Food safety detection workstation and control system and control method thereof

Abstract

The invention relates to the technical field of food safety detection, in particular to a food safety detection workstation and a control system and method thereof, and the food safety detection workstation comprises a detection table, and a pretreatment module and a detection module which are arranged on the detection table; the pretreatment module is used for carrying out various pretreatment operations on a to-be-detected sample according to a required flow and providing a reaction solution of the pretreated to-be-detected sample for the detection module; the detection module comprises a third detection module, and the third detection module comprises a reagent placement frame, a microporous strip placement channel, a reagent liquid adding module, a plate washing mechanism, a tip suction box mechanism, a tip suction removal mechanism, a reading module and a third mechanical arm; the device can continuously load samples and process a plurality of samples at the same time, so that the detection efficiency is improved, the detection period is shortened, and the test error is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of food safety detection, and in particular to a food safety detection workstation and a control system and a control method thereof. Background Art

[0002] As consumers' demands for food quality increase and food safety incidents increase, the public is paying more and more attention to food safety, and the monitoring of food quality and safety is receiving more and more attention.

[0003] Among the several existing food safety testing equipment, some are small and portable and can be used for on-site testing, but they often require manual operation and cannot achieve automated testing. In addition, since they are used for on-site testing, they are greatly affected by the environment and it is difficult to avoid sample contamination problems. Some testing equipment that can achieve automated experimental operations usually have preset processes for rapid test items and can only complete the operation of one test item at a time. Existing food safety testing equipment is usually an analytical instrument based on fluorescent test cards, which usually requires manual operation to load the test cards, increasing the risk of human error and resulting in low detection throughput, which in turn leads to low detection efficiency and large experimental errors.

[0004] Therefore, there is an urgent need for a food safety testing workstation and its control system and control method, which can continuously load samples without interruption, process multiple samples at the same time, improve testing efficiency, shorten testing cycle, and reduce test errors. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a food safety testing workstation and its control system and control method, which can continuously load samples without interruption and process multiple samples at the same time, thereby improving testing efficiency, shortening testing cycle and reducing experimental errors.

[0006] The present invention provides a food safety detection workstation, comprising: A testing platform, and a pre-processing module and a testing module arranged on the testing platform; The pre-treatment module is used to pre-treat the sample to obtain a solution to be detected by the detection module; The detection module includes a third detection module, which includes a reagent placement rack, a microporous strip placement channel, a reagent liquid addition module, a plate washing mechanism, a tip box mechanism, a tip removal mechanism, a reading module and a third mechanical arm; the microporous strip placement channel is used to place the enzyme-labeled micropores, the reagent placement rack is arranged above the microporous strip placement channel, and is used to place the reagents required for detection, the reagent liquid addition module is arranged between the microporous strip placement channel and the reagent placement rack, and is used to add reagents to the enzyme-labeled micropores to react with the solution to be detected, and the plate washing mechanism is arranged at one end of the microporous strip placement channel close to the edge of the detection table, and is used to wash the enzyme The microwell is marked, the tip box mechanism and the tip removal mechanism are arranged above the microwell strip placement channel and side by side on one side of the reagent placement rack. The tip box mechanism is used to store the tip to replace the needle of the liquid needle on the third mechanical arm, and the tip removal mechanism is used to remove the used needle. The reading module is arranged between the microwell strip placement channel and the reagent placement rack, and is used to measure the result of the solution to be detected after the reaction in the enzyme-labeled microwell. The third mechanical arm is used to absorb the lower layer of liquid from the centrifuge tube containing the reaction liquid in the pre-treatment module into the enzyme-labeled microwell or absorb the reagent from the reagent in the reagent placement rack into the enzyme-labeled microwell.

[0007] Further, the pre-processing module includes a first processing module and a second processing module; The first processing module includes a sample loading channel, a front liquid adding module, a sample transfer mechanism, a first oscillator, a first centrifuge and a first mechanical arm; the sample loading channel is used to place the sample tube, the sample transfer mechanism is used to move the sample tube in the sample loading channel to the front liquid adding module or the second processing module, the front liquid adding module is arranged on one side of the sample transfer mechanism so that the sample tube supported by the sample transfer mechanism can be moved to the needle position corresponding to the front liquid adding module, and the first mechanical arm is used to transfer the sample tube to the first oscillator, the first centrifuge or the sample transfer mechanism; The second processing module includes a waiting turntable and a second robotic arm; the waiting turntable is used to place centrifuge tubes, and the second robotic arm is used to absorb supernatant from the sample tubes in the sample transfer mechanism into the centrifuge tubes, and transfer the centrifuge tubes to the waiting turntable.

[0008] Furthermore, the first processing module also includes an incubation system, and the first robotic arm is further used to transfer the sample tube into the incubation system; The second processing module also includes a second centrifuge, a second centrifuge balancing mechanism, a second oscillator, an air blowing system, a post-liquid addition module and a liquid needle cleaning mechanism; the second centrifuge balancing mechanism is used to balance the second centrifuge, the air blowing system is used to concentrate the sample pretreatment extract, the post-liquid addition module is used to add liquid to the centrifuge tube placed on the turntable of the post-liquid addition module, the liquid needle cleaning mechanism is arranged at the middle position between the end of the sample transfer mechanism and the air blowing system, and is used to clean the liquid needle arranged on the second robotic arm, and the second robotic arm is also used to transfer the centrifuge tube to the second centrifuge, the second oscillator, the air blowing system or the post-liquid addition module.

[0009] Further, the first mechanical arm is a U-shaped structure, including two longitudinal beams, a cross beam and a clamping claw, the two longitudinal beams are provided with slide rails, the two ends of the cross beam are respectively fixed on the slide rails of the two longitudinal beams, the cross beam is provided with a slide rail, the clamping claw is fixed on the slide rail of the cross beam, the cross beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the clamping claw can slide left and right along the direction of the cross beam through the slide rail of the cross beam; The second robot arm is an L-shaped structure, including a longitudinal beam, a transverse beam, a clamping claw and a liquid needle. A slide rail is arranged on the transverse beam, one end of the longitudinal beam is fixed on the slide rail of the transverse beam, and the slide rail is arranged under the longitudinal beam. The clamping claw and the liquid needle are fixed on the slide rail of the longitudinal beam. The liquid needle is arranged on a side close to the transverse beam, and the clamping claw is arranged on a side away from the transverse beam. The longitudinal beam can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam, and the clamping claw and the liquid needle can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam. The third robotic arm is an L-shaped structure, including a longitudinal beam, a transverse beam and a liquid needle. A slide rail is provided on the longitudinal beam, one end of the transverse beam is fixed on the slide rail of the longitudinal beam, and the slide rail is under the transverse beam. The liquid needle is fixed on the slide rail of the transverse beam. The transverse beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the liquid needle can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam.

[0010] Furthermore, a liquid level detection system is provided at the liquid needle of the second robotic arm for detecting the liquid level in the sample tube so that the liquid needle and the liquid surface are always at a minimum effective contact distance.

[0011] Furthermore, the air blowing system includes two air blowing modules, each air blowing module includes 6 air blowing positions and 6 air blowing waiting positions, the air blowing positions and the air blowing waiting positions are arranged in two rows on the turntable of the air blowing module, and the air blowing positions and the air blowing waiting positions are switched by rotating the turntable.

[0012] Furthermore, the air blowing pipe of the air blowing module descends in sections according to parameter settings, and always maintains a minimum effective air blowing distance from the liquid surface in the sample tube.

[0013] The present invention also provides a control system for a food safety testing workstation, which is implemented based on the above-mentioned food safety testing workstation and includes: The data analysis module is used to create a test project before the experiment begins, and to establish a standard curve for the test project by scanning the standard curve QR code on the corresponding project kit or performing a standard curve experiment; it is also used to generate a data analysis report based on the experimental results after the experiment is completed, and to provide a printed report; The experimental operation module is used to set up experiments, including making standard curve experiments. According to the system prompts, place the standard curve making reagents at the corresponding positions on the food safety testing workstation. After placing the reagents, the equipment automatically enters the production of the corresponding product standard curve and obtains the standard curve data of the project. After receiving the standard curve data, the system automatically saves the standard curve data of the corresponding name. It is also used to perform sample testing experiments. First, scan the sample code and load it, set the number of sample tubes in a set of loading racks and the preset test process to be executed, and when the sample loading is completed, the equipment automatically executes the testing process.

[0014] Furthermore, it also includes: A pipette tip setting module is used to set the starting position of the pipette tip before the experiment starts; The liquid circuit maintenance module is used to drain the front liquid circuit, the rear liquid circuit, the AB liquid and the cleaning liquid containing reagents before the experiment begins, and drain the waste liquid in the corresponding reagent bottle into the drain tank; The process detection module is used to track the status of each module of the equipment in real time during the experiment; The equipment pause module is used to pause the equipment process when it is necessary to add various liquid reagents or deal with abnormal situations. When the equipment is paused, the timing of each module in operation will also be paused; A first centrifuge alarm module, used for giving an alarm when the first centrifuge is not trimmed, and the first centrifuge automatically stops working; The first centrifuge abnormality processing module is used to, when the first centrifuge is not trimmed, cause the sample tube currently in the first centrifuge to be withdrawn so that the device can continue to work normally; A centrifuge emptying module is used to empty the centrifuge when a test tube remains in the first centrifuge or the second centrifuge due to equipment failure, power failure or component damage; The air blowing abnormality processing module is used to perform abnormal processing when the air blowing needle cannot fall normally, so that the equipment can continue the air blowing process; The sample rack abnormality processing module is used to prompt the staff to remove all samples on the sample rack and reload them when the sample rack is abnormal due to equipment failure, power failure or component damage; The enzyme label abnormality processing module is used to perform abnormal processing when the enzyme label strip carrier of the microwell strip placement channel is stuck when entering or exiting the channel, and to drag out the stuck enzyme label strip carrier.

[0015] The present invention also provides a control method for a food safety testing workstation, which is implemented based on the above-mentioned food safety testing workstation or a control system of a food safety testing workstation, and includes: S1, replenish the cleaning solution for the pipette tip, centrifuge tube and the second robotic arm’s dosing needle; S2, drain the front liquid adding liquid path, the rear liquid adding liquid path, the AB liquid and the cleaning liquid, and drain the waste liquid in the corresponding reagent bottle into the drain tank; S3. Create a test project, and establish a standard curve for the test project by scanning the standard curve QR code on the corresponding project kit or performing a standard curve experiment; S4. Execute the sample detection experiment. First, scan the sample code and load it. Set the number of sample tubes in a sample loading rack and the preset test process to be executed. When the sample loading is completed, the equipment automatically executes the detection process. S5. After the testing process is completed, a data analysis report is generated based on the experimental results and a printed report is provided.

[0016] The embodiments of the present invention have the following technical effects: The fully automatic food safety fluorescence detection and analysis workstation designed by the present invention integrates a variety of modules and functions, and can automatically complete all steps from sample pre-processing and post-detection to data report generation. The modules in the system are arranged according to the test process to form a smooth work chain, which ensures the effective connection and efficient operation of each step. The first, second, and third robotic arms are used to realize automatic transfer of samples, which reduces manual intervention and improves work efficiency. The system can continuously load samples without interruption and can process multiple samples at the same time, which greatly improves the detection efficiency, shortens the detection cycle, and reduces test errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a schematic diagram of the overall structure of a food safety testing workstation provided by an embodiment of the present invention; Figure 2 It is a structural schematic diagram of a food safety testing workstation provided by an embodiment of the present invention; Figure 3 It is a structural schematic diagram of a mechanical arm of a food safety testing workstation provided by an embodiment of the present invention; Figure 4 The present invention provides a flowchart of a method for controlling a food safety testing workstation.

[0019] In the figure: 1-first processing module; 11-loading channel; 12-front liquid addition module; 13-incubation system; 14-sample transfer mechanism; 15-first oscillator; 16-first centrifuge; 17-first robotic arm; 2-second processing module; 21-second centrifuge; 22-second centrifuge balancing mechanism; 23-second oscillator; 24-air blowing system; 25-rear liquid addition module; 26-liquid needle cleaning mechanism; 27-waiting turntable; 28-second robotic arm; 3-third detection module; 31-reagent display rack; 32-microwell strip display channel; 33-plate washing mechanism; 34-tip box mechanism; 35-tip removal mechanism; 36-third robotic arm; 4-detection table. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0021] The embodiment of the present invention provides a food safety detection workstation. Figure 1 is a schematic diagram of the overall structure of a food safety testing workstation provided by an embodiment of the present invention. Figure 2 is a structural schematic diagram of a food safety testing workstation provided by an embodiment of the present invention, Figure 3 is a schematic diagram of the structure of a mechanical arm of a food safety testing workstation provided by an embodiment of the present invention, see Figure 1-Figure 3 , specifically comprising: a detection platform 4, and a pre-processing module and a detection module arranged on the detection platform 4; The pre-treatment module is used to pre-treat the sample to obtain a solution to be detected by the detection module; The detection module includes a third detection module 3, which includes a reagent rack 31, a microporous strip placement channel 32, a reagent liquid adding module (not shown in the figure, in this embodiment, the reagent liquid adding module is located below the reagent rack 31), a plate washing mechanism 33, a tip box mechanism 34, a tip removal mechanism 35, a reading module (not shown in the figure, in this embodiment, the reagent liquid adding module is located below the reagent rack 31) and a third robotic arm 36; the microporous strip placement channel 32 is used to place enzyme-labeled microwells, the reagent placement rack 31 is arranged above the microporous strip placement channel 32, and is used to place reagents required for detection, and the reagent liquid adding module is arranged between the microporous strip placement channel 32 and the reagent placement rack 31, and is used to add reagents to the enzyme-labeled microwells, react with the solution to be detected, and wash. The plate mechanism 33 is arranged at one end of the microporous strip placement channel 32 close to the edge of the detection platform 4, and is used to clean the enzyme-labeled micropores. The tip box mechanism 34 and the tip removal mechanism 35 are arranged above the microporous strip placement channel 32 and are arranged side by side on one side of the reagent placement rack 31. The tip box mechanism 34 is used to store the tip to replace the needle of the liquid needle on the third mechanical arm 36. The tip removal mechanism 35 is used to remove the used needle. The reading module is arranged between the microporous strip placement channel 32 and the reagent placement rack 31, and is used to measure the result of the solution to be detected after the reaction in the enzyme-labeled micropores. The third mechanical arm 36 is used to absorb the lower layer of liquid from the centrifuge tube containing the reaction liquid in the pre-treatment module into the enzyme-labeled micropores or absorb the reagent from the reagent in the reagent placement rack 31 into the enzyme-labeled micropores.

[0022] In some embodiments, the pre-processing module includes a first processing module 1 and a second processing module 2; The first processing module 1 includes a sample loading channel 11, a pre-liquidation module 12, an incubation system 13, a sample transfer mechanism 14, a first oscillator 15, a first centrifuge 16 and a first mechanical arm 17; the sample loading channel 11 is used to place sample tubes, the sample transfer mechanism 14 is used to move the sample tubes in the sample loading channel 11 to the pre-liquidation module 12 or the second processing module, the pre-liquidation module 12 is arranged on one side of the sample transfer mechanism 14 so that the sample tubes supported by the sample transfer mechanism 14 can be moved to the needle position corresponding to the pre-liquidation module 12, and the first mechanical arm 17 is used to transfer the sample tubes to the incubation system 13, the first oscillator 15, the first centrifuge 16 or the sample transfer mechanism 14; The second processing module 2 includes a second centrifuge 21, a second centrifuge balancing mechanism 22, a second oscillator 23, an air blowing system 24, a post-liquid adding module 25, a liquid needle cleaning mechanism 26, a waiting turntable 27 and a second robotic arm 28; the second centrifuge balancing mechanism 22 is used to balance the second centrifuge 21, the air blowing system 24 is used to concentrate the sample pre-treatment extract, the post-liquid adding module 25 is used to add liquid to the centrifuge tube placed on the turntable of the post-liquid adding module 25, and the liquid needle cleaning mechanism 26 is used to clean the sample. 6 is arranged in the middle position between the end of the sample transfer mechanism 14 and the air blowing system 24, so that the action distance is relatively short and most reasonable, the liquid needle cleaning mechanism 26 is used to clean the liquid needle arranged on the second mechanical arm 28, the waiting turntable 27 is used to place the sample tube and the centrifuge tube, and the second mechanical arm 28 is used to absorb the supernatant from the sample tube in the sample transfer mechanism 14 into the centrifuge tube, and transfer the centrifuge tube to the second centrifuge 21, the second oscillator 23, the air blowing system 24, the post-liquid addition module 25 or the waiting turntable 27.

[0023] In some embodiments, the structure of the food safety testing workstation is laid out and positioned based on the module motion trajectory, physical avoidance, and process trajectory. Some customized position settings can also be made based on customization requirements and the different products used in each module.

[0024] The first mechanical arm 17 is a U-shaped structure, including two longitudinal beams, a cross beam and a clamping claw. The two longitudinal beams are provided with slide rails, and the two ends of the cross beam are respectively fixed on the slide rails of the two longitudinal beams. The cross beam is provided with a slide rail, and the clamping claw is fixed on the slide rail of the cross beam. The cross beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the clamping claw can slide left and right along the direction of the cross beam through the slide rail of the cross beam. The second mechanical arm 28 is an L-shaped structure, including a longitudinal beam, a transverse beam, a clamping claw and a liquid needle. A slide rail is arranged on the transverse beam, one end of the longitudinal beam is fixed on the slide rail of the transverse beam, and the slide rail is arranged under the longitudinal beam. The clamping claw and the liquid needle are fixed on the slide rail of the longitudinal beam. The liquid needle is arranged on a side close to the transverse beam, and the clamping claw is arranged on a side away from the transverse beam. The longitudinal beam can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam, and the clamping claw and the liquid needle can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam. The third robot arm 36 is an L-shaped structure, including a longitudinal beam, a transverse beam and a liquid needle. A slide rail is arranged on the longitudinal beam, one end of the transverse beam is fixed on the slide rail of the longitudinal beam, and the slide rail is arranged under the transverse beam. The liquid needle is fixed on the slide rail of the transverse beam. The transverse beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the liquid needle can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam.

[0025] In some embodiments, the first robotic arm 17, the second robotic arm 28 and the third robotic arm 36 are all X, Y, and Z three-axis robotic arms, which are respectively used to transfer sample tubes, centrifuge tubes, and sample liquids, wherein the first robotic arm 17 is used to realize the transfer of sample tubes between various functional modules; the second robotic arm 28 is used to realize the transfer of centrifuge tubes and supernatant between various functional modules; the third robotic arm 36 is used to realize the transfer operation of the reaction liquid in the enzyme labeling experiment link and the dripping operation of the reaction liquid in the colloidal gold experiment link.

[0026] In some embodiments, the loading channel 11 is used to transport the sample tube from the loading channel 11 to the loading position for placing, confirming and recovering the sample. The sample tube is placed on the loading carrier for transportation. The loading channel 11 is equipped with 5 groups of loading carriers, each group of loading carriers is used to place samples of the same detection target, and each group of loading carriers is equipped with 6 sample positions, which can realize the simultaneous detection of 30 samples at a time. When a loading carrier returns to the initial loading position, the system will automatically prompt the user to continue the loading operation, thereby realizing uninterrupted continuous loading.

[0027] In some embodiments, the sample transfer mechanism 14 is used to move a sample tube loaded with a sample within the device.

[0028] In some embodiments, the pre-liquid adding module 12 is used to add pre-treatment liquid to the sample. Various pre-treatment liquids are assembled according to the liquid path distribution table to complete the sample pre-treatment liquid adding step. In some embodiments, the food safety testing workstation can be equipped with 21 sets of single-channel liquid circuits, including 13 sets of pre-treatment liquid circuits and 8 sets of post-treatment liquid circuits (or classified according to 5 sets of organic reagent liquid circuits, 14 sets of conventional reagent liquid circuits and 2 sets of microliter reagent liquid circuits). Each reagent has its own liquid circuit to ensure the accuracy of liquid addition.

[0029] In some embodiments, the incubation system 13 is used for high-temperature incubation of sample pretreatment. The incubation system 13 is equipped with 12 tube positions, which can meet the simultaneous heating and incubation of 12 sample tubes. The incubation system 13 is also provided with an intelligent temperature control module, which can achieve temperature adjustment of 0-120 degrees Celsius, and the temperature control accuracy can reach ±2 degrees Celsius.

[0030] In some embodiments, the first centrifuge 16 is equipped with 6 tube positions for centrifugal separation of samples. When the number of sample tubes is 1 or 5, sample tubes of equal volume and weight need to be placed on the sample loading rack or the balancing position of the large centrifuge to balance the first centrifuge 16.

[0031] In some embodiments, the second centrifuge 21 is equipped with 6 tube positions for centrifuging centrifuge tubes.

[0032] In some embodiments, the first oscillator 15 is used for sample pretreatment extraction oscillation. The first oscillator 15 is configured with 6 tube positions and adopts "pendulum-type" oscillation, which can make the sample to be tested collide violently with the tube wall in the sample tube. It can also be used for sample grinding in conjunction with grinding beads to fully break the sample, so that the extraction effect is better and the time is shortened. When oscillating the liquid, it can also have a vortex mixing motion effect.

[0033] In some embodiments, the second oscillator 23 is used to mix the liquid in the centrifuge tube, and the second oscillator 23 is configured with 6 tube positions.

[0034] In some embodiments, the air blowing system 24 is used to concentrate the sample pretreatment extract. The air blowing system 24 includes two air blowing modules, each of which includes 6 air blowing positions and 6 air blowing waiting positions, that is, it can simultaneously meet the needs of 12 samples being air blown at the same time and 12 samples being waited. The air blowing positions and the air blowing waiting positions are arranged in two rows on the turntable of the air blowing module. The air blowing positions and the air blowing waiting positions are switched by rotating the turntable, which greatly improves the turnover efficiency of the air blowing positions and can effectively alleviate the reduction in experimental efficiency caused by the long air blowing time. Among them, the air blowing pipe of the air blowing system 24 is provided with auxiliary hot air, which can accelerate the working efficiency of the air blowing link. The air blowing pipe descends in sections and always maintains the minimum effective air blowing distance with the liquid level in the centrifuge tube, further improving the air blowing efficiency.

[0035] In some embodiments, a liquid level detection system is also provided at the liquid needle of the second mechanical arm 28, which is used to detect the liquid level in the centrifuge tube, and is equipped with a capacitive liquid level detection sensor to make the liquid level detection more accurate. Corresponding parameters are set according to different liquids, so that the liquid needle and the liquid surface are always at the minimum effective contact distance, to prevent the liquid needle from carrying liquid droplets and causing inaccurate liquid volume, and also effectively prevent the liquid needle from contaminating the reagent.

[0036] In some embodiments, the liquid needle cleaning mechanism 26 is equipped with two sets of needle washing systems for cleaning the liquid needle of the second robotic arm 28. It adopts the "fountain-type" liquid needle cleaning technology, which can effectively clean the outer wall and inner cavity of the liquid needle and achieve zero crossover and zero contamination between different samples.

[0037] In some embodiments, the waiting turntable 27 is used for switching centrifuge tubes during sample pre-treatment and for waiting for the placement of centrifuge tubes to be tested. The waiting turntable 27 is equipped with 48 tube positions.

[0038] In some embodiments, the microporous strip placement channel 32 is equipped with 20 × 8 tube enzyme label strip carriers, which can simultaneously perform experimental tests on 160 tubes of sample liquid to be tested. The enzyme label tube carrier passes through the reading module at high speed. The reading module is a single probe detection module, which can solve the interpretation error caused by different reaction times between different samples and the problem of inconsistent interpretation of results by multiple sets of probes, thereby increasing the accuracy and consistency of the result interpretation. The reading module selects a filter with an excitation light of 370nm and a filter with a receiving light of 525nm.

[0039] The fully automatic food safety fluorescence detection and analysis workstation designed by the present invention integrates a variety of modules and functions, and can automatically complete all steps from sample pre-processing and post-detection to data report generation. The modules in the system are arranged according to the test process to form a smooth work chain, which ensures the effective connection and efficient operation of each step. The first, second, and third robotic arms are used to realize automatic transfer of samples, which reduces manual intervention and improves work efficiency. The system can continuously load samples without interruption and can process multiple samples at the same time, which greatly improves the detection efficiency, shortens the detection cycle, and reduces test errors.

[0040] The embodiment of the present invention further provides a control system for a food safety testing workstation, which is implemented based on the above-mentioned food safety testing workstation and specifically includes the following modules: A pipette tip setting module is used to set the starting position of the pipette tip before the experiment starts; The liquid circuit maintenance module is used to drain the front liquid circuit, the rear liquid circuit, the AB liquid and the cleaning liquid containing reagents before the experiment begins, and drain the waste liquid in the corresponding reagent bottle into the drain tank; Data analysis module, used to create test projects and establish standard curves for test projects; project names and reagent-related parameters can be directly obtained by scanning the QR code on the corresponding project kit, and standard product curves can be obtained by directly scanning the standard product curve QR code on the kit, or by conducting a standard product curve experiment; The experiment operation module is used to set up the experiment, including making a standard curve experiment. According to the system prompt, place the standard curve making reagent at the corresponding position on the food safety testing workstation. After placing the reagent, the equipment automatically enters the production of the corresponding product standard curve and obtains the standard curve data of the project. After receiving the standard curve data, the system automatically saves the standard curve data of the corresponding name; In some embodiments, the validity period of the standard product curve data is calculated from the day the data is received. When the standard product curve data expires, the standard product curve data will be marked in red. Only one copy of the standard product curve data of the same project can be saved. When the standard product curve data of the project is received again, a prompt will be displayed to overwrite the old data. If the overwriting is confirmed, the validity period of the standard product curve data of the project will also be updated to the date of the new data. The experimental operation module is also used to perform sample detection experiments. First, the sample code is scanned and loaded, and the number of sample tubes in a set of loading racks and the preset test process to be executed are set. When the sample loading is completed, the equipment automatically executes the detection process; In some embodiments, when some experimental items can be combined with pre-processing, the pre-processing combined processing can also be performed; when the status bar of the sample loading channel 11 displays a green light, it means that the channel is idle and the sample can be loaded; when the status bar of the sample loading channel 11 displays a red light, it means that the channel is occupied, and the sample cannot be loaded even if the channel is currently vacant; In some embodiments, it is also possible to set whether to carry quality control products together with the detection experiment as needed. If you choose to carry quality control products together with the detection experiment, the system will display a quality control placement prompt, and the quality control products are placed according to the prompt. At the same time, the microporous strip also needs to increase the quality control hole position; The data analysis module is also used to generate data analysis reports based on experimental results and provide printed reports; In some embodiments, you can choose to generate a data analysis report based on a single set or multiple sets of data, and the format of the report can be set by checking a preset display item; The process detection module is used to track the status of each module of the equipment in real time during the experiment; Equipment pause module, used to pause the equipment process when it is necessary to add various liquid reagents or deal with abnormal situations; When the device is paused, the timing of each running module will also be paused; A first centrifuge alarm module, used to alarm when the first centrifuge 16 is not trimmed, and the first centrifuge 16 automatically stops working; The first centrifuge abnormality processing module is used to, when the first centrifuge 16 is not trimmed, make the sample tube currently in the first centrifuge 16 exit the operation so that the device can continue to work normally; It should be noted that after the abnormality of the first centrifuge 16 is handled, it is necessary to reload the sample, and the current experiment of this group of samples is discarded; A centrifuge emptying module is used to empty the centrifuge when a test tube is left in the first centrifuge 16 or the second centrifuge 21 due to equipment failure, power failure or component damage; The air blowing abnormality processing module is used to perform abnormal processing when the air blowing needle cannot fall normally, so that the equipment can continue the air blowing process; The sample rack abnormality processing module is used to prompt the staff to remove all samples on the sample rack and reload them when the sample rack is abnormal due to equipment failure, power failure or component damage; The enzyme label abnormality processing module is used to perform abnormal processing when the enzyme label strip carrier of the microporous strip placement channel 32 is stuck in the inlet and outlet channel, and drag out the stuck enzyme label strip carrier; It should be noted that after the abnormal enzyme labeling is handled, the sample needs to be reloaded and the current experiment of this group of samples will be discarded.

[0041] This embodiment provides a control method for a food safety testing workstation, which is implemented based on the above-mentioned food safety testing workstation or a control system of a food safety testing workstation. Figure 4 is a flow chart of a control method of a food safety testing workstation provided by an embodiment of the present invention, see Figure 4 , including the following steps: S1, replenishing the cleaning solution of the pipette tip, centrifuge tube and the second robot arm 28; S2, drain the front liquid adding liquid path, the rear liquid adding liquid path, the AB liquid and the cleaning liquid, and drain the waste liquid in the corresponding reagent bottle into the drain tank; S3. Create a test project, and establish a standard curve for the test project by scanning the standard curve QR code on the corresponding project kit or performing a standard curve experiment; S4. Execute the sample detection experiment. First, scan the sample code and load it. Set the number of sample tubes in a sample loading rack and the preset test process to be executed. When the sample loading is completed, the equipment automatically executes the detection process. S5. After the testing process is completed, a data analysis report is generated based on the experimental results and a printed report is provided.

[0042] In some embodiments, the detection process of the food safety detection workstation described in S4 includes the following steps: S41. Pre-process the sample.

[0043] Specifically include: S41.1. The X-axis and Y-axis of the sample transfer mechanism 14 cooperate with each other to move the sample loading carrier left and right and front and back to the corresponding needle position of the front liquid adding module 12. A maximum of 3 reagents can be added at a time, and multiple additions can be made according to the experimental process requirements; S41.2, transferring the sample tube to the first oscillator 15 through the first mechanical arm 17 to perform an oscillation operation; S41.3, transferring the sample tube to the incubation system 13 by the first robotic arm 17 to perform an incubation operation; S41.4, the sample tube is transferred to the sample loading rack by the first robot arm 17 and returned to the initial position for rest; S41.5, transferring the sample tube to the first centrifuge 16 through the first mechanical arm 17 to perform centrifugation operation; S41.6, the centrifuge tube on the waiting turntable 27 is transferred to the air blowing position of the air blowing system 24 by the gripper of the second robot arm 28; S41.7, the sample tube is transferred to the supernatant placement position on the sample transfer mechanism 14 (the supernatant placement position is the sample tube placement rack on the transverse slide rail of the sample transfer mechanism 14) by the first robot arm 17, the supernatant placement position is moved transversely along the slide rail to the end, and the supernatant in the sample tube is taken by the liquid needle of the second robot arm 28 and transferred to the centrifuge tube in the air blowing system 24; S41.8. After the supernatant is taken, the supernatant placement position moves laterally along the slide rail to the starting end, and the sample tube is transferred back to the sample loading rack by the first robot arm 17; S41.9, the sample loading rack returns to the initial position; S41.10, the air blowing position of the air blowing system 24 rotates to dry the supernatant in the centrifuge tube for concentration; S41.11, the centrifuge tube is transferred to the rear liquid adding module 25 by the gripper of the second robot 28 for liquid adding, and the liquid adding turntable of the rear liquid adding module 25 rotates so that the centrifuge tube corresponds to the corresponding needle position for liquid adding; S41.12, transfer the centrifuge tube to the second oscillator 23 through the gripper of the second robot arm 28 to perform an oscillation operation; S41.13, transfer the centrifuge tube to the second centrifuge 21 through the gripper of the second robot arm 28 to perform centrifugation operation; S41.14, the centrifuge tube is transferred back to the waiting turntable 27 by the gripper of the second robot arm 28.

[0044] S42. Perform post-test on the sample.

[0045] Specifically include: S42.1, when the waiting turntable 27 rotates to the target position, the third mechanical arm 36 moves to the waiting turntable 27 to absorb the middle and lower layers of liquid in the centrifuge tube and transfer them to the enzyme-labeled microwells; S42.2, after the third mechanical arm 36 sucks the liquid in the centrifuge tube on the waiting turntable 27 in sequence, it moves to the reagent position to suck the "standard point" reagent and transfers the liquid to the enzyme-labeled microwell; S42.3, the enzyme binding reagent (located in the reagent placement rack 31) is sucked by the third mechanical arm 36, and transferred to the enzyme-labeled microwells in sequence; S42.4, the antibody reagent (located in the reagent rack 31) is sucked up by the third mechanical arm 36, and transferred to the enzyme-labeled microwells in sequence; S42.5, the base motion mechanism of the microwell strip placement channel 32 moves to the bottom of the enzyme-labeled microwell carrier and performs reciprocating motion to mix the reagents added to the enzyme-labeled microwells; S42.6. The base movement mechanism of the microwell strip placement channel 32 moves the enzyme-labeled microwell carrier to the incubation chamber below the reagent placement rack 31 for incubation and timing; S42.7, when the timing ends, the base movement mechanism of the microwell strip placement channel 32 moves the enzyme-labeled microwell carrier to the initial position; S42.8, the plate washing mechanism 33 moves to the top of the enzyme labeling strip carrier to perform the plate washing operation, and then descends to the inside of the enzyme labeling microwell to absorb the liquid; S42.9, after the plate washer 33 has completed the liquid aspiration, it rises, and the base movement mechanism of the microporous strip placement channel 32 moves the enzyme labeling strip carrier, and sequentially aligns the hole positions of the enzyme labeling micropores with the liquid adding needles of the plate washer 33 to add liquid; S42.10, after the liquid addition is completed, the enzyme label strip carrier returns to the initial position; S42.11, the plate washing mechanism 33 descends to the inside of the enzyme-labeled microwell to absorb the liquid, and the actions of S2.9-S2.11 are repeated 4 times; S42.12. The base movement mechanism of the microwell strip placement channel 32 moves the enzyme label strip carrier to the bottom of the reading module, and adds liquid through the reagent adding module; S42.13, after the liquid addition is completed, the base motion mechanism of the microporous strip placement channel 32 reciprocates to mix the added reagents; S42.14, the base movement mechanism of the microporous strip placement channel 32 moves the enzyme label strip carrier to the incubation chamber located below the reagent placement rack 31 for incubation and timing; S42.15, after the timing is over, the base movement mechanism of the microporous strip placement channel 32 moves the enzyme label strip carrier to the initial position and performs reciprocating motion to mix the liquid after the reaction again; S42.16. The base motion mechanism of the microwell strip placement channel 32 moves the enzyme label strip carrier to the bottom of the reading module for data reading.

[0046] The above operations are the complete experimental steps of the food safety workstation. The steps can be deleted according to the needs of different detection objects. For different detection objects, the time and number of times required for each step are different. Each step is repeated according to the preset process of different detection objects. For example, after executing S41.1 for the first group of samples and entering S41.2, S41.1 is executed on the second group of samples at the same time, and so on. This achieves continuous detection of multiple groups of detection objects and improves detection throughput.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the technical solutions of the embodiments of the present invention.

Claims

1. A food safety testing workstation, characterized in that: include: A testing platform (4), and a pre-processing module and a testing module arranged on the testing platform (4); The pre-treatment module is used to pre-treat the sample to obtain a solution to be detected by the detection module; The detection module comprises a third detection module (3), and the third detection module (3) comprises a reagent placement rack (31), a microporous strip placement channel (32), a reagent liquid addition module, a plate washing mechanism (33), a tip box mechanism (34), a tip removal mechanism (35), a reading module and a third mechanical arm (36); the microporous strip placement channel (32) is used to place enzyme-labeled microporous holes, the reagent placement rack (31) is arranged above the microporous strip placement channel (32) and is used to place reagents required for detection, the reagent liquid addition module is arranged between the microporous strip placement channel (32) and the reagent placement rack (31) and is used to add reagents to the enzyme-labeled microporous holes to react with the solution to be detected, and the plate washing mechanism (33) is arranged at the microporous strip placement channel (32) near the edge of the detection platform (4). The tip box mechanism (34) and the tip removal mechanism (35) are arranged above the microwell strip placement channel (32) and are arranged side by side on one side of the reagent placement rack (31). The tip box mechanism (34) is used to store the tip to replace the needle of the liquid needle on the third mechanical arm (36). The tip removal mechanism (35) is used to remove the used needle. The reading module is arranged between the microwell strip placement channel (32) and the reagent placement rack (31) and is used to measure the result of the solution to be detected after the reaction in the enzyme-labeled microwell. The third mechanical arm (36) is used to absorb the lower layer of liquid from the centrifuge tube containing the reaction liquid in the pre-treatment module into the enzyme-labeled microwell or absorb the reagent from the reagent in the reagent placement rack (31) into the enzyme-labeled microwell.

2. A food safety testing workstation according to claim 1, characterized in that: The pre-processing module comprises a first processing module (1) and a second processing module (2); The first processing module (1) comprises a sample loading channel (11), a front liquid adding module (12), a sample transfer mechanism (14), a first oscillator (15), a first centrifuge (16) and a first mechanical arm (17); the sample loading channel (11) is used for placing a sample tube; the sample transfer mechanism (14) is used for moving the sample tube in the sample loading channel (11) to the front liquid adding module (12) or the second processing module (2); the front liquid adding module (12) is arranged on one side of the sample transfer mechanism (14) so ​​that the sample tube supported by the sample transfer mechanism (14) can be moved to a needle position corresponding to the front liquid adding module (12); and the first mechanical arm (17) is used for transferring the sample tube to the first oscillator (15), the first centrifuge (16) or the sample transfer mechanism (14); The second processing module (2) comprises a waiting turntable (27) and a second robotic arm (28); the waiting turntable (27) is used to place centrifuge tubes, and the second robotic arm (28) is used to absorb supernatant from the sample tubes in the sample transfer mechanism (14) into the centrifuge tubes, and to transfer the centrifuge tubes to the waiting turntable (27).

3. A food safety testing workstation according to claim 2, characterized in that: The first processing module (1) further comprises an incubation system (13), and the first robotic arm (17) is further used to transfer the sample tube into the incubation system (13); The second processing module (2) further comprises a second centrifuge (21), a second centrifuge balancing mechanism (22), a second oscillator (23), an air blowing system (24), a post-liquid addition module (25) and a liquid needle cleaning mechanism (26); the second centrifuge balancing mechanism (22) is used to perform a balancing operation on the second centrifuge (21); the air blowing system (24) is used to perform a concentration operation on the sample pre-treatment extract; the post-liquid addition module (25) is used to perform a liquid addition operation on a centrifuge tube placed on a turntable of the post-liquid addition module (25); the liquid needle cleaning mechanism (26) is arranged at an intermediate position between the end of the sample transfer mechanism (14) and the air blowing system (24) and is used to clean a liquid needle arranged on a second mechanical arm (28); the second mechanical arm (28) is also used to transfer the centrifuge tube to the second centrifuge (21), the second oscillator (23), the air blowing system (24) or the post-liquid addition module (25).

4. A food safety testing workstation according to claim 2, characterized in that: The first mechanical arm (17) is a U-shaped structure, comprising two longitudinal beams, a transverse beam and a clamping claw, the two longitudinal beams are provided with a slide rail, the two ends of the transverse beam are respectively fixed on the slide rails of the two longitudinal beams, the transverse beam is provided with a slide rail, the clamping claw is fixed on the slide rail of the transverse beam, the transverse beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the clamping claw can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam; The second mechanical arm (28) is an L-shaped structure, comprising a longitudinal beam, a transverse beam, a clamping claw and a liquid needle, a slide rail is arranged on the transverse beam, one end of the longitudinal beam is fixed on the slide rail of the transverse beam, the slide rail is arranged below the longitudinal beam, the clamping claw and the liquid needle are fixed on the slide rail of the longitudinal beam, the liquid needle is arranged on a side close to the transverse beam, and the clamping claw is arranged on a side away from the transverse beam, the longitudinal beam can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam, and the clamping claw and the liquid needle can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam; The third mechanical arm (36) is an L-shaped structure, comprising a longitudinal beam, a transverse beam and a liquid needle, wherein a slide rail is arranged on the longitudinal beam, one end of the transverse beam is fixed on the slide rail of the longitudinal beam, the slide rail is arranged below the transverse beam, and the liquid needle is fixed on the slide rail of the transverse beam, and the transverse beam can slide forward and backward along the direction of the longitudinal beam through the slide rail of the longitudinal beam, and the liquid needle can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam.

5. A food safety testing workstation according to claim 4, characterized in that: A liquid level detection system is also provided at the liquid needle of the second mechanical arm (28) for detecting the height of the liquid level in the sample tube so that the liquid needle and the liquid level are always at a minimum effective contact distance.

6. A food safety testing workstation according to claim 3, characterized in that: The air blowing system (24) comprises two air blowing modules, each of which comprises 6 air blowing positions and 6 air blowing waiting positions, the air blowing positions and the air blowing waiting positions being arranged in two rows on a turntable of the air blowing module, and the air blowing positions and the air blowing waiting positions being switched by rotating the turntable.

7. A food safety testing workstation according to claim 6, characterized in that: The air blowing pipe of the air blowing module descends in sections according to parameter settings, and always maintains a minimum effective air blowing distance with the liquid surface in the sample tube.

8. A control system for a food safety testing workstation, implemented based on a food safety testing workstation according to any one of claims 1 to 7, characterized in that: include: The data analysis module is used to create a test project before the experiment begins, and to establish a standard curve for the test project by scanning the standard curve QR code on the corresponding project kit or performing a standard curve experiment; it is also used to generate a data analysis report based on the experimental results after the experiment is completed, and to provide a printed report; The experimental operation module is used to set up experiments, including making standard curve experiments. According to the system prompts, place the standard curve making reagents at the corresponding positions on the food safety testing workstation. After placing the reagents, the equipment automatically enters the production of the corresponding product standard curve and obtains the standard curve data of the project. After receiving the standard curve data, the system automatically saves the standard curve data of the corresponding name. It is also used to perform sample testing experiments. First, scan the sample code and load it, set the number of sample tubes in a set of loading racks and the preset test process to be executed, and when the sample loading is completed, the equipment automatically executes the testing process.

9. A control system for a food safety testing workstation according to claim 8, characterized in that: Also includes: A pipette tip setting module is used to set the starting position of the pipette tip before the experiment starts; The liquid circuit maintenance module is used to drain the front liquid circuit, the rear liquid circuit, the AB liquid and the cleaning liquid containing reagents before the experiment begins, and drain the waste liquid in the corresponding reagent bottle into the drain tank; The process detection module is used to track the status of each module of the equipment in real time during the experiment; The equipment pause module is used to pause the equipment process when it is necessary to add various liquid reagents or deal with abnormal situations. When the equipment is paused, the timing of each module in operation will also be paused; A first centrifuge alarm module, used for giving an alarm when the first centrifuge is not trimmed, and the first centrifuge automatically stops working; The first centrifuge abnormality processing module is used to, when the first centrifuge is not trimmed, cause the sample tube currently in the first centrifuge to be withdrawn so that the device can continue to work normally; A centrifuge emptying module is used to empty the centrifuge when a test tube remains in the first centrifuge or the second centrifuge due to equipment failure, power failure or component damage; The air blowing abnormality processing module is used to perform abnormal processing when the air blowing needle cannot fall normally, so that the equipment can continue the air blowing process; The sample rack abnormality processing module is used to prompt the staff to remove all samples on the sample rack and reload them when the sample rack is abnormal due to equipment failure, power failure or component damage; The enzyme label abnormality processing module is used to perform abnormal processing when the enzyme label strip carrier of the microwell strip placement channel is stuck when entering or exiting the channel, and to drag out the stuck enzyme label strip carrier.

10. A control method for a food safety testing workstation, implemented based on the control system of a food safety testing workstation according to any one of claims 1 to 7 or a food safety testing workstation according to any one of claims 8 to 9, characterized in that: The steps include: S1, replenish the cleaning solution for the pipette tip, centrifuge tube and the second robotic arm’s dosing needle; S2, drain the front liquid adding liquid path, the rear liquid adding liquid path, the AB liquid and the cleaning liquid, and drain the waste liquid in the corresponding reagent bottle into the drain tank; S3. Create a test project, and establish a standard curve for the test project by scanning the standard curve QR code on the corresponding project kit or performing a standard curve experiment; S4. Execute the sample detection experiment. First, scan the sample code and load it. Set the number of sample tubes in a sample loading rack and the preset test process to be executed. When the sample loading is completed, the equipment automatically executes the detection process. S5. After the testing process is completed, a data analysis report is generated based on the experimental results and a printed report is provided.

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