Preparation method and application of cubilose polypeptide composition

Abstract

The invention provides a cubilose polypeptide composition preparation method and application thereof, and solves the problems of low human body absorbance, much nutrition loss and the like of existing edible cubilose, and the preparation method mainly comprises the following steps: S1, mixing cubilose and deionized water according to a mass ratio of 1: 25, soaking for more than 2 hours, manually removing cubilose impurities, filtering water to obtain a cubilose sample, sampling the cubilose sample, and taking out the cubilose sample; adding pure water according to a mass ratio of 1: 25, curing at 100 DEG C or above, and preserving heat; mechanically crushing and sieving the cured cubilose sample to obtain a first slurry solution; s2, taking quantitative fish gelatin, adding 2 times of deionized water, performing high-temperature curing, and performing crushing so as to obtain a second slurry solution; s3, sampling the first pulp solution and the second pulp solution according to the mass ratio of 3: 1, mixing and stirring for more than 1 hour, and performing enzymolysis and purification treatment to obtain the corresponding polypeptide composition.

Description

Technical Field

[0001] The present invention relates to the technical field of bird's nest preparation, and in particular to a method for preparing a bird's nest polypeptide composition and application thereof. Background Art

[0002] The protein content in bird's nest accounts for a large proportion of the dry weight of bird's nest. Zhu Chunhong, Yong Wei and others tested the protein content of different bird's nests and found that the content of protein in blood bird's nests was 60.38%, yellow bird's nest was 63.56%, white bird's nest was 58.81% and bird's nest slices was 60.31%. Cha Shenghua, Jiang Shuihong and others found that the protein content of white bird's nests and blood bird's nests produced in Thailand and India was about 55-60%. Li Xiaolong and others extracted bird's nest protein by different extraction methods, among which the protein concentration of the solution obtained by water extraction method was 192.58μg / mL. Lu Yuan, Han Dengbao and others found that the protein content of bird's nests produced by Yunnan's small white-rumped swift, white-rumped swift and short-billed golden swift was 42.21%, 47.78% and 45.28% respectively.

[0003] It can be seen that bird's nests are rich in protein. Although relevant studies have confirmed that bird's nests have whitening and other related functions, their proteins are all large molecules and are difficult to be fully absorbed by the human body, indirectly causing nutrient loss. Therefore, it is of great practical significance to fully develop and utilize the small molecule peptides for absorption. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the defects of the prior art, and the present invention proposes a preparation method of a bird's nest polypeptide composition and its application, which can convert the macromolecular protein in the bird's nest into polypeptides, making it easy for the human body to absorb.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is: a method for preparing a bird's nest polypeptide composition, comprising the following steps:

[0006] S1. Mix the bird's nest with deionized water at a mass ratio of 1:25, soak for more than 2 hours, remove impurities from the bird's nest manually and filter the water to obtain a bird's nest sample, sample the bird's nest sample, add pure water at a mass ratio of 1:25, and heat-cure at 100° C. or above; mechanically crush and sieve the matured bird's nest sample to obtain a first slurry solution;

[0007] S2, take a certain amount of fish glue and add 2 times of deionized water, and crush it after high temperature aging to obtain a second slurry solution;

[0008] S3, sampling the first slurry solution and the second slurry solution at a mass ratio of 3:1, mixing and stirring for more than 1 hour, and performing enzymatic hydrolysis and purification to obtain a corresponding polypeptide composition.

[0009] Furthermore, the specific steps of the enzymatic hydrolysis process in step S3 are as follows:

[0010] Add 100 parts by weight of water and 2-3 parts by weight of complex enzyme to the mixed slurry solution, adjust the pH to 7-11, and perform enzymolysis at 45°C for 2-3 hours;

[0011] The enzymatic hydrolyzate is flocculated, and then the supernatant is freeze-dried to obtain a dry powder;

[0012] The lyophilized powder was dissolved in PBS buffer, papain and trypsin were added at the same time, and the mixture was treated at 37°C for more than 2 hours to obtain an enzymatic hydrolyzate of a polypeptide composition.

[0013] Furthermore, the complex enzyme includes one or more of α-amylase, cellulase, glucanase, pectinase and maltogenic amylase.

[0014] Furthermore, in the step S1, the mechanical crushing method includes one or a combination of colloid mill crushing, high-speed shear crushing equipment crushing, and homogenization crushing.

[0015] Furthermore, the pressure of the high-pressure homogenizer and microfluidizer homogenization is 5-60 MPa, and the number of homogenization times is no less than 3 times.

[0016] Furthermore, during the immersion process in step S1, the immersion temperature is 20-45°C; and during the soaking, 0.01wt%-1.0wt% sodium bicarbonate aqueous solution or vitamin C aqueous solution is used.

[0017] Furthermore, in step S2, the specific preparation steps of the second slurry solution are as follows:

[0018] Take 10g dried fish maw, wash it, put it into a pelletizer to cut into strips and pellets, sieve it, put it into a high-speed grinder to grind it into fish gelatin pellets,

[0019] Add fish glue particles into water at a solid-liquid ratio of 1:30 and soak them. After the fish glue is fully swollen, treat them in a 121°C water bath for 10 minutes to obtain fish glue homogenate;

[0020] Add composite protease to the fish glue homogenate at a mass ratio of 3:1, perform enzymolysis at 50° C. for 4 hours, and then adjust the pH to neutral to obtain the corresponding second slurry solution.

[0021] The application of the preparation method of the bird's nest polypeptide composition as described above in the preparation of anti-aging and whitening cosmetics.

[0022] The application of the preparation method of the bird's nest polypeptide composition as described above in food.

[0023] Compared with the prior art, the beneficial effects of the present invention include: polypeptide glue with different physical and chemical properties and functions can be obtained by enzymatically hydrolyzing the pretreated fish glue with different proteases, and then the hydrolyzate after enzymatic hydrolysis is flocculated, and then the supernatant is freeze-dried to obtain a dry powder; the freeze-dried powder is dissolved in PBS buffer, and papain and trypsin are added at the same time, and treated at 37°C for more than 2 hours to obtain a polypeptide composition-type hydrolyzate, thereby realizing the polypeptideization of the original large molecular protein in the bird's nest, which is convenient for further absorption by the human body. DETAILED DESCRIPTION

[0024] It is easy to understand that according to the technical solution of the present invention, without changing the essential spirit of the present invention, a person skilled in the art can propose a variety of interchangeable structural modes and implementation modes. Therefore, the following specific implementation modes and drawings are only exemplary descriptions of the technical solution of the present invention, and should not be regarded as the whole of the present invention or as a limitation or restriction to the technical solution of the present invention.

[0025] As for the overall steps, in this embodiment, a method for preparing a bird's nest polypeptide composition comprises the following steps:

[0026] S1. Mix the bird's nest with deionized water at a mass ratio of 1:25, soak for more than 2 hours, remove impurities from the bird's nest manually and filter the water to obtain a bird's nest sample, sample the bird's nest sample, add pure water at a mass ratio of 1:25, and heat-cure at 100° C. or above; mechanically crush and sieve the matured bird's nest sample to obtain a first slurry solution;

[0027] S2, take a certain amount of fish glue and add 2 times of deionized water, and crush it after high temperature aging to obtain a second slurry solution;

[0028] S3, sampling the first slurry solution and the second slurry solution at a mass ratio of 3:1, mixing and stirring for more than 1 hour, and performing enzymatic hydrolysis and purification to obtain a corresponding polypeptide composition.

[0029] Furthermore, the specific steps of the enzymatic hydrolysis process in step S3 are as follows:

[0030] Add 100 parts by weight of water and 2-3 parts by weight of complex enzyme to the mixed slurry solution, adjust the pH to 7-11, and perform enzymolysis at 45°C for 2-3 hours;

[0031] The enzymatic hydrolyzate is flocculated, and then the supernatant is freeze-dried to obtain a dry powder;

[0032] The lyophilized powder was dissolved in PBS buffer, papain and trypsin were added at the same time, and the mixture was treated at 37°C for more than 2 hours to obtain an enzymatic hydrolyzate of a polypeptide composition.

[0033] Furthermore, the complex enzyme includes one or more of α-amylase, cellulase, glucanase, pectinase and maltogenic amylase.

[0034] Furthermore, in the step S1, the mechanical crushing method includes one or a combination of colloid mill crushing, high-speed shear crushing equipment crushing, and homogenization crushing.

[0035] Furthermore, the pressure of the high-pressure homogenizer and microfluidizer homogenization is 5-60 MPa, and the number of homogenization times is no less than 3 times.

[0036] Furthermore, during the immersion process in step S1, the immersion temperature is 20-45°C; and during the soaking, 0.01wt%-1.0wt% sodium bicarbonate aqueous solution or vitamin C aqueous solution is used.

[0037] Furthermore, in step S2, the specific preparation steps of the second slurry solution are as follows:

[0038] Take 10g dried fish maw, wash it, put it into a pelletizer to cut into strips and pellets, sieve it, put it into a high-speed grinder to grind it into fish gelatin pellets,

[0039] Add fish glue particles into water at a solid-liquid ratio of 1:30 and soak them. After the fish glue is fully swollen, treat them in a 121°C water bath for 10 minutes to obtain fish glue homogenate;

[0040] Add composite protease to the fish glue homogenate at a mass ratio of 3:1, perform enzymolysis at 50° C. for 4 hours, and then adjust the pH to neutral to obtain the corresponding second slurry solution.

[0041] The application of the preparation method of the bird's nest polypeptide composition as described above in the preparation of anti-aging and whitening cosmetics.

[0042] The application of the preparation method of the bird's nest polypeptide composition as described above in food.

[0043] The bird's nest sampling process includes: firstly taking the dry cave bird's nest, selecting and removing the bird's feathers and other impurities, and setting aside for use; (2) soaking the clean bird's nest in pure water for 16 hours, tearing the bird's nest into small strips, and adding the strips into pure water 20 times the weight of the bird's nest; (3) heating the bird's nest obtained in step (2) at 115°C and keeping it warm for 0.7 hours, at which time the bird's nest is dissolved into a liquid state; (4) filtering the liquid bird's nest to obtain a filtrate; (5) vacuum freeze-drying the filtrate at 70°C and 30 Pa to obtain a freeze-dried bird's nest with a water content of 2%; (6) crushing the freeze-dried bird's nest obtained in step (5), and passing it through a 500-mesh sieve to obtain an instant bird's nest product.

[0044] In summary, the preparation method of the polypeptide composition provided in the embodiment of the present invention can obtain polypeptide glue with different physical and chemical properties and functions by enzymolysis of pre-treated fish glue with different proteases, and then perform subsequent treatment with a protease compounded with acidic protease and pepsin under acidic treatment conditions. The hydrolyzate after enzymolysis is flocculated, and then the supernatant is freeze-dried to obtain a dry powder; the freeze-dried powder is dissolved in PBS buffer, and papain and trypsin are added at the same time, and the hydrolyzate is treated at 37°C for more than 2 hours to obtain a polypeptide composition-type hydrolyzate, thereby realizing the polypeptideization of the original macromolecular protein in the bird's nest, which is convenient for further absorption by the human body.

[0045] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms should not be understood as necessarily being directed to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification.

[0046] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A method for preparing a bird's nest polypeptide composition, characterized in that: The steps include: S1. Mix the bird's nest with deionized water at a mass ratio of 1:25, soak for more than 2 hours, remove impurities from the bird's nest manually and filter the water to obtain a bird's nest sample, sample the bird's nest sample, add pure water at a mass ratio of 1:25, and heat-cure at 100° C. or above; mechanically crush and sieve the matured bird's nest sample to obtain a first slurry solution; S2, take a certain amount of fish glue and add 2 times of deionized water, and crush it after high temperature aging to obtain a second slurry solution; S3, sampling the first slurry solution and the second slurry solution at a mass ratio of 3:1, mixing and stirring for more than 1 hour, and performing enzymatic hydrolysis and purification to obtain a corresponding polypeptide composition.

2. The method for preparing a bird's nest polypeptide composition according to claim 1, characterized in that: The specific steps of the enzymatic hydrolysis process in step S3 are as follows: Add 100 parts by weight of water and 2-3 parts by weight of complex enzyme to the mixed slurry solution, adjust the pH to 7-11, and perform enzymolysis at 45°C for 2-3 hours; The enzymatic hydrolyzate is flocculated, and then the supernatant is freeze-dried to obtain a dry powder; The lyophilized powder was dissolved in PBS buffer, papain and trypsin were added at the same time, and the mixture was treated at 37°C for more than 2 hours to obtain an enzymatic hydrolyzate of a polypeptide composition.

3. The method for preparing a bird's nest polypeptide composition according to claim 2, characterized in that: The complex enzyme comprises one or more of α-amylase, cellulase, glucanase, pectinase and maltogenic amylase.

4. The method for preparing a bird's nest polypeptide composition according to claim 1, characterized in that: In the step S1, the mechanical crushing method includes one or a combination of colloid mill crushing, high-speed shear crushing equipment crushing, and homogenization crushing.

5. The method for preparing a bird's nest polypeptide composition according to claim 4, characterized in that: The pressure of the high-pressure homogenizer and micro-jet homogenizer is 5-60MPa, and the homogenization times are no less than 3 times.

6. The method for preparing a bird's nest polypeptide composition according to claim 1, characterized in that: During the immersion process in step S1, the immersion temperature is 20-45° C.; during the soaking, 0.01wt%-1.0wt% sodium bicarbonate aqueous solution or vitamin C aqueous solution is used.

7. The method for preparing a bird's nest polypeptide composition according to claim 1, characterized in that: In step S2, the specific steps of preparing the second slurry solution are as follows: Take 10g dried fish maw, wash it, put it into a pelletizer to cut into strips and pellets, sieve it, put it into a high-speed grinder to grind it into fish gelatin pellets, Add fish glue particles into water at a solid-liquid ratio of 1:30 and soak them. After the fish glue is fully swollen, treat them in a 121°C water bath for 10 minutes to obtain fish glue homogenate; Add composite protease to the fish glue homogenate at a mass ratio of 3:1, perform enzymolysis at 50° C. for 4 hours, and then adjust the pH to neutral to obtain the corresponding second slurry solution.

8. Application of the preparation method of the bird's nest polypeptide composition according to any one of claims 1 to 7 in the preparation of anti-aging and whitening cosmetics.

9. Application of the method for preparing the bird's nest polypeptide composition according to any one of claims 1 to 7 in food.