Biological bacteriostatic agent with washing function

Abstract

The invention relates to a biological bacteriostatic agent with a washing function, and belongs to the technical field of daily chemical products, a preparation method of the biological bacteriostatic agent comprises the following steps: A1, mixing lysozyme and spirulina to obtain a lysozyme-spirulina compound; a2, adding chitosan, polyvinyl alcohol and tetrasodium glutamate diacetate into deionized water to obtain a mixed solution; adding the lysozyme-spirulina complex into the mixed solution, and stirring for 3-8 hours to obtain a composite antibacterial agent; and A3, mixing sapindus saponin, camellia saponin, the composite antibacterial agent, protease and essence to obtain the biological bacteriostatic agent with the washing function. Through the synergistic effect of all the components, the biological bacteriostatic agent has excellent decontamination, sterilization and bacteriostasis effects. The main raw materials used in the invention are green and environment-friendly, have no irritation, do not hurt the skin, have small influence on the environment, and have wide application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and in particular relates to a biological antibacterial agent with a washing function. Background Art

[0002] With the improvement of economic level, consumers pay more and more attention to healthy living, and have higher and higher requirements for sterilization and cleaning of clothing, toys, kitchen utensils, etc. At the same time, the cleaning work in public places such as restaurants, airports, hospitals, etc. also needs to meet the requirements of fast cleaning and efficient antibacterial.

[0003] Lysozyme is a specific hydrolase that can break the β-1,4 glycosidic bond between N-acetylglucosamine and N-acetylmuramic acid in the bacterial cell wall peptidoglycan. It can selectively dissolve the target microbial cell wall and inactivate it, achieving an antibacterial effect. It has high biocompatibility and has received more and more attention in recent years. However, the limitations of its antibacterial ability have also restricted its development. Therefore, the development of a biological antibacterial agent that can effectively sterilize and disinfect while washing and decontamination is of great significance and has broad market prospects. Summary of the invention

[0004] The object of the present invention is to provide a biological antibacterial agent with a washing function, wherein lysozyme is attached to the surface of spirulina, and then combined with chitosan through hydrogen bonds and electrostatic interactions, thereby significantly improving the stability of lysozyme, and then supplemented with tetrasodium glutamate diacetate and polyvinyl alcohol to synergistically improve the stability of lysozyme. The synergistic effect of spirulina, lysozyme and chitosan significantly improves the bactericidal and antibacterial effects. The raw materials used in the present invention are green and environmentally friendly, non-irritating, do not hurt the skin, have little impact on the environment, and have broad application prospects.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0007] A1. Add lysozyme into deionized water to obtain a lysozyme aqueous solution, add Spirulina into the lysozyme aqueous solution, stir evenly, incubate with shaking at 30-37° C. for 12-18 h, and centrifuge to obtain a lysozyme-Spirulina complex;

[0008] In the above preparation process, the positively charged lysozyme is combined with the negatively charged spirulina through electrostatic interaction, so that the lysozyme can be attached to the surface of the spirulina, thereby improving the stability of the lysozyme.

[0009] Furthermore, in step A1, the usage ratio of lysozyme, deionized water and spirulina is (0.03-0.06) g: (10-14) mL: (100-130) mL.

[0010] Furthermore, in step A1, the concentration of Spirulina is 0.5-1 mg / mL.

[0011] A2, adding chitosan, polyvinyl alcohol and tetrasodium glutamate diacetate into deionized water, stirring evenly to obtain a mixed solution; adding lysozyme-spirulina complex into the mixed solution, stirring for 3-8 hours, to obtain a composite antibacterial agent;

[0012] In the above preparation process, the lysozyme-spirulina complex is added to a mixed solution containing chitosan to prepare a composite antibacterial agent. The lysozyme-spirulina complex is combined with chitosan through hydrogen bonds and electrostatic interactions to improve the stability of the lysozyme. Tetrasodium glutamate diacetate and polyvinyl alcohol are then added to synergistically improve the stability of the lysozyme.

[0013] Furthermore, in step A2, the mass ratio of chitosan, polyvinyl alcohol, tetrasodium glutamate diacetate, deionized water and lysozyme-spirulina complex is (0.6-0.9):(1.1-1.3):(2.5-3.5):(70-90):(3.8-5.5).

[0014] A3. Mix and stir the soapberry saponin, camellia saponin, composite antibacterial agent, protease and essence to obtain a biological antibacterial agent with washing function.

[0015] In the above preparation process, sapindus saponin and camellia saponin are nonionic surfactants with excellent cleaning and decontamination performance. Protease can effectively degrade dirt such as fat and protein, and effectively remove microbial odor. Through the synergistic effect of sapindus saponin, camellia saponin and protease, excellent cleaning and decontamination performance is achieved.

[0016] Furthermore, in step A3, the mass ratio of soapberry saponin, camellia saponin, composite antibacterial agent, protease and essence is (3-10): (1-4): (80-100): (0.5-1.2): (0.1-0.3).

[0017] Beneficial effects of the present invention:

[0018] (1) In the technical solution of the present invention, lysozyme is attached to the surface of Spirulina and then combined with chitosan through hydrogen bonds and electrostatic interactions, which significantly improves the stability of lysozyme. Tetrasodium glutamate diacetate and polyvinyl alcohol are then added to synergistically improve the stability of lysozyme.

[0019] (2) In the technical solution of the present invention, Spirulina has a spiral structure, which is easy to combine with the biofilm and can bring lysozyme to the surface of the biofilm. Lysozyme is a specific hydrolase that can break the β-1,4 glycosidic bond between N-acetylglucosamine and N-acetylmuramic acid in the bacterial cell wall peptidoglycan, which can dissolve the target microbial cell wall and inactivate it, making it easier for chitosan to penetrate into the cell membrane. Chitosan can chelate with lipopolysaccharide on the surface of the cell membrane, making the cell membrane easier to be destroyed. Through the synergistic effect of Spirulina, lysozyme and chitosan, the bactericidal and antibacterial effect is significantly improved. In addition, Spirulina releases active oxygen under the stimulation of light, which can destroy the integrity of the biofilm, making it easier for lysozyme to enter the depth of the biofilm and act on the cell wall, thereby improving the bactericidal and antibacterial effect.

[0020] (3) In the technical solution of the present invention, sapindus saponin and camellia saponin are nonionic surfactants with excellent cleaning and decontamination performance. Protease can effectively degrade dirt such as fat and protein, and effectively remove microbial odors. Through the synergistic effect of sapindus saponin, camellia saponin and protease, excellent cleaning and decontamination performance is achieved.

[0021] (4) In the technical solution of the present invention, the synergistic effect between the components makes the prepared biological antibacterial preparation have excellent decontamination and bactericidal and antibacterial effects, and can also remove odor. The main raw materials used in the present invention are green and environmentally friendly, non-irritating, and do not hurt the skin, have little impact on the environment, and have broad application prospects. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0025] A1. Add 6 g of lysozyme to 2 L of deionized water to obtain a lysozyme aqueous solution, add 20 L of 0.5 mg / mL spirulina to the lysozyme aqueous solution, stir evenly, incubate with shaking at 30° C. for 12 h, and centrifuge to obtain a lysozyme-spirulina complex;

[0026] A2, adding 1.2g chitosan, 2.2g polyvinyl alcohol and 5g tetrasodium glutamate diacetate into 140g deionized water, stirring evenly to obtain a mixed solution; adding 7.6g lysozyme-spirulina complex into the mixed solution, stirring for 3h, to obtain a composite antibacterial agent;

[0027] A3. Mix and stir 3 g of soapberry saponin, 1 g of camellia saponin, 80 g of the composite antibacterial agent, 0.5 g of protease and 0.1 g of lavender essence to obtain a biological antibacterial agent with a washing function.

[0028] Example 2

[0029] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0030] A1. Add 8 g of lysozyme to 2.1 L of deionized water to obtain a lysozyme aqueous solution, add 22 L of 0.6 mg / mL spirulina to the lysozyme aqueous solution, stir evenly, incubate at 32° C. with shaking for 13 h, and centrifuge to obtain a lysozyme-spirulina complex;

[0031] A2, 1.4g chitosan, 2.3g polyvinyl alcohol and 5.4g tetrasodium glutamate diacetate were added to 150g deionized water, and stirred evenly to obtain a mixed solution; 8g lysozyme-spirulina complex was added to the mixed solution, and stirred for 4h to obtain a composite antibacterial agent;

[0032] A3. Mix and stir 4 g of soapberry saponin, 2 g of camellia saponin, 85 g of composite antibacterial agent, 0.6 g of protease and 0.12 g of lavender essence to obtain a biological antibacterial agent with washing function.

[0033] Example 3

[0034] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0035] A1. Add 9 g of lysozyme to 2.4 L of deionized water to obtain a lysozyme aqueous solution, add 23 L of 0.7 mg / mL spirulina to the lysozyme aqueous solution, stir evenly, incubate at 34°C with shaking for 15 h, and centrifuge to obtain a lysozyme-spirulina complex;

[0036] A2, 1.7g chitosan, 2.4g polyvinyl alcohol and 6g tetrasodium glutamate diacetate were added to 160g deionized water, and stirred evenly to obtain a mixed solution; 9.5g lysozyme-spirulina complex was added to the mixed solution, and stirred for 5h to obtain a composite antibacterial agent;

[0037] A3. Mix and stir 6 g of soapberry saponin, 2.3 g of camellia saponin, 90 g of the composite antibacterial agent, 0.8 g of protease and 0.2 g of lavender essence to obtain a biological antibacterial agent with a washing function.

[0038] Example 4

[0039] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0040] A1. Add 10 g of lysozyme to 2.7 L of deionized water to obtain a lysozyme aqueous solution, add 24 L of 0.8 mg / mL spirulina to the lysozyme aqueous solution and stir evenly, incubate at 37°C with shaking for 17 h, and centrifuge to obtain a lysozyme-spirulina complex;

[0041] A2, 1.7g chitosan, 2.5g polyvinyl alcohol and 6.7g tetrasodium glutamate diacetate were added to 170g deionized water, and stirred evenly to obtain a mixed solution; 10.3g lysozyme-spirulina complex was added to the mixed solution, and stirred for 6h to obtain a composite antibacterial agent;

[0042] A3. Mix and stir 8 g of soapberry saponin, 3 g of camellia saponin, 95 g of composite antibacterial agent, 1 g of protease and 0.25 g of lavender essence to obtain a biological antibacterial agent with washing function.

[0043] Example 5

[0044] A biological antibacterial agent with a washing function, the preparation method comprising the following steps:

[0045] A1. Add 12 g of lysozyme to 2.8 L of deionized water to obtain a lysozyme aqueous solution, add 26 L of 1 mg / mL spirulina to the lysozyme aqueous solution and stir evenly, incubate with shaking at 37°C for 18 h, and centrifuge to obtain a lysozyme-spirulina complex;

[0046] A2, 1.8g chitosan, 2.6g polyvinyl alcohol and 7g tetrasodium glutamate diacetate were added to 180g deionized water, and stirred evenly to obtain a mixed solution; 11g lysozyme-spirulina complex was added to the mixed solution, and stirred for 8h to obtain a composite antibacterial agent;

[0047] A3. Mix and evenly stir 10 g of soapberry saponin, 4 g of camellia saponin, 100 g of the composite antibacterial agent, 1.2 g of protease and 0.3 g of lavender essence to obtain a biological antibacterial agent with a washing function.

[0048] Comparative Example 1

[0049] Compared with Example 3, the operation of step A2 was not performed in Comparative Example 1, and the other steps and raw materials were the same as those in Example 3.

[0050] Comparative Example 2

[0051] Compared with Example 3, the operation of step A1 was not performed in Comparative Example 2, and the other steps and raw materials were the same as those in Example 3.

[0052] Comparative Example 3

[0053] Compared with Example 3, steps A1 and A2 were not performed in Comparative Example 3, and other steps and raw materials were the same as those in Example 3.

[0054] Performance Testing

[0055] (1) Antibacterial performance test: The antibacterial performance test was carried out according to QB / T 2738-2023 Evaluation method for antibacterial and antibacterial effects of daily chemical products. The results are shown in Table 1.

[0056] (2) Stability test: After the samples were placed for 12 months, the antibacterial ability test was performed again. The results are shown in Table 2;

[0057] (3) Detergent performance test: The detergent performance test was carried out in accordance with GB / T 13174-2021 Detergent power and cyclic washing performance of detergents for clothing. The results are shown in Table 3.

[0058] Table 1

[0059]

[0060]

[0061] Table 2

[0062]

[0063] Table 3

[0064]

[0065] It can be seen from the data in Table 1, Table 2 and Table 3 that the biological antibacterial preparation prepared by the present invention has excellent antibacterial performance, good stability, and excellent decontamination performance. It can be seen from the data of Comparative Example 3 and Comparative Example 1 that the antibacterial performance and stability of the biological antibacterial preparation are reduced due to the fact that chitosan, polyvinyl alcohol and tetrasodium glutamate diacetate are not added during the preparation process. It can be seen from the data of Comparative Example 3 and Comparative Example 2 that the antibacterial performance and stability of the biological antibacterial preparation are reduced due to the fact that spirulina is not added. It can be seen from the data of Comparative Example 3 and Comparative Example 3 that the antibacterial performance and stability are poor when only lysozyme is added alone, indicating that the addition of spirulina, polysaccharide, polyvinyl alcohol and tetrasodium glutamate diacetate can significantly improve the stability and antibacterial performance of lysozyme.

[0066] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0067] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A biological antibacterial agent with washing function, characterized in that: The preparation method comprises the following steps: A1. Add lysozyme into deionized water to obtain a lysozyme aqueous solution, add Spirulina into the lysozyme aqueous solution, stir evenly, incubate with shaking, and centrifuge to obtain a lysozyme-Spirulina complex; A2, adding chitosan, polyvinyl alcohol and tetrasodium glutamate diacetate into deionized water, stirring evenly to obtain a mixed solution; adding lysozyme-spirulina complex into the mixed solution, stirring for a period of time, and obtaining a composite antibacterial agent; A3. Mix and stir the soapberry saponin, camellia saponin, composite antibacterial agent, protease and essence to obtain a biological antibacterial agent with washing function.

2. The biological antibacterial agent with washing function according to claim 1, characterized in that: In the step A1, the temperature during the shaking incubation is 30-37° C. and the time is 12-18 hours.

3. The biological antibacterial agent with washing function according to claim 1, characterized in that: In the step A1, the usage ratio of lysozyme, deionized water and spirulina is (0.03-0.06) g: (10-14) mL: (100-130) mL.

4. The biological antibacterial agent with washing function according to claim 1, characterized in that: In the step A1, the concentration of Spirulina is 0.5-1 mg / mL.

5. The biological antibacterial agent with washing function according to claim 1, characterized in that: In step A2, the stirring time is 3-8 hours.

6. The biological antibacterial agent with washing function according to claim 1, characterized in that: In the step A2, the mass ratio of chitosan, polyvinyl alcohol, tetrasodium glutamate diacetate, deionized water and lysozyme-spirulina complex is (0.6-0.9): (1.1-1.3): (2.5-3.5): (70-90): (3.8-5.5).

7. The biological antibacterial agent with washing function according to claim 1, characterized in that: In the step A3, the mass ratio of soapberry saponin, camellia saponin, composite antibacterial agent, protease and essence is (3-10): (1-4): (80-100): (0.5-1.2): (0.1-0.3).

8. The biological antibacterial agent with washing function according to claim 7, characterized in that: In the step A3, the mass ratio of soapberry saponin, camellia saponin, composite antibacterial agent, protease and essence is (4-7): (2-3): (90-100): (0.6-1): (0.1-0.2).

9. The biological antibacterial agent with washing function according to claim 1, characterized in that: In step A3, the essence includes at least one of lavender essence, rose essence, jasmine essence, magnolia essence, and clove essence.

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