Composition with hair-strengthening, hair-loss-preventing, hair-growing and hair-blackening effects and application thereof

Abstract

The invention relates to a composition with hair-strengthening, hair-loss-preventing, hair-growing and hair-blackening effects and application thereof. The composition comprises a pea seedling extract, an apple extract, a lycium ruthenicum extract and nine-time steamed and nine-time sun-dried rhizoma polygonati. Wherein the pea seedling extract, the apple extract, the lycium ruthenicum extract and the nine-time steamed and nine-time sun-dried rhizoma polygonati can interact and positively influence one another, hair regeneration and new hair improvement are promoted, the alopecia phenomenon is improved, and meanwhile the effect of promoting early white hair to turn black is achieved. The composition provided by the invention adopts pure natural plant extracts and pure traditional Chinese medicine preparations as raw materials, is green and safe, and can jointly maintain the health and growth of hair from multiple angles.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine products, and relates to a composition with the effects of strengthening hair, preventing hair loss, promoting hair growth and darkening hair, and application thereof. Background Art

[0002] Hair is the hair that grows on the human head and is an appendage of the skin. The growth of hair originates from the hair follicles, and the periodic activity of the hair follicles determines the growth pattern of hair. Under normal circumstances, the hair follicles in the scalp cycle through three stages: growth phase, regression phase, and resting phase. In the three phases of hair growth, the dermal papilla cells are the first protagonist, which affects the development and growth of hair follicles, controls the transformation of hair from resting phase to growth phase, and induces the proliferation, upward migration, and differentiation of the surrounding epithelial cells, thus ultimately forming the structure of the hair.

[0003] The normal growth cycle of hair depends on the self-renewal of hair follicles. When the dynamic balance of the three stages of growth, degeneration and rest is broken, it will lead to excessive hair loss. Hair loss is mainly divided into physiological and pathological types. Physiological hair loss belongs to normal metabolism, and a small amount of hair loss every day is a normal phenomenon; while pathological hair loss refers to abnormally large amounts of hair loss, including androgenic alopecia, alopecia areata and telogen effluvium. According to traditional Chinese medicine theory, hair is closely related to the liver and kidneys. The kidneys store essence and the liver governs blood, and its beauty is in the hair. If the liver and kidneys are deficient, the essence and blood are insufficient, and the hair follicles cannot get enough nutrition. In one case, the ability to synthesize melanin is weakened, resulting in white hair, and in another case, the hair follicles atrophy or necrosis, causing hair loss. On the contrary, if the liver and kidneys are strong and nourish the head, the hair will be thick and black.

[0004] The occurrence of gray hair is related to factors such as heredity, mental stress, excessive use of the brain, and malnutrition. Traditional Chinese medicine believes that gray hair is related to liver and kidney deficiency and blood imbalance; modern medical research shows that the growth, color, quantity, and dryness of hair are closely related to hair nutrient deficiency. Pathological gray hair is mainly caused by reduced melanin formation in hair, which is caused by the weakening of the function of melanocytes to form melanin and the reduced activity of tyrosinase. There are roughly the following situations: deficiency of essence and weak blood, excessive blood heat, and liver depression and spleen dampness. Currently, the existing products for treating hair loss and dark hair are mostly added with chemical ingredients and hormone components, which are easy to relapse after stopping use, and the effect is not ideal. Therefore, it is of great significance to develop a green, natural, mild, and easy-to-use hair growth and dark hair product. Summary of the invention

[0005] In view of the deficiencies in the prior art, the object of the present invention is to provide a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, the composition comprising pea seedling extract, apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum.

[0008] Pea sprout extract is a natural ingredient extracted from pea sprouts. It can stimulate the two major hair growth factors FGF7 and Noggi, activate hair papilla cells, and awaken dormant hair follicles. It mainly promotes the proliferation activity of hair keratinocytes, shortens the hair resting period and promotes hair growth at the beginning of a new hair growth period. The nutrients in apples can induce the production of keratin in hair follicles, significantly increase the expression of fibroblast factors and vascular endothelial growth factors, and inhibit the expression of related genes that affect the normal metabolism of hair follicle cells, effectively improving hair density, increasing hair weight and reducing the amount of hair loss. In addition to the extremely high content of anthocyanins, black wolfberry also contains high levels of vitamin C, iron and zinc, which can nourish yin and kidney, promote blood circulation, improve sleep, create a natural and healthy environment for hair growth, and effectively improve hair loss caused by kidney deficiency. Polygonatum has the effects of tonifying the middle and replenishing qi, tonifying the kidney and replenishing essence, and can alleviate hair loss and premature graying of hair caused by kidney essence and yin deficiency. Polygonatum after nine steaming and nine drying can reduce the greasy and sticky nature of the drug, making the drug easier to digest, and can decompose the toxicity and impurities in the drug, making the medicinal properties purer and more peaceful.

[0009] In the present invention, after the above four components are used in combination, it is found that they have significant synergistic effects in terms of hair health prevention and hair growth.

[0010] Preferably, the composition comprises, by weight, 1-10 parts of pea seedling extract, 0.5-8 parts of apple extract, 4-12 parts of black wolfberry extract and 3-15 parts of nine-steamed and nine-dried polygonatum;

[0011] The above “1-10 parts” may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.;

[0012] The above-mentioned “0.5-8 parts” can be, for example, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 6 parts, 7 parts, 8 parts, etc.;

[0013] The above-mentioned “4-12 parts” can be, for example, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, etc.;

[0014] The above-mentioned "3-15 parts" can be, for example, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc. Other specific point values ​​within the numerical range can be selected, and they will not be elaborated here one by one.

[0015] In the present invention, when the dosage ratio of each component in the composition is within the above specific range, it has more excellent hair strengthening, hair loss prevention and hair growth and darkening effects.

[0016] The pea sprout extract involved in the present invention is not limited to its specific preparation process. When it is prepared by the following process, its synergistic effect with the other three components in hair health prevention and hair growth and darkening is more significant.

[0017] Preferably, the pea sprout extract is prepared by a preparation method comprising the following steps:

[0018] The pea seedlings are freeze-dried and ground, then mixed with a complex enzyme solution, subjected to microwave and ultrasonic co-extraction treatment, the treated solution is centrifuged, the precipitate is mixed with water, and the microwave and ultrasonic co-extraction process is repeated, centrifuged, the supernatant is collected and combined, and freeze-dried to obtain the pea seedling extract;

[0019] The enzymes in the complex enzyme solution include beta-glucosidase, pectinase, bromelain and ficin.

[0020] In the present invention, it is unexpectedly found that when a composite enzyme solution containing four enzymes, namely, β-glucosidase, pectinase, bromelain and ficin, is used to perform enzymatic hydrolysis and extraction of active substances in pea seedlings, the combination of the four enzymes can synergistically enhance the effect and obtain a better extraction effect. The prepared pea seedling extract has high activity and excellent anti-hair loss and blackening effects. In addition, in the present invention, microwave and ultrasound are used in combination to assist enzymatic hydrolysis, and the synergistic treatment of the two can further improve the enzymatic hydrolysis effect and obtain a pea seedling extract with a better effect.

[0021] Preferably, the mass ratio of the β-glucosidase, pectinase, bromelain and ficin is (1-5):(2-7):(0.1-2):(0.05-2);

[0022] The above “1-5” may be, for example, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.;

[0023] The above-mentioned “2-7” may be, for example, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, etc.;

[0024] The above-mentioned “0.1-2” can be, for example, 0.1, 0.2, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, etc.;

[0025] The above-mentioned "0.05-2" can be, for example, 0.05, 0.1, 0.2, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, etc. Other specific point values ​​within this numerical range can be selected, and they will not be elaborated here one by one.

[0026] In the present invention, when the dosage ratio of the four enzymes in the composite enzyme is within the above-mentioned specific range, the enzymatic extraction effect on pea seedlings can be further improved.

[0027] Preferably, the addition amount of the complex enzyme in the complex enzyme solution is 0.1‰-0.5‰ of the mass of pea seedlings, for example, it can be 0.1‰, 0.15‰, 0.2‰, 0.25‰, 0.3‰, 0.35‰, 0.4‰, 0.45‰, 0.5‰, etc. Other specific point values ​​within this numerical range can be selected, and they will not be repeated here.

[0028] Preferably, the ratio of the pea seedlings to the complex enzyme solution is 1g:(30-80)mL, for example, it can be 1g:30mL, 1g:35mL, 1g:40mL, 1g:45mL, 1g:50mL, 1g:55mL, 1g:60mL, 1g:65mL, 1g:70mL, 1g:72mL, 1g:75mL, 1g:80mL, etc. Other specific point values ​​within this numerical range can be selected, and they will not be repeated here one by one.

[0029] Preferably, the power of the microwave in the co-extraction treatment is 200-800 W, for example 200 W, 300 W, 400 W, 500 W, 600 W, 700 W, 800 W, etc., and the microwave time is 10-60 s, for example 10 s, 20 s, 30 s, 40 s, 50 s, 60 s, etc.; the power of the ultrasonic wave is 200-400 W, for example 200 W, 250 W, 300 W, 350 W, 400 W, etc., and the ultrasonic time is 5-30 min, for example 5 min, 7 min, 10 min, 12 min, 15 min, 20 min, 22 min, 25 min, 30 min, etc.

[0030] The apple extract and black wolfberry extract involved in the present invention are not limited to their specific preparation processes. When they are prepared using the following processes respectively, their synergistic effects with the other three components in hair health, hair loss prevention, hair growth and hair darkening are more significant.

[0031] Preferably, the apple extract or black wolfberry extract is prepared by a preparation method comprising the following steps:

[0032] The apple raw material or the black wolfberry raw material is crushed and sieved, mixed with water, subjected to microfluidic extraction treatment, centrifuged to obtain the supernatant, concentrated and dried to obtain the product.

[0033] When microfluidization extraction is used to extract apple raw materials or black wolfberry raw materials, the extraction effect can be further improved. Compared with conventional heating reflux extraction, the nutritional active substances in apples or black wolfberry can be more effectively extracted, and the obtained extract has a better effect of strengthening hair and preventing hair loss.

[0034] Preferably, the pressure of the microfluidic extraction is 20-300 MPa, for example, it can be 20 MPa, 50 MPa, 75 MPa, 100 MPa, 125 MPa, 150 MPa, 180 MPa, 200 MPa, 240 MPa, 260 MPa, 280 MPa, 300 MPa, etc.; the temperature is 20-50°C, for example, it can be 20°C, 22°C, 25°C, 28°C, 30°C, 32°C, 35°C, 38°C, 40°C, 42°C, 45°C, 48°C, 50°C, etc.; the number of material cycles is 2-10 times, for example, it can be 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, 9 times, 10 times.

[0035] Preferably, the composition further comprises any one of rice germ, mulberry, Poria cocos, Gastrodia elata, Angelica sinensis or yeast, or a combination of at least two thereof.

[0036] Preferably, the composition further comprises, by weight, any one of 0.5-10 parts of rice germ, 5-10 parts of mulberry, 5-10 parts of Poria cocos, 2-8 parts of Gastrodia elata, 1-6 parts of Angelica sinensis, or 0.5-4 parts of yeast, or a combination of at least two thereof;

[0037] The above “0.5-10 parts” can be, for example, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.;

[0038] The above-mentioned “5-10 parts” can be, for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.;

[0039] The above-mentioned “2-8 parts” can be, for example, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 6 parts, 7 parts, 8 parts, etc.;

[0040] The above-mentioned “1-6 parts” can be, for example, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 6 parts, etc.;

[0041] The above-mentioned "0.5-4 parts" can be, for example, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described one by one here.

[0042] Preferably, the composition further comprises a flavoring agent and / or a filler.

[0043] Preferably, the composition further comprises 4-8 parts of flavoring agent and / or 20-45 parts of filler, by weight;

[0044] The above-mentioned “4-8 parts” may be, for example, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.;

[0045] The above-mentioned "20-45 parts" can be, for example, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, etc. Other specific point values ​​within the numerical range can be selected, and they will not be elaborated here one by one.

[0046] Preferably, the flavoring agent includes any one of erythritol, xylitol, sorbitol, maltitol, steviol glycosides, mogrosides, sucralose, citric acid or sodium citrate, or a combination of at least two thereof.

[0047] Preferably, the filler comprises any one of resistant dextrin, inulin, maltodextrin, isomaltooligosaccharide, polydextrose or oligofructose, or a combination of at least two thereof.

[0048] In a second aspect, the present invention provides a use of the composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to the first aspect in the preparation of hair care and nourishing products.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] In the present invention, four specific ingredients, namely, pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum, are used in combination. Among them, pea sprout extract can promote the proliferation activity of hair keratinocytes, shorten the hair resting period and promote hair growth; the nutrients in apples can induce the production of keratin in hair follicles; black wolfberry and steamed and dried polygonatum can nourish yin and kidney, replenish the middle and benefit qi, and effectively alleviate and improve the phenomena of hair loss, premature graying of hair and the like caused by kidney deficiency. The combined use of the four components can synergistically enhance the effect and obtain better hair health, hair loss prevention, hair growth and hair darkening effects. DETAILED DESCRIPTION

[0051] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0052] The β-glucosidase involved in the following examples was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., model G755171; pectinase was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., model P755168; bromelain was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., model B755342; fig proteinase was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., model F755315; nine-steamed and nine-dried Polygonatum sibiricum powder was purchased from Shanghai Shidianyi Health Technology Co., Ltd.; commercially available pea sprout extract, commercially available apple extract and black wolfberry extract were all purchased from Shaanxi Snott Biotechnology Co., Ltd.; concentrated rice germ powder was purchased from Shanghai Dewei Biotechnology Co., Ltd.; yeast powder was yeast powder rich in B vitamins, purchased from Shanyi Food (Shanghai) Co., Ltd.

[0053] Preparation Example 1

[0054] This preparation example provides a pea sprout extract, which is prepared by the following method:

[0055] (1) freeze-dried and ground pea seedlings and then mixed with a composite enzyme solution, wherein the amount of composite enzyme added to the composite enzyme solution is 0.25‰ of the mass of the pea seedlings, and the solid-liquid ratio is 1 g:55 mL, wherein the enzymes in the composite enzyme solution are composed of β-glucosidase, pectinase, bromelain and ficin in a mass ratio of 3:5:1:1, and microwave ultrasonic co-extraction treatment is performed at a constant temperature of 55°C, the microwave power is 450W, and the microwave time is 30s; the ultrasonic power is 300W, and the ultrasonic time is 20min;

[0056] (2) The treated liquid was centrifuged at 4000 rpm for 15 min, the supernatant was collected, the precipitate was mixed with water, and the solid-liquid ratio was 1 g:55 mL. The co-extraction process of step (1) and the centrifugation process of step (2) were repeated once, the supernatant was collected and combined, and freeze-dried to obtain a pea seedling extract.

[0057] This preparation example also provides an apple extract and a black wolfberry extract prepared by the following method:

[0058] The apple raw material and the black wolfberry raw material were crushed and sieved respectively, and then mixed with water at a solid-liquid ratio of 1g:10mL and subjected to microfluidic extraction treatment. The extraction pressure was 150MPa, the temperature was 30°C, and the number of solid-liquid cycles was 5 times. The treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the apple extract and the black wolfberry extract.

[0059] Preparation Example 2

[0060] This preparation example provides a pea sprout extract, which is prepared by the following method:

[0061] (1) freeze-dried and ground pea seedlings and then mixed with a composite enzyme solution, wherein the amount of composite enzyme added to the composite enzyme solution is 0.1‰ of the mass of the pea seedlings, and the solid-liquid ratio is 1 g:70 mL, wherein the enzymes in the composite enzyme solution are composed of β-glucosidase, pectinase, bromelain and ficin in a mass ratio of 2:6:0.5:1.5, and microwave ultrasonic co-extraction treatment is performed at a constant temperature of 65° C., the microwave power is 300 W, and the microwave time is 50 s; the ultrasonic power is 200 W, and the ultrasonic time is 30 min;

[0062] (2) The treated liquid was centrifuged at 4000 rpm for 15 min, the supernatant was collected, the precipitate was mixed with water, and the solid-liquid ratio was 1 g:70 mL. The co-extraction process of step (1) and the centrifugation process of step (2) were repeated once, and the supernatant was collected and combined, and freeze-dried to obtain a pea seedling extract.

[0063] This preparation example also provides an apple extract and a black wolfberry extract prepared by the following method:

[0064] The apple raw material and the black wolfberry raw material were crushed and sieved respectively, and then mixed with water at a solid-liquid ratio of 1g:15mL and subjected to microfluidic extraction treatment. The extraction pressure was 50MPa, the temperature was 45°C, and the number of solid-liquid cycles was 3 times. The treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the apple extract and the black wolfberry extract.

[0065] Preparation Example 3

[0066] This preparation example provides a pea sprout extract, which is prepared by the following method:

[0067] (1) freeze-dried and ground pea seedlings and then mixed with a composite enzyme solution, wherein the amount of composite enzyme added to the composite enzyme solution is 0.4‰ of the mass of the pea seedlings, and the solid-liquid ratio is 1 g:40 mL, wherein the enzymes in the composite enzyme solution are composed of β-glucosidase, pectinase, bromelain and ficin in a mass ratio of 4:3:1.8:2, and microwave ultrasonic co-extraction treatment is performed at a constant temperature of 60°C, the microwave power is 600 W, and the microwave time is 20 s; the ultrasonic power is 350 W, and the ultrasonic time is 15 min;

[0068] (2) The treated liquid was centrifuged at 4000 rpm for 15 min, the supernatant was collected, the precipitate was mixed with water, and the solid-liquid ratio was 1 g:40 mL. The co-extraction process of step (1) and the centrifugation process of step (2) were repeated once, the supernatant was collected and combined, and freeze-dried to obtain a pea seedling extract.

[0069] This preparation example also provides an apple extract and a black wolfberry extract prepared by the following method:

[0070] The apple raw material and the black wolfberry raw material were crushed and sieved respectively, and then mixed with water at a solid-liquid ratio of 1g:12mL and subjected to microfluidic extraction treatment. The extraction pressure was 260MPa, the temperature was 25°C, and the number of solid-liquid cycles was 9 times. The treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the apple extract and the black wolfberry extract.

[0071] Preparation Example 4

[0072] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts in Preparation Example 1. The preparation method thereof is different from that in Preparation Example 1 only in that the enzyme composition in the complex enzyme is different, that is, β-glucosidase is not contained, and its reduced portion is proportionally distributed to the three enzymes of pectinase, bromelain and ficin. Other processing processes and related parameters are referred to Preparation Example 1.

[0073] Preparation Example 5

[0074] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts in Preparation Example 1. The preparation method is different from that in Preparation Example 1 only in the enzyme composition in the complex enzyme, that is, pectinase is not contained, and the reduced portion is proportionally distributed to the three enzymes of β-glucosidase, bromelain and ficin. Other processing processes and related parameters are referred to Preparation Example 1.

[0075] Preparation Example 6

[0076] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts in Preparation Example 1. The preparation method is different from that in Preparation Example 1 only in that the enzyme composition in the complex enzyme is different, that is, bromelain is not included, and the reduced portion is proportionally distributed to the three enzymes of β-glucosidase, pectinase and ficin. Other processing processes and related parameters are referred to Preparation Example 1.

[0077] Preparation Example 7

[0078] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts in Preparation Example 1. The preparation method thereof is different from that in Preparation Example 1 only in that the enzyme composition in the complex enzyme is different, that is, it does not contain ficin, and its reduced portion is proportionally distributed to the three enzymes of β-glucosidase, pectinase and bromelain. Other processing processes and related parameters are referred to Preparation Example 1.

[0079] Preparation Example 8

[0080] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts and the complex enzyme solution in Preparation Example 1. The preparation method is different from that in Preparation Example 1 only in that the extraction process in step (1) only uses microwave treatment, the microwave power is 550 W, and the time is 50 s; then the obtained treatment solution is subjected to the subsequent step (2) to obtain the pea sprout extract.

[0081] Preparation Example 9

[0082] This preparation example provides a pea sprout extract, which uses the freeze-dried and ground pea sprouts and the complex enzyme solution in Preparation Example 1. The preparation method is different from that in Preparation Example 1 only in that in the extraction process in step (1), only ultrasonic treatment is used, and the microwave power is 400 W and the time is 25 min; then the prepared treated liquid is subjected to subsequent (2) to obtain a pea sprout extract.

[0083] Preparation Example 10

[0084] This preparation example provides an apple extract, which is prepared by the following method:

[0085] The apple raw material was crushed and sieved, mixed with water at a solid-liquid ratio of 1g:12mL, heated under reflux for extraction for 3h, and the filtrate after extraction was filtered with filter paper, concentrated and freeze-dried to obtain the apple extract.

[0086] Preparation Example 11

[0087] This preparation example provides a black wolfberry extract, which is prepared by the following method:

[0088] The black wolfberry raw material was crushed and sieved, mixed with water at a solid-liquid ratio of 1g:12mL, heated under reflux for extraction for 3h, and the filtrate after extraction was filtered with filter paper, concentrated and freeze-dried to obtain the black wolfberry extract.

[0089] Example 1

[0090] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 5 parts of pea seedling extract (Preparation Example 1), 4 parts of apple extract (Preparation Example 1), 8 parts of black wolfberry extract (Preparation Example 1) and 9 parts of nine-steamed and nine-dried polygonatum powder.

[0091] Example 2

[0092] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 2 parts of pea sprout extract (Preparation Example 2), 7 parts of apple extract (Preparation Example 2), 5 parts of black wolfberry extract (Preparation Example 2) and 12 parts of nine-steamed and nine-dried polygonatum powder.

[0093] Example 3

[0094] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 8 parts of pea seedling extract (Preparation Example 3), 1 part of apple extract (Preparation Example 3), 12 parts of black wolfberry extract (Preparation Example 3) and 5 parts of nine-steamed and nine-dried polygonatum powder.

[0095] Example 4

[0096] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 4).

[0097] Example 5

[0098] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 5).

[0099] Example 6

[0100] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 6).

[0101] Example 7

[0102] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 7).

[0103] Example 8

[0104] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 8).

[0105] Example 9

[0106] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between this composition and Example 1 is that the pea sprout extract (Preparation Example 1) is replaced with an equal amount of pea sprout extract (Preparation Example 9).

[0107] Example 10

[0108] This embodiment provides a composition having the effects of strengthening hair and preventing hair loss as well as promoting hair growth and darkening hair. The difference between this composition and Embodiment 1 is that the apple extract (Preparation Example 1) is replaced by an equal amount of apple extract (Preparation Example 10).

[0109] Embodiment 11

[0110] This embodiment provides a composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between this composition and Example 1 is that the black wolfberry extract (Preparation Example 1) used is replaced with an equal amount of black wolfberry extract (Preparation Example 11).

[0111] Example 12

[0112] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 5 parts of pea seedling extract (commercially available), 4 parts of apple extract (commercially available), 8 parts of black wolfberry extract (commercially available) and 9 parts of nine-steamed and nine-dried polygonatum powder.

[0113] Example 13

[0114] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 5 parts of pea seedling extract (Preparation Example 1), 4 parts of apple extract (Preparation Example 1), 8 parts of black wolfberry extract (Preparation Example 1), 9 parts of nine-steamed and nine-dried polygonatum powder, 6 parts of rice germ powder, 6 parts of mulberry powder, 5 parts of poria powder, 4 parts of gastrodia powder, 3 parts of angelica powder, 2.5 parts of yeast powder rich in B vitamins, 6 parts of resistant dextrin and 41.5 parts of erythritol.

[0115] Embodiment 14

[0116] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 2 parts of pea seedling extract (Preparation Example 2), 7 parts of apple extract (Preparation Example 2), 5 parts of black wolfberry extract (Preparation Example 2), 12 parts of nine-steamed and nine-dried polygonatum powder, 2 parts of rice germ powder, 9 parts of mulberry powder, 8 parts of Poria powder, 2 parts of Gastrodia powder, 1 part of Angelica powder, 4 parts of yeast powder rich in B vitamins, 4 parts of inulin and 24 parts of xylitol.

[0117] Embodiment 15

[0118] The present embodiment provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair, which is composed, by mass, of 8 parts of pea seedling extract (Preparation Example 3), 1 part of apple extract (Preparation Example 3), 12 parts of black wolfberry extract (Preparation Example 3), 5 parts of nine-steamed and nine-dried polygonatum powder, 8 parts of rice germ powder, 5 parts of mulberry powder, 7 parts of Poria powder, 7 parts of Gastrodia powder, 5 parts of Angelica powder, 1 part of yeast powder rich in B vitamins, 7.5 parts of maltodextrin and 33.5 parts of maltitol.

[0119] Comparative Example 1

[0120] This comparative example provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between it and Example 1 is that it does not contain pea sprout extract, and the reduced amount of pea sprout extract is proportionally distributed to the three components of apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum powder.

[0121] Comparative Example 2

[0122] This comparative example provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between it and Example 1 is that it does not contain apple extract, and the reduced amount of apple extract is proportionally distributed to the three components of pea sprout extract, black wolfberry extract and nine-steamed and nine-dried polygonatum powder.

[0123] Comparative Example 3

[0124] This comparative example provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between it and Example 1 is that it does not contain black wolfberry extract, and the reduced amount of black wolfberry extract is proportionally distributed to the three components of pea sprout extract, apple extract and nine-steamed and nine-dried polygonatum powder.

[0125] Comparative Example 4

[0126] This comparative example provides a composition with the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair. The only difference between it and Example 1 is that it does not contain nine-steamed and nine-dried polygonatum powder, and its reduced amount is proportionally distributed to the three components of apple extract, black wolfberry extract and pea sprout extract.

[0127] Test Example 1

[0128] Animal hair growth promotion experiment

[0129] (1) Test samples: The raw materials of each component in Examples 1-15 and Comparative Examples 1-4 were weighed according to the corresponding amount, placed in a mixer and fully mixed for 15 minutes before testing.

[0130] (2) Animal treatment and grouping: The experimental animals were female C57BL / 6 mice aged 45-48 days, weighing 20-22 g. During the experiment, each group of mice was kept in a quiet environment with (12±2) hours of light during the day, an indoor temperature of (20±2)°C, and a relative humidity of 40% to 60%. The mice were fed with ordinary commercially available feed (in accordance with "GB 14924.3-2001 Laboratory Animal Mice and Rats Formula Feed") and had free access to food and water. The drinking water and feed were changed daily, and the living environment of the animals was kept ventilated and clean. After one week of adaptive feeding, a mixture of rosin and paraffin with a mass ratio of 1:1 was heated and melted and applied to the back of the mice under mild anesthesia. After it solidified and hardened, it was gently peeled off. The hair removal area of ​​each mouse was 2×2 cm 2 .

[0131] Grouping: The mice after depilation were weighed and randomly divided into 20 groups, 5 mice in each group. Mice of different weights were evenly distributed in each group, and each group was free to eat and drink. Drinking water and feed were changed every day. The samples to be tested in Examples 1-15 and Comparative Examples 1-4 treated in step (1) were gavaged once a day at a fixed time, and the gavage dose was 1 g / kg.BW. The blank group was gavaged with physiological saline of equal mass. The gavage was continued for 21 days. On the 22nd day, the gross weight of the hair condition of the mice in the new hair area was measured, the average was taken, and the ratio of the hair condition before and after the experiment was compared. The results are shown in Table 1.

[0132] Table 1

[0133]

[0134]

[0135] From the data in the table, we can see that:

[0136] (1) It can be seen from Examples 1 to 3 that the present invention uses pea sprout extract, apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum for combined use, which can cooperate with each other, synergistically enhance the effect, and jointly promote hair growth.

[0137] (2) By comparing Example 1 with Examples 4-7, it can be seen that the pea sprout extract in the present invention is enzymatically extracted by a composite enzyme consisting of four enzymes: β-glucosidase, pectinase, bromelain and ficin. The four enzymes can synergistically promote the enzymatic hydrolysis effect after being used in combination, further improving the extraction effect of the relevant active ingredients in the pea sprouts, and obtaining a pea sprout extract with better effect.

[0138] (3) By comparing Example 1 with Examples 8-9, it can be seen that the combined treatment of microwave and ultrasound in the present invention can further enhance the enzymatic hydrolysis effect and produce a pea sprout extract with higher activity.

[0139] (4) By comparing Example 1 with Examples 10-11, it can be seen that the black wolfberry extract and apple extract prepared by the microfluidic extraction method in the present invention have better effects than the corresponding extracts prepared by traditional heating reflux extraction.

[0140] (5) By comparing Example 1 with Example 12, it can be seen that the pea sprout extract, apple extract and black wolfberry extract prepared by the specific process of the present invention have a further better effect of promoting hair growth.

[0141] (6) By comparing Examples 1-3 with Examples 13-15, it can be seen that after the rice germ, mulberry, Poria cocos, Gastrodia elata, Angelica sinensis, and yeast components are additionally added in the present invention, the obtained composition has a better effect of promoting hair growth.

[0142] (7) By comparing Example 1 with Comparative Examples 1-4, it can be seen that when Comparative Examples 1-4 do not contain any of the four components of pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum, the effect of the prepared composition on promoting hair growth is worse than that of Example 1. It can be seen that the four components of pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum used in the present invention can cooperate with each other, synergistically enhance the effect, and jointly promote hair growth.

[0143] Test Example 2

[0144] Human hair loss prevention and hair growth test

[0145] Test sample: Test sample: After weighing the raw materials of each component in Examples 1-15 and Comparative Examples 1-4 according to the corresponding amount, place them in a mixer and mix them thoroughly for 15 minutes before testing.

[0146] Test subjects and groups: 200 people aged 20-45 years old with hair loss, hair loss and hair breakage were selected. A control design was adopted. According to the requirements of randomization and double-blind, voluntary subjects who met the inclusion criteria and guaranteed to cooperate with the test were grouped as much as possible considering factors affecting the test results such as age, gender, and hair loss conditions. Each group had 10 people. The test group took the samples to be tested of Examples 1-15 and Comparative Examples 1-4 after treatment in step (1), once a day, 2 g / time. The control group took a placebo for 60 consecutive days. During the test period, the original eating habits were not changed and the diet was normal.

[0147] The efficacy evaluation criteria are shown in the following table:

[0148]

[0149] Effective rate = (number of effective patients / 10) × 100%; Effective rate (%) = [(number of effective patients + number of effective patients) / 10] × 100%. The test results are shown in Table 2.

[0150] Table 2

[0151] Number of people with significant effect Effective number of people Invalid number of people Efficiency(%) Effectiveness (%) Example 1 8 2 0 80 100 Example 2 7 2 1 70 90 Example 3 8 1 1 80 90 Example 4 7 1 2 70 80 Example 5 6 2 2 60 80 Example 6 7 0 3 70 70 Example 7 6 3 1 60 90 Example 8 7 1 2 70 80 Example 9 5 2 3 50 70 Example 10 5 1 4 50 60 Embodiment 11 6 0 4 60 60 Example 12 4 2 4 40 60 Example 13 10 0 0 100 100 Embodiment 14 9 1 0 90 100 Embodiment 15 10 0 0 100 100 Comparative Example 1 3 2 5 30 50 Comparative Example 2 3 1 6 30 40 Comparative Example 3 2 2 6 20 40 Comparative Example 4 3 2 5 30 50 Control group (placebo) 0 0 10 0 0

[0152] From the data in the table, we can see that:

[0153] (1) It can be seen from Examples 1 to 3 that the four components of the present invention, namely, pea sprout extract, apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum, are used in combination, which can cooperate with each other, synergistically enhance the effect, and jointly improve the hair loss situation and promote hair growth.

[0154] (2) By comparing Example 1 with Examples 4-7, it can be seen that the pea sprout extract in the present invention is enzymatically extracted by a composite enzyme consisting of four enzymes: β-glucosidase, pectinase, bromelain and ficin. The four enzymes can synergistically promote the enzymatic hydrolysis effect after being used in combination, further improving the extraction effect of the relevant active ingredients in the pea sprouts, and obtaining a pea sprout extract with better effect.

[0155] (3) By comparing Example 1 with Examples 8-9, it can be seen that the combined treatment of microwave and ultrasound in the present invention can further enhance the enzymatic hydrolysis effect and produce a pea sprout extract with higher activity.

[0156] (4) By comparing Example 1 with Examples 10-11, it can be seen that the black wolfberry extract and apple extract prepared by the microfluidic extraction method in the present invention have better effects than the corresponding extracts prepared by traditional heating reflux extraction.

[0157] (5) By comparing Example 1 with Example 12, it can be seen that the pea sprout extract, apple extract and black wolfberry extract prepared by the specific process of the present invention have a further better effect in promoting the improvement of hair loss.

[0158] (6) By comparing Examples 1-3 with Examples 13-15, it can be seen that after the rice germ, mulberry, Poria cocos, Gastrodia elata, Angelica sinensis, and yeast components are additionally added in the present invention, the resulting composition has a better effect of improving hair loss and promoting hair growth.

[0159] (7) By comparing Example 1 with Comparative Examples 1-4, it can be seen that when Comparative Examples 1-4 do not contain any of the four components of pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum, the effect of the prepared composition on promoting hair growth is worse than that of Example 1. It can be seen that the four components of pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum used in the present invention can cooperate with each other, synergistically enhance the effect, and jointly improve hair loss and promote hair growth.

[0160] Test Example 3

[0161] Hair darkening effect test

[0162] Test sample: Test sample: After weighing the raw materials of each component in Examples 1-15 and Comparative Examples 1-4 according to the corresponding amount, place them in a mixer and mix them thoroughly for 15 minutes before testing.

[0163] Test subjects and groups: 200 people aged 20-45 with mild gray hair (gray hair rate within 50%) were selected. A control design was adopted. According to the requirements of randomization and double-blind, voluntary subjects who met the inclusion criteria and guaranteed to cooperate with the test were grouped as much as possible considering factors affecting the test results such as age, gender, and hair loss conditions. Each group had 10 people. The test group took the samples to be tested of Examples 1-15 and Comparative Examples 1-4 after treatment in step (1), once a day, 2 g / time. The control group took a placebo for 60 consecutive days. During the test period, the original eating habits were not changed and the diet was normal.

[0164] The efficacy evaluation criteria are: effective: the number of white hairs decreases and the number of black hairs increases; ineffective: no significant changes. The test results are shown in Table 3.

[0165] Table 3

[0166] Effectiveness (%) Example 1 80 Example 2 90 Example 3 80 Example 4 70 Example 5 60 Example 6 70 Example 7 50 Example 8 50 Example 9 60 Example 10 50 Embodiment 11 40 Example 12 40 Example 13 100 Embodiment 14 100 Embodiment 15 90 Comparative Example 1 30 Comparative Example 2 30 Comparative Example 3 20 Comparative Example 4 20 Blank control group 0

[0167] It can be seen from the data in the table that the combination of pea sprout extract, apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum provided by the present invention has a significant hair blackening effect, which can effectively promote the transformation of white hair into black, and after additionally adding rice germ, mulberry, Poria cocos, Gastrodia elata, Angelica sinensis and yeast components, the hair blackening effect of the composition product can be further enhanced.

[0168] In summary, in the present invention, four specific ingredients, namely, pea sprout extract, apple extract, black wolfberry extract and steamed and dried polygonatum, are used in combination. Among them, pea sprout extract can promote the proliferation activity of hair keratinocytes, shorten the hair resting period and promote hair growth; the nutrients in apples can induce the production of keratin in hair follicles; black wolfberry and steamed and dried polygonatum can nourish yin and kidney, replenish the middle and benefit qi, and effectively alleviate and improve the phenomena of hair loss, premature graying of hair and the like caused by kidney deficiency. The combined use of the four components can synergistically enhance the effect and obtain better hair health, hair loss prevention, hair growth and hair darkening effects.

[0169] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0170] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0171] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition having the effects of strengthening hair, preventing hair loss and promoting hair growth, characterized in that: The composition comprises pea sprout extract, apple extract, black wolfberry extract and nine-steamed and nine-dried polygonatum.

2. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 1, characterized in that: The composition comprises, by weight, 1-10 parts of pea seedling extract, 0.5-8 parts of apple extract, 4-12 parts of black wolfberry extract and 3-15 parts of nine-steamed and nine-dried polygonatum.

3. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 1 or 2, characterized in that: The pea seedling extract is prepared by a preparation method comprising the following steps: The pea seedlings are freeze-dried and ground, then mixed with a complex enzyme solution, subjected to microwave and ultrasonic co-extraction treatment, the treated solution is centrifuged, the precipitate is mixed with water, and the microwave and ultrasonic co-extraction process is repeated, centrifuged, the supernatant is collected and combined, and freeze-dried to obtain the pea seedling extract; The enzymes in the complex enzyme solution include beta-glucosidase, pectinase, bromelain and ficin.

4. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 3, characterized in that: The mass ratio of the β-glucosidase, pectinase, bromelain and ficin is (1-5):(2-7):(0.1-2):(0.05-2).

5. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 3, characterized in that: The amount of the compound enzyme added to the compound enzyme solution is 0.1‰-0.5‰ of the mass of the pea seedlings; Preferably, the ratio of the pea seedlings to the complex enzyme solution is 1 g: (30-80) mL.

6. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 1 or 2, characterized in that: The apple extract or black wolfberry extract is prepared by a preparation method comprising the following steps: The apple raw material or the black wolfberry raw material is crushed and sieved, mixed with water, subjected to microfluidic extraction treatment, centrifuged to obtain the supernatant, concentrated and dried to obtain the product.

7. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 6, characterized in that: The pressure of the microfluidic extraction is 20-300 MPa, the temperature is 20-50° C., and the number of material cycles is 2-10 times.

8. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to any one of claims 1 to 7, characterized in that: The composition further comprises any one of rice germ, mulberry, Poria cocos, Gastrodia elata, Angelica sinensis or yeast, or a combination of at least two thereof.

9. The composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to claim 8, characterized in that: In parts by mass, the composition further comprises any one of 0.5-10 parts of rice germ, 5-10 parts of mulberry, 5-10 parts of Poria cocos, 2-8 parts of Gastrodia elata, 1-6 parts of Angelica sinensis or 0.5-4 parts of yeast, or a combination of at least two of them.

10. Use of the composition having the effects of strengthening hair, preventing hair loss and promoting hair growth and darkening hair according to any one of claims 1 to 9 in the preparation of hair care and nourishing products.

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