Rodent eyeball injector

Abstract

The invention relates to a rodent eyeball injector. The rodent eyeball injector comprises a main body; the needle insertion depth identification system comprises a needle insertion depth adjusting part and a needle insertion depth visual monitoring part which are arranged on the main body; the injection system comprises a microsyringe detachably connected with the needle insertion depth adjusting part and a hollow sharp needle sleeve detachably connected to the end, close to an injection object, of the main body, and a beveled tip used for beveled cutting to form a pre-cut opening is arranged on the hollow sharp needle sleeve; the microsyringe is connected with a flat-head needle head used for entering the eyeball for injection, and the flat-head needle head can movably penetrate through the hollow sharp needle sleeve. The micro-injector is provided with the beveled tip and the flat-head needle, the needle insertion depth of the micro-injector can be accurately controlled, and a tool which is more efficient, safer, more flexible and easier to operate is provided for eye administration of rodents, so that the quality and efficiency of related research are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of physiological equipment, in particular to a rodent eyeball syringe. Background Art

[0002] In the fields of neuroscience and ophthalmology, topical drug delivery or administration to the eyes of rodents (such as mice and rats) is a key technology. These experiments are crucial for understanding the workings of the visual system, testing new treatments, and evaluating drug effects. However, existing microinjector designs have several limitations, primarily due to the uniformity of the needle shape and the lack of control over the insertion depth.

[0003] The problems existing in the prior art are mainly:

[0004] (1) Needle shape problem - needle shape and function are single:

[0005] Most of the ocular microinjectors currently on the market are equipped with sharp needles, which is conducive to penetrating the cornea, but is not suitable for all types of experiments. Most existing microinjectors are only equipped with pointed needles with a bevel angle of 12° or flat-tipped needles. Pointed needles are very effective when it is necessary to penetrate the cornea. However, in some experiments, such as when it is necessary to avoid damage to sensitive structures in the eye or when it is desired to distribute the drug more evenly, flat-tipped needles are more suitable. The single needle design limits the flexibility and adaptability of experimental operations.

[0006] For example, researchers may need to use a flat-tip needle to avoid damaging sensitive structures in the eye, or to distribute drugs more evenly. Therefore, in the recently improved experimental operation, a sharp needle is first used to form a pre-incision in the eyeball, the needle is withdrawn, and then a flat-tip needle is replaced to enter the pre-incision in the eyeball for a second time to inject the sample. Although this method avoids the intraocular damage caused by the sharp needle, the operation of changing the needle twice is complicated. The pre-incision in the eyeball formed by the sharp needle for the first time has no obvious marking site, and the cumbersome operation of changing the needle requires the operator to take his eyes off the pre-incision in the eyeball, resulting in forgetting the original position of the pre-incision, which increases the difficulty of secondary injection.

[0007] (2) Insufficient control of needle insertion depth - lack of needle insertion depth marking: The micro-injectors on the market do not provide clear needle insertion depth indications. Due to the lack of significant and effective needle insertion depth markings, it is difficult for the operator to accurately judge and control the depth and position of the needle into the eye. The uncertainty of blind operation not only increases the difficulty of the experiment, but may also cause unnecessary intraocular damage (tissue damage) or inaccurate drug dosage, thereby affecting the validity and reliability of the experimental results.

[0008] (3) High operational complexity:

[0009] Even though some advanced micro-injectors are equipped with blind stop sleeves to assist in controlling the depth of the needle, these devices usually require estimating the needle depth in advance and using a ruler to adjust the position of the blind stop. During operation, it is often easy to accidentally touch the blind stop, causing the position to change, and the adjustment process is not intuitive enough.

[0010] Therefore, the inventors, relying on their many years of experience and practice in related industries, have proposed a rodent eyeball syringe to overcome the defects of the prior art. Summary of the Invention

[0011] The purpose of the present invention is to provide a rodent eye syringe to solve the technical problems that existing rodent eye micro-syringes lack both penetration and injection functions and lack a needle insertion depth indicator. The present invention has both a beveled tip and a flat-head needle, and can accurately control the needle insertion depth of the micro-syringe, providing a more efficient, safe, flexible and easy-to-operate tool for rodent eye drug delivery, thereby improving the quality and efficiency of related research.

[0012] The object of the present invention is achieved by a rodent eyeball syringe comprising:

[0013] main body;

[0014] A needle insertion depth marking system, comprising a needle insertion depth adjustment portion and a needle insertion depth visual monitoring portion provided on the main body;

[0015] The injection system comprises a microinjector detachably connected to the needle insertion depth adjustment portion and a hollow sharp needle sleeve detachably connected to an end of the main body proximal to the injection target, the hollow sharp needle sleeve being provided with a beveled tip for beveling to form a pre-cut; a flat-headed needle for injecting into the interior of the eyeball is connected to the microinjector, the flat-headed needle being movably inserted through the hollow sharp needle sleeve.

[0016] In a preferred embodiment of the present invention, the needle insertion depth adjustment part includes a movable control knob, a screw, and a sliding nut. The movable control knob is rotatably connected to the main body and is at least partially exposed from the main body. The movable control knob is used to manually adjust the position of the micro-injection needle to adjust the needle insertion depth; the screw is rotatably arranged in the main body, and the axial direction of the screw is parallel to the axial direction of the injection system. The first end of the screw is passed through the movable control knob, and the screw can rotate synchronously with the movable control knob, and the second end of the screw is hinged to the main body; the sliding nut is circumferentially fixedly sleeved on the screw, and the micro-injection syringe can be detachably connected to the sliding nut to move with the sliding nut.

[0017] In a preferred embodiment of the present invention, the sliding nut is connected to a metal fixing plate located on the outside of the main body, and at least one magnetic fixing buckle is magnetically connected to the metal fixing plate, and the position of the magnetic fixing buckle on the metal fixing plate is adjustably set; a syringe card interface is set on the magnetic fixing buckle, and the micro syringe is clamped in the syringe card interface.

[0018] In a preferred embodiment of the present invention, the needle insertion depth visualization monitoring unit includes a scale display unit provided on the main body, and the scale display unit is provided on one side of the metal fixing plate.

[0019] In a preferred embodiment of the present invention, the main body is connected to a transparent outer cover that can be rotated to open or snapped closed.

[0020] In a preferred embodiment of the present invention, the hollow sharp needle guard is connected to a needle guard holder, and one end of the needle guard holder is provided with a connecting unit that can be detachably connected to the main body.

[0021] In a preferred embodiment of the present invention, the needle sleeve holder includes a semi-tapered sleeve, the diameter of the semi-tapered sleeve at one end close to the main body is larger than the diameter of the end away from the main body, a connecting post is provided at the end of the semi-tapered sleeve close to the main body, a connecting thread is provided on the connecting post, and the connecting post and the connecting thread constitute the connecting unit; the length of the semi-tapered sleeve is smaller than the length of the hollow sharp needle sleeve, and the side wall of the hollow sharp needle sleeve is connected to the semi-tapered sleeve at a position close to the main body.

[0022] In a preferred embodiment of the present invention, the hollow sharp needle sleeve is arranged in a conical sleeve structure, and the diameter of the end of the hollow sharp needle sleeve close to the main body is larger than the diameter of the end away from the main body; the tip angle of the beveled tip of the hollow sharp needle sleeve is in the range of 12° to 30°.

[0023] In a preferred embodiment of the present invention, the hollow sharp needle sleeve is made of stainless steel.

[0024] In a preferred embodiment of the present invention, the main body, the needle insertion depth marking system and the injection system are all made of high temperature and high pressure sterilization resistant materials.

[0025] As described above, the rodent eyeball syringe of the present invention has the following beneficial effects:

[0026] The present invention can support a variety of needle types: the hollow sharp needle sleeve with a beveled tip can realize the function of a pointed needle. Combined with the flat-tip needle connected to the micro-injector, the present invention is equivalent to having both a pointed needle and a flat-tip needle. The beveled tip is used to form a pre-incision, pushing the flat-tip needle into the eyeball for injection, reducing the number of needle insertions, avoiding the tedious operation of multiple needle changes, and reducing damage to intraocular tissues caused by the operation.

[0027] The microinjector and hollow sharp needle sleeve are both detachably connected to the main body, allowing for flexible switching and quick replacement of the hollow sharp needle sleeve and blunt-tip needle, adapting to different eye surgery needs and supporting a variety of needle types. This invention improves the flexibility of the syringe, enabling it to adapt to a wider range of operations, thereby expanding its application range.

[0028] This device precisely controls needle insertion depth. The depth adjustment mechanism ensures that each injection reaches the preset depth, enhancing the safety and accuracy of eyeball injection procedures. The visual depth monitoring system provides an intuitive reference for real-time needle depth monitoring, preventing eye damage caused by over-insertion. The device boasts high repeatability and a stable mechanical structure, ensuring consistent settings from time to time, contributing to reliable experimental results or therapeutic outcomes.

[0029] The present invention enhances the ease of operation and is user-friendly in design: the concise and intuitive operating interface enables even novices to quickly master the usage method, reducing training time and cost; the overall design of the device is compact and light, making it easy to carry and store, and suitable for use in different laboratories or surgical environments.

[0030] The present invention can improve surgical efficiency and success rate and reduce surgical time: since the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the surgical process is accelerated; precise control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, improves the success rate of surgery and the safety of experimental animals.

[0031] This device ensures sterility and is easy to disinfect: The design of each device component takes into account the requirements of aseptic operation. All parts that come into contact with the drug can be easily disassembled, cleaned, and sterilized at high temperature and high pressure, ensuring the sterility of the surgical environment. Disposable component options: To further enhance safety, disposable needle covers and other key components can be used to prevent cross-infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The following drawings are only intended to illustrate and explain the present invention, and are not intended to limit the scope of the present invention.

[0033] in:

[0034] Figure 1It is a front view of the rodent eyeball syringe of the present invention.

[0035] Figure 2 1 is a side view of the rodent eyeball syringe of the present invention.

[0036] Figure 3 This is a front view of the internal structure of the rodent eyeball syringe of the present invention.

[0037] Figure 4 It is a side view of the internal structure of the rodent eyeball syringe of the present invention.

[0038] In the picture:

[0039] 1. Subject;

[0040] 2. Move the control knob;

[0041] 3. Magnetic fixing buckle;

[0042] 4. Metal fixing plate;

[0043] 5. Scale display part;

[0044] 6. Outer cover;

[0045] 7. Hollow sharp needle sleeve;

[0046] 8. Needle sleeve holder;

[0047] 9. Screw;

[0048] 10. Sliding nut;

[0049] 11. Connection unit. DETAILED DESCRIPTION

[0050] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0051] The specific embodiments of the present invention described herein are intended only to illustrate the present invention and are not to be construed as limiting the present invention in any way. In light of the present invention, a skilled person may conceive of any possible variations based on the present invention, all of which should be considered to fall within the scope of the present invention. It should be noted that when an element is referred to as being "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected" to another element, it may be directly connected to the other element or there may be an intermediate element at the same time. The terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to mechanical or electrical connections, or to internal communication between two elements, and may be directly connected or indirectly connected through an intermediate medium. A person of ordinary skill in the art can understand the specific meanings of the above terms based on the specific circumstances. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are intended only to describe specific embodiments and are not intended to limit this application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0053] The present invention aims to provide a more efficient, safe, flexible and easy-to-operate tool for ocular drug delivery in rodents, thereby improving the quality and efficiency of related research.

[0054] like Figures 1 to 4 As shown, the present invention provides a rodent eyeball syringe, comprising:

[0055] Main body 1: Main body 1 serves as the basic framework of the entire device, supporting and connecting all other components.

[0056] The needle insertion depth marking system includes a needle insertion depth adjustment portion and a needle insertion depth visualization monitoring portion provided on the main body 1. The needle insertion depth marking system can accurately control the needle insertion depth of the micro-injector.

[0057] The injection system includes a micro-injector detachably connected to a needle insertion depth adjustment portion, and a micro-injector in the prior art, such as a Hamilton micro-injector (33G, flat-tipped needle) can be used; the injection system also includes a hollow sharp needle sleeve 7 detachably connected to the end of the main body 1 close to the injection object, and the hollow sharp needle sleeve 7 is provided with a beveled tip for beveling to form a pre-cut; the micro-injector is connected to a flat-tipped needle for injecting into the interior of the eyeball, and the flat-tipped needle can be movably inserted through the hollow sharp needle sleeve 7.

[0058] The present invention can support a variety of needle types: the hollow sharp needle sleeve 7 with a beveled tip can realize the function of a pointed needle. Combined with the flat-tip needle connected to the micro-injector, the present invention is equivalent to having both a pointed needle and a flat-tip needle. The beveled tip is used to form a pre-incision, pushing the flat-tip needle into the eyeball for injection, reducing the number of needle insertions, avoiding the tedious operation of multiple needle changes, and reducing damage to intraocular tissues caused by the operation.

[0059] The microinjector and hollow sharp needle sheath 7 are both detachably connected to the main body 1, allowing for flexible switching and quick replacement of the hollow sharp needle sheath 7 with a flat-tip needle, adapting to different eye surgery requirements and supporting a variety of needle types. This invention enhances the flexibility of the syringe, enabling it to adapt to a wider range of operations, thereby expanding its application range.

[0060] This device precisely controls needle insertion depth. The depth adjustment mechanism ensures that each injection reaches the preset depth, enhancing the safety and accuracy of eyeball injection procedures. The visual depth monitoring system provides an intuitive reference for real-time needle depth monitoring, preventing eye damage caused by over-insertion. The device boasts high repeatability and a stable mechanical structure, ensuring consistent settings from time to time, contributing to reliable experimental results or therapeutic outcomes.

[0061] The present invention enhances the ease of operation and is user-friendly in design: the concise and intuitive operating interface enables even novices to quickly master the usage method, reducing training time and cost; the overall design of the device is compact and light, making it easy to carry and store, and suitable for use in different laboratories or surgical environments.

[0062] The present invention can improve surgical efficiency and success rate and reduce surgical time: since the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the surgical process is accelerated; precise control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, improves the success rate of surgery and the safety of experimental animals.

[0063] This device ensures sterility and is easy to disinfect: The design of each device component takes into account the requirements of aseptic operation. All parts that come into contact with the drug can be easily disassembled, cleaned, and sterilized at high temperature and high pressure, ensuring the sterility of the surgical environment. Disposable component options: To further enhance safety, disposable needle covers and other key components can be used to prevent cross-infection.

[0064] To verify the effectiveness and reliability of this invention, multiple animal experiments were conducted. The results showed that during eye injection surgeries using this device, the intended needle insertion depth was achieved in all specimens, and no eye damage due to improper operation occurred. Compared with traditional injection devices, the device of this invention reduced surgical time by an average of approximately 30% and increased the success rate by approximately 20%. This experimental evidence not only demonstrates the technical superiority of this invention but also provides a solid foundation for its widespread application.

[0065] Further, if Figure 3 、 Figure 4 As shown, the needle insertion depth adjustment part includes a movable control knob 2, a screw 9, and a sliding nut 10. The movable control knob 2 is rotatably connected to the main body 1 and is at least partially exposed from the main body 1. The movable control knob 2 is used to manually adjust the position of the micro-injection needle to adjust the needle insertion depth; the screw 9 is rotatably arranged in the main body 1, and the axial direction of the screw 9 is parallel to the axial direction of the injection system. The first end of the screw 9 is passed through the movable control knob 2, and the screw 9 can rotate synchronously with the movable control knob 2. The second end of the screw 9 is hinged to the main body 1; the sliding nut 10 is circumferentially fixedly sleeved on the screw 9, and the micro-injection syringe can be detachably connected to the sliding nut 10 to move with the sliding nut 10.

[0066] The needle insertion depth adjustment part has a fine-tuning function, which can achieve millimeter-level adjustment of the needle position by moving the control knob 2 to ensure that each injection can reach the preset depth.

[0067] In a specific embodiment, the screw 9 has a thread specification of M8, an outer diameter of 8 mm, a pitch of 1.25 mm, and a length of 100 mm; the sliding nut 10 has a threaded hole diameter of 7.06 mm, a width across flats of 13 mm, and a thickness of 6 mm.

[0068] like Figure 3 、 Figure 4 As shown, the top of the screw 9 is connected to the movement control knob 2, providing a stable reference point. By rotating the movement control knob 2, the sliding nut 10 can move along its axial direction ( Figure 3 vertical direction), thereby driving the microinjector to move forward or backward steadily.

[0069] Further, if Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 As shown, the sliding nut 10 is connected to a metal fixing plate 4 (the metal here refers to ferromagnetic metal) located on the outside of the main body, and at least one magnetic fixing buckle 3 is magnetically connected to the metal fixing plate 4. It is generally better to set the magnetic fixing buckles 3 in pairs. The position of the magnetic fixing buckle 3 on the metal fixing plate 4 is adjustable; a syringe card interface is provided on the magnetic fixing buckle 3, and the micro-syringe is connected to the syringe card interface. Specifically, the syringe card interface is connected to the syringe barrel of the micro-syringe, one end of the injection piston is sealed and slidably inserted into the syringe barrel, and the other end of the injection piston passes through the main body 1 and is exposed, which is convenient for adjusting the injection depth after the needle is inserted.

[0070] The back of the magnetic fastener 3 has a magnetic patch that attaches to the metal mounting plate 4. The inner diameter of the syringe interface of the magnetic fastener 3 is 6mm, which can accommodate a glass micro-syringe with an outer diameter of 6.6mm, ensuring that the micro-syringe is securely mounted at the front end of the device. (The syringe interface of the magnetic fastener 3 is made of silicone material, which has a certain degree of plasticity. Therefore, the inner diameter of the syringe interface is slightly smaller than the outer diameter of the syringe to ensure reliable fixation.) In one specific embodiment, the length of the magnetic fastener 3 along the metal mounting plate 4 is 7mm.

[0071] One side of the metal fixing plate 4 is fixedly connected to the sliding nut 10 , and the other side is connected to the micro syringe, forming a stabilizing system for fixing the micro syringe.

[0072] By rotating the control knob 2, the sliding nut 10 can move along its axial direction ( Figure 3 The microinjector is moved forward or backward steadily by changing the position of the metal fixing plate 4. This design allows for precise control of the needle insertion depth while maintaining ease of operation.

[0073] The present invention has high repetitive accuracy and a stable mechanical structure, which ensures consistency of each setting and helps to obtain reliable experimental results or treatment effects.

[0074] Further, if Figure 1 As shown, the visual needle depth monitoring unit includes a graduated display 5 mounted on the main body 1. Scale display 5 is located on one side of the metal fixing plate 4, facilitating observation and setting of the specific needle insertion depth. In one embodiment, the units of scale display 5 are millimeters (mm). This unit enables visual monitoring of needle insertion, providing the user with an intuitive reference for real-time needle depth monitoring and preventing eye damage caused by over-insertion.

[0075] The present invention introduces a complete needle insertion depth marking system, including a graduated display unit 5 and a needle insertion depth adjustment unit. The needle insertion depth adjustment unit, based on a screw-nut mechanism, precisely advances the microinjection needle (flat-tip needle) by moving the control knob 2. Combined with the graduated display unit 5, this helps researchers more accurately control the depth of needle insertion into the eye, reducing the risk of misoperation and improving experimental consistency and safety.

[0076] Further, if Figure 1 、 Figure 2 As shown, the main body 1 is connected to a transparent outer cover 6 that can be rotated open or snapped closed. The outer cover 6 protects the internal mechanical structure while allowing the user to view the scale display 5 (scale lines) to accurately set the needle insertion depth, providing intuitive visual feedback to ensure operational accuracy.

[0077] Further, if Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 As shown, the hollow, sharp needle guard 7 is connected to the needle guard holder 8. The hollow, sharp needle guard 7 and the needle guard holder 8 form a single, removable and replaceable unit. One end of the needle guard holder 8 is provided with a connecting unit 11 that is removably connected to the main body 1 (note that the connecting unit 11 is not connected to the screw 9; there is a space between the two). The needle guard holder 8 ensures that the hollow, sharp needle guard 7 is securely mounted, preventing it from loosening during use.

[0078] Further, if Figure 4 As shown, the needle sleeve holder 8 includes a semi-tapered sleeve, the diameter of the semi-tapered sleeve at one end close to the main body 1 is larger than the diameter of the end away from the main body 1, a connecting column is provided at the end of the semi-tapered sleeve close to the main body 1, and a connecting thread is provided on the connecting column, and the connecting column and the connecting thread constitute a connecting unit; the length of the semi-tapered sleeve is smaller than the length of the hollow sharp needle sleeve 7, and the side wall of the hollow sharp needle sleeve 7 is connected to the semi-tapered sleeve at a position close to the main body 1.

[0079] In a specific embodiment, the diameter of the semi-conical sleeve at one end close to the main body 1 is 7 mm, the diameter of the end away from the main body 1 is 5.5 mm, and the axial length is 30 mm.

[0080] Furthermore, the hollow sharp needle sleeve 7 is arranged in a conical sleeve structure, and the diameter of the end of the hollow sharp needle sleeve 7 close to the main body 1 is larger than the diameter of the end away from the main body 1; the tip angle of the beveled tip of the hollow sharp needle sleeve 7 ranges from 12° to 30°, and the best is 12° or 30°.

[0081] In one embodiment, the hollow sharp needle sheath 7 is made of stainless steel. The total length of the hollow sharp needle sheath 7 can be: 30mm, 40mm, 50mm, 60mm, or 70mm; the inner and outer diameters of the hollow sharp needle sheath 7 can be: ① 0.25mm-0.35mm, ② 0.4mm-0.65mm; and the tip angle can be: 12° or 30°. These can be selected and used according to actual needs.

[0082] The total length, inner diameter-outer diameter and tip angle of the stainless steel hollow sharp needle sheath 7 can be selected according to actual needs to adapt to different surgical requirements; and it can be used in conjunction with a micro-injector to quickly complete pre-incision and injection operations.

[0083] Furthermore, all components of the present invention, namely the main body 1, the needle insertion depth marking system, and the injection system, are made of high-temperature and high-pressure sterilization-resistant materials, allowing for thorough cleaning and disinfection before and after repeated use. This ensures both hygienic safety and reduced long-term cost.

[0084] Example

[0085] 1. Experimental Materials and Equipment

[0086] Rodent eye syringe (micro-injection device): As described above, it includes a main body 1, a movement control knob 2, a magnetic fixing buckle 3, a metal fixing plate 4, a scale display part 5, an outer cover 6, a hollow sharp needle sleeve 7, a needle sleeve holder 8, a screw 9, a sliding nut 10, a connecting unit 11, and a Hamilton micro-syringe (33G, flat-head needle).

[0087] Experimental animals: healthy adult mice (the specific number is determined according to the experimental design), which have adapted to the laboratory environment and have been properly anesthetized.

[0088] Drugs: ophthalmic drugs that require microinjection, such as isoflurane and tropicamide, or normal saline as a control.

[0089] Other tools: inhalation anesthesia machine, sterile operating table, microscope, disinfection supplies, etc.

[0090] 2. Experimental steps

[0091] 1. Preparation

[0092] Select the needle type: Choose the appropriate needle sleeve according to the specific experimental requirements (for example, a beveled tip for subretinal injections, a flat-tipped needle for intravitreal injections). Mount the Hamilton microsyringe to the front end of the device using the needle sleeve holder 8 and the magnetic fixing buckle 3, ensuring a secure and tight fit. Carefully insert a 30mm long, 0.25mm-0.35mm inner and outer diameter hollow sharp needle sleeve 7 with a 12° bevel angle through the microsyringe needle, preventing the inner wall of the hollow sharp needle sleeve 7 from contacting the syringe needle (flat-tipped needle). Secure the hollow sharp needle sleeve 7 to the main body 1 via the connecting unit.

[0093] Calibrate the needle depth: Rotate the control knob 2 to move the sliding nut 10 along the screw 9, adjusting the position of the metal fixing plate 4 until the microsyringe needle extends to the desired depth. The scale display 5, visible through the transparent cover, allows intuitive monitoring and adjustment of the needle depth, ensuring each injection achieves the desired effect.

[0094] Sterilization: All parts that come into contact with drugs must be rigorously cleaned and sterilized at high temperatures and high pressures to ensure a sterile surgical environment. At the same time, mice must be properly anesthetized to ensure they remain still throughout the procedure.

[0095] 2. Implementation of injection

[0096] Positioning and fixation: Find the target injection site of the eyeball under a microscope and gently fix the mouse head with appropriate clamps to ensure that its eyes are in the correct position.

[0097] Needle Insertion: Hold the rodent eye syringe and slowly and steadily insert the hollow, sharp needle sheath 7 into the target area to create a pre-incision. Rotate the control knob 2 to slowly push the flat-tipped needle into the eyeball and inject to the pre-set depth. During this procedure, the operator should maintain a steady hand to avoid accidental injury due to shaking or other external interference.

[0098] Drug injection: Once the needle reaches the desired depth, gently push the piston of the microsyringe to accurately inject the required dose of medication into the eye. The injection speed should be moderate to avoid causing a sudden increase in intraocular pressure due to excessive injection.

[0099] Needle Retraction: After the injection is complete, slowly withdraw the flat-tipped needle by moving the control knob 2 to avoid bleeding or tissue damage caused by rapid withdrawal. When withdrawing the needle, be sure to maintain the same direction of the needle to minimize impact on surrounding tissue.

[0100] 3. Postoperative Treatment

[0101] Observation and Recording: Immediately after the injection, observe the mouse's eye for bleeding, swelling, or other abnormalities. Detailed records of each injection, such as needle insertion depth, injection volume, and injection site, should be kept for subsequent analysis.

[0102] Recovery Care: Place the mice in a warm, quiet environment and monitor their recovery until they are fully awake. If any mice show any discomfort, take appropriate treatment measures promptly.

[0103] Cleaning and Maintenance: After each experiment, clean the microinjection device promptly, especially the part that comes into direct contact with the drug, to ensure that no residue will affect the next use. Regularly inspect and maintain the device to ensure it is always in optimal working condition.

[0104] 3. Experimental Results and Discussion

[0105] The effectiveness and reliability of the rodent eyeball syringe of the present invention were verified by microinjection experiments on multiple mice:

[0106] Safety: All samples achieved the intended needle insertion depth, and no eye damage due to improper operation occurred. This demonstrates that the device can effectively protect ocular structures and reduce surgical risks.

[0107] Accuracy: Compared with traditional injection devices, the surgical time using this invention is shortened by an average of about 30%, and the surgical success rate is increased by about 20%. This is due to its precise needle insertion depth control mechanism and stable mechanical structure.

[0108] Applicability: Since it supports multiple types of needles and can flexibly adjust the needle insertion depth, the device is suitable for different ophthalmic experimental needs and has a wide range of applications.

[0109] IV. Conclusion

[0110] This example demonstrates the superior performance of the rodent eye syringe of the present invention in a mouse eye microinjection drug administration experiment. It not only improves the safety and accuracy of the surgery, but also simplifies the operation process, providing strong technical support for animal ophthalmic surgery research.

[0111] As described above, the rodent eyeball syringe of the present invention has the following beneficial effects:

[0112] The present invention can support a variety of needle types: the hollow sharp needle sleeve with a beveled tip can realize the function of a pointed needle. Combined with the flat-tip needle connected to the micro-injector, the present invention is equivalent to having both a pointed needle and a flat-tip needle. The beveled tip is used to form a pre-incision, pushing the flat-tip needle into the eyeball for injection, reducing the number of needle insertions, avoiding the tedious operation of multiple needle changes, and reducing damage to intraocular tissues caused by the operation.

[0113] The microinjector and hollow sharp needle sheath 7 are both detachably connected to the main body, allowing for flexible switching and quick replacement of the hollow sharp needle sheath and the blunt needle, adapting to different eye surgery requirements and supporting a variety of needle types. The present invention improves the flexibility of the syringe, enabling it to adapt to a wider range of operations, thereby expanding its application range.

[0114] This device precisely controls needle insertion depth. The depth adjustment mechanism ensures that each injection reaches the preset depth, enhancing the safety and accuracy of eyeball injection procedures. The visual depth monitoring system provides an intuitive reference for real-time needle depth monitoring, preventing eye damage caused by over-insertion. The device boasts high repeatability and a stable mechanical structure, ensuring consistent settings from time to time, contributing to reliable experimental results or therapeutic outcomes.

[0115] The present invention enhances the ease of operation and is user-friendly in design: the concise and intuitive operating interface enables even novices to quickly master the usage method, reducing training time and cost; the overall design of the device is compact and light, making it easy to carry and store, and suitable for use in different laboratories or surgical environments.

[0116] The present invention can improve surgical efficiency and success rate and reduce surgical time: since the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the surgical process is accelerated; precise control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, improves the success rate of surgery and the safety of experimental animals.

[0117] This device ensures sterility and is easy to disinfect: The design of each device component takes into account the requirements of aseptic operation. All parts that come into contact with the drug can be easily disassembled, cleaned, and sterilized at high temperature and high pressure, ensuring the sterility of the surgical environment. Disposable component options: To further enhance safety, disposable needle covers and other key components can be used to prevent cross-infection.

[0118] To verify the effectiveness and reliability of this invention, multiple animal experiments were conducted. The results showed that during eye injection surgeries using this device, the intended needle insertion depth was achieved in all specimens, and no eye damage due to improper operation occurred. Compared with traditional injection devices, the device of this invention reduced surgical time by an average of approximately 30% and increased the success rate by approximately 20%. This experimental evidence not only demonstrates the technical superiority of this invention but also provides a solid foundation for its widespread application.

[0119] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by those skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A rodent eyeball syringe, characterized in that: include: main body; A needle insertion depth marking system, comprising a needle insertion depth adjustment unit and a needle insertion depth visualization monitoring unit provided on the main body; The injection system comprises a micro-injection syringe detachably connected to the needle insertion depth adjustment part and a hollow sharp needle sleeve detachably connected to an end of the main body close to the injection object, wherein the hollow sharp needle sleeve is provided with an oblique cutting tip for oblique cutting to form a pre-cut; the micro-injection syringe is connected to a flat-head needle for entering the inside of the eyeball for injection, and the flat-head needle is movably arranged to pass through the hollow sharp needle sleeve.

2. The rodent eyeball syringe according to claim 1, characterized in that: The needle insertion depth adjustment part includes a movable control knob, a screw, and a sliding nut. The movable control knob is rotatably connected to the main body and at least partially exposed from the main body. The movable control knob is used to manually adjust the position of the micro-injection needle to adjust the needle insertion depth; the screw is rotatably arranged in the main body, and the axial direction of the screw is parallel to the axial direction of the injection system. The first end of the screw is inserted into the movable control knob, and the screw can rotate synchronously with the movable control knob, and the second end of the screw is hinged to the main body; the sliding nut is circumferentially fixedly sleeved on the screw, and the micro-injection syringe can be detachably connected to the sliding nut to move with the sliding nut.

3. The rodent eyeball syringe according to claim 2, characterized in that: The sliding nut is connected to a metal fixing plate located on the outside of the main body, and the metal fixing plate is magnetically connected to at least one magnetic fixing buckle, and the position of the magnetic fixing buckle on the metal fixing plate is adjustably set; a syringe card interface is set on the magnetic fixing buckle, and the micro-syringe is clamped in the syringe card interface.

4. The rodent eyeball syringe according to claim 3, characterized in that: The needle insertion depth visualization monitoring unit includes a scale display unit arranged on the main body, and the scale display unit is arranged on one side of the metal fixing plate.

5. The rodent eyeball syringe according to claim 1, characterized in that: The main body is connected with a transparent outer cover which can be rotated to open or snapped to close.

6. The rodent eyeball syringe according to claim 1, characterized in that: The hollow sharp needle sleeve is connected to the needle sleeve holder, and one end of the needle sleeve holder is provided with a connecting unit which is detachably connected to the main body.

7. The rodent eyeball syringe according to claim 6, characterized in that: The needle sleeve holder comprises a semi-taper sleeve, wherein the diameter of one end of the semi-taper sleeve close to the main body is larger than the diameter of the other end away from the main body, a connecting column is arranged at one end of the semi-taper sleeve close to the main body, a connecting thread is arranged on the connecting column, and the connecting column and the connecting thread constitute the connecting unit; the length of the semi-taper sleeve is smaller than the length of the hollow sharp needle sleeve, and the side wall of the hollow sharp needle sleeve is connected to the semi-taper sleeve at a position close to the main body.

8. The rodent eyeball syringe according to claim 1, characterized in that: The hollow sharp needle sleeve is arranged in a cone sleeve structure, and the diameter of the end of the hollow sharp needle sleeve close to the main body is larger than the diameter of the end away from the main body; the tip angle of the beveled tip of the hollow sharp needle sleeve ranges from 12° to 30°.

9. The rodent eyeball syringe according to claim 1, characterized in that: The hollow sharp needle sleeve is made of stainless steel.

10. The rodent eyeball syringe according to claim 1, characterized in that: The main body, the needle insertion depth marking system and the injection system are all made of high temperature and high pressure sterilization resistant materials.

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