The invention discloses a strain capable of degrading 4apos; shinella QZW1 of-(hexyloxy)-4-biphenyl formonitrile and application of Shinella QZW1

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a Shinella sp. QZW1 for degrading 4 '-(hexyloxy)-4-biphenyl formonitrile and application of the Shinella sp. QZW1. The invention provides a Shinella sp. QZW1, the preservation number is GDMCC NO: 64864, and the Shinella sp. QZW1 has the capability of degrading cyano liquid crystal monomers, especially 4-HBC. The result of the embodiment shows that under the condition that glucose is not added, the degradation efficiency of the Shinella sp. QZW1 provided by the invention on 4-HBC with the concentration of 0.25 mg / L, 0.5 mg / L and 1.0 mg / L is 85.78%, 64.27% and 44.58% respectively; and under the condition that glucose is added, the degradation efficiency of the Shinella sp. QZW1 on 4-HBC is further improved. The Shinella sp. QZW1 provided by the invention has a good application prospect in biodegradation and bioremediation of liquid crystal monomer pollutants.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and specifically relates to a strain of Bacillus shenkelii QZW1 capable of degrading 4'-(hexyloxy)-4-biphenylcarbonitrile and application thereof. Background Art

[0002] Liquid crystal monomers are a class of synthetic organic chemicals that are used as special functional materials in liquid crystal displays. With the rapid replacement of electronic devices, a large number of discarded liquid crystal displays (LCD screens) have become part of electronic waste. Useful liquid crystal monomers are applied to liquid crystal displays by physical addition rather than chemical bonding, which makes them easily released into the surrounding environment during the production, use, treatment and disposal of liquid crystal display-related equipment. Nowadays, the presence of liquid crystal monomers has been widely detected in environmental samples including the atmosphere, indoor dust, soil, river and lake water bodies and sediments, as well as organisms such as vegetables, fruits and poultry, and human tissue samples. 4′-(Hexyloxy)-4-biphenylcarbonitrile (4-HBC) is a typical liquid crystal monomer containing a cyano functional group, which has been widely detected in environmental samples such as electronic waste dismantling areas and estuary deltas. In addition, a large number of studies have shown that liquid crystal monomer pollutants including 4-HBC have environmental persistence, bioaccumulation and biotoxicity, so liquid crystal monomers have become a new pollutant that has attracted much attention in recent years. Therefore, there is an urgent need to develop effective treatment and remediation technologies.

[0003] At present, the degradation and conversion technologies for liquid crystal monomer pollutants are very limited, and only a few studies have achieved the degradation and removal of liquid crystal monomers through photoconversion and Fenton oxidation technology. Microbial degradation, as a means of bioremediation, can convert complex organic pollutants into harmless or low-toxic small molecules, and has the advantages of being green, efficient and low-cost. However, existing bioremediation technologies still face challenges in degrading liquid crystal monomer pollutants, mainly due to the lack of efficient and stable liquid crystal monomer degradation functional bacteria. Therefore, it is of great significance to screen functional bacteria that can efficiently degrade liquid crystal monomers and develop liquid crystal monomer pollution remediation technologies based on microbial enhancement. Summary of the invention

[0004] The present invention aims to provide a strain of Bacillus shenkelii QZW1 capable of degrading 4′-(hexyloxy)-4-biphenylcarbonitrile and its application, degrading and repairing cyano liquid crystal monomer pollutants, especially achieving efficient degradation of 4′-(hexyloxy)-4-biphenylcarbonitrile.

[0005] The invention provides a strain of Bacillus shenkelii QZW1, with the deposit number being GDMCCNO:64864.

[0006] The present invention also provides a bacterial agent, which includes the Bacillus shenkelii QZW1 described in the above technical solution.

[0007] The present invention also provides the use of Bacillus shenkelii QZW1 described in the above technical solution or the bacterial agent described in the above technical solution in degrading cyano liquid crystal monomer pollutants; the cyano liquid crystal monomer pollutants include 4'-(hexyloxy)-4-biphenylcarbonitrile.

[0008] Preferably, the concentration of the cyano liquid crystal monomer in the cyano liquid crystal monomer contaminant is 0.25-3 mg / L.

[0009] The present invention also provides a method for degrading cyano liquid crystal monomer pollutants, comprising the following steps: inoculating the cyano liquid crystal monomer pollutants to be degraded with the Bacillus shenkelii QZW1 or the bacterial agent described in the above technical solution, and culturing at 30° C.;

[0010] The cyano liquid crystal monomer contaminant includes 4'-(hexyloxy)-4-biphenylcarbonitrile.

[0011] Preferably, the initial bacterial dose OD of the Bacillus shenkelii QZW1 is 600 It is 0.05~0.4.

[0012] Preferably, the concentration of the cyano liquid crystal monomer in the cyano liquid crystal monomer pollutant to be degraded is 0.25-3 mg / L.

[0013] Preferably, the method further comprises: adding glucose to the liquid crystal monomer pollutant to be degraded;

[0014] The added amount of glucose is ≤1000 mg / L.

[0015] Preferably, the added amount of glucose is 50-1000 mg / L.

[0016] Preferably, the added amount of glucose is 100-500 mg / L.

[0017] Beneficial effects:

[0018] The present invention provides a strain of Bacillus shenkelii QZW1, with a deposit number of GDMCC NO: 64864, which has the ability to degrade cyano liquid crystal monomers, especially 4-HBC. The results of the embodiments show that under the condition of not adding glucose, the degradation efficiency of Bacillus shenkelii QZW1 provided by the present invention for 0.25 mg / L, 0.5 mg / L and 1.0 mg / L 4-HBC is 85.78%, 64.27% and 44.58% respectively; and under the condition of adding glucose, the degradation efficiency of Bacillus shenkelii QZW1 for 4-HBC is further improved. The Bacillus shenkelii QZW1 provided by the present invention has good application prospects in the biodegradation and bioremediation of liquid crystal monomer pollutants.

[0019] Biological deposit information

[0020] Shinella sp. QZW1, biologically classified as Shinella sp., was deposited in the Guangdong Provincial Microbiological Culture Collection Center on July 12, 2024, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with the deposit number GDMCC NO: 64864. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0022] Figure 1 is the phylogenetic tree of Bacillus shenkelii QZW1;

[0023] Figure 2 This is the degradation kinetics diagram of 4-HBC by Bacillus shenkelii QZW1;

[0024] Figure 3 This is the effect of glucose concentration on the degradation performance of 4-HBC by Bacillus shenkelii QZW1;

[0025] Figure 4 This is the graph showing the effect of bacterial dosage on 4-HBC degradation performance of Bacillus shenkelii QZW1;

[0026] Figure 5 This is the degradation performance diagram of Bacillus shenkelii QZW1 on 4-HBC with different initial concentrations. DETAILED DESCRIPTION

[0027] The invention provides a strain of Bacillus shenkelii QZW1, with the deposit number being GDMCCNO:64864.

[0028] The present invention also provides a bacterial agent, which includes the Bacillus shenkelii QZW1 described in the above technical solution.

[0029] The present invention obtains a strain of Bacillus shenkelii QZW1 by domesticating and screening from a soil sample of an electronic waste dismantling site in Qingyuan City, Guangdong Province, which has the ability to degrade cyano liquid crystal monomers, especially 4-HBC. Using the Bacillus shenkelii QZW1 of the present invention or a bacterial agent with the Bacillus shenkelii QZW1 as an active ingredient, liquid crystal monomer pollutants can be efficiently degraded, and has good application prospects in the biodegradation and bioremediation of liquid crystal monomer pollutants.

[0030] In view of the effects of Bacillus shenkelii QZW1 and the bacterial agent provided by the present invention, the use of Bacillus shenkelii QZW1 or the bacterial agent described in the above technical solution in degrading cyano liquid crystal monomer pollutants also belongs to the protection scope of the present invention. In the present invention, as a specific embodiment, the cyano liquid crystal monomer pollutant includes 4′-(hexyloxy)-4-biphenylcarbonitrile. As a specific embodiment, the concentration of cyano liquid crystal monomer in the cyano liquid crystal monomer pollutant is 0.25 to 3 mg / L; as another specific embodiment, the concentration of cyano liquid crystal monomer in the cyano liquid crystal monomer pollutant is 0.5 to 2.0 mg / L; as another specific embodiment, the concentration of cyano liquid crystal monomer in the cyano liquid crystal monomer pollutant is 1.0 to 1.5 g / L.

[0031] The present invention also provides a method for degrading cyano liquid crystal monomer pollutants, comprising the following steps: inoculating the Bacillus shenkelii QZW1 or the bacterial agent described in the above technical scheme into the cyano liquid crystal monomer pollutants to be degraded, and culturing at 30°C; the cyano liquid crystal monomer pollutants include 4′-(hexyloxy)-4-biphenylcarbonitrile.

[0032] In the present invention, the cyano liquid crystal monomer pollutant to be degraded is inoculated with the Bacillus shenkelii QZW1 or the bacterial agent described in the above technical solution and cultured at 30°C. In the present invention, as a specific embodiment, the initial bacterial dose OD of the Bacillus shenkelii QZW1 is 600 is 0.05 to 0.4; as another specific embodiment, the initial bacterial dose OD of the Bacillus shenkelii QZW1 is 600 is 0.1 to 0.3; as another specific embodiment, the initial bacterial dose OD of the Bacillus shenkelii QZW1 is 600 The initial bacterial dose of Bacillus shenkelii QZW1 affects the degradation efficiency of Bacillus shenkelii QZW1 on 4-HBC. 600 As the value increases, the degradation efficiency of 4-HBC gradually increases, but it cannot be increased infinitely. When the initial bacterial dosage OD value specified in the present invention is exceeded, 600 When it is 0.4, the degradation efficiency of 4-HBC gradually decreases.

[0033] In the present invention, as a specific embodiment, the cyano liquid crystal monomer pollutant to be degraded includes a cyano liquid crystal monomer concentration of 0.25 to 3 mg / L; as another specific embodiment, the cyano liquid crystal monomer concentration in the cyano liquid crystal monomer pollutant is 0.5 to 2.0 mg / L; as another specific embodiment, the cyano liquid crystal monomer concentration in the cyano liquid crystal monomer pollutant is 1.0 to 1.5 g / L.

[0034] In the present invention, as a specific embodiment, the culture time is 1 to 7 days; as another specific embodiment, the culture time is 2 to 6 days; as another specific embodiment, the culture time is 3 to 5 days; as another specific embodiment, the culture time is 4 days.

[0035] In the present invention, the Bacillus shenkelii QZW1 or the bacterial agent described in the above technical solution is inoculated into the cyano liquid crystal monomer pollutant to be degraded, and cultured at 30° C. The degradation efficiency of 0.25 mg / L, 0.5 mg / L, 1.0 mg / L, 2.0 mg / L and 3.0 mg / L 4-HBC is 85.78%, 64.27%, 44.58%, 34.75% and 28.76% respectively. It can be seen that the Bacillus shenkelii QZW1 or the bacterial agent provided by the present invention can achieve the degradation of 4-HBC.

[0036] In the present invention, as a specific embodiment, glucose is added to the liquid crystal monomer pollutant to be degraded. As a specific embodiment, the amount of glucose added is ≤1000 mg / L; as another specific embodiment, the amount of glucose added is 50-1000 mg / L; as another specific embodiment, the amount of glucose added is 100-500 mg / L.

[0037] In the present invention, after inoculating Bacillus shenkelii QZW1 or bacterial agent, glucose is further added. The results of the embodiment show that when the glucose addition amount is 50, 100 mg / L, 200 mg / L, 500 mg / L and 1000 mg / L, the degradation efficiency of Bacillus shenkelii QZW1 on 1.0 mg / L 4-HBC is 55.23%, 74.04%, 79.82%, 84.31% and 74.01% respectively. It can be seen that appropriately increasing the concentration of glucose can significantly improve the biodegradation of 4-HBC by Bacillus shenkelii QZW1.

[0038] To further illustrate the present invention, a strain of Bacillus shenkelii QZW1 for degrading 4′-(hexyloxy)-4-biphenylcarbonitrile and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0039] The culture medium components used in the embodiment of the present invention are as follows:

[0040] MSM culture medium: 1.5 g / L KH2PO4, 3.0 g / L K2HPO4, 0.5 g / L NaCl, 1.0 g / L (NH4)2SO4, 2.0 mL trace element stock solution and the balance of water, pH 7.0-7.2; wherein the trace element stock solution comprises: 4 g / LMgSO4, 4 g / LZnSO4, 1 g / L CuSO4, 1 g / LMnSO4, 1 g / LFeSO4·7H2O and 1 g / L CaCl2;

[0041] BEM solid medium: 3.0 g / L beef extract, 10.0 g / L peptone, 5.0 g / L NaCl, 20.0 g / L agar and the balance water, pH 7.0-7.2;

[0042] BEM liquid culture medium: 3.0 g / L beef extract, 10.0 g / L peptone, 5.0 g / L NaCl and the balance water, pH 7.0-7.2.

[0043] Example 1

[0044] Screening and identification of strains

[0045] 1. Screening and isolation of strains

[0046] (1) Weigh 5 g of liquid crystal monomer contaminated soil and add it to 200 mL of inorganic salt medium (MSM) containing 1.0 mg / L 4-HBC, and culture it in a shaker at 30°C and 160 rpm for one week; transfer 20 mL of the culture solution to 200 mL of MSM medium containing 2.0 mg / L 4-HBC, and culture it under the same conditions for one week; repeat the above transfer and culture for 6 times, and the concentration of 4-HBC during the culture process is set to 1.0, 2.0, 4.0, 6.0, 8.0 and 10.0 mg / L.

[0047] (2) Take an appropriate amount of acclimatization culture medium and dilute it with phosphate buffered saline (PBS) for 10 4 ~10 6 times, and evenly spread them on beef extract peptone (BEM) solid plates, and culture them in a 30℃ constant temperature incubator until colonies of different shapes grow on the plates. Use an inoculation loop to pick up an appropriate amount of colonies and streak them on the BEM solid plates, and purify each colony until a single colony is obtained.

[0048] (3) Use an inoculation loop to pick an appropriate amount of single colonies and inoculate them into BEM liquid culture medium, place them in a shaker at 30°C and 160rpm for enrichment culture, collect bacterial cells by centrifugation at 4°C and 6500rpm, wash the bacteria three times with PBS, and resuspend them in PBS to obtain bacterial suspensions of appropriate concentrations for each strain. Take an appropriate amount of bacterial suspension and add it to 20mL of MSM culture medium containing 1.0mg / L4-HBC, and place it in a shaker at 30°C and 160rpm for 7 days. Use an equal proportion of analytical pure dichloromethane and n-hexane mixed solvent to extract the above culture solution three times, collect the organic phases of the three extractions and mix them in a chicken heart bottle, evaporate them at 40°C to near dryness, and make up to volume with 20mL of chromatographic grade acetonitrile. Use high performance liquid chromatography to analyze the concentration of residual 4-HBC and calculate the 4-HBC degradation efficiency.

[0049] 2. Identification of strains

[0050] After the above screening, separation and testing, a strain capable of rapidly degrading 4-HBC was obtained. The colonies of the strain grown on BEM solid medium were round, milky white, smooth and with neat edges. The homology comparison of the 16S rRNA gene sequence with the NCBI database revealed that the strain had a high degree of similarity with Shinella spp., and its phylogenetic tree is shown in Figure 1 Therefore, the strain was identified as Shinella sp. and named Shinella sp. QZW1. It was deposited in Guangdong Microbiological Culture Collection Center on July 12, 2024, with the address: 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou, and the deposit number is GDMCCNO: 64864.

[0051] Example 2

[0052] Degradation Kinetics of 4-HBC by Bacillus shenkelii QZW1

[0053] (1) Take an appropriate amount of the preservation solution of Bacillus shenkelii QZW1, inoculate it into BEM liquid culture medium, place it in a shaker at 30°C and 160 rpm for enrichment culture, collect bacterial cells by centrifugation at 4°C and 6500 rpm, wash the bacteria three times with PBS, and resuspend them in PBS to obtain a bacterial suspension of a certain concentration.

[0054] (2) Experimental Group 1 (QZW1): Take an appropriate amount of bacterial suspension and inoculate it into 20 mL of MSM medium containing 1.0 mg / L 4-HBC, so that the OD of the experimental system (initial bacterial dose) is 600 The value is 0.2;

[0055] Experimental group 2 (QZW1 + glucose): Take an appropriate amount of bacterial suspension and inoculate it into 20 mL of MSM medium containing 1.0 mg / L 4-HBC and 500 mg / L glucose, so that the (initial bacterial dose) OD of the experimental system is 600 The value is 0.2;

[0056] Control group: No bacterial suspension was added, and MSM medium containing 1.0 mg / L 4-HBC was used as the control.

[0057] (3) Set sampling points on the 0th, 1st, 2nd, 3rd, 5th and 7th days. In step (2), set 3 parallel samples for each treatment and each time point in experimental groups 1 to 2 and the control group. Seal with a breathable sealing film and culture in a shaker at 30°C and 160rpm for 7 days. Use an equal amount of analytical grade dichloromethane and n-hexane mixed solvent to extract the above degradation liquid three times, collect the three extracted organic phases and mix them in a chicken heart bottle, evaporate them at 40°C until almost dry, make up to volume with 20mL of chromatographic grade acetonitrile, analyze the residual concentration of 4-HBC by high performance liquid chromatography, and calculate the degradation efficiency of 4-HBC at different initial concentrations. The results are as follows Figure 2 And as shown in Table 1.

[0058] Table 1 Degradation efficiency of 4-HBC in different treatment groups

[0059] time 0d 1d 2d 3d 5d 7d Experimental Group 1 0.00％ 16.07％ 30.60％ 34.98％ 43.87％ 44.71％ Experimental Group 2 0.00％ 22.83％ 46.88％ 54.99％ 81.66％ 83.69％ Control group 0.00％ 1.38％ 1.36％ 2.34％ 3.28％ 3.46％

[0060] According to Table 1 and Figure 2 It can be seen that without adding glucose, the degradation efficiency of 1.0 mg / L 4-HBC was 44.71% after 7 days of culture; when 500 mg / L glucose was added as a cometabolism substrate, the degradation efficiency of 4-HBC increased significantly to 83.69% after 7 days of culture. It can be seen that Bacillus shenkelii QZW1 has a high degradation ability for 4-HBC, and adding glucose as a cometabolism substrate can significantly improve the degradation efficiency of 4-HBC.

[0061] Example 3

[0062] Effect of Glucose Concentration on the Performance of Bacillus shenkelii QZW1 on 4-HBC

[0063] (1) According to the method and steps of Example 2, enrichment culture of Bacillus shenkelii QZW1 was carried out, and a certain concentration of bacterial suspension was prepared by centrifugation and washing. An appropriate amount of bacterial suspension was inoculated into 20 mL of MSM medium containing 1.0 mg / L 4-HBC and different concentrations of glucose, wherein the glucose concentration was set to 0, 50, 100, 200, 500 and 1000 mg / L, respectively, and the initial bacterial dosage OD 600 is 0.2.

[0064] (2) Three parallel samples were set up for each treatment. After sealing with a breathable sealing film, they were placed in a shaker at 30°C and 160 rpm for 7 days. The above degradation solution was extracted three times with an equal amount of analytical grade dichloromethane and n-hexane mixed solvent. The three extracted organic phases were collected and mixed in a chicken heart bottle, and then rotary evaporated at 40°C to near dryness. The volume was fixed with 20 mL of chromatographic grade acetonitrile, and the residual concentration of 4-HBC was analyzed by high performance liquid chromatography, and the degradation efficiency of 4-HBC at different initial concentrations was calculated. The results are shown in Figure 3 And as shown in Table 2.

[0065] Table 2 Degradation efficiency of 4-HBC at different glucose concentrations

[0066] Glucose concentration (mg / L) 0 50 100 200 500 1000 Degradation rate (%) 44.28 55.23 74.04 79.82 84.31 74.01

[0067] According to Table 2 and Figure 3 It can be seen that when the initial glucose concentration is less than or equal to 500 mg / L, appropriately increasing the glucose concentration can significantly improve the biodegradation of 4-HBC by Bacillus shenkelii QZW1; however, when too high a concentration of glucose (greater than 500 mg / L) is added, the degradation efficiency of 4-HBC begins to decrease. Therefore, when using Bacillus shenkelii QZW1 to degrade 4-HBC in water environments, the added glucose concentration needs to be reasonably controlled.

[0068] Example 4

[0069] Effect of the dosage of Bacillus shenkelii QZW1 on the degradation performance of 4-HBC

[0070] (1) According to the method and steps of Example 2, enrichment culture of Bacillus shenkelii QZW1 was carried out, and a bacterial suspension of a certain concentration was prepared by centrifugation and washing. An appropriate amount of the bacterial suspension was inoculated into 20 mL of MSM medium containing 1.0 mg / L 4-HBC and 500 mg / L glucose. The initial bacterial dosage OD 600 Set to 0.05, 0.1, 0.15, 0.2, 0.3 and 0.4 respectively.

[0071] (2) Three parallel samples were set up for each treatment. After sealing with a breathable sealing film, they were placed in a shaker at 30°C and 160 rpm for 7 days. The above degradation solution was extracted three times with an equal amount of analytical grade dichloromethane and n-hexane mixed solvent. The three extracted organic phases were collected and mixed in a chicken heart bottle, and then rotary evaporated at 40°C to near dryness. The volume was fixed with 20 mL of chromatographic grade acetonitrile, and the residual concentration of 4-HBC was analyzed by high performance liquid chromatography, and the degradation efficiency of 4-HBC at different initial concentrations was calculated. The results are shown in Figure 4 And as shown in Table 3.

[0072] Table 3 Degradation efficiency of 4-HBC at different initial bacterial dosages

[0073] <![CDATA[Initial inoculum OD 600 > 0.05 0.1 0.15 0.2 0.3 0.4 Degradation rate (%) 21.97 61.51 82.91 84.21 84.06 81.66

[0074] According to Table 3 and Figure 4 It can be seen that when the initial bacterial dosage OD 600 When the value was less than 0.2, the bacterial dosage significantly affected the degradation efficiency of 4-HBC by Bacillus shenkelii QZW1. 600 When the value is greater than or equal to 0.2, the bacterial dosage has no significant effect on the degradation efficiency of 4-HBC, so the bacterial dosage OD 600 The value was 0.2 for subsequent experiments.

[0075] Example 5

[0076] Degradation performance of 4-HBC with different initial concentrations by Bacillus shenkelii QZW1

[0077] (1) According to the method and steps of Example 2, enrichment culture of Bacillus shenkelii QZW1 was carried out, and a certain concentration of bacterial suspension was prepared by centrifugation and washing. Experimental Group 1: An appropriate amount of bacterial suspension was inoculated into 20 mL of MSM medium containing different concentrations of 4-HBC, wherein the concentrations of 4-HBC were set to 0.25, 0.5, 1.0, 2.0 and 3.0 mg / L, respectively, and the initial bacterial dosage OD 600 is 0.2;

[0078] Experimental group 2: Take an appropriate amount of bacterial suspension and inoculate it into 20 mL of MSM medium containing 500 mg / L glucose and different concentrations of 4-HBC, where the concentrations of 4-HBC were set to 0.25, 0.5, 1.0, 2.0 and 3.0 mg / L, respectively. The initial bacterial dose OD 600 is 0.2.

[0079] (2) Three parallel samples were set up for each treatment. After sealing with a breathable sealing film, they were placed in a shaker at 30°C and 160 rpm for 7 days. The above degradation solution was extracted three times with an equal amount of analytical grade dichloromethane and n-hexane mixed solvent. The three extracted organic phases were collected and mixed in a chicken heart bottle, and then rotary evaporated at 40°C to near dryness. The volume was fixed with 20 mL of chromatographic grade acetonitrile, and the residual concentration of 4-HBC was analyzed by high performance liquid chromatography, and the degradation efficiency of 4-HBC at different initial concentrations was calculated. The results are shown in Figure 5 And as shown in Table 4.

[0080] Table 4 Degradation efficiency of 4-HBC at different initial concentrations

[0081]

[0082] According to Table 4 and Figure 5 It can be seen that the initial concentration of 4-HBC significantly affects the degradation efficiency of Bacillus shenkelii QZW1; the addition of glucose has a significant promoting effect on the degradation of 4-HBC by Bacillus shenkelii QZW1.

[0083] It can be seen from the above content that the Bacillus shenkelii QZW1 provided by the present invention is capable of degrading cyano liquid crystal monomer pollutants, especially achieving efficient degradation of 4′-(hexyloxy)-4-biphenylcarbonitrile.

[0084] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A strain of Shinella sp. QZW1, deposited with GDMCC NO: 64864.

2. A bacterial agent, characterized in that The bacterial agent includes the Bacillus shenkelii QZW1 described in claim 1.

3. Use of the Bacillus shenkelii QZW1 described in claim 1 or the bacterial agent described in claim 2 in degrading cyano liquid crystal monomer pollutants; the cyano liquid crystal monomer pollutants include 4'-(hexyloxy)-4-biphenylcarbonitrile.

4. The use according to claim 3, characterized in that: The concentration of the cyano liquid crystal monomer in the cyano liquid crystal monomer contaminant is 0.25-3 mg / L.

5. A method for degrading cyano liquid crystal monomer pollutants, characterized in that: The method comprises the following steps: inoculating the Bacillus shenkelii QZW1 described in claim 1 or the bacterial agent described in claim 2 into the cyano liquid crystal monomer pollutant to be degraded, and culturing at 30° C.; The cyano liquid crystal monomer contaminant includes 4'-(hexyloxy)-4-biphenylcarbonitrile.

6. The method according to claim 5, characterized in that The initial bacterial dose OD of the Bacillus shenkelii QZW1 600 It is 0.05~0.

4.

7. The method according to claim 5, characterized in that The concentration of the cyano liquid crystal monomer in the cyano liquid crystal monomer pollutant to be degraded is 0.25-3 mg / L.

8. The method according to any one of claims 5 to 7, characterized in that: The method further comprises: adding glucose to the liquid crystal monomer pollutant to be degraded; The added amount of glucose is ≤1000 mg / L.

9. The method according to claim 8, characterized in that The added amount of glucose is 50-1000 mg / L.

10. The method according to claim 9, characterized in that The added amount of glucose is 100-500 mg / L.

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