Composition for treating hyperuricemia in chickens and preparation method thereof

Abstract

The present invention relates to the technical field of poultry and livestock disease prevention and control, and specifically to a composition for treating hyperuricemia in chickens and a preparation method thereof. The composition comprises 100-200 parts of chicory, 50-150 parts of Radix Sarcandrae, 0-1.5 parts of vitamin A, and 0-1.5 parts of vitamin D. The composition can be used to prepare a chicken gout drug. The preparation method comprises the following steps: adding water to chicory and Radix Sarcandrae, heating and extracting them twice, filtering and combining the extracts, concentrating and spray drying to obtain extract powder, dissolving vitamin A and vitamin D in ethanol for standby use, mixing the extract powder with auxiliary materials, and spraying the vitamin ethanol solution for granulation. The composition of the present invention adopts a combination of Chinese herbal medicine and vitamins, is safer and more reliable, has little effect on the growth and health of chickens, and is beneficial to industrial benefits. The raw materials used in the composition of the present invention are common, the preparation method is relatively simple, the cost is low, and it is easy to promote and apply to the prevention and treatment of hyperuricemia in chickens in the poultry industry.

Description

Technical Field

[0001] The invention relates to the technical field of poultry and livestock disease prevention and treatment, and in particular to a composition for treating chicken hyperuricemia and a preparation method thereof. Background Art

[0002] Hyperuricemia in chickens is the main cause of gout in chickens. Its mechanism is that excessive urate accumulates in the blood and cannot be quickly excreted from the body. The main reasons can be divided into two categories: one is excessive uric acid production in the body, and the other is uric acid excretion disorder in the body; and feeding a large amount of protein feed rich in nucleoprotein purine bases, such as soybeans, peas, fish meal, animal offal, etc., is the main factor of hyperuricemia in chickens. Its pathological characteristics are increased blood uric acid levels, and urate is deposited in joint capsules, articular cartilage, internal organs, renal tubules, ureters and other interstitial tissues. The main clinical manifestations of gout in chickens are anorexia, exhaustion, diarrhea, leg and wing joint swelling, slow movement, decreased egg production and increased mortality, which bring huge losses to the breeding industry. The relevant prevention methods mainly include providing low-protein, low-purine feed, supplementing vitamin A and vitamin D, and stopping the use of nephrotoxic drugs; the treatment method mainly includes feeding chickens with sodium bicarbonate and allopurinol, but these drugs may cause gastrointestinal reactions and metabolic alkalosis in chickens, affecting the growth and health of chickens and affecting industry profits. At present, there is no better treatment for hyperuricemia in chickens.

[0003] Chinese herbal medicine has a long history of treating gout and lowering uric acid. Patent document CN111617231A (application number 201911057802.5) discloses a uric acid-lowering and anti-gout composition and its preparation method and application, including the following raw materials in parts by weight: 6-20 parts of marine fish oligopeptides, 12-24 parts of Hovenia dulcis, 10-20 parts of chicory, 15-30 parts of dandelion, 5-15 parts of mango powder, 2-10 parts of acerola powder, 2-10 parts of monk fruit, and 0.2-2 parts of sweetener, which can well reduce blood uric acid levels and achieve anti-gout effects. Patent document CN113288925A (application number 202110804719.0) discloses a Chinese medicine composition for preventing and treating gout, which is prepared from chicory root, Poria cocos, and Cyathula officinalis, and has the effects of lowering uric acid, promoting uric acid excretion, and anti-inflammatory and analgesic effects. The above prescriptions are all human medicines. The dosage for chickens calculated according to the weight ratio is often not effective. In addition, the liver and kidney functions of chickens are weak, and the complex prescriptions make it impossible for them to metabolize and cause other diseases. Therefore, there are currently no reports on Chinese herbal medicine preparations specifically for the treatment of hyperuricemia in chickens. Finding safe, effective and low-cost drugs for the prevention and treatment of hyperuricemia in chickens is an urgent problem to be solved. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a composition for treating hyperuricemia in chickens and a preparation method thereof.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A composition for treating hyperuricemia in chickens is prepared from the following raw materials in parts by weight: 100-200 parts of chicory, 50-150 parts of Sarcandra scabra, 0-1.5 parts of vitamin A, and 0-1.5 parts of vitamin D.

[0007] Preferably according to the present invention, the composition for treating hyperuricemia in chickens is prepared using the following raw materials in parts by mass: 150 parts of chicory, 100 parts of Sarcandra scabra, 1 part of vitamin A, and 1 part of vitamin D.

[0008] Preferably according to the present invention, the potency of the above-mentioned vitamin A is 250,000 to 750,000 units / portion, and the potency of vitamin D is 25,000 to 75,000 units / portion.

[0009] More preferably, the potency of the above-mentioned vitamin A is 500,000 units / portion, and the potency of the above-mentioned vitamin D is 50,000 units / portion.

[0010] Application of the composition in preparing chicken gout medicine.

[0011] The method for preparing a chicken gout medicine using the above composition is characterized in that the steps are as follows:

[0012] (1) Add water to chicory and Radix Achyranthis Bidentatae, heat and extract twice, filter and combine the extracts;

[0013] (2) concentrating the extract and spray drying to obtain extract powder;

[0014] (3) dissolving vitamin A and vitamin D in ethanol to prepare a vitamin ethanol solution for later use;

[0015] (4) After the extract powder and the auxiliary materials are mixed evenly, the vitamin ethanol solution prepared in step (3) is sprayed into the mixture and granulated to obtain the product.

[0016] Preferably according to the present invention, in step (1), during the process of adding water for heating, the mass ratio of the medicinal material to water is: 1:(8-10).

[0017] Further preferably, during the first heating extraction process, the mass ratio of the medicinal material to water is 1:10, and during the second heating extraction process, the mass ratio of the medicinal material to water is 1:8.

[0018] According to the preferred embodiment of the present invention, in step (1), the time of the two heating extractions is: 0.5 to 1 h;

[0019] Further preferably, in step (1), the first heating extraction time is 1 hour, and the second heating extraction time is 0.5 hour.

[0020] Preferably, according to the present invention, in step (2), the concentration method is vacuum rotary evaporation, and the concentration temperature is 40-60°C.

[0021] Preferably, according to the present invention, in step (2), the relative density of the concentrated extract is 1.05 to 1.15.

[0022] Preferably according to the present invention, in step (3), the mass volume ratio of vitamin to ethanol is: 1:(5-15), unit: g / mL.

[0023] Preferably, according to the present invention, in step (4), the auxiliary material is one or more of dextrin, sugar or starch, and the mass ratio of the auxiliary material to the total amount of raw materials is 1:(3-5).

[0024] The pharmacological effects of the Chinese medicine used in the present invention are as follows:

[0025] Chicory: slightly bitter, salty, and cool; it has the effects of clearing the liver and promoting bile secretion, strengthening the stomach and digestion, and promoting diuresis and reducing swelling; it is used for damp-heat jaundice, stomach pain, loss of appetite, edema, and oliguria.

[0026] Radix Sarcandrae: bitter, pungent, flat; has the effects of clearing away heat, detoxifying, cooling blood, promoting blood circulation, removing spots and dispersing blood stasis, dispelling wind, removing dampness and dredging collaterals; used for blood-heat purpura, rheumatic arthralgia, traumatic injuries, limb numbness, fractures, dysmenorrhea in women, abdominal pain due to postpartum stasis, pneumonia, acute appendicitis, acute gastroenteritis, bacillary dysentery, cholecystitis, abscesses, stomatitis, and rheumatic arthralgia.

[0027] Hyperuricemia in chickens is caused by the accumulation of urate due to the disorder of purine metabolism in the chickens. The liver-clearing and gallbladder-promoting, diuretic and detumescent effects of chicory help promote the excretion of uric acid; Radix Lycopodiellae has the effects of clearing heat and cooling blood, dispelling wind and unblocking collaterals. It improves the body's blood circulation, assists the body's metabolic waste discharge, and relieves the deposition of urate. These two herbs are used as the main ingredients; vitamin A has the effect of protecting the mucosa, preventing mucosal damage and infection caused by gout, and vitamin D can regulate calcium and phosphorus metabolism, avoid aggravating the kidney burden due to abnormal calcium and phosphorus metabolism, and prevent the increased kidney burden from further affecting uric acid excretion. These two herbs play the role of auxiliary treatment. The combination of these herbs can synergistically enhance the efficacy.

[0028] Beneficial effects:

[0029] (1) The composition of the present invention has a significant effect of reducing uric acid in the blood, and is more effective for treating hyperuricemia in chickens. Compared with the existing drugs such as sodium bicarbonate and allopurinol used to treat hyperuricemia in chickens, the composition of the present invention uses a combination of Chinese herbal medicine and vitamins, which is safer and more reliable, has little effect on the growth and health of chickens, and is beneficial to industrial benefits.

[0030] (2) The raw materials used in the composition of the present invention are common, the preparation method is relatively simple, the cost is low, and it is easy to promote and apply to the prevention and treatment of hyperuricemia in chickens in the poultry industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of the swelling of the chicken's elbow joint caused by hyperuricemia;

[0032] Figure 2 This is a schematic diagram of the reduction of swelling in the elbow joint of a chicken's foot after administration of the gout drug prepared in Example 1. DETAILED DESCRIPTION

[0033] The present invention is described in detail below in conjunction with the embodiments and test examples. Obviously, the described embodiments are only embodiments of a part of the present invention, not all embodiments, and the protection scope of the present invention is not limited thereto. The test methods used in the following embodiments are conventional methods unless otherwise specified; the materials, reagents, etc. used are all commercially available materials and reagents unless otherwise specified. Among them, the vitamin A potency is 500,000 units / g, and the vitamin D potency is 50,000 units / g, both of which were purchased from Zhejiang Medicine Co., Ltd.

[0034] Example 1

[0035] A chicken gout medicine is prepared from the following raw materials in parts by weight: 150 parts of chicory, 100 parts of Sarcandra scabra, 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 62 parts of auxiliary materials (20 parts of dextrin and 42 parts of sucrose). The specific preparation steps are as follows:

[0036] (1) Take chicory and Radix Salviae Miltiorrhizae, add 10 times the weight of water, boil for 1 hour, filter to obtain the decoction, add 8 times the weight of water to the two medicinal materials, boil for 30 minutes, and filter to obtain the decoction;

[0037] (2) concentrating the extract and spray drying to obtain extract powder;

[0038] (3) Dissolving vitamin A and vitamin D in 20 parts of ethanol to prepare a vitamin ethanol solution for later use;

[0039] (4) After the extract powder and the auxiliary materials are mixed evenly, the vitamin ethanol solution prepared in step (3) is sprayed into the mixture and granulated to obtain the product.

[0040] Example 2

[0041] A chicken gout medicine is prepared from the following raw materials in parts by mass: 100 parts of chicory, 150 parts of Sarcandra scabra, 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 62 parts of auxiliary materials (20 parts of dextrin and 42 parts of sucrose), and granulated according to the steps of Example 1 to obtain the medicine.

[0042] Example 3

[0043] A composition for treating hyperuricemia in chickens is prepared from the following raw materials in parts by mass: 200 parts of chicory, 50 parts of Sarcandra sarmentosa, 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 62 parts of auxiliary materials (20 parts of dextrin and 42 parts of sucrose), and granulated according to the steps of Example 1 to obtain the composition.

[0044] Example 4

[0045] A chicken gout medicine is prepared from the following raw materials in parts by weight: 150 parts of chicory, 100 parts of Sarcandra sarmentosa, and 64 parts of auxiliary materials (21 parts of dextrin and 43 parts of sucrose). The specific steps are as follows: prepare the extract powder according to Example 1, mix it with the auxiliary materials, and spray ethanol into it for granulation.

[0046] Comparative Example 1

[0047] A chicken gout medicine is prepared from the following raw materials in parts by mass: 250 parts of chicory, 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 62 parts of auxiliary materials (20 parts of dextrin and 42 parts of sucrose), and granulated according to the steps of Example 1 to obtain the medicine.

[0048] Comparative Example 2

[0049] A chicken gout medicine is prepared from the following raw materials in parts by mass: 250 parts of Sarcandra scabra, 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 62 parts of auxiliary materials (20 parts of dextrin and 42 parts of sucrose). Granulation is performed according to the steps of Example 1 to obtain the medicine.

[0050] Comparative Example 3

[0051] A chicken gout medicine is prepared from the following raw materials by mass: 500,000 units of vitamin A (1 part), 50,000 units of vitamin D (1 part), and 98 parts of auxiliary materials (32 parts of dextrin and 66 parts of sucrose). The specific steps are: dissolving vitamin A and vitamin D in 20 parts of ethanol to obtain a vitamin ethanol solution; mixing the auxiliary materials, spraying the vitamin ethanol solution, and granulating to obtain the medicine.

[0052] Experimental Example 1

[0053] The therapeutic effect of the above composition on hyperuricemia in chickens

[0054] (1) Clinical symptoms: excretion of white, dilute urate feces, decreased food intake, lethargy, weight loss, slow movement, shortness of breath, or unsteady standing, swelling of the feet and elbow joints, such as Figure 1 shown.

[0055] (2) Blood biochemical indicators: The detection indicators and methods in "Serological Detection and Research on Experimental Hyperuricemia Chickens" were used, and a blood uric acid value greater than 476 μmol / L was used as the criterion for hyperuricemia.

[0056] (3) Administration: 2 g / head / day, mixed with feed.

[0057] 72-day-old chickens were selected, 15 in each group, and fed with high-protein feed for 2 weeks. The blood uric acid value was the main criterion, and the clinical symptoms were the secondary criterion. Chickens with blood uric acid values ​​greater than 476 μmol / L and similar body weight with more than one clinical symptom were selected as experimental chickens, a total of 90 chickens, divided into 6 groups. The specific administration method is shown in Table 1. The blood uric acid value was tested 7 days after medication and the symptoms were observed; high-protein feed was continuously fed during the experiment.

[0058] The results are shown in Table 1. The composition of the present invention has a significant effect of reducing blood uric acid and has a good therapeutic effect on gout in chickens. After 7 days of administration, despite continuous feeding of high-protein feed, the average blood uric acid value dropped to 302 μmol / L, which is far below the critical value of hyperuricemia. The swelling of the elbow joints of the feet was significantly reduced. Figure 2 As shown; Examples 2 and 3 show that when the dosage is consistent, compared with Example 1, the best effect is achieved when the ratio of chicory to Radix Sarcandrae is 3:2; Example 4 shows that chicory and Radix Sarcandrae can also significantly reduce uric acid levels without the effect of vitamins. Although vitamin A and vitamin D have little effect on symptom treatment, they have a certain auxiliary synergistic effect on lowering uric acid. Although Comparative Example 1 and Comparative Example 2 have the effect of lowering blood uric acid, they are not lower than the critical value of hyperuricemia blood uric acid, and the treatment effect is poor, indicating that the combined effect cannot be achieved by relying on only one of the drugs. Comparative Example 3 shows that vitamin A and vitamin D only play an auxiliary role and cannot be used for treatment.

[0059] Table 1. Results of the experimental study on the treatment of gout in chickens

[0060]

[0061]

[0062] Experimental Example 2

[0063] Example 1 Therapeutic effects of the composition on hyperuricemia in chickens, ducks and geese

[0064] Three animals of the same age, chicken, duck and goose, were selected, and high-protein feed was fed according to normal feeding conditions of each animal for two consecutive weeks. Animals with similar growth status and similar blood uric acid values ​​were selected as experimental animals, 6 of each type, and the precise administration of Example 1 was performed according to 1g / kg body weight per animal, and the administration was scheduled every day for 7 consecutive days. The blood uric acid values ​​before and after the test were measured and compared. As shown in Table 2, the present composition has the best therapeutic effect on high uric acid in the blood of chickens, with a decrease rate of 43.81%; followed by geese, with a decrease rate of 36.09%, and finally ducks, with a decrease rate of 25.57%. The present composition has a good therapeutic effect on hyperuricemia in chickens.

[0065] Table 2. Test results of the composition for reducing blood uric acid levels in different poultry

[0066]

[0067] The invention has a good effect of lowering uric acid in the blood and has a good therapeutic effect on gout in chickens. Although it has an effect on ducks and geese, the effect is not as good as that on chickens. This is because ducks and geese are aquatic organisms, so their nature is cool, while chickens are warm. This leads to differences in the causes of high uric acid in ducks, geese and chickens, so when taking the same medicine, the efficacy will also be different.

Claims

1. A composition for treating hyperuricemia in chickens, characterized in that: The invention is prepared from the following raw materials in parts by weight: 100-200 parts of chicory, 50-150 parts of Sarcandra sylvestris, 0-1.5 parts of vitamin A and 0-1.5 parts of vitamin D.

2. The composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 150 parts of chicory, 100 parts of Sarcandra scabra, 1 part of vitamin A and 1 part of vitamin D.

3. The composition according to any one of claims 1 to 2, characterized in that The potency of the vitamin A is 250,000 to 750,000 units / portion, and the potency of the vitamin D is 25,000 to 75,000 units / portion.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of a medicament for chicken gout.

5. A method for preparing a chicken gout medicine using the composition according to any one of claims 1 to 3, characterized in that: Here are the steps: (1) Add water to chicory and Radix Achyranthis Bidentatae, heat and extract twice, filter and combine the extracts; (2) concentrating the extract and spray drying to obtain extract powder; (3) dissolving vitamin A and vitamin D in ethanol to prepare a vitamin ethanol solution for later use; (4) After the extract powder and the auxiliary materials are mixed evenly, the vitamin ethanol solution prepared in step (3) is sprayed into the mixture and granulated to obtain the product.

6. The preparation method according to claim 5, characterized in that: In step (1), the mass ratio of medicinal materials to water is 1:(8-10), and the time for the two heating extractions is 0.5-1h.

7. The preparation method according to claim 5, characterized in that: In step (2), the concentration method is vacuum rotary evaporation, and the concentration temperature is 40-60°C.

8. The preparation method according to claim 5, characterized in that: In step (2), the relative density of the concentrated extract is 1.05 to 1.

15.

9. The preparation method according to claim 5, characterized in that: In step (3), the mass volume ratio of the total amount of vitamins to ethanol is: 1:(5-15), unit: g / mL.

10. The preparation method according to claim 5, characterized in that: In step (4), the auxiliary material is one or more of dextrin, sugar or starch, and the mass ratio of the auxiliary material to the total amount of raw materials is 1:(3-5).

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