Fructus citri sarcodactylis compound protein powder as well as preparation method and application thereof

Abstract

The invention provides fingered citron compound protein powder as well as a preparation method and application thereof, and relates to the technical field of functional foods. The fingered citron compound protein powder comprises fingered citron extract microcapsules, soybean protein isolate powder, polydextrose, resistant dextrin, soybean peptide powder, tremella powder and the like as main components, and a nutrient supplement and other auxiliary raw materials are added. The fingered citron extract disclosed by the invention is prepared through supercritical CO2 extraction and microencapsulation technologies, so that the stability and effectiveness of components are ensured, and the solubility and taste of the fingered citron extract are improved at the same time. The preparation process provided by the invention is simple and efficient, the traditional Chinese medicinal material fingered citron can be combined with a modern nutrition process, and the effects of soothing liver and regulating qi of fingered citron are utilized to enhance the weight loss effect and maintain muscle quality. The product disclosed by the invention is excellent in nutritional value, functionality and safety, can be widely applied to the field of healthy foods, and is used for reducing weight and relieving depression and anxiety symptoms.

Description

Technical Field

[0001] The invention relates to the technical field of functional foods, and in particular to a bergamot composite protein powder and a preparation method and application thereof. Background Art

[0002] Obesity refers to a certain degree of obvious overweight and excessive fat layer, which is a state caused by excessive accumulation of body fat, especially triglycerides. At present, obesity has become a global health problem and a high-risk factor for many chronic diseases such as cardiovascular disease, diabetes, cancer, etc., and obese patients are often accompanied by anxiety and depression.

[0003] The main causes of obesity include imbalance between energy intake and consumption, genetics and metabolism, unhealthy lifestyle, etc. At present, although common methods such as dieting can achieve weight loss, they generally lead to a decrease in muscle mass.

[0004] As a traditional Chinese medicine, bergamot has a significant effect on soothing the liver and regulating qi. Combining it with protein powder is expected to reduce weight while avoiding the loss of muscle mass. However, combining bergamot ingredients with protein powder to prepare a compound nutritional product faces many technical challenges, such as the extraction and stability of bergamot ingredients, compatibility with protein powder, and product taste adjustment. Summary of the invention

[0005] In view of this, the present invention provides a bergamot composite protein powder, a preparation method and application thereof. The present invention is prepared by supercritical CO2 extraction and microencapsulation technology, which ensures the stability and effectiveness of the components of the bergamot extract, and also improves the solubility and taste of the product. The preparation process of the bergamot composite protein powder of the present invention is simple and efficient, combining the traditional Chinese medicine bergamot with modern nutritional technology, using the liver-soothing and qi-regulating effects of bergamot, enhancing the weight loss effect and maintaining muscle quality. The product is excellent in nutritional value, functionality and safety, and can be widely used in the field of health foods for weight loss and relief of depression and anxiety symptoms, and has good application prospects.

[0006] The bergamot composite protein powder of the present invention comprises, by weight:

[0007] 6-10 parts of bergamot extract microcapsules, 45-50 parts of isolated soy protein powder, 10-20 parts of polydextrose, 8-10 parts of resistant dextrin, 6-10 parts of soy peptide powder, 5-7 parts of tremella powder, 1-2 parts of edible salt, 0-2 parts of edible flavor, 1-3 parts of nutritional enhancer, 0.05-0.1 parts of sweetener and 1-2 parts of anti-caking agent.

[0008] The preferred bergamot composite protein powder comprises, by weight:

[0009] 8 parts of bergamot extract microcapsules, 48 ​​parts of isolated soy protein powder, 15 parts of polydextrose, 9 parts of resistant dextrin, 8.58 parts of soy peptide powder, 6.07 parts of tremella powder, 1.6 parts of edible salt, 1.5 parts of edible flavors, 2.12 parts of nutritional enhancers, 0.08 parts of sweeteners, and 1.5 parts of anticaking agents.

[0010] Preferably, the nutritional enhancer is at least one of concentrated milk protein powder, calcium caseinate, and linseed oil microcapsule powder. More preferably, the nutritional enhancer comprises, by weight: 0.66 parts of concentrated milk protein powder, 0.66 parts of calcium caseinate, and 0.8 parts of linseed oil microcapsule powder.

[0011] Preferably, the preparation method of the bergamot extract microcapsules comprises the following steps:

[0012] S1. Preparation of bergamot extract: pre-treating bergamot and performing supercritical CO2 extraction, and drying the extract to obtain bergamot extract;

[0013] S2. Microencapsulation of bergamot extract: dissolve sodium alginate in deionized water to obtain solution A, dissolve chitosan in acetic acid solution to obtain solution B, mix bergamot extract, emulsifier and water evenly to obtain solution C, then slowly drop solution C into solution A to obtain emulsion, then slowly drop the emulsion into solution B, filter, wash and dry after the reaction is completed to obtain bergamot extract microcapsules.

[0014] Preferably, the extraction pressure of the supercritical CO2 extraction is 20-50 MPa, the extraction temperature is 35°C-60°C, and the extraction time is 1-4 hours.

[0015] Preferably, in the solution A, the concentration of sodium alginate is 2 wt.% to 5 wt.%.

[0016] Preferably, in the solution B, the concentration of chitosan is 1 wt.% to 3 wt.%.

[0017] Preferably, in the solution C, the concentration of the bergamot extract is 10 wt.% to 30 wt.%, and the concentration of the emulsifier is 0.5 wt.% to 2 wt.%.

[0018] Preferably, the mass ratio of the bergamot extract to the sodium alginate and chitosan is 80:1:1.

[0019] The present invention provides a method for preparing bergamot composite protein powder, comprising the following steps:

[0020] The raw materials are weighed in proportion, and then the soy protein isolate powder and resistant dextrin are premixed by low-speed shearing, followed by adding bergamot extract microcapsules and polydextrose for a high-speed shearing mixing, and finally the remaining raw materials are added for a second high-speed shearing mixing. After all the raw materials are evenly mixed, bergamot composite protein powder is obtained.

[0021] The present invention also provides an application of a bergamot compound protein powder in reducing weight and alleviating depression and anxiety symptoms. The bergamot compound protein powder is the bergamot compound protein powder described in the above technical solution.

[0022] Compared with the prior art, the present invention adopts a series of innovative processes to ensure the stability and effectiveness of the bergamot ingredients, while improving the solubility and overall taste of the protein powder, and overcoming the technical difficulties of combining the bergamot ingredients with the protein powder. On the basis of the traditional protein powder preparation process, the present invention combines the medicine and food homologous material bergamot with modern nutrition and technology in combination with the theory of Chinese medicine health preservation, and through the liver-soothing and qi-regulating effects of bergamot, a synergistic effect is played on the raw materials of protein, so that the product of the present invention can help maintain muscle mass while enhancing the weight loss effect, and meet the multiple needs of obese patients and healthy people. The present invention optimizes the ratio and mixing order of each raw material, adopts a mixing method combined with low-speed shear premixing and high-speed shearing, ensures that each component is evenly dispersed, and at the same time improves the solubility and suspension of the product through the use of a variety of additives. The bergamot composite protein powder of the present invention has obvious advantages in stability, nutritional value, taste, functionality and safety, and is a healthy food with broad application prospects. DETAILED DESCRIPTION

[0023] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The present invention provides a bergamot composite protein powder, which comprises, by weight:

[0025] 6-10 parts of bergamot extract microcapsules, 45-50 parts of isolated soy protein powder, 10-20 parts of polydextrose, 8-10 parts of resistant dextrin, 6-10 parts of soy peptide powder, 5-7 parts of tremella powder, 1-2 parts of edible salt, 1-2 parts of edible flavor, 1-3 parts of nutritional enhancer, 0.05-0.1 parts of sweetener and 1-2 parts of anti-caking agent.

[0026] In some preferred embodiments of the present invention, the bergamot composite protein powder comprises, by weight:

[0027] 8 parts of bergamot extract microcapsules, 48 ​​parts of isolated soy protein powder, 15 parts of polydextrose, 9 parts of resistant dextrin, 8.58 parts of soy peptide powder, 6.07 parts of tremella powder, 1.6 parts of edible salt, 1.5 parts of edible flavors, 2.12 parts of nutritional enhancers, 0.08 parts of sweeteners, and 1.5 parts of anticaking agents.

[0028] In some specific embodiments of the present invention, the nutritional enhancer is preferably at least one of concentrated milk protein powder, calcium caseinate, and linseed oil microcapsule powder.

[0029] Concentrated milk protein powder is a powder with high protein content obtained by extracting protein from milk by physical method, removing part of lactose and fat. In the present invention, concentrated milk protein powder provides high-quality protein, which is helpful for muscle repair and growth.

[0030] Calcium caseinate is prepared by acid treatment and calcium salting of casein. In the present invention, calcium caseinate can provide high-quality protein and calcium, which is helpful for bone health and muscle repair. At the same time, calcium caseinate has good solubility and stability, and can remain stable in an acidic environment, which is of great significance to improving the stability of the protein powder of the present invention.

[0031] The linseed oil microcapsule powder is prepared from linseed oil by microencapsulation technology. In the present invention, the linseed oil microcapsule powder can provide Omega-3 fatty acids, which are helpful for cardiovascular health, anti-inflammatory and immune support. At the same time, the microencapsulation technology can make the effective ingredients slowly released and absorbed, improve the utilization rate of nutrition, and protect the activity of the product to the maximum extent, without being affected by other components, prolong the shelf life and improve the stability.

[0032] In some preferred embodiments of the present invention, the nutritional enhancer comprises, by weight: 0.66 parts of concentrated milk protein powder, 0.66 parts of calcium caseinate, and 0.8 parts of linseed oil microcapsule powder.

[0033] The present invention uses these ingredients as nutritional enhancers together, and can utilize the synergistic effect of the three raw materials to bring out better nutritional and health benefits. First, concentrated milk protein powder and calcium caseinate are both rich in high-quality protein, which contains essential amino acids, can promote muscle growth and repair, and improve the body's absorption and utilization rate of protein. The different combinations of these two protein sources help to provide a more comprehensive amino acid spectrum to meet the human body's needs for different amino acids. In addition, calcium caseinate is not only a high-quality protein source, but also a good calcium source. It can effectively enhance bone density and prevent osteoporosis. At the same time, the calcium in calcium caseinate is more easily absorbed and utilized by the human body, especially in the case of sufficient vitamin D, this effect is more obvious. The flaxseed oil microcapsule powder is rich in α-linolenic acid. By making flaxseed oil into a microcapsule powder, not only its stability can be improved, but also its application range in food can be increased, so that these health benefits can be better exerted.

[0034] The three ingredients used together can not only provide comprehensive nutritional support, but also play a positive role in food processing, such as improving the stability and solubility of compound protein powder and extending the shelf life, making it an ideal compound nutritional enhancer.

[0035] In a specific embodiment of the present invention, the sweetener is at least one of mogroside, rubusoside, steviol glycoside, erythritol, isomalt, and xylitol. The sweetener used in the present invention is not only low in calories and has little effect on blood sugar, but also comes from natural plants and has high safety, and is suitable for use by diabetic patients, people who want to lose weight, and people who are sensitive to traditional sugar.

[0036] In some specific embodiments of the present invention, the anti-caking agent is at least one of potassium ferrocyanide, tricalcium phosphate, silicon dioxide, and microcrystalline cellulose.

[0037] Anticaking agents, also known as anti-caking agents, are food additives used to prevent granular or powdered foods from agglomerating and caking, and to keep them loose or flowing. The present invention utilizes the characteristics of fine, loose and porous anticaking agent particles, strong adsorption, and easy adsorption to keep the composite protein powder in a powder state, thereby improving the stability of the product.

[0038] The preparation method of the bergamot extract microcapsules of the present invention comprises the following steps:

[0039] S1. Preparation of bergamot extract: pre-treating bergamot and performing supercritical CO2 extraction, and drying the extract to obtain bergamot extract;

[0040] S2. Microencapsulation of bergamot extract: dissolve sodium alginate in deionized water to obtain solution A, dissolve chitosan in acetic acid solution to obtain solution B, mix bergamot extract, emulsifier and water evenly to obtain solution C, then slowly drop solution C into solution A to obtain emulsion, then slowly drop the emulsion into solution B, filter, wash and dry after the reaction is completed to obtain bergamot extract microcapsules.

[0041] In some specific embodiments of the present invention, before pre-treating the bergamot, the raw materials are firstly screened, and fresh or dry bergamots without pests and diseases and with appropriate maturity are selected as raw materials.

[0042] In some specific embodiments of the present invention, the pretreatment is washing, slicing or washing and crushing. The present invention does not strictly limit the specific standards for slicing and crushing of bergamot, and can be flexibly adjusted according to actual needs and processing conditions. The purpose of doing so is to ensure that the bergamot can achieve the best extraction effect in the subsequent processing process, and to ensure the maximum retention of its nutrients and active substances.

[0043] In some specific embodiments of the present invention, the extraction pressure of the supercritical CO2 extraction is 20-50 MPa, the extraction temperature is 35°C-60°C, and the extraction time is 1-4 hours. Under this condition, the CO2 fluid can effectively dissolve and extract the active ingredients in the bergamot.

[0044] The reason why the present invention adopts supercritical CO2 extraction technology to prepare the bergamot extract is that the method can efficiently extract the active ingredients such as volatile oil, flavonoids, alkaloids, etc. in the bergamot under relatively mild conditions. And the method does not have the problem of solvent residue, ensuring the purity and safety of the extract, which is particularly important in the food field. At the same time, the present invention accurately controls the solubility of supercritical CO2 by controlling the temperature and pressure during the extraction process, thereby selectively extracting the target components required by the present invention, thereby obtaining a high-purity target product and reducing unnecessary impurities.

[0045] In some specific embodiments of the present invention, in the solution A, the concentration of sodium alginate is preferably 2 wt.% to 5 wt.%.

[0046] In some specific embodiments of the present invention, in the solution B, the concentration of chitosan is preferably 1 wt.% to 3 wt.%.

[0047] In some specific embodiments of the present invention, in the solution C, the concentration of the bergamot extract is preferably 10wt.% to 30wt.%, and the concentration of the emulsifier is preferably 0.5wt.% to 2wt.%.

[0048] In some specific embodiments of the present invention, the emulsifier is Tween 80.

[0049] In some specific embodiments of the present invention, the mass ratio of the bergamot extract to the sodium alginate and chitosan is 80:1:1.

[0050] In some preferred embodiments of the present invention, the solidification of the wall material and the formation of the microcapsules can be accelerated by adjusting the pH value or adjusting the reaction temperature.

[0051] Sodium alginate has good film-forming properties. During the microencapsulation process, it can form a stable embedding layer to encapsulate the bergamot extract and form a microcapsule structure. After sodium alginate is cross-linked with calcium ions or other divalent cations, it can form a gel network, further enhancing the stability and mechanical strength of the microcapsules. This cross-linking effect helps protect the active ingredients inside and prevent them from leaking or degrading during storage and transportation.

[0052] Chitosan has good biocompatibility and biodegradability. In the process of microencapsulation, chitosan is used as a wall material, and the microcapsule structure formed with sodium alginate is further enhanced, improving the stability and protective effect of the microcapsule. Chitosan has a certain sustained release performance, which can control the release rate of bergamot extract, prolong its action time in the body, and improve the efficacy and user experience of the product.

[0053] Emulsifiers can reduce the surface tension between the water phase and the oil phase, allowing the bergamot extract to mix better with the water phase to form a stable emulsion, which is crucial to ensure that the bergamot extract is evenly distributed throughout the system. Emulsifiers can also act as dispersants to prevent the bergamot extract from aggregating or precipitating during the preparation process, ensuring the uniformity and stability of the final microcapsules.

[0054] The present invention utilizes the synergistic effect of sodium alginate, chitosan and emulsifier to make the bergamot extract microcapsule not only have good stability and protective effect, but also can slowly release active ingredients under specific conditions (in vivo), thereby improving its application effect.

[0055] The present invention provides a method for preparing bergamot composite protein powder, comprising the following steps:

[0056] The raw materials are weighed in proportion, and then the soy protein isolate powder and resistant dextrin are premixed by low-speed shearing, followed by adding bergamot extract microcapsules and polydextrose for a high-speed shearing mixing, and finally the remaining raw materials are added for a second high-speed shearing mixing. After all the raw materials are evenly mixed, bergamot composite protein powder is obtained.

[0057] In some specific embodiments of the present invention, the low-speed shear premixing and high-speed shear mixing are carried out in a high-speed shear mixer. In some preferred embodiments of the present invention, the shear rate of the low-speed shear premixing is 100-450 rpm, the mixing time is 5-15 min, and the mixing temperature is 20°C-40°C; the shear rate of the first high-speed shear mixing is 500-2000 rpm, the mixing time is 3-10 min, and the mixing temperature is 25°C-45°C; the shear rate of the second high-speed shear mixing is 1000-3000 rpm, the mixing time is 8-15 min, and the mixing temperature is 30°C-50°C.

[0058] The present invention optimizes the ratio and mixing order of each raw material, adopts high-speed shear mixing combined with low-speed shear premixing to ensure uniform dispersion of each component, and optimizes the texture and taste of the product. The present invention does not strictly limit the shear rate, mixing time and mixing temperature of high-speed shearing and low-speed premixing, and can be adjusted according to actual conditions to achieve the best mixing effect.

[0059] The present invention also provides an application of a bergamot compound protein powder in reducing weight and alleviating depression and anxiety symptoms. The bergamot compound protein powder is the bergamot compound protein powder described in the above technical solution.

[0060] In order to further illustrate the present invention, the following examples are provided for detailed description. The raw materials used in the following examples of the present invention are all commercially available.

[0061] Unless otherwise specified, all experiments were repeated three times. SPSS 21.0 was used for analysis of variance (ANOVA) and Duncan's multiple comparison analysis. The results were expressed as mean ± standard deviation, and P < 0.05 indicated a significant difference.

[0062] Embodiment 1 A method for preparing a bergamot composite protein powder, comprising the following specific steps:

[0063] Weigh the raw materials in proportion, and then premix the soy protein isolate powder and the resistant dextrin at 30°C for 10 minutes at a shear rate of 300 rpm under low-speed shearing, then add the bergamot extract microcapsules and polydextrose, and mix them at a shear rate of 1000 rpm at 30°C for 8 minutes under high-speed shearing, and finally add the remaining raw materials, and mix them at a shear rate of 2000 rpm at 40°C for 12 minutes under high-speed shearing, and after all the raw materials are evenly mixed, obtain the bergamot composite protein powder;

[0064] The raw materials are specifically as follows in parts by mass:

[0065] 8 parts of bergamot extract microcapsules, 48 ​​parts of isolated soy protein powder, 15 parts of polydextrose, 9 parts of resistant dextrin, 8.58 parts of soy peptide powder, 6.07 parts of tremella powder, 1.6 parts of edible salt, 1.5 parts of edible flavor, 2.12 parts of nutritional enhancer, 0.08 parts of steviol glycoside, and 1.5 parts of silicon dioxide;

[0066] The nutritional enhancer is specifically composed of 0.66 parts of concentrated milk protein powder, 0.66 parts of calcium caseinate, and 0.8 parts of linseed oil microcapsule powder in parts by mass.

[0067] The preparation method of the bergamot extract microcapsule is as follows:

[0068] S1. Preparation of bergamot extract: fresh bergamot without pests and diseases and of suitable maturity is selected as raw material, after washing and slicing, the extraction pressure is set to 40MPa, the extraction temperature is set to 45°C, and the extraction time is set to 2.5h for supercritical CO2 extraction, and the extract is dried to obtain bergamot extract;

[0069] S2. Microencapsulation of bergamot extract: sodium alginate is dissolved in deionized water to obtain solution A with a sodium alginate concentration of 3.5 wt.%, chitosan is dissolved in acetic acid solution to obtain solution B with a chitosan concentration of 2 wt.%, bergamot extract, Tween 80 and water are evenly mixed to obtain solution C with a bergamot extract concentration of 20 wt.% and a Tween 80 concentration of 1 wt.%, then solution C is slowly dropped into solution A to obtain an emulsion, and then the emulsion is slowly dropped into solution B, wherein the mass ratio of the bergamot extract to the sodium alginate and chitosan is 80:1:1, and after the reaction is completed, the mixture is filtered, washed, and dried at 45°C for 60 min to obtain bergamot extract microcapsules.

[0070] Comparative Example 1

[0071] Same as Example 1, except that: no bergamot extract was added.

[0072] Comparative Example 2

[0073] Same as Example 1, except that an equal amount of bergamot extract is used instead of bergamot extract microcapsules.

[0074] Test Example 1 Microcapsule Performance Test

[0075] (1) Embedding rate:

[0076] Detection method: High performance liquid chromatography (HPLC) was used to determine the changes in the content of flavonoids in bergamot extract before and after microencapsulation and to calculate the embedding rate.

[0077] The results showed that the embedding rate of the bergamot extract microcapsules prepared in Example 1 was 85%, indicating that the preparation of the bergamot extract microcapsules of the present invention was able to successfully embed most of the active ingredients inside the microcapsules.

[0078] (2) Moisture content:

[0079] Detection method: Karl Fischer titration was used to determine the moisture content in the microcapsules.

[0080] The results showed that the moisture content of the bergamot extract microcapsules prepared in Example 1 was 3.5%, which can ensure that the microcapsules will not deteriorate due to excessive moisture during storage.

[0081] (3) Storage stability:

[0082] Detection method: The bergamot extract microgel prepared in Example 1 was stored at 25° C., 4° C. and −18° C. for 3 months and 6 months, respectively, and the content of active ingredients was detected regularly and the color and smell of the product were observed.

[0083] The results showed that the active ingredient loss rate of the bergamot extract microcapsules prepared in Example 1 was 7.8% after being stored at room temperature of 25°C for 6 months, and the active ingredient loss rates were 5% and 2% after being stored at 4°C and -18°C for 6 months, respectively. The color and smell of the bergamot extract microcapsules did not change significantly during the storage process, indicating that the bergamot extract microcapsules of the present invention have good storage stability.

[0084] (4) Particle size distribution:

[0085] Detection method: The particle size distribution of microcapsules was determined using a laser particle size analyzer.

[0086] The results showed that the particle size of the bergamot extract microcapsules prepared in Example 1 was mainly distributed between 10 and 50 μm, and the particle size distribution was uniform, which was conducive to the dissolution and dispersion of the product.

[0087] (5) Solubility and suspension:

[0088] Detection method: Add 1.0 g of microcapsules into 100 mL of deionized water, stir until completely dispersed, and observe its solubility and suspension.

[0089] The results showed that the bergamot extract microcapsules prepared in Example 1 had good solubility and suspension properties, and could be quickly dispersed in water to form a uniform suspension.

[0090] Test Example 2 Clinical Effect and Safety Testing

[0091] 1. Subject inclusion and grouping

[0092] 1. Inclusion criteria:

[0093] ① Overweight or obese adult patients aged 18-65 years (BMI ≥ 25 kg / m 2 );

[0094] ② With varying degrees of depressive symptoms, confirmed by psychological assessment;

[0095] ③ No serious heart, liver, kidney or other organ diseases, no cognitive impairment, etc.;

[0096] ④No history of allergy to bergamot or high-efficiency protein powder.

[0097] 2. Grouping: The included subjects were randomly divided into an experimental group and a control group, with 100 cases in each group. All subjects signed informed consent and agreed to accept a 12-week intervention.

[0098] 2. Dosage regimen

[0099] 1. Experimental Group 1:

[0100] ① Take the Buddha's hand composite protein powder of Example 1 daily, specifically 10 g each time, twice a day, within 30 minutes after breakfast and dinner respectively.

[0101] ②The packaging of the products is carefully designed to ensure the subjects' acceptance and compliance. At the same time, the subjects receive dietary guidance and exercise advice from nutritionists to maintain a healthy lifestyle.

[0102] 2. Control group:

[0103] ① Do not take any weight loss products, only accept diet guidance and exercise advice from nutritionists. And keep consistent with the experimental group to exclude the influence of non-product factors.

[0104] 3. Experimental Group 2

[0105] ① Take the protein powder of Comparative Example 1 daily, specifically 10 g each time, twice a day, within 30 minutes after breakfast and dinner respectively.

[0106] ② The packaging of the product is the same as the experimental group to ensure the subjects' acceptance and compliance. At the same time, the subjects receive dietary guidance and exercise advice from nutritionists to maintain a healthy lifestyle.

[0107] 4. Experimental Group 3

[0108] ① Take the protein powder of Comparative Example 2 daily, specifically 10 g each time, twice a day, within 30 minutes after breakfast and dinner respectively.

[0109] ② The packaging of the product is the same as the experimental group to ensure the subjects' acceptance and compliance. At the same time, the subjects receive dietary guidance and exercise advice from nutritionists to maintain a healthy lifestyle.

[0110] Effect evaluation:

[0111] To ensure that the effect is caused by the product and not by dietary guidance and exercise advice, we will take the following measures:

[0112] All subjects were randomized to ensure that the baseline characteristics of each group were consistent.

[0113] The subjects' physiological indicators such as weight, body fat percentage, blood pressure, blood sugar, and psychological status assessment (such as depression and anxiety symptoms) before and after the experiment were collected and analyzed.

[0114] A double-blind experimental design was used, meaning that neither the subjects nor the researchers knew who received which treatment, to reduce bias.

[0115] The changes in physiological and psychological indicators between the experimental group and the control group were compared through statistical analysis to evaluate the effect of the product.

[0116] 3. Observation indicators and detection methods

[0117] 1. Weight and body fat percentage: Use accurate weight scales and body fat meters to measure regularly. Record and analyze the downward trend of weight and body fat percentage.

[0118] 2. Muscle mass: Use bioelectrical impedance analysis (BIA) technology to measure muscle mass. Compare the changes in muscle mass between the experimental group and the control group during weight loss.

[0119] 3. Depression scale score: Use standardized depression scales and anxiety scales such as PHQ-9 and GAD-7 for psychological assessment. Record and analyze the changes in scale scores to evaluate the improvement effect of bergamot ingredients on depressive symptoms.

[0120] Statistical Analysis

[0121] 1. Use SPSS statistical software for data analysis.

[0122] 2. Measurement data were expressed as mean ± standard deviation, and independent sample t test was used for comparison between groups.

[0123] 3. Enumeration data were expressed as number of cases (percentage), and chi-square test was used for comparison between groups.

[0124] 4. The difference was considered statistically significant when bilateral P<0.05.

[0125] 5. Clinical trial results

[0126] Table 1 Clinical trial results 1

[0127] Grouping / Indicators Experimental Group 1 Control group P-value Mean ± SD of body weight change / kg -5.2±1.5 -1.5±0.8 <0.05 Body fat percentage change mean ± SD / % -4.5±1.2 -1.0±0.7 <0.05 Muscle mass change mean ± SD / kg -0.5±0.4 -0.5±0.3 ＞0.05 PHQ-9 / point mean ± SD -4.2±1.8 -0.5±1.0 <0.05 GAD-7 / point mean ± SD -5.7±1.9 -0.7±0.9 <0.05

[0128] Table 2 Clinical trial results 2

[0129] Grouping / Indicators Experimental Group 2 Control group P-value Mean ± SD of body weight change / kg -4.0±1.3 -1.5±0.8 ＜0.05 Body fat percentage change mean ± SD / % -3.0±1.1 -1.0±0.7 ＜0.05 Muscle mass change mean ± SD / kg -0.6±0.4 -0.5±0.3 ＞0.05 PHQ-9 / point mean ± SD -2.3±1.2 -0.5±1.0 <0.05 GAD-7 / point mean ± SD -3.1±0.3 -0.7±0.9 <0.05

[0130] Table 3 Clinical trial results 3

[0131] Grouping / Indicators Experimental Group 3 Control group P-value Mean ± SD of body weight change / kg -1.8±1.0 -1.5±0.8 ＞0.05 Body fat percentage change mean ± SD / % -1.5±0.9 -1.0±0.7 ＞0.05 Muscle mass change mean ± SD / kg -0.7±0.5 -0.5±0.3 ＞0.05 PHQ-9 / point mean ± SD -3.9±1.2 -0.5±1.0 ＜0.05 GAD-7 / point mean ± SD -5.2±1.1 -0.7±0.9 ＜0.05

[0132] As can be seen from Table 1, the body weight and body fat percentage of the subjects in the experimental group decreased significantly, and the decrease was greater than that in the blank control group. Among them, the weight loss effect of the subjects in the experimental group was more significant; the muscle mass of the subjects in the experimental group was effectively maintained during the weight loss process, while the muscle mass of the subjects in the control group decreased. This shows that the high-efficiency protein powder in the bergamot compound protein powder helps maintain muscle mass. The depression scale and anxiety scale scores of the subjects in the experimental group were significantly reduced after the intervention, and the decrease was greater than that in the control group, indicating that the bergamot compound protein powder of the present invention helps to improve the depressive symptoms of patients who lose weight. As can be seen from the results of Tables 2 and 3, the microencapsulated bergamot extract of the present invention has a good synergistic effect with other raw materials.

[0133] The present invention combines the liver-soothing and qi-regulating components of bergamot with high-efficiency protein powder to prepare a composite protein powder that has a significant effect on patients who are losing weight. The product can not only achieve the goal of losing weight and maintaining muscle mass, but also relieve the depression symptoms that may accompany the patient, and is a healthy food with broad application prospects.

[0134] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A bergamot compound protein powder, characterized in that: In parts by mass: 6-10 parts of bergamot extract microcapsules, 45-50 parts of isolated soy protein powder, 10-20 parts of polydextrose, 8-10 parts of resistant dextrin, 6-10 parts of soy peptide powder, 5-7 parts of tremella powder, 1-2 parts of edible salt, 0-2 parts of edible flavor, 1-3 parts of nutritional enhancer, 0.05-0.1 parts of sweetener and 1-2 parts of anti-caking agent.

2. A bergamot composite protein powder according to claim 1, characterized in that, The nutritional enhancer is at least one of concentrated milk protein powder, calcium caseinate and linseed oil microcapsule powder.

3. A bergamot composite protein powder according to claim 2, characterized in that, The nutritional enhancer comprises, by weight, 0.66 parts of concentrated milk protein powder, 0.66 parts of calcium caseinate, and 0.8 parts of linseed oil microcapsule powder.

4. A bergamot composite protein powder according to claim 1, characterized in that, The preparation method of the bergamot extract microcapsules comprises the following steps: S1. Preparation of bergamot extract: pre-treating bergamot and performing supercritical CO2 extraction, and drying the extract to obtain bergamot extract; S2. Microencapsulation of bergamot extract: dissolve sodium alginate in deionized water to obtain solution A, dissolve chitosan in acetic acid solution to obtain solution B, mix bergamot extract, emulsifier and water evenly to obtain solution C, then slowly drop solution C into solution A to obtain emulsion, then slowly drop the emulsion into solution B, filter, wash and dry after the reaction is completed to obtain bergamot extract microcapsules.

5. A bergamot composite protein powder according to claim 4, characterized in that, The extraction pressure of the supercritical CO2 extraction is 20-50 MPa, the extraction temperature is 35°C-60°C, and the extraction time is 1-4 hours.

6. A bergamot composite protein powder according to claim 4, characterized in that, The mass ratio of the bergamot extract to the sodium alginate and chitosan is 80:1:

1.

7. The method for preparing the bergamot composite protein powder according to any one of claims 1 to 6, characterized in that: The following steps are involved: The raw materials are weighed in proportion, and then the soy protein isolate powder and resistant dextrin are premixed by low-speed shearing, followed by adding bergamot extract microcapsules and polydextrose for a high-speed shearing mixing, and finally the remaining raw materials are added for a second high-speed shearing mixing. After all the raw materials are evenly mixed, bergamot composite protein powder is obtained.

8. An application of bergamot compound protein powder in weight loss and alleviating depression and anxiety symptoms, characterized in that: The bergamot composite protein powder is the bergamot composite protein powder according to any one of claims 1 to 6 or the bergamot composite protein powder prepared by the method according to claim 7.