Method for preparing liquid fertilizer from lincomycin waste liquid to increase nutritional value of crops

Abstract

The invention belongs to the field of liquid fertilizers, and particularly relates to a method for preparing a liquid fertilizer from lincomycin waste liquid to improve the yield of food crops, which comprises the following steps: step 1, fermentation treatment: obtaining a lincomycin dry product; step 2, fermenting: fermenting for 36-110 hours, and purifying lincomycin from fermentation liquor by using an extraction reagent formed by mixing methanol and ethyl acetate after fermentation is completed; 3, synthesis: adjusting the pH values of the nutrient solution A, the nutrient solution B, the nutrient solution C, the nutrient solution D and the lincomycin to 5.8-6.2 to obtain the liquid fertilizer prepared from the lincomycin waste liquid. The liquid fertilizer prepared from the lincomycin waste liquid can effectively improve the yield of crops.

Description

Technical Field

[0001] The invention belongs to the field of functional liquid fertilizers, in particular to a method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops. Background Art

[0002] Liquid compound fertilizer (Fluid Fertilizer), also known as fluid compound fertilizer, commonly known as liquid fertilizer, is a liquid product containing two or more nutrients required by crops. These nutrients are dissolved in water as solutes to form a solution or suspended in water with the help of a suspending agent to form a suspended paste. Liquid fertilizers have been developed to date and there are many varieties, which can be roughly divided into two categories: liquid nitrogen fertilizer and liquid compound fertilizer. Among them, the effective substances of liquid nitrogen fertilizer include ammonium, nitrate and amide nitrogen, such as liquid ammonia, ammonia water, and nitrogen solution. Liquid compound fertilizer contains all the nutrients required for plant growth, such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and trace elements, and can also be added with organic substances soluble in water (such as humic acid, amino acids, plant growth regulators, etc.). Liquid compound fertilizer can be designed according to the nutritional requirements of crop growth. The fertilizer formula can be adjusted at any time according to the different growth of crops. Scientific formulas can significantly improve fertilizer utilization. The fertilizer efficiency of liquid fertilizers is usually more than 30% higher than that of conventional compound fertilizers.

[0003] Lincomycin, also known as super tylosin, has a chemical name of 3-O-acetyl-4”-O-isovaleryl tylosin, and is a special antibiotic for livestock and poultry. Lincomycin is a new type of macrolide antibiotic obtained by biotransformation of tylosin by Streptomyces thermotolerans, acetylated at the 3-position and isovalerylated at the 4” position. Due to the change in its chemical structure, it has obvious advantages over tylosin in terms of antibacterial spectrum, antibacterial activity, drug resistance, chemical stability, oral absorption and blood drug concentration.

[0004] In the prior art, liquid fertilizer has the following problems: If the nutrient content in liquid fertilizer is insufficient or the ratio is unreasonable, it cannot meet the needs of crop growth, which will lead to low nutritional value of crops. Some liquid fertilizers may contain inappropriate ingredients or have problems in the production process, resulting in poor fertilizer effect. Summary of the invention

[0005] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0006] The technical solution adopted by the present invention to solve the technical problem is: the method of preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops comprises the following steps: Step 1: fermentation treatment: adding 0.1-1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The nutrient solution A comprises: 0.5-1.0 g / L of water-soluble compound fertilizer, 80-100 mg / L of naphthylacetic acid and 30-50 ug / L of brassinolide; The nutrient solution B comprises: 2.0-3.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of naphthaleneacetic acid and 20-30 mg / L of gibberellin; The nutrient solution C comprises: 3.0-4.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of 6-benzylaminoadenine, 140-160 mg / L of chlormequat chloride and 30-40 mg / L of methyl jasmonate; The ingredients of the nutrient solution D are: 5.0-6.0 g / L of water-soluble compound fertilizer, 140-160 mg / L of chlormequat, 80-100 mg / L of methyl jasmonate and 1.0-1.5 g / L of phenylalanine; The pH values ​​of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin are adjusted to 5.8-6.2, thus obtaining liquid fertilizer made from lincomycin waste liquid.

[0007] As a further technical solution of the present invention: the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus; the pore size of the microfiltration membrane is 0.45um.

[0008] As a further technical solution of the present invention: the mass ratio of nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is 100-500: 100-500: 100-500: 100-500: 2-8.

[0009] As a further technical solution of the present invention: the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:5.

[0010] As a further technical solution of the present invention: the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20.

[0011] As a further technical solution of the present invention: the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20.

[0012] As a further technical solution of the present invention: the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20.

[0013] As a further technical solution of the present invention: the preparation process of the water-soluble compound fertilizer is as follows: In the melting pot, add modified ammonium nitrate so that the content of P2O5 is 10wt%-12wt%, and the melted material naturally overflows into the primary mixing pot; In the primary mixing pot, potassium dihydrogen phosphate is added to the molten material flowing in, heated and stirred, and the mixed molten material naturally overflows into the secondary mixing pot; Adding potash fertilizer or potash fertilizer and EDTA complex or potash fertilizer and boron fertilizer into a secondary mixing pot, heating and stirring to obtain a molten mixed slurry, wherein the potash fertilizer is potassium chloride or potassium sulfate; The mixed molten slurry is granulated by a double-shaft differential granulator in a high tower, and is cooled by secondary cooling in a fluidized bed to obtain a fully water-soluble nitro compound fertilizer material; or modified ammonium nitrate: ammonium sulfate: industrial monoammonium phosphate: potassium sulfate: EDTA complex or boron fertilizer = 1: 0.10-0.95: 0.05-0.50: 0.25-0.75: 0-0.2.

[0014] The beneficial effects of the present invention are as follows: The fermentation treatment of the present invention comprises the following steps: adding 0.1-1% w / v of an adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by using a microfiltration water membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; and fermenting: firstly performing fed-batch fermentation, and then converting to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, Isobutanol is added to the fermentation liquid, and after fermentation continues for a period of time, glucose is added to the fermentation liquid; after fermentation continues for a period of time, the fermentation liquid enters a repeated feeding batch fermentation stage, a part of the fermentation liquid is discharged, fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Synthesis: The nutrient solution A comprises: 0.5-1.0 g / L of water-soluble compound fertilizer, 80-100 mg / L of naphthaleneacetic acid and 30-50 ug / L of brassinolide; The nutrient solution B comprises: 2.0-3.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of naphthaleneacetic acid and 20-30 mg / L of gibberellin; The nutrient solution C comprises: 3.0-4.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of 6-benzylaminoadenine, 140-160 mg / L of chlormequat chloride and 30-40 mg / L of methyl jasmonate; The ingredients of the nutrient solution D are: 5.0-6.0 g / L of water-soluble compound fertilizer, 140-160 mg / L of chlormequat, 80-100 mg / L of methyl jasmonate and 1.0-1.5 g / L of phenylalanine; The pH of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is adjusted to 5.8-6.2, thus obtaining a liquid fertilizer made from lincomycin waste liquid; Among them, the role of brassinolide in fertilizer is mainly reflected in the following aspects: 1. Promote plant cell elongation and division Brassinolide is a natural plant hormone that promotes the elongation and division of plant cells, thereby increasing the height and leaf area of ​​the plant. This is of great significance for improving the photosynthesis efficiency and biomass accumulation of crops. By increasing the leaf area, plants can capture more light energy and convert it into chemical energy, thereby promoting the growth and development of crops.

[0015] 2. Enhance plant resistance Brassinolide can significantly improve the stress resistance of plants, including drought resistance and salt tolerance. In harsh environments such as drought or saline-alkali land, plant growth is often restricted and yields are reduced. Brassinolide can help plants better adapt to these adverse environments by regulating physiological metabolic processes in plants. For example, it can promote the development of plant roots and improve the root system's ability to absorb water and nutrients, thereby enhancing the plant's drought resistance. At the same time, brassinolide can also regulate the osmotic pressure and ion balance in plants, improving the plant's tolerance to salt-alkali stress.

[0016] 3. Promote crop growth and development Brassinolide plays an important role in the growth and development of crops. It can promote the nutritional growth of crops, such as promoting root development and increasing leaf area. At the same time, brassinolide can also promote the reproductive growth of crops, such as increasing pollen germination rate and promoting pollen tube growth, thereby increasing the chance of successful fertilization and improving crop fruiting rate and yield.

[0017] 4. Improve crop yield and quality Because brassinolide has the above-mentioned multiple physiological functions, it can significantly improve the yield and quality of crops. In practical applications, farmers can promote the growth and development of crops by spraying brassinolide solution, thereby achieving the effect of increasing production. At the same time, brassinolide can also improve the quality of crops, such as increasing the sweetness, color and aroma of fruits, making agricultural products more competitive in the market.

[0018] Among them, the liquid fertilizer prepared from the lincomycin waste liquid of the present invention can effectively increase the nutritional value of crops. DETAILED DESCRIPTION

[0019] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0020] Embodiment 1 A method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 0.1% w / v of an adsorbent to the lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The nutrient solution A comprises: 0.5 g / L of water-soluble compound fertilizer, 80 mg / L of naphthylacetic acid and 30 ug / L of brassinolide; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:5; The composition of the nutrient solution B is: 2.0 g / L of water-soluble compound fertilizer, 200 mg / L of naphthaleneacetic acid and 20 mg / L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The nutrient solution C comprises: 3.0 g / L water-soluble compound fertilizer, 20 mg / L 6-benzylaminopurine, 140 mg / L chlormequat chloride and 30 mg / L methyl jasmonate; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The ingredients of the nutrient solution D are: 5.0 g / L of water-soluble compound fertilizer, 140 mg / L of chlormequat chloride, 80 mg / L of methyl jasmonate and 1.0 g / L of phenylalanine; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The pH of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is adjusted to 5.8-6.2; Wherein, the mass ratio of nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is 100:100:100:100:2; Among them, the preparation process of water-soluble compound fertilizer is as follows: In the melting pot, add modified ammonium nitrate so that the content of P2O5 is 10wt%, and the melted material naturally overflows into the primary mixing pot; In the primary mixing pot, potassium dihydrogen phosphate is added to the molten material flowing in, heated and stirred, and the mixed molten material naturally overflows into the secondary mixing pot; Adding potash fertilizer or potash fertilizer and EDTA complex or potash fertilizer and boron fertilizer into a secondary mixing pot, heating and stirring to obtain a molten mixed slurry, wherein the potash fertilizer is potassium chloride or potassium sulfate; The mixed molten slurry is granulated by a double-shaft differential granulator in a high tower, and is cooled by secondary cooling in a fluidized bed to obtain a fully water-soluble nitro compound fertilizer material; or modified ammonium nitrate: ammonium sulfate: industrial monoammonium phosphate: potassium sulfate: EDTA complex or boron fertilizer = 1:0.10:0.05:0.25:0.1.

[0021] Embodiment 2 A method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 0.5% w / v of an adsorbent to the lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 7%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The nutrient solution A comprises: 0.8 g / L of water-soluble compound fertilizer, 90 mg / L of naphthylacetic acid and 40 ug / L of brassinolide; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:5; The composition of the nutrient solution B is: 2.5 g / L water-soluble compound fertilizer, 230 mg / L naphthylacetic acid and 25 mg / L gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The nutrient solution C comprises: 3.5 g / L water-soluble compound fertilizer, 220 mg / L 6-benzylaminopurine, 150 mg / L chlormequat chloride and 35 mg / L methyl jasmonate; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The ingredients of the nutrient solution D are: 5.5 g / L of water-soluble compound fertilizer, 150 mg / L of chlormequat chloride, 90 mg / L of methyl jasmonate and 1.2 g / L of phenylalanine; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The pH of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is adjusted to 5.8-6.2; Wherein, the mass ratio of nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is 300:300:300:300:5; Among them, the preparation process of water-soluble compound fertilizer is as follows: In the melting pot, add modified ammonium nitrate to make the content of P2O5 11wt%, and the melted material naturally overflows into the primary mixing pot; In the primary mixing pot, potassium dihydrogen phosphate is added to the molten material flowing in, heated and stirred, and the mixed molten material naturally overflows into the secondary mixing pot; Adding potash fertilizer or potash fertilizer and EDTA complex or potash fertilizer and boron fertilizer into a secondary mixing pot, heating and stirring to obtain a molten mixed slurry, wherein the potash fertilizer is potassium chloride or potassium sulfate; The mixed molten slurry is granulated by a double-shaft differential granulator in a high tower, and is cooled by secondary cooling in a fluidized bed to obtain a fully water-soluble nitro compound fertilizer material; or modified ammonium nitrate: ammonium sulfate: industrial monoammonium phosphate: potassium sulfate: EDTA complex or boron fertilizer = 1:0.55:025:0.55:0.1.

[0022] Embodiment 3 A method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to an embodiment of the present invention comprises the following steps: Step 1: fermentation treatment: adding 1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 10%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The nutrient solution A comprises: 1.0 g / L water-soluble compound fertilizer, 100 mg / L naphthylacetic acid and 50 ug / L brassinolide; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:5; The nutrient solution B comprises: 3.0 g / L water-soluble compound fertilizer, 250 mg / L naphthylacetic acid and 30 mg / L gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The nutrient solution C comprises: 4.0 g / L water-soluble compound fertilizer, 250 mg / L 6-benzylaminopurine, 160 mg / L chlormequat chloride and 40 mg / L methyl jasmonate; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The ingredients of the nutrient solution D are: 6.0 g / L of water-soluble compound fertilizer, 160 mg / L of chlormequat chloride, 100 mg / L of methyl jasmonate and 1.5 g / L of phenylalanine; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The pH of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is adjusted to 5.8-6.2; Wherein, the mass ratio of nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is 500:500:500:500:8; Among them, the preparation process of water-soluble compound fertilizer is as follows: In the melting pot, add modified ammonium nitrate to make the content of P2O5 12wt%, and the melted material naturally overflows into the primary mixing pot; In the primary mixing pot, potassium dihydrogen phosphate is added to the molten material flowing in, heated and stirred, and the mixed molten material naturally overflows into the secondary mixing pot; Adding potash fertilizer or potash fertilizer and EDTA complex or potash fertilizer and boron fertilizer into a secondary mixing pot, heating and stirring to obtain a molten mixed slurry, wherein the potash fertilizer is potassium chloride or potassium sulfate; The mixed molten slurry is granulated by a double-shaft differential granulator in a high tower, and is cooled by secondary cooling in a fluidized bed to obtain a fully water-soluble nitro compound fertilizer material; or modified ammonium nitrate: ammonium sulfate: industrial monoammonium phosphate: potassium sulfate: EDTA complex or boron fertilizer = 1: 0.95: 0.50: 0.75: 0.2.

[0023] Comparative Example 1 Comparative Example 1 adopts the preparation process of a liquid fertilizer, comprising the following steps: The nutrient solution A comprises: 0.5 g / L water-soluble compound fertilizer, 80 mg / L naphthylacetic acid and 30 ug / L brassinolide; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:5; The composition of the nutrient solution B is: 2.0 g / L of water-soluble compound fertilizer, 200 mg / L of naphthaleneacetic acid and 20 mg / L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The nutrient solution C comprises: 3.0 g / L water-soluble compound fertilizer, 200 mg / L 6-benzylaminopurine, 140 mg / L chlormequat chloride and 30 mg / L methyl jasmonate; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The ingredients of the nutrient solution D are: 5.0 g / L of water-soluble compound fertilizer, 140 mg / L of chlormequat chloride, 80 mg / L of methyl jasmonate and 1.0 g / L of phenylalanine; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:20; The pH values ​​of the nutrient solution A, nutrient solution B, nutrient solution C and nutrient solution D are adjusted to 5.8-6.2 to obtain liquid fertilizer.

[0024] Performance Testing: For the lincomycin finished liquid composite liquid fertilizers prepared in Examples 1-3 and Comparative Example 1, field tests were conducted on rice cultivation. Based on the plant height and root length of rice, the effects of different lincomycin finished liquid composite fertilizers on rice production were obtained, and Table 1 was obtained:

[0025] It can be seen from the above table that the liquid fertilizer prepared from the lincomycin waste liquid of the present invention can effectively increase the nutritional value of crops.

[0026] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops, characterized in that: The following steps are involved: Step 1: fermentation treatment: adding 0.1-1% w / v of adsorbent to lincomycin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a lincomycin dry product; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation liquid, and after fermentation for a period of time, glucose is added to the fermentation liquid; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, lincomycin is purified from the fermentation liquid using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The nutrient solution A comprises: 0.5-1.0 g / L of water-soluble compound fertilizer, 80-100 mg / L of naphthylacetic acid and 30-50 ug / L of brassinolide; The nutrient solution B comprises: 2.0-3.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of naphthaleneacetic acid and 20-30 mg / L of gibberellin; The nutrient solution C comprises: 3.0-4.0 g / L of water-soluble compound fertilizer, 200-250 mg / L of 6-benzylaminoadenine, 140-160 mg / L of chlormequat chloride and 30-40 mg / L of methyl jasmonate; The ingredients of the nutrient solution D are: 5.0-6.0 g / L of water-soluble compound fertilizer, 140-160 mg / L of chlormequat, 80-100 mg / L of methyl jasmonate and 1.0-1.5 g / L of phenylalanine; The pH values ​​of the nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin are adjusted to 5.8-6.2, thus obtaining liquid fertilizer made from lincomycin waste liquid.

2. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 1, characterized in that: The acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus.

3. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 2, characterized in that: The pore size of the microfiltration membrane is 0.45 um.

4. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 3, characterized in that: The mass ratio of nutrient solution A, nutrient solution B, nutrient solution C, nutrient solution D and lincomycin is 100-500: 100-500: 100-500: 100-500: 2-8.

5. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 4, characterized in that: The mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:5:

5.

6. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 5, characterized in that: The mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:

20.

7. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 6, characterized in that: The mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 15:20:

20.

8. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 8, characterized in that: The mass ratio of nitrogen, phosphorus and potassium in water-soluble compound fertilizer is 15:20:

20.

9. The method for preparing liquid fertilizer from lincomycin waste liquid to increase the nutritional value of crops according to claim 9, characterized in that: The preparation process of water-soluble compound fertilizer is as follows: In the melting pot, add modified ammonium nitrate so that the content of P2O5 is 10wt%-12wt%, and the melted material naturally overflows into the primary mixing pot; In the first-stage mixing pot, potassium dihydrogen phosphate is added to the molten material flowing in, heated and stirred, and the mixed molten material naturally overflows into the second-stage mixing pot; Adding potash fertilizer or potash fertilizer and EDTA complex or potash fertilizer and boron fertilizer into a secondary mixing pot, heating and stirring to obtain a molten mixed slurry, wherein the potash fertilizer is potassium chloride or potassium sulfate; The mixed molten slurry is granulated by a double-shaft differential granulator in a high tower, and is cooled by secondary cooling in a fluidized bed to obtain a fully water-soluble nitro compound fertilizer material; or modified ammonium nitrate: ammonium sulfate: industrial monoammonium phosphate: potassium sulfate: EDTA complex or boron fertilizer = 1: 0.10-0.95: 0.05-0.50: 0.25-0.75: 0-0.2.

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