Plant underwater cutting sampling device and sampling method

Abstract

The invention relates to a plant underwater cut-off sampling device and a sampling method, and belongs to the field of plant sampling equipment.The plant underwater cut-off sampling device comprises a water storage device, the side wall of the water storage device is provided with a first inlet groove penetrating through the wall thickness of the water storage device along the height of the water storage device, and the length of the first inlet groove is consistent with the height of the water storage device; a second introduction groove penetrating through the wall thickness of the bottom wall is formed in the bottom wall of the water storage device, one end of the second introduction groove starts from the center of the bottom wall of the water storage device, the other end of the second introduction groove extends towards the first introduction groove and is communicated with the first introduction groove, and the first introduction groove is matched with the second introduction groove and used for enabling a plant sample to enter the water storage device; the sealing piece is detachably connected with the water storage device, and the sealing piece is used for blocking the first inlet groove and the second inlet groove; the fixing part is used for fixing the plant sample entering the water storage device. The underwater cutting sampling step can be directly performed under the condition of keeping the original state, so that the xylem tension of the plant sample is quickly released, other embolism errors are not introduced, and the measurement error caused by sampling is effectively reduced.

Description

Technical Field

[0001] The present application relates to the field of plant sampling equipment, and in particular to an underwater plant cutting sampling device and a sampling method. Background Art

[0002] In the xylem of plants, water is transmitted upward through the water flow tension caused by transpiration. This is a key process to maintain the normal physiological state of plants and is of great significance to plant survival, growth and development. The water flow in the xylem is in a metastable state under tension. When the tension is too large or there is other interference or damage, the water is easy to vaporize, causing embolism in the transmission pipeline, reducing the water transmission capacity of the xylem, and thus leading to decreased productivity, tissue dehydration damage and even death. Accurately assessing the degree of xylem embolism and its dynamic changes is an important basic work for plant hydraulic research, and can also provide reference for irrigation decisions, disaster prevention and mitigation, and variety selection. A large number of previous studies have confirmed that when there is tension in the water flow in the xylem, directly cutting off the plant sample in the air will introduce air and aggravate the embolism, which will cause serious errors in the subsequent determination of the degree of embolism. Therefore, when sampling the stem, it is often necessary to cut it underwater to release the tension so as to maintain the original degree of embolism.

[0003] The two most commonly used methods are as follows: (1) Place water in a shallow basin, immerse the front end of plant samples such as branches in the water, or bend them and partially immerse them in water before cutting them off. This method is only suitable for soft branches. It cannot be used when the stems of woody plants are very hard or when some of the plant stems are bent and easily damaged. It is also difficult to use this method to cut plant samples underwater for upright plants such as corn and sorghum. In addition, this method usually requires two people to work together, and the sampling efficiency is low. (2) Wrap part of the plant branches and leaves in advance to inhibit transpiration and reduce the tension of the xylem, then cut the stem directly in the air, and then cut part of the stem segment underwater for measurement. This method takes a certain amount of time to inhibit transpiration, and it is very likely that the original degree of xylem embolism has been changed. In addition, the sample is still cut in the air, which will also artificially introduce xylem embolism and cause subsequent measurement errors.

[0004] Currently, there is a lack of suitable tools and efficient sampling methods for underwater cutting of plant samples, which can collect a large number of plant samples of different plant types underwater in a short period of time. Summary of the invention

[0005] In order to collect more plant samples of different plant types underwater in a short period of time, the present application provides a plant underwater cutting sampling device and a sampling method.

[0006] In the first aspect, the present application provides a plant underwater cutting sampling device, which adopts the following technical solution:

[0007] A plant underwater cutting sampling device, comprising:

[0008] A water reservoir, wherein a first access groove penetrating through the wall thickness of the water reservoir is provided on the side wall of the water reservoir along the height thereof, the length of the first access groove being consistent with the height of the water reservoir, a second access groove penetrating through the wall thickness of the bottom wall is provided on the bottom wall of the water reservoir, one end of the second access groove starts at the center of the bottom wall of the water reservoir, and the other end extends toward the first access groove and is connected with the first access groove, the first access groove cooperates with the second access groove to allow plant samples to enter the water reservoir;

[0009] A sealing member, the sealing member is detachably connected to the water storage container and is used to seal the first inlet groove and the second inlet groove;

[0010] A fixing member is used to fix the plant sample entering the water reservoir.

[0011] Optionally, the sealing member is an elastic strip, which includes an inserted portion and an exposed portion, the inserted portion and the exposed portion are fixedly connected, the inserted portion is located in the entire first access groove and the entire second access groove, and the exposed portion is fitted with an outer wall of the water storage container on one side close to the inserted portion.

[0012] Optionally, the edges of the vessel wall on both sides of the first feed slot and the edges of the vessel wall on both sides of the second feed slot are fixedly connected with arc-shaped protrusions, and corresponding positions of the exposed portion are provided with grooves for accommodating the arc-shaped protrusions.

[0013] Optionally, the fixing member includes a sealing belt and a fixing clip, one end of the sealing belt is fixedly connected to the outer bottom wall of the water reservoir, and the other end extends to the center of the bottom wall of the water reservoir and then bends in a direction away from the bottom wall of the water reservoir; one end of the sealing member located at the center of the bottom wall of the water reservoir is bent in a direction away from the bottom wall of the water reservoir, and the fixing clip is simultaneously clamped between the bent portion of the sealing belt and the bent portion of the sealing member.

[0014] Optionally, the sealing tape and the sealing element are both made of latex or silicone.

[0015] Optionally, a handle is connected to the side wall of the water storage container, and the fixing clip is clamped on the handle.

[0016] Optionally, the water storage container is made of a transparent hard material.

[0017] On the other hand, the present application provides a method for underwater plant cutting sampling using any sampling device described in the first aspect above, using the following technical solution:

[0018] A method for underwater cutting and sampling of plants, comprising:

[0019] (1) allowing the plant sample to enter the water reservoir through the first and second entry grooves, and allowing a first distance below a target cut-off position of the plant sample to be located at the center of a bottom wall of the water reservoir;

[0020] (2) inserting a sealing member into the first access groove and the second access groove, wherein the sealing member extends to the center position of the bottom;

[0021] (3) Fixing the plant specimen by a fixing device;

[0022] (4) placing the water reservoir into the solution so that the liquid level of the solution is a second distance above the target cut-off position;

[0023] (5) cutting the plant sample at a target cutting position in the solution;

[0024] (6) placing a container in the water reservoir, placing the cut segments and part of the solution in the water reservoir in the solution through the container, and the cut segments of the stem are always in the solution.

[0025] Optionally, the first distance is 3-5 cm; the second distance is 3-7 cm.

[0026] Optionally, the edges of the vessel wall on both sides of the first feed groove and the edges of the vessel wall on both sides of the second feed groove are coated with vaseline.

[0027] In summary, this application includes the following beneficial technical effects:

[0028] By setting a water storage tank, a sealing member and a fixing member, the water storage tank is provided with a first access groove and a second access groove. When in use, the sealing member is released from the first access groove and the second access groove to allow the plant sample to enter the water storage tank, and the plant sample is fixed by the fixing member; then the sealing member is used to block the first access groove and the second access groove, and finally the water storage tank is placed in water or other solutions, so that the underwater cutting sampling step can be directly performed while maintaining the original state, so that the plant sample can quickly release the tension of the wood part, and other embolic errors will not be introduced, thereby effectively reducing the measurement error caused by sampling; the operation is convenient, the sampling time is short, and the sampling can be completed by one person; there is no need to bend the plant sample, and it is applicable to a wide range of plant types. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the three-dimensional structure of a plant underwater cutting and sampling device according to an embodiment of the present application;

[0030] Figure 2 This is a schematic diagram of the working state of a plant underwater cutting and sampling device according to an embodiment of the present application;

[0031] Figure 3It is a cross-sectional view of a sealing member in a plant underwater cutting and sampling device according to an embodiment of the present application when the sealing member is in a vertical state;

[0032] Figure 4 It is a cross-sectional view of another plant underwater cutting and sampling device in an embodiment of the present application when the sealing member is in a vertical state;

[0033] Explanation of the reference numerals: 1. water storage container; 11. first inlet groove; 12. second inlet groove; 2. sealing member; 21. plugging portion; 22. exposed portion; 3. fixing member; 31. sealing belt; 32. fixing clip; 4. handle; 5. groove. DETAILED DESCRIPTION

[0034] The present application is further described in detail with reference to the following examples. It should be noted that if no specific conditions are specified in the following examples, the conventional conditions or the conditions recommended by the manufacturer are followed, and the raw materials used in the following examples can be obtained from common commercial sources unless otherwise specified.

[0035] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0036] In this application, "first aspect", "second aspect", "third aspect", etc. are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or quantity, nor can they be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first", "second", "third", etc. only serve the purpose of non-exhaustive enumeration and description, and it should be understood that they do not constitute a closed limitation on quantity.

[0037] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0038] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0039] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as those generally understood by those skilled in the art to which this application belongs. The terms used in this application and in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in this application includes any and all combinations of one or more related listed items. The term "multiple" in this application means at least two, such as two, three, etc., unless otherwise clearly and specifically limited.

[0040] The embodiments of the present application disclose a plant underwater cutting sampling device and a sampling method.

[0041] First, refer to Figure 1 and Figure 2 A plant underwater cutting sampling device comprises a water reservoir 1, a sealing member 2 and a fixing member 3. The side wall of the water reservoir 1 is provided with a first access groove 11 penetrating through the wall thickness thereof along its height. The length of the first access groove 11 is consistent with the height of the water reservoir 1. The bottom wall of the water reservoir 1 is provided with a second access groove 12 penetrating through the bottom wall thickness. One end of the second access groove 12 starts at the center of the bottom wall of the water reservoir 1, and the other end extends toward the first access groove 11 and communicates with the first access groove 11. The first access groove 11 cooperates with the second access groove 12 to allow plant samples to enter the water reservoir 1. The sealing member 2 is detachably connected to the water reservoir 1, and the sealing member 2 is used to block the first access groove 11 and the second access groove 12. The fixing member 3 is used to fix the plant entering the water reservoir 1. It can be understood that the plant sample can be a stem or an organ such as a petiole. In this embodiment, the plant sample is a plant stem.

[0042] It is understandable that the water reservoir 1 can be a cup-shaped hollow structure made of hard materials such as organic glass. In order to facilitate observation, the water reservoir 1 can also be made of transparent organic glass. And the shape of the water reservoir 1 can be any shape such as square, round, etc. In order to facilitate gripping, such as Figure 1 As shown, a handle 4 is detachably or fixedly connected to the side wall of the water storage container 1 .

[0043] When in use, the seal 2 is released from the first and second access grooves 11 and 12, and the plant stems to be sampled are placed upright in the water reservoir 1, and the plant stems are fixed by the fixing member 3, and then the seal 2 is used to block the first and second access grooves 11 and 12, and finally the water reservoir 1 is placed in a solution such as water, the height of which depends on the target cutting position of the plant sample. This allows the subsequent underwater cutting and sampling steps to be carried out directly while maintaining the original state, and directly cutting the sample underwater can allow the plant stem to quickly release the tension of the xylem, and will not introduce other embolic errors, effectively reducing the measurement error caused by sampling; it is applicable to a wide range of plant sample types, is simple to operate, and has a high sampling efficiency.

[0044] In some embodiments, the seal 2 is an elastic strip, combined with Figure 2 and Figure 3 It can be known that the sealing member 2 includes an inserted portion 21 and an exposed portion 22, the inserted portion 21 and the exposed portion 22 are integrally connected, the inserted portion 21 is located in the entire first access groove 11 and the entire second access groove 12, and the exposed portion 22 is in contact with the outer wall of the water storage container 1 on one side close to the inserted portion 21.

[0045] In order to prevent the sealing member 2 from being easily separated from the first access groove 11 and / or the second access groove 12 without human intervention, Figure 4 As shown, the width of the inserted portion 21 at one end away from the exposed portion 22 is greater than the width at one end close to the exposed portion 22. As an example, the longitudinal section of the entire sealing member 2 is "H"-shaped.

[0046] In some embodiments, in order to improve the sealing performance between the seal 2 and the water storage container 1, the edges of the wall on both sides of the first inlet groove 11 and the edges of the wall on both sides of the second inlet groove 12 are fixedly connected with arc-shaped protrusions, such as Figure 4 As shown, a groove 5 for accommodating the arc-shaped protrusion is provided at the corresponding position of the exposed portion 22. After the arc-shaped protrusion fits the groove 5, it can play a good role in fixing the seal 2, thereby improving the sealing performance of the seal 2 and the water storage container 1. The edges of the wall on both sides of the first feed groove 11 and the edges of the wall on both sides of the second feed groove 12 can also be coated with vaseline, which is convenient for installing the wall seal 2 and has a good waterproof effect.

[0047] It should be noted that the fixing member 3 can be a structure such as adhesive tape or a binding tape that can relatively fix the position between the plant sample and the water storage container 1. Figure 2As shown, the fixing member 3 includes a sealing belt 31 and a fixing clamp 32. One end of the sealing belt 31 is bonded to the outer bottom wall of the water reservoir 1, and the other end extends to the center of the bottom wall of the water reservoir 1 and then bends in a direction away from the bottom wall of the water reservoir 1; the end of the sealing member 2 located at the center of the bottom wall of the water reservoir 1 is bent in a direction away from the bottom wall of the water reservoir 1, and the side where the bent portion of the sealing member 2 and the bent portion of the sealing belt 31 are close to each other can fit with the outer wall of the plant stem. The fixing clamp 32 is clamped on the bent portion of the sealing belt 31 and the bent portion of the sealing member 2 at the same time. This fixing method is simple and convenient, and because the fixing clamp 32 is clamped on the sealing belt 31 and the sealing member 2, it does not directly contact the plant, which can reduce damage to the plant stem, thereby helping to promote the accuracy of subsequent plant stem sampling results. It can be understood that after the fixing clamp 32 is clamped on the sealing belt 31 and the sealing member 2, a small amount of sealing glue can be filled in the gap between the bent portion of the sealing belt 31 and the bent portion of the sealing member 2 to achieve a better sealing effect and reduce the sampling error as much as possible.

[0048] It is understandable that when the seal 2 needs to be removed, the fixing clip 32 can be clamped on the handle 4 to reduce the possibility of losing the fixing clip 32. In addition, in some embodiments, in order to further reduce the squeezing of the plant sample by the bent portion of the seal 2, the plugging portion 21 at the bent portion of the seal 2 can be removed, and at this time, the exposed portion 22 can be directly attached to the plant sample.

[0049] It should be noted that the seal 2 and the sealing belt 31 can be made of elastic materials such as latex or silicone. There is good sealing between the water storage container 1 and the plant sample, which will not cause a large amount of water leakage and consumes less water. The entire plant underwater cutting sampling device is low in cost, can be mass-produced and batch-operated by multiple people, and has high sampling efficiency.

[0050] In the second aspect, referring to 1-4, the embodiment of the present application discloses a method for underwater cutting and sampling of plants, which specifically comprises the following steps:

[0051] (1) The plant sample is allowed to pass through the first entry slot 11 and the second entry slot 12 and enter the water storage tank 1, and the first distance below the target cut-off position of the plant sample is located at the center of the bottom wall of the water storage tank 1. It should be noted that the first distance can be 3-5 cm, and as an example, the first distance here is 4 cm.

[0052] (2) Insert the seal 2 into the first access groove 11 and the second access groove 12, and the seal 2 extends to the center of the bottom. The seal 2 can be the elastic strip including the insertion part 21 and the exposed part 22, the width of the insertion part 21 away from the exposed part 22 is greater than the width of the end close to the exposed part 22, and the edges of the wall on both sides of the first access groove 11 and the edges of the wall on both sides of the second access groove 12 are fixedly connected with arc-shaped protrusions, and the corresponding position of the exposed part 22 is provided with a groove 5, and the edges of the wall on both sides of the first access groove 11 and the edges of the wall on both sides of the second access groove 12 are smeared with vaseline. After the insertion part 21 is inserted into the entire first access groove 11 and the entire second access groove 12, the side of the exposed part 22 close to the insertion part 21 fits with the outer wall of the water storage container 1, and the arc-shaped protrusion is located in the groove 5. Vaseline helps the seal 2 to better seal the water storage container 1.

[0053] (3) Fixing the plant sample by the fixing member 3. Specifically, the fixing member 3 may include a sealing belt 31 and a fixing clamp 32. One end of the sealing belt 31 is fixedly connected to the outer bottom wall of the water reservoir 1, and the other end extends to the center of the bottom wall of the water reservoir 1 and then bends away from the bottom wall of the water reservoir 1; the end of the sealing member 2 located at the center of the bottom wall of the water reservoir 1 is bent away from the bottom wall of the water reservoir 1, and the side where the bent portion of the sealing member 2 and the bent portion of the sealing belt 31 are close to each other can fit the plant sample. The fixing clamp 32 is clamped between the bent portion of the sealing belt 31 and the bent portion of the sealing member 2.

[0054] (4) Place the water reservoir 1 in the solution so that the liquid level of the solution is a second distance above the target cutting position; the gap between the exposed stem of the plant and the water reservoir 1 can be filled with sealing glue. It can be understood that the second distance is 3-7 cm. As an example, in this embodiment, the second distance is 5 cm.

[0055] It is understood that the solution can be water, KCl solution, CaCl 2 Solution, etc., in this embodiment, the solution is distilled water.

[0056] (5) Cutting the plant sample at the target cutting position in the solution. Use a blade or pruning shears to cut the stem underwater, or cut several stem segments in succession in the solution from the target cutting position in a direction away from the bottom wall of the water reservoir 1.

[0057] (6) Place a container in the water reservoir 1, and place the cut segments and part of the solution in the water reservoir 1 in the solution through the container, so that the cut segments of the stem are always in the solution. The container can be a beaker or other vessel that can be easily put in and out of the water reservoir 1 to complete the sampling.

[0058] (7) Loosen the fixing clamp 32 and discharge the solution in the water reservoir 1 for next use.

[0059] In the above-mentioned underwater plant cutting sampling method, the plant sample can be a stem or an organ such as a petiole. In this embodiment, the plant sample is a plant stem.

[0060] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A plant underwater cutting sampling device, characterized in that: include, A water reservoir, wherein a first access groove penetrating through the wall thickness of the water reservoir is provided on the side wall of the water reservoir along the height thereof, the length of the first access groove being consistent with the height of the water reservoir, a second access groove penetrating through the wall thickness of the bottom wall is provided on the bottom wall of the water reservoir, one end of the second access groove starts at the center of the bottom wall of the water reservoir, and the other end extends toward the first access groove and is connected with the first access groove, the first access groove cooperates with the second access groove to allow plant samples to enter the water reservoir; A sealing member, the sealing member is detachably connected to the water storage container and is used to seal the first inlet groove and the second inlet groove; A fixing member is used to fix the plant sample entering the water reservoir.

2. The underwater plant cutting sampling device according to claim 1, characterized in that: The sealing member is an elastic strip, which includes an inserted portion and an exposed portion, the inserted portion and the exposed portion are fixedly connected, the inserted portion is located in the entire first access groove and the entire second access groove, and the exposed portion is fitted with the outer wall of the water storage container on one side close to the inserted portion.

3. The underwater plant cutting sampling device according to claim 2, characterized in that: The edges of the vessel wall on both sides of the first feed slot and the edges of the vessel wall on both sides of the second feed slot are fixedly connected with arc-shaped protrusions, and corresponding positions of the exposed portion are provided with grooves for accommodating the arc-shaped protrusions.

4. The underwater plant cutting sampling device according to claim 2, characterized in that: The fixing member includes a sealing belt and a fixing clip, one end of the sealing belt is fixedly connected to the outer bottom wall of the water tank, and the other end extends to the center of the bottom wall of the water tank and then bends in a direction away from the bottom wall of the water tank; one end of the sealing member located at the center of the bottom wall of the water tank is bent in a direction away from the bottom wall of the water tank, and the fixing clip is simultaneously clamped between the bent portion of the sealing belt and the bent portion of the sealing member.

5. The underwater plant cutting sampling device according to claim 4, characterized in that: The materials of the sealing tape and the sealing element are both selected from latex or silicone.

6. The underwater plant cutting sampling device according to claim 4, characterized in that: The side wall of the water storage container is connected with a handle, and the fixing clip is arranged on the handle.

7. The underwater plant cutting sampling device according to claim 1, characterized in that: The water storage container is made of transparent hard material.

8. A method for underwater plant cutting sampling using the sampling device according to any one of claims 1 to 7, characterized in that: The following steps are included: (1) allowing the plant sample to enter the water reservoir through the first and second entry grooves, and allowing a first distance below a target cut-off position of the plant sample to be located at the center of a bottom wall of the water reservoir; (2) inserting a sealing member into the first access groove and the second access groove, wherein the sealing member extends to the center position of the bottom; (3) Fixing the plant specimen by a fixing device; (4) placing the water reservoir into the solution so that the liquid level of the solution is a second distance above the target cut-off position; (5) cutting the plant sample at a target cutting position in the solution; (6) placing a container in the water reservoir, placing the cut segments and part of the solution in the water reservoir in the solution through the container, and the cut segments of the stem are always in the solution.

9. A method for underwater cutting and sampling of plants according to claim 8, characterized in that: The first distance is 3-5 cm; the second distance is 3-7 cm.

10. The method for underwater cutting and sampling of plants according to claim 8, characterized in that: The vessel wall edges on both sides of the first feed groove and the vessel wall edges on both sides of the second feed groove are coated with vaseline.

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