Nutritional composition and pet food beneficial to pet health

Abstract

The present invention discloses a nutritional composition beneficial to pet health and a pet food containing the same. The nutritional composition comprises β-glucan, plasma protein and lactoferrin; wherein the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.275): (1.56-4.68): (0.00095-0.0285). The nutritional composition beneficial to pet health provided by the present invention can improve the intestinal health of pets and enhance digestion ability; it can also enhance the immunity of pets and improve the hair health of pets.

Description

Technical Field

[0001] The present invention relates to the technical field of pet feed, and in particular to a nutritional composition beneficial to pet health and pet food containing the nutritional composition. Background Art

[0002] Pets are gaining increasing importance in people's daily lives. How to ensure the healthy growth of pets, provide them with the trace elements they need through daily diet, enhance their immunity and disease resistance, and reduce the incidence of disease are currently topics of great concern to pet owners. However, for pets that often experience various gastrointestinal digestion and absorption problems, the food they feed them often has a significant impact on their condition.

[0003] Currently, pet food and pet nutritional supplements are available on the market. Pet food, as a nutritious food specifically for cats and dogs, is a high-end pet food that lies between human food and traditional livestock feed. Its primary purpose is to provide pets with essential nutrients for life support, growth, and development. Pet nutritional supplements are dietary supplements made from a variety of nutritional additives formulated in specific proportions to meet a pet's nutritional needs for amino acids, vitamins, minerals, trace elements, enzymes, and other nutrients.

[0004] Pet food and nutritional supplements are required to provide essential nutrients for daily activities, have high digestibility and absorption rates, be scientifically formulated, and protect against diseases in cats and dogs. However, currently, there are relatively few nutritional supplements on the market that can comprehensively improve pet health, with relatively simple combinations and limited functionality. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a nutritional composition beneficial to the health of pets, which can improve the intestinal health of pets and enhance their digestive ability; it can also enhance the immunity of pets and improve the hair health of pets.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A first aspect of the present invention provides a nutritional composition beneficial to pet health, comprising β-glucan, plasma protein and lactoferrin; wherein, in the nutritional composition, the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.275): (1.56-4.68): (0.00095-0.0285).

[0008] β-glucan is a polysaccharide composed of glucose molecules linked by β-1,3-glycosidic bonds or β-1,6-glycosidic bonds. It is widely found in plants, fungi, and bacteria. β-glucan can inhibit inflammatory responses, alleviate inflammatory symptoms, and help alleviate the onset of inflammatory diseases. β-glucan also has a blood sugar-lowering effect, possibly by repairing damaged pancreatic B cells and reducing exogenous glucose absorption. It also has antioxidant, intestinal mucosal protective, and lipid-lowering properties. In the skincare field, β-glucan can promote skin cell activity, achieve skincare benefits, and has excellent moisturizing properties and anti-inflammatory activity.

[0009] Plasma protein, the primary solid component of plasma, is widely used in feed. Rich in essential amino acids and easily digestible, plasma protein provides a protein supplement for pets. It also contains immunoglobulins and other immunoactive substances, which can enhance the body's immune function and improve disease resistance. Plasma protein also helps maintain and repair the intestinal barrier, increasing tight junctions between mucosal epithelial cells, improving intestinal barrier function, repairing damaged epithelial cells, and reducing viral infections. Furthermore, plasma protein improves digestion and enhances palatability.

[0010] Lactoferrin is an iron-binding protein found in breast milk and animal dairy products. It contains immune substances that protect the body from infection by harmful microorganisms and significantly enhances the body's resistance to disease. It also has certain antibacterial properties, inhibiting the growth of some bacteria by binding to iron ions, competing for the iron needed for bacterial reproduction. Lactoferrin also promotes the growth of bifidobacteria and lactic acid bacteria, maintaining a healthy intestinal microecological environment.

[0011] During their research, the inventors discovered that when β-glucan, plasma protein, and lactoferrin are used together, they unexpectedly produce a synergistic effect, significantly improving the health of pets, including improving their intestinal health, digestive ability, immunity, and coat health. Furthermore, the inventors discovered that the β-glucan, plasma protein, and lactoferrin components must be combined in a ratio of (0.055-0.275):(1.56-4.68):(0.00095-0.0285) to achieve this synergistic effect. If the ratios are outside this range, the effectiveness of improving pet health is significantly reduced.

[0012] In the nutritional composition of the present invention, the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.275):(1.56-4.68):(0.00095-0.0285), wherein within this ratio range, the value of β-glucan can be 0.055, 0.1, 0.15, 0.2, 0.25, 0.275, etc., the value of plasma protein can be 1.56, 2, 2.5, 3, 3.5, 4, 4.5, 4.68, etc., and the value of lactoferrin can be 0.00095, 0.001, 0.002, 0.004, 0.005, 0.006, 0.008, 0.01, 0.015, 0.02, 0.025, 0.0285, etc.

[0013] In some preferred embodiments of the present invention, the mass ratio of β-glucan, plasma protein, and lactoferrin in the nutritional composition is (0.055-0.165):(1.56-3.12):(0.00095-0.019). Further preferably, the mass ratio of β-glucan, plasma protein, and lactoferrin is 0.1485:2.34:0.00171.

[0014] In the present invention, the nutritional composition beneficial to pet health can be obtained by directly and evenly mixing three raw materials: β-glucan raw material, porcine plasma protein powder, and lactoferrin powder. The β-glucan raw material, porcine plasma protein powder, and lactoferrin powder can all be commercially available. Preferably, the purity of the active ingredient (β-glucan) in the β-glucan raw material is ≥55.0%, the purity of the active ingredient (plasma protein) in the porcine plasma protein powder is ≥78.0%, and the purity of the active ingredient (lactoferrin) in the lactoferrin powder is ≥95.0%.

[0015] The second aspect of the present invention provides the use of the composition in improving the intestinal health of a pet and / or enhancing the immunity of a pet and / or improving the hair health of a pet.

[0016] A third aspect of the present invention provides use of the above nutritional composition in preparing pet food.

[0017] A fourth aspect of the present invention provides a pet food comprising the above-mentioned nutritional composition.

[0018] The nutritional composition of the present invention can be used as a nutritional supplement for pets. It can exist alone in the form of a powder bag. When used, the powder bag is added to the pet food raw material in a certain proportion to obtain pet food. In some embodiments, the amount of the nutritional composition added to the pet food can be 0.1 to 10 wt%, for example, it can be 0.1 wt%, 0.2 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt%, 0.8 wt%, 1.0 wt%, 2.0 wt%, 3.0 wt%, 4.0 wt%, 5.0 wt%, 6.0 wt%, 7.0 wt%, 8.0 wt%, 9.0 wt%, 10.0 wt%, etc. Compared with the pet food raw materials, after adding the nutritional composition, the nutritional components of the pet food are more comprehensive, which can better improve the digestion and immunity of the pet, and improve the intestinal health and hair health of the pet.

[0019] In some preferred embodiments, the content of β-glucan in the pet food is 0.055-0.275wt%, for example, 0.055wt%, 0.1wt%, 0.15wt%, 0.2wt%, 0.25wt%, 0.275wt%, etc.; the content of plasma protein is 1.56-4.68wt%, for example, 1.56wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%. %, 4.5wt%, 4.68wt%, etc.; the content of lactoferrin is 0.00095-0.0285wt%, for example, it can be 0.00095wt%, 0.001wt%, 0.002wt%, 0.004wt%, 0.005wt%, 0.006wt%, 0.008wt%, 0.01wt%, 0.015wt%, 0.02wt%, 0.025wt%, 0.0285wt%, etc. In a preferred embodiment, the pet food contains 0.1485wt% of beta-glucan, 2.34wt% of plasma protein, and 0.00171wt% of lactoferrin; in another preferred embodiment, the pet food contains 0.1485wt% of beta-glucan, 2.34wt% of plasma protein, and 0.019wt% of lactoferrin.

[0020] It should be noted that the aforementioned pet food ingredients can be pet base food (also known as basal food), which contains some of the most basic nutrients required by pets, such as protein, vitamins, minerals, and some oils. For example, in some embodiments, the basal food may include the following components, by mass percentage: crude protein 30.0%-50.0%, crude fat 9.0%-25.0%, crude fiber ≤9%, moisture ≤14%, calcium 0.6%-1.3%, total phosphorus 0.5%-1.2%, and taurine 0.1%-0.2%. This type of basal food can also be purchased commercially.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. In the nutritional composition beneficial to pet health provided by the present invention, the three components of β-glucan, plasma protein and lactoferrin produce a synergistic effect when compounded in a certain proportion, significantly improving the intestinal health of pets, enhancing the digestive ability and immune ability of pets, and improving the hair health of pets.

[0023] 2. The nutritional composition beneficial to pet health provided by the present invention can be applied to pet food and can improve the health of pets. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Provides a standard for grading pet cat hair;

[0025] Figure 2 This is a graph showing the concentration of fecal metabolites in pet cats of the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0026] Figure 3 Figure 2 is a graph showing the concentration of immune factors in pet cat feces of the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0027] Figure 4 This is a pet cat feces scoring chart for the normal control group, model control group, Example 1 group, and comparative example groups 1 to 6;

[0028] Figure 5 This is a pet cat hair grade scoring chart for the normal control group, model control group, Example 1 group, and comparative example groups 1 to 6;

[0029] Figure 6 This is a diagram of the digestibility index of pet cats in the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0030] Figure 7 This is a graph showing the concentrations of serum biochemical indicators of pet cats in the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0031] Figure 8 Figure 2 is a graph showing the concentration of immune factors in the blood of pet cats in the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0032] Figure 9 This is a graph showing the concentration of fecal metabolites in pet cats of the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0033] Figure 10 Figure 2 is a graph showing the concentration of immune factors in feces of pet cats in the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0034] Figure 11 This is a pet cat feces scoring chart for the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0035] Figure 12 This is a pet cat hair grade scoring chart for the normal control group, model control group, Example 1 to 4 groups, and Comparative Example 7 to 8 groups;

[0036] Figure 13 This is a diagram of the digestibility index of pet cats in the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0037] Figure 14 This is a graph showing the concentrations of serum biochemical indicators of pet cats in the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0038] Figure 15 Graph showing the blood immune factor concentrations of pet cats in the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups. DETAILED DESCRIPTION

[0039] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the accompanying drawings and embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making any creative efforts shall fall within the scope of protection of the present invention.

[0040] In the following examples and comparative examples, the purity of the β-glucan raw material was 55%, the purity of the porcine plasma protein powder was 78%, and the purity of the lactoferrin powder was 95%. The base food used was Suligao Let's Stay In cat food with chicken, apples, and lentils. The composition test results are shown in Table 1. All ingredients, except water and fat, are calculated on a dry basis.

[0041] Table 1

[0042]

[0043]

[0044] Example 1

[0045] This embodiment provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.27 parts of β-glucan raw material, 3 parts of porcine plasma protein powder, and 0.0018 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.1485 parts of β-glucan, 2.34 parts of plasma protein, and 0.00171 parts of lactoferrin, respectively.

[0046] 3.2718 g of the nutritional composition powder of this embodiment was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.1485 wt% of β-glucan, 2.34 wt% of plasma protein, and 0.00171 wt% of lactoferrin.

[0047] Example 2

[0048] This embodiment provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.27 parts of β-glucan raw material, 3 parts of porcine plasma protein powder, and 0.02 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.1485 parts of β-glucan, 2.34 parts of plasma protein, and 0.019 parts of lactoferrin, respectively.

[0049] 3.29 g of the nutritional composition powder of this example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.1485 wt% of β-glucan, 2.34 wt% of plasma protein, and 0.019 wt% of lactoferrin.

[0050] Example 3

[0051] This embodiment provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.5 parts of β-glucan raw material, 2 parts of porcine plasma protein powder, and 0.01 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.275 parts of β-glucan, 1.56 parts of plasma protein, and 0.0095 parts of lactoferrin, respectively.

[0052] 2.51 g of the nutritional composition powder of this embodiment was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.275 wt% of β-glucan, 1.56 wt% of plasma protein, and 0.0095 wt% of lactoferrin.

[0053] Example 4

[0054] This embodiment provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.1 parts of β-glucan raw material, 6 parts of porcine plasma protein powder, and 0.03 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.055 parts of β-glucan, 4.68 parts of plasma protein, and 0.0285 parts of lactoferrin, respectively.

[0055] 6.13 g of the nutritional composition powder of this example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.055 wt% of β-glucan, 4.68 wt% of plasma protein, and 0.0285 wt% of lactoferrin.

[0056] Comparative Example 1

[0057] This comparative example provides a finished pet food, which is obtained by adding 0.27g of β-glucan raw material to 99.73g of base food and mixing. The active ingredient content of the finished pet food is: 0.1485wt% of β-glucan.

[0058] Comparative Example 2

[0059] This comparative example provides a finished pet food, which is obtained by adding 3g of pig plasma protein powder to 97g of base food and mixing. The content of active ingredients in the finished pet food is: 2.34wt% of plasma protein.

[0060] Comparative Example 3

[0061] This comparative example provides a finished pet food, which is obtained by adding 0.0018g of lactoferrin powder to 99.9982g of base food and mixing. The content of the active ingredient in the finished pet food is: 0.00171wt% of lactoferrin.

[0062] Comparative Example 4

[0063] This comparative example provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.27 parts of β-glucan raw material with 3 parts of porcine plasma protein powder. The active ingredients β-glucan and plasma protein in the nutritional composition powder packet are 0.1485 parts of β-glucan and 2.34 parts of plasma protein, respectively.

[0064] 3.27 g of the nutritional composition powder of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.1485 wt% of β-glucan and 2.34 wt% of plasma protein.

[0065] Comparative Example 5

[0066] This comparative example provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.27 parts of β-glucan raw material and 0.0018 parts of lactoferrin powder. The active ingredients β-glucan and lactoferrin in the nutritional composition powder packet are 0.1485 parts of β-glucan and 0.00171 parts of lactoferrin, respectively.

[0067] 0.2718 g of the nutritional composition powder of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.1485 wt% of β-glucan and 0.00171 wt% of lactoferrin.

[0068] Comparative Example 6

[0069] This comparative example provides a nutritional composition powder packet beneficial to pet health, comprising a mixture of 3 parts porcine plasma protein powder and 0.0018 parts lactoferrin powder. The active ingredients, plasma protein and lactoferrin, are contained in the nutritional composition powder packet at 2.34 parts plasma protein and 0.00171 parts lactoferrin, respectively.

[0070] 3.0018 g of the nutritional composition powder of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 2.34 wt% of plasma protein and 0.00171 wt% of lactoferrin.

[0071] Comparative Example 7

[0072] This comparative example provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 0.6 parts of β-glucan raw material, 1.5 parts of porcine plasma protein powder, and 0.0008 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.33 parts of β-glucan, 1.17 parts of plasma protein, and 0.00076 parts of lactoferrin, respectively.

[0073] 2.1008 g of the nutritional composition powder of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.33 wt% of β-glucan, 1.17 wt% of plasma protein, and 0.00076 wt% of lactoferrin.

[0074] Comparative Example 8

[0075] This comparative example provides a nutritional composition powder packet beneficial to pet health. The nutritional composition powder packet is prepared by mixing 1.2 parts of β-glucan raw material, 1.5 parts of porcine plasma protein powder, and 0.05 parts of lactoferrin powder. The active ingredients β-glucan, plasma protein, and lactoferrin in the nutritional composition powder packet are 0.66 parts of β-glucan, 1.17 parts of plasma protein, and 0.0475 parts of lactoferrin, respectively.

[0076] 2.75 g of the nutritional composition powder of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The active ingredient contents of the finished pet food were: 0.66 wt% of β-glucan, 1.17 wt% of plasma protein, and 0.0475 wt% of lactoferrin.

[0077] Trials and Tests

[0078] 1. Experimental Methods

[0079] Healthy adult cats (male-to-female ratio 1:1) of similar age and weight were selected and randomly divided into groups of 6 in each group. They were fed in single cages and fed with corresponding pet food. All groups of test cats were provided with a diet that complies with the AAFCO cat food nutrient composition table. The feed intake was formulated according to the nutritional needs of the test cats during the adult maintenance period, and they were free to eat and drink. The adaptation period was 1 week. Except for the normal control group, the cats in the other groups were required to undergo intestinal immune modeling (modeling period of 1 week) and a recovery period of 3 weeks. Feces, blood or hair were collected at specific time points for the following scores or tests.

[0080] Intestinal immunity modeling test: Use enteric pathogens such as Escherichia coli for gavage / feeding to establish an intestinal immunity model. Use Escherichia coli for gavage and feed 5*10 per kilogram of cat body weight. 10 The cats were fed the pathogenic bacteria for 7 consecutive days, and the model was considered successful if diarrhea persisted for 3-4 days in all cats. Following the modeling period, the cats were fed the control diet again at the beginning of the recovery period. Fecal scores were performed daily, and growth data were recorded weekly. Blood and fecal samples were collected.

[0081] 2. Experimental dose

[0082] Normal control group: Suligao Let's Stay In cat food containing chicken, apple & lentil formula;

[0083] Model control group: Suligao Let's Stay In cat food containing chicken, apple & lentil formula;

[0084] Experimental group: Let's Stay In cat food containing chicken, apple & lentil formula + powder packets of the combination of the embodiment / comparative example.

[0085] 3. Experimental content

[0086] 3.1 Stool quality

[0087] Stool scores were performed according to Table 2 and the average value was taken (the closer the score to 3, the better).

[0088] Table 2

[0089]

[0090]

[0091] The diarrhea rate was calculated by the number of diarrhea episodes during the test period: diarrhea rate (%) = 100 × the number of diarrhea episodes during the test period / the total number of fecal score statistics for cats during the test period.

[0092] 3.2 Nutrient Apparent Digestibility

[0093] During week 6, feces were collected for 5 consecutive days, weighed, and stored in a -20°C freezer until analysis. Diet and fecal samples were analyzed for crude protein (GB / T 6432-2018), dry matter (GB / T 6435-2014), crude fat (GB / T 6433-2006), crude fiber (GB / T 6434-2006), crude ash (GB / T 6438-2007), calcium (GB / T 6436-2018), and phosphorus (GB / T 6437-2018). Gross energy (ISO 9831:1998) was measured in diets and feces using an oxygen bomb calorimeter. Nutrient apparent digestibility was calculated using the following formula:

[0094] Apparent digestibility of a nutrient (%) = (nutrient content in the feed - nutrient content in feces) / (nutrient content in the feed) × 100.

[0095] 3.3 Preparation of blood samples and determination of biochemical indicators

[0096] At the end of the study, blood was collected from the forelimb vein of the cats in the experimental and control groups. A portion of the blood was analyzed for routine blood analysis, and the remaining serum was separated for blood biochemistry analysis. Blood biochemistry parameters included albumin (ALB), total protein (TP), total bilirubin (TB), inorganic phosphate (PHOS), calcium (Ca), globulin (GLOB), alanine aminotransferase (ALT), and aspartate aminotransferase (AST).

[0097] Among the above-mentioned blood biochemical indicators, total protein is better between 54.00 and 89.00, albumin is better between 22.00 and 45.00, and globulin is better between 15.00 and 57.00; alanine aminotransferase is better between 8.2 and 100, and higher levels will cause liver damage; aspartate aminotransferase is better between 9.2 and 39.5, and higher levels will cause liver damage.

[0098] 3.4 Hair health

[0099] On the 42nd day of the experiment, the hair of each cat's back was collected and the hair score (HS) was performed using the method described by Rees et al. under the same lighting conditions and on the same observation platform. The scoring criteria are shown in Table 3 and Figure 1 .

[0100] Table 3

[0101]

[0102]

[0103] Among them, 1≤HS≤2 is dry; 2<HS<4 is ideal; 4≤HS≤5 is greasy.

[0104] 3.5 Metabolite Detection

[0105] Serum and fecal samples were collected from animals to determine the concentrations of metabolites in feces, including acetic acid, propionic acid, butyric acid, valeric acid, isovaleric acid, and isobutyric acid.

[0106] 3.6 Detection of inflammatory and immune factors

[0107] Enzyme-linked immunosorbent assay (ELISA) was used to detect immune indicators such as tumor necrosis factor-α (TNF-α), gamma-interferon (IFN-γ), interleukins IL-1β, IL-6, IL-8, IL-10, immunoglobulins IgA, IgG, and IgM in serum, and immunoglobulins IgA, IgG, and IgM in feces.

[0108] 3.7 Intestinal health index testing

[0109] After the adaptation period, intestinal immune modeling was performed: cats in the experimental group and the model control group were gavaged with bacterial culture fluid (bacteria: intestinal pathogenic Escherichia coli), and the individual infection and recovery conditions during the gavage period, fecal scores, and metabolites were observed and recorded.

[0110] 3.8 Data Processing and Analysis

[0111] Independent sample t-tests were performed using SPSS 26.0 software. Results are expressed as mean ± standard error. Letters indicate significant differences; different letters indicate significant differences between groups. Single letters indicate strongly significant differences (p < 0.01); double letters indicate weakly significant differences (p < 0.05).

[0112] Test Example 1: Effects of Nutritional Composition Ingredients on the Health of Pet Cats

[0113] The effects of the nutritional composition ingredients on the health of pet cats are shown in Tables 4 and 5.

[0114] Table 4

[0115]

[0116]

[0117]

[0118] Table 5

[0119]

[0120]

[0121] From Table 4-5 and Figure 2-8 The results show that compared with the normal control group, the pet cats in the model control group showed deterioration in many indicators such as digestibility index, fecal quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health. This shows that after intestinal immune modeling, the health status of pet cats has declined significantly.

[0122] See Table 4 and Figure 2-8 In Comparative Examples 1-3, when β-glucan, plasma protein, or lactoferrin were used alone in the nutritional composition, the health of the pet cats could be improved to a certain extent. Compared with the model control group, the pet cats in Comparative Examples 1-3 showed improvements in multiple indicators such as digestibility, fecal quality, fecal immune factors, fecal metabolites, blood immune and inflammatory factors, serum biochemistry, and hair health. However, most indicators were still inferior to those of the pet cats in the normal control group.

[0123] In Comparative Examples 4 to 6, when two of β-glucan, plasma protein and lactoferrin are used in the nutritional composition, the health of the pet cat can be improved to a certain extent, and the effect is better than that of the nutritional composition of Comparative Examples 1 to 3. Figure 2-8As can be seen, the cats in Comparative Examples 4-6 showed significant improvements in digestibility, stool quality, fecal immune factors, fecal metabolites, blood immune and inflammatory factors, serum biochemistry, and hair health compared to the model control group. They also performed better than the cats in Comparative Examples 1-3, but were still inferior to the cats in the normal control group in most indicators. This suggests that the combined use of β-glucan, plasma protein, and lactoferrin can better improve the health of cats than using either alone.

[0124] In Example 1, when the nutritional composition contains β-glucan, plasma protein and lactoferrin at the same time, the results show that the health of the pet cat can be significantly improved, and the effect is better than that of the nutritional compositions of Comparative Examples 1 to 6. Figure 2-8 As can be seen, the pet cats in Example 1 showed significant improvements in digestibility, stool quality, fecal immune factors, fecal metabolites, blood immune and inflammatory factors, serum biochemistry, and hair health compared to the model control group. They also showed significant improvements compared to Comparative Examples 1 to 6, and were superior to the pet cats in the normal control group in most indicators. This indicates that when the nutritional composition contains β-glucan, plasma protein, and lactoferrin at the same time, the three active components can synergize and enhance each other, thereby better improving the intestinal health, immune capacity, and hair health of pets.

[0125] Experimental Example 2: Effect of the Dosage of the Nutritional Composition on the Health of Pet Cats

[0126] The effects of the dosage of the nutritional composition on the health of pet cats are shown in Tables 6-7.

[0127] Table 6

[0128]

[0129]

[0130] Table 7

[0131]

[0132]

[0133] Please refer to Table 6-7 and Figure 9-15The nutritional compositions of Examples 1 to 4 simultaneously contain three active ingredients: β-glucan, plasma protein, and lactoferrin, and their ratios are within the range of (0.055-0.275): (1.56-4.68): (0.00095-0.0285). The results show that the pet cats of Examples 1 to 4 showed significant improvements in digestibility, stool quality, fecal immune factors, fecal metabolites, blood immune and inflammatory factors, serum biochemistry, and hair health compared to the model control group, and were comparable to or better than the normal control group.

[0134] See Table 6 and Figure 9-15 The nutritional compositions of Comparative Examples 7 and 8 simultaneously contain three active ingredients: β-glucan, plasma protein, and lactoferrin, but their ratios are not within the range of (0.055-0.275): (1.56-4.68): (0.00095-0.0285). The results show that the pet cats in Comparative Examples 7 and 8 have improved in digestibility, stool quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry, and hair health compared to the model control group, but are still lower than those in the normal control group, and there is still a certain gap compared to the pet cats in Example 1. This shows that the three active ingredients of β-glucan, plasma protein and lactoferrin in the nutritional composition can better exert their synergistic effect when they are compounded in the ratio of (0.055~0.275):(1.56~4.68):(0.00095~0.0285), thereby comprehensively improving the intestinal health of pets, enhancing immunity, and improving hair health.

[0135] In summary, the composition provided by the present invention can comprehensively improve the health of pets. Specifically, it can improve the intestinal health of pets, reduce the diarrhea rate, enhance the immunity of pets, and improve the hair health of pets.

[0136] The above embodiments are merely preferred embodiments for the purpose of fully illustrating the present invention, and the scope of protection of the present invention is not limited thereto. Equivalent substitutions or modifications made by those skilled in the art based on the present invention are within the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

Claims

1. A nutritional composition beneficial to pet health, characterized in that: The nutritional composition comprises β-glucan, porcine plasma protein and lactoferrin; wherein the mass ratio of β-glucan, porcine plasma protein and lactoferrin is (0.055-0.275): (1.56-4.68): (0.00095-0.0285).

2. A nutritional composition beneficial to pet health according to claim 1, characterized in that: In the nutritional composition, the mass ratio of β-glucan, porcine plasma protein and lactoferrin is (0.055-0.165): (1.56-3.12): (0.00095-0.019).

3. A nutritional composition beneficial to pet health according to claim 1, characterized in that: In the nutritional composition, the mass ratio of β-glucan, porcine plasma protein and lactoferrin is 0.1485:2.34:0.00171.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of a product for improving the intestinal health of pets.

5. Use of the composition according to any one of claims 1 to 3 in the preparation of a product for improving the immunity of pets.

6. Use of the composition according to any one of claims 1 to 3 in the preparation of a product for improving pet hair health.

7. Use of the composition according to any one of claims 1 to 3 in preparing pet food.

8. A pet food, characterized in that: The pet food comprises the nutritional composition according to any one of claims 1 to 3.

9. The pet food according to claim 8, characterized in that: The nutritional composition is added to the pet food in an amount of 0.1 to 10 wt %.

10. The pet food according to claim 8, characterized in that: In the pet food, the content of β-glucan is 0.055-0.275 wt %, the content of porcine plasma protein is 1.56-4.68 wt %, and the content of lactoferrin is 0.00095-0.0285 wt %.

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