Botanical attractant for female adult spodoptera frugiperda and preparation method of botanical attractant

Abstract

The invention discloses a plant-derived attractant for female adult spodoptera frugiperda and a preparation method of the plant-derived attractant, and relates to the technical field of preparation of female adult attractants, and the plant-derived attractant comprises the following effective components: 5ng / mu L of leaf acetate, 3ng / mu L of phenethyl acetate and 1ng / mu L of phenylacetaldehyde. Aiming at the problems of high miscellaneous moth trapping rate and monitoring and early warning hysteresis in the field application of the adult spodoptera frugiperda, the invention provides a plant source attractant (lure), the synthesis of the attractant realizes the trapping and killing of female adults and greatly improves the prevention and control effect, and the trapping and killing of one female adult is equivalent to killing of hundreds of larvae at one time.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of female adult insect attractants, and more specifically to a plant-based attractant for female adult insects of fall armyworm and a preparation method thereof. Background Art

[0002] Spodoptera frugiperda (JESmith) (Lepidoptera: Noctuidae), also known as the fall armyworm, is a notorious polyphagous invasive pest native to tropical and subtropical regions of America. The global population of S. frugiperda can be divided into two phenotypically similar but genetically distinct subpopulations or strains: rice strain (RS) and maize strain (CS). The insect feeds on 353 plant species from 76 families.

[0003] At present, chemical pesticides are mainly used to control the fall armyworm. However, the long-term and large-scale use of chemical pesticides has caused the fall armyworm to develop resistance, resulting in low pesticide utilization efficiency, waste of pesticides, and inability to completely control the insects. Insect pheromones are trace volatile chemicals secreted by insects when they reach sexual maturity and can attract individuals of the opposite sex of the same species for mating. They are species-specific and are therefore widely used in the monitoring and control of pests. In recent years, my country has made a series of progress in the industrial synthesis, purification, and stabilization of insect sex pheromones, the improvement and perfection of the slow-release production process, the development and research of various dry traps, the research and development of intelligent sex pheromone detection and reporting technologies, and the trial demonstration of practical sex pheromone technologies for various pests in the field, which has greatly promoted the monitoring, forecasting, and green prevention and control of major pests.

[0004] The monitoring and control area of ​​fall armyworm has a large span, long time and large temperature difference. It is necessary to adopt corresponding attractants for different environments. Different strategies should be adopted in different regions according to its migratory nature, tenderness, sex pheromone release and courtship characteristics. Henan Province is located at 31°23'-36°22' north latitude. It is the main production area of ​​summer corn in the Huanghuaihai area. It is a key prevention area and migration transition area for the world's migratory pest fall armyworm. In field production, the attractants of widely used male adult attractants have a lag in monitoring and early warning (for example, larvae are found earlier in the field in the fall armyworm migration area instead of attracting adults first). Sex attractants mainly attract male adults and have a high rate of mixed moths.

[0005] Therefore, how to provide a plant-based attractant for female adults of the fall armyworm is a technical problem that those skilled in the art urgently need to solve. Summary of the invention

[0006] In view of this, the present invention is proposed.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] The first aspect of the present invention provides a plant-based attractant for female adults of fall armyworm, the active ingredients of which include: 5 ng / μL of phytol acetate, 3 ng / μL of phenylethyl acetate and 1 ng / μL of phenylacetaldehyde. The second aspect of the present invention provides a method for preparing the plant-based attractant for female adults of fall armyworm, the process comprising:

[0009] 1) diluting, respectively diluting leaf acetate, phenylethyl acetate and phenylacetaldehyde to 5 ng / μL, 3 ng / μL and 1 ng / μL with edible oil to obtain leaf acetate solution, phenylethyl acetate solution and phenylacetaldehyde solution;

[0010] 2) mixing, mixing the leaf acetate solution, the phenylethyl acetate solution and the phenylacetaldehyde solution in a volume ratio of 5:3:2 to obtain a mixture; wherein the concentration of each component is 2.5 ng / μL of leaf acetate, 0.9 ng / μL of phenylethyl acetate and 0.2 ng / μL of phenylacetaldehyde.

[0011] 3) Loading: Using a capillary tube as a carrier, the mixture obtained in step 2) is filled into the carrier to obtain a female adult insect plant-derived attractant.

[0012] Preferably, the edible oil includes peanut oil, soybean oil, and corn oil.

[0013] Preferably, in step 3), the amount of the mixture filled into the carrier is 20 mg.

[0014] The third aspect of the present invention provides the use of the plant-derived attractant in attracting female adults of the fall armyworm.

[0015] It can be known from the above technical scheme that the present invention aims at the problems of high rate of attracting other moths and delayed monitoring and early warning in the field application of fall armyworm adult attractant cores, and provides a plant-based attractant (attractant core). The synthesis of the attractant can achieve the trapping and killing of female adults and greatly improve the prevention and control effect. Killing one female adult is equivalent to killing hundreds of larvae at one time. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1

[0018] A plant-derived attractant for female adults of the fall armyworm, wherein active ingredients include 5 ng / μL of phytol acetate, 3 ng / μL of phenylethyl acetate and 1 ng / μL of phenylacetaldehyde.

[0019] A method for preparing a plant-based attractant for female adults of fall armyworm, the process comprising:

[0020] 1) diluting, using edible oil to dilute leaf acetate, phenylethyl acetate and phenylacetaldehyde to 5 ng / μL, 3 ng / μL and 1 ng / μL, respectively, to obtain leaf acetate solution, phenylethyl acetate solution and phenylacetaldehyde solution; the edible oil is peanut oil;

[0021] 2) mixing, mixing the leaf acetate solution, the phenylethyl acetate solution and the phenylacetaldehyde solution in a volume ratio of 5:3:2 to obtain a mixture;

[0022] 3) Loading: Using a capillary tube as a carrier, the mixture obtained in step 2) is filled into the carrier to obtain a female adult insect plant-derived attractant.

[0023] Example 2 Verification of the performance of the above attractant

[0024] A bait validation test was conducted in a maize field (from the tassel stage to the tassel stage) in Baoshan, Yunnan, China (25°08′N, 99°10′E, altitude: 1800 m) from July 28 to September 18, 2024. A single bait was loaded into the top baffle of a bucket device (Beijing Zhongjie Sifang Biotechnology Co., Ltd., Beijing, China). The bucket device was hung on an iron rod 20 cm above the maize seedlings, with an interval of 20 m, and a randomized complete block design was used with 6 replicates. The number of adults captured by each trap was recorded, and the trap was emptied every 2 days. The bucket device without bait was used as a control.

[0025] A total of 188 female adults were trapped in this study, and the difference compared with the control (0 female adults in an empty bucket-type device without a lure) reached an extremely significant level (p<0.01). The increase in the number of female adults lured will lead to a decrease in the mating rate, thereby reducing the reproduction rate and ultimately reducing the degree of damage in the field.

[0026] Example 3 Comparative Test

[0027] A bait validation test was conducted in a maize field (from the tassel stage to the tassel stage) in Baoshan, Yunnan, China (25°08′N, 99°10′E, altitude: 1800 m) from July 28 to September 18, 2024. The baits (leaf acetate only, leaf acetate + phenylethyl acetate mixture, phenylacetaldehyde + leaf acetate + phenylethyl acetate mixture) and the control bait (Zhongjie Sifang) were loaded into the top baffle of a bucket device (Beijing Zhongjie Sifang Biotechnology Co., Ltd., Beijing, China). The bucket device was hung on an iron rod 20 cm above the maize seedlings, with an interval of 20 m, and a randomized complete block design was used with 4 replicates. The number of adults captured by each trap was recorded, and the trap was emptied every 2 days. The Zhongjie Sifang bait was used as a control.

[0028] A total of 186 female adults were trapped in this study, and the difference between the control group with only phytol acetate lure (1 head) and the phytol acetate + phenylethyl acetate mixed lure (3 heads) reached a very significant level (p<0.01). The increase in the number of female adults lured will lead to a decrease in the mating rate, thereby reducing the reproduction rate and ultimately reducing the degree of damage in the field.

[0029] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0030] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A plant-derived attractant for female adults of fall armyworm, characterized in that: The active ingredients include: phytol acetate 5ng / μL, phenylethyl acetate 3ng / μL and phenylacetaldehyde 1ng / μL.

2. The method for preparing the plant-based attractant for female adults of fall armyworm according to claim 1, characterized in that the process include: 1) diluting, respectively diluting leaf acetate, phenylethyl acetate and phenylacetaldehyde to 5 ng / μL, 3 ng / μL and 1 ng / μL with edible oil to obtain leaf acetate solution, phenylethyl acetate solution and phenylacetaldehyde solution; 2) mixing, mixing the leaf acetate solution, the phenylethyl acetate solution and the phenylacetaldehyde solution in a volume ratio of 5:3:2 to obtain a mixture, wherein the concentration of each component is 2.5 ng / μL of leaf acetate, 0.9 ng / μL of phenylethyl acetate and 0.2 ng / μL of phenylacetaldehyde. 3) Loading: Using a capillary tube as a carrier, the mixture obtained in step 2) is filled into the carrier to obtain a female adult insect plant-derived attractant.

3. The method for preparing the plant-derived attractant for female adults of fall armyworm according to claim 2, characterized in that: The edible oil includes peanut oil, soybean oil and corn oil.

4. The method for preparing the plant-based attractant for female adults of fall armyworm according to claim 2, characterized in that: In step 3), the amount of the mixture filled into the carrier is 20 mg.

5. Use of the plant-derived attractant according to claim 1 or the plant-derived attractant prepared by the method according to any one of claims 2 to 4 in attracting female adults of the fall armyworm.