Scalp care composition and application thereof

Abstract

The invention discloses a scalp care composition and application thereof. The scalp care composition is prepared from the following raw materials in parts by weight: 0.2 to 0.4 part of cacumen biotae extract, 0.2 to 1.5 parts of rosemary leaf oil, 0.1 to 1.0 part of coffee extract and 0.1 to 1.0 part of tea leaf extract. The tea leaf extract at least comprises 55%-70% of theanine, 0.5%-2% of caffeine, 10%-20% of tea polyphenol and 10%-20% of tea polysaccharide. The composition has a good anti-hair loss effect after being compounded, and is good in anti-allergy performance, high in universality and suitable for being popularized and used in a large range.

Description

Technical Field

[0001] The invention relates to the field of daily chemicals, and in particular to a scalp care composition and application thereof. Background Art

[0002] The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.

[0003] With the accelerated pace of life and the increasing intensity of work, androgenic alopecia (seborrheic alopecia), alopecia areata and other hair loss diseases are increasing, and are showing a trend of younger people. This type of hair loss is detrimental to the appearance and beauty, and is more likely to bring huge mental stress and psychological burden to patients, which seriously affects the quality of life. In the treatment of hair loss, the commonly used Western medicines in clinical practice are minoxidil and finasteride, but both drugs are prone to relapse after discontinuation, and can also cause side effects such as skin irritation, scalp itching, facial and hand hirsutism, dizziness, anorexia, tachycardia, etc. There are many prescriptions for the treatment of hair loss recorded in Chinese medicine in my country, and there is a record in the Compendium of Materia Medica: Take Platycladus orientalis leaves, dry them in the shade and make them into powder, and apply them with oil. Compared with Western medicine ingredients, plant extract ingredients such as Chinese medicine are relatively safe and effective, with few side effects, easy to use, and milder treatment effects.

[0004] The levels of dihydrotestosterone in the scalp and serum of patients with androgenic alopecia, as well as the number and activity of 5α-reductase, are higher than those of normal people, and the ability of their hair follicles to convert testosterone into dihydrotestosterone also increases. Because 5α-reductase in hair follicles mediates the conversion of testosterone to dihydrotestosterone, the use of 5α-reductase inhibitors such as finasteride can prolong the growth period of hair follicles, prevent hair loss and promote hair growth. Further research by Guangzhou University of Chinese Medicine found that Platycladus orientalis leaf extract showed strong 5α-reductase inhibitory activity, which can reduce the conversion of testosterone to DHT and has high hair growth promoting activity in an in vivo androgen-sensitive mouse model. Rosemary, also known as tansy, was first recorded in "Supplement to Materia Medica" and is a medicinal plant that mainly contains acid phenols, flavonoids, terpenes and essential oil compounds. A large number of studies have shown that rosemary essential oil has important effects such as antibacterial, antioxidant, antidepressant, metabolic regulation and anti-inflammatory. Animal experiments have shown that rosemary extract can also inhibit 5α-reductase. However, rosemary essential oil also has many side effects. Rosemary leaf oil can also dilate blood vessels, increase blood pressure, and make people excited. Dilation of blood vessels may be the principle of minoxidil in promoting hair growth.

[0005] Caffeine is a natural purine alkaloid with good permeability. Caffeine is a phosphodiesterase (PDE) inhibitor, which increases the level of cyclic adenosine monophosphate (cAMP, also known as the "second messenger") in cells, and thus promotes cell proliferation by stimulating cell metabolism-this mechanism can counteract the miniaturization of hair follicles caused by testosterone / DHT, thereby preventing hair loss. Clinically, by adding caffeine to shampoo for subjects to use, it was found that it penetrated into the scalp two minutes after contact and stimulated hair growth. Therefore, the anti-hair loss effect can be increased by increasing the caffeine content. However, coffee penetrates through the hair follicles quickly and can penetrate into the blood, which may cause excitement of the nervous system.

[0006] Green tea contains a variety of functional ingredients. Studies have found that green tea can inhibit the production of TNF-α, and drinking large amounts of green tea will have an impact on DHT, which may be the mechanism by which green tea can treat hair loss. Another study from the Department of Dermatology at Seoul National University College of Medicine showed that epigallocatechin-3-gallate (EGCG), a component of green tea, may actually promote hair growth by stimulating dermal papilla (DPC) hair cells. According to studies conducted in vitro, EGCG caused significant elongation of hair follicles.

[0007] The prior art has provided a variety of hair growth products, such as CN111281909A discloses a hair growth composition and a preparation method thereof, comprising 12-15 parts of Forsythia suspensa, 12-15 parts of Patchouli, 10-15 parts of Gleditsia sinensis, 12-15 parts of Radix Polygalae, 12-15 parts of Platycladus orientalis, and 12-15 parts of Rosemary. The hair growth composition is a pure Chinese medicine preparation, using Platycladus orientalis and Rosemary in combination. However, the inventors of the present application found in the test of the prior art that the prior art still has an irritating effect on the scalp, which can cause allergic reactions, and has a limited hair growth effect.

[0008] There is an urgent need to improve the formula of new shampoo products. At the same time, there is still a need to enhance the anti-allergic performance of the products, improve the universality of the products, and ensure the comfort and product reputation during use by people with different skin characteristics. Summary of the invention

[0009] The purpose of the present invention is to provide a scalp care composition and its application in view of the problem that current shampoos have poor applicability to different groups of people, which can not only improve the anti-hair loss performance of the product, but also improve the universality of the product to different groups of people, improve the product's usage reputation, and thus improve the product's benefits.

[0010] The technical solution of the present invention is as follows:

[0011] In one aspect, the present invention provides a scalp care composition, comprising the following raw materials in parts by weight: 0.2-0.4 parts of Platycladus orientalis leaf extract, 0.2-1.5 parts of rosemary leaf oil, 0.1-1.0 parts of coffee extract and 0.1-1.0 parts of tea extract.

[0012] According to a preferred embodiment, the tea extract includes at least the following components in mass fractions: 55%-70% theanine, 0.5-2% caffeine, 10%-20% tea polyphenols, 10%-20% tea polysaccharides, and the balance is made up with purified water.

[0013] According to a preferred embodiment, the tea extract includes at least the following components in mass fractions: 60%-70% theanine, 0.5-1% caffeine, 15%-20% tea polyphenols, 10%-15% tea polysaccharides, and the balance is made up with purified water.

[0014] Another aspect of the present invention provides use of the scalp care composition as described above in preparing shampoo products.

[0015] Another aspect of the present invention provides a shampoo, which uses the scalp care composition as a main functional component.

[0016] Another aspect of the present invention provides a hair conditioner, which uses the scalp care composition as a main functional component.

[0017] Another aspect of the present invention provides a hair care solution, which uses the scalp care composition as described above as a main functional component.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The hair loss treatment effect is good. The ingredients of plant extracts are not easy to produce side effects. The effective combination of various ingredients can play a good anti-hair loss effect and is milder than chemical ingredients;

[0020] 2. Good universality: By adjusting the material ratio of theanine, caffeine and tea polyphenols in tea extract, the possibility of allergies in the formula is reduced, and the safety of product use and its universality for different groups of people are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is an experimental diagram of the anti-allergic effect of the composition obtained in Example 2 to Example 5 on mast cells. Compared with the blank, ###p<0.001, compared with the model, *p<0.05, **p<0.01, ***p<0.001;

[0022] Figure 2The anti-allergic effect of the composition obtained in Example 4 on mast cells, compared with the blank, ###p<0.001; compared with the model, *p<0.05, **p<0.01, ***p<0.001;

[0023] Figure 3 The figure is a result diagram of the effects of the composition of Comparative Example 2 and Example 4 and minoxidil on hair growth in mice;

[0024] Figure 4 The figure is a result diagram of the effects of the compositions of Comparative Example 2 and Example 4 and minoxidil on the hair growth length of mice. DETAILED DESCRIPTION

[0025] In this embodiment, unless otherwise specified, the preparation methods are all conventional methods, and the materials used are all commercially available.

[0026] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0027] Example 1 Preparation and extraction of tea extract

[0028] (1) Extraction of tea polyphenols and caffeine

[0029] (1.1) Dry and crush the tea leaves, pass them through a 60-80 mesh sieve to obtain tea powder, extract them under reflux at 85°C for 30 min at a ratio of tea powder to 95% ethanol in a ratio of 1:12, cool them, filter them under reduced pressure, collect the tea residues by filtration (evaporate the alcohol and set aside), and distill and concentrate the filtrate to obtain a mixture of tea polyphenols and caffeine.

[0030] (1.2) Separation of tea polyphenols

[0031] The filtrate obtained in step (1.1) is extracted with chloroform, and after sufficient extraction, the filtrate is allowed to stand for stratification, and the lower chloroform solution is released. The upper solution is fully extracted with ethyl acetate, and the obtained organic layer is concentrated and evaporated to dryness to obtain high-purity tea polyphenols.

[0032] (1.3) Separation of caffeine

[0033] The lower chloroform extract obtained in step (1.2) is concentrated by rotary evaporation, and calcium oxide is added to evaporate the chloroform, and the extract is roasted in a sand bath and sublimated at 170°C. After white crystals appear on the filter paper, heating is stopped and the extract is cooled to 100°C to obtain the first batch of caffeine. After the residue is stirred, the sand bath temperature is increased to 220°C, and the extract is sublimated again, and the caffeine obtained twice is combined.

[0034] (2) Separation of theanine

[0035] Take the aqueous phase obtained after ethyl acetate extraction in step (1.2), precipitate it with an equal volume of 95% ethanol, collect the supernatant by centrifugation, freeze-dry, dissolve the solid with a small amount of water, and then repeat crystallization with anhydrous ethanol at a low temperature of 10°C to obtain high-purity theanine.

[0036] (3) Separation of tea polysaccharides by precipitation

[0037] The tea residue obtained in step (1.1) was boiled in water for 3 times, each time for 15 minutes, filtered under reduced pressure, the filtrates were combined and concentrated, and precipitated with twice the volume of 95% ethanol to obtain a flocculent precipitate, which was filtered under reduced pressure, washed with 95% ethanol, and the precipitate was freeze-dried to obtain tea polysaccharides.

[0038] (4) Preparation of tea extract

[0039] The tea polyphenols, caffeine, theanine and tea polysaccharides prepared by the methods (1), (2) and (3) are prepared into tea extract according to the required proportions.

[0040] Embodiment 2 compounding of anti-shedding composition

[0041] The anti-hair loss composition of this application is prepared according to the following formula ingredients:

[0042] 75% ethanol 97.78%, rosemary leaf oil 1.5%, cypress leaf extract 0.4%, coffee extract 0.12%, tea extract 0.2%;

[0043] The tea extract comprises: 60% of theanine, 0.5% of caffeine, 15% of tea polyphenols and 10% of tea polysaccharides.

[0044] Embodiment 3 compounding of anti-hair loss composition

[0045] The anti-hair loss composition of this application is prepared according to the following formula ingredients:

[0046] 75% ethanol 99.4%, rosemary leaf oil 0.2%, cypress leaf extract 0.2%, coffee extract 0.1%, tea extract 0.1%;

[0047] The tea extract comprises: 60% of theanine, 0.5% of caffeine, 15% of tea polyphenols and 10% of tea polysaccharides.

[0048] Embodiment 4 compounding of anti-shedding composition

[0049] The anti-hair loss composition of this application is prepared according to the following formula ingredients:

[0050] 75% ethanol 96.1%, rosemary leaf oil 1.5%, cypress leaf extract 0.4%, coffee extract 1.0%, tea extract 1.0%;

[0051] The tea extract comprises: 60% of theanine, 0.5% of caffeine, 15% of tea polyphenols and 10% of tea polysaccharides.

[0052] Embodiment 5 compounding of anti-hair loss composition

[0053] Prepare the hair care solution of this application according to the following formula ingredients:

[0054] 75% ethanol 97.78%, rosemary leaf oil 0.65%, cypress leaf extract 0.3%, coffee extract 0.5%, tea extract 0.5%;

[0055] The tea extract comprises: 60% of theanine, 0.5% of caffeine, 15% of tea polyphenols and 10% of tea polysaccharides.

[0056] The experimental effects of the anti-shedding compositions of Examples 2 to 5 are as follows: Figure 1 and Table 1:

[0057] Anti-allergic efficacy test:

[0058] Principle of the method: The allergen DNCB can induce mast cell degranulation. The instantaneous release of mature tetrameric tryptase after mast cell degranulation will lead to a significant increase in the level of tryptase. Tryptase reacts with BAPNA to form a complex with a maximum absorption peak at 450nm. The absorbance value is proportional to the degree of allergy. The final anti-allergic effect can be calculated using the following formula.

[0059]

[0060] Method: This experiment uses 2dpf wild-type AB strain zebrafish for testing. The zebrafish are divided into groups, namely normal group, model group, positive group, and sample group using 0.1% pure water dilution of Examples 2, 3, 4, and 5 as test samples, with 10 zebrafish in each group. First, 100μg / mL BAPNA reaction solution is added to each experimental group, and then zebrafish are treated with 0.6μg / mL DNCB solution to construct a zebrafish mast cell degranulation allergy model, except for the blank control group. Then, 0.1% (10mg / mL) sodium cromoglycate is used as a positive drug to treat zebrafish in the positive group. Sodium cromoglycate is an organic compound, mainly used as an antiallergic drug, and has a good preventive and therapeutic effect on immediate allergic reactions. The sample groups are treated with 0.1% purified water dilution of Examples 2, 3, 4, and 5, respectively. After the same treatment time, the absorbance of each group is measured at a wavelength of 450nm, and the anti-allergic efficacy of each group is calculated according to the above formula. The anti-allergic effects of the compositions provided in Examples 2-5 on the zebrafish allergy model are shown in Table 1:

[0061] Table 1 Comparison of anti-allergic effects

[0062]

[0063]

[0064] From the above results, it can be seen that the ratio in Example 4 is the optimal ratio and has the best anti-allergic effect.

[0065] Embodiment 6 Compounding of anti-shedding composition

[0066] The formula of this comparative example is the same as that of Example 4 except that the composition of the tea extract is different.

[0067] The tea extract includes 62% theanine, 1.0% caffeine, 15% tea polyphenols, 10% tea polysaccharides, and the balance is made up with purified water.

[0068] Embodiment 7 Compounding of anti-hair loss composition

[0069] The formula of this comparative example is the same as that of Example 4 except that the composition of the tea extract is different.

[0070] The difference is that the tea extract includes 60% theanine, 0.8% caffeine, 20% tea polyphenols, 15% tea polysaccharides, and the balance is made up with purified water.

[0071] Embodiment 8 compounding of anti-hair loss composition

[0072] The formula of this comparative example is the same as that of Example 4 except that the composition of the tea extract is different.

[0073] The tea extract includes 70% theanine, 0.5% caffeine, 15% tea polyphenols, 10% tea polysaccharides, and the balance is made up with purified water.

[0074] Comparative Example 1

[0075] The formula of this comparative example: only the composition of the tea extract is different, and the rest is the same as Example 4. The theanine content in the tea extract of the formula of this comparative example is 40%, and the content of other substances is the same as that of Example 4, and the balance is made up with purified water. The formula of Example 4, the formula of this comparative example, and the positive drug sodium cromoglycate are used to treat allergic model mast cells, and then the tryptase content released by the mast cells is measured to evaluate the anti-allergic properties of the above samples.

[0076] The results are as follows Figure 2 As shown in Table 2, the formula obtained in Example 4 has significant anti-allergic properties, while the formula in Comparative Example 1 does not have significant anti-allergic properties, indicating that the tea extract concentration within the scope of the patent claims is necessary.

[0077] Table 2 Comparison of anti-allergic effects

[0078]

[0079] Comparative Example 2

[0080] In this comparative example, except for not adding tea extract, the total amount of the remaining substances (Platycladus orientalis leaf extract, rosemary leaf oil and coffee extract) was adjusted to be consistent with the total amount of the functional ingredients (Platycladus orientalis leaf extract, rosemary leaf oil, coffee extract and tea extract) in the composition of Example 4 (3.9%), the ratio between the remaining substances (Platycladus orientalis leaf extract, rosemary leaf oil and coffee extract) was consistent with that in Example 4, and the processing steps were completely consistent with those in Example 4.

[0081] Animal experiments were conducted using the composition of comparative example 2, the composition obtained in Example 4 containing tea extract, and the chemical drug minoxidil to test their efficacy in treating hair loss. A total of 18 SPF-grade adult C57BL / 6J mice were selected and randomly divided into 3 groups according to gender, with 6 mice in each group, half of which were male and half were female. They were 6 weeks old and weighed (20±2) g. The back area of ​​the mice was shaved using a shaver, and the hair removal area was 2cm×3cm. The composition of comparative example 2, the composition prepared in Example 4, and the positive drug 2% minoxidil were evenly applied to the exposed skin on the back of the mice, with an application amount of 1 mL, twice a day, for 21 consecutive days, and the effects of promoting hair growth and preventing hair loss were observed. The test results are as follows: Figure 3 and Figure 4 As shown, the composition of Example 4 effectively inhibits hair loss and promotes hair growth in mice, and its effect is stronger than minoxidil. The effect of the composition of Comparative Example 2 on inhibiting hair loss and promoting hair growth in mice is worse than that of the composition of Example 4.

[0082] The above-mentioned embodiments only express the specific implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the technical solution concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A scalp care composition, characterized in that: The invention comprises the following raw materials in parts by weight: 0.2-0.4 parts of Platycladus orientalis leaf extract, 0.2-1.5 parts of rosemary leaf oil, 0.1-1.0 parts of coffee extract and 0.1-1.0 parts of tea extract.

2. A scalp care composition according to claim 1, characterized in that: The tea extract at least comprises the following components in mass fractions: 55%-70% of theanine, 0.5-2% of caffeine, 10%-20% of tea polyphenols and 10%-20% of tea polysaccharides.

3. A scalp care composition according to claim 1, characterized in that: The tea extract at least comprises the following components in mass fractions: 60%-70% of theanine, 0.5%-1% of caffeine, 15%-20% of tea polyphenols and 10%-15% of tea polysaccharides.

4. Use of the scalp care composition according to claim 1 in the preparation of anti-hair loss shampoo products.

5. Use of a scalp care composition according to claim 4 in the preparation of an anti-hair loss shampoo product, wherein the anti-hair loss shampoo product comprises a shampoo, a conditioner or a hair conditioner.

6. A shampoo comprising a scalp care composition according to any one of claims 1 to 3 as a main functional ingredient.

7. A hair conditioner, comprising a scalp care composition according to any one of claims 1 to 3 as a main functional ingredient.

8. A hair care liquid, comprising a scalp care composition according to any one of claims 1 to 3 as a main functional ingredient.

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