USP gene related to drought tolerance of pomacea canaliculata, encoded protein and application of USP gene

Through the dsRNA interference technology of the USP gene in the Fushou Snail, the drought tolerance and survival rate of Fushou Snail is reduced, and the problems of diffusion and spread of Fushou Snail are solved, achieving more efficient prevention and treatment effects.

CN119709764AActive Publication Date: 2025-03-28CHINA JILIANG UNIV
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Patent Information

Application Number
CN202411890743.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-20
Publication Date
2025-03-28
Estimated Expiration
2044-12-20

AI Technical Summary

Technical Problem

The ability of Fushou snail tolerate drought causes its diffusion and spread. The existing prevention and control methods such as physical and chemical prevention have problems such as environmental pollution, high toxicity and resistance, making it difficult to effectively control its diffusion.

Method used

By extracting USP genes related to drought tolerance in Fushoushou, dsRNA was designed to interfere with RNA to inhibit the expression of USP genes, thereby reducing drought tolerance and survival of Fushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushoushous

Benefits of technology

It significantly reduces the drought tolerance and survival rate of Fushou snails, improves the prevention and treatment efficiency, and provides theoretical basis and practical reference for controlling the diffusion of Fushou snails.

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Abstract

The invention discloses a USP gene related to the drought tolerance of pomacea canaliculata, an encoded protein and application of the USP gene, and belongs to the technical field of biology. The nucleotide sequence of the USP gene is as shown in SEQ ID NO: 1; the USP gene can be used as a target for pesticide screening and biological control of ampullaria gigas. The invention further provides dsRNA of the USP gene, and the dsRNA can reduce the survival rate of ampullaria gigas and the hatching rate of ampullaria gigas eggs. The method is rapid, effective and high in repeatability, and is an important supplement to the USP gene family. The drought tolerance of the ampullaria gigas is reduced by blocking the expression of the USP gene in the ampullaria gigas, so that the purposes of controlling overwintering of the ampullaria gigas in soil in winter and reducing propagation and diffusion of the ampullaria gigas are achieved. The application disclosed by the invention has important significance on research and determination of biology, ecology and novel insecticides of the ampullaria gigas and research on a resistance mechanism of the ampullaria gigas on drought tolerance.
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