Filtration and purification process of tetraacetyl phytosphingosine fermentation lysate filtrate

Abstract

The present invention relates to the technical field of filtrate purification, and discloses a filtrate filtration and purification process of a tetraacetyl phytosphingosine fermentation lysate, including a mounting base, a stirring tank is provided on the mounting base, a driving motor is provided on the stirring tank, a stirring rod is provided at the output end of the driving motor, a fixing collar is provided on the stirring rod, a plurality of groups of deflection rods are rotatably provided on the fixing collar, a stirring blade is provided on the deflection rod, and an auxiliary mixing component is provided in the mounting base; a filter box is provided through one side of the stirring tank, a valve is provided at one end of the filter box, and a filter component is provided in the filter box; the auxiliary mixing component is used for auxiliary mixing of the filtrate of the stirring tank; the filter component is used to improve the convenience of replacing the filter screen when the mixed filtrate in the stirring tank is separated and filtered. Compared with the prior art, the present application effectively improves the purification efficiency of the tetraacetyl phytosphingosine fermentation lysate, and has good operability and economy, and is suitable for industrial application.

Description

Technical Field

[0001] The invention relates to the technical field of filtrate purification, in particular to a filtration and purification process of a filtrate of a tetraacetyl phytosphingosine fermentation lysate product. Background Art

[0002] The acetylation product of sphingosine is tetraacetyl phytosphingosine (TAPS). TAPS can stimulate the synthesis of glucosylceramide in vitro. Glucosylceramide has a protective function, helps to strengthen the skin barrier function, inhibit inflammation, inhibit angiogenesis, and lighten dark circles. Tetraacetyl phytosphingosine has good solubility and can be compounded with a variety of cosmetic raw materials. It is suitable for anti-aging, repair and eye cream products; tetraacetyl phytosphingosine is a biochemical product with broad application prospects, commonly used in medicine, cosmetics and agriculture.

[0003] The production process of tetraacetyl phytosphingosine is usually obtained by fermentation, but during the fermentation process, the lysate will contain a large amount of impurities and insolubles, affecting the quality of the final product. At present, for the purification process of fermentation lysate, traditional separation and purification methods such as sedimentation, centrifugation and conventional filtration can remove impurities to a certain extent, but often have problems such as complex operation, low efficiency, high energy consumption, and frequent filter replacement; Therefore, the present application discloses a filtration and purification process for the filtrate of tetraacetyl phytosphingosine fermentation lysate to meet the needs of extracting and purifying tetraacetyl phytosphingosine from fermentation lysate. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the shortcomings of the prior art, the present invention provides a filtration and purification process for the filtrate of a tetraacetyl phytosphingosine fermentation lysate, which has the advantages of effectively improving the purification efficiency of the tetraacetyl phytosphingosine fermentation lysate, and solves a series of problems in the traditional separation process, such as complex operation, low efficiency, high energy consumption, and frequent filter replacement.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a filtration and purification process of a filtrate of a tetraacetyl phytosphingosine fermentation lysate, comprising a mounting base, a stirring tank is arranged on the mounting base, a driving motor is arranged on the stirring tank, a stirring rod is arranged at the output end of the driving motor, a fixing collar is sleeved on the stirring rod, and an auxiliary mixing component is arranged in the mounting base; a filter box is arranged through one side of the stirring tank, a valve is arranged at one end of the filter box, and a filter component is arranged in the filter box; the auxiliary mixing component is used for auxiliary mixing of the filtrate of the stirring tank; the filter component is used for improving the convenience of replacing the filter when separating and filtering the mixed filtrate in the stirring tank.

[0008] Preferably, the auxiliary mixing assembly includes a mounting seat arranged at the bottom of the stirring tank, a mounting plate is arranged on the mounting seat, a mounting guard plate is arranged on the mounting plate, the mounting plate is located inside the mounting guard plate, a driving gear ring is rotatably arranged on the mounting plate, a connecting hole is opened at the center of the driving gear ring, a plurality of guide grooves are opened on the inner wall of the connecting hole, a group of movable gear plates are meshed on the driving gear ring, a group of movable gear plates are each provided with a synchronization rod at one end, a through groove is opened on the mounting guard plate, the synchronization rod passes through the through groove, a guide rod is arranged at one end of the synchronization rod, a contact arc plate is arranged at one end of the guide rod, a reset spring is sleeved on the guide rod, one end of the reset spring is arranged on one side of the mounting guard plate, and the other end of the reset spring is arranged on one side of the contact arc plate, and the contact arc plate is in the shape of an arc bent to one side.

[0009] Preferably, the auxiliary mixing assembly also includes a threaded rod arranged at the bottom of the stirring rod, the threaded rod is provided with a reciprocating double thread, limit rings are provided at both ends of the threaded rod, a movable sleeve is provided on the threaded sleeve of the threaded rod, and multiple groups of extension plates are provided on the movable sleeve, and a first driving rod is provided on the surface of the extension plate, and the first driving rod is in contact with the guide groove.

[0010] 4. The filtration of tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 1 is characterized in that: the filtration assembly includes a group of filter plates slidably arranged in the filter box, a fixed plate is arranged on the filter box, a second driving rod is rotatably arranged in the fixed plate, the second driving rod passes through the filter box, a rotating disk is arranged at one end of the second driving rod, a first rotating rod and a second rotating rod are also rotatably arranged in the fixed plate, the second rotating rod and the second rotating rod are rotatably arranged at both ends with synchronous connecting rods, the synchronous connecting rod is rotatably connected with the second driving rod, a deflection arm is arranged on the first rotating rod and the second rotating rod, a rotating seat is rotatably arranged at one end of the deflection arm, and an extension arm is rotatably arranged in the rotating seat.

[0011] Preferably, a group of support seats are provided on one side of the bottom of the mounting base, a flip plate is rotatably provided on the support seat, the flip plate is fixed to the bottom of the mixing tank, a group of fixed sockets are provided at the bottom of the mixing tank, and bolts are threaded on the fixed sockets.

[0012] Preferably, a plurality of groups of deflection rods are rotatably arranged on the fixed collar, and stirring blades are arranged on the deflection rods.

[0013] Preferably, a group of guide plates are arranged on the mounting plate, the guide plates are arranged at the bottom of the movable tooth plate, the guide plates are arranged at an angle, and one end of the movable tooth plate is adapted to the inclination angle of the guide plates.

[0014] Preferably, a plurality of mounting grooves are provided on one side surface of the contact arc plate, a connecting rod is provided in the mounting groove, a deflection plate is provided on the connecting rod, a mixing plate is provided at one end of the deflection plate, the mixing plate and the deflection plate are arranged in an I-shape, a contact plate is provided at the other end of the deflection plate, a limiting plate is provided on the mounting groove, and the contact plate is in contact with the limiting plate.

[0015] Preferably, a limit block is provided at the bottom of the filter plate, a guide rod is provided at one end of the limit block, the guide rod is a retractable structure, a second contact head is provided at one end of the guide rod, a buffer spring is sleeved on the guide rod, one end of the buffer spring is provided on one side of the limit block, the other end of the buffer spring is provided on one side of the second contact head, a first contact head is provided at one end of the extension arm, the first contact head is arranged in a trapezoidal shape, and the first contact head is adapted to the second contact head.

[0016] The process for purifying the filtrate of the tetraacetyl phytosphingosine fermentation lysate is applied to the filtration of the filtrate of the tetraacetyl phytosphingosine fermentation lysate described in any one of the above items, comprising the following steps:

[0017] S1: Preparation of fermentation lysate filtrate, fermentation of tetraacetyl phytosphingosine, discharge of fermentation broth from the fermentation tank, and preparation for the next step of mixing and filtration;

[0018] S2: stirring and mixing, adding auxiliary mixing components to the stirring tank, driving the stirring rod through the driving motor, and using the threaded rod 9 and the moving collar 10 to improve the mixing efficiency;

[0019] S3: Filtration, coarse filtration of the filtrate through the filter box to remove larger particles, using a sliding filter plate to ensure easy replacement of the filter screen; regulating the flow of the filtrate through the valve;

[0020] S4: Solution treatment and collection, the refined tetraacetyl phytosphingosine solution, and the final purified solution can be further concentrated and dried as needed to finally obtain the target product.

[0021] (III) Beneficial effects

[0022] Compared with the prior art, the present invention provides a filtration and purification process for the filtrate of the tetraacetyl phytosphingosine fermentation lysate, which has the following beneficial effects:

[0023] 1. The filtration and purification process of the filtrate of the fermentation lysate of tetraacetyl phytosphingosine. The fermentation lysate filtrate in the stirring tank is fully stirred by driving a stirring rod driven by a driving motor to ensure that the filtrate is evenly mixed. The auxiliary mixing component arranged in the stirring tank further helps to mix the filtrate, enhances the solubility and the uniformity of the distribution of particles. Then, the filtrate enters through the filter box on one side of the stirring tank, and is separated and filtered by the filter component arranged in the filter box. The filter box is equipped with a valve, which can conveniently control the flow of the filtrate. At the same time, the filter component makes the filter screen replacement more convenient, further improving the work efficiency and the convenience of operation; the auxiliary mixing component is arranged, so that the filtrate can be fully mixed during the filtration process, and the filtration efficiency is improved. At the same time, the filter component and the filter screen replacement method greatly simplify the filter screen replacement operation, reduce the cumbersome steps of manual operation, save time and cost, and this process can effectively improve the purification efficiency of the tetraacetyl phytosphingosine fermentation lysate, and has good operability and economy, and is suitable for industrial application.

[0024] 2. The filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate product is started by a driving motor to drive the stirring rod to rotate. When the stirring rod rotates, the threaded rod can be synchronously driven to rotate. When the threaded rod rotates, the reciprocating double threads provided on the threaded rod and the first driving rod contact the guide groove at the same time, so that the movable sleeve ring can move along the axis of the threaded rod, so that the first driving rod moves along the axis of the connecting hole under the guidance of the guide groove. When the first driving rod moves under the guidance of the guide groove, it can drive the driving gear ring to rotate, and a group of moving gear plates are engaged with the driving gear ring. At this time, the group of moving gear plates can be simultaneously moved in the opposite direction under the drive of the driving gear ring. At the same time, the guide rod can be driven to move through the synchronous rod, thereby driving the contact arc plate to move, and then the filtrate liquid in the stirring tank can be fully mixed and stirred. At the same time, when the movable sleeve moves to the bottom of the threaded rod, it can be raised through the reciprocating double thread structure on the threaded rod. At this time, the driving gear ring can be driven to rotate in the opposite direction by the first driving rod. At the same time, the elastic force of the reset spring can assist the driving gear ring to reset and rotate under the guidance of the guide rod, thereby completing the effect of periodic stirring of the liquid in the stirring tank, ensuring efficient and uniform mixing effect during the stirring process, effectively improving the mixing efficiency of the filtrate in the stirring tank, avoiding the aggregation of sediment or particulate matter, and ensuring the uniformity of the filtrate.

[0025] 3. In the filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate, when the movable tooth plate moves under the drive of the driving tooth ring, the movable tooth plate can contact the guide plate, so that the movable tooth plate moves along the path of the guide plate, so that the height of the synchronization rod changes through the groove, thereby achieving sufficient mixing of the liquid in the stirring tank during stirring, ensuring the coordinated longitudinal and lateral movements of the entire stirring system, and enhancing the mixing effect.

[0026] 4. The filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate, after the filtrate liquid in the stirring tank is stirred, the valve is opened to allow the liquid in the stirring tank to pass through the filter box and then flow out of the valve. In this process, the rotating disk is rotated to drive the second driving rod to rotate, and the synchronous connecting rod is rotated at this time, which can drive the first rotating rod and the second rotating rod to rotate synchronously, so as to achieve synchronous driving of the deflection arms on both sides of the fixed plate to rotate in the opposite direction. At the same time, the rotating seat can make the extension arm change in height while maintaining a stable state. When the extension arm is in the process of lifting, that is, when the extension arm moves from the lowest point to the highest point, the first contact head can be in contact with the second contact head. The filter plate can be lifted up synchronously with the extension arm when it is lifted, and the distance between the extension arm and the limit block can become smaller during the lifting of the extension arm. The elastic force of the buffer spring can prevent the extension arm from pressing the limit block and damaging the filter plate under the guidance of the guide rod. When the filter plate is lifted to the highest point, only the filter screen on the surface of the filter plate needs to be replaced to complete the replacement of the filter screen. At this time, the filter box can still filter the filtrate from the mixing tank, thereby achieving the effect of replacing the filter screen during the filtration process. It not only improves the convenience of replacing the filter screen, but also ensures the continuity of filtration of the filtrate, thereby effectively improving the filtration effect.

[0027] 5. In the filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate, after the filter screen on the filter plate surface is replaced, the filter plate can be reset by simply rotating the disc in the opposite direction. At this time, there are two filter plates in the filter box that filter the filtrate in the stirring tank at the same time. Compared with the traditional single filtration, the filtration effect is better, which not only improves the filtration efficiency and reduces the difficulty of operation, but also reduces the equipment maintenance cost, and has high economy and reliability.

[0028] 6. The filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate can easily tilt or flip the stirring tank through the rotating structure of the flip plate and the support seat, which is convenient for cleaning, maintenance or replacement of internal parts. At the same time, the fixed base and bolts ensure the stability and reliability of the stirring tank, avoid vibration or displacement during use, ensure the stability of the equipment during operation, not only improve the flexibility of operation, but also enhance the safety and durability of the equipment, which helps to improve the overall production efficiency and the service life of the equipment.

[0029] The tetraacetyl phytosphingosine fermentation lysate filtrate filtration and purification process effectively improves the mixing efficiency through the cooperation of the deflection plate and the mixing plate in an I-shaped layout, so that the liquid can be more evenly distributed during the stirring process. The cooperation of the contact plate and the limit plate ensures that the movement range of the mixing plate is controlled within a reasonable range, which not only improves the stirring effect, but also ensures the safety and durability of the equipment. It has good adaptability and adjustability, and effectively improves the degree of stirring and mixing of the filtrate.

[0030] , the filtration and purification process of the filtrate of the tetraacetyl phytosphingosine fermentation lysate effectively improves the purification efficiency of the solution through reasonable stirring, mixing and filtration design. First, the auxiliary mixing component in the stirring tank ensures the uniform stirring of the filtrate, so that the fermentation lysate is fully mixed before filtration, avoiding the precipitation of particulate matter. Then, the filter box and the filter component not only achieve coarse filtration to remove larger particulate impurities, but also improve the purity of the solution through fine filtration, ensuring the high-quality output of the target product. The convenience and adjustability of the filter replacement in the whole process greatly simplifies the operating steps, reduces maintenance time, and increases the service life of the equipment. In addition, by fixing the base and bolts, the stability and safety of the filtration process are guaranteed, and the risk of equipment failure is reduced. The process has high operating efficiency, stability and economy, and is suitable for the industrial production of tetraacetyl phytosphingosine. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0032] Figure 2 It is a schematic diagram of a partially cutaway three-dimensional structure of a stirring tank of the present invention;

[0033] Figure 3 It is a schematic diagram of the three-dimensional structure of the fixing seat of the present invention;

[0034] Figure 4 This is a schematic diagram of the three-dimensional structure of the stirring blade of the present invention;

[0035] Figure 5 It is a partially cutaway three-dimensional structural schematic diagram of the filter box of the present invention;

[0036] Figure 6 This is a schematic diagram of the structure of the vertical filter plate of the present invention;

[0037] Figure 7 It is a schematic diagram of the three-dimensional structure of the driving rod of the present invention;

[0038] Figure 8 It is a schematic diagram of the three-dimensional structure of the synchronous connecting rod of the present invention;

[0039] Fig. 9 For the present invention Figure 6A in the middle is an enlarged schematic diagram of the three-dimensional structure;

[0040] Fig.10 This is a schematic diagram of the internal three-dimensional structure of the stirring tank of the present invention;

[0041] Fig.11 This is a schematic diagram of the three-dimensional structure of the auxiliary mixing component of the present invention;

[0042] Fig.12 For the present invention Fig.11 The enlarged three-dimensional structure diagram at B in the middle;

[0043] Fig.13 It is a schematic diagram of the three-dimensional structure of the contact plate of the present invention;

[0044] Fig.14 It is a schematic diagram of a partially cutaway three-dimensional structure of a contact plate of the present invention.

[0045] In the figure: 1, mounting base; 2, stirring tank; 3, driving motor; 4, stirring rod; 5, fixed collar; 6, deflection rod; 7, stirring blade; 8, limiting ring; 9, threaded rod; 10, movable collar; 11, extension plate; 12, first driving rod; 13, filter box; 14, valve; 15, support seat; 16, flip plate; 17, fixed plate; 18, second driving rod; 19, rotating disc; 20, first rotating rod; 21, second rotating rod; 22, synchronous connecting rod; 23, deflection arm; 24, rotating seat; 25, extension arm; 26 , first contact head; 27, filter plate; 28, limit block; 29, guide rod; 30, buffer spring; 31, second contact head; 32, mounting seat; 33, mounting guard plate; 34, mounting plate; 35, through slot; 36, driving gear ring; 37, connecting hole; 38, guide slot; 39, moving gear plate; 40, synchronization rod; 41, guide rod; 42, reset spring; 43, guide plate; 44, contact arc plate; 45, connecting rod; 46, deflection plate; 47, mixing plate; 48, contact plate; 49, limit plate; 50, fixed base. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] As introduced in the background technology, the existing technology has deficiencies. In order to solve the above technical problems, the present application proposes a filtration and purification process for the filtrate of the fermentation lysate of tetraacetyl phytosphingosine.

[0048] In a typical implementation of the present application, Figure 1-Figure 14 As shown, the filtration and purification process of the filtrate of the fermentation lysate of tetraacetyl phytosphingosine comprises a mounting base 1, a stirring tank 2 is arranged on the mounting base 1, a driving motor 3 is arranged on the stirring tank 2, a stirring rod 4 is arranged at the output end of the driving motor 3, a fixing collar 5 is sleeved on the stirring rod 4, a plurality of groups of deflection rods 6 are rotatably arranged on the fixing collar 5, a stirring blade 7 is arranged on the deflection rod 6, an auxiliary mixing component is arranged in the mounting base 1; a filter box 13 is arranged through one side of the stirring tank 2, a valve 14 is arranged at one end of the filter box 13, and a filter component is arranged in the filter box 13; the auxiliary mixing component is used for auxiliary mixing of the filtrate of the stirring tank 2; the filter component is used for improving the convenience of replacing the filter screen when separating and filtering the mixed filtrate in the stirring tank 2;

[0049] First, the fermentation lysate filtrate in the stirring tank 2 is fully stirred by driving the stirring rod 4 through the driving motor 3 to ensure that the filtrate is evenly mixed. The auxiliary mixing component arranged in the stirring tank 2 further helps to mix the filtrate and enhance the solubility and uniformity of particle distribution. Then, the filtrate enters through the filter box 13 on one side of the stirring tank 2 and is separated and filtered by the filter component arranged in the filter box 13. The filter box 13 is equipped with a valve 14, which can conveniently control the flow of the filtrate. At the same time, the filter component makes the filter screen replacement more convenient, further improving the work efficiency and the convenience of operation; the auxiliary mixing component is arranged, so that the filtrate can be fully mixed during the filtration process, thereby improving the filtration efficiency. At the same time, the filter component and the filter screen replacement method greatly simplify the filter screen replacement operation, reduce the cumbersome steps of manual operation, save time and cost, and this process can effectively improve the purification efficiency of tetraacetyl phytosphingosine fermentation lysate, and has good operability and economy, and is suitable for industrial application.

[0050] As a preferred implementation in this embodiment, refer to the attached Figure 2 , Figure 4 , Figure 10-Figure 14The auxiliary mixing assembly includes a mounting seat 32 arranged at the bottom of the mixing tank 2, a mounting plate 34 is arranged on the mounting seat 32, a mounting guard plate 33 is arranged on the mounting plate 34, the mounting plate 34 is located inside the mounting guard plate 33, a driving gear ring 36 is rotatably arranged on the mounting plate 34, a connecting hole 37 is opened at the center of the driving gear ring 36, a plurality of guide grooves 38 are opened on the inner wall of the connecting hole 37, a group of moving tooth plates 39 are meshed on the driving gear ring 36, one end of each group of moving tooth plates 39 is provided with a synchronization rod 40, a through groove 35 is opened on the mounting guard plate 33, the synchronization rod 40 passes through the through groove 35, a guide rod 41 is arranged at one end of the synchronization rod 40, a contact arc plate 44 is arranged at one end of the guide rod 41, a reset spring 42 is sleeved on the guide rod 41, and the reset spring One end of the spring 42 is arranged on one side of the mounting guard plate 33, and the other end of the return spring 42 is arranged on one side of the contact arc plate 44, and the contact arc plate 44 is in an arc shape bent to one side; the auxiliary mixing assembly also includes a threaded rod 9 arranged at the bottom of the stirring rod 4, and a reciprocating double thread is provided on the threaded rod 9. Limiting rings 8 are arranged at both ends of the threaded rod 9, and a movable sleeve 10 is threadedly sleeved on the threaded rod 9. A plurality of groups of extension plates 11 are arranged on the movable sleeve 10, and a first driving rod 12 is arranged on the surface of the extension plate 11, and the first driving rod 12 is in contact with the guide groove 38; a group of guide plates 43 are arranged on the mounting plate 34, and the guide plates 43 are arranged at the bottom of the movable tooth plate 39, and the guide plates 43 are arranged in an inclined manner, and one end of the movable tooth plate 39 is adapted to the inclination angle of the guide plate 43;

[0051] By starting the driving motor 3, the stirring rod 4 is driven to rotate. When the stirring rod 4 rotates, the threaded rod 9 can be driven to rotate synchronously. When the threaded rod 9 rotates, the reciprocating double threads on the threaded rod 9 and the first driving rod 12 contact the guide groove 38 at the same time, so that the movable sleeve 10 can move along the axis of the threaded rod 9, so that the first driving rod 12 moves along the axis of the connecting hole 37 under the guidance of the guide groove 38. When the first driving rod 12 moves under the guidance of the guide groove 38, the driving gear ring 36 can be driven to rotate. A group of moving tooth plates 39 are engaged with the driving gear ring 36. At this time, a group of moving tooth plates 39 can be driven to move in the opposite direction under the drive of the driving gear ring 36 at the same time, and can be driven by the same The step rod 40 drives the guide rod 41 to move, thereby driving the contact arc plate 44 to move, and then the filtrate liquid in the stirring tank 2 can be fully mixed and stirred. At the same time, when the movable sleeve ring 10 moves to the bottom of the threaded rod 9, it can be lifted up through the reciprocating double thread structure on the threaded rod 9. At this time, the driving gear ring 36 can be driven to rotate in the opposite direction through the first driving rod 12. At the same time, the elastic force of the reset spring 42 can assist the driving gear ring 36 to reset and rotate under the guidance of the guide rod 41, thereby completing the periodic stirring of the liquid in the stirring tank 2, ensuring an efficient and uniform mixing effect during the stirring process, and effectively improving the mixing efficiency of the filtrate in the stirring tank 2, avoiding the aggregation of sediments or particles, and ensuring the uniformity of the filtrate;

[0052] At the same time, when the movable tooth plate 39 moves under the drive of the driving gear ring 36, the movable tooth plate 39 can contact the guide plate 43, so that the movable tooth plate 39 moves along the path of the guide plate 43, so that the synchronization rod 40 changes its height through the groove 35, thereby achieving sufficient mixing of the liquid in the stirring tank 2 during stirring, ensuring the coordinated longitudinal and lateral movements of the entire stirring system, and enhancing the mixing effect.

[0053] As a preferred implementation in this embodiment, refer to the attached Figure 5-Figure 9The filter assembly includes a group of filter plates 27 slidably arranged in the filter box 13, a fixed plate 17 is arranged on the filter box 13, a second drive rod 18 is rotatably arranged in the fixed plate 17, the second drive rod 18 passes through the filter box 13, a rotating disc 19 is arranged at one end of the second drive rod 18, a first rotating rod 20 and a second rotating rod 21 are also rotatably arranged in the fixed plate 17, the second rotating rod 21 and the two ends of the second rotating rod 21 are rotatably arranged with a synchronous connecting rod 22, the synchronous connecting rod 22 is rotatably connected to the second drive rod 18, a deflection arm 23 is arranged on the first rotating rod 20 and the second rotating rod 21, and one end of the deflection arm 23 is rotatably arranged A rotating seat 24 is rotatably provided at the end, and an extension arm 25 is rotatably provided in the rotating seat 24; a limit block 28 is provided at the bottom of the filter plate 27, and a guide rod 29 is provided at one end of the limit block 28. The guide rod 29 is a telescopic structure, and a second contact head 31 is provided at one end of the guide rod 29. A buffer spring 30 is sleeved on the guide rod 29, and one end of the buffer spring 30 is arranged on one side of the limit block 28, and the other end of the buffer spring 30 is arranged on one side of the second contact head 31. A first contact head 26 is provided at one end of the extension arm 25, and the first contact head 26 is arranged in a trapezoidal shape, and the first contact head 26 is adapted to the second contact head 31;

[0054] When the filtrate liquid in the mixing tank 2 is stirred, the valve 14 is opened to allow the liquid in the mixing tank 2 to pass through the filter box 13 and then flow out of the valve 14. In this process, the rotating disk 19 is rotated to drive the second driving rod 18 to rotate, and the synchronous connecting rod 22 is rotated, which can drive the first rotating rod 20 and the second rotating rod 21 to rotate synchronously, so as to synchronously drive the deflection arms 23 on both sides of the fixed plate 17 to rotate in the opposite direction. At the same time, through the rotating seat 24, the extension arm 25 can change its height while maintaining a stable state. When the extension arm 25 is in the process of lifting and lowering, that is, when the extension arm 25 moves from the lowest point to the highest point, it can engage with the second contact head 31 through the first contact head 26, thereby driving the limit block 28 The filter plate 27 can be raised synchronously with the extension arm 25. At this time, during the raising process of the extension arm 25, the distance between the extension arm 25 and the limit block 28 will become smaller. Under the guidance of the guide rod 29, the elastic force of the buffer spring 30 can make the extension arm 25 drive the limit block 28 to rise, thereby preventing the extension arm 25 from causing pressure on the limit block 28, thereby causing damage to the filter plate 27. When the filter plate 27 is lifted to the highest point, it is only necessary to replace the filter screen on the surface of the filter plate 27 to complete the replacement of the filter screen. At this time, the filter box 13 can still filter the filtrate of the mixing tank 2, thereby achieving the effect of replacing the filter screen during the filtration process, which not only improves the convenience of replacing the filter screen, but also ensures the continuity of filtration of the filtrate, and effectively improves the filtration effect.

[0055] When the filter screen on the surface of the filter plate 27 is replaced, the filter plate 27 can be reset by simply rotating the disc 19 in the opposite direction. At this time, there are two filter plates 27 in the filter box 13 that filter the filtrate in the mixing tank 2 at the same time. Compared with the traditional single filtration, the filtration effect is better, which not only improves the filtration efficiency and reduces the operation difficulty, but also reduces the equipment maintenance cost, and has higher economy and reliability.

[0056] As a preferred implementation in this embodiment, refer to the attached Figure 1-Figure 3 , Figure 5 A group of support seats 15 are arranged on one side of the bottom of the mounting base 1, and a flip plate 16 is rotatably arranged on the support seat 15. The flip plate 16 is fixedly arranged with the bottom of the mixing tank 2. A group of fixed clamping seats 50 are arranged at the bottom of the mixing tank 2, and bolts are threadedly arranged on the fixed clamping seats 50;

[0057] Through the rotating structure of the flip plate 16 and the support seat 15, the mixing tank 2 can be easily tilted or flipped, which is convenient for cleaning, maintenance or replacement of internal components. At the same time, the fixed base 50 and the bolts ensure the stability and reliability of the mixing tank 2, avoid vibration or displacement during use, and ensure the stability of the equipment during operation, which not only improves the flexibility of operation, but also enhances the safety and durability of the equipment, and helps to improve the overall production efficiency and the service life of the equipment.

[0058] As a preferred implementation in this embodiment, refer to the attached Figure 10-Figure 14 A plurality of mounting grooves are provided on one side surface of the contact arc plate 44, a connecting rod 45 is provided in the mounting groove, a deflection plate 46 is provided on the connecting rod 45, a mixing plate 47 is provided at one end of the deflection plate 46, the mixing plate 47 and the deflection plate 46 are arranged in an I-shape, a contact plate 48 is provided at the other end of the deflection plate 46, a limiting plate 49 is provided on the mounting groove, and the contact plate 48 contacts the limiting plate 49;

[0059] The cooperation between the deflection plate 46 and the mixing plate 47 in an I-shaped layout effectively improves the mixing efficiency, so that the liquid can be more evenly distributed during the stirring process. The cooperation between the contact plate 48 and the limit plate 49 ensures that the movement range of the mixing plate 47 is controlled within a reasonable range, which not only improves the stirring effect, but also ensures the safety and durability of the equipment. It has good adaptability and adjustability, and effectively improves the degree of stirring and mixing of the filtrate.

[0060] The process for purifying the filtrate of the tetraacetyl phytosphingosine fermentation lysate is applied to the filtration of the filtrate of the tetraacetyl phytosphingosine fermentation lysate described in any one of the above items, comprising the following steps:

[0061] S1: Preparation of fermentation lysate filtrate, fermentation of tetraacetyl phytosphingosine, discharge of fermentation broth from the fermentation tank, and preparation for the next step of mixing and filtration;

[0062] S2: stirring and mixing, adding an auxiliary mixing component to the stirring tank 2, driving the stirring rod 4 through the driving motor 3, using the threaded rod 9 and the moving collar 10 to improve the mixing efficiency and ensure the uniformity of the lysed filtrate. The mixing in the stirring tank 2 ensures the uniformity of the liquid components and enhances the effect of subsequent filtration;

[0063] S3: Filtration: coarse filtration of the filtrate through the filter box 13 to remove larger particles, and use the sliding filter plate 27 to ensure convenient replacement of the filter screen; fine filtration of the lysate to remove smaller particles to ensure high purity of the filtered liquid, and the flow of the filtrate is adjusted by the valve 14;

[0064] S4: Solution treatment and collection, the refined tetraacetyl phytosphingosine solution, and the final purified solution can be further concentrated, dried or otherwise treated as needed to finally obtain a purified tetraacetyl phytosphingosine product.

[0065] Through reasonable stirring, mixing and filtering design, the purification efficiency of the solution is effectively improved. First, the auxiliary mixing component in the stirring tank 2 ensures the uniform stirring of the filtrate, so that the fermentation lysate is fully mixed before filtration, avoiding the precipitation of particulate matter. Then, the filter box 13 and the filter component not only realize the coarse filtration to remove larger particle impurities, but also improve the purity of the solution through fine filtration, ensuring the high-quality output of the target product. The convenience and adjustability of the filter replacement in the whole process greatly simplifies the operation steps, reduces maintenance time, and increases the service life of the equipment. In addition, by fixing the base 50 and the bolts, the stability and safety of the filtration process are guaranteed, and the risk of equipment failure is reduced. The process has high operating efficiency, stability and economy, and is suitable for the industrial production of tetraacetyl phytosphingosine.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for filtering a filtrate of a tetraacetyl phytosphingosine fermentation lysate, comprising: a mounting base (1), wherein a stirring tank (2) is arranged on the mounting base (1), and wherein: The stirring tank (2) is provided with a driving motor (3), the output end of the driving motor (3) is provided with a stirring rod (4), the stirring rod (4) is sleeved with a fixing collar (5), and an auxiliary mixing component is provided in the mounting base (1); a filter box (13) is provided through one side of the stirring tank (2), a valve (14) is provided at one end of the filter box (13), and a filter component is provided in the filter box (13); The auxiliary mixing component is used to assist in mixing the filtrate in the stirring tank (2); The filtering assembly is used to improve the convenience of replacing the filter screen when separating and filtering the mixed filtrate in the stirring tank (2).

2. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 1, characterized in that: The auxiliary mixing assembly comprises a mounting seat (32) arranged at the bottom of the stirring tank (2), a mounting plate (34) being arranged on the mounting seat (32), a mounting guard plate (33) being arranged on the mounting plate (34), the mounting plate (34) being located inside the mounting guard plate (33), a driving gear ring (36) being rotatably arranged on the mounting plate (34), a connecting hole (37) being provided at the center of the driving gear ring (36), a plurality of guide grooves (38) being provided on the inner wall of the connecting hole (37), a group of movable tooth plates (39) being meshed on the driving gear ring (36), one of the movable tooth plates (39) being provided with a plurality of guide grooves (38) being provided on the inner wall of the connecting hole (37), A synchronization rod (40) is provided at each end, a through slot (35) is provided on the installation guard plate (33), and the synchronization rod (40) passes through the through slot (35), a guide rod (41) is provided at one end of the synchronization rod (40), and a contact arc plate (44) is provided at one end of the guide rod (41), and a return spring (42) is sleeved on the guide rod (41), one end of the return spring (42) is provided on one side of the installation guard plate (33), and the other end of the return spring (42) is provided on one side of the contact arc plate (44), and the contact arc plate (44) is in the shape of an arc bent toward one side.

3. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 2, characterized in that: The auxiliary mixing assembly also includes a threaded rod (9) arranged at the bottom of the stirring rod (4), the threaded rod (9) being provided with a reciprocating double thread, limit rings (8) being provided at both ends of the threaded rod (9), a movable sleeve (10) being provided on the threaded rod (9), a plurality of groups of extension plates (11) being provided on the movable sleeve (10), a first driving rod (12) being provided on the surface of the extension plate (11), and the first driving rod (12) being in contact with the guide groove (38).

4. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 1, characterized in that: The filter assembly comprises a group of filter plates (27) slidably arranged in the filter box (13); a fixed plate (17) is arranged on the filter box (13); a second driving rod (18) is rotatably arranged in the fixed plate (17); the second driving rod (18) passes through the filter box (13); a rotating disk (19) is arranged at one end of the second driving rod (18); a first rotating rod (20) and a second rotating rod (21) are also rotatably arranged in the fixed plate (17); a synchronous connecting rod (22) is rotatably arranged at both ends of the second rotating rod (21) and the second rotating rod (21); the synchronous connecting rod (22) is rotatably connected to the second driving rod (18); a deflection arm (23) is arranged on each of the first rotating rod (20) and the second rotating rod (21); a rotating seat (24) is rotatably arranged at one end of the deflection arm (23); an extension arm (25) is rotatably arranged in the rotating seat (24).

5. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 1, characterized in that: A group of support seats (15) are arranged on one side of the bottom of the mounting base (1), a flip plate (16) is rotatably arranged on the support seat (15), the flip plate (16) is fixedly arranged on the bottom of the stirring tank (2), a group of fixed clamping seats (50) are arranged at the bottom of the stirring tank (2), and bolts are threadedly arranged on the fixed clamping seats (50).

6. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 1, characterized in that: A plurality of groups of deflection rods (6) are rotatably arranged on the fixed collar (5), and stirring blades (7) are arranged on the deflection rods (6).

7. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 2, characterized in that: A group of guide plates (43) are arranged on the mounting plate (34), and the guide plates (43) are arranged at the bottom of the movable tooth plate (39). The guide plates (43) are arranged in an inclined manner, and one end of the movable tooth plate (39) is adapted to the inclination angle of the guide plates (43).

8. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 7, characterized in that: A plurality of mounting grooves are provided on one side surface of the contact arc plate (44), a connecting rod (45) is provided in the mounting groove, a deflection plate (46) is provided on the connecting rod (45), a mixing plate (47) is provided at one end of the deflection plate (46), the mixing plate (47) and the deflection plate (46) are arranged in an I-shape, a contact plate (48) is provided at the other end of the deflection plate (46), a limiting plate (49) is provided on the mounting groove, and the contact plate (48) is in contact with the limiting plate (49).

9. The filtration of the tetraacetyl phytosphingosine fermentation lysate filtrate according to claim 4, characterized in that: A limit block (28) is arranged at the bottom of the filter plate (27), a guide rod (29) is arranged at one end of the limit block (28), the guide rod (29) is a telescopic structure, a second contact head (31) is arranged at one end of the guide rod (29), a buffer spring (30) is sleeved on the guide rod (29), one end of the buffer spring (30) is arranged on one side of the limit block (28), and the other end of the buffer spring (30) is arranged on one side of the second contact head (31), and a first contact head (26) is arranged at one end of the extension arm (25), the first contact head (26) is arranged in a trapezoidal shape, and the first contact head (26) is adapted to the second contact head (31).

10. A process for purifying a filtrate of a tetraacetyl phytosphingosine fermentation lysate, applied to the filtration of a filtrate of a tetraacetyl phytosphingosine fermentation lysate as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Preparation of fermentation lysate filtrate, fermentation of tetraacetyl phytosphingosine, discharge of fermentation broth from the fermentation tank, and preparation for the next step of mixing and filtration; S2: stirring and mixing, adding an auxiliary mixing component to the stirring tank (2), driving the stirring rod (4) through the driving motor (3), and using the threaded rod 9 and the moving collar 10 to improve the mixing efficiency; S3: Filtration, coarse filtration of the filtrate through the filter box (13) to remove larger particles, using a sliding filter plate (27) to ensure easy replacement of the filter screen; regulating the flow of the filtrate through the valve (14); S4: Solution treatment and collection, the refined tetraacetyl phytosphingosine solution, and the final purified solution can be further concentrated and dried as needed to finally obtain the purified tetraacetyl phytosphingosine product.

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