Probiotic composition for loosening bowel to relieve constipation and application thereof

Abstract

The invention belongs to the technical field of food, and particularly relates to a probiotic composition capable of relaxing bowel and application, the probiotic composition capable of relaxing bowel comprises compound lactic acid bacteria powder, compound prebiotics and aronia melanocarpa fruit powder, the compound lactic acid bacteria powder comprises lactococcus lactis WHH2078, lactobacillus plantarum WHH581 and lactobacillus reuteri WHH1689, the lactococcus lactis WHH2078 can improve the content of 5-HT in intestinal tracts and accelerate intestinal tract movement by adjusting metabolism of the 5-HT, and the lactococcus lactis WHH2078 is cooperatively matched with other components capable of relaxing bowel, so that constipation is relieved, and meanwhile, no side effect exists.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and particularly relates to a probiotic composition for moistening intestines and facilitating bowel movements and an application thereof. Background Art

[0002] Chronic constipation is a common gastrointestinal disease. Due to changes in dietary structure, pace of life, and complex social and psychological influences, the incidence of chronic constipation has increased year by year, and the current global incidence rate is 14-30%. Chronic constipation has a serious negative impact on people's quality of life and brings a huge economic burden to society.

[0003] The main causes of chronic constipation are colonic sensory and motor dysfunction and changes in intestinal flora. Chronic constipation is the earliest manifestation of gastrointestinal hypomotility, which is characterized by a significantly prolonged intestinal transit time. Slower colon transit speed prolongs the retention time of intestinal contents and increases the reabsorption of water and electrolytes, resulting in reduced stool volume and harder stool. At present, increasing intestinal motility and water secretion is an important direction for the development of constipation drugs. Among them, osmotic laxatives, such as polyethylene glycol, and stimulant laxatives, such as bisacodyl, can quickly promote the secretion of water into the intestine, increase the water content of stool to soften it, stimulate intestinal peristalsis, and have the effect of fecal excretion, but the side effects are abdominal distension, abdominal pain, and diarrhea.

[0004] 5-HT is a mediator involved in regulating multiple physiological functions of the gastrointestinal tract and plays an important role in regulating intestinal motility and intestinal secretion. 5-HT can induce intestinal smooth muscle contraction by activating 5-HT receptors and sending signals through the intestinal myenteric plexus. Prucalopride is a 5-HT receptor agonist that has the activity of promoting colonic peristalsis. It can increase intestinal muscle contraction and the frequency of bowel movements in patients with chronic constipation, but it is expensive. In addition, the study found that compared with the healthy group, the number of bifidobacteria and lactobacilli in patients with chronic constipation was reduced. Short-chain fatty acids produced by intestinal microbial metabolism can also affect intestinal peristalsis. Probiotics can regulate the intestinal flora of patients with constipation, improve intestinal peristalsis, relieve constipation, and are safe and have no side effects.

[0005] Compared with drugs, dietary therapy has a higher safety factor and can be taken for a long time, but the regulatory effect is weak and the onset time is slow. Currently, foods based on probiotics for intestinal lubrication and laxatives on the market mainly focus on regulating intestinal flora, and there is no invention patent that focuses on probiotics regulating 5-HT metabolism, increasing the content of 5-HT in the intestine, and accelerating intestinal peristalsis to achieve the effect of relieving constipation. Summary of the invention

[0006] The present invention aims at the fact that there is no probiotic-related product in the prior art that can regulate 5-HT metabolism, thereby increasing the 5-HT content in the intestine and accelerating intestinal peristalsis to achieve the effect of relieving constipation. The present invention provides a probiotic composition and application for moisturizing the intestine and relieving constipation. The present method provides a method that can increase the 5-HT concentration in the intestine, increase intestinal peristalsis, and increase the abundance of bifidobacteria in the intestine, and at the same time compound a variety of prebiotics, which has a strong laxative effect as a whole, and also has a positive therapeutic effect on patients with severe constipation.

[0007] To achieve the above object, the present invention adopts the following technical solutions: A probiotic composition for moistening the intestine and relieving constipation is characterized in that it comprises: compound lactic acid bacteria powder, compound prebiotics and aronia melanocarpa fruit powder; the compound lactic acid bacteria powder comprises: Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689.

[0008] The present invention is composed of several specific probiotic strains to form a composite lactic acid bacteria powder, including Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689. Among them, Lactococcus lactis WHH2078 can promote the secretion of intestinal 5-HT precursors, thereby increasing intestinal peristalsis; composite laxative Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689 that improves the abundance of intestinal bifidobacteria regulate intestinal function as a whole and enhance intestinal motility. On the basis of composite lactic acid bacteria, multiple prebiotics are compounded to provide nutrients for the proliferation of probiotics; black fruit glandular rib aronia fruit powder provides product flavor. The components synergistically moisturize the intestines and laxative, all of which have been verified by human food trial experiments, and the components synergistically promote laxatives.

[0009] Furthermore, the Lactococcus lactis WHH2078 was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on February 25, 2021, with a deposit number of CGMCC No. 21831. Previous studies have found that in in vitro R1N14B cell experiments, Lactococcus lactis WHH2078 can promote the expression of 5-hydroxytryptophan (5-HTP), a precursor of 5-HT, in cells; mice were gavaged with Lactococcus lactis WHH2078, which can increase the content of 5-HTP in the intestine and serum. It is speculated that Lactococcus lactis WHH2078 can promote the conversion of 5-HTP in the intestine to 5-HT, thereby promoting intestinal muscle contraction and improving intestinal peristalsis.

[0010] Furthermore, Lactobacillus plantarum WHH581 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 18, 2016, with the deposit number of CGMCC No. 13121. Previous studies have found that Lactobacillus plantarum WHH581 can promote defecation in constipation.

[0011] Furthermore, Lactobacillus reuteri WHH1689 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 18, 2016, with the deposit number of CGMCC No. 13122. Previous studies have found that oral administration of Lactobacillus reuteri WHH1689 to mice with DSS-induced enteritis can significantly increase the abundance of bifidobacteria in the intestinal flora.

[0012] Furthermore, the components are calculated by weight as follows: 1 to 10 parts of compound lactic acid bacteria powder, 40 to 140 parts of compound prebiotics, and 1 to 10 parts of aronia nigra fruit powder.

[0013] Preferably, the added amount of Aronia nigra fruit powder is 3 to 8 parts by weight.

[0014] Furthermore, the weight ratio of the components of the composite lactic acid bacteria powder is: Lactococcus lactis WHH2078: Lactobacillus plantarum WHH581: Lactobacillus reuteri WHH1689=2:0.9-1.1:0.9-1.1.

[0015] Furthermore, the number of viable bacteria in the composite lactic acid bacteria powder is 300 billion to 500 billion CFU / g.

[0016] Furthermore, the complex prebiotics include stachyose, xylo-oligosaccharides and lactitol.

[0017] Furthermore, the amount of stachyose added is 10 to 70 parts by weight.

[0018] Preferably, the amount of stachyose added is 30 to 60 parts by weight.

[0019] Furthermore, the added amount of xylo-oligosaccharide is 10 to 30 parts by weight.

[0020] Preferably, the amount of xylo-oligosaccharide added is 10 to 20 parts by weight.

[0021] Furthermore, the added amount of lactitol is 20 to 40 parts by weight.

[0022] Preferably, the amount of lactitol added is 20 to 30 parts by weight.

[0023] Furthermore, the use of the bowel-moistening and laxative probiotic composition in the preparation of a product having the bowel-moistening and laxative effect.

[0024] Furthermore, the product is in the form of powder, granules, tablets or capsules.

[0025] Compared with the prior art, the present invention has the following beneficial effects: 1. Adding Lactococcus lactis WHH2078 to the compound lactic acid bacteria powder can significantly promote the secretion of intestinal 5-HT precursors, thereby increasing intestinal peristalsis.

[0026] 2. The addition of Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689 can not only promote bowel movements, but also increase the abundance of intestinal bifidobacteria, thereby regulating the overall intestinal function and improving intestinal motility.

[0027] 3. By using a combination of three prebiotics, including stachyose, xylo-oligosaccharides and lactitol, on the basis of compound lactic acid bacteria, they provide nutrients for the proliferation of probiotics and ensure the normal function of the bacteria.

[0028] 4. The invention has an excellent formula and definite efficacy. The compound lactic acid bacteria powder regulates intestinal function and improves intestinal motility; the compound prebiotics provide nutrients for the proliferation of probiotics; and the black fruit glandular rib aronia fruit powder provides product flavor. The components synergistically moisturize the intestines and promote defecation. It has been verified by human food trial experiments that the components synergistically promote defecation. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with specific implementation methods.

[0030] The inclusion criteria for subjects in all embodiments and comparative examples are: A) 40-80 years old; B) constipation for more than 5 years, with bowel movements ≤ 2 times per week; C) Voluntarily participate in the trial, can consume the product as required, and have strong compliance.

[0031] Example 1 (1) Mixing A probiotic composition for moistening the intestine and relieving constipation, the raw materials comprising: composite lactic acid bacteria powder (Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689), stachyose, oligoxylose, lactitol and aronia oleracea fruit powder. The above components are mixed by weight to obtain a primary product by evenly mixing 2.5 parts of Lactococcus lactis WHH2078, 1.25 parts of Lactobacillus plantarum WHH581, 1.25 parts of Lactobacillus reuteri WHH1689, 50 parts of stachyose, 10 parts of oligoxylose, 30 parts of lactitol and 5 parts of aronia oleracea fruit powder.

[0032] (2) Preparation The primary product obtained in step (1) is made into powder to obtain the product.

[0033] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0034] Comparative Example 1 The difference between this comparative example and Example 1 is that Lactococcus lactis WHH2078 is not added in step (1), and the rest of the process is the same as that of Example 1. The specific process is as follows: (1) Mixing A probiotic composition for moistening the intestine and relieving constipation is prepared by uniformly mixing, by weight, 1.25 parts of Lactobacillus plantarum WHH581, 1.25 parts of Lactobacillus reuteri WHH1689, 50 parts of stachyose, 10 parts of xylo-oligosaccharides, 30 parts of lactitol and 5 parts of Aronia nigra fruit powder to obtain a primary product.

[0035] (2) Preparation The primary product obtained in step (1) is made into powder to obtain the product.

[0036] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0037] Table 1 Effect of Lactococcus lactis WHH2078 on intestinal moistening and laxative As shown in Table 1, the defecation frequency data obtained by comparing Example 1 with Comparative Example 1 show that when Lactococcus lactis WHH2078 is added, the defecation effect is significantly enhanced. In the first cycle of 7 days of consumption, Example 1 shows a very significant laxative effect (p<0.001), but Comparative Example 1 only has a trend of increasing defecation, and there is no significant difference. Comparative Example 1 will not show a significant laxative effect until the second cycle of consumption (14 days) (p<0.01), and the effect is significantly weaker than that of Example 1. The reason is presumed to be that Lactococcus lactis WHH2078 can promote the secretion of 5-HTP, the precursor of intestinal 5-HT, thereby increasing the concentration of 5-HT in the intestine and increasing intestinal peristalsis. Adding Lactococcus lactis WHH2078 within a certain range can moisten the intestine and relieve constipation.

[0038] Comparative Example 2 The difference between this comparative example and Example 1 is that the composite lactic acid bacteria added in step (1) is Danisco composite lactic acid bacteria powder (including thermophilic Streptococcus and Bulgarian Lactobacillus), and the rest of the process is the same as Example 1, and the specific process is as follows: (1) Mixing A probiotic composition for moistening the intestine and relieving constipation is prepared by uniformly mixing 5 parts of Danisco compound lactic acid bacteria powder (Streptococcus thermophilus and Lactobacillus bulgaricus), 50 parts of stachyose, 10 parts of xylo-oligosaccharides, 30 parts of lactitol and 5 parts of Aronia nigra fruit powder by weight to obtain a primary product.

[0039] (2) Preparation The primary product obtained in step (1) is made into powder to obtain the product.

[0040] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0041] Table 2 Comparison of the effects of the composite lactic acid bacteria powder used in the present invention and the composite lactic acid bacteria powder of Danisco on the intestinal moistening and laxative effect on the human body As shown in Table 2, the defecation frequency data obtained by comparing Example 1 with Comparative Example 2. After consuming for 14 days, the defecation frequency of Comparative Example 2 did not increase significantly, but only showed a slight trend. After adding the composite lactic acid bacteria powder disclosed in the present invention, the defecation frequency of the test sample was significantly higher than that of the sample after adding the composite lactic acid bacteria powder of Danisco, indicating that the specific probiotic combination in the present invention has a good effect on enhancing bowel movement and laxative effect, which is not achievable by ordinary lactic acid bacteria combinations.

[0042] Comparative Example 3 The difference between this comparative example and Example 1 is that there is no prebiotic (stachyose, xylo-oligosaccharide, lactitol) in step (1), and the rest of the process is the same as that of Example 1. The specific process is as follows: (1) Mixing A probiotic composition for moistening the intestine and relieving constipation is prepared by uniformly mixing, by weight, 2.5 parts of Lactococcus lactis WHH2078, 1.25 parts of Lactobacillus plantarum WHH581, 1.25 parts of Lactobacillus reuteri WHH1689 and 5 parts of Aronia nigra fruit powder to obtain a primary product.

[0043] (2) Preparation The primary product obtained in step (1) is made into powder to obtain the product.

[0044] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0045] Table 3 Effect of adding prebiotics on the laxative effect As shown in Table 3, by comparing the defecation frequency data obtained in Example 1 and Comparative Example 3, it is found that after the addition of the composite prebiotics, the defecation frequency is significantly higher than the defecation frequency without the addition of prebiotics, and it is speculated that the reason is that prebiotics, as dietary fiber, selectively stimulate the growth and activity of one or more probiotics, thereby assisting in enhancing the technical effect of moisturizing the intestine and relieving constipation.

[0046] Example 2 (1) Mixing A probiotic composition for moistening the intestine and relieving constipation, the raw materials comprising: composite lactic acid bacteria powder (Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689), stachyose, oligoxylose, lactitol and aronia oleracea fruit powder. The above components are mixed by weight to obtain a primary product by evenly mixing 5 parts of Lactococcus lactis WHH2078, 1.25 parts of Lactobacillus plantarum WHH581, 1.25 parts of Lactobacillus reuteri WHH1689, 30 parts of stachyose, 30 parts of oligoxylose, 20 parts of lactitol and 10 parts of aronia oleracea fruit powder.

[0047] (2) Preparation The primary product obtained in step (1) is made into granules to obtain the product.

[0048] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0049] Example 3 (1) Mixing A probiotic composition for moistening the intestine and relieving constipation, the raw materials comprising: composite lactic acid bacteria powder (Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689), stachyose, xylo-oligosaccharide, lactitol and black-line tear aronia fruit powder. The above components are mixed by weight to obtain a primary product by evenly mixing 1.5 parts of Lactococcus lactis WHH2078, 0.75 parts of Lactobacillus plantarum WHH581, 0.75 parts of Lactobacillus reuteri WHH1689, 35 parts of stachyose, 20 parts of xylo-oligosaccharide, 40 parts of lactitol and 1 part of black-line tear aronia fruit powder.

[0050] (2) Preparation The primary product obtained in step (1) is made into capsules to obtain the product.

[0051] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0052] Example 4 (1) Mixing A probiotic composition for moistening the intestine and relieving constipation, the raw materials comprising: composite lactic acid bacteria powder (Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689), stachyose, oligoxylose, lactitol and aronia oleracea fruit powder. The above components are mixed uniformly by weight: 2 parts of Lactococcus lactis WHH2078, 1 part of Lactobacillus plantarum WHH581, 1 part of Lactobacillus reuteri WHH1689, 60 parts of stachyose, 10 parts of oligoxylose, 20 parts of lactitol and 5 parts of aronia oleracea fruit powder to obtain a primary product.

[0053] (2) Preparation The primary product obtained in step (1) is made into tablets to obtain the product.

[0054] (3) Detection Patients with severe constipation were recruited, and the product obtained in step (2) was used for a human food test on the patients. The test period was 7 days, and the samples were consumed for 14 days. During this period, the bowel movements were observed and recorded every day, and the number of bowel movements during the test period was counted.

[0055] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A probiotic composition for moistening the intestine and relieving constipation, characterized in that: include: Compound lactic acid bacteria powder, compound prebiotics and Aronia nigra fruit powder; The composite lactic acid bacteria powder comprises: Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689, and the weight ratio of Lactococcus lactis WHH2078, Lactobacillus plantarum WHH581 and Lactobacillus reuteri WHH1689 is 2:0.9~1.1:0.9~1.

1.

2. A laxative probiotic composition according to claim 1, characterized in that: The components are calculated by weight: 1~10 parts of compound lactic acid bacteria powder, 80~100 servings of compound prebiotics, 1-10 parts of Aronia nigra fruit powder.

3. A laxative probiotic composition according to claim 2, characterized in that: The added amount of the Aronia nigra fruit powder is 3 to 8 parts by weight.

4. A laxative probiotic composition according to claim 1 or 2, characterized in that: The viable bacteria count of the composite lactic acid bacteria powder is 300 billion to 500 billion CFU / g.

5. A laxative probiotic composition according to claim 1 or 2, characterized in that: The complex prebiotics include stachyose, xylo-oligosaccharide and lactitol.

6. The intestinal laxative probiotic composition according to claim 5, characterized in that: The amount of stachyose added is 10 to 70 parts by weight.

7. The intestinal laxative probiotic composition according to claim 5, characterized in that: The added amount of the xylooligosaccharide is 10 to 30 parts by weight.

8. The intestinal laxative probiotic composition according to claim 5, characterized in that: The added amount of the lactitol is 20 to 40 parts by weight.

9. Use of the bowel-moistening and laxative probiotic composition according to any one of claims 1 to 8 in the preparation of a product having a bowel-moistening and laxative effect.

10. The use according to claim 9, characterized in that: The product is in the form of powder, granules, tablets or capsules.

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