A composite quaternary ammonium salt disinfection and antibacterial spray and preparation method thereof
By combining natural plant materials with double-stranded quaternary ammonium salts and other substances, a composite quaternary ammonium salt disinfection and antibacterial spray was prepared, which solved the harm of traditional chemical disinfectants to the environment and the human body, as well as the poor effect of natural plant extracts, and achieved efficient and safe disinfection and antibacterial effects.
Patent Information
- Application Number
- CN202411958877.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-04-04
AI Technical Summary
Traditional chemical disinfectants are not easy to degrade in the environment, may have a long-term impact on aquatic organisms and have an irritating effect on the human skin and respiratory tract. Long-term use may lead to drug resistance of bacteria and viruses, reducing the disinfection effect. At the same time, the active ingredients content of natural plant extracts is low, difficult to extract, poor stability, narrow antibacterial spectrum, and is not as significant as that of chemical disinfectants when used alone.
By drying and crushing natural plant materials such as lemongrass, honeysuckle, cloves and sora ginseng, ethanol extraction and enzymatic extraction, the combined extract is mixed with double-stranded quaternary ammonium salt and other substances, and after stirring, emulsifier, polymer and low-molecular stabilizer and other ingredients are added to prepare a composite quaternary ammonium salt disinfection and antibacterial spray.
This spray can reach a logarithmic killing rate of more than 5 in a very short time (within 0.5 minutes), and has efficient disinfection and antibacterial ability to microorganisms such as Staphylococcus aureus, E. coli and Pseudomonas aeruginosa. At the same time, due to its natural ingredients, it is safer to the human body and the environment, reducing its impact on the environment.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of disinfectants, in particular to a composite quaternary ammonium salt disinfection and antibacterial spray and a preparation method thereof. Background Art
[0002] The invention relates to the technical field of disinfectants, in particular to a composite quaternary ammonium salt disinfection and antibacterial spray prepared by utilizing natural plant extracts and quaternary ammonium salt compounds and a preparation method thereof.
[0003] Although traditional chemical disinfectants are effective, they are not easily degraded in the environment and may have long-term effects on aquatic life. Chemical disinfectants are irritating to human skin and respiratory tract and may even cause allergic reactions. Long-term use of chemical disinfectants may cause bacteria and viruses to develop drug resistance and reduce the disinfection effect.
[0004] Natural plant disinfectants have become a research hotspot to replace traditional chemical disinfectants due to their environmental protection, safety and high efficiency. However, the content of active ingredients in natural plants is low and the extraction is difficult. The stability of natural extracts is poor and the antibacterial spectrum of natural plant extracts is narrow. Therefore, they are not as effective as chemical disinfectants when used alone. Summary of the invention
[0005] In view of the above problems, the present invention provides a composite quaternary ammonium salt disinfection and antibacterial spray and a preparation method thereof.
[0006] To achieve the above object, the present invention is implemented by the following technical scheme: a preparation method of a composite quaternary ammonium salt disinfection and antibacterial spray, comprising the following steps:
[0007] S1: Lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder, lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder are mixed to obtain a plant mixed powder, the plant mixed powder is added to 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C. After extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering, the first extraction residue is added to 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C. After extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0008] S2: Mix the second extraction residue in step S1 with deionized water, add complex enzyme and citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain a plant enzymatic extract;
[0009] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, a double-chain quaternary ammonium salt is added to the plant enzymatic extract, stirring is started at a speed of 400 to 600 rpm, stirring for 20 min to 30 min, and ambergris oil, 95% ethanol c, plant extract, emulsifier, polymer stabilizer and low molecular weight stabilizer are continued to be added at a stirring speed of 800 to 1300 rpm, stirring for 30 min to 40 min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0010] Preferably, the weight proportions of the lemongrass powder, honeysuckle pollen, clove powder, sophora flavescens powder, 95% ethanol a, 95% ethanol b, deionized water, complex enzyme, citric acid, plant enzymatic extract, double-chain quaternary ammonium salt, amber oil, 95% ethanol c, plant extract, emulsifier, polymer stabilizer and low-molecular stabilizer are respectively 7-10 parts of lemongrass powder, 7-10 parts of honeysuckle pollen, 5-7 parts of clove powder, 5-7 parts of sophora flavescens powder, 90-110 parts of 95% ethanol a, 50-60 parts of 95% ethanol b, 52-68 parts of deionized water, 3-6 parts of complex enzyme, 2-3 parts of citric acid, 54-70 parts of plant enzymatic extract, 0.5-1 part of double-chain quaternary ammonium salt, 3-5 parts of amber oil, 95% ethanol c. 8-10 parts, plant extract 5-9 parts, emulsifier 0.5-1 part, high molecular weight stabilizer 1-2 parts and low molecular weight stabilizer 0.5-1 part.
[0011] Preferably, the complex enzyme consists of 1-2 parts of pectinase, 1-2 parts of cellulase and 1-2 parts of acid protease by weight.
[0012] Preferably, the polymer stabilizer is composed of 0.2-0.5 parts of polyvinyl alcohol, 0.2-0.5 parts of hydroxypropyl methylcellulose and 0.4-1 parts of polyethylene glycol in parts by weight.
[0013] Preferably, the low molecular weight stabilizer is composed of 0.2-0.5 parts of glycerol and 0.3-1 parts of propylene glycol in parts by weight.
[0014] Preferably, the double-chain quaternary ammonium salt is one or more of didecyldimethylammonium bromide, didecyldimethylammonium chloride, didodecyldimethylammonium chloride, and bis-octyldimethylammonium chloride.
[0015] Preferably, the emulsifier is any one of Tween 20 and Tween 80.
[0016] The present invention provides a composite quaternary ammonium salt disinfection and antibacterial spray and a preparation method thereof. It has the following beneficial effects:
[0017] 1. The composite quaternary ammonium salt disinfection and antibacterial spray prepared by the present invention can effectively kill microorganisms such as Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and achieve a killing rate of more than 5 logarithmic values in a very short time (within 0.5 min), showing efficient disinfection and antibacterial ability.
[0018] 2. The present invention adopts natural plant materials such as lemongrass, honeysuckle, cloves and sophora flavescens, and reduces the use of chemical solvents and the impact on the environment through rotary evaporation extraction and enzymatic extraction, thereby embodying the green and environmentally friendly preparation concept. At the same time, due to its natural ingredients and mild preparation process, the final product is safer to the human body and the environment during use. DETAILED DESCRIPTION
[0019] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0020] Embodiment 1:
[0021] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0022] S1: Lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 9 parts of lemongrass powder, 9 parts of honeysuckle powder, 6 parts of clove powder and 6 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 100 parts of 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C; after extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 55 parts of 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C; after extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0023] S2: Mix the second extraction residue in step S1 with 58 parts of deionized water, add 4.5 parts of complex enzyme (1.5 parts of pectinase, 1.5 parts of cellulase, 1.5 parts of acid protease) and 2 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain the plant enzymatic extract;
[0024] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 62 parts of the plant enzymatic extract and 1 part of the double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400-600rpm, stirring for 20min-30min, and then 4 parts of amber oil, 9 parts of 95% ethanol c, 7 parts of plant extract, 0.75 parts of emulsifier, 1.5 parts of polymer stabilizer (0.4 parts of polyvinyl alcohol, 0.4 parts of hydroxypropyl methylcellulose, 0.7 parts of polyethylene glycol) and 0.75 parts of low molecular weight stabilizer (0.25 parts of glycerol, 0.4 parts of propylene glycol) are added at a stirring speed of 800-1300rpm, stirring for 30min-40min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0025] Embodiment 2:
[0026] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0027] S1: lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 10 parts of lemongrass powder, 10 parts of honeysuckle powder, 7 parts of clove powder and 7 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 110 parts of 95% ethanol a, and a first reflux extraction is performed at 60° C. to 70° C. After extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 60 parts of 95% ethanol b, and a second reflux extraction is performed at 60° C. to 70° C. After extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0028] S2: Mix the second extraction residue in step S1 with 68 parts of deionized water, add 6 parts of complex enzyme (2 parts of pectinase, 2 parts of cellulase, 2 parts of acid protease) and 3 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain the plant enzymatic extract;
[0029] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 70 parts of the plant enzymatic extract and 0.5 parts of a double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400-600 rpm, stirring for 20 min-30 min, and then 5 parts of amber oil, 10 parts of 95% ethanol c, 9 parts of plant extract, 0.5 parts of emulsifier, 2 parts of polymer stabilizer (0.5 parts of polyvinyl alcohol, 0.5 parts of hydroxypropyl methylcellulose, 1 part of polyethylene glycol) and 1 part of low molecular stabilizer (0.5 parts of glycerol, 1 part of propylene glycol) are added at a stirring speed of 800-1300 rpm, stirring for 30 min-40 min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0030] Embodiment 3:
[0031] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0032] S1: lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 7 parts of lemongrass powder, 7 parts of honeysuckle powder, 5 parts of clove powder and 5 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 110 parts of 95% ethanol a, and a first reflux extraction is performed at 60° C. to 70° C. After extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 60 parts of 95% ethanol b, and a second reflux extraction is performed at 60° C. to 70° C. After extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0033] S2: Mix the second extraction residue in step S1 with 52 parts of deionized water, add 3 parts of complex enzyme (1 part of pectinase, 1 part of cellulase, 1 part of acid protease) and 2 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain the plant enzymatic extract;
[0034] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 54 parts of the plant enzymatic extract and 1 part of the double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400-600rpm, stirring for 20min-30min, and then 3 parts of amber oil, 8 parts of 95% ethanol c, 5 parts of plant extract, 0.5 parts of emulsifier, 1 part of polymer stabilizer (0.2 parts of polyvinyl alcohol, 0.2 parts of hydroxypropyl methylcellulose, 0.6 parts of polyethylene glycol) and 0.5 parts of low molecular weight stabilizer (0.2 parts of glycerol, 0.3 parts of propylene glycol) are added at a stirring speed of 800-1300rpm, stirring for 30min-40min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0035] Embodiment 4:
[0036] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0037] S1: Lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powders respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 8 parts of lemongrass powder, 8 parts of honeysuckle powder, 6 parts of clove powder and 6 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 95 parts of 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C; after extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 57 parts of 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C; after extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0038] S2: Mix the second extraction residue in step S1 with 62 parts of deionized water, add 5.4 parts of complex enzyme (1.8 parts of pectinase, 1.8 parts of cellulase, 1.8 parts of acid protease) and 2.4 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain a plant enzymatic extract;
[0039] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 65 parts of the plant enzymatic extract and 0.8 parts of a double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400 to 600 rpm, stirring for 20 min to 30 min, and then 4.5 parts of amber oil, 9.5 parts of 95% ethanol c, 8 parts of plant extract, 0.8 parts of an emulsifier, 1.75 parts of a polymer stabilizer (0.5 parts of polyvinyl alcohol, 0.5 parts of hydroxypropyl methylcellulose, 0.75 parts of polyethylene glycol) and 1 part of a low molecular weight stabilizer (0.4 parts of glycerol, 0.8 parts of propylene glycol) are added at a stirring speed of 800 to 1300 rpm, stirring for 30 min to 40 min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0040] Embodiment 5:
[0041] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0042] S1: Lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 7.5 parts of lemongrass powder, 7.5 parts of honeysuckle powder, 5.5 parts of clove powder and 5.5 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 97.5 parts of 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C; after extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 52.5 parts of 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C; after extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0043] S2: Mix the second extraction residue in step S1 with 56 parts of deionized water, add 3.6 parts of complex enzyme (1.2 parts of pectinase, 1.2 parts of cellulase, 1.2 parts of acid protease) and 2.2 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain a plant enzymatic extract;
[0044] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 57 parts of the plant enzymatic extract and 0.6 parts of the double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400-600 rpm, stirring for 20 min-30 min, and 3.7 parts of amber oil, 8.5 parts of 95% ethanol c, 6 parts of plant extract, 0.6 parts of emulsifier, 1.25 parts of molecular stabilizer (0.4 parts of polyvinyl alcohol, 0.45 parts of hydroxypropyl methylcellulose, 0.4 parts of polyethylene glycol) and 0.75 parts of low molecular stabilizer (0.3 parts of glycerol, 0.45 parts of propylene glycol) are added, the stirring speed is 800-1300 rpm, stirring is carried out for 30 min-40 min, and a composite quaternary ammonium salt disinfection and antibacterial spray is obtained.
[0045] Embodiment 6:
[0046] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0047] S1: lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder; 9.5 parts of lemongrass powder, 9.5 parts of honeysuckle powder, 6.5 parts of clove powder and 6.5 parts of sophora flavescens powder are mixed to obtain a plant mixed powder; the plant mixed powder is added to 102 parts of 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C. After extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering; the first extraction residue is added to 58 parts of 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C. After extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering;
[0048] S2: Mix the second extraction residue in step S1 with 67 parts of deionized water, add 5.7 parts of complex enzyme (1.9 parts of pectinase, 1.9 parts of cellulase, 1.9 parts of acid protease) and 3 parts of citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain a plant enzymatic extract;
[0049] S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, 68 parts of the plant enzymatic extract and 0.9 parts of a double-chain quaternary ammonium salt are mixed, stirring is started at a speed of 400 to 600 rpm, stirring for 20 min to 30 min, and then 4.75 parts of amber oil, 9 parts of 95% ethanol c, 9 parts of plant extract, 0.9 parts of emulsifier, 1.8 parts of polymer stabilizer (0.45 parts of polyvinyl alcohol, 0.45 parts of hydroxypropyl methylcellulose, 0.9 parts of polyethylene glycol) and 1.25 parts of low molecular weight stabilizer (0.45 parts of glycerol, 0.8 parts of propylene glycol) are added at a stirring speed of 800 to 1300 rpm, stirring for 30 min to 40 min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0050] Comparative Example 1:
[0051] In Comparative Example 1, no amber oil, low molecular weight stabilizer and high molecular weight stabilizer were added, and the remaining raw material ratios, preparation methods and operating conditions were the same as those in Example 1.
[0052] Comparative Example 2:
[0053] In Comparative Example 2, no ammonium tar oil was added, and the remaining raw material ratios, preparation methods, and operating conditions were the same as those in Example 1.
[0054] Comparative Example 3:
[0055] In Comparative Example 3, no double-chain quaternary ammonium salt was added, and the remaining raw material ratios, preparation methods, and operating conditions were the same as those in Example 1.
[0056] Comparative Example 4:
[0057] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0058] Mix 62 parts of deionized water and 1 part of double-chain quaternary ammonium salt, start stirring at a speed of 400-600 rpm, stir for 20-30 minutes, continue to add 4 parts of amber oil, 9 parts of 95% ethanol c, emulsifier, 1.5 parts of polymer stabilizer (0.4 parts of polyvinyl alcohol, 0.4 parts of hydroxypropyl methylcellulose, 0.7 parts of polyethylene glycol) and 0.75 parts of low molecular stabilizer (0.25 parts of glycerol, 0.4 parts of propylene glycol), stir at a speed of 800-1300 rpm, stir for 30-40 minutes, and obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0059] Comparative Example 5:
[0060] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0061] Material ratio: 60 parts of deionized water, 3 parts of double-chain quaternary ammonium salt, 4 parts of amber oil, 9 parts of 95% ethanol c, 0.75 parts of emulsifier, 1.5 parts of polymer stabilizer (0.4 parts of polyvinyl alcohol, 0.4 parts of hydroxypropyl methylcellulose, 0.7 parts of polyethylene glycol), 0.75 parts of low molecular weight stabilizer (0.25 parts of glycerol, 0.4 parts of propylene glycol).
[0062] Preparation steps:
[0063] Mix 60 parts of deionized water and 3 parts of double-chain quaternary ammonium salt, start stirring at a speed of 400-600 rpm, stir for 20-30 minutes, continue to add 4 parts of amber oil, 9 parts of 95% ethanol c, emulsifier, 1.5 parts of polymer stabilizer (0.4 parts of polyvinyl alcohol, 0.4 parts of hydroxypropyl methylcellulose, 0.7 parts of polyethylene glycol) and 0.75 parts of low molecular stabilizer (0.25 parts of glycerol, 0.4 parts of propylene glycol), stir at a speed of 800-1300 rpm, stir for 30-40 minutes, and obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0064] Comparative Example 6:
[0065] A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray comprises the following preparation steps:
[0066] Mix 58 parts of deionized water and 5 parts of double-chain quaternary ammonium salt, start stirring at a speed of 400-600 rpm, stir for 20-30 minutes, continue to add 4 parts of amber oil, 9 parts of 95% ethanol c, emulsifier, 1.5 parts of polymer stabilizer (0.4 parts of polyvinyl alcohol, 0.4 parts of hydroxypropyl methylcellulose, 0.7 parts of polyethylene glycol) and 0.75 parts of low molecular stabilizer (0.25 parts of glycerol, 0.4 parts of propylene glycol), stir at a speed of 800-1300 rpm, stir for 30-40 minutes, and obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
[0067] The a, b, and c after the above material names are used for marking only; the materials are essentially the same.
[0068] The composite quaternary ammonium salt disinfection and antibacterial spray prepared in the above Examples 1-6 and Comparative Examples 1-6 was subjected to a microbial killing experiment, referring to the "Technical Specification for Disinfection" 2002 Edition: The suspension method was used to perform killing experiments on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The results of the microbial killing experiment were performed every 0.5 min as shown in Table 1; the composite quaternary ammonium salt disinfection and antibacterial spray prepared in Example 1, Comparative Example 1, Comparative Example 3 and Comparative Example 4 was subjected to quantitative sterilization, and the average killing logarithm of the microorganisms killed after 1 min, 10 min, 30 min and 60 min of Staphylococcus aureus was detected at room temperature of 20±1°C, and the results are shown in Table 2.
[0069] Table 1
[0070]
[0071]
[0072] Table 2
[0073]
[0074] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray, characterized in that: The steps include: S1: Lemongrass, honeysuckle, cloves and sophora flavescens are dried and crushed into coarse powder respectively, and then passed through a 100-mesh sieve to obtain lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder, lemongrass powder, honeysuckle powder, clove powder and sophora flavescens powder are mixed to obtain a plant mixed powder, the plant mixed powder is added to 95% ethanol a, and a first reflux extraction is performed at 60°C to 70°C. After extraction for 3 hours, a first plant ethanol extract and a first extraction residue are obtained by filtering, the first extraction residue is added to 95% ethanol b, and a second reflux extraction is performed at 60°C to 70°C. After extraction for 2 hours, a second plant ethanol extract and a second extraction residue are obtained by filtering; S2: Mix the second extraction residue in step S1 with deionized water, add complex enzyme and citric acid, control the temperature at 40°C to 45°C for enzymatic extraction for 2 to 4 hours, and filter to obtain a plant enzymatic extract; S3: The first plant ethanol extract and the second plant ethanol extract obtained in step S1 are combined and concentrated into a plant extract, a double-chain quaternary ammonium salt is added to the plant enzymatic extract, stirring is started at a speed of 400 to 600 rpm, stirring for 20 min to 30 min, and ambergris oil, 95% ethanol c, plant extract, emulsifier, polymer stabilizer and low molecular weight stabilizer are continued to be added at a stirring speed of 800 to 1300 rpm, stirring for 30 min to 40 min to obtain a composite quaternary ammonium salt disinfection and antibacterial spray.
2. The method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray according to claim 1, wherein: The complex enzyme consists of 1-2 parts of pectinase, 1-2 parts of cellulase and 1-2 parts of acid protease by weight.
3. The method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray according to claim 1, wherein: The polymer stabilizer is composed of 0.2-0.5 parts of polyvinyl alcohol, 0.2-0.5 parts of hydroxypropyl methylcellulose and 0.4-1 parts of polyethylene glycol in parts by weight.
4. The method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray according to claim 1, wherein: The low molecular weight stabilizer is composed of 0.2-0.5 parts of glycerol and 0.3-1 parts of propylene glycol in parts by weight.
5. The method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray according to claim 1, wherein: The double-chain quaternary ammonium salt is one or more of didecyldimethylammonium bromide, didecyldimethylammonium chloride, didodecyldimethylammonium chloride, and didoctyldimethylammonium chloride.
6. The method for preparing a composite quaternary ammonium salt disinfection and antibacterial spray according to claim 1, wherein: The emulsifier is any one of Tween 20 and Tween 80.
7. A composite quaternary ammonium salt disinfection and antibacterial spray, characterized in that: The composite quaternary ammonium salt disinfection and antibacterial spray is obtained by the preparation method of any one of claims 1 to 6.