Ion analysis method based on bilinear ion trap mass spectrometer

Abstract

The invention relates to the technical field of mass spectrometer analysis, in particular to an ion analysis method based on a bilinear ion trap mass spectrometer, and the method comprises the steps: introducing ions of a to-be-detected sample into a first linear ion trap of the bilinear ion trap mass spectrometer; target ions in a first linear ion trap are selected, the first linear ion trap transmits the target ions to a second linear ion trap of the bilinear ion trap mass spectrometer in multiple times, and the second linear ion trap carries out tandem mass spectrometry analysis on the ions transmitted each time; and identifying the complete ion structure of the to-be-detected sample according to the tandem mass spectrometry analysis data of the ions transmitted each time. Therefore, the problems that in the prior art, when a traditional mass spectrum analysis method is used for treating an extremely trace sample, the number of sample injection times is limited, and only one specific type of analysis can be performed during sample injection each time, so that the types of ions identified by a complete structure are few and the like are solved.

Description

Technical Field

[0001] The present application relates to the technical field of mass spectrometry instrument analysis, and in particular to an ion analysis method based on a dual linear ion trap mass spectrometer. Background Art

[0002] The mass spectrometer has become an indispensable tool in the field of analytical chemistry due to its extremely high sensitivity. It can accurately determine the mass-to-charge ratio (m / z) of ions. There are many types of mass analyzers, the core components of mass spectrometers, including sector magnetic field, Fourier transform ion cyclotron resonance, orbital ion trap, time of flight, quadrupole and linear ion trap. In order to deeply analyze the structure of unknown molecules in complex samples, tandem mass spectrometry (MS n ) technology has been developed. By combining multiple mass analyzers, hybrid mass spectrometers have been developed to achieve multiple mass spectrometry analysis modes. Take the triple quadrupole mass spectrometer as an example, which is composed of three quadrupoles in series. Among them, the first and third quadrupoles are used to select specific ions, and the middle quadrupole can fragment the ions, thereby achieving multiple analysis modes such as fragment ion scanning, neutral loss scanning and parent ion scanning.

[0003] The linear ion trap is a mass analyzer with unique advantages. It can store ions and improve ion utilization. In addition, it is easy to realize MS n Analysis function. The linear ion trap can use sine waves or rectangular waves to achieve ion trapping and excitation. The latter is also called a digital linear ion trap. The movement of ions in the ion trap satisfies the Mathieu equation. When the ion is in the stable region, it will move stably in the trap; when the ion is outside the stable region, the motion trajectory will be unstable. By introducing auxiliary signals, unstable points or regions can be introduced in the stable region, thereby achieving the selection of ions with specific mass-to-charge ratios and collision-induced fragmentation in the trap. In addition, the linear ion trap has the advantages of simple structure and the ability to operate under higher vacuum conditions, and is widely used in small mass spectrometers. A dual linear ion trap mass spectrometer has been developed, and fragment ion scanning and parent ion scanning modes have been implemented on the device.

[0004] However, when analyzing trace samples, such as single cells, it is not possible to perform MS analysis on multiple biomolecules due to the limitation of injection times. n This limits the comprehensive analysis of complex biomolecular structures. For example, to identify the sn-position and C=C position of the fatty acid chain on a phospholipid molecule, multiple injections are often required to perform MS on the target ions separately. 2 ,MS 3 and MS 4 Currently, the number of injections is limited and each injection can only perform one specific type of analysis, resulting in fewer ions for complete structural identification. Summary of the invention

[0005] The present application provides an ion analysis method based on a dual linear ion trap mass spectrometer and a dual linear ion trap mass spectrometer to solve the problem that when traditional mass spectrometry analysis methods in related technologies process extremely trace samples, the number of injections is limited and only one specific type of analysis can be performed each time, resulting in a small number of ion types for complete structural identification.

[0006] A first aspect of the present application provides an ion analysis method based on a dual linear ion trap mass spectrometer, comprising the following steps: introducing ions of a sample to be tested into a first linear ion trap of the dual linear ion trap mass spectrometer; selecting target ions in the first linear ion trap, wherein the first linear ion trap transmits the target ions to a second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, and the second linear ion trap performs tandem mass spectrometry analysis on each transmitted ion; and identifying the complete ion structure of the sample to be tested based on the tandem mass spectrometry analysis data of each transmitted ion.

[0007] Optionally, the first linear ion trap controls the ratio and mass-to-charge ratio of ions transmitted each time based on the first auxiliary signal.

[0008] Optionally, the first auxiliary signal controls the mass-to-charge ratio of ions transmitted by the first linear ion trap by adjusting the frequency.

[0009] Optionally, the first auxiliary signal controls the ratio of ions transmitted by the first linear ion trap by adjusting the amplitude.

[0010] Optionally, the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, including: transmitting ions of a target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer according to a target ratio; or, transmitting ions of a target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times according to the target ratio, or, simultaneously transmitting ions of different mass-to-charge ratios to the second linear ion trap of the dual linear ion trap mass spectrometer according to different ratios, or, simultaneously transmitting ions of different mass-to-charge ratios to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times according to the target ratio.

[0011] Optionally, the sum of the transmission ratios of ions of one mass-to-charge ratio in the multiple transmissions does not exceed 100%, and the sum of the transmission ratios of each type of ions of different mass-to-charge ratios in the multiple transmissions does not exceed 100%.

[0012] Optionally, the second linear ion trap realizes ion excitation based on a second auxiliary signal to perform tandem mass spectrometry analysis on the ions transmitted each time.

[0013] Optionally, the tandem mass spectrometry analysis includes MS 2 Analysis, MS3 Analysis or MS 4 analyze.

[0014] Optionally, the first auxiliary signal and the second auxiliary signal are sinusoidal waves or rectangular waves.

[0015] A second aspect of the present application provides a dual linear ion trap mass spectrometer, comprising: a first linear ion trap and a second linear ion trap; a controller, for controlling the first linear ion trap to transmit the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times according to a first auxiliary signal, performing tandem mass spectrometry analysis on each transmitted ion according to the second auxiliary signal, and identifying the complete ion structure of the sample to be tested based on the tandem mass spectrometry analysis data of each transmitted ion.

[0016] Therefore, this application has at least the following beneficial effects:

[0017] The embodiment of the present application can introduce ions of the sample to be tested into the first linear ion trap of the dual linear ion trap mass spectrometer; select target ions in the first linear ion trap, wherein the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, and the second linear ion trap performs tandem mass spectrometry analysis on each transmitted ion; identify the complete ion structure of the sample to be tested based on the tandem mass spectrometry analysis data of each transmitted ion; through the method of multiple analyses after a single injection, in-depth identification of multiple target ion structures can be achieved, and multiple MS analyses can be performed on multiple target ions without increasing the number of injections. n Analysis, thereby increasing the number of ions for complete structural identification; obtaining multiple MS images in a short time n Spectra accelerate the analysis process, enhance the ability to resolve structures, and have high applicability. They can be applied to the analysis of extremely trace samples to achieve in-depth identification of the structures of a variety of substances.

[0018] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which:

[0020] Figure 1 A flowchart of an ion analysis method based on a dual linear ion trap mass spectrometer provided according to an embodiment of the present application;

[0021] Figure 2 A schematic diagram of parameters of the Mathieu equation followed by the movement of ions in a linear ion trap according to an embodiment of the present application;

[0022] Figure 3 A primary mass spectrogram with different transmission ratios provided according to an embodiment of the present application;

[0023] Figure 4 is a curve diagram showing the relationship between the ion transmission ratio and the amplitude of the first auxiliary signal provided according to an embodiment of the present application;

[0024] Figure 5 An analysis flow chart provided according to an embodiment of the present application;

[0025] Figure 6 According to the complete structure identification process of phospholipid molecules provided in the examples of this application, MS n Schematic diagram of mass spectrum and identification results;

[0026] Figure 7 An analysis flow chart provided according to another embodiment of the present application;

[0027] Figure 8 A schematic diagram of the transmission mass-to-charge ratio range, ratio and number of times provided according to an embodiment of the present application;

[0028] Fig. 9 A block diagram of a dual linear ion trap mass spectrometer provided according to an embodiment of the present application;

[0029] Fig.10 A schematic diagram of a dual linear ion trap device for a mass spectrometer provided according to an embodiment of the present application;

[0030] Fig.11 1 is a cross-sectional view of a first linear ion trap and a second linear ion trap provided according to an embodiment of the present application.

[0031] Explanation of the reference numerals: dual linear ion trap mass spectrometer 10 , first linear ion trap 101 , second linear ion trap 102 , controller 106 , first auxiliary signal 201 , second auxiliary signal 202 , first gate electrode 103 , second gate electrode 104 , third gate electrode 105 . DETAILED DESCRIPTION

[0032] Embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

[0033] The following describes an ion analysis method based on a dual linear ion trap mass spectrometer and a dual linear ion trap mass spectrometer according to an embodiment of the present application with reference to the accompanying drawings.

[0034] Specifically, Figure 1A schematic flow chart of an ion analysis method based on a dual linear ion trap mass spectrometer provided in an embodiment of the present application.

[0035] like Figure 1 As shown, the ion analysis method based on the dual linear ion trap mass spectrometer includes the following steps:

[0036] In step S101, ions of a sample to be tested are introduced into a first linear ion trap of a dual linear ion trap mass spectrometer.

[0037] It can be understood that the embodiment of the present application can introduce ions of the sample to be tested into the first linear ion trap of the dual linear ion trap mass spectrometer to effectively store a large number of ions using the first linear ion trap, which allows multiple analyses to be performed after a single injection, thereby improving sample utilization.

[0038] It should be noted that the first linear ion trap mainly stores target ions, and selects part of the target ions to be transmitted to the second linear ion trap.

[0039] In step S102, target ions are selected in the first linear ion trap, wherein the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, and the second linear ion trap performs tandem mass spectrometry on each transmitted ion.

[0040] Among them, Fig.11 As shown, the first auxiliary signal and the second auxiliary signal are sinusoidal waves or rectangular waves, 201 and 202 are both sinusoidal wave signals, and 101 and 102 are driven by sinusoidal waves.

[0041] It can be understood that the embodiment of the present application can transmit the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times through the first linear ion trap, and the second linear ion trap performs tandem mass spectrometry on each transmitted ion. Multiple analyses after a single injection reduce the sample loss caused by multiple injections, and multiple transmissions allow multiple MS to be performed on the same target ion. n Analysis can obtain more detailed structural information, improve sample utilization and structural identification integrity.

[0042] It should be noted that the movement of ions in the ion trap follows the Mathieu equation, and its parameter diagram is shown in the figure below: Figure 2 As shown, where a and q are proportional to f represents the frequency of the inherent radio frequency electric field of the ion trap; the intersection of the curve and the q axis is 0.908; the area enclosed by the curve and the q axis is the stable motion area of ​​ions; for a linear ion trap, the a value is 0, so the stable motion area of ​​ions in the linear ion trap is between q values ​​0 and 0.908.

[0043] By introducing an auxiliary signal into the linear ion trap, an unstable point can be added to the q-axis; when an ion is at an unstable point, ion ejection or ion fragmentation will occur, and the position of the unstable point is only related to the frequency of the auxiliary signal.

[0044] In the embodiment of the present application, the first linear ion trap controls the ratio and mass-to-charge ratio of ions transmitted each time based on the first auxiliary signal.

[0045] The first auxiliary signal controls the first linear ion trap by adjusting the frequency, and the first auxiliary signal controls the ratio of ion transmission of the first linear ion trap by adjusting the amplitude.

[0046] It can be understood that the first linear ion trap of the embodiment of the present application controls the ratio and mass-to-charge ratio of ions transmitted each time based on the first auxiliary signal. By adjusting the frequency of the first auxiliary signal, ions with a specific mass-to-charge ratio can be accurately selected; this enables the analysis process to focus on the ions of interest, thereby improving the pertinence and accuracy of the analysis; by adjusting the amplitude of the first auxiliary signal, the number or ratio of ions transmitted each time can be controlled. This flexibility allows the transmission ratio to be adjusted according to experimental requirements, thereby optimizing subsequent MS n Analysis; it not only improves sample utilization, but also enhances the ability to analyze complex molecular structures.

[0047] Specifically, for the dual linear ion trap device, the target ions or ions within the mass-to-charge ratio range can be selected to be transmitted from the first linear ion trap 101 to the second linear ion trap 102 by adjusting the frequency of the first auxiliary signal 201. By adjusting the amplitude of the first auxiliary signal 201, different transmission ratios can be adjusted. The transmission results using the standard sample (mass-to-charge ratio, 782) are shown as follows: Figure 3 shown.

[0048] Figure 3 The transmission ratios corresponding to (1), (2), and (3) are 10%, 30%, and 80%, respectively, and the corresponding amplitudes of the first auxiliary signal 201 are 152mV, 176mV, and 236mV, respectively. Using this sample, the relationship curve between the transmission ratio and the amplitude of the first auxiliary signal 201 is measured as follows: Figure 4 shown.

[0049] In an embodiment of the present application, the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, including: transmitting ions of a target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer according to a target ratio; or, transmitting ions of a target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times according to the target ratio, or, transmitting ions of different mass-to-charge ratios to the second linear ion trap of the dual linear ion trap mass spectrometer at the same time according to different ratios, or, transmitting ions of different mass-to-charge ratios to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times according to the target ratio.

[0050] The sum of the ratios of multiple transmissions of ions of one mass-to-charge ratio does not exceed 100%, and the sum of the ratios of multiple transmissions of ions of different mass-to-charge ratios does not exceed 100%.

[0051] It can be understood that the embodiment of the present application can adopt multiple operating modes to transfer target ions to the second linear ion trap of the dual linear ion trap mass spectrometer. This operating mode provides a high degree of flexibility and precise control capability, which not only improves the selectivity and sensitivity of the analysis, enhances the quality and reliability of the data, but also can effectively utilize the sample, especially when processing extremely trace samples. It has significant advantages.

[0052] In the embodiment of the present application, the second linear ion trap realizes the excitation of ions based on the second auxiliary signal to perform tandem mass spectrometry analysis on each transmitted ion.

[0053] Among them, tandem mass spectrometry includes MS 2 Analysis, MS 3 Analysis or MS 4 analyze.

[0054] It can be understood that the second linear ion trap of the embodiment of the present application realizes ion excitation based on the second auxiliary signal to perform tandem mass spectrometry analysis on each transmitted ion, thereby realizing efficient multi-stage mass spectrometry analysis and deeply analyzing the structure of complex molecules.

[0055] It should be noted that the second linear ion trap performs multiple different MS n Analysis can obtain serial analysis data in different dimensions, thereby completely identifying the structure of complex molecules.

[0056] In step S103, the complete ion structure of the sample to be tested is identified based on the tandem mass spectrometry analysis data of each ion transmission.

[0057] It can be understood that the embodiments of the present application can identify the complete structures of multiple ions based on the tandem mass spectrometry analysis data of each ion transmission, accelerate the analysis process, enhance the structural analysis capability, and have high applicability.

[0058] According to the ion analysis method based on a dual linear ion trap mass spectrometer proposed in an embodiment of the present application, ions of the sample to be tested are introduced into the first linear ion trap of the dual linear ion trap mass spectrometer; target ions in the first linear ion trap are selected, wherein the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, and the second linear ion trap performs tandem mass spectrometry analysis on each transmitted ion; the complete ion structure of the sample to be tested is identified based on the tandem mass spectrometry analysis data of each transmitted ion; through the method of multiple analyses after a single injection, in-depth identification of multiple target ion structures can be achieved, and multiple MS analyses can be performed on multiple target ions without increasing the number of injections. n Analysis, thereby increasing the number of ions for complete structural identification; obtaining multiple MS images in a short time n Spectra accelerate the analysis process, enhance the ability to resolve structures, and have high applicability. They can be applied to the analysis of extremely trace samples to achieve in-depth identification of the structures of a variety of substances.

[0059] The ion analysis method based on the dual linear ion trap mass spectrometer of the present application will be described in detail below in conjunction with specific embodiments, as follows:

[0060] Example 1: Figure 5 As shown, the embodiment of the present application takes the in-depth identification of the target phospholipid molecular structure in a single cell as an example, introduces the phospholipid ions into the first linear ion trap of the dual linear ion trap mass spectrometer, and after the injection, the ions enter the first linear ion trap 101 and are stored, and then enter the cyclic analysis until all the target ions are analyzed. In a single cycle, the first linear ion trap 101 transfers the target ions of a specific mass-to-charge ratio to the second linear ion trap 102 three times, and the transmission ratio each time is 30%, ensuring that the total transmission ratio of the target ions does not exceed 100%. The setting of the single transmission ratio is achieved by adjusting the amplitude of the first auxiliary signal 201. The second linear ion trap performs MS on the target ions transmitted three times, respectively. 2 Analysis, MS 3 Analysis and MS 4 It is worth noting that the first linear ion trap 101 retransmits ions to the second linear ion trap 102 , and the previous ion analysis needs to be completed in the second linear ion trap 102 .

[0061] Figure 6 -(1) shows Figure 5 The analysis process is used for the process of deeply identifying the target phospholipid molecular structure in a single cell, and the deep structural identification of the phospholipid molecule includes the sn-position and C=C position of the fatty acid chain.

[0062] First, all target derivatized phospholipid ions are stored in the first linear ion trap 101. Then, ions of each mass-to-charge ratio are analyzed in turn; in a single cycle, derivatized phospholipid ions of a specific mass-to-charge ratio are analyzed by three transmissions. After the first transmission, MS is performed in the second linear ion trap 102. 3 After the second and third transmissions, MS is performed in the second linear ion trap 102. 4 Analysis is used to determine the C=C position on the fatty acid acyl chain at a specific sn-position.

[0063] Figure 6 -(2) and Figure 6 -(3) shows the MS analysis of the derivatized ion (mass-to-charge ratio, 903) of the phospholipid molecule PC 34:1. 3 and MS 4 Mass spectra and identification results. n Mass spectrum. Figure 6 -(2) It can be judged that PC 34:1 contains two fatty acid acyl chains, C16:0 and C18:1, and there are two isomers, PC 16:0 / 18:1 (mass-to-charge ratio, 396) and PC 18:1 / 16:0 (mass-to-charge ratio, 466). Figure 6 -(3) It can be judged that C18:1 located at the sn-2 and sn-1 positions includes two C=C positions, n-6 and n-9.

[0064] After a single injection, Figure 5 The described process can perform in-depth structural analysis of a variety of derivatized phospholipid ions, breaking through the current challenge of limited injection times for single-cell analysis.

[0065] Example 2: Figure 7 As shown, the embodiment of the present application takes the in-depth identification of the target phospholipid molecular structure in a single cell as an example, and introduces the phospholipid ions into the first linear ion trap of the dual linear ion trap mass spectrometer. After the injection, the ions enter the first linear ion trap 101 and are stored. Then, a cyclic analysis is performed until all the ions are analyzed. In a single cycle, the target mass-to-charge ratio range is first selected, and the first linear ion trap 101 transmits the ions to the second linear ion trap 102 according to the set number of transmissions and ratios. The simultaneous transmission of different ions within the mass-to-charge ratio range is achieved by adjusting the frequency of the first auxiliary signal 201 to vary with time; the transmission of different ratios of different ions within the mass-to-charge ratio range is achieved by adjusting the amplitude of the first auxiliary signal 201 to vary with time. The second linear ion trap 102 performs MS on all linear ion traps transmitted each time. 2 analyze.

[0066] Example 3: Figure 8 As shown in the figure, on the full mass-to-charge ratio axis, four mass-to-charge ratio ranges are set. The first mass-to-charge ratio range is set for 3 transmissions, the second mass-to-charge ratio range is set for 2 transmissions, the third mass-to-charge ratio range is set for 3 transmissions, and the fourth mass-to-charge ratio range is set for 2 transmissions. Fig. 9 The set mass-to-charge ratio range and the number of transmissions can finally obtain 10 MS 2 Spectrum. This analysis process ensures the ion utilization rate and improves the analysis speed, which is conducive to the analysis of trace samples. At the same time, sufficient mass spectrometry information can be obtained, which is conducive to subsequent spectrum analysis and can identify the structures of more molecules.

[0067] Next, the dual linear ion trap mass spectrometer proposed according to the embodiment of the present application is described with reference to the accompanying drawings.

[0068] Fig. 9 Schematic diagram of a dual linear ion trap mass spectrometer according to an embodiment of the present application.

[0069] like Fig. 9 As shown, the dual linear ion trap mass spectrometer 10 includes: a first linear ion trap 101 , a second linear ion trap 102 and a controller 106 .

[0070] Among them, the controller 106 is used to control the first linear ion trap 101 to transmit the target ions to the second linear ion trap 102 of the dual linear ion trap mass spectrometer in multiple times according to the first auxiliary signal, perform tandem mass spectrometry analysis on each transmitted ion according to the second auxiliary signal, and identify the complete ion structure of the sample to be tested based on the tandem mass spectrometry analysis data of each transmitted ion.

[0071] Specifically, if Fig.10 As shown, the dual linear ion trap device includes a first linear ion trap 101 , a second linear ion trap 102 , a first gate electrode 103 , a second gate electrode 104 , a third gate electrode 105 , a first auxiliary signal 201 , and a second auxiliary signal 202 .

[0072] like Fig.11 As shown, the first auxiliary signal 201 and the second auxiliary signal 202 are both sinusoidal signals, and the first linear ion trap 101 and the second linear ion trap 102 are driven by sinusoidal waves.

[0073] According to the dual linear ion trap mass spectrometer proposed in the embodiment of the present application, the first linear ion trap 101 is controlled according to the first auxiliary signal to transmit the target ions to the second linear ion trap 102 of the dual linear ion trap mass spectrometer in multiple times, and a tandem mass spectrometry analysis is performed on each transmitted ion according to the second auxiliary signal, and the complete ion structure of the sample to be tested is identified based on the tandem mass spectrometry analysis data of each transmitted ion; through the method of multiple analyses after a single injection, in-depth identification of multiple target ion structures can be achieved, and multiple MS analyses of multiple target ions can be performed without increasing the number of injections. n Analysis, thereby increasing the number of ions for complete structural identification; obtaining multiple MS images in a short time n Spectra accelerate the analysis process, enhance the ability to resolve structures, and have high applicability. They can be applied to the analysis of extremely trace samples to achieve in-depth identification of the structures of a variety of substances.

[0074] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or N embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0075] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, "N" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0076] Any process or method description in a flowchart or otherwise described herein may be understood to represent a module, fragment or portion of code comprising one or N executable instructions for implementing the steps of a custom logical function or process, and the scope of the preferred embodiments of the present application includes alternative implementations in which functions may not be performed in the order shown or discussed, including performing functions in a substantially simultaneous manner or in reverse order depending on the functions involved, which should be understood by technicians in the technical field to which the embodiments of the present application belong.

[0077] It should be understood that the various parts of the present application can be implemented by hardware, software, firmware or a combination thereof. In the above embodiment, the N steps or methods can be implemented by software or firmware stored in a memory and executed by a suitable instruction execution system. For example, if implemented by hardware, as in another embodiment, it can be implemented by any one or a combination of multiple of the following technologies known in the art: a discrete logic circuit having a logic gate circuit for implementing a logic function for a data signal, a dedicated integrated circuit having a suitable combination of logic gate circuits, a programmable gate array (PGA), a field programmable gate array (FPGA), etc.

[0078] A person skilled in the art may understand that all or part of the steps in the method for implementing the above-mentioned embodiment may be completed by instructing related hardware through a program, and the program may be stored in a computer-readable storage medium, which, when executed, includes one or a combination of the steps of the method embodiment.

Claims

1. An ion analysis method based on a dual linear ion trap mass spectrometer, characterized in that: The following steps are involved: Introducing ions of the sample to be tested into the first linear ion trap of the dual linear ion trap mass spectrometer; Selecting target ions in the first linear ion trap, wherein the first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, and the second linear ion trap performs tandem mass spectrometry analysis on each transmitted ion; The complete ion structure of the sample to be tested is identified based on the tandem mass spectrometry analysis data of each ion transmission.

2. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 1, characterized in that: The first linear ion trap controls the ratio and mass-to-charge ratio of ions transmitted each time based on the first auxiliary signal.

3. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 2, characterized in that: The first auxiliary signal controls the mass-to-charge ratio of ions transmitted by the first linear ion trap by adjusting the frequency.

4. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 3, characterized in that: The first auxiliary signal controls the ratio of ions transmitted by the first linear ion trap by adjusting the amplitude.

5. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 4, characterized in that: The first linear ion trap transmits the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times, including: Transferring ions of a target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer according to a target ratio; or, transmitting ions of target mass-to-charge ratio to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times according to target ratio, or, Simultaneously transmitting ions of different mass-to-charge ratios to the second linear ion trap of the dual linear ion trap mass spectrometer at different ratios, or, Ions with different mass-to-charge ratios are transmitted to the second linear ion trap of the dual linear ion trap mass spectrometer multiple times and simultaneously according to a target ratio.

6. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 5, characterized in that: The sum of the ratios of the multiple transmissions of ions of one mass-to-charge ratio does not exceed 100%, and the sum of the transmission ratios of each type of ions of different mass-to-charge ratios during the multiple transmissions does not exceed 100%.

7. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 1, characterized in that: The second linear ion trap realizes the excitation of ions based on the second auxiliary signal, so as to perform tandem mass spectrometry analysis on the ions transmitted each time.

8. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 7, characterized in that: The tandem mass spectrometry analysis includes MS 2 Analysis, MS 3 Analysis or MS 4 analyze.

9. The ion analysis method based on a dual linear ion trap mass spectrometer according to claim 2 or 7, characterized in that: The first auxiliary signal and the second auxiliary signal are sinusoidal waves or rectangular waves.

10. A dual linear ion trap mass spectrometer, characterized in that: include: a first linear ion trap and a second linear ion trap; The controller is used to control the first linear ion trap to transmit the target ions to the second linear ion trap of the dual linear ion trap mass spectrometer in multiple times according to the first auxiliary signal, perform tandem mass spectrometry analysis on each transmitted ion according to the second auxiliary signal, and identify the complete ion structure of the sample to be tested based on the tandem mass spectrometry analysis data of each transmitted ion.

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