A red thread traditional Chinese medicine composition for treating chronic obstructive pulmonary disease and its preparation method and use

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and in particular to a red thread Chinese medicine composition for treating chronic obstructive pulmonary disease, and its preparation method and use. The red thread Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-20 parts of Rhodiola rosea, 10-15 parts of Astragalus, 5-10 parts of Epimedium, 5-10 parts of Red Peony Root, 5-10 parts of Scrophularia, 5-10 parts of Golden Fagopyrum, 5-10 parts of Scutellaria, 10-15 parts of Licorice, 20-25 parts of Momordica grosvenori, and 30-40 parts of red thread. In the present invention, red thread, a characteristic medicinal material of Lingnan, is used as one of the raw materials, which produces a synergistic effect with Momordica grosvenori to improve the overall therapeutic effect of the Chinese medicine composition on mice with chronic obstructive pulmonary disease. Specifically, it can effectively reduce the degree of pulmonary obstruction, improve the ability of lung expansion, and improve the tissue elasticity of the lung.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a red thread silk traditional Chinese medicine composition for treating chronic obstructive pulmonary disease, and a preparation method and use thereof. Background Art

[0002] Chronic obstructive pulmonary disease (COPD) is abbreviated as chronic obstructive pulmonary disease (COPD), which refers to chronic bronchitis and emphysema with airflow obstruction. This disease is a common chronic disease in the respiratory department and can further develop into cor pulmonale and respiratory failure. Its mortality and disability rates are both high. According to statistics, the incidence of COPD in people aged ≥40 years worldwide is about 9% to 10%. Traditional Chinese medicine believes that COPD belongs to the category of "cough", "asthma" and "lung distension", which is mainly caused by long-term cough and asthma, delayed treatment, and damage to the lungs, spleen, and kidneys. Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and complications can have a significant impact on the severity of the patient's overall disease.

[0003] The TCM syndrome types of COPD include basic syndromes and common clinical syndromes. The basic syndromes of COPD include blood stasis syndrome, cold and fluid syndrome, phlegm-heat syndrome, phlegm-dampness syndrome, lung yin deficiency syndrome, lung qi deficiency syndrome, spleen qi deficiency syndrome, kidney qi deficiency syndrome, and kidney yin deficiency syndrome. Although the basic syndromes of COPD can appear alone, multiple syndromes often coexist. The common clinical syndromes of COPD include the following three categories: 1) Excessive syndrome, including external cold and internal fluid syndrome, wind-cold attacking the lungs syndrome, phlegm-dampness blocking the lungs syndrome, phlegm-heat blocking the lungs syndrome, and phlegm covering the mind and orifices syndrome. 2) Concomitant syndrome, mainly blood stasis syndrome. 3) Deficiency syndrome, including lung qi deficiency syndrome, lung-kidney qi deficiency syndrome, lung-kidney qi-yin deficiency syndrome, and lung-spleen qi deficiency syndrome. The TCM syndrome differentiation of the condition of patients with acute COPD is mainly based on excess syndrome, while the TCM syndrome differentiation of the condition of patients with stable COPD is mainly based on deficiency syndrome.

[0004] Hongjing Qingfei Recipe is an empirical prescription for the clinical treatment of exogenous diseases with phlegm-heat obstructing the lungs. It was finally modified after many clinical experiments. The original prescription contains 12 Chinese medicines. After research and simplification, four Chinese medicines with the same origin of medicine and food are selected: Rhodiola rosea, golden buckwheat, Scrophularia ningpoensis, and Momordica grosvenori. In the prescription, Rhodiola rosea is used as the main ingredient to strengthen the body and eliminate evil, protect the lung qi, and clear the exogenous evil; Golden buckwheat is used as the assistant to clear heat and detoxify, and clear the meridians of the lungs; Scrophularia ningpoensis is used as the assistant to nourish the yin of the lungs and moisten dryness; Momordica grosvenori is used to clear the throat and benefit the throat. The combination of the four medicines takes into account both strengthening the body and eliminating evil, which can not only clear the exogenous evil toxins, clear the internal evil toxins, but also replenish qi and nourish yin, and protect the positive energy. The results of many studies have shown that Hongjing Qingfei Recipe can relieve the symptoms of PM2.5 on respiratory inflammation and lung damage.

[0005] At present, there is no research report on the application of Hongjing Qingfei Fang in the treatment of stable chronic pulmonary obstructive pulmonary disease. There is also an urgent need for a Chinese patent medicine that can be used in the stable stage of chronic pulmonary obstructive pulmonary disease to improve the convenience and safety of application for patients or doctors. Summary of the invention

[0006] The purpose of the present invention is to provide a red thread silk traditional Chinese medicine composition for treating chronic obstructive pulmonary disease and a preparation method and use thereof.

[0007] To achieve the above objectives, the present invention provides a red thread traditional Chinese medicine composition for treating chronic obstructive pulmonary disease, wherein the red thread traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-20 parts of Rhodiola rosea, 10-15 parts of Astragalus, 5-10 parts of Epimedium, 5-10 parts of Red Peony Root, 5-10 parts of Scrophularia ningpoensis, 5-10 parts of Fagopyrum scabra, 5-10 parts of Scutellaria baicalensis, 10-15 parts of Licorice, 20-25 parts of Momordica grosvenori, and 30-40 parts of red thread.

[0008] Preferably, the red thread traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of Rhodiola rosea, 10 parts of Astragalus, 10 parts of Epimedium, 10 parts of Red Peony Root, 10 parts of Scrophularia ningpoensis, 10 parts of Fagopyrum scabra, 10 parts of Scutellaria baicalensis, 15 parts of Licorice, 25 parts of Momordica grosvenori, and 35 parts of red thread.

[0009] Preferably, the red thread is an ethanol extract of red thread.

[0010] Preferably, the preparation method of the ethanol extract of the red silk thread is: weigh the red silk thread, crush it, add ethanol with a mass concentration of 75% at a mass ratio of 1g: 5-7ml, heat and reflux at 80°C for 1.5-2h, filter the ethanol extract, recover the residue, add ethanol with a mass concentration of 75% at a mass ratio of 1g: 5-7ml, continue to heat and reflux for 1.5-2h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract.

[0011] The present invention also provides a method for preparing a red thread silk Chinese medicine composition for treating chronic obstructive pulmonary disease, the preparation method comprising the following steps:

[0012] (1) Weigh the red silk thread, crush it, add 75% ethanol at a mass ratio of 1g:5-7ml, heat and reflux at 80°C for 1.5-2h, filter the alcohol extract, recover the residue, add 75% ethanol at a mass ratio of 1g:5-7ml, continue to heat and reflux for 1.5-2h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract;

[0013] (2) respectively taking rhodiola rosea, astragalus, epimedium, red peony root, golden buckwheat, monk fruit, Scrophularia ningpoensis, golden buckwheat, and liquorice, crushing and mixing, adding 10 to 15 times the weight of water, heating and extracting at 95 to 100° C. for 45 to 60 minutes, filtering, and setting the filtrate aside; taking the filter residue, adding 8 to 10 times the weight of water, heating and extracting at 95 to 100° C. for 20 to 30 minutes, filtering, combining the filtrates obtained twice, and concentrating to a thick paste;

[0014] (3) The red thread silk extract obtained in step (1) and the thick paste obtained in step (2) are stirred and mixed to obtain a product.

[0015] The present invention also provides a use of a red thread Chinese medicine composition in the preparation of a drug for treating chronic obstructive pulmonary disease. The red thread Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-20 parts of Rhodiola rosea, 10-15 parts of Astragalus, 5-10 parts of Epimedium, 5-10 parts of Red Peony Root, 5-10 parts of Scrophularia, 5-10 parts of Golden Fagopyrum, 5-10 parts of Scutellaria, 10-15 parts of Licorice, 20-25 parts of Momordica grosvenori, and 30-40 parts of red thread.

[0016] Rhodiola rosea is cold, sweet, and astringent in nature and flavor. It belongs to the lung meridian and has the effects of replenishing qi and clearing the lungs, improving intelligence and nourishing the heart, and astringing and stopping bleeding. It has significant effects on treating qi deficiency and weakness, fear of cold after illness, shortness of breath and fatigue, lung heat cough, and hemoptysis. Fagopyrum strychnifolium is cold in nature, sour and bitter in taste, and belongs to the lung, stomach, and liver meridians. It has the functions of clearing heat and detoxifying, promoting blood circulation and eliminating carbuncle; dispelling wind and removing dampness. It is clinically used to treat lung carbuncle, lung heat cough and asthma, sore throat; dysentery, carbuncle, sore throat, snake and insect bites and other diseases. Scrophularia ningpoensis is cold in nature, sweet, bitter, and salty in taste. It belongs to the spleen, stomach, and kidney meridians. It has the functions of clearing heat and cooling blood, nourishing yin and reducing fire, detoxifying and dispersing knots. It is clinically used to treat many diseases such as febrile diseases with heat entering the blood, body heat and thirst, bone steaming and fatigue cough, sore throat, scrofula, sputum nucleus, carbuncle, sore throat, etc. Momordica grosvenori is cool in nature and sweet in taste. It belongs to the lung and large intestine meridians. It has the functions of clearing heat and moistening the lungs, relieving cough, relieving sore throat, and lubricating the intestines and relieving constipation. It is often used clinically to treat lung fire, dry cough, sore throat and aphonia.

[0017] Lycianthes biflora, also known as mountain blue, red blue, Guanyin grass, comes from the whole herb of Peristrophe roxburghiana (Schult.) Brem. of the Acanthaceae family. It is mainly produced in Yunnan, southern Sichuan, Guangxi, Guangdong, Jiangxi, Fujian and other places. As a folk herbal medicine, it is often used to relieve cough and expectorant, and treat hemoptysis, traumatic injury and other symptoms. The "Handbook of Commonly Used Chinese Herbal Medicines" states: "It is light and cool, dissipates blood stasis, stops bleeding, and treats contusions, acute sprains, and internal injuries and hemoptysis." The "National Compilation of Chinese Herbal Medicines" states: "It is sweet, light, and cool. It clears the lungs and stops coughs, dissipates blood stasis and stops bleeding. It treats hemoptysis due to tuberculosis, pneumonia, diabetes, and swelling and pain caused by traumatic injury."

[0018] "The liver is born on the left, and the lung is hidden on the right." The liver qi rises from the left, and the lung qi descends from the right. It is appropriate for the liver qi to rise, and it is smooth for the lung qi to descend. This is the characteristic of the rise and fall of the liver and lung qi. The liver rises and the lung descends, and the rise and fall are coordinated, which plays an important regulatory role in the regulation of the whole body's qi and the harmony of qi and blood. The ancients called it "the dragon and the tiger circle". Under pathological conditions, liver and lung diseases can affect each other. For example, if the liver is depressed and turns into fire, or the liver qi rises, and the liver fire rises, it can damage the lung yin, so that the lung qi cannot descend, and cough, chest pain, hemoptysis and other liver fire attack lung symptoms appear. The Yin-Yang theory calls it "the left rises too much, the right falls too little", and the Five Elements theory calls it "wood fire punishes gold" or "wood is strong and insults gold". On the other hand, if the lungs lose their clearing and clearing, and dryness and heat are excessive inside, it can also damage the liver yin, causing liver yang to be hyperactive, and headaches, irritability, flank pain and other lung and liver symptoms appear. The inventors found in clinical work that in the acute attack period of chronic obstructive pulmonary disease, symptoms such as cold and dampness, phlegm heat, phlegm dampness, and blood stasis can be seen, while in the chronic remission period, the function of various organs declines and there are symptoms such as phlegm and dampness. Phlegm and dampness, if not eliminated for a long time, often cause qi stagnation in the liver, liver depression turns into fire, and further damages the lung yin, resulting in liver fire attacking the lung, leading to acute attack of chronic obstructive pulmonary disease or long-term treatment. Therefore, while treating the lungs, more attention should be paid to clearing the liver and soothing the liver. In the present invention, the red silk thread has the effect of clearing the liver and reducing turbidity. In addition to clearing the liver and purging fire, it also removes phlegm in the middle jiao, which complements the monk fruit that clears the phlegm in the upper jiao.

[0019] The inventors found in previous experiments that the lung-clearing and cough-relieving effects of the water extract of red silk thread are significantly stronger than those of the alcohol extract of red silk thread, and the liver-clearing and turbidity-reducing effects of the alcohol extract of red silk thread are significantly stronger than those of the water extract of red silk thread. Therefore, the alcohol extract of red silk thread is used in the combination of the present invention.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention uses red silk thread, a Lingnan specialty medicinal material, as one of the raw materials, which produces a synergistic effect with Momordica grosvenori to improve the overall therapeutic effect of the Chinese medicine composition on COPD mice. Specifically, it can effectively reduce the degree of lung obstruction, improve the ability of lung expansion, and improve the tissue elasticity of the lung.

[0022] 2. The present invention adopts the common Lingnan specialty medicinal materials red silk thread and monk fruit, reduces the amount of precious Chinese medicinal materials Rhodiola rosea used in the prescription, and obtains better therapeutic effects. It enriches the selectivity of the drug base, reduces the degree of dependence on a single medicinal material, and improves the sustainable utilization and environmental friendliness of the medicinal material source.

[0023] 3. The present invention application can reduce the production cost of traditional Chinese medicine to a certain extent, increase the economic profit margin of enterprises, and have good economic and social benefits for society. DETAILED DESCRIPTION

[0024] Example 1

[0025] The red silk thread used in the experiment was purchased from the Chinese medicine wholesale market in Yulin, Guangxi. It was identified as the red silk thread of the genus Acanthaceae by Professor Tian Hui of Guangxi University of Chinese Medicine. The other medicinal materials were purchased from Sinopharm Holding Guangxi Co., Ltd., the same below; the specific dosage is shown in Table 1, and the specific steps are as follows:

[0026] (1) Weigh the red silk thread, crush it, add 75% ethanol at a mass ratio of 1g:7ml, heat and reflux at 80°C for 2h, filter the alcohol extract, recover the residue, add 75% ethanol at a mass ratio of 1g:5ml, continue to heat and reflux for 1.5h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract;

[0027] (2) respectively taking rhodiola rosea, astragalus, epimedium, red peony root, golden buckwheat, momordica grosvenori, Scrophularia ningpoensis, golden buckwheat, and liquorice, crushing and mixing, adding 15 times the weight of water, heating and extracting at 95°C for 60 minutes, filtering, and setting aside the filtrate; taking the filter residue, adding 10 times the weight of water, heating and extracting at 95°C for 30 minutes, filtering, combining the filtrates obtained twice, and concentrating to a thick paste;

[0028] (3) The red thread silk extract obtained in step (1) and the thick paste obtained in step (2) are stirred and mixed to obtain a product.

[0029] Example 2

[0030] The specific dosage is shown in Table 1. The specific steps are as follows:

[0031] (1) Weigh the red silk thread, crush it, add 75% ethanol at a mass ratio of 1g:7ml, heat and reflux at 80°C for 2h, filter the alcohol extract, recover the residue, add 75% ethanol at a mass ratio of 1g:5ml, continue to heat and reflux for 1.5h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract;

[0032] (2) respectively taking rhodiola rosea, astragalus, epimedium, red peony root, golden buckwheat, momordica grosvenori, Scrophularia ningpoensis, golden buckwheat, and liquorice, crushing and mixing, adding 10 times the weight of water, heating and extracting at 100°C for 45 minutes, filtering, and setting the filtrate aside; taking the filter residue, adding 8 times the weight of water, heating and extracting at 100°C for 20 minutes, filtering, combining the filtrates obtained twice, and concentrating to a thick paste;

[0033] (3) The red thread silk extract obtained in step (1) and the thick paste obtained in step (2) are stirred and mixed to obtain a product.

[0034] Example 3

[0035] The specific dosage is shown in Table 1. The specific steps are as follows:

[0036] (1) Weigh the red silk thread, crush it, add 75% ethanol at a mass ratio of 1g:7ml, heat and reflux at 80°C for 2h, filter the alcohol extract, recover the residue, add 75% ethanol at a mass ratio of 1g:5ml, continue to heat and reflux for 1.5h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract;

[0037] (2) respectively taking rhodiola rosea, astragalus, epimedium, red peony root, golden buckwheat, momordica grosvenori, Scrophularia ningpoensis, golden buckwheat, and liquorice, crushing and mixing, adding 10 times the weight of water, heating and extracting at 95°C for 60 minutes, filtering, and setting aside the filtrate; taking the filter residue, adding 8 times the weight of water, heating and extracting at 95°C for 30 minutes, filtering, combining the filtrates obtained twice, and concentrating to a thick paste;

[0038] (3) The red thread silk extract obtained in step (1) and the thick paste obtained in step (2) are stirred and mixed to obtain a product.

[0039] Comparative Example 1

[0040] The dosage is shown in Table 1. Unlike Example 3, no red thread is added. The other preparation methods are the same as Example 3.

[0041] Comparative Example 2

[0042] The dosage is shown in Table 1. Unlike Example 3, no Momordica grosvenori is added. The other preparation methods are the same as Example 3.

[0043] Comparative Example 3

[0044] The dosage is shown in Table 1. Unlike Example 3, Rhodiola rosea was not added. The other preparation methods were the same as Example 3.

[0045] Comparative Example 4

[0046] The dosage is shown in Table 1. Unlike Example 3, no monk fruit and red thread were added. The other preparation methods were the same as Example 3.

[0047] Comparative Example 5

[0048] The dosage is shown in Table 1. Different from Example 3, red silk thread and other medicinal materials are also extracted with water. The specific preparation method is: take Rhodiola rosea, Astragalus, Epimedium, Red Peony Root, Golden Buckwheat, Momordica grosvenori, Scrophularia, Golden Buckwheat, Licorice, and red silk thread respectively, crush and mix, add 10 times the weight of water, heat and extract at 95°C for 60 minutes, filter, and the filtrate is reserved; take the filter residue, add 8 times the weight of water, heat and extract at 95°C for 30 minutes, filter, combine the filtrates obtained twice, and concentrate to a thick paste.

[0049] Table 1

[0050] Material Type Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Rhodiola rosea 15 20 20 20 20 / 40 20 Astragalus 10 15 15 15 15 15 25 15 Epimedium 5 10 10 10 10 10 15 10 Red Peony Root 5 10 10 10 10 10 15 10 Golden buckwheat 5 10 10 10 10 10 15 10 Scrophularia 5 10 10 10 10 10 15 10 Scutellaria baicalensis 5 10 10 10 10 10 15 10 Licorice 10 15 15 15 15 15 15 15 Monk Fruit 20 25 25 65 / 35 / 25 Red thread 30 35 40 / 65 50 / 40

[0051] Experimental study on the effect of COPD on lung function in mice

[0052] 1. Experimental Materials

[0053] 1. Experimental animals: SPF-grade C57BL / 6J mice, male, 6-8 weeks old, weighing 20±2 g, provided by the Experimental Animal Center of Guilin Medical College (SYXK Gui 2020-0005), rearing environment: room temperature 24±2℃, relative humidity 55±5%, fed with standard feed, free access to food and water.

[0054] 2. Experimental reagents and instruments: sodium pentobarbital (Sigma-Aldrich, USA); Zhenlong brand cigarettes (Guangxi China Tobacco Industry Co., Ltd.); FlexiVent animal lung function measuring instrument (SCIREO, Canada).

[0055] 2. Modeling method

[0056] After one week of adaptive feeding, 100 SPF male C57BL / 6J mice were randomly divided into a blank group, a model group, Example 1 to 3 groups, and Comparative Example 1 to 4 groups, with 10 mice in each group. The COPD mouse model was established in the model group, Example 1 to 3 groups, and Comparative Example 1 to 6 groups of mice by the following method.

[0057] The specific process is as follows: LPS solution (30 μg / 6 μL) was dripped into the respiratory tract of mice from the nasal cavity on the 1st, 29th and 57th days, respectively, with a dosage of 1.5 mg / kg. Cigarette smoke exposure was continued from the 2nd to the 84th day (except the 29th and 57th days): the mice were placed in an animal transport box (60 cmx45 cmx20 cm) with ventilation windows, and 25 mice were sealed with tape in each box. The animal transport box was placed in a glass fumigation box, and a circular ventilation hole with a diameter of about 2 cm was left on each diagonal wall of the fumigation box to adjust the oxygen and air pressure inside and outside the box. Light a cigarette, insert it on a homemade metal burning rack, put it in a homemade glass fumigation box, cover it, and burn 10 cigarettes each time, 10 minutes / time, 2 times / day, with a 15-minute interval in between, and open the fumigation box cover for ventilation during the interval, 7 days / week, for a total of 12 weeks. After each cigarette smoke exposure, the animal transport box was placed in the transfer window, disinfected by ultraviolet light for 15 minutes, and then the mice were returned to the cage. The blank group was dripped with 0.9% sodium chloride solution (6L / mouse) from the nasal cavity to the respiratory tract on days 1, 29, and 57, and was kept normally for the rest of the time.

[0058] After modeling, the pathological manifestations of the mouse lung tissue were observed to be degeneration, necrosis, and shedding of the bronchial mucosal epithelium, shortened and adhered cilia; hyperplasia of goblet cells and mucus cells, a large amount of mucus retention, congestion and edema of the tracheal wall, and narrowing of the tracheal cavity. The alveoli atrophied and collapsed, the alveolar septa were destroyed and broken, and the adjacent alveoli fused into bullae. There was a large number of chronic inflammatory cell infiltration around the trachea and alveoli, the smooth muscle of the submucosal muscle layer was broken and atrophied, and the fibrous tissue proliferated; the bronchial structure was remodeled, the collagen content increased, and scar formation occurred, which was a successful modeling.

[0059] 3. Method of administration

[0060] According to the equivalent dose coefficient conversion method (the conversion coefficient of 60 kg human and 0.02 kg mouse is 9.1), the specific converted dosage is shown in Table 2. After the model is successfully established, each group of mice is given the corresponding dose of drugs by oral gavage according to Table 2. Before the drug is used, the thick paste mixture prepared by Examples 1 to 3 and Comparative Examples 1 to 4 is prepared with distilled water to the concentration required for the dosage volume. The blank group and the model group are given an equal volume of distilled water by gavage, twice a day, for 20 consecutive days.

[0061] Table 2 Dosage groups and mouse dosing design

[0062]

[0063] 4. Index detection

[0064] After the last administration, mice were anesthetized by intraperitoneal injection of 2% pentobarbital (0.1 mL / 10 g). The mice were fixed in a supine position on the operating table. The neck fur was cut with ophthalmic scissors to expose the subcutaneous tissue, and the trachea was bluntly separated. An incision was made in the horizontal direction of the distal end of the trachea, and the tracheal connector of the cannula was inserted into the trachea obliquely toward the heart. After fixing with cotton thread, the ventilator and the tracheal connector were connected. After conventional mechanical ventilation, respiratory function parameters such as respiratory system resistance (Rrs, the degree of lung obstruction), respiratory system elasticity (Ers, the elastic hardness of the lungs), and respiratory system compliance (Crs, the ability of the lungs to expand) were measured. The specific results are shown in Table 3.

[0065] Connect a 1ml syringe filled with sterile PBS to the endotracheal cannula and slowly push it for lavage. After injecting 0.8mL each time, slowly aspirate it. Repeat this three times and put the lavage fluid into a 1.5ml EP tube. Centrifuge at 3000rpm for 15min. Take the supernatant and store it at -80℃ for testing. Determine IL-6 and IL-13 according to the ELISA kit. The specific results are shown in Table 4.

[0066] 5. Statistical Analysis

[0067] The statistical software SPSS25.0 was used to analyze the experimental data and the normality test was performed on the measurement data. The indicators that conformed to the normal distribution were used. One-way ANOVA test was used for comparison among multiple groups, and LSD-t test was used for comparison between two groups. P < 0.05 indicated that the difference was statistically significant.

[0068] Table 3 Comparison of respiratory function parameters

[0069]

[0070] Note: Compared with the blank group, the model group had P*<0.05; compared with the model group, each treatment group had P#<0.05.

[0071] Table 4 Comparison of inflammatory factor levels in animal tissues

[0072] IL-6 (pg / ml) IL-13 (pg / ml) Blank Group 99.85±10.33 11.25±6.24 Model Group 521.30±24.52* 87.35±22.36* Example 1 287.68±17.45# 20.64±10.18# Example 2 206.29±15.38# 16.48±9.85# Comparative Example 1 419.86±23.84 65.21±28.25 Comparative Example 2 457.32±19.65 72.37±13.83

[0073] Note: Compared with the blank group, the model group had P*<0.05; compared with the model group, each treatment group had P#<0.05.

[0074] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A red thread traditional Chinese medicine composition for treating chronic obstructive pulmonary disease, wherein the red thread traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-20 parts of Rhodiola rosea, 10-15 parts of Astragalus, 5-10 parts of Epimedium, 5-10 parts of Red Peony Root, 5-10 parts of Scrophularia, 5-10 parts of Fagopyrum scabra, 5-10 parts of Scutellaria baicalensis, 10-15 parts of Licorice, 20-25 parts of Momordica grosvenori, and 30-40 parts of red thread.

2. The red thread traditional Chinese medicine composition according to claim 1, characterized in that The red thread traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of Rhodiola rosea, 10 parts of Astragalus, 10 parts of Epimedium, 10 parts of Red Peony Root, 10 parts of Scrophularia ningpoensis, 10 parts of Fagopyrum scabra, 10 parts of Scutellaria baicalensis, 15 parts of Licorice, 25 parts of Momordica grosvenori and 35 parts of red thread.

3. The red thread traditional Chinese medicine composition according to claim 1 or 2, characterized in that: The red thread is an ethanol extract of red thread.

4. The red thread traditional Chinese medicine composition according to claim 3, characterized in that The preparation method of the ethanol extract of the red silk thread is as follows: weigh the red silk thread, crush it, add ethanol with a mass concentration of 75% at a mass ratio of 1g:5-7ml, heat and reflux at 80°C for 1.5-2h, filter the ethanol extract, recover the residue, add ethanol with a mass concentration of 75% at a mass ratio of 1g:5-7ml, continue to heat and reflux for 1.5-2h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk thread extract.

5. A method for preparing the red thread silk Chinese medicine composition according to claim 3, characterized in that: The preparation method comprises the following steps: (1) Weigh the red silk thread, crush it, add 75% ethanol at a mass ratio of 1g:5-7ml, heat and reflux at 80°C for 1.5-2h, filter the alcohol extract, recover the residue, add 75% ethanol at a mass ratio of 1g:5-7ml, continue to heat and reflux for 1.5-2h. Combine the two ethanol extracts, concentrate and evaporate to dryness to prepare the red silk extract; (2) respectively taking rhodiola rosea, astragalus, epimedium, red peony root, golden buckwheat, monk fruit, Scrophularia ningpoensis, golden buckwheat, and liquorice, crushing and mixing, adding 10 to 15 times the weight of water, heating and extracting at 95 to 100° C. for 45 to 60 minutes, filtering, and setting the filtrate aside; taking the filter residue, adding 8 to 10 times the weight of water, heating and extracting at 95 to 100° C. for 20 to 30 minutes, filtering, combining the filtrates obtained twice, and concentrating to a thick paste; (3) The red thread silk extract obtained in step (1) and the thick paste obtained in step (2) are stirred and mixed to obtain a product.

6. Use of the red thread silk Chinese medicine composition described in any one of claims 1, 2, and 4 in the preparation of drugs for treating chronic obstructive pulmonary disease.

7. Use of the red thread traditional Chinese medicine composition described in claim 3 in preparing drugs for treating chronic obstructive pulmonary disease.

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