A medicine-food formula for preventing stroke

Abstract

The invention discloses a medicine-food formula for preventing stroke in the field of medicine-food formula, and the medicine-food formula includes: 5-60 grams of Astragalus or extract; 5-60 grams of Radix Pseudostellariae or extract; 5-60 grams of Poria or extract; 5-60 grams of White Peony Root or extract; 5-60 grams of Radix Rehmanniae or extract; 5-60 grams of Polygonatum or extract; 5-60 grams of Rhizoma Anemarrhenae or extract; 5-60 grams of Atractylodes or extract; 5-60 grams of Licorice or extract; 5-60 grams of Achyranthes Bidentatae or extract; 5-60 grams of Imperatae cylindricae or extract; 5-60 grams of Morus alba or extract; 5-60 grams of Semen Cassiae or extract. The optimized preparation method of the invention fully extracts the effective ingredients of medicinal materials, improves the absorption and utilization rate of human body, makes each component act synergistically, prevents stroke in multiple ways, and provides safe and effective prevention for high-risk groups.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine-food homologous formula, and specifically refers to a medicine-food homologous formula for preventing stroke. Background Art

[0002] As people pay more and more attention to health, medicine and food products are widely welcomed because of their health care and certain conditioning effects. However, some existing medicine and food formulas have problems such as low extraction rate of effective ingredients, poor preparation stability, and poor taste during the preparation process, which affect their promotion and application. The present invention aims to provide a new preparation method to overcome these shortcomings and better play the advantages of medicine and food formulas. Summary of the invention

[0003] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a medicine and food formula for preventing stroke.

[0004] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The present invention proposes a medicine-food formula for preventing stroke, and the medicine-food formula comprises:

[0005] Astragalus or extract 5-60g;

[0006] 5-60g of Pseudostellaria heterophylla or extract;

[0007] Poria cocos or extract 5-60g;

[0008] 5-60g white peony root or extract;

[0009] 5-60g of raw earth or extract;

[0010] 5-60g of Polygonatum odoratum or its extract;

[0011] 5-60g of bamboo shavings or extract;

[0012] 5-60 g of Atractylodes macrocephala or extract;

[0013] Licorice root or extract 5-60 g;

[0014] Achyranthes bidentata or extract 5-60 g;

[0015] Imperata root or extract 5-60 g;

[0016] 5-60 g of mulberry branches or extract;

[0017] 5-60 grams of Cassia seed or extract.

[0018] Preferably, the medicine-food formula comprises:

[0019] Astragalus or extract 15-40 grams;

[0020] 15-40g of Pseudostellaria heterophylla or extract;

[0021] Poria cocos or extract 15-40 grams;

[0022] White peony root or extract 15-40g;

[0023] 15-40g of raw earth or extract;

[0024] 15-40 grams of Polygonatum odoratum or its extract;

[0025] 15-40g bamboo shavings or extract;

[0026] Atractylodes macrocephala or extract 15-40 g;

[0027] Licorice root or extract 15-40 g;

[0028] Achyranthes bidentata or extract 15-40 g;

[0029] Imperata root or extract 15-40 g;

[0030] 15-40 g of mulberry branches or extract;

[0031] 15-40 grams of Cassia seed or extract.

[0032] As further preferred, the medicine-food homologous formula comprises:

[0033] Astragalus or extract 20-30 grams;

[0034] 20-30g of Pseudostellaria heterophylla or extract;

[0035] 20-30 g of Poria cocos or extract;

[0036] 20-30g white peony root or extract;

[0037] 20-30g of raw earth or extract;

[0038] 20-30 grams of Polygonatum odoratum or its extract;

[0039] 20-30g bamboo shavings or extract;

[0040] 20-30 g of Atractylodes macrocephala or extract;

[0041] Licorice root or extract 20-30 grams;

[0042] 20-30g of Achyranthes bidentata or extract;

[0043] Imperata root or extract 20-30 g;

[0044] 20-30 g of mulberry branches or extract;

[0045] 20-30 grams of Cassia seed or extract.

[0046] In the present invention, the preparation method of the medicine-food formula comprises the following steps:

[0047] Step 1: Preprocessing:

[0048] Remove impurities from the above medicinal materials, wash them and dry them;

[0049] Cut Astragalus, Radix Pseudostellariae, Poria, White Peony Root, Radix Rehmanniae, Polygonatum, Rhizoma Anemarrhenae, Atractylodes, Licorice, Achyranthes, Imperata Root, Morus Alpiniae, and Semen Cassiae into thin slices or small sections respectively and set aside;

[0050] Extraction of active ingredients:

[0051] Take the above-pretreated medicinal materials, add purified water according to the solid-liquid ratio, and soak for 1-2 hours; put the soaked medicinal materials and water in a multifunctional extraction tank, heat to boiling and then maintain a slightly boiling state for extraction for 1-2 hours, filter, and collect the extract; add purified water to the filter residue according to the solid-liquid ratio, repeat the extraction once, and combine the two extracts;

[0052] Step 2: Concentration and drying:

[0053] The combined extracts are concentrated under reduced pressure to a clear paste having a relative density of 1.10-1.20 (60° C.); the clear paste is spray-dried to obtain a dry powder;

[0054] Step 3: Granulation and molding:

[0055] Adding a proper amount of auxiliary materials to the dry powder, the auxiliary materials may be one or more of dextrin, sucrose, and mannitol; adding a proper amount of 80%-90% ethanol as a wetting agent to make a soft material, granulating it through a swing granulator, and passing it through a 10-20 mesh sieve to obtain wet granules;

[0056] Step 4: Drying and packaging of whole grains:

[0057] The wet granules are placed in a hot air circulation oven; the dried granules are sieved through a 10-20 mesh screen to remove fine powder and agglomerated particles;

[0058] The whole granules are packaged according to the specifications to obtain the finished product.

[0059] Preferably, the material-liquid ratio after pretreatment in step 1 is 1:8-1:12 (g / mL).

[0060] Preferably, the solid-to-liquid ratio of the filter residue in step one is 1:6-1:10 (g / mL).

[0061] Preferably, the drying conditions in step 2 are: inlet air temperature 160-180°C, outlet air temperature 80-100°C.

[0062] Wherein, in step three, the ratio of auxiliary materials to drug powder is 1:1-1:3.

[0063] In addition, the drying condition in step 4 is: drying at 50-70° C. until the moisture content is less than 6%.

[0064] The beneficial effects achieved by the present invention are as follows:

[0065] The present invention adopts a variety of drugs in combination, wherein:

[0066] Astragalus and Pseudostellaria enhance the body's resistance and immunity, regulate the circulation of Qi and blood, maintain blood vessel elasticity, and reduce the risk of disease; Poria and Atractylodes strengthen the spleen and eliminate dampness, eliminate the source of phlegm, and prevent phlegm and dampness from blocking the meridians.

[0067] White peony root nourishes liver blood, raw rehmannia root clears heat and cools blood, improves blood rheology, polygonatum odoratum nourishes yin and moistens the lungs, protects the vascular endothelium, and bamboo shavings clear heat and resolves phlegm, which together reduce blood viscosity and inhibit the formation of atherosclerosis.

[0068] Licorice harmonizes all the medicines, enhances the body's resistance, and reduces damage to blood vessels caused by inflammation; Achyranthes bidentata removes blood stasis and dredges the meridians, guides blood downward, nourishes the liver and kidneys, and reduces the pressure of qi and blood rushing upward; Imperata cylindrica cools blood and stops bleeding, clears away heat and promotes diuresis, stabilizes blood pressure, and prevents hemorrhagic stroke; Morus alba dredges meridians and promotes the flow of water and vapor, avoiding qi and blood stasis and endogenous phlegm and dampness; Semen Cassiae calms the liver and suppresses yang, preventing stroke caused by internal liver wind.

[0069] The present invention improves the extraction rate of effective ingredients of each edible and medicinal material by optimizing extraction process parameters, such as material-liquid ratio, extraction time and extraction times, and ensures the efficacy of the product. Spray drying and suitable granulation processes are adopted, and the obtained granules have good fluidity, solubility and stability, and are convenient for storage and administration. The selection and proportion of auxiliary materials are optimized, the taste of the product is improved, and the compliance of patients is improved.

[0070] The optimized preparation method can fully extract the effective ingredients of medicinal materials, improve the absorption and utilization rate of the human body, make the various ingredients work synergistically, prevent stroke in multiple ways, and provide safe and effective prevention methods for high-risk groups, with broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0071] Figure 1 Neurological deficit score of a food-drug formula for stroke prevention;

[0072] Figure 2 A graph showing the results of measuring the cerebral infarction volume of a medicine-food formula for preventing stroke;

[0073] Figure 3 A graph showing the results of blood lipid level testing for a food-medicine formula for preventing stroke.

[0074] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0075] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0076] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0077] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0078] Example 1

[0079] Weigh 25 grams of Astragalus, 25 grams of Pseudostellaria, 25 grams of Poria, 25 grams of White Peony, 25 grams of Rehmannia, 25 grams of Polygonatum, 25 grams of Rhizoma Anemarrhenae, 25 grams of Atractylodes, 25 grams of Licorice, 25 grams of Achyranthes, 25 grams of Imperata Rhizoma, and 25 grams of Morus, wash them respectively, dry them, and cut them into thin slices or small pieces.

[0080] After mixing the above medicinal materials, add purified water at a solid-liquid ratio of 1:10 (g / mL) and soak for 1.5 hours.

[0081] Transfer to a multifunctional extraction tank, heat to boiling and maintain slight boiling for 1.5 hours, filter and collect the extract; add purified water to the residue at a solid-liquid ratio of 1:8 (g / mL), repeat the extraction once, and combine the two extracts.

[0082] Weigh 25 grams of Cassia Seed Extract for later use.

[0083] The combined extracts were concentrated under reduced pressure to a clear paste with a relative density of 1.15 (60°C), and then spray-dried with an inlet air temperature of 170°C and an outlet air temperature of 90°C to obtain a dry powder.

[0084] Dextrin and sucrose (1:1) were added to the dry powder, the ratio of excipients to drug powder was 1:2, and an appropriate amount of 85% ethanol was added to make a soft material, which was granulated by a swing granulator and passed through a 15-mesh sieve to obtain wet granules.

[0085] The wet granules were dried in a hot air circulation oven at 60° C. to a moisture content of 5%, and then sieved through a 15-mesh sieve to size the granules. The granules were packaged in a bag of 10 g to obtain finished granules.

[0086] Example 2

[0087] Weigh 60 grams of Astragalus, 60 grams of Pseudostellaria, 60 grams of Poria, 60 grams of White Peony, 60 grams of Rehmannia, 60 grams of Polygonatum, 60 grams of Rhizoma Anemarrhenae, 60 grams of Atractylodes, 60 grams of Licorice, 60 grams of Achyranthes, 60 grams of Imperata Rhizoma, and 60 grams of Morus, wash them respectively, dry them, and cut them into thin slices or small pieces.

[0088] After mixing the above medicinal materials, add purified water at a solid-liquid ratio of 1:10 (g / mL) and soak for 1.5 hours.

[0089] Transfer to a multifunctional extraction tank, heat to boiling and maintain slight boiling for 1.5 hours, filter and collect the extract; add purified water to the residue at a solid-liquid ratio of 1:8 (g / mL), repeat the extraction once, and combine the two extracts.

[0090] Weigh 25 grams of Cassia Seed Extract for later use.

[0091] The combined extracts were concentrated under reduced pressure to a clear paste with a relative density of 1.15 (60°C), and then spray-dried with an inlet air temperature of 170°C and an outlet air temperature of 90°C to obtain a dry powder.

[0092] Dextrin and sucrose (1:1) were added to the dry powder, the ratio of excipients to drug powder was 1:2, and an appropriate amount of 85% ethanol was added to make a soft material, which was granulated by a swing granulator and passed through a 15-mesh sieve to obtain wet granules.

[0093] The wet granules were dried in a hot air circulation oven at 60° C. to a moisture content of 5%, and then sieved through a 15-mesh sieve to size the granules. The granules were packaged in a bag of 10 g to obtain finished granules.

[0094] Example 3

[0095] Weigh 5 grams of Astragalus, 5 grams of Pseudostellaria, 5 grams of Poria, 5 grams of White Peony Root, 5 grams of Rehmannia, 5 grams of Polygonatum, 5 grams of Rhizoma Anemarrhenae, 5 grams of Atractylodes, 5 grams of Licorice, 5 grams of Achyranthes, 5 grams of Imperata Rhizoma, and 5 grams of Morus, wash them separately, dry them, and cut them into thin slices or small pieces.

[0096] After mixing the above medicinal materials, add purified water at a solid-liquid ratio of 1:10 (g / mL) and soak for 1.5 hours.

[0097] Transfer to a multifunctional extraction tank, heat to boiling and maintain slight boiling for 1.5 hours, filter and collect the extract; add purified water to the residue at a solid-liquid ratio of 1:8 (g / mL), repeat the extraction once, and combine the two extracts.

[0098] Weigh 25 grams of Cassia Seed Extract for later use.

[0099] The combined extracts were concentrated under reduced pressure to a clear paste with a relative density of 1.15 (60°C), and then spray-dried with an inlet air temperature of 170°C and an outlet air temperature of 90°C to obtain a dry powder.

[0100] Dextrin and sucrose (1:1) were added to the dry powder, the ratio of excipients to drug powder was 1:2, and an appropriate amount of 85% ethanol was added to make a soft material, which was granulated by a swing granulator and passed through a 15-mesh sieve to obtain wet granules.

[0101] The wet granules were dried in a hot air circulation oven at 60° C. to a moisture content of 5%, and then sieved through a 15-mesh sieve to size the granules. The granules were packaged in a bag of 10 g to obtain finished granules.

[0102] Experimental example

[0103] Effect of a medicine-food formula for preventing stroke

[0104] 1. Experimental animals and groups

[0105] Twenty clean-grade male SD rats weighing 250-300 g were selected and randomly divided into a normal control group, a model control group, a positive control group (aspirin) and an experimental group (administered the medicine-food homologous formulation of the present invention), with 5 rats in each group.

[0106] 2. Modeling method

[0107] The middle cerebral artery occlusion (MCAO) model was prepared by suture method to simulate stroke. Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (350 mg / kg), fixed in supine position, and a midline incision was made in the neck to separate the right common carotid artery (CCA), external carotid artery (ECA) and internal carotid artery (ICA). The proximal ends of CCA and ECA were ligated, a small incision was made at the distal end of CCA, and the pre-prepared nylon suture was inserted into ICA through CCA to the beginning of the middle cerebral artery to block blood flow, and the skin was sutured to create a focal cerebral ischemia model.

[0108] 3. Method of administration

[0109] The normal control group and the model control group were given an equal volume of physiological saline by gavage, the positive control group was given aspirin by gavage, and the experimental group was given the medicine and food homologous formulation of the present invention by gavage, once a day, for 10 consecutive weeks.

[0110] Test parameters:

[0111] (I) Neurological deficit score

[0112] 24 hours after modeling and every week after drug administration, the neurological function of rats was evaluated using the Longa scoring method:

[0113] 0 points: no neurological deficit symptoms;

[0114] 1 point: unable to fully extend the contralateral forepaw;

[0115] 2 points: turning to the opposite side;

[0116] 3 points: falling to the opposite side;

[0117] 4 points: Unable to walk spontaneously and loss of consciousness.

[0118] (II) Determination of cerebral infarction volume

[0119] After the administration, the rats were killed, the brains were removed and made into continuous brain slices, and the cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Image analysis software was used to calculate the percentage of cerebral infarction volume to the total brain volume.

[0120] (III) Blood lipid level detection

[0121] Abdominal aorta blood was also collected, serum was separated, and the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were detected using an automatic biochemical analyzer.

[0122] Results analysis, such as Figure 1 As shown, compared with the model control group, the experimental group had significant improvements in neurological deficit score, cerebral infarction volume and blood lipid levels, indicating that the medicine and food formula preparation of the present invention has a certain effect in preventing stroke, and in some aspects is similar to the effect of the positive control group (aspirin), but the specific mechanism needs further in-depth study.

[0123] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0124] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A medicine-food formula for preventing stroke, characterized in that: The medicine-food homologous formula comprises: Astragalus or extract 5-60g; 5-60g of Pseudostellaria heterophylla or extract; Poria cocos or extract 5-60g; 5-60g white peony root or extract; 5-60g of raw earth or extract; 5-60g of Polygonatum odoratum or its extract; 5-60g of bamboo shavings or extract; 5-60 g of Atractylodes macrocephala or extract; Licorice root or extract 5-60 g; Achyranthes bidentata or extract 5-60 g; Imperata root or extract 5-60 g; 5-60 g of mulberry branches or extract; 5-60 grams of Cassia seed or extract.

2. A medicine-food formula for preventing stroke according to claim 1, characterized in that: The medicine-food homologous formula comprises: Astragalus or extract 15-40 grams; 15-40g of Pseudostellaria heterophylla or extract; Poria cocos or extract 15-40 grams; White peony root or extract 15-40g; 15-40g of raw earth or extract; 15-40 grams of Polygonatum odoratum or its extract; 15-40g bamboo shavings or extract; Atractylodes macrocephala or extract 15-40 g; Licorice root or extract 15-40 g; Achyranthes bidentata or extract 15-40 g; Imperata root or extract 15-40 g; 15-40 g of mulberry branches or extract; 15-40 grams of Cassia seed or extract.

3. A medicine-food formula for preventing stroke according to claim 2, characterized in that: The medicine-food homologous formula comprises: Astragalus or extract 20-30 grams; 20-30g Pseudostellariae Radix or extract; 20-30 g of Poria cocos or extract; 20-30g white peony root or extract; 20-30g of raw earth or extract; 20-30 grams of Polygonatum odoratum or its extract; 20-30g bamboo shavings or extract; 20-30 g of Atractylodes macrocephala or extract; Licorice root or extract 20-30 g; 20-30g of Achyranthes bidentata or extract; Imperata root or extract 20-30 g; 20-30 g of mulberry branches or extract; 20-30 grams of Cassia seed or extract.

4. A medicine-food formula for preventing stroke according to claim 3, characterized in that: The preparation method of the medicine-food homologous formula comprises the following steps: Step 1: Preprocessing: Remove impurities from the above medicinal materials, wash them and dry them; Cut Astragalus, Radix Pseudostellariae, Poria, White Peony Root, Radix Rehmanniae, Polygonatum, Rhizoma Anemarrhenae, Atractylodes, Licorice, Achyranthes, Imperata Root, Morus Alpiniae, and Semen Cassiae into thin slices or small sections respectively and set aside; Extraction of active ingredients: Take the above-pretreated medicinal materials, add purified water according to the solid-liquid ratio, and soak for 1-2 hours; put the soaked medicinal materials and water in a multifunctional extraction tank, heat to boiling and then maintain a slightly boiling state for extraction for 1-2 hours, filter, and collect the extract; add purified water to the filter residue according to the solid-liquid ratio, repeat the extraction once, and combine the two extracts; Step 2: Concentration and drying: The combined extracts are concentrated under reduced pressure to a clear paste having a relative density of 1.10-1.20 (60° C.); the clear paste is spray-dried to obtain a dry powder; Step 3: Granulation and molding: Adding a proper amount of auxiliary materials to the dry powder, the auxiliary materials may be one or more of dextrin, sucrose, and mannitol; adding a proper amount of 80%-90% ethanol as a wetting agent to make a soft material, granulating it through a swing granulator, and passing it through a 10-20 mesh sieve to obtain wet granules; Step 4: Drying and packaging of whole grains: The wet granules are placed in a hot air circulation oven; the dried granules are sieved through a 10-20 mesh screen to remove fine powder and agglomerated particles; The whole granules are packaged according to the specifications to obtain the finished product.

5. The food-drug formula for preventing stroke according to claim 4, characterized in that: The material-liquid ratio after pretreatment in step 1 is 1:8-1:12 (g / mL).

6. The food-drug formula for preventing stroke according to claim 5, characterized in that: The solid-liquid ratio of the step one filter residue is 1:6-1:10 (g / mL).

7. The food-drug formula for preventing stroke according to claim 6, characterized in that: The drying conditions in step 2 are: inlet air temperature 160-180°C, outlet air temperature 80-100°C.

8. The food-drug formula for preventing stroke according to claim 7, characterized in that: In step 3, the ratio of excipients to drug powder is 1:1-1:

3.

9. The food-drug formula for preventing stroke according to claim 8, characterized in that: The drying conditions in step 4 are: drying at 50-70° C. until the moisture content is less than 6%.

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