Spartina alterniflora reconstituted tobacco and preparation method and application thereof

Abstract

The invention provides spartina alterniflora reconstituted tobacco as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) separating stems and leaves of spartina alterniflora, drying and crushing to obtain leaf powder; (2) extracting the leaf powder to obtain a leaf extracting solution; (3) mixing the leaf powder with lignin degrading bacteria, cooking, screening and pulping to obtain fiber pulp; (4) mixing the fiber pulp with the tobacco pulp to obtain pulp, and mixing the leaf extracting solution with the tobacco concentrated solution and a retention aid to obtain mixed solution; mixing the slurry and the mixed solution and making paper to obtain a reconstituted tobacco base; and (5) performing pulsed electric field treatment on the reconstituted tobacco base, and drying to obtain the spartina alterniflora reconstituted tobacco. The spartina alterniflora reconstituted tobacco provided by the invention has unique aroma components, good combustion performance, low release amount of tar and harmful components, good sensory performance and good tensile strength and dimensional stability.

Description

Technical Field

[0001] The invention belongs to the technical field of reconstituted tobacco preparation, and in particular relates to a Spartina alterniflora reconstituted tobacco leaf and a preparation method and application thereof. Background Art

[0002] With the development of the tobacco industry, the research and development and production of reconstituted tobacco have received increasing attention. Reconstituted tobacco can effectively utilize tobacco waste and other fiber raw materials, and is made into tobacco-like products through a series of processing techniques. It can partially replace natural tobacco in cigarette production, which is of great significance in reducing cigarette costs, improving cigarette quality, and reducing the impact of tobacco waste on the environment. At present, in order to improve the forming and tensile strength of reconstituted tobacco, it is necessary to add additional fibers, but the additional fibers will have an adverse effect on the sensory quality. The addition of natural plant fibers can not only improve the physical strength of reconstituted tobacco, but also reduce sensory irritation and bring a special flavor.

[0003] Spartina alterniflora is an alien plant introduced into my country. It has the characteristics of rapid growth and strong adaptability, contains more cellulose, and contains a large amount of active substances such as polysaccharides, flavonoids, saponins, etc., and has edible functions. However, there is no relevant report on the application of Spartina alterniflora in the preparation of reconstituted tobacco leaves. Therefore, how to provide a method for preparing reconstituted tobacco leaves using Spartina alterniflora has become a problem to be solved urgently. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a Spartina alterniflora reconstituted tobacco leaf and a preparation method and application thereof. The Spartina alterniflora reconstituted tobacco leaf provided by the present invention has unique aroma components, good combustion performance, low tar and harmful component release, good sensory performance, good tensile strength and dimensional stability, and meets the physical performance requirements during cigarette production and use.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a method for preparing Spartina alterniflora reconstituted tobacco leaves, the preparation method comprising the following steps:

[0007] (1) separating the stems and leaves of Spartina alterniflora, and then drying and crushing them to obtain leaf powder;

[0008] (2) extracting the leaf powder to obtain a leaf extract;

[0009] (3) mixing the leaf powder with lignin-degrading bacteria (biological pretreatment), and then steaming, screening, and beating to obtain fiber pulp;

[0010] (4) mixing the fiber pulp with the tobacco pulp to obtain a slurry, mixing the leaf extract with the tobacco concentrate and a retention aid to obtain a mixed liquid; and then mixing the slurry with the mixed liquid to make papermaking to obtain a reconstituted tobacco leaf base;

[0011] (5) The reconstituted tobacco leaf base is subjected to pulse electric field treatment, and then dried to obtain the Spartina alterniflora reconstituted tobacco leaf.

[0012] Steps (2) and (3) are performed in no particular order.

[0013] The above method extracts the stems and leaves of Spartina alterniflora and makes them into fiber pulp, and mixes them with tobacco components to make reconstituted tobacco leaves. The obtained reconstituted tobacco leaves have unique aroma components, good combustion performance, low tar and harmful component release, good sensory performance, good tensile strength and dimensional stability, and meet the physical performance requirements during cigarette production and use.

[0014] Preferably, after the separation of the stems and leaves in step (1), the process further comprises drying and crushing to obtain stem powder.

[0015] Preferably, after obtaining the stem powder, the step of extracting the stem powder to obtain a stem extract.

[0016] Preferably, the stem extract is prepared by a method comprising the following steps:

[0017] The stem powder is mixed with water, subjected to microwave extraction, and then mixed with cellulase for enzymatic hydrolysis to obtain a stem extract.

[0018] The above extraction process can effectively remove cellulose in the stem powder by using enzymatic hydrolysis, thereby improving the effective ingredients and sensory effects of the product. At the same time, the reconstituted tobacco leaves made by adding it become softer, less brittle, and less hygroscopic.

[0019] Preferably, the mass ratio of the stem powder to water is 1:(5-10), such as 1:5, 1:6, 1:7, 1:8, 1:9 or 1:10, etc., but is not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0020] Preferably, the mass of the cellulase is 0.5-1.5% of the mass of water, for example 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4% or 1.5%, etc., but is not limited to the above-listed values, and other values ​​not listed within the above numerical range are also applicable.

[0021] Preferably, the temperature of the enzymatic hydrolysis is 45-55°C, and the time is 1-3h, wherein the temperature can be 45°C, 46°C, 47°C, 48°C, 49°C, 50°C, 51°C, 52°C, 53°C, 54°C or 55°C, etc., and the time can be 1h, 1.5h, 2h, 2.5h or 3h, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0022] Preferably, the leaf extract in step (2) is prepared by a method comprising the following steps:

[0023] The leaf powder is mixed with an ethanol solution and subjected to ultrasonic extraction to obtain the leaf extract.

[0024] Preferably, the mixing in step (3) further comprises mixing with stem powder,

[0025] Preferably, the mass ratio of the stem powder to the leaf powder is 1:(1-6), such as 1:1, 1:2, 1:3, 1:4, 1:5 or 1:6, but is not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0026] Preferably, the mass of the lignin-degrading bacteria in step (3) is 5-10% of the mass of the powder, such as 5%, 6%, 7%, 8%, 9% or 10%, etc., but is not limited to the above-listed values. Other values ​​not listed within the above numerical range are also applicable.

[0027] Preferably, the mixing treatment time in step (3) is 24-48 hours, for example 24 hours, 30 hours, 35 hours, 40 hours, 45 hours or 48 hours, but is not limited to the above-listed values. Other values ​​not listed within the above-listed range are also applicable.

[0028] Preferably, the pulping in step (3) is carried out using a PFI mill.

[0029] Preferably, the pulping concentration of the beating is 2-5%, the grinding plate gap is 0.1-0.5mm, and the grinding time is 5-20min, wherein the grinding concentration can be 2%, 3%, 4% or 5%, etc., the grinding plate gap can be 0.1mm, 0.2mm, 0.3mm, 0.4mm or 0.5mm, etc., and the grinding time can be 5min, 10min, 15min or 20min, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0030] The pulping method and parameter control thereof can effectively destroy the fiber structure, release aroma components, and at the same time improve the tensile strength and dimensional stability of the product.

[0031] Preferably, the mass ratio of the fiber pulp to the tobacco pulp in step (4) is 1:(7-10), such as 1:7, 1:8, 1:9 or 1:10, but is not limited to the values ​​listed above. Other values ​​not listed within the above numerical range are also applicable.

[0032] Preferably, the mixing of the leaf extract with the tobacco concentrate and the retention aid in step (4) also includes mixing with the stem extract.

[0033] Preferably, the mass ratio of the stem extract, leaf extract and tobacco concentrate is (0-1):(1-6):(2.5-17.5), wherein the number of stem extracts can be 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1, the number of leaf extracts can be 1, 2, 3, 4, 5 or 6, the number of tobacco concentrates can be 2.5, 5, 7.5, 10, 12.5, 15 or 17.5, but are not limited to the values ​​listed above. Other values ​​not listed within the above numerical range are also applicable, and 0 means that no stem extract is added.

[0034] Preferably, the mass of the retention aid in step (4) is 5-15% of the mass of the fiber pulp, for example 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%, etc., but is not limited to the above-listed values, and other values ​​not listed within the above numerical range are also applicable.

[0035] Preferably, the retention aid in step (4) comprises any one of sodium carboxymethyl cellulose, guar gum, sodium alginate or cationic modified starch, or a combination of at least two thereof.

[0036] Preferably, the mixing to obtain the mixed liquid in step (4) further comprises mixing with a flavoring agent.

[0037] Preferably, the mass of the flavoring agent is 0.5-2% of the mass of the fiber pulp, for example 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9% or 2%, etc., but is not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0038] Preferably, the mass ratio of the slurry to the mixed liquid in step (4) is 5:(2-3), such as 5:2, 5:2.2, 5:2.4, 5:2.6, 5:2.8 or 5:3, etc., but is not limited to the values ​​listed above. Other values ​​not listed within the above numerical range are also applicable.

[0039] The above parameter control can make the Spartina alterniflora components and tobacco components organically combined, enrich the taste and quality characteristics of the product, and make the product have excellent sensory effects.

[0040] Preferably, in the pulsed electric field treatment of step (5), the electric field strength is 15-25 kV / cm, the pulse width is 20-50 μs, the pulse frequency is 50-100 Hz, and the treatment time is 10-30 min, wherein the electric field strength can be 15 kV / cm, 16 kV / cm, 17 kV / cm, 18 kV / cm, 19 kV / cm, 20 kV / cm, 21 kV / cm, 22 kV / cm, 23 kV / cm, 24 kV / cm or 25 kV / cm, etc., and the pulse The width can be 20μs, 25μs, 30μs, 35μs, 40μs, 45μs or 50μs, etc., the pulse frequency can be 50Hz, 55Hz, 60Hz, 65Hz, 70Hz, 75Hz, 80Hz, 85Hz, 90Hz, 95Hz or 100Hz, etc., and the processing time can be 10min, 15min, 20min, 25min or 30min, etc., but are not limited to the values ​​listed above. Other values ​​not listed within the above numerical range are also applicable.

[0041] The above-mentioned specific pulsed electric field treatment can optimize the product microstructure and improve physical and chemical stability.

[0042] In a second aspect, the present invention provides Spartina alterniflora reconstituted tobacco leaves prepared by the method for preparing Spartina alterniflora reconstituted tobacco leaves as described above.

[0043] In a third aspect, the present invention also provides the use of the Spartina alterniflora reconstituted tobacco leaves as described above in the preparation of reconstituted tobacco leaves.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] The invention provides a method for preparing a reconstituted tobacco leaf of Spartina alterniflora. The prepared product has rich chemical components, so that the reconstituted tobacco leaf can release unique aroma components during combustion, and is helpful to improve the combustion performance of cigarettes and reduce the release of tar and harmful components; at the same time, the product has a strong and unique aroma, a fresh herbal aroma in the top note, and rich layers of sweet aroma, fruity aroma and the like in the middle note, a coordinated and lasting aroma, a mellow and soft taste, and significantly lower irritation than that of traditional reconstituted tobacco leaves, and does not cause strong discomfort to the oral cavity and throat during smoking, and has a comfortable aftertaste, and may have good feelings such as sweetness and salivation, so as to bring consumers a high-quality smoking experience; and through a special preparation process (including biological pretreatment, PFI grinding and pulping, and pulse electric field treatment, etc.), the fiber structure of the reconstituted tobacco leaf is more uniform and compact, so that while maintaining appropriate flexibility, the product has good tensile strength and dimensional stability, and meets the physical performance requirements in the production and use of cigarettes. DETAILED DESCRIPTION

[0046] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0047] In the following examples, white rot fungi were purchased from Shanghai Collection Biotechnology Center;

[0048] Cellulase was purchased from Shandong Longkote Enzyme Preparation Co., Ltd.

[0049] Example 1

[0050] This embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is as follows:

[0051] (1) Raw material selection and processing: Collect Spartina alterniflora and separate its stems and leaves. Wash the separated leaves to remove surface impurities, and then dry them at a temperature of 50°C until the moisture content is less than 10%. Grind the dried leaves into powder with a particle size of 20-80 mesh and store them for later use.

[0052] (2) Extraction of active ingredients

[0053] Leaf extraction: Take Spartina alterniflora leaf powder, add 50% ethanol solution by volume in a mass ratio of 1:5, and perform ultrasonic extraction at 40°C for 40 minutes with an ultrasonic power of 300 W. After the extraction, filter to obtain the leaf extract, concentrate and set aside.

[0054] (3) Fiber pulp preparation

[0055] Biological pretreatment: Take Spartina alterniflora leaf powder, prepare a culture solution containing lignin-degrading bacteria (white rot fungi), and soak the mixed powder for 36 hours at an inoculation amount of 7% of the powder mass. After the soaking, filter and remove the culture solution.

[0056] Steaming treatment: Add 10% sodium hydroxide solution at a solid-liquid mass ratio of 1:5 to the powder after biological pretreatment and steam cook at 95°C for 3 hours.

[0057] Washing and screening: After cooking, multiple steps of countercurrent washing are used to remove residual alkali, lignin degradation products and other impurities. Vibrating screens are used to screen out undecomposed particles based on fiber length and coarseness distribution to obtain initially pure Spartina alterniflora fiber pulp.

[0058] PFI grinding: The fiber pulp is fed into the PFI grinding mill, and the fibers are kneaded, squeezed, and sheared through the relative movement of the grinding discs. The grinding concentration (3%), grinding disc gap (0.3mm) and grinding time (15 minutes) are precisely controlled to fully separate the fibers, improve the fiber flexibility and interweaving ability, and optimize the fiber pulp quality.

[0059] (4) Reconstituted tobacco leaf forming

[0060] The pulped Spartina alterniflora fiber pulp and tobacco pulp are mixed in a mass ratio of 1:8 to obtain a slurry, and the concentrated leaf extract and tobacco concentrate are mixed in a mass ratio of 2:10 to obtain a mixed solution, 1% of the fiber pulp mass of a flavoring agent (malic acid) and 10% of a retention aid (sodium carboxymethyl cellulose) are added, and the mixture is stirred evenly before papermaking.

[0061] Mixing system construction: According to product design requirements, the slurry and mixed liquid are accurately mixed (mass ratio 5:2.5) to build the basic structure and ensure uniform distribution of ingredients.

[0062] Papermaking and molding: Wet papermaking is adopted to form the leaf base of Spartina alterniflora reconstructed tobacco through vacuum suction and mechanical pressing. Real-time monitoring and fine-tuning of parameters are carried out to ensure quality.

[0063] Pulsed electric field treatment: Apply a pulsed electric field with an electric field strength of 20 kV / cm, a pulse width of 35 μs, and a pulse frequency of 70 Hz to the substrate for 20 minutes to optimize the microstructure and improve physical and chemical stability.

[0064] Drying treatment: drying the sheet base at 70° C. with a wind speed of 1 m / s to a moisture content of 8%-12%, thereby obtaining the Spartina alterniflora reconstituted tobacco leaves.

[0065] Example 2

[0066] This embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is as follows:

[0067] (1) Raw material selection and processing: Spartina alterniflora was collected and its stems and leaves were separated. The separated stems and leaves were cleaned separately to remove surface impurities, and then dried at a temperature of 40° C. until the moisture content was less than 10%. The dried stems and leaves were crushed into powders with a particle size of 20-80 mesh and stored separately for later use.

[0068] (2) Extraction of active ingredients

[0069] Stem extraction: Take the stem powder of Spartina alterniflora, add water in a mass ratio of 1:5, and perform microwave-assisted extraction. The microwave power is set to 300W. At the same time, add 0.5% of the mass of cellulase to the water, and perform enzymolysis at 45°C for 3 hours, then heat to 70°C and continue to extract for 2 hours. Stir continuously during the extraction process, and keep the stirring speed at 150r / min during the extraction process. After the extraction is completed, filter to obtain the stem extract, which is concentrated and set aside.

[0070] Leaf extraction: Take Spartina alterniflora leaf powder, add 60% ethanol solution by volume in a mass ratio of 1:3, and perform ultrasonic extraction at 35°C for 60 minutes with an ultrasonic power of 200 W. After the extraction, filter to obtain the leaf extract, concentrate and set aside.

[0071] (3) Fiber pulp preparation

[0072] Biological pretreatment: Mix the powder of Spartina alterniflora stem and leaf (mass ratio 1:5). Prepare a culture solution containing lignin-degrading bacteria (white rot fungi), and soak the mixed powder for 48 hours at an inoculation amount of 5% of the powder mass. After soaking, filter and remove the culture solution.

[0073] Steaming treatment: Add 8% sodium hydroxide solution at a solid-liquid mass ratio of 1:3 to the powder after biological pretreatment and steam cook at 90°C for 4 hours.

[0074] Washing and screening: After cooking, multiple steps of countercurrent washing are used to remove residual alkali, lignin degradation products and other impurities. Vibrating screens are used to screen out undecomposed particles based on fiber length and coarseness distribution to obtain initially pure Spartina alterniflora fiber pulp.

[0075] PFI grinding: The fiber pulp is fed into the PFI grinding mill, and the fibers are kneaded, squeezed, and sheared through the relative movement of the grinding discs. The grinding concentration (2%), grinding disc gap (0.1mm) and grinding time (5 minutes) are precisely controlled to fully separate the fibers, improve the fiber flexibility and interweaving ability, and optimize the fiber pulp quality.

[0076] (4) Reconstituted tobacco leaf forming

[0077] The pulped Spartina alterniflora fiber pulp and tobacco pulp are mixed in a mass ratio of 1:7 to obtain a slurry, and the concentrated stem and leaf extract and tobacco concentrate are mixed in a mass ratio of 0.1:1:2.5 to obtain a mixed solution, 0.5% of a flavoring agent (malic acid) and 5% of a retention aid (sodium carboxymethyl cellulose) accounting for the mass of the fiber pulp are added, and the mixture is stirred evenly before papermaking.

[0078] Mixing system construction: According to product design requirements, the slurry and mixed liquid are accurately mixed (mass ratio 5:2) to build the basic structure and ensure uniform distribution of ingredients.

[0079] Papermaking and molding: Wet papermaking is adopted to form the leaf base of Spartina alterniflora reconstructed tobacco through vacuum suction and mechanical pressing. Real-time monitoring and fine-tuning of parameters are carried out to ensure quality.

[0080] Pulsed electric field treatment: Apply a pulsed electric field with an electric field strength of 15kV / cm, a pulse width of 20μs, and a pulse frequency of 50Hz to the substrate for 30 minutes to optimize the microstructure and improve physical and chemical stability.

[0081] Drying treatment: drying the sheet base at 60° C. and a wind speed of 1 m / s to a moisture content of 8%-12%, thereby obtaining the Spartina alterniflora reconstituted tobacco leaves.

[0082] Example 3

[0083] This embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is as follows:

[0084] (1) Raw material selection and processing: Spartina alterniflora was collected and its stems and leaves were separated. The separated stems and leaves were cleaned separately to remove surface impurities, and then dried at a temperature of 60° C. until the moisture content was less than 10%. The dried stems and leaves were crushed into powders with a particle size of 20-80 mesh and stored separately for later use.

[0085] (2) Extraction of active ingredients

[0086] Stem extraction: Take the stem powder of Spartina alterniflora, add water in a mass ratio of 1:10, and perform microwave-assisted extraction. The microwave power is set to 500W. At the same time, add 1.5% of the mass of water to cellulase, hydrolyze at 55°C for 1 hour, then heat to 90°C, and continue to extract for 1 hour. Stir continuously during the extraction process, and keep the stirring speed at 150r / min during the extraction process. After the extraction is completed, filter to obtain the stem extract, and concentrate it for use.

[0087] Leaf extraction: Take Spartina alterniflora leaf powder, add 40% ethanol solution by volume in a mass ratio of 1:8, and perform ultrasonic extraction at 45°C for 30 minutes with an ultrasonic power of 400 W. After the extraction, filter to obtain the leaf extract, concentrate and set aside.

[0088] (3) Fiber pulp preparation

[0089] Biological pretreatment: Mix the powder of Spartina alterniflora stem and leaf (mass ratio 1:1). Prepare a culture solution containing lignin-degrading bacteria (white rot fungi), and soak the mixed powder for 24 hours at an inoculation amount of 10% of the powder mass. After soaking, filter and remove the culture solution.

[0090] Steaming treatment: Add 12% sodium hydroxide solution at a solid-liquid mass ratio of 1:6 to the powder after biological pretreatment and steam at 100°C for 2 hours.

[0091] Washing and screening: After cooking, multiple steps of countercurrent washing are used to remove residual alkali, lignin degradation products and other impurities. A centrifugal screen is used to screen out undecomposed particles based on fiber length and coarseness distribution to obtain initially pure Spartina alterniflora fiber pulp.

[0092] PFI grinding: The fiber pulp is fed into the PFI grinding mill, and the fibers are kneaded, squeezed, and sheared through the relative movement of the grinding discs. The grinding concentration (5%), grinding disc gap (0.5mm) and grinding time (20 minutes) are precisely controlled to fully separate the fibers, improve the fiber flexibility and interweaving ability, and optimize the fiber pulp quality.

[0093] (4) Reconstituted tobacco leaf forming

[0094] The pulped Spartina alterniflora fiber pulp and tobacco pulp are mixed in a mass ratio of 1:10 to obtain a slurry, and the concentrated stem and leaf extract and tobacco concentrate are mixed in a mass ratio of 1:6:17.5 to obtain a mixed solution, 2% of the fiber pulp mass of a flavoring agent (malic acid) and 15% of a retention aid (sodium carboxymethyl cellulose) are added, and the mixture is stirred evenly before papermaking.

[0095] Mixing system construction: According to product design requirements, the slurry and mixed liquid are accurately mixed (mass ratio 5:3) to build the basic structure and ensure uniform distribution of ingredients.

[0096] Papermaking and molding: Wet papermaking is adopted to form the leaf base of Spartina alterniflora reconstructed tobacco through vacuum suction and mechanical pressing. Real-time monitoring and fine-tuning of parameters are carried out to ensure quality.

[0097] Pulsed electric field treatment: A pulsed electric field with an electric field strength of 25 kV / cm, a pulse width of 50 μs, and a pulse frequency of 100 Hz is applied to the substrate for 10 minutes to optimize the microstructure and improve physical and chemical stability.

[0098] Drying treatment: drying the sheet base at 80° C. with a wind speed of 1 m / s to a moisture content of 8%-12%, thereby obtaining the Spartina alterniflora reconstituted tobacco leaves.

[0099] Example 4

[0100] This embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is the same as that of Embodiment 1 except that no cellulase is added in the stem extraction in step (2).

[0101] Example 5

[0102] This embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is the same as that of Embodiment 1 except that no lignin-degrading bacteria are added in the biological pretreatment in step (3).

[0103] Example 6

[0104] The present embodiment provides a Spartina alterniflora reconstituted tobacco leaf. The preparation method is the same as that of Embodiment 2 except that the mass ratio of the Spartina alterniflora stem and leaf powder in the biological pretreatment in step (3) is 1:0.5.

[0105] Example 7

[0106] The present embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is the same as that of Embodiment 1 except that the grinding concentration in the PFI grinding is 1%, the grinding plate gap is 0.05 mm, and the grinding time is 2 minutes in step (3).

[0107] Example 8

[0108] The present embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is the same as that of Embodiment 1 except that the grinding concentration in the PFI grinding is 7%, the grinding plate gap is 0.7 mm, and the grinding time is 25 minutes in step (3).

[0109] Example 9

[0110] The present embodiment provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is consistent with that of Embodiment 1 except that in step (4) the electric field intensity in the pulse electric field treatment is 10 kV / cm, the pulse width is 15 μs, the pulse frequency is 120 Hz, and the treatment time is 5 min.

[0111] Example 10

[0112] The present embodiment provides a reconstituted tobacco leaf of Spartina alterniflora. The preparation method is the same as that of Embodiment 1 except that in step (4), the electric field intensity in the pulse electric field treatment is 30 kV / cm, the pulse width is 60 μs, the pulse frequency is 40 Hz, and the treatment time is 25 min.

[0113] Comparative Example 1

[0114] This comparative example provides a Spartina alterniflora reconstituted tobacco leaf, the preparation method of which is the same as that of Example 1 except that PFI grinding and pulping is not performed in step (3).

[0115] Comparative Example 2

[0116] This comparative example provides a Spartina alterniflora reconstituted tobacco leaf, and the preparation method is the same as that of Example 1 except that the pulse electric field treatment is not performed in step (4).

[0117] Effect test:

[0118] The chemical composition, sensory effect and physical property tests of the Spartina alterniflora reconstituted tobacco leaves provided in Examples 1-10 and Comparative Examples 1-2 were performed, and the test methods were as follows:

[0119] The total sugar test is based on YC / T159-2019 "Continuous Flow Method for Determination of Water-soluble Sugars in Tobacco and Tobacco Products"; the total alkaloid test is based on YC / T468-2021 "Continuous Flow (Potassium Thiocyanate) Method for Determination of Total Alkaloids in Tobacco and Tobacco Products"; flavonoids and saponins are tested using high performance liquid chromatography; tensile strength test is based on GB / T 12914-2018 "Constant Rate Tensile Method for Determination of Tensile Properties of Paper and Paperboard"; softness test is based on GB / T GB / T 8942 "Determination of Paper Softness" method.

[0120] The results are as follows:

[0121] Group Total sugar / % Total plant alkaloids / % Flavonoids / % Saponin / % Example 1 10.05 1.12 1.84 1.05 Example 2 10.48 1.19 1.71 0.96 Example 3 10.97 1.21 1.67 0.91 Example 4 9.84 1.08 1.72 0.99 Example 5 10.02 1.11 1.69 1.02 Example 6 9.31 1.04 1.58 0.89 Example 7 10.01 1.07 1.79 1.00 Example 8 9.99 1.04 1.74 1.01 Example 9 9.95 1.00 1.67 1.02 Example 10 9.91 0.94 1.77 0.93 Comparative Example 1 9.95 1.00 1.67 1.02 Comparative Example 2 9.91 0.94 1.77 0.93

[0122] Group Tensile strength / N / m Softness / mN Example 1 520.52 380.83 Example 2 548.15 391.55 Example 3 533.47 478.31 Example 4 450.36 420.95 Example 5 432.14 399.22 Example 6 544.65 441.12 Example 7 497.51 457.11 Example 8 512.88 402.84 Example 9 487.34 400.67 Example 10 514.78 389.45 Comparative Example 1 475.25 423.54 Comparative Example 2 466.36 417.89

[0123] From the above data, it can be found that the Spartina alterniflora reconstituted tobacco provided by the present invention has unique aroma components, good combustion performance, low tar and harmful component release, good sensory performance, good tensile strength and dimensional stability, and meets the physical performance requirements in the production and use of cigarettes; By comparing Examples 1-10, it can be found that the present invention effectively improves the content of chemical components such as total sugar, total alkaloids, and saponins of the product by adopting a specific treatment process and controlling relevant parameters, and can release unique aroma components, with good sensory effects, high product tensile strength, and high softness; By comparing Example 1 and Comparative Examples 1-2, it can be found that the present invention effectively improves the content of active ingredients and improves the sensory effects by adopting specific pulping and pulse electric field treatment processes, and makes the fiber structure of the reconstituted tobacco more uniform and compact, so that while maintaining appropriate flexibility, it has good tensile strength and softness, and meets the physical performance requirements in the production and use of cigarettes.

[0124] The applicant declares that the present invention illustrates the Spartina alterniflora reconstituted tobacco leaves and the preparation method and application thereof through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0125] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0126] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for preparing Spartina alterniflora reconstituted tobacco, characterized in that: The preparation method comprises the following steps: (1) separating the stems and leaves of Spartina alterniflora, and then drying and crushing them to obtain leaf powder; (2) extracting the leaf powder to obtain a leaf extract; (3) mixing the leaf powder with lignin-degrading bacteria, and then steaming, screening, and beating to obtain fiber pulp; (4) mixing the fiber pulp with the tobacco pulp to obtain a slurry, mixing the leaf extract with the tobacco concentrate and a retention aid to obtain a mixed liquid; and then mixing the slurry with the mixed liquid to make papermaking to obtain a reconstituted tobacco leaf base; (5) subjecting the reconstituted tobacco leaf base to a pulse electric field treatment, and then drying to obtain the Spartina alterniflora reconstituted tobacco leaf; Steps (2) and (3) are performed in no particular order.

2. The method for preparing reconstituted tobacco leaves of Spartina alterniflora according to claim 1, characterized in that: After the separation of the stems and leaves in step (1), the step further includes drying and crushing to obtain stem powder; Preferably, after obtaining the stem powder, the step of extracting the stem powder to obtain a stem extract; Preferably, the stem extract is prepared by a method comprising the following steps: The stem powder is mixed with water, subjected to microwave extraction, and then mixed with cellulase for enzymatic hydrolysis to obtain a stem extract; Preferably, the mass ratio of the stem powder to water is 1:(5-10); Preferably, the mass of the cellulase is 0.5-1.5% of the mass of water; Preferably, the enzymatic hydrolysis temperature is 45-55°C and the time is 1-3h.

3. The method for preparing reconstituted tobacco leaves of Spartina alterniflora according to claim 1 or 2, characterized in that: The leaf extract in step (2) is prepared by a method comprising the following steps: The leaf powder is mixed with an ethanol solution and subjected to ultrasonic extraction to obtain the leaf extract.

4. The method for preparing the Spartina alterniflora reconstituted tobacco according to claim 2 or 3, characterized in that: The mixing in step (3) further comprises mixing with stem powder; Preferably, the mass ratio of the stem powder to the leaf powder is 1:(1-6); Preferably, the mass of the lignin-degrading bacteria in step (3) is 5-10% of the mass of the powder; Preferably, the mixing treatment time in step (3) is 24-48 hours.

5. The method for preparing Spartina alterniflora reconstituted tobacco according to any one of claims 1 to 4, characterized in that: The beating in step (3) is carried out by using a PFI mill; Preferably, the pulping concentration of the pulping is 2-5%, the grinding plate gap is 0.1-0.5 mm, and the pulping time is 5-20 min.

6. The method for preparing Spartina alterniflora reconstituted tobacco according to any one of claims 2 to 5, characterized in that: The mass ratio of the fiber pulp to the tobacco pulp in step (4) is 1:(7-10); Preferably, the mixing of the leaf extract with the tobacco concentrate and the retention aid in step (4) further comprises mixing with the stem extract; Preferably, the mass ratio of the stem extract, leaf extract and tobacco concentrate is (0-1):(1-6):(2.5-17.5), 0 means no stem extract is added; Preferably, the mass of the retention aid in step (4) is 5-15% of the mass of the fiber pulp; Preferably, the retention aid in step (4) comprises any one or a combination of at least two of sodium carboxymethyl cellulose, guar gum, sodium alginate or cationic modified starch; Preferably, the mixing to obtain the mixed solution in step (4) further comprises mixing with a flavoring agent; Preferably, the mass of the flavoring agent is 0.5-2% of the mass of the fiber pulp.

7. The method for preparing Spartina alterniflora reconstituted tobacco according to any one of claims 1 to 6, characterized in that: The mass ratio of the slurry to the mixed liquid in step (4) is 5:(2-3).

8. The method for preparing Spartina alterniflora reconstituted tobacco according to any one of claims 1 to 7, characterized in that: In the pulse electric field treatment of step (5), the electric field strength is 15-25 kV / cm, the pulse width is 20-50 μs, the pulse frequency is 50-100 Hz, and the treatment time is 10-30 min. 9 . A Spartina alterniflora reconstituted tobacco leaf prepared by the method for preparing Spartina alterniflora reconstituted tobacco leaf according to any one of claims 1 to 8 .

10. Use of the Spartina alterniflora reconstituted tobacco according to claim 9 in the preparation of reconstituted tobacco.