Enterobacter holmesii and its application in preparing heavy metal remover

Abstract

The present invention discloses an Enterobacter hollandii and an application thereof in preparing a heavy metal remover. The Enterobacter hollandii ( Enterobacter hormaechei )FGS‑CS4 was deposited in Guangdong Microbial Culture Collection Center on June 15, 2023, with the deposit number GDMCC NO: 63564. The adsorption rate of copper by the bacterium can reach 75.13% in 24 hours, and the adsorption rate of lead is as high as 99.57%. Enterobacter hormaechei )FGS‑CS4 has high tolerance and adsorption rate to copper and lead, can be used to resist the stress of heavy metals, and has broad application prospects in the adsorption and removal of copper and lead.

Description

Technical Field

[0001] The invention relates to the technical field of heavy metal treatment, and in particular to Enterobacter hallii and application thereof in the preparation of a heavy metal remover. Background Art

[0002] With the rapid development of population growth and industrialization worldwide, heavy metal pollution has attracted attention due to its toxicity, bioaccumulation tendency and persistence in nature. In recent years, a large amount of heavy metals have been produced in industries such as battery preparation, mining, surface treatment, energy and fuel production, and electrical appliance manufacturing, causing serious environmental pollution and threatening human health. Due to the non-biodegradability of heavy metals, heavy metal-containing wastewater usually needs to be pretreated by various physical and chemical processes, including chemical precipitation, ion exchange, membrane separation, reverse osmosis and adsorption. Among these pretreatments, adsorption is considered to be one of the more cost-effective and environmentally friendly technologies for removing or recovering heavy metal ions in aqueous solutions. Microbial remediation of environmental heavy metal pollution has the advantages of being environmentally friendly, mild remediation process and low cost, and is the trend of heavy metal pollution control in the future.

[0003] Siderophores are low molecular weight organic compounds secreted by microorganisms under iron-restricted conditions. Their molecular weight is 200 to 2000 Da. They are highly efficient metal chelators. Their main function is to capture insoluble iron in different environments to promote the absorption of iron by microorganisms. More than 500 different types of siderophores have been discovered. In addition to being able to chelate iron ions and promote microbial growth, siderophores can also chelate other metal ions, and their affinity for different metal ions depends on the different functional groups on their surface. According to existing research, siderophores containing sulfur- and nitrogen-based metal-binding motifs have a strong affinity for Cu 2+ 、Zn 2+ and Ni 2+ It has a strong affinity for divalent ions such as cadmium and iron. Based on its strong metal chelation, microbial siderophores have been widely used in the fields of medicine, pharmaceuticals, and environmental remediation. Azmi Khan et al. extracted hydroxamic acid siderophores from Aspergillus nidulans and proved through experiments that its affinity for cadmium ions is higher than that for iron ions. The above studies show that microorganisms may have evolved to use siderophores as one of the important survival mechanisms under heavy metal stress conditions, and siderophore-producing microorganisms have shown great potential in improving heavy metal stress. Summary of the invention

[0004] In order to overcome the above defects and shortcomings in the prior art, the present invention provides an Enterobacter hallii and its application in the preparation of a heavy metal remover.

[0005] The first object of the present invention is to provide a kind of Enterobacter holmesii ( Enterobacter hormaechei )FGS-CS4.

[0006] A second object of the present invention is to provide the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the adsorption and / or removal of heavy metals.

[0007] A third object of the present invention is to provide the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in alleviating heavy metal pollution.

[0008] A fourth object of the present invention is to provide the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the treatment of heavy metal polluted water.

[0009] A fifth object of the present invention is to provide the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the treatment of heavy metal contaminated soil.

[0010] A sixth object of the present invention is to provide the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei )Application of FGS-CS4 in the preparation of heavy metal removers.

[0011] A seventh object of the present invention is to provide a heavy metal remover.

[0012] The eighth object of the present invention is to provide a method for adsorbing and / or removing heavy metals.

[0013] The ninth object of the present invention is to provide a method for alleviating heavy metal pollution.

[0014] The tenth object of the present invention is to provide a method for treating water or soil contaminated by heavy metals.

[0015] The present invention claims the following:

[0016] A type of Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 was deposited in Guangdong Provincial Microbiological Culture Collection Center on June 15, 2023, with the deposit number GDMCC NO: 63564.

[0017] The above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the adsorption and / or removal of heavy metals, wherein the heavy metals are copper and / or lead.

[0018] The above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in alleviating heavy metal pollution, wherein the heavy metal is copper and / or lead.

[0019] The above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the treatment of heavy metal contaminated water, wherein the heavy metals are copper and / or lead.

[0020] The above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the treatment of heavy metal contaminated soil, wherein the heavy metals are copper and / or lead.

[0021] The above-mentioned Enterobacter holmesii ( Enterobacter hormaechei ) Application of FGS-CS4 in the preparation of a heavy metal remover, wherein the heavy metal is copper and / or lead.

[0022] A heavy metal remover containing the above-mentioned Enterobacter holmesii ( Enterobacter hormaechei )FGS-CS4, the heavy metal is copper and / or lead.

[0023] A method for adsorbing and / or removing heavy metals, the method comprising applying the heavy metal remover, wherein the heavy metals are copper and / or lead.

[0024] A method for alleviating heavy metal pollution, the method comprising applying the heavy metal remover, wherein the heavy metal is copper and / or lead.

[0025] A method for treating water or soil polluted by heavy metals, the method comprising applying the above-mentioned heavy metal remover, wherein the heavy metals are copper and / or lead.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention discloses an Enterobacter hollandii and an application thereof in preparing a heavy metal remover. The Enterobacter hollandii ( Enterobacter hormaechei ) FGS-CS4 was deposited in Guangdong Microbiological Culture Collection Center on June 15, 2023, with the deposit number GDMCC NO: 63564, and the deposit address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The adsorption rate of copper by the bacteria can reach 75.13% in 24 hours, and the adsorption rate of lead is as high as 99.57%. The present invention's Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 has high tolerance and adsorption rate to copper and lead, can be used to resist the stress of heavy metals, and has broad application prospects in the adsorption and removal of copper and lead. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is the first isolation of siderophore-producing bacteria.

[0029] Figure 2 This is a diagram of the CAS-iron (III) plate effect of strain FGS-CS4.

[0030] Figure 3 The diagram shows the effect of strain FGS-CS4 on the chelation ability of copper and lead; A: CAS-copper (II) plate results, B: BPR-lead (II) plate results.

[0031] Figure 4 Colony morphology observation and Gram staining microscopy results of strain FGS-CS4; A: colony morphology observation, B: Gram staining microscopy results.

[0032] Figure 5 To construct a phylogenetic tree of strain FGS-CS4 based on the 16S rDNA gene sequence.

[0033] Figure 6 Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 sensitivity analysis results to copper and lead; A: Enterobacter hallii ( Enterobacter hormaechei ) The sensitivity of FGS-CS4 to copper, B: Enterobacter holmesii ( Enterobacter hormaechei ) The sensitivity of FGS-CS4 to lead, C: Enterobacter holmesii ( Enterobacter wall ) OD of FGS-CS4 in nutrient broth medium with Cu and Pb concentrations of 0 mmol / L, 2.0 mmol / L, and 3.0 mmol / L, respectively 600 nm value.

[0034] Figure 7 Enterobacter holmesii ( Enterobacter hormaechei ) SEM images and energy spectrum of FGS-CS4; A: morphology of strains without metal treatment, B: SEM morphology of strains treated with 2 mmol / L Cu, C: SEM morphology of strains treated with 2 mmol / L Pb, D: energy spectrum of strains treated with 2 mmol / L Cu, E: energy spectrum of strains treated with 2 mmol / L Pb. DETAILED DESCRIPTION

[0035] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0036] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0037] CAS-Iron (III) detection solution: Slowly add solution A into solution B along the wall of the beaker, stir and mix to obtain 100 mL of CAS blue detection solution. Solution A: weigh 0.079 g of chrome azure S (CAS) and dissolve it in 50 mL of deionized water, then add 10 mL of 1 mmol / L FeCl3 solution (prepared with HCl); Solution B: dissolve 0.069 g of hexadecyltrimethylammonium bromide (CTAB) in 40 mL of deionized water.

[0038] CAS-Copper (II) detection solution: Take 6 mL of 10 mmol / L copper sulfate solution (solvent is 10 mmol / L HCl), 6 mL of 2 mmol / L chrome azure S (CAS) solution, 12 mL of 10 mmol / L cetyltrimethylammonium bromide (CTAB) solution, 1 mL of sodium borate buffer solution (pH 6.0-6.3), 2 mL of 5% Tween 80 solution (v / v), mix thoroughly, adjust the pH to 4.0-4.3 with 0.1 mol / L NaOH and 0.1 mol / L HCl, and finally prepare a purple detection solution.

[0039] BPR-lead (II) detection solution: Take 6 mL of 10 mmol / L lead nitrate solution, 6 mL of 0.02% bromopyrogallol red (BPR) solution (w / v), 12 mL of 10 mmol / L cetyltrimethylammonium bromide (CTAB) solution, 2 mL of 5% Tween 80 solution (v / v), and 1 mL of sodium borate buffer solution (pH 6.0-6.3), mix thoroughly, and the maximum absorption wavelength of the final solution is 610 nm.

[0040] Example 1 Isolation and purification of strains

[0041] 1. Experimental Methods

[0042] The Gulf of Mexico scallop samples were collected from a heavy metal contaminated area in Suixi County, Zhanjiang City, Guangdong Province (21.8°N, 110.17°E). The surface of the Gulf of Mexico scallops was rinsed with sterile saline under sterile conditions. 10 g of the chopped Gulf of Mexico scallop intestinal sample was weighed and mixed in 90 mL of sterile saline, and a 10-fold gradient dilution was performed. 10 5 , 10 6 , 10 7 100 µL of each dilution was spread on CAS-iron (III) plates and incubated inverted at 37°C for 4 to 6 days.

[0043] After bacteria secrete iron carriers, an orange-yellow fluorescent circle will be produced around them. Colonies with more obvious fluorescent circles are selected for streaking purification until a single colony with unchanged iron carrier production capacity is obtained. The purified single colony is inoculated into nutrient broth medium for amplification to obtain a batch of strains to be tested, which are then stored in -80°C glycerol for freezing.

[0044] 2. Experimental Results

[0045] After initial separation of bacteria in the samples, a total of 50 strains of bacteria that can produce iron carriers were collected ( Figure 1). D1 represents the diameter of the fading zone, d1 represents the diameter of the colony, and the R1 ratio determines the strength of the strain's ability to produce siderophores. D1 / d1 = R1. When R1 is 1, it means that the strain has no ability to produce siderophores. The results of the CAS-iron (III) plate showed that among the 50 bacteria that can produce siderophores, the R1 value of strain FGS-CS4 was 5.48, indicating that strain FGS-CS4 has a strong ability to produce siderophores ( Figure 2 ).

[0046] Example 2 Detection of the ability of bacterial strains to chelate heavy metals

[0047] 1. Experimental Methods

[0048] The strain FGS-CS4 isolated in Example 1 was inoculated into a nutrient agar medium alone and cultured at 37°C for 4 days. After the strain secreted enough iron carriers, 10 mL of CAS-copper (II) and 10 mL of BPR-lead (II) detection liquid were poured in respectively. After standing for 1 day, the detection liquid was poured out. Finally, the plate was rinsed clean with deionized water, and the colony diameter and fading zone diameter were measured three times. The ratio (R2, R3) of the fading zone diameter (D2, D3) and the colony diameter (d2, d3) was calculated. The larger the R2 and R3, the better the ability of the strain's extracellular active substances to chelate copper and lead. The R2 and R3 values ​​of 1 indicate that the strain's extracellular active substances have no activity in chelating copper and lead.

[0049] 2. Experimental Results

[0050] The results of CAS-copper (Ⅱ) plate showed that the R2 value of strain FGS-CS4 was 7.17 ( Figure 3 A in the figure), the results of BPR-lead (Ⅱ) plate showed that the R3 value of strain FGS-CS4 was 4.05 ( Figure 3 B), indicating that the high-activity siderophore secreted by strain FGS-CS4 has a strong ability to chelate copper and lead.

[0051] Example 3 Bacterial species identification

[0052] 1. Experimental Methods

[0053] The strain FGS-CS4 isolated in Example 1 was streaked onto a nutrient agar medium, and the colonies were observed after culturing for 24 h, and Gram staining and physiological and biochemical tests were performed.

[0054] The total DNA of strain FGS-CS4 isolated in Example 1 was extracted using a bacterial genomic DNA preparation kit, and the 16S rDNA gene of strain FGS-CS4 was amplified using universal primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′, SEQ ID NO: 1) and 1492R (5′-GGTTACCTTGTTACGACTT-3′, SEQ ID NO: 2).

[0055] The total gene amplification system was 20 µL: 0.8 µL upstream primer, 0.8 µL downstream primer, 2 µL 10×PCR buffer (without MgCl2), 1.2 µL MgCl2, 0.4 µL dNTPs (10 mM), 0.4 µL Taq polymerase, 13.4 µL ddH2O, and 1 µL DNA template.

[0056] PCR reaction conditions: pre-denaturation at 95°C for 5 min; denaturation at 95°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 90 s, 35 cycles; extension at 72°C for 10 min.

[0057] The PCR amplification products were purified and sequenced for analysis. Then, the EzBioCloud program was used to obtain possible similar groups of sequenced taxa. The phylogenetic tree was constructed using the neighbor-joining method with MEGA 5 software, and the bootstrap value was 1000.

[0058] The species to which strain FGS-CS4 belongs was determined through phylogenetic tree, colony morphology analysis and physiological and biochemical tests.

[0059] 2. Experimental Results

[0060] The surface of the colony of strain FGS-CS4 is moist and smooth, with small protrusions. The colony is translucent and has neat edges. Under the microscope, the bacterial morphology is short rod-shaped, without spores, and has flagella around it. It is a Gram-negative bacterium ( Figure 4 ).

[0061] The results of physiological and biochemical tests of strain FGS-CS4 are shown in Table 1.

[0062] Table 1 Physiological and biochemical test results of FGS-CS4 strain

[0063]

[0064] Note: + indicates positive, - indicates negative.

[0065] The nucleotide sequence of the 16S rDNA gene of strain FGS-CS4 is shown in SEQ ID NO: 3. After comparison, it is similar to that of Enterobacter hallii ( Enterobacter hormaechei )DSM 16687 T The gene sequence similarity was 99.10%. The phylogenetic tree was constructed by neighbor-joining analysis, and the isolated strain FGS-CS4 belonged to Enterobacter hallii ( Enterobacter wall )( Figure 5 ).

[0066] The strain FGS-CS4 was deposited in Guangdong Microbial Culture Collection Center with the deposit number GDMCC NO: 63564. The deposit address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The preservation date is June 15, 2023. It is classified and named as Enterobacter holmesii ( Enterobacter hormaechei ).

[0067] Example 4 Enterobacter hallii ( Enterobacter hormaechei )Sensitivity analysis of FGS-CS4 to copper and lead

[0068] 1. Experimental Methods

[0069] The Enterobacter hallii of Example 3 ( Enterobacter hormaechei ) FGS-CS4 (GDMCC NO: 63564) was inoculated into nutrient broth medium and cultured at 37°C and 150 r / min for 24 h to obtain a bacterial suspension containing Enterobacter hallii ( Enterobacter hormaechei ) FGS-CS4 fermentation product. When the absorbance of the bacterial solution reached 1.000 at a wavelength of 600 nm, the solution was diluted 0, 10 1 , 10 2 , 10 3 and 10 4 times to obtain the corresponding concentration of bacterial suspension dilution.

[0070] Take 5 μL of the above five concentrations of bacterial suspension dilutions respectively and inoculate them on nutrient agar medium with Cu concentrations of 0 mmol / L, 0.5 mmol / L, 1.0 mmol / L, 1.5 mmol / L, 2.0 mmol / L, 2.5 mmol / L, 3.0 mmol / L and 3.5 mmol / L, and nutrient agar medium with Pb concentrations of 0 mmol / L, 0.5 mmol / L, 1.0 mmol / L, 1.5 mmol / L, 2.0 mmol / L, 2.5 mmol / L and 3.0 mmol / L, respectively, and culture them in a constant temperature incubator at 37°C for 24 h. Take pictures and analyze them using a gel imaging analysis system.

[0071] In addition, the above bacterial suspension was inoculated into nutrient broth medium with Cu and Pb concentrations of 0 mmol / L, 2.0 mmol / L and 3.0 mmol / L, respectively, and cultured at 37°C and 150 r / min. After 24 h, the absorbance of the bacterial solution at a wavelength of 600 nm was measured to evaluate the growth of the strain.

[0072] 2. Experimental Results

[0073] In nutrient agar medium with a Cu concentration of 0-2.5 mmol / L, Enterobacter hallii ( Enterobacter wall )FGS-CS4 grew well, indicating that 2.5 mmol / L Cu treatment had no effect on Enterobacter hallii ( Enterobacter wall )FGS-CS4 growth was affected. In the nutrient agar medium with a Cu concentration of 3.0 mmol / L, Enterobacter hallii ( Enterobacter hormaechei ) FGS-CS4 showed inhibition in growth, but still grew normally. When the Cu concentration was 3.5 mmol / L, E. Enterobacter hormaechei ) The growth of FGS-CS4 was obviously greatly inhibited, and the strain FGS-CS4 could not grow normally ( Figure 6 A in ).

[0074] In the nutrient agar medium with a Pb concentration of 0-2.0 mmol / L, Enterobacter hallii ( Enterobacter wall ) The growth of FGS-CS4 was not inhibited by Pb and it could grow normally in nutrient agar medium. When the Pb concentration increased to 2.5 mmol / L, E. Enterobacter hormaechei ) The growth of FGS-CS4 was significantly inhibited. When the Pb concentration reached 3.0 mmol / L, E. Enterobacter hormaechei ) FGS-CS4 basically did not grow. The above results show that E. Enterobacter hormaechei )FGS-CS4 has high tolerance to copper and lead( Figure 6 B in the figure).

[0075] In the absence of metal treatment, E. Enterobacter hormaechei ) FGS-CS4 grew rapidly after 24 h of culture, OD 600 nm can reach above 1.19. When the Cu concentration was 2.0 mmol / L and 3.0 mmol / L, E. Enterobacter hormaechei ) OD of FGS-CS4 at 24 h 600 nm can reach 0.95 and 0.41 respectively. In addition, when the Pb concentration is 2.0 mmol / L and 3.0 mmol / L, E. Enterobacter hormaechei ) OD of FGS-CS4 at 24 h 600 nm can reach 0.75 and 0.16 respectively ( Figure 6 C in the figure). Enterobacter holmesii ( Enterobacter hormaechei ) The Cu half-inhibitory concentration of FGS-CS4 can reach 3.0 mmol / L, and the Pb half-inhibitory concentration can reach 2.5 mmol / L. When the Pb concentration rises to 3.0 mmol / L, E. Enterobacter hormaechei ) FGS-CS4 was completely inhibited and basically did not grow.

[0076] The above results show that Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 shows strong tolerance to Cu and Pb.

[0077] Example 5 Enterobacter holmesii ( Enterobacter hormaechei ) Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis of FGS-CS4 after adsorption of Cu or Pb

[0078] 1. Experimental Methods

[0079] The Enterobacter hallii of Example 3 ( Enterobacter hormaechei )FGS-CS4 (GDMCC NO: 63564) were inoculated in nutrient broth medium with Cu or Pb concentrations of 0 and 2 mmol / L, respectively, and cultured at 37℃ and 150 r / min for 48 h. After 48 h, centrifuged at 4500 rpm for 15 min at 4℃, the supernatant was removed and the precipitate was thoroughly washed three times with phosphate buffer (pH=7.0). The bacteria were then fixed again in 2.5% glutaraldehyde solution (v / v) overnight, and then washed three times in phosphate buffer (pH=7.0). Dehydrated with ethanol gradient, freeze-dried, and observed by scanning electron microscopy with energy dispersive spectrometer.

[0080] 2. Experimental Results

[0081] Untreated E. hallii ( Enterobacter hormaechei ) The FGS-CS4 strain was rod-shaped, with a plump cell surface and no particles attached ( Figure 7 A in Figure 1). After treatment with 2 mmol / L Cu or Pb, insoluble metal particles (indicated by red arrows) appeared on the surface of E. hallii ( Enterobacter hormaechei )FGS-CS4 cell surface ( Figure 7 B and C in Figure ). After 2 mmol / L Cu or Pb treatment, E. Enterobacter hormaechei ) The presence of metal Cu and Pb elements was detected on the cell surface of FGS-CS4 ( Figure 7 D and E), indicating that Enterobacter hallii ( Enterobacter hormaechei ) The FGS-CS4 strain has a good adsorption effect on Cu and Pb.

[0082] Example 6 Enterobacter holmesii ( Enterobacter hormaechei )Adsorption rate of copper and lead by FGS-CS4

[0083] 1. Experimental Methods

[0084] The Enterobacter hallii of Example 3 ( Enterobacter hormaechei ) FGS-CS4 (GDMCC NO: 63564) was inoculated into nutrient broth and revived at 37°C and 150 rpm until OD 600 nm After recovery, Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 was inoculated into nutrient broth medium with a Cu or Pb concentration of 2 mmol / L and cultured at 37°C and 150 r / min. At 24 h, 48 h, and 72 h, the culture was centrifuged at 8000 rpm for 15 min, and the supernatant was taken. The concentrations of remaining copper and lead in the supernatant were determined by inductively coupled plasma mass spectrometry (ICP-MS), and the concentrations of Enterobacter hallii ( Enterobacter hormaechei )Adsorption rate of FGS-CS4:

[0085]

[0086] Where R is the adsorption rate; C0 is the initial Cu or Pb concentration (mmol / L); C e is the concentration of Cu or Pb in the supernatant solution.

[0087] 2. Experimental Results

[0088] The results are shown in Table 2. Enterobacter hormaechei ) The adsorption rate of copper by FGS-CS4 can reach 75% in 24 h, and the adsorption rate of lead can reach 99%.

[0089] Table 2 Enterobacter holmesii ( Enterobacter hormaechei )Adsorption rate of FGS-CS4 for Cu and Pb

[0090]

[0091] The above results show that Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4 showed a high adsorption rate for high concentration copper and lead solutions, especially lead. Enterobacter hormaechei ) FGS-CS4 or its iron carrier is prepared into a heavy metal remover, which can be used on a large scale in the remediation of copper and lead contaminated soil or water.

[0092] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A strain of Enterobacter holmesii ( Enterobacter hormaechei ) FGS-CS4, characterized in that, It was deposited in Guangdong Provincial Microbiological Culture Collection Center on June 15, 2023, with the deposit number GDMCC NO: 63564.

2. The Enterobacter holmesii of claim 1 ( Enterobacter hormaechei ) Application of FGS-CS4 in adsorption and / or removal of heavy metals, characterized in that: The heavy metal is copper and / or lead.

3. The Enterobacter holmesii of claim 1 ( Enterobacter hormaechei )The application of FGS-CS4 in alleviating heavy metal pollution is characterized by: The heavy metals are copper and / or lead.

4. The Enterobacter holmesii of claim 1 ( Enterobacter hormaechei )The application of FGS-CS4 in the treatment of heavy metal polluted water is characterized by: The heavy metals are copper and / or lead.

5. The Enterobacter holmesii of claim 1 ( Enterobacter hormaechei )The application of FGS-CS4 in the treatment of heavy metal contaminated soil is characterized by: The heavy metals are copper and / or lead.

6. The Enterobacter holmesii of claim 1 ( Enterobacter hormaechei ) Application of FGS-CS4 in the preparation of heavy metal remover, characterized in that: The heavy metal is copper and / or lead.

7. A heavy metal remover, characterized in that: Containing the Enterobacter hallii described in claim 1 ( Enterobacter hormaechei )FGS-CS4, the heavy metal is copper and / or lead.

8. A method for adsorbing and / or removing heavy metals, characterized in that: The heavy metal remover according to claim 7 is used, wherein the heavy metal is copper and / or lead.

9. A method for alleviating heavy metal pollution, characterized in that: The heavy metal remover according to claim 7 is used, wherein the heavy metal is copper and / or lead.

10. A method for treating heavy metal-contaminated water or soil, characterized in that: The heavy metal remover according to claim 7 is used, wherein the heavy metal is copper and / or lead.

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