Xanthophyll eye protection composition and application thereof

Abstract

The invention provides a lutein eye protection composition and application thereof. The composition is prepared from the following raw materials in parts by weight: 1 to 40 parts of lutein or lutein ester, 1 to 8 parts of zeaxanthin, 1 to 500 parts of corn oligopeptide, 1 to 500 parts of semen cassiae extract, 1 to 500 parts of flos chrysanthemi extract, 1 to 250 parts of liquorice root extract and 1 to 500 parts of anthocyanin. The composition disclosed by the invention can effectively protect human retinal pigment epithelial cells ARPE-19 from being damaged by blue light, so that the effects of relieving asthenopia and protecting eyesight are achieved; in addition, the oxidative damage of human retinal pigment epithelial cells ARPE-19 can be obviously relieved, and the effect of protecting eyesight is achieved. The raw materials of the composition cooperate with each other and supplement each other, so that the eye protection composition can effectively relieve visual fatigue for a long time and protect eyesight.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine and food, and specifically relates to a lutein eye protection composition and application thereof. Background Art

[0002] In recent years, with the changes in the social environment, the popularization of video terminals and the accelerated pace of work, visual use has been far overloaded, and more and more people are experiencing symptoms of visual fatigue such as dry eyes, swelling and pain, and blurred vision. The main symptoms of visual fatigue include eye discomfort, dryness, pain, soreness, and intolerance to long-term vision. These symptoms not only affect the patient's visual quality, but may also interfere with daily life and work efficiency, thereby reducing the quality of life. Epidemiological research results show that 23% of school-age children, 64%-90% of computer users, and 71.3% of dry eye patients have varying degrees of visual fatigue symptoms.

[0003] At present, the main ways to relieve visual fatigue are: using eye drops, hot compresses on the eyes, eye exercises, etc. The use of eye drops will directly contact the cornea, and some people may experience discomfort. Although hot compresses on the eyes, eye exercises, etc. can also relieve visual fatigue, they can only provide temporary relief in general and are not effective for a long time. Summary of the invention

[0004] Based on this, the object of the present invention is to provide a lutein eye protection composition and its application in the preparation of eye protection products, wherein the eye protection composition can effectively relieve visual fatigue and protect eyesight for a long time.

[0005] To achieve the above objectives, the present invention adopts the following technical solutions.

[0006] The first aspect of the present invention provides a composition for relieving visual fatigue and protecting eyesight, wherein the composition comprises the following raw materials in parts by weight: 1-40 parts of lutein or lutein ester, 1-8 parts of zeaxanthin, 1-500 parts of corn oligopeptides, 1-500 parts of cassia seed extract, 1-500 parts of chrysanthemum extract, 1-250 parts of licorice extract, and 1-500 parts of anthocyanins.

[0007] In some embodiments, the composition comprises the following raw materials in parts by weight: 5-40 parts of lutein or lutein ester, 2-8 parts of zeaxanthin, 100-500 parts of corn oligopeptides, 100-500 parts of cassia seed extract, 100-500 parts of chrysanthemum extract, 20-250 parts of licorice extract, and 100-500 parts of anthocyanins.

[0008] In some embodiments, the composition comprises the following raw materials in parts by weight: 20-40 parts of lutein or lutein ester, 4-8 parts of zeaxanthin, 200-500 parts of corn oligopeptides, 200-500 parts of cassia seed extract, 200-500 parts of chrysanthemum extract, 50-200 parts of licorice extract, and 200-500 parts of anthocyanins.

[0009] In some embodiments, the composition further comprises at least one of the following raw materials in parts by weight: 1-100 parts of astragalus extract and 1-100 parts of radish seed extract; preferably, the composition further comprises at least one of the following raw materials in parts by weight: 10-100 parts of astragalus extract and 10-100 parts of radish seed extract.

[0010] In some embodiments, the ratio of the total weight of lutein or lutein ester to zeaxanthin, the total weight of corn oligopeptides, the total weight of cassia seed extract, chrysanthemum extract, liquorice extract, astragalus extract and radish seed extract is (32-40): (350-450): (1350-1450), preferably (35-40): (350-400): (1350-1400).

[0011] In some embodiments, the composition further comprises the following raw materials in parts by weight: 1-100 parts of ursolic acid; preferably, the composition further comprises the following raw materials in parts by weight: 10-100 parts of ursolic acid.

[0012] The second aspect of the present invention provides the use of the above-mentioned composition in the preparation of a product for relieving visual fatigue and protecting eyesight.

[0013] In some embodiments, the product is a pharmaceutical.

[0014] The third aspect of the present invention provides a product for relieving visual fatigue and protecting eyesight, the product comprising an active ingredient and a food or pharmaceutically acceptable adjuvant; the active ingredient comprises the composition as described above.

[0015] In some embodiments, the food or pharmaceutically acceptable excipients include at least one of a sweetener, a sour agent, an anticaking agent and a glidant.

[0016] In some embodiments, the sweetener is steviol glycoside; and / or the acidulant is citric acid; and / or the anti-caking agent is magnesium stearate; and / or the glidant is silicon dioxide.

[0017] In some embodiments, the product comprises the following components: the composition, 0.01 to 1 part of the sweetener, 0.01 to 1 part of the sour agent, 0.01 to 1 part of the anti-caking agent and 0.01 to 1 part of the glidant.

[0018] In some embodiments, the product is in the form of tablets, capsules, granules, powders, solutions, powders, pills, emulsions or suspensions.

[0019] The present invention provides a composition that can effectively relieve visual fatigue and protect vision for a long time, and the composition comprises lutein or lutein ester, zeaxanthin, corn oligopeptide, cassia seed extract, chrysanthemum extract, liquorice extract and anthocyanin in a suitable ratio. By compounding raw materials with eye protection effect with a specific Chinese medicine composition, the survival rate of human retinal pigment epithelial cells under blue light irradiation can be effectively improved, and the oxidative damage of human retinal pigment epithelial cells ARPE-19 can be significantly reduced, the peroxidation state of eye tissue can be effectively relieved, visual fatigue can be relieved, and vision can be protected.

[0020] Furthermore, the inventors have found through research that the composition formed by further adding an appropriate amount of astragalus extract and / or radish seed extract to the above raw materials can further enhance the eye protection effect. In particular, when astragalus extract and radish seed extract are added at the same time, an unexpected synergistic effect is produced between the components, which can greatly improve the effect of the composition in relieving visual fatigue and protecting vision. In addition, the total weight of lutein or lutein ester and zeaxanthin, the weight of corn oligopeptides, the total weight of cassia seed extract, chrysanthemum extract, licorice extract, astragalus extract and radish seed extract in the composition has a significant effect on the eye protection effect of the composition. When the ratio is (32-40): (350-450): (1350-1450), the composition can have a better eye protection effect.

[0021] In addition, adding ursolic acid having a liver-protecting effect into the composition can further enhance the eye-protecting effect of the composition, better relieve visual fatigue, and protect vision. DETAILED DESCRIPTION

[0022] The preferred embodiment of the present invention will be described in detail below in conjunction with specific embodiments. It should be noted that the following specific embodiments are provided only for the purpose of illustration and are not intended to limit the scope of the present invention. Those skilled in the art may make various modifications and substitutions to the present invention without departing from the purpose and spirit of the present invention.

[0023] The experimental methods used in the following specific examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following specific examples can be obtained from conventional commercial channels or prepared according to conventional methods in the art unless otherwise specified.

[0024] One embodiment of the present invention relates to a composition for relieving visual fatigue and protecting eyesight, wherein the composition comprises the following raw materials in parts by weight: 1-40 parts of lutein or lutein ester, 1-8 parts of zeaxanthin, 1-500 parts of corn oligopeptides, 1-500 parts of cassia seed extract, 1-500 parts of chrysanthemum extract, 1-250 parts of licorice extract, and 1-500 parts of anthocyanins.

[0025] The composition obtained by compounding raw materials with eye protection effects with a specific traditional Chinese medicine composition can effectively improve the survival rate of human retinal pigment epithelial cells under blue light irradiation, and significantly reduce the oxidative damage of human retinal pigment epithelial cells ARPE-19, effectively alleviate the peroxidation state of eye tissue, relieve visual fatigue, and protect vision.

[0026] Lutein / Lutein Ester: Lutein is an important carotenoid, mainly found in the macula of the human eye. It can protect the retina, filter blue light, and has an antioxidant effect. Lutein relieves eye fatigue by promoting blood circulation in the eyes and improving the nutrient supply to the eyes. Lutein ester is a form of lutein that can be converted into free lutein under photochemical action. Lutein ester can not only effectively supplement lutein, but also improve its absorption efficiency in the eyes, thereby better relieving visual fatigue.

[0027] Zeaxanthin is a natural carotenoid with multiple functions such as anti-oxidation, blue light filtering and retinal protection. Therefore, it shows significant effects in relieving visual fatigue.

[0028] Corn oligopeptides have a protective effect on alcoholic liver damage and acute liver damage induced by carbon tetrachloride in mice, and can reduce oxidative stress in hepatocytes, thereby protecting the liver.

[0029] Cassia seed extract can improve the antioxidant capacity of eye tissue and reduce the level of lipid peroxides in the tissue, thereby effectively alleviating the peroxidation state of eye tissue and helping to reduce visual fatigue.

[0030] Chrysanthemum extract has the effects of clearing the liver and improving eyesight, clearing away heat and detoxifying, and can treat symptoms such as headaches, dizziness, red and painful eyes, etc.

[0031] Licorice extract has a certain protective effect on the liver. The liver is closely related to the eyes. Traditional Chinese medicine emphasizes that the liver can improve eyesight, and eye diseases can be treated by regulating liver function.

[0032] Anthocyanins have strong antioxidant capacity, which can remove free radicals and reduce the damage of oxidative stress to the eyes. At the same time, anthocyanins can also promote the regeneration of rhodopsin and relax the ciliary muscles, thereby reducing eye muscle fatigue.

[0033] In some embodiments, the composition comprises the following raw materials in parts by weight: 1-40 parts of lutein or lutein ester, 1-8 parts of zeaxanthin, 1-500 parts of corn oligopeptides, 1-500 parts of cassia seed extract, 1-500 parts of chrysanthemum extract, 1-250 parts of licorice extract, 1-500 parts of anthocyanins, 1-100 parts of astragalus extract, and 1-100 parts of radish seed extract.

[0034] The astragalus extract can replenish qi and strengthen the exterior, and when combined with other raw materials in the composition, it has a certain effect on the maintenance of the eyes.

[0035] Radish seed extract can promote blood circulation in the eyes, have a positive regulatory effect on the lens of the eye, and thus relieve visual fatigue.

[0036] The inventors have found through research that by further adding appropriate amounts of astragalus extract and radish seed extract to the above raw materials to form a composition, unexpected synergistic effects are produced between the components, which can greatly improve the effect of the composition in relieving visual fatigue and protecting vision.

[0037] Furthermore, the ratio of the total weight of lutein or lutein ester and zeaxanthin, the weight of corn oligopeptides, the total weight of cassia seed extract, chrysanthemum extract, liquorice extract, astragalus extract and radish seed extract in the composition has a significant effect on the eye protection effect of the composition. When the ratio is (32-40): (350-450): (1350-1450), the composition can have a better eye protection effect, preferably (35-40): (350-400): (1350-1400).

[0038] In some embodiments, the composition comprises the following raw materials in parts by weight: 1-40 parts of lutein or lutein ester, 1-8 parts of zeaxanthin, 1-500 parts of corn oligopeptides, 1-500 parts of cassia seed extract, 1-500 parts of chrysanthemum extract, 1-250 parts of licorice extract, 1-500 parts of anthocyanins, 1-100 parts of astragalus extract, 1-100 parts of radish seed extract, and 1-100 parts of ursolic acid. Adding ursolic acid, which has a liver-protecting effect, to the composition can further enhance the eye-protecting effect of the composition, better relieve visual fatigue, and protect vision.

[0039] The preparation method of the composition of the present invention comprises the step of mixing the components.

[0040] In some embodiments, the preparation method of the composition comprises the following steps: S1: weighing each raw material according to the weight ratio; S2: putting the weighed materials into the equipment, stirring and mixing them thoroughly for 20-25 minutes to mix them evenly.

[0041] The composition of the present invention can be prepared with relevant edible auxiliary materials through conventional production processes to prepare products for relieving visual fatigue and protecting eyesight, including medicines and foods, etc. The products can be eaten for a long time, are safe and effective, have a suitable taste, are widely accepted, and have good development and application prospects.

[0042] One embodiment of the present invention relates to a product for relieving visual fatigue and protecting eyesight, wherein the product comprises an active ingredient and excipients acceptable to food or pharmacology; the active ingredient comprises the composition as described above.

[0043] In some embodiments, the dosage form of the product is tablets, capsules (including soft capsules), granules, powders, and solutions.

[0044] The following is a description with reference to specific embodiments.

[0045] In the present invention, the Cassia seed extract, chrysanthemum extract, liquorice extract, astragalus extract and radish seed extract are water extracts and / or alcohol extracts, which can be obtained by purchasing commercial products or by using conventional water extraction or alcohol extraction methods in the art. For example, the extracts are obtained by the following extraction method:

[0046] Cassia seed extract: Cassia seeds are crushed and sieved, and 5 to 15 times the weight of the raw material is added with water or a certain degree of ethanol to extract 1 to 3 times, the extracts are combined, concentrated to a paste, and then dried to obtain the extract.

[0047] Chrysanthemum extract: After the chrysanthemum is crushed and sieved, water 15 to 25 times the weight of the raw material or ethanol of a certain degree is added to extract 1 to 3 times, the extracts are combined, concentrated to a paste, and then dried to obtain the extract.

[0048] Licorice extract: Grind the licorice and sieve it, add 25 to 35 times the weight of the raw material water or a certain degree of ethanol to extract 1 to 3 times, combine the extracts, concentrate to a paste, and then dry it to obtain the extract.

[0049] Astragalus extract: After the astragalus is crushed, it is sieved and extracted 1 to 3 times with water 25 to 35 times the weight of the raw material or ethanol of a certain degree. The extracts are combined, concentrated to a paste, and then dried to obtain the extract.

[0050] Radish seed extract: The radish seeds are crushed and sieved, and 5 to 15 times the weight of the raw material is added with water or ethanol of a certain degree to extract 1 to 3 times, the extracts are combined, concentrated to a paste, and then dried to obtain the extract.

[0051] Preferably, the extracts used in the following examples are prepared by the following method:

[0052] Cassia seed extract: Cassia seeds are crushed and sieved, extracted three times with distilled water 10 times the weight of the raw material, the water extracts are combined, concentrated to a paste, and then freeze-dried to obtain the extract.

[0053] Chrysanthemum extract: The chrysanthemum is crushed and sieved, and extracted three times with distilled water 20 times the weight of the raw material. The water extracts are combined, concentrated to a paste, and then freeze-dried to obtain the extract.

[0054] Licorice extract: Licorice is crushed and sieved, extracted three times with distilled water 30 times the weight of the raw material, the water extracts are combined, concentrated to a paste, and then freeze-dried to obtain the extract.

[0055] Astragalus extract: Astragalus is crushed and sieved, extracted three times with distilled water 30 times the weight of the raw material, the water extracts are combined, concentrated to a paste, and then freeze-dried to obtain the extract.

[0056] Radish seed extract: The radish seeds are crushed and sieved, and extracted three times with distilled water 10 times the weight of the raw material. The water extracts are combined, concentrated to a paste, and then freeze-dried to obtain the extract.

[0057] Example 1

[0058] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 1 part of lutein, 1 part of zeaxanthin, 100 parts of corn oligopeptides, 100 parts of cassia seed extract, 100 parts of chrysanthemum extract, 50 parts of licorice extract, 10 parts of astragalus extract, 10 parts of radish seed extract, 100 parts of anthocyanins, and 10 parts of ursolic acid.

[0059] The preparation method of the composition comprises the following steps:

[0060] S1: Weigh the above raw materials according to the weight ratio.

[0061] S2: Put the weighed materials into the equipment and stir them thoroughly for 25 minutes until they are evenly mixed.

[0062] S3: The stirred material is quantitatively packaged by an automatic filling machine.

[0063] Example 2

[0064] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, 50 parts of astragalus extract, 50 parts of radish seed extract, 250 parts of anthocyanins, and 50 parts of ursolic acid.

[0065] The preparation method of the composition is the same as that of Example 1.

[0066] Example 3

[0067] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 30 parts of lutein, 6 parts of zeaxanthin, 400 parts of corn oligopeptides, 500 parts of cassia seed extract, 500 parts of chrysanthemum extract, 200 parts of licorice extract, 100 parts of astragalus extract, 100 parts of radish seed extract, 500 parts of anthocyanins, and 100 parts of ursolic acid.

[0068] The preparation method of the composition is the same as that of Example 1.

[0069] Example 4

[0070] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which comprises the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, and 250 parts of anthocyanins.

[0071] The preparation method of the composition is the same as that of Example 1.

[0072] Example 5

[0073] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, 50 parts of astragalus extract, 50 parts of radish seed extract, and 250 parts of anthocyanins.

[0074] The preparation method of the composition is the same as that of Example 1.

[0075] Example 6

[0076] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, 100 parts of radish seed extract, and 250 parts of anthocyanins.

[0077] The preparation method of the composition is the same as that of Example 1.

[0078] Example 7

[0079] The present embodiment provides a composition for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, 100 parts of astragalus extract, and 250 parts of anthocyanins.

[0080] The preparation method of the composition is the same as that of Example 1.

[0081] Example 8

[0082] The present embodiment provides a drug for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 1 part of lutein ester, 1 part of zeaxanthin, 100 parts of corn oligopeptides, 100 parts of cassia seed extract, 100 parts of chrysanthemum extract, 50 parts of licorice extract, 10 parts of astragalus extract, 10 parts of radish seed extract, 100 parts of anthocyanins, 10 parts of ursolic acid, 0.06 parts of steviol glycosides, 0.04 parts of citric acid, 0.4 parts of magnesium stearate, and 0.2 parts of silicon dioxide.

[0083] The preparation method of the drug comprises the following steps:

[0084] S1: Weigh the above raw materials according to the weight ratio.

[0085] S2: Put the weighed materials into the equipment and stir them thoroughly for 25 minutes until they are evenly mixed.

[0086] S3: The stirred material is quantitatively packaged by an automatic filling machine to obtain a medicine having the effects of improving visual fatigue and protecting eyes.

[0087] Example 9

[0088] The present embodiment provides a drug for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 10 parts of lutein, 10 parts of lutein ester, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of cassia seed extract, 250 parts of chrysanthemum extract, 125 parts of licorice extract, 50 parts of astragalus extract, 50 parts of radish seed extract, 250 parts of anthocyanins, 50 parts of ursolic acid, 0.1 parts of steviol glycosides, 0.08 parts of citric acid, 0.6 parts of magnesium stearate, and 0.4 parts of silicon dioxide.

[0089] The preparation method of the drug is the same as that of Example 8.

[0090] Example 10

[0091] The present embodiment provides a drug for relieving visual fatigue and protecting eyes, which includes the following raw materials in parts by weight: 15 parts of lutein, 15 parts of lutein ester, 6 parts of zeaxanthin, 400 parts of corn oligopeptides, 500 parts of cassia seed extract, 500 parts of chrysanthemum extract, 200 parts of licorice extract, 100 parts of astragalus extract, 100 parts of radish seed extract, 500 parts of anthocyanins, 100 parts of ursolic acid, 0.04 parts of steviol glycosides, 0.02 parts of citric acid, 0.3 parts of magnesium stearate, and 0.1 parts of silicon dioxide.

[0092] The preparation method of the drug is the same as that of Example 8.

[0093] Comparative Example 1

[0094] This comparative example provides a composition for relieving visual fatigue and protecting eyes. The composition is the same as Example 4 except that the Cassia seed extract replaces an equal amount of blueberry powder. Specifically, the composition includes the following raw materials in parts by weight: 20 parts of lutein, 4 parts of zeaxanthin, 250 parts of corn oligopeptides, 250 parts of blueberry powder, 250 parts of chrysanthemum extract, 125 parts of licorice extract, and 250 parts of anthocyanins.

[0095] The preparation method of the composition is the same as that of Example 1.

[0096] Example 11 Cell Efficacy Verification Test

[0097] This experimental example tests the effects of the compositions prepared in the above examples 1-7 and comparative example 1.

[0098] The cell culture medium was DMEM high-glucose medium containing 10% fetal bovine serum and 1% commercial antibiotics, wherein DMEM high-glucose medium was purchased from Beijing Solebow Technology Co., Ltd., and fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd. CCK8 reagent was purchased from Beijing Solebow Technology Co., Ltd., and malondialdehyde (MDA), glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) kits were purchased from Nanjing Jiancheng Bioengineering Research Institute Co., Ltd.

[0099] 1. Effect on cell viability

[0100] The test cells are human retinal pigment epithelial cells ARPE-19, and the cell experiments are as follows:

[0101] ARPE-19 cells were seeded in 96-well plates at 5 × 10 3Cells were cultured in a cell culture incubator at 37°C and 5% CO2 overnight with 100uL of culture medium per well. After discarding the culture medium, complete culture medium was added to the blank group and the model group. 100uL of the product solution of Examples 1-7 and Comparative Example 1 diluted with new culture medium was added to each test group (the concentrations of the product solutions of Examples 1-7 and Comparative Example 1 were both 40ug / mL). After culturing in a cell culture incubator at 37°C and 5% CO2 for 24h, the culture medium was discarded and complete culture medium was added. ARPE-19 cells (except the blank group) were irradiated under blue light with an illumination intensity of 4000-5333Lux for 2h. 10uL of CCK8 was added to each well. After incubation for 1h, the OD value was detected at a wavelength of 450nm on a microplate reader.

[0102] Table 1 shows the results of the effects on the cell viability of human retinal pigment epithelial cells ARPE-19 in each test group.

[0103] Table 1

[0104]

[0105]

[0106] As shown in Table 1, compared with the blank group, the cell survival rate of the model group (blue light irradiation) was significantly reduced. Compared with the model group, each test group significantly increased the cell survival rate. Among them, the cell survival rate of the composition treatment group of Examples 1 to 3 was higher.

[0107] Among the treatment groups of the compositions of Examples 1 to 3, the cell survival rate of the treatment group of the composition of Example 3 was the highest. The inventors have found through research that the ratio of the total weight of lutein or lutein ester and zeaxanthin, the weight of corn oligopeptides, the total weight of cassia seed extract, chrysanthemum extract, liquorice extract, astragalus extract and radish seed extract in the composition will affect the synergistic effect between the raw materials, and the preferred ratio is (32-40): (350-450): (1350-1450), and more preferably (35-40): (350-400): (1350-1400). Compared with Examples 1 and 2, the raw material dosage ratio of Example 3 is 36:400:1400, and the raw materials produce better synergistic effect, which can more effectively reduce the blue light damage of human retinal pigment epithelial cells ARPE-19.

[0108] Compared with Example 4, radish seed extract was added to the composition of Example 6, and astragalus extract was added to the composition of Example 7, and the survival rate of cells treated with the composition was improved. Compared with Examples 6 and 7, astragalus extract and radish seed extract were added to the composition of Example 5 at the same time, and the survival rate of cells treated with the composition was greatly improved. The results show that the composition formed by adding an appropriate amount of astragalus extract and / or radish seed extract to the composition of Example 4 can further reduce the blue light damage of human retinal pigment epithelial cells ARPE-19. In particular, the simultaneous addition of astragalus extract and radish seed extract (Example 5) produces an unexpected synergistic effect between the components, which can greatly reduce the blue light damage of human retinal pigment epithelial cells ARPE-19.

[0109] Compared with Example 5, the addition of ursolic acid to the composition of Example 2 further improved the cell survival rate, indicating that the addition of ursolic acid to the composition of the present invention can further improve the effect of the composition.

[0110] Compared with Example 4, the composition of Comparative Example 1 replaced the Cassia seed extract with an equal amount of blueberry powder with eye protection effect, but the cell survival rate was significantly reduced.

[0111] 2. Assessment of antioxidant capacity at the cellular level

[0112] The test cells are human retinal pigment epithelial cells ARPE-19, and the cell experiments are as follows:

[0113] ARPE-19 cells were seeded in 6-well plates at 5 × 10 5 Cells, 2mL culture medium per well, cultured overnight in a 37°C, 5% CO2 cell culture incubator, discarded the culture medium, the blank group and the model group added complete culture medium, each test group added 2mL of the product solution of Example 1-7 and Comparative Example 1 diluted with new culture medium, (the concentration of the product solution of Example 1-7 and Comparative Example 1 is 40ug / mL), 37°C, 5% CO2 cell culture incubator culture for 24h, discarded the culture medium, washed twice with PBS, added complete culture medium, ARPE-19 cells (except the blank group) were placed under blue light with an illumination intensity of 4000-5333Lux for 2h, discarded the culture medium, washed twice with PBS, scraped the cells with a cell scraper, ultrasonically disrupted, obtained the cell disruption solution and measured the content of malondialdehyde (MDA), glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) according to the instructions of the kit. The test results are filled in Table 2.

[0114] Table 2

[0115]

[0116]

[0117] As shown in Table 2, compared with the blank group, the T-SOD enzyme activity and GSH-PX content of ARPE-19 cells in the model group (blue light irradiation) were significantly reduced, and the MDA content was significantly increased, indicating that the blue light damaged cell model was successful. Compared with the model group, each test group significantly increased the T-SOD enzyme activity and GSH-PX content of ARPE-19 cells, and significantly reduced the MDA content. Among them, the cell antioxidant capacity of the composition treatment group of Examples 1 to 3 was stronger.

[0118] Among the treatment groups of the compositions of Examples 1 to 3, the treatment group of the composition of Example 3 had the highest antioxidant capacity, indicating that the raw materials of the composition of Example 3 produced a better synergistic effect, further improving the antioxidant capacity of the composition.

[0119] Compared with Example 4, radish seed extract is added to the composition of Example 6, and astragalus extract is added to the composition of Example 7, and the antioxidant capacity of the composition is improved. Compared with Examples 6 and 7, astragalus extract and radish seed extract are added to the composition of Example 5 at the same time, and the antioxidant capacity of the composition is greatly improved. It is explained that the composition formed by adding an appropriate amount of astragalus extract and / or radish seed extract to the composition of Example 4 can further enhance the eye protection effect. In particular, the simultaneous addition of astragalus extract and radish seed extract (Example 5) produces an unexpected synergistic effect between the components, which can greatly improve the antioxidant capacity of the composition.

[0120] Compared with Example 5, ursolic acid is added to the composition of Example 2, and the cell antioxidant capacity is further improved.

[0121] Compared with Example 4, the composition of Comparative Example 1 replaced an equal amount of blueberry powder with Cassia seed extract, resulting in a decrease in the antioxidant capacity of cells.

[0122] The above results show that the composition of the present invention can effectively protect human retinal pigment epithelial cells ARPE-19 from blue light damage, relieve visual fatigue and protect vision; and can significantly reduce the oxidative damage of human retinal pigment epithelial cells ARPE-19, and protect vision. The raw materials of the composition of the present invention cooperate with each other and complement each other, so that the eye protection composition can effectively relieve visual fatigue and protect vision for a long time.

[0123] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0124] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A composition for relieving visual fatigue and protecting eyesight, characterized in that: The composition comprises the following raw materials in parts by weight: 1-40 parts of lutein or lutein ester, 1-8 parts of zeaxanthin, 1-500 parts of corn oligopeptides, 1-500 parts of cassia seed extract, 1-500 parts of chrysanthemum extract, 1-250 parts of liquorice extract, and 1-500 parts of anthocyanins.

2. The composition according to claim 1, characterized in that The composition comprises the following raw materials in parts by weight: 5-40 parts of lutein or lutein ester, 2-8 parts of zeaxanthin, 100-500 parts of corn oligopeptides, 100-500 parts of cassia seed extract, 100-500 parts of chrysanthemum extract, 20-250 parts of liquorice extract, and 100-500 parts of anthocyanins; Preferably, the composition comprises the following raw materials in parts by weight: 20-40 parts of lutein or lutein ester, 4-8 parts of zeaxanthin, 200-500 parts of corn oligopeptides, 200-500 parts of cassia seed extract, 200-500 parts of chrysanthemum extract, 50-200 parts of licorice extract, and 200-500 parts of anthocyanins.

3. The composition according to any one of claims 1 to 2, characterized in that The composition comprises at least one of the following raw materials in parts by weight: 1-100 parts of astragalus extract and 1-100 parts of radish seed extract; Preferably, the composition comprises at least one of the following raw materials in parts by weight: 10-100 parts of astragalus extract and 10-100 parts of radish seed extract.

4. The composition according to claim 3, characterized in that The ratio of the total weight of the lutein or lutein ester to zeaxanthin, the weight of corn oligopeptides, the total weight of cassia seed extract, chrysanthemum extract, liquorice extract, astragalus extract and radish seed extract is (32-40): (350-450): (1350-1450), preferably (35-40): (350-400): (1350-1400).

5. The composition according to claim 1 or 3, characterized in that The composition comprises the following raw materials in parts by weight: 1-100 parts of ursolic acid; preferably, the composition comprises the following raw materials in parts by weight: 10-100 parts of ursolic acid.

6. Use of the composition according to any one of claims 1 to 5 in the preparation of products for relieving visual fatigue and protecting eyesight.

7. The use according to claim 6, characterized in that The product is a medicine or a food.

8. A product for relieving visual fatigue and protecting eyesight, characterized in that: The product comprises an active ingredient and a food or pharmaceutically acceptable auxiliary material; the active ingredient comprises the composition according to any one of claims 1 to 5.

9. The product according to claim 8, characterized in that The dosage form of the product is tablet, capsule, granule, powder, solution, powder, pill, emulsion or suspension; and / or, The food or pharmaceutically acceptable excipients include at least one of a sweetener, a sour agent, an anticaking agent and a glidant; Preferably, the sweetener is steviol glycoside; and / or the acidulant is citric acid; and / or the anti-caking agent is magnesium stearate; and / or the glidant is silicon dioxide.

10. The product according to claim 9, characterized in that The product comprises the following components: the composition, 0.01 to 1 part of the sweetener, 0.01 to 1 part of the sour agent, 0.01 to 1 part of the anticaking agent and 0.01 to 1 part of the glidant.

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