A lipid-lowering tea bag and its preparation method

Abstract

The invention provides a blood lipid lowering tea bag and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The blood lipid lowering tea bag of the invention comprises: hawthorn, gynostemma pentaphyllum, bu za leaf, honeysuckle, kuding tea, pollen and liquorice. Among them, hawthorn can help digestion and invigorate the stomach, promote the digestion and decomposition of fat, gynostemma pentaphyllum can clear turbidity and reduce lipid, inhibit the differentiation of adipocytes and the synthesis of lipids, and they are all monarch drugs; bu za leaf can help digestion and resolve stagnation, and honeysuckle can clear heat and detoxify, and they are minister drugs; kuding tea can disperse wind and heat, clear the head, regulate body fluid metabolism, pollen can remove blood stasis and stop bleeding, and improve blood circulation, and they are adjuvant drugs; liquorice can harmonize various drugs and is a guiding drug. Various drugs are used together to synergistically achieve the effect of lowering blood lipids. The results of animal experiments show that taking the tea bag of the invention can significantly reduce the cholesterol, triglyceride and low-density lipoprotein cholesterol levels of mice, and increase the high-density lipoprotein cholesterol level, indicating that the tea bag of the invention has an excellent blood lipid lowering effect.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and particularly relates to a lipid-lowering tea bag and a preparation method thereof. Background Art

[0002] With the change of modern lifestyle, affected by factors such as high-calorie diet, lack of exercise, and long-term mental stress, the incidence of hyperlipidemia shows a significant upward trend globally. Hyperlipidemia refers to a metabolic disorder syndrome in which the levels of lipid components in the blood, such as cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C), increase, while the level of high-density lipoprotein cholesterol (HDL-C) decreases. A long-term hyperlipidemic state will lead to the formation of atherosclerosis, causing the blood vessel wall to thicken, harden, and lose elasticity, and then triggering a series of serious cardiovascular and cerebrovascular diseases, such as coronary heart disease, myocardial infarction, stroke, etc. These diseases not only seriously affect the quality of life of patients, but also have a high disability rate and mortality rate, bringing a heavy medical burden to families and society. According to relevant statistical data, more than 70% of the deaths from cardiovascular diseases are closely related to hyperlipidemia.

[0003] At present, the commonly used lipid-lowering drugs mainly include chemically synthesized drugs such as statins, fibrates, and niacin. Statins are the most widely used cholesterol-lowering drugs, which reduce the cholesterol level in the blood by inhibiting the key enzyme in the cholesterol synthesis process. Although their lipid-lowering effect is significant, long-term use may cause some adverse reactions, such as liver damage, rhabdomyolysis, etc., and some patients have the situation of statin intolerance. Fibrates are mainly used to reduce the triglyceride level, but they may also cause side effects such as gastrointestinal discomfort and abnormal liver and kidney functions. Niacin drugs often cause adverse reactions such as facial flushing, skin itching, and hyperglycemia while lowering blood lipids, which limits their wide application in clinical practice.

[0004] Traditional Chinese medicine has a long history and rich experience in the treatment of metabolic diseases. Traditional Chinese medicine has relatively high safety and fewer adverse reactions, and is more suitable for long-term use. Developing an efficient and safe lipid-lowering traditional Chinese medicine composition has broad market prospects and important significance, and is expected to provide a new treatment option for hyperlipidemia patients and meet the growing medical needs. Summary of the Invention

[0005] In view of the above technical problems, the present invention provides a lipid-lowering tea bag and a preparation method thereof, and the tea bag provided by the present invention has excellent lipid-lowering effect.

[0006] In order to achieve the above invention purpose, the present invention provides the following technical solutions:

[0007] The present invention provides a traditional Chinese medicine composition for reducing blood lipid, comprising the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of microcos paniculata leaves, 20-30 parts of lonicera confusa, 3-8 parts of kudingcha, 1-3 parts of cattail pollen, and 1-3 parts of liquorice.

[0008] The present invention also provides the application of the above traditional Chinese medicine composition in the preparation of a medicine for reducing blood lipid.

[0009] The present invention also provides a tea bag for reducing blood lipid, which is made from the above traditional Chinese medicine composition.

[0010] The present invention also provides a preparation method of the above tea bag for reducing blood lipid, comprising: respectively crushing hawthorn, gynostemma pentaphyllum, microcos paniculata leaves, lonicera confusa, kudingcha, and liquorice; mixing hawthorn and gynostemma pentaphyllum with water, enzymatically hydrolyzing them successively with hemicellulase and nattokinase, fermenting with a composite microorganism, sterilizing, and filtering to obtain a fermentation broth; mixing microcos paniculata leaves and lonicera confusa, ultrasonically extracting them with an ethanol solution, and filtering to obtain a filtrate; mixing the fermentation broth and the filtrate, and spray-drying to obtain a dry powder; mixing kudingcha, cattail pollen, liquorice, and the dry powder, sterilizing, and filling them into a tea bag to obtain the tea bag for reducing blood lipid; the composite microorganism is composed of monascus purpureus and schizophyllum commune according to a weight ratio of 1-3:2-6.

[0011] Preferably, the ratio of the total weight of hawthorn and gynostemma pentaphyllum to the weight of water is 1:5-8.

[0012] Preferably, the addition amount of the hemicellulase is 0.5‰-1.3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the hemicellulase enzymatic hydrolysis is 50-60°C, the pH value is 4-6, and the time is 1-3 h; the addition amount of the nattokinase is 1‰-3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the nattokinase enzymatic hydrolysis is 45-55°C, the pH value is 7-9, and the time is 2-4 h.

[0013] Preferably, the inoculation amount of the composite microorganism is 4‰-8‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the fermentation is 25-35°C, the pH value is 5-6.5, and the time is 5-10 d.

[0014] Preferably, the volume percentage content of the ethanol solution is 45%-65%, and the ratio of the total mass of microcos paniculata leaves and lonicera confusa to the mass of the ethanol solution is 1:5-8.

[0015] Preferably, the power of the ultrasonic wave is 200-300 W.

[0016] Preferably, the material-liquid ratio of the extraction is 1:5-8 g / ml, the temperature is 40-50°C, the time is 30-40 min, and the number of extraction times is 2-4 times.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides a traditional Chinese medicine composition for reducing blood lipid, comprising: hawthorn, gynostemma pentaphyllum, microcos paniculata leaves, lonicera confusa, glossy privet leaf, cattail pollen and liquorice. Among them, hawthorn promotes digestion and strengthens the stomach, and promotes the digestion and decomposition of fat. Gynostemma pentaphyllum reduces turbidity and lipid, and inhibits the differentiation of fat cells and the synthesis of lipids. They are the monarch drugs together; Microcos paniculata leaves promote digestion and relieve stagnation, and lonicera confusa clears heat and detoxifies, and they are the ministerial drugs; Glossy privet leaf disperses wind-heat, clears the head, regulates the metabolism of body fluids, and cattail pollen removes stasis and stops bleeding, improving blood circulation. They are the adjuvant drugs; Liquorice harmonizes all the drugs and is the messenger drug. All the drugs are used in combination to synergistically achieve the effect of reducing blood lipid. Detailed implementation mode

[0019] The present invention provides a traditional Chinese medicine composition for reducing blood lipid, comprising the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of microcos paniculata leaves, 20-30 parts of lonicera confusa, 3-8 parts of glossy privet leaf, 1-3 parts of cattail pollen and 1-3 parts of liquorice.

[0020] The hawthorn in the present invention promotes digestion and strengthens the stomach, contains various organic acids, such as crataegolic acid, citric acid, etc., can increase the secretion of digestive enzymes in the stomach, and promote the digestion and decomposition of fat. At the same time, hawthorn can also regulate lipid metabolism and reduce the contents of triglyceride and cholesterol in the blood. In the traditional Chinese medicine composition, hawthorn plays a leading and key role in the regulation of blood lipid and is the monarch drug.

[0021] The gynostemma pentaphyllum in the present invention reduces turbidity and lipid, can inhibit the differentiation of fat cells and the synthesis of lipids, reduce low-density lipoprotein cholesterol (LDL-C), and increase high-density lipoprotein cholesterol (HDL-C), and plays an important role in the whole blood lipid regulation process. In the traditional Chinese medicine composition, it acts as the monarch drug together with hawthorn.

[0022] The microcos paniculata leaves in the present invention have the effect of promoting digestion and relieving stagnation, and can assist the monarch drug hawthorn to strengthen the digestion of fat in food. Microcos paniculata leaves can inhibit cholesterol absorption, regulate lipid metabolism, and promote excretion, thereby indirectly playing a role in reducing blood lipid. In the traditional Chinese medicine composition, it is used as the ministerial drug to assist the monarch drug hawthorn and enhance its effect of promoting digestion and reducing blood lipid.

[0023] The lonicera confusa in the present invention clears heat and detoxifies, and has antioxidant and anti-inflammatory properties. Patients with hyperlipidemia often have oxidative stress and inflammatory reactions in their bodies, and these reactions will interfere with lipid metabolism. Lonicera confusa can reduce inflammatory reactions and scavenge free radicals, thereby creating a good internal environment for the normal metabolism of blood lipid. Cooperating with the monarch drug, it assists in reducing blood lipid from the perspective of improving the overall state of the body and plays the role of the ministerial drug.

[0024] The kudingcha of the present invention can disperse wind-heat, clear the head, and regulate body fluid metabolism. In the traditional Chinese medicine composition, kudingcha can prevent the dryness and heat that may be caused by other drugs in the formula (such as hawthorn, etc.). At the same time, kudingcha is beneficial to the cardiovascular system, can regulate blood lipids, and play an auxiliary role in reducing blood lipids. Therefore, it is an adjuvant drug to enhance the blood lipid-lowering effect of the whole formula and harmonize the properties of the drugs.

[0025] The puhuang of the present invention has the efficacy of promoting blood circulation to remove blood stasis and stopping bleeding. In the case of abnormal blood lipids, the blood viscosity increases, and it is easy to present a blood stasis state. Puhuang can improve the blood stasis state, promote blood circulation, and enable the lipids in the blood to be better transported and metabolized. In the traditional Chinese medicine composition, puhuang is used as an adjuvant drug to assist the monarch drug and ministerial drug to improve blood circulation, thereby facilitating the regulation of blood lipids.

[0026] The licorice of the present invention has the effect of harmonizing various drugs and can relieve the strong properties of other drugs. It is a guiding drug. For example, drugs such as hawthorn and gynostemma pentaphyllum have relatively strong medicinal effects. Licorice can enable them to synergistically act better with each other and avoid possible conflicts between drugs. At the same time, licorice itself also has certain pharmacological effects, such as anti-inflammatory and anti-allergic effects, which also help to improve the overall state of the body and are beneficial to the regulation of blood lipids.

[0027] In the traditional Chinese medicine composition of the present invention, hawthorn promotes digestion and invigorates the stomach, and promotes the digestion and decomposition of fat. Gynostemma pentaphyllum reduces turbidity and lipid, and inhibits the differentiation of fat cells and the synthesis of lipids. They are both monarch drugs; microcos paniculata leaf promotes digestion and relieves food retention, and honeysuckle flower clears heat and detoxifies. They are ministerial drugs; kudingcha disperses wind-heat, clears the head, and regulates body fluid metabolism. Puhuang promotes blood circulation to remove blood stasis and improves blood circulation. They are adjuvant drugs; licorice harmonizes various drugs and is a guiding drug. The combination of various drugs synergistically achieves the efficacy of reducing blood lipids.

[0028] The present invention also provides the application of the above traditional Chinese medicine composition in the preparation of blood lipid-lowering drugs.

[0029] The present invention also provides a tea bag for reducing blood lipids, which is made of the above traditional Chinese medicine composition.

[0030] The present invention also provides a preparation method of the above blood lipid-lowering tea bag, including: respectively crushing hawthorn, gynostemma pentaphyllum, microcos paniculata leaf, honeysuckle flower, kudingcha and licorice; mixing hawthorn and gynostemma pentaphyllum with water, enzymatically hydrolyzing them with hemicellulase and nattokinase in sequence, fermenting with a composite microorganism, and filtering to obtain a fermentation broth; mixing microcos paniculata leaf and honeysuckle flower, ultrasonically extracting them with an ethanol solution, and filtering to obtain a filtrate; mixing the fermentation broth and the filtrate, and performing spray drying to obtain a dry powder; mixing kudingcha, puhuang, licorice and the dry powder, and packing them into a tea bag to obtain the blood lipid-lowering tea bag; the composite microorganism is composed of monascus purpureus and schizophyllum commune according to a weight ratio of 1-3:2-6.

[0031] The weight ratio of the total weight of hawthorn and gynostemma pentaphyllum to the weight of water in the present invention is preferably 1:5 - 8, and more preferably 1:6.

[0032] The enzyme activity of the hemicellulase in the present invention is preferably 40,000 - 80,000 U / g, more preferably 50,000 U / g, and the addition amount is preferably 0.5‰ - 1.3‰ of the total weight of hawthorn and gynostemma pentaphyllum, more preferably 1‰. The temperature for the hemicellulase hydrolysis is preferably 50 - 60°C, more preferably 55°C, the pH value is preferably 4 - 6, more preferably 5, and the time is preferably 1 - 3 h, more preferably 2 h; the enzyme activity of nattokinase is preferably 10,000 - 30,000 U / g, more preferably 20,000 U / g, and the addition amount is preferably 1‰ - 3‰ of the total weight of hawthorn and gynostemma pentaphyllum, more preferably 2‰. The temperature for the nattokinase hydrolysis is preferably 45 - 55°C, more preferably 50°C, the pH value is preferably 7 - 9, more preferably 8, and the time is preferably 2 - 4 h, more preferably 3 h. In the present invention, hawthorn and gynostemma pentaphyllum are first hydrolyzed by hemicellulase to destroy the cell wall structure, promote the dissolution and transformation of active ingredients, and then hydrolyzed by nattokinase, which can more significantly regulate blood lipid metabolism and synergistically achieve the effect of reducing blood lipid.

[0033] The monascus purpureus in the composite microorganism of the present invention is Monascus purpureus Monascus purpureus CICC ® 5046, and the viable count is 3 billion - 8 billion CFU / g, more preferably 5 billion CFU / g; the schizophyllum commune in the composite microorganism is Schizophyllum commune Schizophyllum commune CICC ® 2592, and the viable count is 8 billion - 12 billion CFU / g, more preferably 10 billion CFU / g. Monascus purpureus can inhibit cholesterol synthesis, reduce triglyceride and low-density lipoprotein, improve lipid metabolism, and has the effect of reducing blood lipid; the fermentation of Schizophyllum commune can produce schizophyllan, which can inhibit the activity of fatty acid synthase and reduce the synthesis of fatty acids. In the present invention, Monascus purpureus and Schizophyllum commune are used to ferment hawthorn and gynostemma pentaphyllum together, which has a synergistic effect and achieves a more significant blood lipid reduction effect.

[0034] The composite microorganism of the present invention is preferably composed of Monascus purpureus and Schizophyllum commune in a weight ratio of 1 - 3:2 - 6, more preferably 2:5; the inoculation amount of the composite microorganism is preferably 4‰ - 8‰ of the total weight of hawthorn and gynostemma pentaphyllum, more preferably 5‰. The temperature for fermentation is preferably 25 - 35°C, more preferably 30°C, the pH value is preferably 5 - 6.5, more preferably 6, and the time is preferably 5 - 10 d, more preferably 8 d.

[0035] The volume percentage content of the ethanol solution in the present invention is preferably 45%-65%, more preferably 55%. The mass ratio of the total mass of Microcos paniculata leaves and Lonicera confusa to the mass of the ethanol solution is preferably 1:5-8, more preferably 1:6. The power of the ultrasonic treatment is preferably 200-300W, more preferably 260W. The solid-liquid ratio of the extraction is preferably 1:5-8g / ml, more preferably 1:6g / ml. The temperature is preferably 40-50°C, more preferably 45°C. The time is preferably 30-40min, more preferably 35min. The number of extraction times is preferably 2-4 times, more preferably 3 times.

[0036] The present invention performs ethanol ultrasonic extraction on Microcos paniculata leaves and Lonicera confusa, which can accelerate the dissolution of active ingredients in traditional Chinese medicine and improve the efficacy of reducing blood lipid.

[0037] Each tea bag of the traditional Chinese medicine in the present invention weighs 3g and is brewed with boiling water once a day.

[0038] In the present invention, unless otherwise specified, all raw material components are commercially available products well-known to those skilled in the art.

[0039] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the embodiments in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0040] In the following embodiments, unless otherwise specified, all are conventional methods.

[0041] In the following embodiments, the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0042] The Monascus purpureus involved in the embodiment is Monascus purpureus Monascus purpureus CICC ® 5046, the Schizophyllum commune is Schizophyllum commune Schizophyllum commune CICC ® 2592.

[0043] Example 1

[0044] Preparation of blood lipid-reducing tea bag

[0045] (1) Respectively pulverize 75 parts by weight of hawthorn, 60 parts by weight of Gynostemma pentaphyllum, 40 parts by weight of Microcos paniculata leaves, 25 parts by weight of Lonicera confusa, 5 parts by weight of Ilex kudingcha and 2 parts by weight of licorice to a particle size of 100 mesh;

[0046] (2) Mix hawthorn, gynostemma pentaphyllum with water (the weight of water is 6 times the total weight of hawthorn and gynostemma pentaphyllum), add hemicellulase accounting for 1‰ of the total weight of hawthorn and gynostemma pentaphyllum (enzyme activity is 50,000 U / g), enzymolyze at 55°C and pH 5 for 2 h, then add nattokinase accounting for 2‰ of the total weight of hawthorn and gynostemma pentaphyllum (enzyme activity is 20,000 U / g), enzymolyze at 50°C and pH 8 for 3 h, raise the temperature to 85°C to inactivate the enzyme for 15 min. After cooling to room temperature, inoculate with composite microorganisms accounting for 5‰ of the total weight of hawthorn and gynostemma pentaphyllum (composed of monascus purpureus with a viable count of 5 billion CFU / g and schizophyllum commune with a viable count of 10 billion CFU / g in a weight ratio of 2:5), ferment at 30°C and pH 6 for 8 d, sterilize, filter to obtain the fermentation broth;

[0047] (3) Mix microcos paniculata leaves and lonicera confusa with an ethanol solution of 55% by volume (the weight of the ethanol solution is 6 times the total weight of microcos paniculata leaves and lonicera confusa), extract at an ultrasonic power of 260 W and 45°C for 35 min, filter, and extract 2 more times according to the above steps, and combine the filtrates;

[0048] (4) After mixing the fermentation broth and the filtrate, sterilize at 121°C for 15 min, and spray dry at 100°C until the water content is 4% to obtain the dry powder;

[0049] (5) Mix the dry powder, kudingcha, licorice and 2 parts by weight of typha pollen, sterilize, and pack into tea bags to obtain the anti - hyperlipidemia tea bags.

[0050] Example 2

[0051] Preparation of anti - hyperlipidemia tea bags

[0052] (1) Respectively crush 70 parts by weight of hawthorn, 50 parts by weight of gynostemma pentaphyllum, 35 parts by weight of microcos paniculata leaves, 20 parts by weight of lonicera confusa, 3 parts by weight of kudingcha and 1 part by weight of licorice to a particle size of 80 mesh;

[0053] (2) Mix hawthorn, gynostemma pentaphyllum with water (the weight of water is 5 times the total weight of hawthorn and gynostemma pentaphyllum), add hemicellulase accounting for 0.5‰ of the total weight of hawthorn and gynostemma pentaphyllum (enzyme activity is 40,000 U / g), enzymolyze at 50°C and pH 4 for 1 h, then add nattokinase accounting for 1‰ of the total weight of hawthorn and gynostemma pentaphyllum (enzyme activity is 10,000 U / g), enzymolyze at 45°C and pH 7 for 2 h, raise the temperature to 85°C to inactivate the enzyme for 15 min. After cooling to room temperature, inoculate with composite microorganisms accounting for 4‰ of the total weight of hawthorn and gynostemma pentaphyllum (composed of monascus purpureus with a viable count of 3 billion CFU / g and schizophyllum commune with a viable count of 8 billion CFU / g in a weight ratio of 1:2), ferment at 25°C and pH 5 for 10 d, sterilize, filter to obtain the fermentation broth;

[0054] (3) Mix Patrinia leaf and Lonicera hypoglauca Miq. with an ethanol solution at a volume percentage of 45% (the weight of the ethanol solution is 5 times the total weight of Patrinia leaf and Lonicera hypoglauca Miq.), extract at a ultrasonic power of 200 W and a temperature of 40 °C for 40 min, filter, extract once again according to the above steps, and combine the filtrates;

[0055] (4) After mixing the fermentation broth and the filtrate, sterilize at 121 °C for 15 min, and spray-dry at 80 °C until the water content is 8% to obtain a dry powder;

[0056] (5) After mixing the dry powder, Ilex kudingcha C. J. Tsai, liquorice and 1 part by weight of Typha angustata Bory et Chaub., sterilize and pack into tea bags to obtain the lipid-lowering tea bags.

[0057] Example 3

[0058] Preparation of lipid-lowering tea bags

[0059] (1) Respectively crush 80 parts by weight of hawthorn, 70 parts by weight of Gynostemma pentaphyllum (Thunb.) Makino, 45 parts by weight of Patrinia leaf, 30 parts by weight of Lonicera hypoglauca Miq., 8 parts by weight of Ilex kudingcha C. J. Tsai and 3 parts by weight of liquorice to a particle size of 120 mesh;

[0060] (2) Mix hawthorn and Gynostemma pentaphyllum (Thunb.) Makino with water (the weight of water is 8 times the total weight of hawthorn and Gynostemma pentaphyllum (Thunb.) Makino), add 1.3‰ of hemicellulase (enzyme activity is 80,000 U / g) based on the total weight of hawthorn and Gynostemma pentaphyllum (Thunb.) Makino, carry out enzymatic hydrolysis at 60 °C and a pH value of 6 for 3 h, then add 3‰ of nattokinase (enzyme activity is 30,000 U / g) based on the total weight of hawthorn and Gynostemma pentaphyllum (Thunb.) Makino, carry out enzymatic hydrolysis at 55 °C and a pH value of 9 for 4 h, raise the temperature to 85 °C to inactivate the enzyme for 15 min, cool to room temperature, inoculate 8‰ of composite microorganisms (composed of Monascus purpureus Went with a viable count of 8 billion CFU / g and Schizophyllum commune Fr. with a viable count of 12 billion CFU / g in a weight ratio of 3:6) based on the total weight of hawthorn and Gynostemma pentaphyllum (Thunb.) Makino, ferment at 35 °C and a pH value of 6.5 for 5 d, sterilize and filter to obtain the fermentation broth;

[0061] (3) Mix Patrinia leaf and Lonicera hypoglauca Miq. with an ethanol solution at a volume percentage of 65% (the weight of the ethanol solution is 8 times the total weight of Patrinia leaf and Lonicera hypoglauca Miq.), extract at a ultrasonic power of 300 W and a temperature of 50 °C for 30 min, filter, extract 3 times again according to the above steps, and combine the filtrates;

[0062] (4) After mixing the fermentation broth and the filtrate, sterilize at 121 °C for 15 min, and spray-dry at 110 °C until the water content is 3% to obtain a dry powder;

[0063] (5) After mixing the dry powder, Ilex kudingcha C. J. Tsai, liquorice and 3 parts by weight of Typha angustata Bory et Chaub., sterilize and pack into tea bags to obtain the lipid-lowering tea bags.

[0064] Comparative Example 1

[0065] The specific implementation method is the same as that of Example 1, except that the enzymatic hydrolysis order of hemicellulase and nattokinase is reversed.

[0066] Comparative Example 2

[0067] The specific implementation method is the same as that of Example 1, except that the complex microorganism is replaced by a complex microorganism composed of Bacillus subtilis Bacillus subtilis CICC ® 10148 with a viable count of 5 billion CFU / g and Saccharomyces cerevisiae Saccharomyces cerevisiae CICC ® 1423 in a weight ratio of 2:5.

[0068] Comparative Example 3

[0069] The specific implementation method is the same as that of Example 1, except that the ethanol solution is replaced by an aqueous solution.

[0070] Comparative Example 4

[0071] The specific implementation method is the same as that of Example 1, except that hawthorn and Gynostemma pentaphyllum are not added.

[0072] Comparative Example 5

[0073] The specific implementation method is the same as that of Example 1, except that Microcos paniculata and Lonicera confusa are not added.

[0074] Experimental Example 1

[0075] Animal experiment

[0076] 100 4-week-old Kunming mice, 50 males and 50 females, weighing 15 - 20 g. After being fed with the adaptive basal diet for one week, they were evenly divided into 10 groups, with 10 mice in each group. There were no significant differences in the number of males and females, body weight, growth status, etc. among the groups. During the experiment, the mice in each group were allowed to eat and drink freely. The specific grouping is shown in Table 1. Among them, the high-fat diet is 85% basal diet + 10% lard + 1% cholesterol + 4% protein powder (the percentage content is the mass percentage).

[0077] Table 1 Experimental grouping and feeding scheme

[0078] Group Scheme Blank group Gavage with basal diet + 0.4 mL normal saline Model group Gavage with high-fat diet + 0.4 mL normal saline Example 1 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Example 1 per kg body weight Example 2 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Example 2 per kg body weight Example 3 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Example 3 per kg body weight Comparative Example 1 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Comparative Example 1 per kg body weight Comparative Example 2 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Comparative Example 2 per kg body weight Comparative Example 3 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Comparative Example 3 per kg body weight Comparative Example 4 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Comparative Example 4 per kg body weight Comparative Example 5 Gavage with high-fat diet + 0.4 g of the Chinese medicine composition of Comparative Example 5 per kg body weight

[0079] According to the feeding scheme in Table 1, gavage was performed. After 8 weeks of gavage, blood was taken from the tails of the mice in each group to measure the contents of cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in the serum. The specific results are shown in Table 2.

[0080] Table 2 Serum TC, TG, HDL-C, and LDL-C levels of mice in each group

[0081] Group Total cholesterol TC (mmol / L) Triglyceride TG (mmol / L) HDL-C (mmol / L) LDL-C (mmol / L) Blank group 2.05 0.93 0.62 1.01 Model group 4.36 1.65 0.46 3.15 Example 1 2.96 0.84 0.93 1.65 Example 2 3.07 0.88 0.90 1.77 Example 3 3.12 0.90 0.87 1.84 Comparative Example 1 3.39 0.91 0.83 2.15 Comparative Example 2 3.46 0.94 0.79 2.24 Comparative Example 3 3.34 0.92 0.75 2.17 Comparative Example 4 3.74 1.24 0.61 2.57 Comparative Example 5 3.57 1.18 0.68 2.35

[0082] From the data in Table 2, the cholesterol in the blank group was significantly higher than that in the model group, indicating that the high-fat mouse model was successfully established. From the data of the model group and Examples 1-3, it can be seen that taking the tea bags of Examples 1-3 of the present invention can significantly reduce the levels of cholesterol, triglyceride and low-density lipoprotein cholesterol in mice, and increase the level of high-density lipoprotein cholesterol, indicating that the tea bags of the present invention have excellent lipid-lowering effects. From the data of Example 1 and Comparative Examples 1-2, it can be seen that the enzymolysis sequence of the monarch drug and the types of complex microorganisms affect the lipid-lowering effect; from the data of Example 1 and Comparative Example 3, it can be seen that the change of the solvent of the ministerial drug also has an impact on the lipid-lowering effect of the tea bag; from the data of Example 1 and Comparative Examples 4-5, it can be seen that the monarch drug and the ministerial drug have a significant impact on the lipid-lowering effect of the tea bag.

[0083] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A tea bag for lowering blood lipids, characterized in that: The invention comprises the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of buzia leaves, 20-30 parts of honeysuckle, 3-8 parts of kudingcha, 1-3 parts of pollen cattail and 1-3 parts of liquorice; The preparation method of the tea bag comprises: respectively crushing hawthorn, gynostemma pentaphyllum, buzia leaf, honeysuckle, kudingcha and licorice; mixing hawthorn and gynostemma pentaphyllum with water, enzymolyzing with hemicellulase and nattokinase in turn, fermenting with composite microorganisms, sterilizing, filtering, and obtaining fermentation liquid; mixing buzia leaf and honeysuckle, ultrasonically extracting with ethanol solution, filtering, and obtaining filtrate; mixing the fermentation liquid and the filtrate, and spray drying to obtain dry powder; mixing kudingcha, pollen pollen, licorice and the dry powder, sterilizing, and putting into tea bags to obtain the lipid-lowering tea bag; the composite microorganisms are composed of purple red yeast rice and schizophyllum in a weight ratio of 1-3:2-6.

2. The tea bag according to claim 1, characterized in that The weight ratio of the total weight of the hawthorn and Gynostemma pentaphyllum to the weight of water is 1:5-8.

3. The tea bag according to claim 1, characterized in that The amount of hemicellulase added is 0.5‰-1.3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the hemicellulase enzymolysis is 50-60°C, the pH value is 4-6, and the time is 1-3h; the amount of nattokinase added is 1‰-3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the nattokinase enzymolysis is 45-55°C, the pH value is 7-9, and the time is 2-4h.

4. The tea bag according to claim 1, characterized in that The inoculation amount of the composite microorganism is 4‰-8‰ of the total weight of hawthorn and gynostemma pentaphyllum, the fermentation temperature is 25-35°C, the pH value is 5-6.5, and the fermentation time is 5-10 days.

5. The tea bag according to claim 1, characterized in that The volume percentage of the ethanol solution is 45%-65%, and the mass ratio of the total mass of the Buza leaves and honeysuckle to the ethanol solution is 1:5-8.

6. The tea bag according to claim 1, characterized in that The power of the ultrasound is 200-300W.

7. The tea bag according to claim 1, characterized in that The material-liquid ratio of the extraction is 1:5-8 g / ml, the temperature is 40-50° C., the time is 30-40 min, and the number of extractions is 2-4 times.