Urine sample automatic collection and pretreatment system

Abstract

The invention discloses a urine sample automatic collection and pretreatment system, and relates to the technical field of sample automatic collection systems.The urine sample automatic collection and pretreatment system comprises a sample automatic collection module and a pretreatment system.The sample collection module is used for collecting urine samples and ensuring freshness and representativeness of the samples; the pretreatment system comprises a sample collection and storage module, and in the urine collection process of the system, urine firstly flows into a drainage tank through a urinal and is finally discharged into a sewer pipe; in the process, the system monitors the flow of the second electromagnetic valve through the flow meter, once the flow reaches a preset flow value, the second electromagnetic valve is automatically closed, and meanwhile, the first electromagnetic valve is opened, so that urine in the drainage tank flows into the collecting pipe through the first electromagnetic valve; then, the urine is conveyed to a pretreatment system through a three-way valve and a conveying pipe; by means of the design, pollution of urine in the front-end collection process is effectively avoided, the deviation of the number of bacteria in the urine is reduced, and the accuracy of a detection result is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of automatic sample collection systems, and in particular to an automatic urine sample collection and pretreatment system. Background Art

[0002] Changes in the types and quantities of proteins in urine carry a variety of information about the occurrence, development and prognosis of urinary system diseases, and urine proteomics is one of the most effective methods to interpret the information carried by urine proteins. In the field of clinical applications, multi-center and large samples are often required for research and analysis in order to obtain more accurate results. After searching, the Chinese patent announcement number CN114354333A discloses a urine sample pretreatment method, preservation method, automated processing system and detection method. Although the processing system greatly reduces people's labor intensity, it is conducive to speeding up the processing efficiency of urine sample processing, meets the needs of high-throughput and automated proteomics pretreatment, and adapts to the reproducibility and throughput of current clinical needs, the system cannot collect urine in segments when collecting urine. If the front urine is collected, it may contain bacteria from the urethral orifice, resulting in deviations in the indicators of bacterial count in urine routine.

[0003] To this end, the present invention proposes an automatic urine sample collection and pretreatment system, which can collect urine in sections and avoid collecting the front end of urine, thereby improving the accuracy of sample collection. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides an automatic urine sample collection and pretreatment system, which solves the problem that the existing system proposed in the background technology is unable to collect urine in segments when collecting urine, which easily leads to deviations in indicators related to bacterial counts in urine routine tests.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a urine sample automatic collection and pretreatment system, including a sample automatic collection module and a pretreatment system, the sample collection module is used to collect urine samples to ensure the freshness and representativeness of the samples; the pretreatment system includes:

[0006] A sample collection and storage module, which is responsible for the collection and storage of urine samples, ensuring that the samples can be stored safely and hygienically after collection until the next step of processing;

[0007] A sample pre-processing reaction module, which involves chemical treatment and biological reaction of urine samples, including adding treatment fluids and separating proteins to prepare the samples for further analysis;

[0008] A sample concentration and separation module, which is responsible for concentrating and separating specific components in a urine sample by reducing the sample volume and separating solid particles or cells to facilitate subsequent analysis and detection;

[0009] The sample analysis storage module is used for final sample analysis and result storage, for molecular biology analysis, and to ensure the stability of the sample during the analysis and the reliability of the results.

[0010] Preferably, the sample collection and storage module includes a urine sample storage unit and a sample suction unit;

[0011] The urine sample storage unit is used to store the collected urine samples, ensuring that the samples are stored under appropriate conditions to avoid contamination and deterioration;

[0012] The sample suction unit comprises a discharge gun device for accurately sucking urine samples from the storage unit in preparation for the next step of processing.

[0013] Preferably, the sample pretreatment reaction module comprises a treatment liquid supply unit, a PVDF filter plate supply unit, a protein collection unit, an enzyme storage unit, a cleavage reaction container supply unit and a shaking table;

[0014] The processing liquid supply unit provides lysing liquid, reducing agent, alkylating agent and chemical reagent for pretreatment of urine samples, such as cell lysis and chemical modification;

[0015] The PVDF filter plate supply unit provides a PVDF filter plate for adsorbing proteins in urine samples for subsequent separation and analysis;

[0016] The protein collection unit collects the filtered or adsorbed proteins to prepare for subsequent protein analysis;

[0017] The enzyme storage unit stores enzymes used for urine sample processing, such as enzymes used to catalyze specific chemical reactions;

[0018] The lysis reaction container supply unit provides a container for holding urine samples and lysis solution to perform cell lysis reaction;

[0019] The shaker is used to mix the urine sample and the processing solution to ensure that the reaction proceeds evenly.

[0020] Preferably, the sample concentration and separation module includes a concentrator and a centrifugal module;

[0021] The concentrator is used to reduce the volume of a urine sample and increase the concentration of a target analyte for more accurate analysis;

[0022] The centrifugal module separates different components in the urine sample, such as cells and sediments, by centrifugal force.

[0023] Preferably, the sample analysis storage module includes a PCR plate and a dilution module;

[0024] The PCR plate is used to store and process pre-treated urine samples and is commonly used in molecular biology techniques such as PCR;

[0025] The dilution module dilutes the urine sample according to the analysis requirements to adjust the sample concentration to a level suitable for analysis.

[0026] Preferably, the sample concentration and separation module also includes a liquid cooling technology unit for providing efficient thermal management during the concentration and separation of urine samples; the liquid cooling technology unit utilizes liquid cooling technology to reduce the heat generated during sample processing to maintain the stability and activity of the sample.

[0027] Preferably, the sample collection module includes a control box, which is used for overall control of the system. The urinal is installed on the wall, a drain groove is installed at the bottom of the urinal, and the drain groove is connected to the hole groove at the bottom of the urinal. A first solenoid valve and a second solenoid valve are arranged at one end of the drain groove, and one end of the first solenoid valve and the second solenoid valve are both connected to the inside of the drain groove. The output end of the second solenoid valve is connected to the input end of the flow meter, and the output end of the flow meter is connected to the sewer pipe through a drain pipe;

[0028] The first solenoid valve is connected to one end of the collecting pipe, and the other end of the collecting pipe is connected to the three-way valve. A drain port is arranged at the bottom of the three-way valve, and a tray is arranged below the drain port. The surface of the tray is provided with filter holes connected to the sewer pipe. One side of the three-way valve is connected to the pretreatment system through the delivery pipe. The flow of the second solenoid valve is detected by the flow meter. When the specified flow value is reached, the second solenoid valve is closed and the first solenoid valve is opened, so that the urine in the drain trough can be discharged into the collecting pipe through the first solenoid valve, and then transported to the pretreatment system through the three-way valve and the delivery pipe, which is beneficial to avoid the front end of urine collection, thereby helping to reduce the deviation of the number of bacteria in urine and improve the accuracy of the detection result.

[0029] Preferably, the top of the urinal is connected to a water valve, and the water valve is connected to the inside of a water tank through a pipe. The water tank is located above the urinal and is filled with disinfectant. A water inlet is installed on one side of the top of the water tank. By opening the water valve, the disinfectant in the water tank can be discharged into the urinal for cleaning, and then the disinfectant will flow through the drain groove and the second solenoid valve for disinfection and flushing.

[0030] The present invention provides a urine sample automatic collection and pretreatment system. It has the following beneficial effects:

[0031] (1) An automatic urine sample collection and pretreatment system. During the urine collection process, the urine first flows into the drain through the urinal, then flows into the drain pipe through the second solenoid valve, and finally flows into the sewer pipe. During this process, the system uses a flow meter to monitor the flow of the second solenoid valve. Once the preset flow value is reached, the second solenoid valve will automatically close, and the first solenoid valve will open at the same time, allowing the urine in the drain to flow into the collection pipe through the first solenoid valve. Subsequently, the urine is transported to the pretreatment system through the three-way valve and the delivery pipe. This design effectively avoids contamination of urine during the front-end collection process, thereby reducing the deviation of the number of bacteria in urine and improving the accuracy of the test results.

[0032] (2) An automatic urine sample collection and pretreatment system, which operates a three-way valve to open the drain port at the bottom of the three-way valve, and then opens the water valve to allow the disinfectant in the water tank to flow into the urinal for cleaning and disinfection; the disinfectant then flows through the drain trough and the second solenoid valve, during which the flow meter monitors the flow of the second solenoid valve; once the flow reaches the set value, the second solenoid valve is closed, and the first solenoid valve is immediately opened, allowing the disinfectant in the drain trough to flow into the collection pipe through the first solenoid valve and finally discharged through the drain port at the bottom of the three-way valve, completing the disinfection and cleaning of the pipeline and effectively reducing cross contamination; after closing the drain port, the disinfectant can flow into the interior of the delivery pipe to achieve disinfection of the delivery pipe; this process ensures the cleanliness and hygiene of the system and provides a safer environment for the collection and treatment of urine samples.

[0033] (3) The automatic urine sample collection and pretreatment system provided by the present invention realizes the automatic collection and pretreatment of urine samples through an integrated sample collection module and a pretreatment system; the system can automatically control the collection, storage, pretreatment, concentration, separation and analysis storage of urine samples, greatly reducing the steps of manual operation, reducing the labor intensity of operators, and improving the speed and efficiency of sample processing; in addition, the automated operation reduces human errors, improves the consistency and repeatability of sample processing, and thus improves the overall experimental and clinical detection quality.

[0034] This solves the problem that the existing system cannot collect urine in sections when collecting urine, which easily leads to deviations in indicators related to the number of bacteria in urine routine. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0036] Figure 2 This is a schematic diagram of the structure of the first solenoid valve and the second solenoid valve of the present invention;

[0037] Figure 3 It is a schematic diagram of the pretreatment system module of the present invention.

[0038] In the figure, 1, pretreatment system; 101, sample collection storage module; 1011, urine sample storage unit; 1012, sample suction unit; 102, sample pretreatment reaction module; 1021, treatment liquid supply unit; 1022, PVDF filter plate supply unit; 1023, protein collection unit; 1024, enzyme storage unit; 1025, lysis reaction container supply unit; 1026, shaking table; 103, sample concentration and separation module; 10 31. Concentrator; 1032. Centrifugal module; 104. Sample analysis storage module; 1041. PCR plate; 1042. Dilution module; 2. Control box; 3. Water inlet; 4. Water tank; 5. Water valve; 6. Urinal; 7. Drain; 8. First solenoid valve; 9. Second solenoid valve; 10. Flow meter; 11. Collection tube; 12. Drain pipe; 13. Three-way valve; 14. Drain outlet; 15. Tray; 16. Drain pipe; 17. Delivery pipe. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Embodiment 1:

[0041] See also Figure 1-3 The embodiment of the present invention provides a technical solution: a urine sample automatic collection and pretreatment system, including a sample automatic collection module and a pretreatment system 1, the sample collection module is used to collect urine samples to ensure the freshness and representativeness of the samples; the pretreatment system 1 includes:

[0042] Sample collection and storage module 101, sample collection and storage module 101 is responsible for collecting and storing urine samples, ensuring that the samples can be safely and hygienically stored after collection until the next step of processing; sample pretreatment reaction module 102, sample pretreatment reaction module 102 involves chemical treatment and biological reaction of urine samples, including adding treatment fluid and separating proteins to prepare samples for further analysis; sample concentration and separation module 103, sample concentration and separation module 103 is responsible for concentrating and separating specific components in urine samples, by reducing sample volume and separating solid particles or cells, so as to facilitate subsequent The sample analysis storage module 104 is used for final sample analysis and result storage, molecular biology analysis, and ensuring the stability of the sample during the analysis and the reliability of the result; the sample collection storage module 101 includes a urine sample storage unit 1011 and a sample suction unit 1012; the urine sample storage unit 1011 is used to store the collected urine sample to ensure that the sample is stored under appropriate conditions to avoid contamination and deterioration; the sample suction unit 1012 includes a discharge gun device, which is used to accurately suck the urine sample from the storage unit to prepare for the next step of processing;

[0043] The sample pretreatment reaction module 102 includes a treatment liquid supply unit 1021, a PVDF filter plate supply unit 1022, a protein collection unit 1023, an enzyme storage unit 1024, a lysis reaction container supply unit 1025 and a shaker 1026; the treatment liquid supply unit 1021 provides lysis liquid, reducing agent, alkylating agent and chemical reagent for pretreatment of urine samples, such as cell lysis and chemical modification; the PVDF filter plate supply unit 1022 provides a PVDF filter plate for adsorbing proteins in urine samples for subsequent separation and analysis; the protein collection unit 1023 collects proteins after filtration or adsorption to prepare for subsequent protein analysis; the enzyme storage unit 1024 stores enzymes for urine sample treatment, such as enzymes for catalyzing specific chemical reactions; the lysis reaction container supply unit 1025 provides a container for holding urine samples and lysis liquid for cell lysis reactions; the shaker 1026 is used to mix urine samples and treatment liquid to ensure that the reaction proceeds evenly; the sample concentration and separation module 103 includes a concentrator 1031 and a centrifugal module Block 1032; concentrator 1031 is used to reduce the volume of urine samples and increase the concentration of target analytes for more accurate analysis; centrifugal module 1032 separates different components in urine samples, such as cells and precipitates, by centrifugal force; sample analysis storage module 104 includes PCR plate 1041 and dilution module 1042; PCR plate 1041 is used to store and process pre-treated urine samples, which is commonly used in molecular biology techniques such as PCR; dilution module 1042 dilutes urine samples according to analysis requirements to adjust the sample concentration to a level suitable for analysis; through the integrated sample collection module and pretreatment system 1, the automatic collection and pretreatment of urine samples are realized; the system can automatically control the collection, storage, pretreatment, concentration, separation and analysis storage of urine samples, greatly reducing the steps of manual operation, reducing the labor intensity of operators, and improving the speed and efficiency of sample processing; in addition, automated operation reduces human errors, improves the consistency and repeatability of sample processing, thereby improving the overall experimental and clinical detection quality.

[0044] Embodiment 2:

[0045] A urine sample automatic collection and pretreatment system, the sample collection module includes a control box 2, the control box 2 is used for the overall control of the system, a urinal 6 is installed on the wall, a drain trough 7 is installed at the bottom of the urinal 6, the drain trough 7 is connected to the hole groove at the bottom of the urinal 6, a first solenoid valve 8 and a second solenoid valve 9 are arranged at one end of the drain trough 7, one end of the first solenoid valve 8 and the second solenoid valve 9 are both connected to the inside of the drain trough 7, the output end of the second solenoid valve 9 is connected to the input end of the flow meter 10, and the output end of the flow meter 10 is connected to the sewer pipe 16 through the drain pipe 12; the first solenoid valve 8 is connected to one end of the collection pipe 11, and the other end of the collection pipe 11 is connected to the three-way valve 13, the three-way valve 13 is provided with a drain port 14 at the bottom, a tray 15 is arranged below the drain port 14, and the surface of the tray 15 is provided with filter holes connected to the downpipe 16. The water pipe 16 is connected, and one side of the three-way valve 13 is connected to the pretreatment system 1 through the delivery pipe 17. When the system collects urine, the urine is discharged into the drain trough 7 through the urinal 6, and then the urine is discharged into the drain pipe 12 through the second solenoid valve 9, and then discharged into the sewer pipe 16 through the drain pipe 12. In this process, the flow of the second solenoid valve 9 is detected by the flow meter 10. When the specified flow value is reached, the second solenoid valve 9 is closed and the first solenoid valve 8 is opened, so that the urine in the drain trough 7 can be discharged into the collection pipe 11 through the first solenoid valve 8, and then delivered to the pretreatment system 1 through the three-way valve 13 and the delivery pipe 17, which is beneficial to avoid the front end of urine collection, thereby helping to reduce the deviation of the number of bacteria in urine and improve the accuracy of the test results.

[0046] Embodiment 3:

[0047] A urine sample automatic collection and pretreatment system, the top of the urinal 6 is connected to the water valve 5, the water valve 5 is connected to the inside of the water tank 4 through a pipeline, the water tank 4 is located above the urinal 6, and the inside of the water tank 4 is filled with disinfectant, and a water inlet 3 is installed on one side of the top of the water tank 4. By opening the water valve, the disinfectant inside the water tank can be discharged into the urinal for cleaning, and then the disinfectant will flow through the drain trough and the second solenoid valve for disinfection and flushing, and by rotating the three-way valve 13, the drain port 14 at the bottom of the three-way valve 13 can be opened, and then by opening the water valve 5, the disinfectant inside the water tank 4 can be discharged into the urinal 6 for cleaning. Cleaning, and then the disinfectant will flow through the drain trough 7 and the second solenoid valve 9 for disinfection and flushing. In this process, the flow of the second solenoid valve 9 is detected by the flow meter 10. When the specified flow value is reached, the second solenoid valve 9 is closed and the first solenoid valve 8 is opened, so that the disinfectant inside the drain trough 7 can be discharged into the collection pipe 11 through the first solenoid valve 8, and then discharged through the drain port 14 at the bottom of the three-way valve 13, so that the pipeline can be disinfected and cleaned, thereby reducing cross contamination, and the disinfectant water can flow into the delivery pipe 17 by closing the drain port 14, thereby disinfecting the inside of the delivery pipe 17.

[0048] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0049] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A urine sample automatic collection and pretreatment system, characterized in that: It comprises an automatic sample collection module and a pretreatment system (1), wherein the sample collection module is used to collect urine samples to ensure the freshness and representativeness of the samples; The pretreatment system (1) comprises: A sample collection and storage module (101), the sample collection and storage module (101) is responsible for collecting and storing urine samples, ensuring that the samples can be stored safely and hygienically after collection until the next step of processing; A sample pre-processing reaction module (102), wherein the sample pre-processing reaction module (102) involves chemical processing and biological reaction of urine samples, including adding processing fluids and separating proteins to prepare the samples for further analysis; A sample concentration and separation module (103), wherein the sample concentration and separation module (103) is responsible for concentrating and separating specific components in the urine sample by reducing the sample volume and separating solid particles or cells to facilitate subsequent analysis and detection; The sample analysis storage module (104) is used for final sample analysis and result storage, for molecular biology analysis, and for ensuring the stability of the sample during the analysis and the reliability of the result.

2. The urine sample automatic collection and pretreatment system according to claim 1, characterized in that: The sample collection and storage module (101) comprises a urine sample storage unit (1011) and a sample suction unit (1012); The urine sample storage unit (1011) is used to store the collected urine samples, ensuring that the samples are stored under appropriate conditions to avoid contamination and deterioration; The sample suction unit (1012) includes a gun discharge device for accurately sucking urine samples from the storage unit in preparation for the next step of processing.

3. The urine sample automatic collection and pretreatment system according to claim 1, characterized in that: The sample pretreatment reaction module (102) comprises a treatment liquid supply unit (1021), a PVDF filter plate supply unit (1022), a protein collection unit (1023), an enzyme storage unit (1024), a lysis reaction container supply unit (1025) and a shaking table (1026); The processing liquid supply unit (1021) provides lysing liquid, reducing agent, alkylating agent and chemical reagent for pre-treatment of urine samples, such as cell lysis and chemical modification; The PVDF filter plate supply unit (1022) provides a PVDF filter plate for adsorbing proteins in urine samples for subsequent separation and analysis; The protein collection unit (1023) collects the filtered or adsorbed proteins to prepare for subsequent protein analysis; The enzyme storage unit (1024) stores enzymes used for urine sample processing, such as enzymes used to catalyze specific chemical reactions; The lysis reaction container supply unit (1025) provides a container for holding a urine sample and a lysis solution to perform a cell lysis reaction; The shaker (1026) is used to mix the urine sample and the treatment solution to ensure that the reaction proceeds evenly.

4. The urine sample automatic collection and pretreatment system according to claim 1, characterized in that: The sample concentration and separation module (103) comprises a concentrator (1031) and a centrifugal module (1032); The concentrator (1031) is used to reduce the volume of the urine sample and increase the concentration of the target analyte for more accurate analysis; The centrifugal module (1032) separates different components in the urine sample, such as cells and sediments, through centrifugal force.

5. The urine sample automatic collection and pretreatment system according to claim 1, characterized in that: The sample analysis storage module (104) comprises a PCR plate (1041) and a dilution module (1042); The PCR plate (1041) is used to store and process pre-treated urine samples and is commonly used in molecular biology techniques such as PCR; The dilution module (1042) dilutes the urine sample according to the analysis requirements to adjust the sample concentration to a level suitable for analysis.

6. The urine sample automatic collection and pretreatment system 1 according to claim 1, characterized in that: The sample concentration and separation module (103) also includes a liquid cooling technology unit for providing efficient thermal management during the concentration and separation of urine samples; the liquid cooling technology unit uses liquid cooling technology to reduce the heat generated during sample processing and maintain the stability and activity of the sample.

7. The urine sample automatic collection and pretreatment system 1 according to claim 1, characterized in that: The sample collection module comprises a control box (2), and the control box (2) is used for overall control of the system. The urinal (6) is mounted on a wall, and a drainage groove (7) is mounted at the bottom of the urinal (6). The drainage groove (7) is connected to a hole groove at the bottom of the urinal (6). A first solenoid valve (8) and a second solenoid valve (9) are arranged at one end of the drainage groove (7). One end of the first solenoid valve (8) and one end of the second solenoid valve (9) are both connected to the inside of the drainage groove (7). The output end of the second solenoid valve (9) is connected to the input end of a flow meter (10), and the output end of the flow meter (10) is connected to a sewer pipe (16) through a drainage pipe (12); The first solenoid valve (8) is connected to one end of the collection pipe (11), the other end of the collection pipe (11) is connected to the three-way valve (13), a drain outlet (14) is provided at the bottom of the three-way valve (13), a tray (15) is provided below the drain outlet (14), a filter hole is provided on the surface of the tray (15) and is connected to a sewer pipe (16), and one side of the three-way valve (13) is connected to the pretreatment system (1) through a delivery pipe (17).

8. The urine sample automatic collection and pretreatment system 1 according to claim 7, characterized in that: The top of the urinal (6) is connected to a water valve (5), and the water valve (5) is connected to the inside of a water tank (4) through a pipeline. The water tank (4) is located above the urinal (6), and the inside of the water tank (4) is filled with disinfectant. A water inlet (3) is installed on one side of the top of the water tank (4).

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