A dental caries-preventing oral care composition containing patchouli essential oil and application thereof

Abstract

The present invention discloses an anti-caries oral care composition containing patchouli essential oil and its application. The composition also includes at least one of cinnamon essential oil or amomum essential oil. The present invention creatively combines patchouli essential oil with cinnamon essential oil or amomum essential oil (or a mixture of the two), and gives full play to the synergistic effect between the essential oil components through a scientific compounding strategy. This compounding scheme is intended to act efficiently on oral cells to achieve the inhibition of harmful microorganisms. Specifically, the composition not only has significant antibacterial and anti-inflammatory functions, can effectively reduce the number of pathogenic bacteria in the oral cavity, but also can inhibit the formation of dental plaque and slow down the development of caries through a continuous mechanism of action. In addition, the invention also demonstrates comprehensive care for oral health, providing multiple protective effects including improving breath and enhancing gum health, thereby comprehensively improving the overall defense capability and comfort of the oral cavity.

Description

Technical Field

[0001] The invention relates to the technical field of oral care, and in particular to an anti-caries oral care composition containing patchouli essential oil and application thereof. Background Art

[0002] Patchouli essential oil exhibits rich pharmacological activities and excellent antibacterial properties. 130 chemical substances have been identified in its composition, among which the sesquiterpenoid patchouli alcohol is the most critical active ingredient. These ingredients together give patchouli essential oil significant antibacterial and anti-inflammatory effects. Modern scientific research has further verified the antibacterial activity of patchouli essential oil, which can effectively curb the growth of oral bacteria and plays a key role in preventing oral diseases such as caries. Therefore, it has attracted increasing attention in the application of oral care products.

[0003] As the public pays more attention to oral health, oral care products tend to use natural ingredients. Patchouli essential oil, as a natural plant essential oil, not only has excellent antibacterial ability, but also can optimize the oral microenvironment and improve the overall oral health level. Therefore, its application in oral care products such as toothpaste has become an emerging trend.

[0004] Although there are many anti-caries toothpastes on the market, some products rely on chemical ingredients such as fluoride, which may pose potential risks to human health if used for a long time. In contrast, patchouli essential oil, as a natural ingredient, has been widely verified for its safety and effectiveness, making it more suitable for the development of anti-caries toothpaste.

[0005] As consumers' health awareness increases, they have higher requirements for the naturalness and environmental friendliness of products. Patchouli essential oil, as a natural plant essential oil, conforms to this consumption trend and can better meet market demand. Therefore, the development of anti-caries toothpaste containing patchouli essential oil not only meets the current consumer's pursuit of health, naturalness and environmental protection, but also fills the gap in the market for toothpaste that effectively inhibits dental plaque and caries. Summary of the invention

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides an anti-caries oral care composition containing patchouli essential oil, which can inhibit the formation of dental plaque and caries for a long time while having antibacterial and anti-inflammatory effects.

[0007] The present invention also provides the use of the anticaries oral care composition.

[0008] According to the first aspect of the present invention, an anti-caries oral care composition containing patchouli essential oil further comprises at least one of cinnamon essential oil or amomum villosum essential oil.

[0009] According to the anti-caries oral care composition containing patchouli essential oil according to the embodiment of the present invention, there are at least the following beneficial effects: the present invention creatively combines patchouli essential oil with cinnamon essential oil or Amomum villosum essential oil (or a mixture of the two), and gives full play to the synergistic effect between the essential oil components through a scientific compounding strategy. This compounding scheme is intended to act efficiently on oral cells to achieve the inhibition of harmful microorganisms. Specifically, the composition not only has significant antibacterial and anti-inflammatory functions, can effectively reduce the number of pathogenic bacteria in the oral cavity, but also through a continuous mechanism of action, can long-term inhibit the formation of dental plaque and slow down the development of caries. In addition, the invention also demonstrates comprehensive care for oral health, providing multiple protective effects including improving breath and enhancing gum health, thereby comprehensively improving the overall defense capability and comfort of the oral cavity.

[0010] According to some embodiments of the present invention, the composition further comprises at least one of Litsea cubeba essential oil, Kaempferia galanga essential oil or the seasoning Osmanthus fragrans essential oil.

[0011] According to some embodiments of the present invention, the patchouli essential oil is derived from essential oil preparation methods such as steam distillation, supercritical carbon dioxide extraction, or is purchased commercially.

[0012] According to some embodiments of the present invention, the cinnamon essential oil, litsea cubeba essential oil, galangal essential oil, amomum villosum essential oil, and the seasoning osmanthus fragrans essential oil are derived from essential oil preparation methods such as steam distillation and supercritical carbon dioxide extraction or are purchased commercially.

[0013] According to some embodiments of the present invention, the weight percentage of patchouli essential oil in the composition is 6 to 99.9%.

[0014] According to some embodiments of the present invention, the composition contains patchouli essential oil, cinnamon essential oil, litsea cubeba essential oil, kaempferia galanga essential oil, amomum villosum essential oil and seasoning osmanthus fragrans essential oil, and the mass ratio of patchouli essential oil, cinnamon essential oil, litsea cubeba essential oil, kaempferia galanga essential oil, amomum villosum essential oil and seasoning osmanthus fragrans essential oil is 1:16-20:8-20:16-20:256-320:128-160.

[0015] According to some embodiments of the present invention, the composition includes the following raw materials in parts by weight: 0.0025-0.04 parts of patchouli essential oil, 0.04-0.8 parts of cinnamon essential oil, 0.02-0.4 parts of litsea cubeba essential oil, 0.04-0.8 parts of galangal essential oil, 0.64-12.8 parts of amomum villosum essential oil, and 0.32-6.4 parts of condiment osmanthus fragrans essential oil.

[0016] According to the application of the second aspect of the embodiment of the present invention, there is provided an oral care product, the oral care product comprising the above-mentioned anti-caries oral care composition containing patchouli essential oil.

[0017] According to the application of the embodiment of the present invention, at least the following beneficial effects are achieved: the composition formula of the present invention is not only limited to the application of toothpaste, but also shows wide applicability in a series of oral care products such as tooth powder, liquid toothpaste, mouthwash, tooth paste, chewing gum, oral freshening spray and tooth care gel. When preparing these products, it is only necessary to follow the conventional technical means in the industry, and scientifically integrate the specific composition of patchouli essential oil, cinnamon essential oil, Amomum villosum essential oil, etc. (which can be used alone or in combination) into the formula system of each product. Thanks to the composition of the present invention, the oral care product prepared shows excellent anti-caries performance, indicating that it has huge application potential and market value in the industrial field.

[0018] According to some embodiments of the present invention, the oral care product includes at least one of toothpaste, tooth powder, liquid toothpaste, mouthwash, tooth stickers, chewing gum, oral spray, and tooth gel.

[0019] According to some embodiments of the present invention, the oral care product is toothpaste, and the base material comprises a moisturizer, an abrasive, a thickener, a foaming agent, a sweetener, a flavor and a preservative.

[0020] According to some embodiments of the invention, the humectant comprises at least one of glycerol, propylene glycol, diethylene glycol, sorbitol or polyethylene glycol.

[0021] According to some embodiments of the invention, the abrasive comprises at least one of calcium carbonate, hydrated silica, silicon dioxide, calcium pyrophosphate or hydroxyapatite.

[0022] According to some embodiments of the present invention, the thickener includes at least one of sodium carboxymethyl cellulose, xanthan gum, carrageenan, nonionic guar gum, sodium alginate, hydroxyethyl cellulose or carbomer.

[0023] According to some embodiments of the invention, the foaming agent includes at least one of sodium lauryl sulfate, sodium lauroyl sarcosinate, sodium lauryl sulfoacetate or cocopropyl betaine.

[0024] According to some embodiments of the invention, the sweetener comprises at least one of saccharin sodium, monk fruit extract, xylitol or glycerol.

[0025] According to some embodiments of the invention, the flavor comprises at least one of menthol, peppermint oil, spearmint or cardamom.

[0026] According to some embodiments of the invention, the preservative comprises at least one of ethylparaben, propylparaben or sodium benzoate.

[0027] According to some embodiments of the present invention, the toothpaste comprises the following raw materials in parts by weight:

[0028] Composition 1.0625-21.24 parts

[0029] 70-90 parts of base material.

[0030] According to some embodiments of the present invention, the toothpaste comprises the following raw materials in parts by weight:

[0031] 1.0625-21.24 parts of anti-caries oral care composition

[0032] Moisturizer 10-70 parts

[0033] Friction agent 20-60 parts

[0034] Thickener 0.1-10 parts

[0035] Foaming agent 0.1-10 parts

[0036] Sweetener 0.1-2 parts

[0037] 0.1-5 parts of flavor

[0038] Preservative 0.1-1 part.

[0039] According to some embodiments of the present invention, the toothpaste comprises the following raw materials in parts by weight:

[0040] Patchouli essential oil 0.0025-0.04 parts

[0041] Cinnamon essential oil 0.04-0.8 parts

[0042] Litsea cubeba essential oil 0.02-0.4 parts

[0043] Kaempferia galanga essential oil 0.04-0.8 parts

[0044] Amomum villosum essential oil 0.64-12.8 parts

[0045] Seasoning: 0.32-6.4 parts of osmanthus essential oil

[0046] Moisturizer 10-70 parts

[0047] Friction agent 20-60 parts

[0048] Thickener 0.1-10 parts

[0049] Foaming agent 0.1-10 parts

[0050] Sweetener 0.1-2 parts

[0051] 0.1-5 parts of flavor

[0052] Preservative 0.1-1 part.

[0053] According to some embodiments of the present invention, the raw materials of the toothpaste further include water, and the total mass of the raw materials is 100 parts, with water being the balance.

[0054] According to the application of the second aspect of the present invention, there is also provided a method for preparing an oral care toothpaste, comprising the following steps:

[0055] S1, dispersing the thickener in the humectant to form a mixed solution A after swelling;

[0056] S2, adding a sweetener, a preservative and water to the mixed solution A to obtain a mixed solution B, and aging;

[0057] S3, adding a friction agent to the mixture B and mixing to obtain a mixture C;

[0058] S4, adding a foaming agent to the mixture C and mixing to obtain a mixture D;

[0059] S5, adding the anti-caries oral care composition containing patchouli essential oil to the mixture D, and mixing to obtain a mixture E;

[0060] S6. Add essence into the mixture F to obtain the product.

[0061] According to the preparation method of the embodiment of the present invention, at least the following beneficial effects are achieved: the toothpaste preparation process of the present invention is simple and has good production and application prospects. In the preparation process, the dispersion and swelling process of the thickener and the humectant are firstly carried out to build a stable structural framework for the toothpaste. This link ensures the basic stability of the toothpaste and lays a solid foundation for the integration of subsequent ingredients. Next, we introduce sweeteners, preservatives, abrasives and foaming agents into this basic system one by one according to specific process steps, and through a fine mixing process, ensure that these ingredients can be evenly integrated, thereby improving the sensory quality, safety and cleaning performance of the toothpaste. On the basis of this uniform mixing, we further carefully blend patchouli essential oil with other selected essential oils (which may include cinnamon essential oil, cassia bark essential oil, galangal essential oil, amomum essential oil and condiment osmanthus essential oil, etc.) to ensure that they can be evenly distributed in the toothpaste. The selection and proportioning of these essential oils are intended to enhance the aroma and taste of the toothpaste, and may bring additional oral health benefits, such as antibacterial, soothing the oral cavity or improving the oral environment. The preparation process of the scheme of the present invention not only ensures the physical stability of the toothpaste, such as fine texture and not easy to delaminate, but also maximizes the function of each component in the toothpaste, bringing consumers a toothpaste product that is both safe, reliable, efficient and practical.

[0062] According to some embodiments of the present invention, if the volatile oil is precipitated or difficult to dissolve, the volatile oil can be dissolved in a Tween 80 aqueous solution with a concentration of 0.05-1% and then added to the toothpaste.

[0063] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0065] Figure 1 The chromatogram of the raw material patchouli essential oil used in the embodiments of the present invention.

[0066] Figure 2 This is a schematic diagram of the effect of patchouli essential oil, a raw material used in an embodiment of the present invention, on the growth curve of Streptococcus mutans. DETAILED DESCRIPTION

[0067] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the present invention. The test methods used in the embodiments are conventional methods unless otherwise specified; the materials, reagents, etc. used, unless otherwise specified, can be reagents and materials obtained from commercial channels. Unless otherwise specified, the same parameter in each embodiment has the same value. The embodiments described below are exemplary and are only used to explain the present invention, and cannot be understood as limitations on the present invention.

[0068] In the description of the present invention, the description with reference to the term "some embodiments" or the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0069] In the description of the present invention, if there is a description of first, second, etc., it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0070] The patchouli essential oil and cinnamon essential oil, litsea cubeba essential oil, kaempferia galanga essential oil, amomum villosum essential oil, and spice osmanthus fragrans essential oil in the embodiments of the present invention can all be purchased through commercial means. The patchouli essential oil and kaempferia galanga essential oil used in the embodiments are extracted by supercritical carbon dioxide fluid; litsea cubeba essential oil, amomum villosum essential oil, and spice osmanthus fragrans essential oil are extracted by steam distillation, and cinnamon essential oil is purchased from Asus Spice Oil Co., Ltd. Among them, the extraction methods of litsea cubeba essential oil, amomum villosum essential oil, and spice osmanthus fragrans essential oil are all steam distillation methods routinely used in laboratories, with a solid-liquid ratio of 1:5 to 1:15.

[0071] The supercritical carbon dioxide extraction parameters used for patchouli essential oil were set as follows: extraction pressure of 30 MPa, extraction kettle temperature of 55 °C, separation kettle I extraction temperature of 40 °C, separation kettle II extraction temperature of 30 °C, CO2 flow rate of 20 L / h, and extraction time of 1.5 h.

[0072] The supercritical carbon dioxide extraction parameters used for galangal essential oil were set as follows: extraction kettle pressure of 22 MPa, extraction kettle temperature of 55°C, separation kettle I extraction temperature of 40°C, separation kettle II extraction temperature of 30°C, CO2 flow rate of 30 kg / h, and extraction time of 2 h.

[0073] Extraction method of patchouli essential oil:

[0074] The above-ground dry part of patchouli was collected in November, dried, crushed and passed through a 50-mesh sieve, and subjected to supercritical carbon dioxide extraction within 24 hours. The color of the extracted essential oil was yellow-green, and the oil yield was 2.6%. The obtained essential oil was sealed and stored in a refrigerator for future use.

[0075] The supercritical carbon dioxide extraction parameters used for the essential oil extraction are set as follows: extraction pressure of 30 MPa, extraction kettle temperature of 55° C., separation kettle I extraction temperature of 40° C., separation kettle II extraction temperature of 30° C., CO2 flow rate of 20 L / h, and extraction time of 1.5 h.

[0076] Qualitative and quantitative analysis of chemical components of patchouli essential oil:

[0077] 10 μL of patchouli essential oil was taken and placed in 990 μL of chromatographic grade n-hexane, mixed thoroughly, and filtered through a 0.22 μm organic filter membrane. The following GC-MS conditions were used for analysis.

[0078] Chromatographic conditions: The chromatographic column was a DB-5MS capillary column (30.00 m × 250.00 μm × 0.25 μm); the column temperature was programmed, with an initial temperature of 60 °C maintained for 2 min, and then 15 °C min -1 Raise to 150℃, hold for 2min, then increase to 3℃·min -1 Raise to 220℃, and finally at 20℃·min -1Raise to 300℃ and maintain for 5min; carrier gas is helium, column flow rate is 1.0mL·min -1 ; Injection volume 0.4μL, split injection, split ratio 30:1; purge flow 3.0mL·min -1 ; Chromatography-mass spectrometry interface temperature 260℃.

[0079] Mass spectrometry conditions: EI source, ion source temperature 230°C, electron energy 70eV, scan range 35-300m / z. Database is NIST2017.

[0080] Under the above GC-MS conditions, the chromatogram of patchouli essential oil is obtained as follows: Figure 1 As shown, the chemical components and relative contents were analyzed by combining computer spectrum retrieval qualitative identification and peak area normalization method. The specific results are shown in Table 1.

[0081] Table 1 GC-MS chemical components and relative contents of patchouli essential oil

[0082]

[0083]

[0084]

[0085]

[0086] From Table 1 and Figure 1 It can be seen that a total of 50 compounds were identified in patchouli essential oil, among which the components with higher content were patchouli alcohol (27.95%), γ-cedrene (12.36%), and α-guaiacene (11.26%).

[0087] The "room temperature" referred to in the present invention refers to 25±5°C, and is specifically 25°C in the embodiments.

[0088] Example 1

[0089] This example provides an anti-caries oral care composition containing patchouli essential oil, which is composed of the following components in percentage by weight: 0.02 parts of patchouli essential oil and 0.4 parts of cinnamon essential oil.

[0090] This example also provides the use of the above-mentioned dental caries-preventing oral care composition in the preparation of toothpaste, and the raw materials for the preparation also include abrasives, thickeners, moisturizers, sweeteners, foaming agents, preservatives, essences and water. The weight percentages of each raw material are respectively: 0.42 parts of dental caries-preventing oral care agent (0.02 parts of patchouli essential oil, 0.4 parts of cinnamon essential oil), 60 parts of moisturizer (composed of glycerin and sorbitol, 20 parts and 40 parts respectively), 22 parts of abrasive (specifically silicon dioxide), 1 part of thickener (composed of non-ionic guar gum and sodium carboxymethyl cellulose, 0.75 parts and 0.25 parts respectively), 2 parts of foaming agent (specifically sodium lauryl sulfate), 0.35 parts of sweetener (specifically sodium saccharin), 1 part of essence (specifically peppermint essential oil), 0.15 parts of preservative (specifically ethyl paraben), the total raw material mass fraction is 100 parts, and water is the balance.

[0091] Specifically, the preparation process is as follows:

[0092] S1: Disperse the thickener evenly in the moisturizer and form a mixed solution A after fully swelling;

[0093] S2: adding sweetener, preservative and water to the mixed solution A, stirring evenly to obtain the mixed solution B, and then fully aging;

[0094] S3: Add the friction agent to the mixture B and mix thoroughly to obtain the mixture C;

[0095] S4: adding a foaming agent to the mixture C and mixing thoroughly to obtain a mixture D;

[0096] S5: adding the anti-caries oral care composition containing patchouli essential oil to the mixture D and mixing well to obtain a mixture E;

[0097] S6: adding essence to the mixed material F, homogenizing and degassing to obtain the product.

[0098] Example 2

[0099] This example provides an anti-caries oral care composition and toothpaste, which differs from Example 1 in that the total weight of the composition is 6.02 parts, and specifically consists of the following components in parts by weight: 0.02 parts of patchouli essential oil and 6 parts of Amomum villosum essential oil.

[0100] Example 3

[0101] This example provides an anti-caries oral care composition and toothpaste, which differs from Example 1 in that the total weight of the composition is 4.521 parts, and is specifically composed of the following components in parts by weight: 0.01 parts of patchouli essential oil, 0.2 parts of cinnamon essential oil, 3 parts of amomum villosum essential oil, and 1.5 parts of condiment osmanthus fragrans essential oil.

[0102] Example 4

[0103] This example provides an anti-caries oral care composition and toothpaste, which differs from Example 1 in that the total weight of the composition is 5.11 parts, and is specifically composed of the following components in parts by weight: 0.01 parts of patchouli essential oil, 0.2 parts of cinnamon essential oil, 0.2 parts of litsea cubeba essential oil, 0.2 parts of galangal essential oil, 3 parts of amomum villosum essential oil, and 1.5 parts of condiment osmanthus fragrans essential oil.

[0104] Comparative Example 1

[0105] This example provides a toothpaste, which differs from Example 1 in that the composition is replaced with 0.0025 parts of patchouli essential oil.

[0106] Comparative Example 2

[0107] This example provides a toothpaste, which differs from Example 1 in that the composition is replaced with 0.02 parts of patchouli essential oil.

[0108] Comparative Example 3

[0109] This example provides an anti-caries oral care composition and toothpaste, which differs from Example 1 in that the total weight of the composition is 0.22 parts, and specifically consists of the following components in parts by weight: 0.02 parts of patchouli essential oil and 0.2 parts of litsea cubeba essential oil.

[0110] Comparative Example 4

[0111] This example provides an anti-caries oral care composition and toothpaste, which differ from Example 1 in that the total weight of the composition is 0.42 parts, and specifically consists of the following components in parts by weight: 0.02 parts of patchouli essential oil and 0.4 parts of kaempferia galanga essential oil.

[0112] Comparative Example 5

[0113] This example provides an anti-caries oral care composition and toothpaste, which differs from Example 1 in that the total weight of the composition is 3.02 parts, and is specifically composed of the following components in parts by weight: 0.02 parts of patchouli essential oil and 3 parts of condiment osmanthus essential oil.

[0114] The toothpaste formulas in the embodiments and comparative examples are specifically shown in Table 2 below:

[0115] Table 2 Antibacterial and anti-caries toothpaste formula (unit: mass percentage)

[0116]

[0117]

[0118] The total mass percentage of toothpaste is 100%. When the sum of the mass percentages of other toothpaste raw materials is less than 100%, the remainder is supplemented with water.

[0119] Test Case

[0120] This example tests the anti-caries effect of the toothpaste. The specific operation is to take the toothpaste prepared in the above embodiment and comparative example and test its antibacterial effect on Streptococcus mutans.

[0121] 1) Preparation of Streptococcus mutans suspension

[0122] The activated standard strain of Streptococcus mutans (ATCC 25175, commercially available) was picked and inoculated on the RCA plate and evenly spread, then inverted and placed in an anaerobic bag. After culturing in a 37°C incubator for 24 hours, the bacterial moss was scraped from the plate with a disposable sterile inoculation loop, suspended in sterile PBS to prepare a bacterial solution, and streaked on the RCA plate for culturing. After anaerobic culture for 24 hours, a single colony was picked and anaerobically cultured in a BHI liquid culture medium at 37°C and 180 rpm in a shaking incubator; the bacteria in the logarithmic growth period were taken and the absorbance (OD) was adjusted to 1.0 at a wavelength of 600 nm using an ultraviolet spectrophotometer, and used as the standard bacterial solution for later use.

[0123] 2) Patchouli essential oil growth curve test on Streptococcus mutans

[0124] The experimental group diluted patchouli essential oil to MIC and below (1 / 2MIC, 1 / 4MIC, 1 / 8MIC) three concentrations, took 1mL and added to the test tubes, and then added 1mL of working bacterial solution diluted 100 times with the standard bacterial solution, and the bacterial solution control group added 0.1% Tween 80 solution and bacterial solution of the same concentration as the MIC group. Take out one tube at 0, 4, 8, 12, 24, 48h, measure OD600 value, and draw growth curve.

[0125] Growth curve Figure 2 As shown in the figure, compared with the control group, patchouli essential oil can significantly inhibit the time for Streptococcus mutans to reach the plateau phase. The control group entered the logarithmic growth phase at 4h and the plateau phase at 12h; the MIC group and the 1 / 2MIC group entered the logarithmic growth phase at 12h and the plateau phase at 24h; the 1 / 4MIC group and the 1 / 8MIC group entered the logarithmic growth phase at 8h and the plateau phase at 24h. Compared with the control group, patchouli essential oil can significantly inhibit the time for Streptococcus mutans to enter the logarithmic growth phase.

[0126] 3) Combined drug sensitivity test of patchouli essential oil and other active ingredients

[0127] Using Streptococcus mutans as the indicator bacterium, the minimum inhibitory concentrations (MICs) of Pogostemon cablin essential oil, Cinnamomum cassia essential oil, Litsea cubeba essential oil, Kaempferia galanga essential oil, Amomum villosum essential oil, and Murraya paniculata (L.) Jack essential oil used as seasonings were determined. The MICs of Pogostemon cablin essential oil and various compounds were diluted by 2, 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, and 1 / 32 times using the checkerboard method with BHI medium for a 7×7 combination test. The bacterial suspension concentration was set the same as in the part of the test for determining the growth curve of Pogostemon cablin essential oil against Streptococcus mutans. The optimal combined effect concentration was selected, and the fractional inhibitory concentration index (FICI) was calculated.

[0128] The calculation formula for FICI is as follows:

[0129]

[0130] Judgment criteria: FICI ≤ 0.5, synergistic effect; 0.5 < FICI ≤ 1, additive effect; 1 < FICI ≤ 2, no effect; FICI > 2, antagonistic effect.

[0131] The test results showed that the MIC value of Pogostemon cablin essential oil was 100 μg / ml, the MIC value of Cinnamomum cassia essential oil was 1.6 mg / ml, the MIC value of Litsea cubeba essential oil was 800 μg / ml, the MIC value of Kaempferia galanga essential oil was 1.6 mg / ml, the MIC value of Amomum villosum essential oil was 25.6 mg / ml, and the MIC value of Murraya paniculata (L.) Jack essential oil used as a seasoning was 12.8 mg / ml.

[0132] The combined drug sensitivity test showed that:

[0133] For Pogostemon cablin essential oil and magnolol, the optimal combined effect concentration was Pogostemon cablin essential oil at 1 / 16 MIC concentration and magnolol at 1 / 4 MIC concentration, and the FICI value was 0.3125, indicating that the combined use of Pogostemon cablin essential oil and magnolol had a synergistic effect on the antibacterial effect against Streptococcus mutans.

[0134] For Pogostemon cablin essential oil and bornyl acetate, the optimal combined effect concentration was Pogostemon cablin essential oil at 1 / 16 MIC concentration and bornyl acetate at 1 / 4 MIC concentration, and the FICI value was 0.3125, indicating that the combined use of Pogostemon cablin essential oil and bornyl acetate had a synergistic effect on the antibacterial effect against Streptococcus mutans.

[0135] For Pogostemon cablin essential oil and citral, the optimal combined effect concentration was Pogostemon cablin essential oil at MIC concentration and citral at MIC concentration, and the FICI value was 2, indicating that the combined use of Pogostemon cablin essential oil and citral had no effect on the antibacterial effect against Streptococcus mutans.

[0136] For patchouli essential oil and ethyl p-methoxycinnamate, the optimal combined effect concentration was 1 / 2MIC concentration of patchouli essential oil and MIC concentration of ethyl p-methoxycinnamate, and the FICI value was 1.5, indicating that the combined use of patchouli essential oil and ethyl p-methoxycinnamate had no effect on the antibacterial effect of Streptococcus mutans.

[0137] For patchouli essential oil and β-caryophyllene, the optimal combined effect concentration was 1 / 2MIC concentration of patchouli essential oil and 1 / 4MIC concentration of β-caryophyllene, and the FICI value was 0.75, indicating that the combined use of patchouli essential oil and β-caryophyllene had an additive effect on the antibacterial effect of Streptococcus mutans.

[0138] 4) Evaluation test of the antibacterial effect of compound antibacterial and anti-caries toothpaste containing patchouli essential oil

[0139] The antibacterial effect of the toothpaste in the examples and comparative examples was investigated by referring to "5.1.2 Carrier Immersion Quantitative Antibacterial Test" in the "Health Industry Standards of the People's Republic of China". Mutans Streptococcus was diluted with PBS to about 5×10 6 CFU / mL~5×10 7 CFU / mL is used to make a bacterial suspension for later use. Take 10μL of the bacterial suspension on a drug-sensitive paper, dry it at 36℃±1℃ or air it at room temperature for later use. Weigh 5g / piece of the toothpaste sample prepared in the embodiment or comparative example into a sterile plate, place it in a 20℃±1℃ water bath for 5min, take the above-mentioned drug-sensitive paper with sterile tweezers, and immerse the carrier completely in the sample, and time it for 5min. Then take out the drug-sensitive paper and add it to a 5.0mL PBS test tube, mix it, oscillate it, wash off the test bacteria, absorb 1.0mL of the sample solution, and determine the number of surviving bacteria according to the live bacteria culture counting method. Inoculate 2 plates with each tube of sample solution. If the number of colonies growing on the plate is large, PBS can be used for 10-fold serial dilution, and then live bacteria culture counting can be performed. Take 10.0g of blank toothpaste matrix that does not contain antibacterial and anti-caries active ingredients and soak it in 2 drug-sensitive paper sheets for parallel experiments as a positive control. The amount of bacteria recovered in the positive control is 1.0×10 4 CFU / piece~9.0×10 4 CFU / piece. Take PBS and culture medium from the same batch as negative control. All test samples and control samples were cultured at 36℃±1℃, and the results were observed for 24 hours. The test was repeated 3 times and the inhibition rate was calculated.

[0140] The antibacterial rate calculation method is as follows:

[0141]

[0142] Where:

[0143] X—inhibition rate, %;

[0144] A0—average colony count of control samples, in CFU / piece;

[0145] A1—Average colony count of the tested samples, in CFU / piece.

[0146] The test results are shown in Table 3.

[0147] Table 3 Test results of antibacterial rate of toothpaste against Streptococcus mutans prepared in Examples and Comparative Examples

[0148] Toothpaste No. Antibacterial rate (%) Example 1 87.2±0.1 Example 2 86.6±0.2 Example 3 98.6±0.4 Example 4 99.5±0.2 Comparative Example 1 55.0±0.3 Comparative Example 2 72.7±0.5 Comparative Example 3 75.2±0.6 Comparative Example 4 70.2±0.5 Comparative Example 5 82.4±0.3

[0149] If the inhibition rate is ≥50% to 90%, it is judged to have an antibacterial effect; if the inhibition rate is ≥90%, it is judged to have a strong antibacterial effect.

[0150] The test results show that the embodiments of the present invention all have antibacterial effects, especially when multiple components are compounded together, the effect can be significantly improved to more than 99.5%.

[0151] 5) Toothpaste quality evaluation

[0152] The quality of the antibacterial and anti-caries toothpaste containing patchouli essential oil prepared by the method of the embodiment was tested. With reference to the sensory and physical and chemical indicators in GB / T8372-2017 Toothpaste, the quality was evaluated by using the paste morphology, stability and foam volume as a comprehensive evaluation method.

[0153] Evaluation of forming condition and paste morphology: Take a toothpaste sample, squeeze 2cm from a 8mm diameter mouth onto a toothbrush. The toothpaste prepared in this embodiment is easy to squeeze out, has obvious wire drawing, is easy to cut, and has no obvious collapsed area; the paste is clean and delicate, with uniform color, bright and free of impurities.

[0154] Stability evaluation: Take 2 toothpaste samples, store one sample at room temperature; put the other sample into a refrigerator at (-8±1)℃ for 8 hours, then take it out, and then put it into a constant temperature incubator at (45±1)℃ for 8 hours, then take it out, restore it to room temperature, open the cover, the paste should not overflow from the tube, turn the toothpaste tube upside down, and no liquid should drip from the tube within 10 seconds; compare the paste with the room temperature sample after squeezing it out. The toothpaste prepared in this embodiment does not overflow from the tube, does not separate liquid, and the fragrance body has normal color.

[0155] Foam volume evaluation: Pre-weigh 45°C, 20mL water, weigh 1g of the toothpaste to be tested in a beaker, add a small amount of water to dissolve the paste, pour the dissolved paste into a stoppered measuring cylinder, wash the beaker with the remaining water, pour it into the measuring cylinder, cover and press the stopper tightly, shake the solution in the measuring cylinder with the maximum force, stop after the paste is completely dissolved, place the measuring cylinder on the operating table and let it stand for 5 minutes, record the foaming amount based on the most concentrated position of the foam distribution in the measuring cylinder, repeat 3 times, and take the average value. The toothpaste foam volume prepared in this embodiment is ≥90mL, which meets the corresponding national standards. The toothpaste foam volume prepared in each embodiment of the present invention is ≥90mL, which meets the corresponding national standards.

[0156] The mechanism of action of each component in the anti-caries oral care composition of the present invention is speculated as follows:

[0157] Patchouli essential oil is a plant essence derived from nature. With its unique and charming aroma, it is praised as the "daughter red of the essential oil world" in the field of essential oils and is widely regarded as a model of oriental fragrance. The reason why this essential oil is highly respected is not only because of its charming aroma, but also because of its multiple pharmacological activities. Scientific research has confirmed that patchouli essential oil has excellent antibacterial and anti-pathogenic microbial properties, as well as significant anti-inflammatory ability, which makes it widely used in many fields such as food, medicine, cosmetics and personal care products. Specifically, patchouli essential oil can effectively inhibit the growth and reproduction of more than 20 fungi, including but not limited to Aspergillus oryzae, various yeasts, Penicillium and dermatophytes, showing its extensive inhibitory effect on fungal microorganisms. At the same time, it can also fight more than 30 kinds of bacteria, such as common Staphylococcus aureus, Helicobacter pylori, Escherichia coli, Shigella dysenteriae, etc., showing a strong antibacterial spectrum. These findings further confirm the potential of patchouli essential oil in maintaining health and preventing infection. It is particularly worth mentioning that the scheme of the present invention deeply explores the inhibitory effect of patchouli essential oil on Streptococcus mutans. Streptococcus mutans is one of the main bacteria that cause dental caries, and the research results show that patchouli essential oil has a good inhibitory effect on Streptococcus mutans. This discovery provides a scientific basis for the application of patchouli essential oil in oral care products, indicating that incorporating it into oral care products such as toothpaste and mouthwash may achieve an ideal antibacterial and anti-caries effect, opening up a new way to improve oral health and prevent dental caries.

[0158] As a plant with a long history of medicinal and edible properties, cinnamon is widely used in the food industry. Its rich chemical components constitute the unique pharmacological and edible value of cinnamon, including but not limited to volatile oils, flavonoids, polysaccharides, terpenes, lignans, coumarins and various minerals. Among these components, volatile oils are the main active components of cinnamon essential oil, which gives cinnamon its iconic aroma and efficacy. The aroma of cinnamon essential oil has both the sweetness and warmth of cinnamon bark, and combines the layers of woody, creamy, green and fruity aromas. The aroma is rich and lasting. This unique aroma is not only pleasant, but also contains significant antibacterial activity, which lays a solid foundation for the application of cinnamon essential oil in the field of health and care. Current scientific research shows that cinnamon essential oil has excellent efficacy in maintaining oral health. It can effectively inhibit the formation of dental plaque, and this effect has been verified in clinical treatment, showing a strong inhibitory effect on the growth and reproduction of dental plaque bacteria. This broad-spectrum antibacterial effect is not limited to specific strains of bacteria, but has an inhibitory effect on a variety of oral bacteria, thus helping to maintain a clean and healthy oral environment. In addition, cinnamon essential oil is also popular for its good taste and fresh smell. In the application of oral care products, it can not only effectively improve the user experience, but also because of its natural ingredients and long-term safety, no significant side effects have been reported. Therefore, as a natural, safe and efficient oral care ingredient, cinnamon essential oil has broad application prospects and provides new ideas and solutions for oral health care.

[0159] As a traditional Chinese medicine with a long history of medicinal use, Amomum villosum is widely used in the field of traditional Chinese medicine and is an important ingredient in many Chinese patent medicines and prescriptions. According to statistics, there are more than 300 Chinese patent medicines with Amomum villosum as the main ingredient or adjuvant, and more than 1,000 related prescriptions. These prescriptions are mainly used to treat or regulate TCM syndromes such as replenishing qi, digesting food and eliminating accumulation, and protecting the fetus. Amomum villosum not only occupies an important position in traditional medicine, but is also a good product for both medicine and food, and is widely used in daily diet and diet therapy. The unique efficacy of Amomum villosum is mainly attributed to its rich volatile oil components, which is the key to the pharmacological activity of Amomum villosum. Amomum villosum volatile oil not only shows a significant inhibitory effect on a variety of fungi, but also shows strong antibacterial ability against bacteria. Scientific research shows that Amomum villosum volatile oil can effectively inhibit the growth and reproduction of a variety of common bacteria including Staphylococcus aureus, Salmonella, Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella pneumoniae. Among these bacteria, there are common food-borne pathogens and common bacteria that cause hospital-acquired infections. The inhibitory effect of Amomum villosum volatile oil on them further confirms the potential value of Amomum villosum in antibacterial, anti-inflammatory and infection prevention. Therefore, as a natural product with both medicinal and edible value, the pharmacological activity of its volatile oil components in antibacterial and anti-inflammatory aspects provides a broad application prospect for Amomum villosum in traditional Chinese medicine, food health care, and prevention and treatment of infectious diseases.

[0160] Litsea cubeba, also known as Litsea cubeba, Costus odoratus or Litsea cubeba, is a precious plant spice resource unique to China. It has attracted widespread attention for its unique pharmacological properties, and has shown good therapeutic effects on relieving vomiting symptoms caused by stomach cold and treating diseases such as rheumatism and bone pain. In daily life applications, Litsea cubeba is often used as a condiment in cooking to add flavor to food. It is also used in the brewing of tea to add a unique aroma and health benefits to the drink. Furthermore, the essential oil extracted from Litsea cubeba is a non-toxic, light yellow oily liquid. This essential oil has excellent antibacterial activity. Scientific research has shown that it exhibits significant inhibitory effects on a variety of bacteria, fungi and pathogenic microorganisms, showing its great potential in the field of natural antibacterial agents.

[0161] Kaempferia galanga, also known as sand ginger, Sannai or mountain pepper, is a perennial herb with rhizomes widely used in traditional medicine and cooking. As an aromatic stomachic, the rhizomes of Kaempferia galanga are used to treat a variety of symptoms, including dispelling cold, removing dampness, warming the spleen and stomach, and removing bad air. Its unique aroma and medicinal value make it an important ingredient in many traditional prescriptions. At the same time, Kaempferia galanga is also used as a seasoning spice because of its unique aroma, adding a unique flavor to food. The monomer compounds and volatile oil components in Kaempferia galanga have attracted much attention due to their significant biological activity and show great development potential. Specifically, the volatile oils and monomer compounds of Kaempferia galanga have excellent anti-inflammatory and antioxidant performance, can effectively reduce inflammatory responses, fight free radical damage, and thus protect cells from oxidative stress. In addition, studies have also shown that Kaempferia galanga has potential anti-cancer and anti-cancer effects, can inhibit the growth and spread of cancer cells, and provides new ideas for cancer prevention and treatment. What is even more surprising is that Kaempferia galanga also shows a killing effect on nematodes, which also has important application prospects in agricultural pest control.

[0162] Osmanthus fragrans, belonging to the genus Osmanthus of the Rutaceae family, is a plant with multiple application values. Its aliases include hemp leaf and curry leaf. In many countries in Southeast Asia, Osmanthus fragrans is often used as a seasoning ingredient for foods such as curry due to its unique fragrance, greatly enriching the flavor level of local food culture. In traditional folk medicine, Osmanthus fragrans is endowed with sedative and anti-inflammatory functions. These applications to a certain extent reflect people's initial understanding of its medicinal value. With the in-depth research of modern pharmacology, more health benefits of Osmanthus fragrans have been revealed: its roots have been proven to have analgesic, insect repellent, anti-inflammatory and memory-enhancing effects; while the bark and stems have the effect of refreshing the brain and helping to improve mental state. In terms of external treatment, Osmanthus fragrans also shows a wide range of application potential. Its medicinal materials can be used to treat trauma caused by insect bites, snake bites, etc., and effectively relieve related symptoms. In addition, the leaves of Murraya paniculata have also shown certain efficacy in treating dysentery and rashes, while the extract of dried leaves is used as a natural medicine to prevent and treat vomiting, providing a new option for relieving digestive system discomfort.

[0163] In the anti-caries oral care composition of the present scheme, its effective ingredients carefully select patchouli essential oil and cinnamon essential oil or amomum essential oil (one or two of which can be used in combination), and can be further compounded to add Litsea cubeba essential oil, Kaempferia galanga essential oil or seasoning Osmanthus fragrans essential oil (one or more of which can be compounded). In strict antibacterial experiments, the antibacterial composition showed a significant inhibitory effect on the main pathogenic bacteria causing dental caries, Streptococcus mutans. This discovery not only verifies the effectiveness of the composition, but also lays a solid foundation for its application in the field of oral care. All essential oil components selected by the present invention are derived from natural plant extracts, which not only have a fresh smell, but also have low cost and a wide range of sources. More importantly, these ingredients all have a high degree of biosafety and are harmless to the human body. This feature makes the antibacterial composition have unique advantages in the development of oral care products.

[0164] The solution of the present invention provides a new solution to the problems of current anti-caries oral care products, such as dental fluorosis and the selectivity of fluoride action sites. This solution not only has healthy ingredients and scientific formula, but also can effectively fight bacteria and reduce inflammation, and inhibit the occurrence of dental plaque and caries for a long time. This innovative application not only provides new ideas for the development of oral care products, but also provides a safer and more effective means for solving oral health problems. Therefore, the solution of the present invention has broad market application prospects and is worthy of further promotion and in-depth research.

[0165] In summary, the embodiments of the present invention provide an anti-caries oral care composition containing patchouli essential oil, and the oral care product prepared by the composition has multiple advantages: its formula design is scientific, the raw material source is wide, it is safe and reliable to use, and it emits a fresh smell. In addition, the composition exhibits excellent biological safety, can effectively inhibit the growth of dental plaque, maintain oral hygiene and hygiene for a long time, and exerts a significant antibacterial and antibacterial effect, thereby refreshing breath and maintaining oral health.

[0166] The embodiments of the present invention are described in detail above, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. An anti-caries oral care composition containing patchouli essential oil, characterized in that: The composition further comprises at least one of cinnamon essential oil or amomum essential oil.

2. The anti-caries oral care composition according to claim 1, characterized in that: The composition also includes at least one of Litsea cubeba essential oil, Kaempferia galanga essential oil or spices Osmanthus fragrans essential oil.

3. The anti-caries oral care composition according to claim 1, characterized in that: The weight proportion of patchouli essential oil in the composition is 6-99.9%.

4. The anti-caries oral care composition according to claim 1, characterized in that: The composition contains patchouli essential oil, cinnamon essential oil, litsea cubeba essential oil, kaempferia galanga essential oil, amomum villosum essential oil and spice osmanthus cassia oil, and the mass ratio of the patchouli essential oil, cinnamon essential oil, litsea cubeba essential oil, kaempferia galanga essential oil, amomum villosum essential oil and spice osmanthus cassia oil is 1:16-20:8-20:16-20:256-320:128-160.

5. The anti-caries oral care composition according to claim 1, characterized in that: The composition comprises the following raw materials in parts by weight: 0.0025-0.04 parts of patchouli essential oil, 0.04-0.8 parts of cinnamon essential oil, 0.02-0.4 parts of litsea cubeba essential oil, 0.04-0.8 parts of galangal essential oil, 0.64-12.8 parts of amomum villosum essential oil and 0.32-6.4 parts of condiment osmanthus fragrans essential oil.

6. An oral care product, characterized in that: The product comprises the anti-caries oral care composition according to any one of claims 1 to 5.

7. The oral care product according to claim 6, characterized in that: The oral care product includes at least one of toothpaste, tooth powder, liquid toothpaste, mouthwash, tooth stickers, chewing gum, oral spray, and tooth gel.

8. The oral care product according to claim 7, characterized in that: The oral care product is toothpaste, which comprises the following raw materials in parts by weight: 1.0625-21.24 parts of anti-caries oral care composition Moisturizer 10-70 parts Friction agent 20-60 parts Thickener 0.1-10 parts Foaming agent 0.1-10 parts Sweetener 0.1-2 parts 0.1-5 parts of flavor Preservative 0.1-1 part.

9. The oral care product according to claim 8, characterized in that: The toothpaste comprises the following raw materials in parts by weight: Patchouli essential oil 0.0025-0.04 parts Cinnamon essential oil 0.04-0.8 parts Litsea cubeba essential oil 0.02-0.4 parts Kaempferia galanga essential oil 0.04-0.8 parts Amomum villosum essential oil 0.64-12.8 parts Seasoning: 0.32-6.4 parts of osmanthus essential oil Moisturizer 10-70 parts Friction agent 20-60 parts Thickener 0.1-10 parts Foaming agent 0.1-10 parts Sweetener 0.1-2 parts 0.1-5 parts of flavor Preservative 0.1-1 part.

10. A method for preparing an oral care toothpaste, characterized in that: The steps include: S1, dispersing the thickener in the humectant to form a mixed solution A after swelling; S2, adding a sweetener, a preservative and water to the mixed solution A to obtain a mixed solution B, and aging; S3, adding a friction agent to the mixture B and mixing to obtain a mixture C; S4, adding a foaming agent to the mixture C and mixing to obtain a mixture D; S5. Adding the anti-caries oral care composition containing patchouli essential oil according to any one of claims 1 to 5 into the mixture D to obtain a mixture E; S6. Add essence into the mixture F to obtain the product.

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