Fixing device for synchronous operation of perfusion electroencephalogram of mouse

Abstract

The invention provides a fixing device for mouse electroencephalogram perfusion synchronous operation, which comprises a limiting bracket and a limiting chamber, and is characterized in that the limiting bracket is used for limiting the tail part of a mouse; a through hole is formed in the side wall of the limiting chamber in a penetrating mode, the limiting chamber is used for containing a mouse, and the tail of the mouse penetrates through the through hole to be placed on the limiting support. The tail of the mouse is fixed on the limiting bracket, so that the instability of the tail is avoided, the risk of equipment entanglement is reduced, and the duration time and the success rate of an experiment are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of fixing devices, and in particular relates to a fixing device for synchronous operation of mouse perfusion electroencephalogram. Background Art

[0002] Sleep disorders are a general term for all abnormal sleep functions, among which the relative or absolute reduction in total sleep duration is the most common and important, which is called insomnia or sleep deprivation. The reduction in sleep duration can lead to many problems such as metabolism, immunity, and emotions. In recent years, some studies have shown that restoring normal sleep after sleep deprivation cannot completely improve the damage caused by sleep deprivation, but the mechanism is unclear. In order to study sleep disorders, physiological electricity represented by EEG and EMG is crucial for recording sleep status, so in vivo physiological electricity research based on awake animals is one of the key technologies.

[0003] On the other hand, intravenous injection is a common medical intervention method, which is to pump or inject liquid substances such as blood, medicine or nutrient solution directly into the vein. Intravenous injection can be divided into transient and continuous. Transient intravenous injection is mostly injected directly into the vein with a syringe, which is a common injection method; continuous intravenous injection is implemented by intravenous drip, commonly known as "drip". In small animal experiments, continuous intravenous perfusion or tail vein administration is one of the commonly used techniques for intervening animal disease models, especially in animal experiments using propofol anesthesia.

[0004] Continuous physiological electrical recording and continuous intravenous perfusion are two common technologies in the field of life sciences, especially in the field of neuroscience. They can not only effectively observe the sleep or consciousness state of animals, but also understand the immediate effects of drugs on small animals. It has important guiding significance for the clinical mechanism of action of drugs and their later clinical transformation. However, it will be difficult to implement these two technologies on awake and freely moving animals. In particular, physiological electrical recording often takes at least several hours, or even a day and night. Due to the long recording time, the EEG recording line and the tail vein perfusion tube are easily tangled, resulting in the tail vein needle being removed or the EEG recording electrode falling off, resulting in a reduced success rate of the experiment. Summary of the invention

[0005] In view of this, the present invention provides a fixation device for synchronous operation of mouse perfusion EEG, which separates and limits the tail and body of the mouse, avoids the problem of tail vein needle dislodging or EEG recording electrode falling off, and improves the success rate of the experiment.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: The present invention provides a fixing device for synchronous operation of mouse perfusion electroencephalogram (EEG) comprising a limiting bracket and a limiting chamber, wherein the limiting bracket is used for limiting the tail of the mouse; a side wall of the limiting chamber is penetrated with a perforation, the limiting chamber is used for placing the mouse, and the tail of the mouse passes through the perforation and is placed on the limiting bracket.

[0007] Preferably, the limiting bracket includes three support rods, one end of each support rod is connected to the ends of the other two support rods to form a "Y"-shaped limiting bracket, and the tail of the mouse is placed between any two adjacent support rods.

[0008] Preferably, the three support rods are respectively a first support rod, a second support rod and a third support rod, and the distance between the first support rod and the second support rod is adapted to the tail of the mouse so that the tail of the mouse can pass through the space between the first support rod and the second support rod.

[0009] Preferably, the limiting bracket further comprises a fixing rod, one end of which is connected to the intersection of the three supporting rods, and the fixing rod is used to bind the tail of the mouse.

[0010] Preferably, the perforation is a vertical hole that penetrates the top and bottom of the side wall of the limiting chamber. Strip holes.

[0011] Preferably, the limiting chamber comprises a cylinder with open top and bottom and a bottom cover. The bottom cover and the cylinder are connected via a connecting piece, and the through hole is arranged along the height direction of the cylinder.

[0012] Preferably, the gap between the bottom cover and the cylinder is slightly larger than the tail of the mouse. The diameter at one end near the body.

[0013] Preferably, the connecting member comprises a plurality of connecting rods, and the plurality of connecting rods are arranged at intervals. Used to connect the bottom cover and the cylinder.

[0014] Preferably, the bottom cover is provided with a plurality of bases at intervals.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The invention avoids the instability of the tail by fixing the mouse tail on the limiting bracket, thereby reducing the risk of equipment entanglement and improving the duration and success rate of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the structure of a limiting bracket provided in one embodiment of the present invention; Figure 2An assembly diagram of a position limiting bracket provided in one embodiment of the present invention; Figure 3 A side view of a limiting chamber provided in one embodiment of the present invention; Figure 4 This is an assembly diagram of a fixation device for synchronous operation of mouse perfusion EEG provided by one embodiment of the present invention.

[0017] Figure numerals: a fixing device 100 for synchronous operation of EEG perfusion in mice, a limiting bracket 1, a support rod 11, a fixing rod 12, a limiting chamber 2, a cylinder 21, a through hole 211, a bottom cover 22, a base 221, and a connecting piece 23. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0019] Sleep disorders are a general term for all abnormal sleep functions, among which the relative or absolute reduction in total sleep duration is the most common and important, which is called insomnia or sleep deprivation. The reduction in sleep duration can lead to many problems such as metabolism, immunity, and emotions. In recent years, some studies have shown that restoring normal sleep after sleep deprivation cannot completely improve the damage caused by sleep deprivation, but the mechanism is unclear. In order to study sleep disorders, physiological electricity represented by EEG and EMG is crucial for recording sleep status, so in vivo physiological electricity research based on awake animals is one of the key technologies.

[0020] On the other hand, intravenous injection is a common medical intervention method, which is to pump or inject liquid substances such as blood, medicine or nutrient solution directly into the vein. Intravenous injection can be divided into transient and continuous. Transient intravenous injection is mostly injected directly into the vein with a syringe, which is a common injection method; continuous intravenous injection is implemented by intravenous drip, commonly known as "drip". In small animal experiments, continuous intravenous perfusion or tail vein administration is one of the commonly used techniques for intervening animal disease models, especially in animal experiments using propofol anesthesia.

[0021] Continuous physiological electrical recording and continuous intravenous perfusion are two common technologies in the field of life sciences, especially in the field of neuroscience. They can not only effectively observe the sleep or consciousness state of animals, but also understand the immediate effects of drugs on small animals. It has important guiding significance for the clinical mechanism of action of drugs and their later clinical transformation. However, it will be difficult to implement these two technologies on awake and freely moving animals. In particular, physiological electrical recording often takes at least several hours, or even a day and night. Due to the long recording time, the EEG recording line and the tail vein perfusion tube are easily tangled, resulting in the tail vein needle being removed or the EEG recording electrode falling off, resulting in a reduced success rate of the experiment.

[0022] In order to solve the above technical problems, Figure 1-4 The present invention provides a fixing device 100 for synchronous operation of mouse perfusion electroencephalogram (EEG) including a limiting bracket 1 and a limiting chamber 2. The limiting bracket 1 is used to limit the tail of the mouse. A through-hole 211 is provided through the side wall of the limiting chamber 2. The limiting chamber 2 is used to place the mouse. The tail of the mouse passes through the through-hole 211 and is placed on the limiting bracket 1.

[0023] In the above technical solution, the main function of the limiting bracket 1 is to limit the tail of the mouse and prevent the tail from accidentally moving during the experiment. The limiting bracket 1 ensures that the mouse's tail is fixed and reduces the risk of the tail vein needle being removed. The limiting chamber 2 is a container for placing mice. The side wall of the container is designed with a perforation 211, allowing the mouse's tail to pass through the perforation 211 and be placed on the limiting bracket 1. This design can effectively fix the mouse's tail and ensure that the tail vein perfusion tube can work stably.

[0024] The present invention fixes the mouse tail on the limiting bracket 1, thereby avoiding the instability of the tail, reducing the risk of equipment entanglement and improving the duration and success rate of the experiment.

[0025] Furthermore, the position limiting bracket 1 includes three support rods 11, one of each support rod 11 The end is connected to the ends of the other two support rods 11 to form a "Y"-shaped limiting bracket 1, and the mouse's tail is placed between any two adjacent support rods 11. The purpose of this design is to provide stable support through the "Y"-shaped structure of the bracket, so that the mouse's tail is fixed in a relatively stable position to avoid unnecessary movement or swinging of the tail during the experiment. The common problem of equipment falling off or failure caused by tail swinging in experiments is avoided. This design enhances the stability and operability of the experiment and improves the success rate of the experiment. In some embodiments, the three support rods 11 are respectively the first support rod 11, the second support rod 11, and the third support rod 11, and the distance between the first support rod 11 and the second support rod 11 is adapted to the mouse's tail so that the mouse's tail passes through the space between the first support rod 11 and the second support rod 11.

[0026] Furthermore, the position limiting bracket 1 further comprises a fixing rod 12, one side of the fixing rod 12 The end is connected to the intersection of the three support rods 11, and the fixing rod 12 is used to bind the tail of the mouse. By adding the fixing rod 12, the stable fixation of the mouse tail is further strengthened, the instability of the tail during the experiment is effectively avoided, and the risk of tail vein needle removal and EEG electrode falling off is reduced. This improvement improves the continuity, accuracy and reliability of the experiment, and greatly improves the success rate and data quality of physiological electrical recording and intravenous perfusion experiments.

[0027] Furthermore, the through hole 211 is a hole that vertically penetrates the top of the side wall of the limiting chamber 2. The strip-shaped perforations 211 vertically running through the side wall of the limiting chamber 2 can effectively guide and fix the mouse's tail to prevent the tail from excessive movement or falling off. This design enables the tail to be stably placed on the limiting bracket 1, enhances the stability of the equipment during the experiment, and improves the success rate and sustainability of the experiment. In addition, the simple design improves manufacturing efficiency and reduces costs, while also helping to reduce stress on mice and ensure the reliability and accuracy of the experiment.

[0028] Furthermore, the limiting chamber 2 includes a cylinder 21 with open top and bottom and a bottom cover. 22, the bottom cover 22 and the barrel 21 are connected by a connector 23, and the perforation 211 is arranged along the height direction of the barrel 21. The limit chamber 2 of this structural design is provided with an efficient and stable space to fix the mouse tail by the combination of the barrel 21 and the bottom cover 22, the open design, and the perforation 211 in the vertical direction, ensuring the stability of the tail in the experiment. The use of the connector 23 ensures the firmness and disassembly of the assembly, and is convenient for operation and cleaning. This design not only improves the stability of the experimental equipment and the accuracy of the experiment, but also enhances the flexibility of the experiment and the operability of long-term use.

[0029] Furthermore, the gap between the bottom cover 22 and the cylinder 21 is slightly larger than the small The diameter of the mouse's tail near one end of the body. The slightly larger than mentioned in the present invention means that the gap is slightly larger than the thickest part of the mouse's tail, 0.2-0.5cm. By designing a gap slightly larger than the diameter of the mouse's tail near one end of the body, the space between the bottom cover 22 and the barrel 21 ensures that the tail can pass smoothly and be stably fixed. At the same time, when the mouse is active, the gap between the bottom cover 22 and the barrel 21 facilitates the movement of the tail. This design can effectively avoid oppression or discomfort of the tail, and also prevent the tail from being overactive, thereby ensuring the stability of the equipment and the accuracy of the data during the experiment. In addition, the design adapts to the tail sizes of different mice, improving the flexibility and operating efficiency of the experiment.

[0030] Furthermore, the connecting member 23 includes a plurality of connecting rods, and the plurality of connecting rods are spaced The bottom cover 22 and the barrel 21 are connected by a plurality of connecting rods arranged at intervals to connect the bottom cover 22 and the barrel 21, and the design provides a more stable structure, which enhances the reliability and durability of the connection. At the same time, the connecting rod makes the device easier to disassemble and assemble, reduces manufacturing costs, and provides good flexibility. This design not only ensures the stable fixation of the mouse tail, but also optimizes the cleaning and ventilation of the equipment, which helps to improve the efficiency during the experiment and the long-term stability of the equipment.

[0031] Furthermore, the bottom cover 22 is provided with a plurality of bases 221 at intervals. By providing a plurality of bases 221 at intervals on the bottom cover 22, not only the stability and durability of the limit chamber 2 structure can be improved, but also the bottom cover 22 can be prevented from being damaged due to friction or excessive pressure. The base 221 design improves cleaning efficiency, enhances ventilation, and reduces interference caused by vibration or uneven surfaces. Overall, this design ensures experimental accuracy while also improving the service life and ease of operation of the equipment.

[0032] Exemplarily, the present invention provides a method for fixing a mouse on the limiting bracket 1, The steps are as follows: anesthetize the experimental mouse, pass the mouse tail through between the first support rod 11 and the second support rod 11, and use medical tape to wrap the mouse tail and the fixing rod 12 together and fix them.

[0033] Exemplarily, the present invention provides a method for using a fixation device 100 for synchronous operation of perfusion EEG of a mouse, comprising the following steps: Step 1, anesthetize the mouse; Step 2, connecting the mouse to a physiological electrical collection device; Step 3, place the mouse except the fixed limiting bracket 1 into the cylinder 21 from the top of the cylinder 21, and at the same time, the tail of the mouse also moves downward from the upper part of the through hole 211 to the lower part. When the mouse is located in the limiting chamber 2, the tail of the mouse is located in the gap between the cylinder 21 and the bottom cover 22.

[0034] Step 4, connect the tail vein continuous infusion device to the mouse; Step 5: When the small animal wakes up, the tail of the small animal can move in the gap between the cylinder 21 and the bottom cover 22, and drug pumping and signal collection are performed at the same time.

[0035] The specific raw materials not described in the present invention are all existing materials and can be directly purchased from the market.

[0036] The above are only preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fixation device for synchronous operation of mouse perfusion EEG, characterized in that: include: A limiting bracket, used to limit the tail of the mouse; The limiting chamber has a side wall with a perforation, and the limiting chamber is used to place mice. The tail of the mouse is passed through the perforation and placed on the limiting bracket.

2. The fixation device for synchronous operation of mouse perfusion EEG according to claim 1, characterized in that The feature is that the limiting bracket includes three support rods, one end of each support rod is connected to the ends of the other two support rods to form a "Y"-shaped limiting bracket, and the tail of the mouse is placed between any two adjacent support rods.

3. The fixation device for synchronous operation of mouse perfusion EEG according to claim 2, characterized in that The invention is characterized in that the three support rods are respectively a first support rod, a second support rod and a third support rod, and the distance between the first support rod and the second support rod is adapted to the tail of a mouse so that the tail of the mouse can pass through the space between the first support rod and the second support rod.

4. The fixation device for synchronous operation of mouse perfusion EEG according to claim 2, wherein The characteristic is that the limiting bracket also includes a fixing rod, one end of which is connected to the intersection of the three support rods, and the fixing rod is used to bind the tail of the mouse.

5. The fixation device for synchronous operation of mouse perfusion EEG according to claim 1, wherein The characteristic lies in that the through hole is a strip-shaped hole vertically penetrating the top and bottom of the side wall of the limiting chamber.

6. The fixation device for synchronous operation of mouse perfusion EEG according to claim 5, wherein The characteristic is that the limiting chamber comprises a cylinder with open top and bottom and a bottom cover, the bottom cover and the cylinder are connected by a connecting piece, and the through hole is arranged along the height direction of the cylinder.

7. The fixation device for synchronous operation of mouse perfusion EEG according to claim 6, wherein The invention is characterized in that the gap between the bottom cover and the cylinder is slightly larger than the diameter of the mouse's tail near one end of the body.

8. The fixation device for synchronous operation of mouse perfusion EEG according to claim 6, wherein The characteristic is that the connecting member comprises a plurality of connecting rods, and the plurality of connecting rods are arranged at intervals to connect the bottom cover and the cylinder.

9. The fixation device for synchronous operation of mouse perfusion EEG according to claim 5, wherein The feature is that the bottom cover is provided with a plurality of bases at intervals.

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