Water disinfection powder containing rose bengal and preparation and application methods thereof

Abstract

The invention discloses rose bengal-containing water disinfection powder as well as a preparation method and an application method of the rose bengal-containing water disinfection powder. The powder is prepared by simply and mechanically mixing rose bengal and citric acid, disinfection can be completed by adding the disinfection powder into a natural water sample and exposing to sunlight for a short period of time (less than or equal to 30 minutes), and the disinfection powder is short in used time, high in efficiency and very suitable for field instant disinfection.

Description

Technical Field

[0001] The invention belongs to the technical field of water treatment, and in particular relates to a water disinfection powder containing Bengal rose red and a preparation and application method thereof. Background Art

[0002] In order to obtain safe drinking water during outdoor travel, it is necessary to disinfect the microorganisms in natural water samples. This method of immediate disinfection at the point of use is called point-of-use water disinfection technology.

[0003] The current mainstream point-of-use disinfection technologies include solar disinfection, chlorination, flocculation and ceramic filtration.

[0004] Ceramic filters are large and inconvenient to carry, while flocculation technology is complicated to handle and has poor results. Therefore, ceramic filtration and flocculation are not suitable for disinfecting water during field trips.

[0005] Chlorination disinfection with hypochlorous acid solution has high efficiency and low cost, and is suitable for outdoor disinfection, but it is often accompanied by unpleasant odor and taste, and disinfection by-products are produced during the disinfection process.

[0006] Solar water disinfection of mineral water bottles has no unpleasant odor or taste, is simple to operate, and is very suitable for outdoor disinfection in sunny places. It is also the cheapest among point-of-use disinfection methods. However, its disinfection efficiency is low, and it usually takes more than 6 hours to complete the disinfection.

[0007] Therefore, it is necessary to develop a solar disinfection enhancement technology to overcome the disadvantage of low efficiency of solar disinfection.

[0008] Bengal Rose Red is a cheap edible pigment used for food coloring. It is highly safe and has no unpleasant odor or taste. It is also a photosensitizer that can produce a large amount of singlet oxygen under the excitation of visible light to inactivate pathogens in water, and self-decomposes under light during disinfection.

[0009] This pigment has been shown to exhibit high inactivation ability against a variety of bacteria and viruses, but its use in water disinfection has been a problem due to its poor inactivation ability against Gram-negative bacteria. Summary of the invention

[0010] The object of the present invention is to provide a water disinfection powder containing Bengal rose red and a preparation and application method thereof, so as to solve the problems raised in the above background technology.

[0011] To achieve the above object, the present invention provides the following technical solution: a water disinfection powder containing Bengal rose red, comprising

[0012] 1:106-265 of Rose Bengal and organic acids.

[0013] Preferably, the photosensitizer is Rose Bengal.

[0014] In any of the above schemes, preferably, the organic acid comprises a combination of one or more of citric acid, tartaric acid and malic acid.

[0015] Preferably, in any of the above schemes, the mass ratio of Bengal rose red to citric acid is 1:106.

[0016] A method for preparing a water disinfection powder containing Bengal rose red comprises the following steps:

[0017] S11: Mix Rose Bengal and organic acid in a certain proportion, wherein the organic acid is a solid acid powder and Rose Bengal is also a fixed powder, and mechanically shake the mixture to obtain a disinfectant powder containing Rose Bengal.

[0018] A disinfectant powder containing Bengal rose red is used in water disinfection, comprising the following steps:

[0019] S21: adding disinfectant powder into the water sample in a certain proportion;

[0020] S22: The water sample is then exposed to sunlight / visible light for disinfection.

[0021] Preferably in any of the above schemes, the water sample is a natural water sample, including surface water, rainwater, etc.

[0022] Preferably, in any of the above schemes, the disinfectant powder is added to the water sample and then dark treated for 0-15 minutes.

[0023] Preferably, in any of the above schemes, the disinfection time is 10-30 minutes.

[0024] Preferably, in any of the above schemes, the mass volume ratio of the disinfectant powder to the water sample is 0.30-0.34 g:1.2 L.

[0025] Preferably, in any of the above schemes, the mass volume ratio of the disinfectant powder to the water sample is 0.32g:1.2L.

[0026] Technical effects and advantages of the present invention:

[0027] 1. The Bengal rose red contained in the powder can be protonated under the action of organic acid, reducing the electrostatic repulsion between the powder and the bacteria and enhancing the adsorption of the Bengal rose red on the bacteria, thereby improving the ability to inactivate Gram-negative bacteria, so that it can be used to enhance sunlight disinfection and overcome the problem of low disinfection efficiency during sunlight disinfection;

[0028] 2. The disinfectant powder prepared by the present application can be added to the natural water sample within 30 minutes to complete the disinfection, which is short in time and high in efficiency;

[0029] 3. The disinfectant powder of the present application has the advantages of being easy to carry, simple to operate, having no unpleasant odor and taste after disinfection, being low cost and safe, etc., and can perfectly meet the disinfection needs during outdoor travel and in areas lacking tap water. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a graph showing the effect of the organic acid Bengal rose red combination on removing Escherichia coli from surface water at pH = 4.25 under visible light;

[0031] Figure 2 This is the removal effect of 0.32g citric acid / Bengal rose red powder on 1.2L surface water of Escherichia coli under sunlight;

[0032] Figure 3 This is a diagram showing the effect of removing E. coli in water in Example 3;

[0033] Figure 4 This is a diagram showing the effect of removing E. coli in water in Example 4;

[0034] Figure 5 Add quantity chart for disinfectant powder;

[0035] Figure 6 The figure is a flow chart of the disinfection of natural water samples by the disinfectant powder of the present invention. DETAILED DESCRIPTION

[0036] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0037] Embodiment 1:

[0038] Material:

[0039] The bacteria used in this example are Escherichia coli DH5α containing kanamycin resistance genes and tetracycline hydrochloride resistance genes, and its resistance to photosensitization disinfection is 7 times that of ordinary Escherichia coli DH5α.

[0040] Bengal rose red was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0041] The visible light source is a Zhongjiao Jinyuan xenon lamp, with an additional filter to provide visible light with a wavelength of ≥420nm and a light intensity of 200mW / cm 2 .

[0042] Collection and use of natural water samples: The water samples were collected from Qizhen Lake in Zijingang Campus of Zhejiang University. The collected water samples were filtered through a 0.22μm mixed cellulose ester water filter membrane to remove the original microorganisms in the water, and stored at -20℃ for use, and restored to room temperature before use.

[0043] Water disinfection process: Surface water was divided into three parts, and an initial concentration of 10 6 cfu / mL of antibiotic-resistant Escherichia coli, followed by the addition of a photosensitizer (Rose Bengal) at a concentration of 2.5 mg / L. Finally, citric acid, tartaric acid, and malic acid were added to three portions respectively to adjust the pH to 4.25. After dark treatment for 15 min, the mixed solution was exposed to visible light, and samples were taken at different time points to analyze the bacterial inactivation.

[0044] The experimental results are as follows Figure 1 As shown, under visible light, the organic acid / Rose Bengal combination inactivated 99.9999% of E. coli within 8-15 min.

[0045] Embodiment 2:

[0046] Bengal rose red and citric acid were mixed in a mass ratio of 1:106, mechanically shaken to prepare a disinfectant powder, and an initial concentration of 10 6 cfu / mL of antibiotic-resistant Escherichia coli, then add 0.32g of disinfectant powder into 1.2L of water sample, shake well and expose to sunlight immediately, take samples at different time points and analyze the bacterial inactivation.

[0047] The experimental results are as follows Figure 2 As shown, under sunlight, the disinfectant powder inactivated 99.9999% of E. coli within 30 minutes.

[0048] Embodiment 3:

[0049] The conditions were the same as in Example 1, except that the concentration of the photosensitizer (Rose Bengal) was 1 mg / L, and the organic acid was citric acid.

[0050] The experimental results are as follows Figure 3 As shown, under visible light, the citric acid / Rose Bengal combination inactivated 99.9999% of E. coli within 35 min.

[0051] Embodiment 4:

[0052] The conditions were the same as those in Example 1, but without the 15 min dark treatment, and the organic acid was citric acid.

[0053] The experimental results are as follows Figure 4As shown, under visible light, the citric acid / Rose Bengal combination inactivated 99.9999% of E. coli within 25 min.

[0054] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A water disinfection powder containing Bengal rose red, characterized in that: According to the mass ratio, 1:106-265 of Rose Bengal and organic acids.

2. A water disinfection powder containing Bengal Rose Red according to claim 1, characterized in that: The photosensitizer is Bengal rose red.

3. A water disinfection powder containing Bengal Rose Red according to claim 2, characterized in that: The organic acid includes one or more of citric acid, tartaric acid and malic acid.

4. A water disinfection powder containing Bengal Rose Red according to claim 3, characterized in that: The mass ratio of the Bengal rose red to the citric acid is 1:

106.

5. A method for preparing a water disinfection powder containing Bengal Rose Red according to any one of claims 1 to 4, characterized in that: The following steps are involved: S11: Mix Rose Bengal and organic acid in a certain proportion, and shake them mechanically to obtain a disinfectant powder containing Rose Bengal.

6. A disinfectant powder containing Bengal rose obtained by the preparation method according to claim 5 for use in water disinfection, characterized in that: The following steps are involved: S21: adding disinfectant powder into the water sample in a certain proportion; S22: The water sample is then exposed to sunlight for disinfection.

7. The use of the water disinfection powder containing Bengal Rose Red according to claim 6 in water disinfection, characterized in that: The water sample is a natural water sample.

8. The use of the water disinfection powder containing Bengal Rose Red according to claim 6 in water disinfection, characterized in that: The disinfection time is 20-30 minutes.

9. The use of the water disinfection powder containing Bengal Rose Red according to claim 6 in water disinfection, characterized in that: The mass volume ratio of the disinfectant powder to the water sample is 0.30-0.34 g:1.2 L.

10. Use of the water disinfection powder containing Bengal Rose Red according to claim 9 in water disinfection, characterized in that: The mass volume ratio of the disinfectant powder to the water sample is 0.32g:1.2L.

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