A sample collection device for coccidia oocyst detection and a sample detection method

Abstract

The invention discloses a sample collection device for coccidia oocyst detection and a sample detection method, wherein the sample collection device comprises a sampling rod, a collecting tube, a rotating bin and a transfer assembly, wherein at least one collecting groove is arranged on the peripheral wall of the lower end of the sampling rod, a recovery groove is arranged on the upper part of the collecting groove, a closed side plate which can move up and down is arranged in the recovery groove, a storage channel is arranged at a position of the lower end of the sampling rod corresponding to the collecting groove, the rotating bin comprises two upper and lower rotating circles, the rotating circles can rotate with the central axis of the sampling rod as the rotating axis, a plurality of placement cavities arranged in a circumferential array are arranged in the rotating circles, a connecting window is arranged at a position of the side wall of the sampling rod corresponding to the rotating bin, the placement cavity has a channel extending and opening toward the side wall of the sampling rod, a gap is arranged between the upper and lower rotating circles, a telescopic chuck is installed on the outside of the sampling rod, the telescopic chuck can be extended to pass through the gap between the rotating circles and probe into the placement cavity, so as to grab the collection tube and move it from the placement cavity through the connecting window into the sampling rod.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a sample collection device for coccidia oocyst detection and a sample detection method. Background Art

[0002] Coccidioidal monitoring is a diagnostic and preventive measure for coccidiosis, a parasitic disease caused by one or more coccidia, which is common in poultry and livestock, especially in intensive farming environments. Monitoring and control of coccidiosis is crucial to reducing economic losses in the farming industry.

[0003] Isospora suis destroys the intestinal villi and causes indigestion and infection with other intestinal pathogens, such as clostridial enteritis and Escherichia coli. Current methods for detecting coccidia include: 1. Direct microscopy: This is a simple and rapid detection method, but because the size of the oocysts of coccidia is (20-23)*(17-19)μm and they do not have micropyles, the value of direct microscopy of fecal smears is very limited, the detection rate is not high, and it is difficult to identify oocysts; 2. Salt-sugar solution flotation method: (100mL saturated saline solution + 50g sugar, density is 1.226) This method has a high detection rate and is the most commonly used detection method, but due to the presence of fat particles, it will interfere with the detection of oocysts. This method requires a little experience to have a faster and more accurate detection rate, but it is relatively the easiest method to master; 3. Teleman method: Also known as centrifugation, it is a more effective method for detecting oocysts because ether can remove fat substances in feces. However, it is also relatively cumbersome in the actual operation process; 4. PCR method: If it is determined whether it is an infection with Isospora, sporulation must be carried out, which takes a long time. The PCR detection method can quickly detect whether it is infected with Isospora suis, but the steps are cumbersome and the cost is high; 5. Indirect ELISA method: This test takes highly purified, fresh and impurity-free sporulated oocysts of Isospora suis, prepares oocyst homogenate as coating antigen to establish an indirect ELISA method, which overcomes the shortcomings of direct microscopy, such as time-consuming, labor-intensive and low detection rate, but the cost is high and the experimental conditions are required to be high. It can only be used as a method for the establishment of Isospora suis.

[0004] No matter which method is used to detect the sample, the technicians need to collect and process the feces sample first. The publication number CN214907288U discloses a feces sampling device for animal husbandry and veterinary medicine, including a sampling tube and a sampling rod installed in the sampling tube. A retaining ring is arranged in the sampling tube, and the sampling rod passes through the retaining ring. The first retaining piece on the sampling rod abuts against the front side surface of the retaining ring. The rear end of the sampling rod is installed with a second retaining piece. A telescopic spring is sleeved on the sampling rod, and the two ends of the telescopic spring respectively abut against the retaining ring and the second retaining piece. A plurality of scrapers at different positions are arranged on the outer wall of the front half of the sampling rod. A push rod is connected to the rear end of the sampling rod. The end of the push rod extends out of the sampling tube and is provided with a push plate. The push plate can be held to push the push rod forward, and the sampling rod extends out of the sampling tube and extends into the anus and intestines of the livestock. The scraper on the front half of the sampling rod can be used to scrape feces. The push plate is released, and the sampling rod is separated from the anus and intestines of the livestock to complete feces sampling. The feces sampling work is relatively convenient and quick.

[0005] The device only has a sampling function. After using the device to take out feces, the user needs to manually load the taken feces sample into the sample transfer container. During this period, the sample will not only be exposed to the outside and may be contaminated, causing detection errors, but the feces itself will be exposed to the outside world during the transfer process, which will also cause environmental pollution and may cause discomfort to the transfer operator. Summary of the invention

[0006] In order to solve the problems mentioned in the above background technology, the present invention provides a sample collection device for coccidia oocyst detection and a sample detection method.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] A sample collection device for coccidia oocyst detection comprises a sampling rod, a collecting tube, a rotating bin and a transfer assembly, wherein at least one collecting groove is arranged on the peripheral wall of the lower end of the sampling rod, a recovery groove is arranged on the upper part of the collecting groove, a closed side plate which can move up and down is arranged in the recovery groove, a storage channel is arranged at a position of the lower end of the sampling rod corresponding to the collecting groove, a bottom sealing block is arranged in the storage channel, a blocking block which can move up and down is arranged between the storage channel and the collecting groove, the bottom sealing block can be driven by a translation driving rod to translate and be detachably connected with the closed side plate, and the transfer assembly can move in a transport cavity which is arranged above the collecting groove and communicates with the collecting groove, so as to plug the open mouth at the bottom end of the transfer cylinder into the communicating channel between the collecting groove and the transport cavity;

[0009] The rotating chamber includes two upper and lower rotating circles, which can rotate with the central axis of the sampling rod as the rotation axis. There are multiple placement cavities arranged in a circular array in the rotating circle. A connecting window is provided at a position on the side wall of the sampling rod corresponding to the rotating chamber. The placement cavity has an open channel extending toward the side wall of the sampling rod. There is a gap between the upper and lower rotating circles. A telescopic clamp is installed on the outside of the sampling rod. The telescopic clamp can be extended to pass through the gap between the rotating circles and probe into the placement cavity to grab the collection tube and move it from the placement cavity through the connecting window into the sampling rod.

[0010] Preferably, when the closed side plate extends downward into the collecting tank, it has a spacing from the inner side wall of the collecting tank, so that after the feces fills the collecting tank, the closed side plate moved into the collecting tank can cut off the feces in the collecting tank from the outside, so that the feces retained in the collecting tank can be sampled and extracted.

[0011] Preferably, the height difference between the blocking block and the storage channel is just equal to the thickness of the bottom sealing block, so that when the blocking block is lifted or lowered to the top or bottom of the storage channel by the lifting motor, the bottom sealing block can just pass through the gap between the blocking block and the bottom or top of the storage channel and enter the collecting tank. The bottom sealing block includes a main block body and a docking joint arranged toward the collecting tank. The horizontal cross-section of the main block body can just fill the gap between the closed side panel and the collecting tank. The bottom of the closed side panel has a docking port for allowing the docking joint to be inserted, and the docking joint can be detachably connected to the docking port.

[0012] Preferably, the translation drive rod is installed at the upper and lower ends of the storage channel, and the end of the translation drive rod is installed with an electromagnetic docking block. The side of the main block body facing the translation drive rod has a groove that can just dock with the electromagnetic docking block. A permanent magnet is installed in the groove of the main block body. After the electromagnetic docking block is energized to generate strong magnetism, it can be connected to the bottom sealing block with a sufficiently strong magnetic attraction force, so that the translation drive rod can drive the bottom sealing block to separate from the mating closed side plate, or overcome the resistance to push the bottom sealing block and insert the docking head into the docking port so that the bottom sealing block is matingly connected to the closed side plate.

[0013] Preferably, the transfer assembly includes a transfer cylinder, a telescopic frame and a vertical shift rod. The vertical shift rod is controlled by a controller and can be hydraulically extended and retracted. The telescopic frame is installed on the outer periphery of the sub-control head at the lower end of the vertical shift rod. The transfer cylinder is installed on the end of the telescopic frame away from the sub-control head. The transfer cylinder is hollow inside and open at the bottom. A sealing cover is installed at the bottom of the transfer cylinder. The sealing cover is connected to the bottom edge of the transfer cylinder by an electric hinge to control the opening and closing of the bottom end of the transfer cylinder.

[0014] Preferably, there is a transfer channel extending to the setting position of the rotary bin above the transfer chamber, and an end motor is installed on the top of the transfer cylinder. The end motor is used to drive the axial rotation of the screw in the transfer cylinder, thereby driving the discharge push block set in the transfer cylinder to move up and down. The outer peripheral wall of the discharge push block is in close contact with the inner wall of the transfer cylinder, so that when the discharge push block moves downward, the fecal sample in the transfer cylinder can be completely pushed out from the open port at the bottom of the transfer cylinder.

[0015] Preferably, the rotating ring is detachably mounted with a shielding plate for closing the opening of the placement cavity, so that after the collecting tube is placed in the placement cavity, the user can keep the collecting tube in the placement cavity by installing the shielding plate.

[0016] A method for detecting a sample of coccidia oocysts is also provided, comprising the following steps:

[0017] S1: inserting one end of the sampling rod having the collecting groove into a pile of fresh livestock feces or the rectum of livestock to obtain a feces sample;

[0018] S2: Take out the collection tube containing the feces sample from the rotating bin, add saturated urea solution into the collection tube, so that the ratio of feces to saturated urea solution in the collection tube is 1:10-1:20, and then fully stir the feces and saturated urea solution to make the feces completely dispersed in the saturated urea solution to form a uniform suspension;

[0019] S3: Place the collection tube containing the mixed suspension into a centrifuge, set the centrifugal speed of the centrifuge to 1500-2000 rpm, and set the centrifugal time to 5-10 minutes. After the centrifugation is completed, the coccidia oocysts will float to the surface of the liquid;

[0020] S4: Use a pipette or a pipette to carefully absorb a layer of the liquid surface and transfer it to a glass slide. Then add an appropriate amount of mounting medium to the slide and gently cover it with a coverslip to avoid bubbles.

[0021] S5: Place the prepared slide under a microscope for observation to detect information such as the type and quantity of coccidia.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention uses a method of mixing saturated urea with fecal samples to process samples. The specific gravity of coccidia oocysts is smaller than that of saturated urea solution. After centrifugation and other operations, the oocysts will float to the surface of the liquid. At the same time, urea accelerates protein sedimentation and reduces interfering foreign matter such as fat particles, which is convenient for collection and observation. The present invention is a method for enriching oocysts based on the physical properties and density differences of coccidia, which can increase the sensitivity of detection and make it easier to find coccidia oocysts.

[0024] 2. The sampling equipment used in the present invention can simultaneously sample feces from the fresh feces pile and rectum of livestock, and can perform multi-scene sampling, with better applicability. In addition, multiple collecting troughs can be provided in the present invention, so that multi-point sampling can be performed in one sampling operation, and sample acquisition is more random, reducing detection errors.

[0025] 3. The entire process from sampling to transferring the fecal sample to the collection tube can be automatically completed in the sampling rod through the cooperation of the rotating bin, the transfer component and the grabbing component, which not only avoids the fecal sample being exposed to the outside world and causing environmental pollution during the transfer process, but also does not require human participation throughout the transfer process and will not cause discomfort to the user. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 This is a schematic structural diagram of a sample collection device for detecting coccidia oocysts according to the present invention;

[0028] Figure 2 This is a schematic diagram of the bottom structure of a sampling rod of a sample collection device for coccidia oocyst detection according to the present invention;

[0029] Figure 3 for Figure 2 The enlarged schematic diagram at A in the middle;

[0030] Figure 4 for Figure 2 The enlarged schematic diagram of point B in the middle;

[0031] Figure 5 This is a schematic diagram of the matching structure of the rotary bin and the sampling rod of the present invention;

[0032] Figure 6 It is a schematic diagram of the coordination structure of the rotary bin and the grasping and moving assembly described in the present invention.

[0033] In the figure: 1. sampling rod; 101. collecting tank; 102. recovery tank; 103. storage channel; 104. docking port; 105. transport chamber; 106. transfer channel; 107. anti-slip slide rail; 108. connecting window; 11. closed side panel; 12. lifting motor; 13. interactive device; 14. controller; 15. bottom sealing block; 151. main block; 152. docking port; 16. barrier block; 16. barrier block; 17. hoisting motor; 18 , translation drive rod; 19, electromagnetic docking block; 2, collecting tube; 3, rotating bin; 301, guide ring rail; 302, placement chamber; 31, rotating circle; 32, driving frame rod; 33, driving device; 34, shielding plate; 4, transfer assembly; 41, transfer cylinder; 42, telescopic frame; 43, vertical shift rod; 44, sub-control head; 45, sealing cover; 46, end motor; 47, discharge push block; 5, grabbing assembly; 51, fixing block; 52, telescopic chuck. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Example 1

[0036] Reference Figure 1-6 A sample collection device for coccidia oocyst detection includes a sampling rod 1, a collecting tube 2, a rotating bin 3 and a transfer assembly 4. The rotating bin 3 is installed on the upper part of the sampling rod 1, the collecting tube 2 is removably installed in the rotating bin 3, and the transfer assembly 4 is installed inside the sampling rod 1.

[0037] The sampling rod 1 has a rounded probe at the lower end, and the outer peripheral wall of the probe is provided with at least one collecting groove 101. The upper part of the collecting groove 101 has a recovery groove 102, and a closed side plate 11 is provided in the recovery groove 102. A lifting motor 12 is installed above the recovery groove 102. The lifting motor 12 is connected to the closed side plate 11 through a hydraulic telescopic rod. The lifting motor 12 can control the extension and contraction of the hydraulic telescopic rod to drive the closed side plate 11 to extend downward from the recovery groove 102 and enter the collecting groove 101, or move upward and retract into the recovery groove 102. The closed side plate 11 extends downward into the collecting groove 101, there is a spacing distance between the closed side plate 11 and the inner wall of the collecting tank 101. Before sampling, the closed side plate 11 is moved into the recovery tank 102. After the user inserts the round probe of the sampling rod 1 into the rectum of the livestock or the feces pile, the feces will fill the collecting tank 101. At this time, the user inputs a command to the interactive device 13 installed on the outer peripheral surface of the sampling rod 1, so that the controller 14 controls the lifting motor 12 to drive the closed side plate 11 to extend downward into the collecting tank 101 after receiving the command. At this time, the closed side plate 11 cuts off the feces in the collecting tank 101 from the outside, and part of the feces is retained in the collecting tank 101 as a sample.

[0038] A storage channel 103 is provided at a position corresponding to the collecting tank 101 at the lower end of the sampling rod 1, a bottom sealing block 15 is provided in the storage channel 103, and a blocking block 16 capable of moving up and down is provided between the storage channel 103 and the collecting tank 101, and the height difference between the blocking block 16 and the storage channel 103 is just equal to the thickness of the bottom sealing block 15, so that when the blocking block 16 is lifted or lowered to the top or bottom of the storage channel 103 by the lifting motor 17, the bottom sealing block 15 can just pass through the gap between the blocking block 16 and the bottom or top of the storage channel 103 and enter the collecting tank 101, and when the bottom sealing block 15 is in the gap between the blocking block 16 and the bottom or top of the storage channel 103, the storage channel 103 can also be completely isolated from the collecting tank 101 to prevent feces from entering the storage channel 103 and affecting the up and down movement of the bottom sealing block 15 therein.

[0039] The bottom sealing block 15 includes a main block body 151 and a docking joint 152 arranged toward the collecting groove 101, the horizontal cross-section of the main block body 151 can just fill the gap between the closed side plate 11 and the collecting groove 101, the bottom of the closed side plate 11 has a docking port 104 allowing the docking joint 152 to be inserted, the docking joint 152 and the docking port 104 are detachably connected through a matching structure such as a groove and a spring contact, the upper and lower ends of the storage channel 103 are respectively installed with a translation drive rod 18, the end of the translation drive rod 18 is installed with an electromagnetic docking block 19, the main block body 151 has a groove on the side facing the translation drive rod 18 that can just dock with the electromagnetic docking block 19, a permanent magnet is installed in the groove of the main block body 151, and the electromagnetic docking block 19 can dock with the groove of the main block body 151 after the translation drive rod 18 is extended.

[0040] After the electromagnetic docking block 19 is energized to generate strong magnetism, the translation drive rod 18 with the electromagnetic docking block 19 installed at the end is magnetically connected to the bottom sealing block 15, so that the extension and retraction of the translation drive rod 18 can drive the bottom sealing block 15 to move horizontally. Since the magnetic connection between the bottom sealing block 15 and the electromagnetic docking block 19 is tight enough, the translation drive rod 18 can drive the bottom sealing block 15 to disengage from the matched closed side plate 11, or overcome the resistance to push the bottom sealing block 15 and insert the docking head 152 into the docking port 104 to make the bottom sealing block 15 and the closed side plate 11 matched and connected. The electromagnetic docking block 19 can also be powered off to release the magnetic connection with the bottom sealing block 15, so that the translation drive rod 18 is shortened and the electromagnetic docking block 19 is separated from the bottom sealing block 15 while keeping the bottom sealing block 15 connected to the closed side plate 11, so that the closed side plate 11 can drive the closed side plate 11 to move vertically in the collecting tank 101.

[0041] A transfer chamber 105 connected to the collecting tank 101 is provided above the collecting tank 101. The transfer assembly 4 includes a transfer cylinder 41, a telescopic frame 42 and a vertical shift rod 43. The vertical shift rod 43 is regulated by the controller 14 and can be hydraulically extended and retracted. The telescopic frame 42 is installed on the outer periphery of the sub-control head 44 at the lower end of the vertical shift rod 43. The transfer cylinder 41 is installed on the end of the telescopic frame 42 away from the sub-control head 44. The transfer cylinder 41 is hollow inside and open at the bottom. A sealing cover 45 is installed at the bottom of the transfer cylinder 41. The sealing cover 45 is connected to the bottom edge of the transfer cylinder 41 by an electric hinge to control the opening and closing of the bottom end of the transfer cylinder 41.

[0042] When sampling feces, the transfer cylinder 41 is inserted into the connecting channel between the collecting tank 101 and the transfer chamber 105 in a bottom-sealed state to seal the upper part of the collecting tank 101. After the closed side plate 11 moves down to block the feces in the collecting tank 101 from the outside, the bottom sealing block 15 at the bottom of the storage channel 103 is pushed out by the translation drive rod 18 and connected to the closed side plate 11. At this time, the feces sample on the top of the bottom sealing block 15 will be blocked from the feces at the bottom. Then the transfer cylinder 41 is lifted a little distance to leave space for the sealing cover 45 to unfold. After the transfer cylinder 41 is at the lower end, it is re-docked with the collecting tank 101. Finally, the lifting motor 12 pulls the closed side plate 11 and the bottom sealing block 15 connected thereto toward the transfer cylinder 41, thereby squeezing the feces sample in the collecting tank 101 into the transfer cylinder 41. After the feces sample is squeezed into the transfer cylinder 41, the sealing cover 45 is driven by the electric hinge to keep the lower end of the transfer cylinder 41 closed.

[0043] After the bottom sealing block 15 moves to the top of the collecting tank 101 along with the closed side plate 11, the blocking block 16, which was originally lifted by the lifting motor 17 and tightly attached to the top surface of the storage channel 103, is lowered, thereby opening a space at the top of the storage channel 103 to allow the bottom sealing block 15 to move into the storage channel 103. At this time, the translation drive rod 18 arranged at the top of the storage channel 103 is extended and the electromagnetic docking block 19 at its end is magnetically connected to the bottom sealing block 15, thereby pulling the bottom sealing block 15 away from the closed side plate 11, and moving back into the storage channel 103 as the translation drive rod 18 retracts. The side walls of the storage channel 103 limit the bottom sealing block 15 to move vertically therein but cannot rotate or flip. After the bottom sealing block 15 is moved back to the storage channel 103, the blocking block 16 moves up again, leaving gaps at the upper and lower ends of the blocking block 16 that prevent the bottom sealing block 15 from moving out, so that the bottom sealing block 15 can be stored in the storage channel 103 until the next sampling.

[0044] Example 2

[0045] Reference Figure 1-6The difference between this embodiment and embodiment 1 is that a transfer channel 106 extending to the setting position of the rotary bin 3 is provided above the transfer chamber 105. After receiving the fecal sample, the transfer cylinder 41 will move into the transfer chamber 105 together with the telescopic frame 42 due to the shortening of the vertical moving rod 43. Then, the sub-control head 44 controls the shortening of the telescopic frame 42 to move the transfer cylinder 41 toward the central axis of the sampling rod 1, so that the transfer cylinder 41 can continue to enter the transfer channel 106 as the vertical moving rod 43 shortens. An end motor 46 is installed on the top of the transfer cylinder 41. The end motor 46 is used to drive the axial rotation of the screw in the transfer cylinder 41, thereby driving the discharge push block 47 set in the transfer cylinder 41 to move up and down. The outer peripheral wall of the discharge push block 47 is in close contact with the inner wall of the transfer cylinder 41, so that when the discharge push block 47 moves downward, the fecal sample in the transfer cylinder 41 can be completely pushed out from the open mouth at the bottom of the transfer cylinder 41.

[0046] The rotary bin 3 includes two upper and lower rotary circles 31, and the rotary circle 31 is maintained at a specified vertical position by a driving frame rod 32 installed on the outside of the sampling rod 1. One end of the driving frame rod 32 is fixedly installed on the sampling rod 1, and the other end is equipped with a driving device 33 that cooperates with the guide ring rail 301 of the rotary circle 31. The guide ring rail 301 is annularly arranged on the rotary circle 31. The driving device 33 can drive the rotating wheel whose track is clamped on the guide ring rail 301 to rotate, thereby driving the rotary circle 31 to rotate with the central axis of the sampling rod 1 as the rotation axis.

[0047] The rotating circle 31 has a plurality of placement cavities 302 arranged in a circular array, and each placement cavity 302 passes through the rotating circle 31 from top to bottom so that the collecting tube 2 can be placed therein and rotate around the sampling rod 1 with the rotating circle 31. The upper surface of the upper rotating circle 31 and the lower surface of the lower rotating circle 31 are removably provided with a shielding plate 34 for closing the opening of the placement cavity 302. Before sampling, the user can place the collecting tube 2 in the placement cavity 302 by removing the shielding plate 34 provided on the upper rotating circle 31. After the collecting tube 2 is placed in the placement cavity 302, the user can keep the collecting tube 2 in the placement cavity 302 by installing the shielding plate 34. After the sampling is completed, the user can remove the shielding plate 34 provided on the lower rotating circle 31 to take the collecting tube 2 out of the placement cavity 302.

[0048] Preferably, the outer wall of the sampling rod 1 is provided with an anti-slip rail 107, and the edge of the shielding plate 34 close to the outer wall of the sampling rod 1 is provided with a block engaged with the anti-slip rail 107, so that after the shielding plate 34 is removed and separated from the rotating circle 31, it can move vertically along the anti-slip rail 107 to free up space for the collecting tube 2 to be placed in and removed from the placement cavity 302, and always maintain connection with the sampling rod 1 without being detached and lost to cause inconvenience.

[0049] A connecting window 108 is provided at a position on the side wall of the sampling rod 1 corresponding to the rotating bin 3, and the placement cavity 302 has an open channel extending toward the side wall of the sampling rod 1, so that the collection tube 2 in the placement cavity 302 can pass through the connecting window 108 and enter the transfer channel 106 when the placement cavity 302 where the collection tube 2 is located moves to correspond to the connecting window 108.

[0050] A grasping and moving assembly 5 is installed at a position on the outer peripheral wall of the sampling rod 1 corresponding to the connecting window 108, and the grasping and moving assembly 5 includes a fixed block 51 fixedly installed on the outer peripheral wall of the sampling rod 1, and the fixed block 51 has a space for allowing the rotating circle 31 to pass through on the side facing the sampling rod 1, and there is a gap between the upper and lower rotating circles 31. The fixed block 51 is installed with a telescopic clamp 52 at a position corresponding to the gap between the two rotating circles 31, one end of the telescopic clamp 52 is fixedly installed on the fixed block 51, and the other end can be extended to pass through the gap between the two rotating circles 31 and probe into the placement cavity 302. A mechanical clamp is installed at one end of the telescopic clamp 52 that can probe into the placement cavity 302, and the mechanical clamp can clamp the collection tube 2 after probing into the placement cavity 302, thereby moving the collection tube 2 from the placement cavity 302 through the connecting window 108 into the transfer channel 106.

[0051] After the stool sample is loaded, the transfer cylinder 41 moves upward as the vertical moving rod 43 shortens until it is above the rotary bin 3. When the telescopic clamp 52 grabs the collecting tube 2 and moves to the bottom of the transfer cylinder 41, the discharge push block 47 can squeeze the stool sample downward and be received by the collecting tube 2. The collecting tube 2 that has received the stool sample is moved back to the placement chamber 302 by the telescopic clamp 52. After the collection tube 2 loaded with the stool sample is moved back to the placement chamber 302, it rotates with the rotating circle 31 so that an adjacent collection tube 2 that has not received the stool sample is moved to a position corresponding to the connecting window 108. After the transfer assembly 4 obtains the next batch of stool samples and moves the transfer cylinder 41 above the rotary bin 3, the collection tube 2 that has not received the stool sample can be moved into the transfer channel 106 by the telescopic clamp 52 to receive the newly obtained stool sample. By analogy, all collection tubes 2 can be loaded with stool samples according to the aforementioned steps.

[0052] Example 3

[0053] A method for detecting a sample of coccidia oocysts comprises the following steps:

[0054] S1: inserting one end of the sampling rod 1 having the collecting groove 101 into a pile of fresh livestock feces or the rectum of livestock to obtain a feces sample;

[0055] S2: taking out the collection tube 2 containing the feces sample from the rotating bin 3, adding saturated urea solution into the collection tube 2, so that the ratio of feces to saturated urea solution in the collection tube 2 is 1:10-1:20, and then fully stirring the feces and saturated urea solution, so that the feces are completely dispersed in the saturated urea solution to form a uniform suspension;

[0056] S3: placing the collection tube 2 containing the mixed suspension into a centrifuge, setting the centrifugal speed of the centrifuge to 1500-2000 rpm, and setting the centrifugal time to 5-10 minutes. After the centrifugation is completed, the coccidia oocysts will float to the surface of the liquid;

[0057] S4: Use a pipette or a pipette to carefully suck out a layer of liquid on the surface (containing the floating oocysts) and transfer it to a glass slide. Then add an appropriate amount of mounting medium (such as glycerol) on the slide and gently cover it with a coverslip to avoid bubbles.

[0058] S5: Place the prepared slide under a microscope for observation to detect information such as the type and quantity of coccidia.

[0059] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0060] In the present invention, unless otherwise clearly specified and limited, the terms "set", "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0061] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0062] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A sample collection device for coccidia oocyst detection, comprising a sampling rod (1), a collection tube (2), a rotating bin (3) and a transfer assembly (4), characterized in that: The sampling rod (1) is provided with at least one collecting groove (101) on the peripheral wall at the lower end thereof, and a recovery groove (102) is provided on the upper part of the collecting groove (101), and a closed side plate (11) capable of moving up and down is provided in the recovery groove (102); a storage channel (103) is provided at a position of the lower end of the sampling rod (1) corresponding to the collecting groove (101), and a bottom sealing block (15) is provided in the storage channel (103); a blocking block (16) capable of moving up and down is provided between the storage channel (103) and the collecting groove (101), and the bottom sealing block (15) can be driven by a translation driving rod (18) to translate and be detachably connected to the closed side plate (11); the transfer assembly (4) can move in a transfer cavity (105) provided above the collecting groove (101) and communicating therewith, so as to insert the open opening at the bottom end of the transfer cylinder (41) into the communicating channel between the collecting groove (101) and the transfer cavity (105); The rotary chamber (3) comprises two upper and lower rotary circles (31), the rotary circles (31) being capable of rotating with the central axis of the sampling rod (1) as the rotation axis, the rotary circles (31) comprising a plurality of placement cavities (302) arranged in a circumferential array, a connecting window (108) being provided at a position of the side wall of the sampling rod (1) corresponding to the rotary chamber (3), the placement cavity (302) comprising a channel extending and opening toward the side wall of the sampling rod (1), a gap being provided between the upper and lower parts of the rotary circles (31), a telescopic chuck (52) being mounted on the outside of the sampling rod (1), the telescopic chuck (52) being capable of extending and passing through the gap between the rotary circles (31) and probing into the placement cavity (302), so as to grasp the collection tube 2 and move it from the placement cavity (302) through the connecting window (108) into the sampling rod (1).

2. A sample collection device for coccidia oocyst detection according to claim 1, characterized in that: When the closed side plate (11) extends downward into the collecting tank (101), there is a distance between the closed side plate (11) and the inner wall of the collecting tank (101), so that after the feces fills the collecting tank (101), the closed side plate (11) moved into the collecting tank (101) can cut off the feces in the collecting tank (101) from the outside, so that the feces retained in the collecting tank (101) can be sampled and extracted.

3. A sample collection device for coccidia oocyst detection according to claim 1, characterized in that: The height difference between the blocking block (16) and the storage channel (103) is just equal to the thickness of the bottom sealing block (15), so that when the blocking block (16) is lifted or lowered to the top or bottom of the storage channel (103) by the lifting motor (17), the bottom sealing block (15) can just pass through the gap between the blocking block (16) and the bottom or top of the storage channel (103) and enter the collection tank (101). The bottom sealing block (15) includes a main block body (151) and a docking head (152) arranged toward the collection tank (101), and the horizontal cross-section of the main block body (151) can just fill the gap between the closed side plate (11) and the collection tank (101). The bottom of the closed side plate (11) has a docking port (104) for allowing the docking head (152) to be inserted, and the docking head (152) can be detachably connected to the docking port (104).

4. A sample collection device for coccidia oocyst detection according to claim 3, characterized in that: The translation driving rod (18) is installed at the upper and lower ends of the storage channel (103), and the end of the translation driving rod (18) is installed with an electromagnetic docking block (19). The main block (151) has a groove on the side facing the translation driving rod (18) that can just dock with the electromagnetic docking block (19), and a permanent magnet is installed in the groove of the main block (151). After the electromagnetic docking block (19) is energized to generate strong magnetism, it can be connected to the bottom sealing block (15) with a sufficiently strong magnetic attraction force, so that the translation driving rod (18) can drive the bottom sealing block (15) to separate from the matching closed side plate (11), or overcome the resistance to push the bottom sealing block (15) and insert the docking head (152) into the docking port (104) so ​​that the bottom sealing block (15) is matched and connected with the closed side plate (11).

5. A sample collection device for coccidia oocyst detection according to claim 1, characterized in that: The transfer assembly (4) comprises a transfer cylinder (41), a telescopic frame (42) and a vertical shift rod (43); the vertical shift rod (43) is controlled by a controller (14) and can be hydraulically extended and retracted; the telescopic frame (42) is mounted on the outer periphery of a sub-control head (44) at the lower end of the vertical shift rod (43); the transfer cylinder (41) is mounted on one end of the telescopic frame (42) away from the sub-control head (44); the transfer cylinder (41) is hollow inside and open at the bottom; a sealing cover (45) is installed at the bottom of the transfer cylinder (41); the sealing cover (45) is connected to the bottom edge of the transfer cylinder (41) by an electric hinge to control the opening and closing of the bottom end of the transfer cylinder (41).

6. A sample collection device for coccidia oocyst detection according to claim 5, characterized in that: A transfer passage (106) extending to the location where the rotary bin (3) is set is provided above the transfer chamber (105). A terminal motor (46) is installed at the top of the transfer cylinder (41). The terminal motor (46) is used to drive the screw in the transfer cylinder (41) to rotate axially, thereby driving the discharge push block (47) provided in the transfer cylinder (41) to move up and down. The outer peripheral wall of the discharge push block (47) is in close contact with the inner wall of the transfer cylinder (41), so that when the discharge push block (47) moves downward, the fecal sample in the transfer cylinder (41) can be completely pushed out from the open opening at the bottom of the transfer cylinder (41).

7. A sample collection device for coccidia oocyst detection according to claim 5, characterized in that: The rotating ring (31) is detachably mounted with a shielding plate (34) for closing the opening of the placement cavity (302), so that after the collection tube (2) is placed in the placement cavity (302), the user can retain the collection tube (2) in the placement cavity (302) by installing the shielding plate (34).

8. A method for detecting a sample of coccidia oocysts, using the sample collection device for detecting coccidia oocysts as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: S1: inserting one end of the sampling rod (1) having the collecting groove (101) into a pile of fresh livestock feces or the rectum of livestock to obtain a feces sample; S2: taking out the collection tube (2) containing the feces sample from the rotating bin (3), adding saturated urea solution into the collection tube (2) so that the ratio of feces to saturated urea solution in the collection tube (2) is 1:10-1:20, and then fully stirring the feces and saturated urea solution so that the feces are completely dispersed in the saturated urea solution to form a uniform suspension; S3: placing the collection tube (2) containing the mixed suspension into a centrifuge, setting the centrifugal speed of the centrifuge to 1500-2000 rpm, and setting the centrifugal time to 5-10 minutes. After the centrifugation is completed, the coccidia oocysts will float to the surface of the liquid; S4: Use a pipette or a pipette to carefully absorb a layer of the liquid surface and transfer it to a glass slide. Then add an appropriate amount of mounting medium to the slide and gently cover it with a coverslip to avoid bubbles. S5: Place the prepared slide under a microscope for observation to detect information such as the type and quantity of coccidia.

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