Special multi-dimensional spiral positioning layer sowing inoculator for edible mushroom liquid strain

By designing a multi-dimensional spiral positioning and layer seeding inoculation device, the multi-dimensional inoculation of edible fungi liquid strains in the bacterial package base material is solved, and the slow colonization and aging problems caused by single-sided inoculation properties in the existing technology are improved, and the mycelium development speed and anti-miscellaneous ability are improved.

CN119744714APending Publication Date: 2025-04-04JIANGSU TONGYANG EQUIPMENT TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510029170.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-08
Publication Date
2025-04-04

AI Technical Summary

Technical Problem

The existing edible fungi liquid bacterial inoculation methods have single-sided inoculation properties, which leads to slow mycelium colonization and feeding, and liquid bacterial strains are prone to aging after they are bred, resulting in an increase in the risk of infection.

Method used

A special multi-dimensional spiral-shaped positioning layer-sowing inoculation device for liquid edible fungi is designed. Through the infusion structure and multi-dimensional inoculation structure, the multi-dimensional multi-layer inoculation of the bacterial seeds are realized inoculation of the bacterial seeds in the bacterial package base material to form a uniform spiral inoculation line.

Benefits of technology

The comprehensive contact and seeding between the strain and the base material in the bacteria package is achieved, the mycelium development speed is improved, the cultivation time is shortened, the risk of contamination of miscellaneous bacteria is reduced, and the bacteria are young.

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Abstract

The invention discloses a special multi-dimensional spiral positioning layer sowing inoculator for edible fungus liquid strains, and relates to the technical field of edible fungus equipment.The special multi-dimensional spiral positioning layer sowing inoculator is characterized in that a fungus conveying channel is arranged in a fungus conveying shaft of a fungus conveying structure, an inoculation body of a multi-dimensional inoculation structure is located at one end of the fungus conveying shaft, and a lateral body is arranged on the outer side of the inoculation body and is in a spiral shape; the inoculation body is provided with at least one first fungus conveying hole, the lateral body is provided with at least two second fungus conveying holes, the fungus conveying channels are communicated with the first fungus conveying holes and the second fungus conveying holes respectively, and the first fungus conveying holes are used for achieving inoculation of base materials on the inner surfaces of fungus bag holes or base materials at the axis positions of fungus bags. The second fungus conveying holes are used for inoculating the base materials with different distances from the inner surfaces or the axes of the fungus bag holes to the outer surfaces of the fungus bags. According to the invention, multi-dimensional and multi-layer inoculation of strains in the base material of the fungus bag can be realized, and specific uniform spiral inoculation lines are presented in the base material in the fungus bag.
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Description

Technical Field

[0001] The invention relates to the technical field of edible fungus equipment, and in particular to a multi-dimensional spiral positioning layer seeding inoculator specially used for edible fungus liquid strains. Background Art

[0002] There are two existing methods for inoculating liquid spawn in edible fungi bags: raw material inoculation method and cooked material inoculation method.

[0003] The raw material inoculation method is to pack the raw material into bags first and then sterilize it. There are mainly the following raw material inoculation methods: 1. The plug-in type, that is, there is a through hole in the middle of the mushroom bag for ventilation, such as black fungus bags and other varieties of mushroom bags; 2. The non-plug-in type, that is, there is no ventilation hole in the middle of the mushroom bag, and the middle of the base material is solid, such as shiitake mushrooms; 3. The ring type, that is, after the mushroom bag is packed, the upper and lower through holes are left in the middle, and the plastic cloth at the open end is not tightened, and a ring is added to seal it for ventilation. The plug-in inoculation method, taking the liquid inoculation of black fungus bags as an example, after the mushroom bag is taken out of the pot and cooled, the plastic stick inserted in the base material of the mushroom bag is pulled out during inoculation, and then the liquid strain is sprayed on the upper and lower holes of the base material wall of the mushroom bag, and the mycelium germinates and settles to eat the material. The mycelium will produce longitudinally toward the bag wall. Generally, the radius of the black fungus bag is 0.4cm, and it takes an average of 25 days for the mycelium to fill the bag. The non-insertion-rod long-rod inoculation method can only be done by punching holes on the bag surface and then inserting liquid or solid strains on the surface. Solid strains are cultured directly, and liquid strains must be cultured with a breathable patch after insertion. The mycelium has a long journey and slow production. For example, it usually takes 45-60 days for a mushroom bag to grow fully. The ring-type inoculation method generally has a hole in the middle of the base material, and there is also a cross-section on the top surface of the base material for simultaneous inoculation. The feeding speed is not fast, but the air permeability is slightly better, which is not a big advantage, and the upper mycelium is also prone to aging. When using the above three inoculation methods for liquid strain inoculation, they all have one thing in common, that is, they all have single-sided inoculation properties.

[0004] The existing method of inoculating raw materials is to sterilize the raw materials first and then bag them. This inoculation method has made an overall process improvement on the traditional raw material inoculation method. When bagging the raw materials, the fungus can be connected to the center of the bag and the surface of the bag simultaneously (currently it is only used in the production of mushroom bags). It basically achieves the effect of double-sided inoculation, and the overall mycelium colonization and material eating speed is faster. The mycelium colonization and material eating speed are about 50% higher than the traditional raw material cultivation speed, and the efficiency is greatly increased. The existing method of inoculating raw materials has completed the two-sided inoculation properties with new process characteristics, that is, the center of the bag base material and the bag surface are inoculated on both sides. This inoculation method improves the mycelium colonization and germination speed. However, since the liquid fungus itself is in a process of continuous aging and decay after cultivation, although the activity of the liquid fungus is good, its durability also has its own limitations. In order to ensure the transfer germination effect, the base material in the bag is connected after cultivation. The shorter the interval, the better. After it is connected to the surface of the base material for germination and colonization, it will quickly lose its activity and become an aged body. Although connecting the fungus to the surface of the base material can greatly shorten the culture period of the fungus bag, the process of connecting the liquid fungus, which will inevitably become an aged body, to the surface of the base material in the bag is not scientific in principle. In the later mushroom production process, these aging points are easily infected by mold due to their own low activity, which will cause the entire fungus bag to be infected with mold and become scrapped. Only when the hyphae grow from the inside to the outside can the young state of the mycelium on the surface of the base material in the bag be ensured, and the aging germination points at the internal inoculation points are assisted by the internal hyphae. This is the most basic process principle to ensure the anti-impurity ability and mushroom production ability of the fungus bag surface. From the perspective of technological progress, the existing technology is far from reaching a highly scientific and reasonable advanced level, and a more scientific and high-end solution is needed. Summary of the invention

[0005] The purpose of the present invention is to provide a multi-dimensional spiral positioning layer inoculator specially designed for edible fungus liquid strains, which can realize multi-dimensional and multi-faceted inoculation of strains in the base material of the mushroom bag, and present unique and uniform spiral inoculation lines in the base material inside the mushroom bag.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a multi-dimensional spiral positioning layer seeding inoculator specially used for edible fungi liquid spawn, comprising: a bacteria transport structure and a multi-dimensional inoculation structure, wherein the bacteria transport structure comprises a bacteria transport shaft, wherein a bacteria transport channel is arranged in the bacteria transport shaft, and the multi-dimensional inoculation structure comprises an inoculation body and a lateral body, wherein the inoculation body is located at one end of the bacteria transport shaft, and the lateral body is arranged on the outside of the inoculation body, and the lateral body is spiral-shaped, the inoculation body is provided with at least one first bacteria transport hole, and the lateral body is provided with at least two second bacteria transport holes, and the bacteria transport channel is respectively connected with the first bacteria transport hole and the second bacteria transport hole, wherein the first bacteria transport hole is used for inoculating the substrate on the inner surface of the hole of the fungus bag or the substrate at the axis position of the fungus bag, and the second bacteria transport hole is used for inoculating the substrate at different distances between the inner surface of the hole of the fungus bag or the axis of the fungus bag and the outer surface of the fungus bag.

[0008] Preferably, a spiral blade is disposed on the outside of the bacteria transport shaft.

[0009] Preferably, the spiral blade can be docked with the lateral body to form a spiral structure.

[0010] Preferably, the front end of the inoculation body protrudes beyond the front end of the lateral body; or, the front end of the inoculation body is flush with the front end of the lateral body.

[0011] Preferably, there are at least three second bacteria transport holes, and at least three of the second bacteria transport holes are at different distances from the center line of the multi-dimensional inoculation structure.

[0012] Preferably, the bacteria transport structure and the multi-dimensional inoculation structure are detachably connected, and a sealing element is provided at the connection between the bacteria transport structure and the multi-dimensional inoculation structure.

[0013] Preferably, the bacteria transport shaft is threadably connected to the inoculation body.

[0014] Preferably, it also includes a barrel, which is a hollow barrel with openings at both ends, and is located outside the bacteria transport structure and the multi-dimensional inoculation structure.

[0015] Preferably, there is a gap between the barrel and the bacteria transport structure and the multi-dimensional inoculation structure respectively.

[0016] Preferably, a flange is provided on the barrel.

[0017] Compared with the prior art, the present invention has achieved the following technical effects:

[0018] The present invention realizes inoculation of the substrate on the inner surface of the hole of the fungus bag or the substrate at the axis position of the fungus bag through the first bacteria delivery hole, and realizes inoculation of the substrate at different distances between the inner surface of the hole of the fungus bag or the axis of the fungus bag and the outer surface of the fungus bag through the second bacteria delivery hole. The present invention has a simple structure, and the inoculation point breaks through the limited design of single-sided and double-sided, and realizes the multi-dimensional and comprehensive contact and all-round sowing of the strain and the substrate in the fungus bag during the liquid inoculation process in the edible fungus industry, which can increase the mycelium development speed, shorten the breeding time, and fundamentally eliminate the probability of contamination by foreign bacteria. For the first time, it is realized that the all-round development speed in the strain substrate exceeds the growth speed of foreign bacteria. The present invention can ensure that the mycelium grows from the inside to the outside, ensure the synchronous development of the inner, outer and surface layers of the substrate, and ensure the young state of the bacterial body and mycelium. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 This is an axonometric diagram of the multi-dimensional spiral positioning layer seeding inoculator for edible fungi liquid strains of the present invention;

[0021] Figure 2 It is a cross-sectional view of the multi-dimensional spiral positioning layer seeding inoculator for edible fungi liquid strains of the present invention;

[0022] Figure 3 Axonometric measurement of the multi-dimensional inoculation structure of the present invention Figure 1 ;

[0023] Figure 4 The multi-dimensional inoculation structure of the present invention is shown in FIG. Figure 1 ;

[0024] Figure 5 The multi-dimensional inoculation structure of the present invention is cut away Figure 1 ;

[0025] Figure 6 Axonometric measurement of the multi-dimensional inoculation structure of the present invention Figure 2 ;

[0026] Figure 7 The multi-dimensional inoculation structure of the present invention is shown in FIG. Figure 2 ;

[0027] Figure 8 The multi-dimensional inoculation structure of the present invention is cut away Figure 2 ;

[0028] In the figure: 1- bacteria transport channel, 2-first bacteria transport hole, 3-second bacteria transport hole, 4-bacteria transport axis, 5-spiral blade, 6-inoculation body, 7-lateral body, 8-barrel, 9-flange, 10-multi-dimensional inoculation structure. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The purpose of the present invention is to provide a multi-dimensional spiral positioning layer inoculator specially designed for edible fungus liquid strains, which can realize multi-dimensional and multi-faceted inoculation of strains in the base material of the mushroom bag, and present unique and uniform spiral inoculation lines in the base material inside the mushroom bag.

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] like Figures 1 to 8 As shown, the present embodiment provides a multi-dimensional spiral positioning layer seeding inoculator for liquid spawn of edible fungi, comprising: a bacteria transport structure and a multi-dimensional inoculation structure 10, the bacteria transport structure comprising a bacteria transport shaft 4, a bacteria transport channel 1 is arranged in the bacteria transport shaft 4, the multi-dimensional inoculation structure 10 comprises an inoculation body 6 and a lateral body 7 formed in one piece, the inoculation body 6 is located at one end of the bacteria transport shaft 4, the lateral body 7 is arranged on the outside of the inoculation body 6, the lateral body 7 is spiral, the front end of the inoculation body 6 and / or the side wall of the inoculation body 6 are provided with at least one first bacteria transport hole 2, the side wall and the top of the lateral body 7 are respectively provided with at least one second bacteria transport hole 3, the bacteria transport channel 1 is respectively connected with the first bacteria transport hole 2 and the second bacteria transport hole 3, the first bacteria transport hole 2 is used to inoculate the substrate on the inner surface of the hole of the fungus bag or the substrate at the axis position of the fungus bag, and the second bacteria transport hole 3 is used to inoculate the substrate at different distances between the inner surface of the hole of the fungus bag or the axis of the fungus bag to the outer surface of the fungus bag.

[0033] Specifically, in this embodiment, a spiral blade 5 is provided on the outside of the bacteria transport shaft 4, the rotation direction of the lateral body 7 is the same as that of the spiral blade 5, one end of the lateral body 7 can be connected with one end of the spiral blade 5 to form a spiral structure, which can be used to transport the base material during the inoculation process.

[0034] In this embodiment, the diameter of the first bacteria-transmitting holes 2 is about 3.5 mm, and the positions and number of the first bacteria-transmitting holes 2 can be set according to requirements.

[0035] In this embodiment, when the first bacteria input hole 2 is located on the side wall of the inoculation body 6, the first bacteria input hole 2 is arranged close to the front end of the inoculation body 6, and the front end of the inoculation body 6 protrudes from the front end of the lateral body 7. This structure is mostly used for the production of short mushroom bags (the length of the short mushroom bag is generally about 25 cm), such as black fungus bags. The protruding part of the inoculation body 6 is to meet the packaging method of all edible mushroom bags with holes in the middle, and solve the problem of synchronous inoculation of the middle hole during packaging.

[0036] In this embodiment, when the first bacteria delivery hole 2 is located at the front end of the inoculation body 6, the front end of the inoculation body 6 is flush with the front end of the lateral body 7. This structure is used to make a mushroom stick without a hole in the middle, such as a mushroom stick.

[0037] In this embodiment, the second bacteria-transfer hole 3 is arranged near the front end of the lateral body 7. The position and number of the second bacteria-transfer hole 3 can be set according to the needs, and the second bacteria-transfer hole 3 can also be at least three, which is used to inoculate the substrate with different spacings between the inner surface of the hole of the bacteria bag or the axis of the bacteria bag and the outer surface of the bacteria bag.

[0038] In this embodiment, the bacteria transport shaft 4 of the bacteria transport structure and the inoculation body 6 of the multi-dimensional inoculation structure 10 are detachably connected via threads, and a sealing element is provided at the connection between the bacteria transport shaft 4 of the bacteria transport structure and the inoculation body 6 of the multi-dimensional inoculation structure 10, and the sealing element is a rubber pad.

[0039] The present embodiment also includes a barrel 8, which is a hollow barrel with openings at both ends. The barrel 8 is located outside the bacteria transport structure and the multi-dimensional inoculation structure 10. A flange 9 is provided on the barrel 8, and the flange 9 is used to connect to the feed bin of the bagging machine for use with the bagging machine.

[0040] Taking the black fungus bag as an example, the diameter of the bag after packaging is about 10 cm, the diameter of the center hole of the bag is about 2 cm, the distance from the inner wall of the center hole to the surface of the bag is about 4 cm, and the multi-dimensional inoculation structure 10 can be provided with a first bacteria-transfer hole 2 and two second bacteria-transfer holes 3. The first bacteria-transfer hole 2 is located about 2-3 mm near the front end of the inoculation body 6, and one second bacteria-transfer hole 3 is located at the top of the lateral body 7 and is about 4 cm away from the axis of the inoculation body 6. The other second bacteria-transfer hole 3 arranged on the side wall of the lateral body 7 is about 2 cm away from the axis of the inoculation body 6. In actual use, the multi-dimensional inoculation structure 10 with different numbers of first bacteria-transfer holes 2 and second bacteria-transfer holes 3 can be replaced as needed to strengthen the control of the incubation time of the bacteria bag.

[0041] When the multi-dimensional spiral positioning layer seeding inoculator for liquid spawn of edible fungi of the present embodiment is used for inoculation, the initial position of the multi-dimensional inoculation structure 10 is located at the front end of the plastic bag, the bacteria conveying shaft 4 and the multi-dimensional inoculation structure 10 rotate simultaneously, the base material continuously enters the plastic bag, and while the bagging machine is filling the base material, the liquid bacteria are conveyed to the first bacteria conveying hole 2 and the second bacteria conveying hole 3 through the bacteria conveying channel 1 to realize inoculation. As the fungus bag is squeezed and continuously retreats, by controlling the rotation trajectory of the bacteria conveying shaft 4 and the multi-dimensional inoculation structure 10, when the liquid bacteria are inoculated in the base material of the fungus bag, a multi-dimensional, multi-level, multi-ring spiral inoculation trajectory can be formed, which is all-round. The inner, middle and shallow surface of the base material in the bag are covered (the shallow surface is about 2-3mm away from the outer surface), so that the contact surface of the liquid spawn is fully covered, and full-dimensional spawn inoculation is realized, which can greatly compress the mycelium development speed and shorten the spawn bag cultivation time, especially for rare species with a long mycelium development time. The number and density of the first bacteria delivery holes 2 and the second bacteria delivery holes 3 on the multi-dimensional inoculation structure 10 can be adjusted according to the development time of different species, thereby controlling the cultivation time of the spawn bag. Compared with any existing traditional liquid inoculation method, the cultivation time can be reduced. For example, the shiitake mushroom spawn bag is compressed by 50% on the basis of 12 days and the black fungus spawn bag is compressed on the basis of about 8 days.

[0042] This embodiment has a simple structure, and the inoculation points break through the limitations of single-sided and double-sided, so that the bacteria can be inoculated in multiple dimensions and multiple levels in the base material of the bacteria bag, and present unique and uniform spiral inoculation lines in the base material inside the bacteria bag, filling the technical gaps at home and abroad.

[0043] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. Therefore, in summary, the content of this specification should not be understood as a limitation on the present invention.

Claims

1. A multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi, characterized by: include: A bacteria transport structure and a multi-dimensional inoculation structure (10), wherein the bacteria transport structure comprises a bacteria transport axis (4), wherein a bacteria transport channel (1) is arranged inside the bacteria transport axis (4), wherein the multi-dimensional inoculation structure (10) comprises an inoculation body (6) and a lateral body (7), wherein the inoculation body (6) is located at one end of the bacteria transport axis (4), wherein the lateral body (7) is arranged outside the inoculation body (6), wherein the lateral body (7) is spiral-shaped, wherein the inoculation body (6) is provided with at least one first bacteria transport hole (2), wherein the lateral body (7) is provided with at least two second bacteria transport holes (3), wherein the bacteria transport channel (1) is respectively connected with the first bacteria transport hole (2) and the second bacteria transport hole (3), wherein the first bacteria transport hole (2) is used for inoculating the substrate on the inner surface of the hole of the bacteria bag or the substrate at the axis position of the bacteria bag, wherein the second bacteria transport hole (3) is used for inoculating the substrate at different intervals between the inner surface of the hole of the bacteria bag or the axis of the bacteria bag and the outer surface of the bacteria bag.

2. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1 is characterized in that: The exterior of the bacteria transport shaft (4) is provided with a spiral blade (5).

3. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 2 is characterized in that: The spiral blade (5) can be connected with the lateral body (7) to form a spiral structure.

4. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1, characterized in that: The front end of the inoculation body (6) protrudes from the front end of the lateral body (7); or, the front end of the inoculation body (6) is flush with the front end of the lateral body (7).

5. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1, characterized in that: There are at least three second bacteria transport holes (3), and at least three of the second bacteria transport holes (3) are at different distances from the axis of the multi-dimensional inoculation structure (10).

6. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1, characterized in that: The bacteria transport structure and the multi-dimensional inoculation structure (10) are detachably connected, and a sealing element is provided at the connection between the bacteria transport structure and the multi-dimensional inoculation structure (10).

7. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1, characterized in that: The bacteria transport shaft (4) is threadably connected to the inoculation body (6).

8. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 1, characterized in that: It also includes a barrel (8), which is a hollow barrel with openings at both ends. The barrel (8) is located outside the bacteria transport structure and the multi-dimensional inoculation structure (10).

9. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 8, characterized in that: There are gaps between the barrel (8) and the bacteria transport structure and the multi-dimensional inoculation structure (10).

10. The multi-dimensional spiral positioning layer seeding inoculator for liquid edible fungi according to claim 8, characterized in that: The barrel (8) is provided with a flange (9).

Citation Information

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