wherein the first composition comprises oil, a composite emulsifier and a compounded antioxidant;

[0011] The second composition is subjected to programmed cooling in two stages to obtain a third composition;

[0012] The third composition is subjected to emulsification and homogenization;

[0013] The lutein ester content of the lutein ester crystals is 65%-80%;

[0014] The composite emulsifier comprises medium-chain triglycerides and glycerol fatty acid esters in a mass ratio of (60-74):(2-4).

[0015] The present application controls the lutein ester content of lutein ester crystals between 65%-80% through verification experiments, and the stability, particle size and distribution, and coloring effect of lutein ester oil suspension can be significantly improved by adding the above-mentioned medium-chain triglycerides and glycerol fatty acid esters as emulsifiers in the above-mentioned mass ratio. The above-mentioned raw material improvement, heating and dissolving the crystals, and the subsequent programmed cooling can further improve the stability, particle size and distribution, and coloring effect of the lutein ester oil suspension.

[0016] In the above-mentioned method, the lutein ester crystals, oil, composite emulsifier, and composite antioxidant are first dissolved and dispersed under certain temperature conditions to obtain an oil solution of lutein ester, and then the solution is cooled step by step according to a specific cooling program to obtain a suspension, and finally the suspension is emulsified and homogenized to obtain a uniform and stable lutein ester oil suspension. The purpose of the two-stage programmed cooling after heating and dissolving the lutein ester crystals is to induce low-temperature phase transition. The present application finds that the color brightness of the prepared oil suspension is reduced, the particle size is larger and the distribution is uneven, and the product stability is reduced if the crystals are not dissolved at high temperature or the two-step programmed cooling is adjusted to one-step cooling.

[0017] The lutein ester content of the lutein ester crystals used in the present application is preferably between 65%-75%. The lutein ester crystals with the above-mentioned content, when mixed with oil, emulsifiers, etc. in a suitable ratio, can help to form a more stable oil suspension.

[0018] Medium-chain triglycerides (MCT) are medium-chain fatty acid triglycerides, and the main components are saturated caprylic acid triglyceride, saturated capric acid triglyceride, and saturated caprylic acid-capric acid mixed triglyceride.

[0019] The HLB value of the glycerol fatty acid ester used in the present application is preferably 3.0-3.5. A low HLB value and a suitable addition ratio of glycerol fatty acid ester can better ensure that the hydrophilic groups in the emulsifier do not affect the solubility of lutein ester in oil, and can effectively disperse oil droplets, better ensuring that the product does not separate during storage.

[0020] Preferably, the glycerol fatty acid ester is at least one selected from glycerol stearate, glycerol distearate, glycerol oleate, and glycerol dioleate.

[0021] In some embodiments of the present application, the glycerol fatty acid ester is glycerol dioleate.

[0022] Preferably, the mass ratio of medium-chain triglycerides to glycerol fatty acid ester is (66-74):(3-4).

[0023] In the above-mentioned method, the two-stage programmed cooling includes:

[0024] The first-stage programmed cooling is preferably at a rate of 15-20°C / h to a temperature of 30-40°C.

[0025] The second-stage programmed cooling is preferably at a rate of 15-25°C / h to a temperature of 5-15°C.

[0026] The first-stage programmed cooling is preferably at a rate of 15-20°C / h to a temperature of 30-40°C, and the second-stage programmed cooling is preferably at a rate of 15-25°C / h to a temperature of 10-15°C.

[0027] The stirring speed in the first-stage programmed cooling is preferably 30-50 rpm, and the stirring speed in the second-stage programmed cooling is preferably 80-120 rpm.

[0028] The stirring time in the first-stage programmed cooling is preferably 1-2 h, and the stirring time in the second-stage programmed cooling is preferably 1.5-2 h. If the time for cooling to the final temperature is less than the stirring time, the stirring is continued at the final temperature until the stirring time.

[0029] The starting temperature of the first-stage programmed cooling is preferably 50-70°C (more preferably 60-70°C). The starting temperature of the second-stage programmed cooling is the final temperature of the first-stage programmed cooling.

[0030] In the above method, the conditions for heating and dissolving the crystals are a temperature of 50-70°C (more preferably 60-70°C) and a stirring speed of 200-300 rpm.

[0031] The time for heating and dissolving is preferably 1-4 h.

[0032] The oil used in the present application is preferably a vegetable oil. Further preferably, the oil is at least one selected from the group consisting of soybean oil, sunflower oil, corn oil and peanut oil.

[0033] In some embodiments of the present application, the oil is soybean oil.

[0034] The complex antioxidant used in the present application is preferably vitamin E and rosemary extract.

[0035] More preferably, the mass ratio of vitamin E to rosemary extract is 2: (0.5-1).

[0036] More preferably, the vitamin E is natural vitamin E.

[0037] In the above method, the mixing ratio of the first composition to the lutein ester crystals is 12.5-23 parts of lutein ester crystals, 5-10 parts of oil, 62-78 parts of complex emulsifier and 2-4 parts of complex antioxidant.

[0038] Preferably, the mixing ratio of the first composition and the lutein ester crystals is: lutein ester crystals 13.5-20 parts, oil and fat 6-8 parts, complex emulsifier 69-78 parts, and compound antioxidant 2-4 parts.

[0039] In the above method, the emulsification conditions are preferably: an emulsification rate of 3000-4000 rpm and an emulsification time of 30-40 min.

[0040] In the above method, the homogenization pressure is preferably 320-380 bar.

[0041] The preparation system of the lutein ester oil suspension provided by the present application introduces a specific complex emulsifier, which can effectively improve the solubility and stability of lutein ester in oil and fat. By performing low-temperature induction to induce phase transition of the system, the lutein ester and other components in the system form a suspension, and then emulsification and homogenization are performed to further improve the stability and uniformity of the oil suspension. The lutein ester oil suspension product prepared by the method has good stability and color tone, and has the characteristics of golden color when applied in food such as breading.

[0042] The present application also provides a lutein ester oil suspension prepared by the above-mentioned method for preparing a lutein ester oil suspension.

[0043] Preferably, the lutein ester content of the lutein ester oil suspension is 10%-15%. The particle size D50 of the lutein ester oil suspension is between 8-10 μm, and the span (PDI) is less than 0.5.

[0044] The preparation method of the present application can control the D50 particle size of lutein ester in the system to be between 8-10 μm, and the span is less than 0.5, so that the product presents a uniform and stable orange yellow oil suspension state. The appearance color tone value of the prepared lutein ester oil suspension is L*=45-55, a*=30-40, and b*=30-50 (wherein, L* represents brightness, 0 is black, 100 is white, and the larger the L* value, the higher the brightness; a* represents red and green, positive value is red, and negative value is green; b* represents yellow and blue, positive value is yellow, and negative value is blue). In addition, the lutein ester oil suspension prepared by the method of the present application has good stability, and the shelf life (6 months) stability is greater than 95% (stability=upper oil layer height / total height*100%).

[0045] The present application provides the use of the above-mentioned lutein ester oil suspension in the preparation of food, medicine, cosmetics or feed.

[0046] Illustratively, the food is breading.

[0047] The lutein ester oil suspension provided by the application is applied to breading powder, when the adding amount is 2.5‰ (the adding amount is based on the content of 10% of the lutein ester oil suspension), the color tone value of the breading powder is L*=90-94, a*=0-3, b*=30-35, and the breading powder is golden yellow.

[0048] The lutein ester oil suspension prepared by the preparation method provided by the application has good stability and coloring effect, the product is colored and colored uniformly and stably, and the color of the breading powder and other products can be significantly increased. DETAILED DESCRIPTION

[0049] To make the purpose, technical scheme and advantages of the application clearer, the technical scheme in the application will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the application, rather than all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application.

[0050] The raw materials such as medium-chain triglyceride used in the following embodiments can be purchased through the market.

[0051] Embodiment 1

[0052] The lutein ester oil suspension provided by the embodiment comprises the following raw materials: lutein ester crystal (total lutein ester content 75%) 13.5 kg, soybean oil 6 kg, medium-chain triglyceride 73.5 kg, glycerol dioleate 4 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0053] The embodiment further provides a preparation method of the lutein ester oil suspension, and the specific steps are as follows:

[0054] 1) Dissolve the soybean oil, medium-chain triglyceride, glycerol dioleate, vitamin E and rosemary extract uniformly at 60℃ to obtain composition 1;

[0055] 2) Add the lutein ester crystal into composition 1, and stir and dissolve at 60℃ and 300 rpm for 2.5 h to make the lutein ester uniformly dispersed in the system to obtain composition 2;

[0056] 3) cooling the composition 2 by two-stage program to obtain composition 3; wherein, the first stage program cooling rate is 15℃ / h, cooling to 30℃, stirring speed is 50rpm, stirring time is 2h; the second stage program cooling rate is 20℃ / h, cooling to 10℃, stirring speed is 100rpm, stirring time is 1.5h;

[0057] 4) shearing emulsification of the composition 3, emulsification speed is 4000rpm, emulsification time is 30min, and finally homogenized under 350bar to obtain the lutein ester oil suspension.

[0058] Example 2

[0059] The embodiment provides a lutein ester oil suspension, and raw material components of the lutein ester oil suspension are as follows: lutein ester crystal (total lutein ester content is 75%) 20kg, soybean oil 8kg, medium-chain triglyceride 66kg, glycerol dioleate 3kg, vitamin E 2kg, rosemary extract 1kg.

[0060] The embodiment further provides a preparation method of the lutein ester oil suspension, and the preparation method is the same as the preparation method in the example 1.

[0061] Example 3

[0062] The embodiment provides a lutein ester oil suspension, and raw material components of the lutein ester oil suspension are as follows: lutein ester crystal (total lutein ester content is 65%) 15.4kg, soybean oil 6kg, medium-chain triglyceride 71.6kg, glycerol dioleate 4kg, vitamin E 2kg, rosemary extract 1kg.

[0063] The embodiment further provides a preparation method of the lutein ester oil suspension, and the preparation method is the same as the preparation method in the example 1.

[0064] Example 4

[0065] The embodiment provides a lutein ester oil suspension, and raw material components of the lutein ester oil suspension are as follows: lutein ester crystal (total lutein ester content is 75%) 13.5kg, soybean oil 6kg, medium-chain triglyceride 73.5g, glycerol dioleate 4g, vitamin E 2g, rosemary extract 1g.

[0066] The embodiment further provides a preparation method of the lutein ester oil suspension, and specific steps are as follows:

[0067] 1) dissolving and uniformly mixing soybean oil, medium-chain triglyceride, glycerol dioleate, vitamin E and rosemary extract under the condition of 60℃ to obtain composition 1;

[0068] 2) Add lutein ester crystals into composition 1, stir and dissolve at 70℃, 300rpm for 2h to make lutein ester evenly dispersed in the system, to obtain composition 2;

[0069] 3) Composition 2 is subjected to two-stage programmed cooling to obtain composition 3; the first-stage programmed cooling has a cooling rate of 20℃ / h, and is cooled to 30℃, with a stirring speed of 50rpm and a stirring time of 2h; the second-stage programmed cooling has a cooling rate of 15℃ / h, and is cooled to 10℃, with a stirring speed of 100rpm and a stirring time of 1.5h;

[0070] 4) Composition 3 is subjected to shear emulsification, with an emulsification speed of 4000rpm and an emulsification time of 30min, and finally homogenized at a pressure of 350bar to obtain a lutein ester oil suspension.

[0071] Example 5

[0072] This example provides a lutein ester oil suspension, which is composed of lutein ester crystals (total lutein ester content 75%) 13.5kg, soybean oil 6kg, medium-chain triglyceride 74kg, glycerol dioleate 4kg, vitamin E 2kg, rosemary extract 0.5kg.

[0073] This example further provides a preparation method of the lutein ester oil suspension, and the specific steps are as follows:

[0074] 1) Dissolve and homogenize soybean oil, medium-chain triglyceride, glycerol dioleate, vitamin E and rosemary extract at 60℃ to obtain composition 1;

[0075] 2) Add lutein ester crystals into composition 1, stir and dissolve at 60℃, 300rpm for 1h to make lutein ester evenly dispersed in the system, to obtain composition 2;

[0076] 3) Composition 2 is subjected to two-stage programmed cooling to obtain composition 3; the first-stage programmed cooling has a cooling rate of 20℃ / h, and is cooled to 40℃, with a stirring speed of 40rpm and a stirring time of 1.5h; the second-stage programmed cooling has a cooling rate of 25℃ / h, and is cooled to 15℃, with a stirring speed of 120rpm and a stirring time of 2h;

[0077] 4) Composition 3 is subjected to shear emulsification, with an emulsification speed of 3000rpm and an emulsification time of 40min, and finally homogenized at a pressure of 380bar to obtain a lutein ester oil suspension.

[0078] Comparative Example 1

[0079] The comparative example 1 provides a lutein ester oil suspension, which is different from the raw material composition of the lutein ester oil suspension of example 1 in that: lutein ester crystal (total lutein ester content 85%) 11.9 kg, soybean oil 7.6 kg, medium-chain triglyceride 73.5 kg, glycerol dioleate 4 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0080] The preparation method of the above lutein ester oil suspension is the same as that of example 1.

[0081] Comparative example 2

[0082] The comparative example 1 provides a lutein ester oil suspension, which is different from the raw material composition of the lutein ester oil suspension of example 1 in that: lutein ester crystal (total lutein ester content 62%) 16.3 kg, soybean oil 3.2 kg, medium-chain triglyceride 73.5 kg, glycerol dioleate 4 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0083] The preparation method of the above lutein ester oil suspension is the same as that of example 1.

[0084] Comparative example 3

[0085] The comparative example 1 provides a lutein ester oil suspension, which is different from the raw material composition of the lutein ester oil suspension of example 1 in that: lutein ester crystal (total lutein ester content 75%) 13.5 kg, soybean oil 6 kg, medium-chain triglyceride 69.5 kg, glycerol dioleate 8 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0086] The preparation method of the above lutein ester oil suspension is the same as that of example 1.

[0087] Comparative example 4

[0088] The comparative example 1 provides a lutein ester oil suspension, which is different from the raw material composition of the lutein ester oil suspension of example 1 in that: lutein ester crystal (total lutein ester content 75%) 13.5 kg, soybean oil 6 kg, medium-chain triglyceride 73.5 kg, Span 80 4 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0089] The preparation method of the above lutein ester oil suspension is the same as that of example 1.

[0090] Comparative example 5

[0091] This comparative example provides a lutein ester oil suspension, which is different from the raw material composition of the lutein ester oil suspension of Example 1 in that: lutein ester crystal (total lutein ester content 75%) 13.5 kg, soybean oil 6 kg, glycerol dioleate 77.5 kg, vitamin E 2 kg, rosemary extract 1 kg.

[0092] The preparation method of the above lutein ester oil suspension is the same as that of Example 1.

[0093] Comparative Example 6

[0094] This comparative example provides a lutein ester oil suspension, which is the same as the raw material composition of the lutein ester oil suspension of Example 1, and the difference in the preparation method is that:

[0095] 1) Dissolve each component uniformly at 40°C to obtain composition 1;

[0096] 2) Add lutein ester crystal to composition 1, stir and dissolve at 40°C, 300 rpm for 3h, so that lutein ester is uniformly dispersed in the system to obtain composition 2;

[0097] 3) Directly shear emulsify composition 2, emulsification speed 4000 rpm, emulsification time 30 min, and finally homogenize at 350 bar pressure to obtain lutein ester oil suspension.

[0098] Comparative Example 7

[0099] This comparative example provides a lutein ester oil suspension, which is the same as the raw material composition of the lutein ester oil suspension of Example 1, and the difference in the preparation method is that:

[0100] 1) Dissolve each component uniformly at 60°C to obtain composition 1;

[0101] 2) Add lutein ester crystal to composition 1, stir and dissolve at 60°C, 300 rpm for 2.5h, so that lutein ester is uniformly dispersed in the system to obtain composition 2;

[0102] 3) Program cooling composition 2, cooling rate 25°C / h, cool to 25°C, stirring speed 100 rpm, stirring time 3h, to obtain composition 3;

[0103] 4) Shear emulsify composition 3, emulsification speed 4000 rpm, emulsification time 30 min, and finally homogenize at 350 bar pressure to obtain lutein ester oil suspension.

[0104] Comparative Example 8

[0105] Using the raw material formula and preparation method disclosed in Example 1 of Chinese patent CN202211328321.5, lutein ester oil suspension was prepared using lutein ester crystal as raw material.

[0106] Experimental Example

[0107] The lutein ester oil suspensions of each example and comparative example were evaluated for quality, as follows:

[0108] (1) Particle size: tested using an image particle analyzer (Dandong Bitai);

[0109] (2) Span PDI: PDI = (D90-D10) / D50 was calculated according to the particle size test results;

[0110] (3) Product hue: the appearance chroma of the sample was measured using a spectrophotometer by reflection method after shaking well;

[0111] (4) Application hue: taking 10% lutein ester oil suspension as an example, it was mixed and rubbed with wrapping powder according to an addition amount of 2.5‰, and the hue was detected, and the addition amount was converted when the content of the oil suspension changed;

[0112] (5) Stability: the lutein ester oil suspension was placed for 6 months during the shelf life, and the stratification was evaluated, and the stability = upper oil layer height / total height x 100%.

[0113] The results are shown in Table 1.

[0114] Table 1 Product hue value, particle size and stability results

[0115]

[0116] As can be seen from the results in Table 1, the lutein ester oil suspension prepared by the specific raw material composition and preparation method of each embodiment of the present application has small particle size, uniform distribution, good stability, and the product applied to breading powder presents golden yellow color and good coloring effect. In Comparative Example 1, the product prepared by using high content lutein ester crystals as raw material has large particle size and poor shelf stability; in Comparative Example 2, the product prepared by using low content lutein ester crystals as raw material has high crystal content, is sticky and not easy to separate, but the crystals in the system have large particle size and uneven distribution, and the PDI is also relatively large; in Comparative Example 3, the product prepared by changing the ratio of the composite emulsifier has not bright enough yellow color and poor stability; in Comparative Example 4, the oil suspension stability decreases by changing the type of emulsifier; in Comparative Example 5, the crystals precipitate in the system by using glycerol fatty acid ester as emulsifier without adding medium-chain triglyceride, and the oil suspension is seriously separated after 2 months; in Comparative Example 6, the product is directly prepared by low-temperature blending without high-temperature dissolution and programmed cooling steps, and in Comparative Example 7, the product is prepared by one-step cooling without programmed cooling, which has dark color, not bright enough yellow color in application, large particle size, uneven particle distribution, and poor stability. In Comparative Example 8, the method of patent CN202211328321.5 is used for preparation, and the lutein ester crystals precipitate, and the color is not yellow enough, which shows that the method provided in the patent is not suitable for preparing lutein ester oil suspension.

[0117] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for preparing a lutein ester oil suspension, characterized in that: The method comprises: Mixing lutein ester crystals with a first composition and heating to dissolve the crystals to obtain a second composition, wherein the first composition includes oil, a composite emulsifier, and a composite antioxidant; subjecting the second composition to programmed cooling in two stages to obtain a third composition; emulsifying and homogenizing the third composition; Wherein, the lutein ester content of the lutein ester crystals is 65%-80%; The composite emulsifier comprises medium chain triglycerides and glyceryl dioleate in a mass ratio of (60-74): (2-4); The mixing ratio of the first composition and the lutein ester crystals is: 12.5-23 parts of lutein ester crystals, 5-10 parts of oil, 62-78 parts of compound emulsifier, and 2-4 parts of compound antioxidant.

2. The method for preparing the lutein ester oil suspension according to claim 1, wherein The two-stage programmed cooling includes: The first stage of programmed cooling: cooling rate 10-20℃ / h, cooling to 25-40℃; And, the second stage programmed cooling: cooling rate 15-25℃ / h, cooling to 5-15℃.

3. The method for preparing the lutein ester oil suspension according to claim 2, wherein Stirring is performed during the programmed cooling process, wherein the stirring speed of the first stage programmed cooling is 30-50 rpm, and the stirring speed of the second stage programmed cooling is 80-120 rpm.

4. The method for preparing the lutein ester oil suspension according to claim 2, wherein The stirring time for the first stage of programmed cooling is 1-2 hours, and the stirring time for the second stage of programmed cooling is 1.5-2 hours.

5. The method for preparing the lutein ester oil suspension according to any one of claims 1 to 4, wherein: The conditions for heating and dissolving the crystals are: temperature 50-70° C., stirring speed 200-300 rpm.

6. The method for preparing the lutein ester oil suspension according to any one of claims 1 to 4, wherein: The grease is vegetable oil.

7. The method for preparing the lutein ester oil suspension according to claim 6, wherein: The oil is at least one selected from soybean oil, sunflower oil, corn oil and peanut oil.

8. The method for preparing the lutein ester oil suspension according to any one of claims 1 to 4 and 7, characterized in that: The compound antioxidant is vitamin E and rosemary extract.

9. The method for preparing the lutein ester oil suspension according to claim 8, wherein The mass ratio of vitamin E and rosemary extract is 2:(0.5-1).

10. The method for preparing the lutein ester oil suspension according to any one of claims 1 to 4, 7 and 9, characterized in that: The emulsification conditions are: emulsification rate 3000-4000 rpm, emulsification time 30-40 min; And / or, the homogenization pressure is 320-380 bar.

11. The lutein ester oil suspension prepared by the method for preparing the lutein ester oil suspension according to any one of claims 1 to 10.

12. The lutein ester oil suspension according to claim 11, characterized in that The lutein ester oil suspension has a lutein ester content of 10%-15%; and / or the lutein ester oil suspension has a particle size D50 of 8-10 μm and a span of less than 0.

5.

13. Use of the lutein ester oil suspension according to claim 11 or 12 in the preparation of food, medicine, cosmetics or feed.

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